Identification and Quantification of Phenolic Compounds In Cassia tora Collected from Three Different Locations Using Ultra High Performance Liquid Chromatography – Electro Spray Ionization

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Quantification of Phenolic Compounds In Cassia tora Collected from Three Different Locations Using Ultra High Performance Liquid Chromatography – Electro Spray Ionization – Mass Spectrometry (UHPLC-ESI-MS-MS)</title> <meta name="description" content="Identification and Quantification of Phenolic Compounds In Cassia tora Collected from Three Different Locations Using Ultra High Performance Liquid Chromatography – Electro Spray Ionization – Mass Spectrometry (UHPLC-ESI-MS-MS)"> <meta name="keywords" content="Cassia tora, phenolic compounds, quantification, UHPLC-ESI-MS/MS"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" 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<div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 87358</div> </div> </div> </div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Identification and Quantification of Phenolic Compounds In Cassia tora Collected from Three Different Locations Using Ultra High Performance Liquid Chromatography – Electro Spray Ionization – Mass Spectrometry (UHPLC-ESI-MS-MS)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shipra%20Shukla">Shipra Shukla</a>, <a href="https://publications.waset.org/abstracts/search?q=Gaurav%20Chaudhary"> Gaurav Chaudhary</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20K.%20Tewari"> S. K. Tewari</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahesh%20Pal"> Mahesh Pal</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20K.%20Upreti"> D. K. Upreti</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cassia tora L. is widely distributed in tropical Asian countries, commonly known as sickle pod. Various parts of the plant are reported for their medicinal value due to presence of anthraquinones, phenolic compounds, emodin, β-sitosterol, and chrysophanol. Therefore a sensitive analytical procedure using UHPLC-ESI-MS/MS was developed and validated for simultaneous quantification of five phenolic compounds in leaf, stem and root extracts of Cassia tora. Rapid chromatographic separation of compounds was achieved on Acquity UHPLC BEH C18 column (50 mm×2.1 mm id, 1.7µm) column in 2.5 min. Quantification was carried out using negative electrospray ionization in multiple-reaction monitoring mode. The method was validated as per ICH guidelines and showed good linearity (r2 ≥ 0.9985) over the concentration range of 0.5-200 ng/mL. The intra- and inter-day precisions and accuracy were within RSDs ≤ 1.93% and ≤ 1.90%, respectively. The developed method was applied to investigate variation of five phenolic compounds in the three geographical collections. Results indicated significant variation among analyzed samples collected from different locations in India. <iframe src="https://publications.waset.org/abstracts/56791.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cassia%20tora" title="Cassia tora">Cassia tora</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20compounds" title=" phenolic compounds"> phenolic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=quantification" title=" quantification"> quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=UHPLC-ESI-MS%2FMS" title=" UHPLC-ESI-MS/MS"> UHPLC-ESI-MS/MS</a> </p> <a href="https://publications.waset.org/abstracts/56791/identification-and-quantification-of-phenolic-compounds-in-cassia-tora-collected-from-three-different-locations-using-ultra-high-performance-liquid-chromatography-electro-spray-ionization-mass-spectrometry-uhplc-esi-ms-ms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56791.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">269</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" 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Identification and Quantification of Phenolic Compounds In Cassia tora Collected from Three Different Locations Using Ultra High Performance Liquid Chromatography – Electro Spray Ionization – Mass Spectrometry (UHPLC-ESI-MS-MS)