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Element Elevate Library Prep Workflow | Element Biosciences
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Elevate</div><div class="subnav__links" data-ref="subnav-links"><a href="/products/aviti" class="subnav__link" > AVITI </a><a href="/products/cloudbreak" class="subnav__link" > Cloudbreak </a><a href="/products/adept" class="subnav__link" > Adept </a><a href="/products/elevate" class="subnav__link -is-active" > Elevate </a><a href="/products/loopseq" class="subnav__link" > LoopSeq </a><a href="/products/elembio-cloud" class="subnav__link" > ElemBio Cloud </a></div></div></nav><main class="main" role="main"><div class="product -blue"><section class="product-hero"><div class="container"><div class="product-hero__row"><div class="product-hero__content"><ol class="breadcrumbs"><li> Products </li><li> Elevate </li></ol><script type="application/ld+json"> { "@context": "http://schema.org", "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Products" }, { "@type": "ListItem", "position": 2, "name": "Elevate" } ] } </script><h1 class="product-hero__heading">Element Elevate™ Library Prep Workflow</h1><div class="product-hero__intro"><p>The Element Elevate Library Prep Workflow offers native library prep solutions for a wide range of applications including whole-genome sequencing (WGS) and other DNA sequencing applications. Built on a foundation of efficient recovery and consistent results, the Elevate Workflow accommodates variable sample types and input amounts, including low input and low quality. Efficient, user-friendly protocols with onboard circularization minimize touchpoints and accelerate library prep time. </p></div><div class="product-hero__links"><a href="https://go.elementbiosciences.com/elevate-library-kit-lt-00005" class="button -white" rel="noreferrer noopener" target="_blank">See the DNA Specification Sheet</a></div></div><picture class="product-hero__photo"><img src="https://www.elementbiosciences.com/uploads/products/hero/_productHeroPhoto/elevate-hero-photo.png" width="1000" height="750" alt="Elevate hero photo" ></picture></div></div></section><section class="product-section -justified"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">Go End-to-End with Elevate</h2><div class="product-section__content"><div class="product-text -large"><p>The Elevate Workflow features a modular kit design for greater flexibility. Mix and match library prep, enrichment, and index kits to configure your ideal end-to-end prep. Workflow choices include: </p><ul><li>DNA library prep with or without PCR </li><li>Mechanical or enzymatic fragmentation of DNA samples</li><li>Multiplexing of up to 96 samples with 96 unique dual indexes (UDIs) for accurate read assignment</li><li>Standalone index and adapter plates for custom library prep </li><li>Size selection that calibrates insert sizes </li></ul></div></div></div></div></section><section class="product-section"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">Elevate Specifications</h2><div class="product-section__content"><div class="product-text"><p>Built to stringent specifications to support a variety of study requirements, DNA and RNA library prep kits prepare libraries for <a href="https://www.elementbiosciences.com/technology/avidite-base-chemistry">avidite base chemistry</a> sequencing in less than four hours. For complete compatibility information, see <a href="/product-compatibility">Product Compatibility</a>. </p></div><div class="product-table"><p class="product-table__tip">Swipe left in the table to show more columns.</p><table><tbody><tr><td><p><strong>Specification</strong></p></td><td><p><strong>Mechanical Kit</strong></p></td><td><p><strong>Enzymatic Kit</strong></p></td><td><p><strong>RNA Kit</strong></p></td></tr><tr><td><p>Kit size </p></td><td><p>24 reactions </p></td><td><p>24 reactions </p></td><td><p>24 reactions </p></td></tr><tr><td><p>Multiplexing </p></td><td><p>≤ 96 UDIs </p></td><td><p>≤ 96 UDIs </p></td><td><p>≤ 96 UDIs </p></td></tr><tr><td><p>Automation capability </p></td><td><p>Liquid handling robot </p></td><td><p>Liquid handling robot </p></td><td><p>Liquid handling robot </p></td></tr><tr><td><p>Input requirement </p></td><td><p>1–500 ng sheared genomic (gDNA) </p></td><td><p>1–500 ng gDNA </p></td><td><p>10–4000 ng RNA </p></td></tr><tr><td><p>Fragmentation method </p></td><td><p>Covaris shearing </p></td><td><p>Enzymatic </p></td><td><p>Enzymatic </p></td></tr><tr><td><p>Enrichment method </p></td><td><p>Not applicable </p></td><td><p>Not applicable </p></td><td><p>mRNA enrichment or rRNA depletion </p></td></tr><tr><td><p>PCR-free protocol time* </p></td><td><p>2 hours and 25 minutes </p></td><td><p>1 hour and 50 minutes </p></td><td><p>Not applicable </p></td></tr><tr><td><p>PCR-plus protocol time* </p></td><td><p>3 hours and 25 minutes </p></td><td><p>2 hours and 55 minutes </p></td><td><p>6 hours </p></td></tr><tr><td><p>Storage </p></td><td><p>-25°C to -15°C </p></td><td><p>-25°C to -15°C </p></td><td><p>-25°C to -15°C </p></td></tr></tbody></table><p class="product-footnote"><br /></p><p class="product-footnote">* Approximate total time that includes hands-on time</p></div></div></div></div></section><section class="product-section"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">Catalog Numbers</h2><div class="product-section__content"><div class="product-table"><p class="product-table__tip">Swipe left in the table to show more columns.</p><table><tbody><tr><td><p><strong>Product</strong></p></td><td><p><strong>Catalog #</strong></p></td></tr><tr><td colspan="2"><p><strong>DNA Library Prep</strong></p></td></tr><tr><td><p>Elevate Mechanical Library Prep Kit </p></td><td width="200"><p>830-00008 </p></td></tr><tr><td><p>Elevate Enzymatic Library Prep Kit </p></td><td><p>830-00009 </p></td></tr><tr><td><p>Elevate Long UDI Adapter Kit Set A </p></td><td><p>830-00010 </p></td></tr><tr><td><p>Elevate Index and Adapter Kit<br />* This item will no longer be sold through Element Biosciences. Please order equivalent items from <a href="https://www.idtdna.com/pages" target="_blank" rel="noopener">Integrated DNA Technologies</a> (IDT) Catalog # 10017037 (96rxn size) or Catalog # 10017036 (16rxn size)</p></td><td><p>830-00005 </p></td></tr></tbody></table></div></div></div></div></section><section class="product-section"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">Get In Touch</h2><div class="product-section__content"><form id="contact_us" method="post" class="form product-form" data-ref="form" data-handle="contact" data-recaptcha="v2_invisible" data-recaptcha-key="6Lf9AJ8fAAAAAJV8KV3IFsNp8pko0qr56kyvjO3c" data-recaptcha-lazy-load data-freeform data-id="8a06e8-form-q81yPZdRB-XalPvNPkE-j7LbzgJBX54HmWJLv61uHQn8Tvyb87FvF1aK9G46" data-disable-submit data-show-spinner data-auto-scroll data-show-loading-text data-loading-text="Sending..." data-success-message="Thank you for your contact request!" data-error-message="Sorry, there was an error submitting the form. 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