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1%;padding:20px 0}.mm-vertical.header-plain #Top_bar .menu > li > ul.mfn-megamenu{width:100%!important;margin:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li{display:table-cell;float:none!important;width:10%;padding:0 15px;border-right:1px solid rgba(0,0,0,0.05)}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:980px!important;margin:0}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{margin:20px 0;padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-transparent #Top_bar .menu > li > a{margin:5px 0}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-top.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-bottom.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span:not(.description){border-right-width:0}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank{padding-left:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:none;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{display:none}#Header_creative #Top_bar .top_bar_right_wrapper{top:0}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0!important}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto;bottom:0}#Header_creative #Top_bar a#header_cart{display:inline-block;float:none;top:3px}#Header_creative #Top_bar a#search_button{display:inline-block;float:none;top:3px}#Header_creative #Top_bar .wpml-languages{display:inline-block;float:none;top:0}#Header_creative #Top_bar .wpml-languages.enabled:hover a.active{padding-bottom:9px}#Header_creative #Top_bar a.button.action_button{display:inline-block;float:none;top:16px;margin:0}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl.nice-scroll #Header_creative{margin-right:10px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul li.hover a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1130px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;filter:alpha(opacity = 97);-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{max-width:1130px;left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}.layout-boxed.header-boxed.nice-scroll #Top_bar.is-sticky{margin-left:-5px}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none}#Top_bar.is-sticky .top_bar_right{top:-4px;height:auto}#Top_bar.is-sticky .top_bar_right_wrapper{top:15px}.header-plain #Top_bar.is-sticky .top_bar_right_wrapper{top:0}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right a{color:rgba(255,255,255,.5)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.3);border-color:rgba(0,0,0,0.1)}}@media only screen and (min-width:768px) and (max-width:1130px){.header_placeholder{height:0!important}}@media only screen and (max-width:1129px){#Top_bar #menu{display:none;height:auto;width:100%;bottom:auto;top:100%;right:1px;position:absolute;margin:0}#Top_bar a.responsive-menu-toggle{display:block}#Top_bar #menu > ul{width:100%;float:left}#Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}#Top_bar #menu ul li a{padding:0 25px;margin:0;display:block;height:auto;line-height:normal;border:none}#Top_bar #menu ul li a:after{display:none}#Top_bar #menu ul li a 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ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/e220evolution-focused-ultrasonicator-500429"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, 1 to 8 sample batch processing</span></a></li><li id="menu-item-26913" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26913 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/e220-focused-ultrasonicator-500239"><span class="ubermenu-target-title ubermenu-target-text">E220</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, 1 to 96 sample batch preparation system</span></a></li></ul></li><li id="menu-item-26914" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26914 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/high-throughput-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">High-throughput Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26914 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26915" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26915 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220-plus-focused-ultrasonicator-500569"><span class="ubermenu-target-title ubermenu-target-text">LE220-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High-throughput, 1 to 96 tube capacity</span></a></li><li id="menu-item-26916" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26916 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220r-plus-focused-ultrasonicator-500578"><span class="ubermenu-target-title ubermenu-target-text">LE220R-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Robotic version of the LE220-plus</span></a></li><li id="menu-item-30065" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30065 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/le220rsc-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">LE220Rsc</span></a></li></ul></li><li id="menu-item-26917" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26917 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/on-deck-automation-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">On-Deck Automation Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26917 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26918" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26918 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/r230-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">R230</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">On-deck, next generation Focused-ultrasonicator</span></a></li></ul></li><li id="menu-item-26919" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26919 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/cryoprep"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP<sup>®</sup> Extraction Systems</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26919 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26920" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26920 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp01-cryoprep-manual-dry-pulverizer-500230"><span class="ubermenu-target-title ubermenu-target-text">CP01 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 15 mg to 2 g</span></a></li><li id="menu-item-26921" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26921 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp02-cryoprep-automated-dry-pulverizer-110v-500001"><span class="ubermenu-target-title ubermenu-target-text">CP02 Automated Dry Pulverizer</span></a></li><li id="menu-item-26922" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26922 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp03-cryoprep-manual-dry-pulverizer-500049"><span class="ubermenu-target-title ubermenu-target-text">CP03 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 2 to 5 g</span></a></li></ul></li><li id="menu-item-26923" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26923 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/sonolab-software"><span class="ubermenu-target-title ubermenu-target-text">SonoLab Software</span></a></li></ul></li><li id="menu-item-26927" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26927 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents"><span class="ubermenu-target-title ubermenu-target-text">Kits & Reagents</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26927 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-41083" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-41083 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truprep-protein/"><span class="ubermenu-target-title ubermenu-target-text">truPREP® Protein Kits</span></a></li><li id="menu-item-35108" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-35108 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truxtrac-ffpe-na-auto-96-kits"><span class="ubermenu-target-title ubermenu-target-text">truXTRAC® FFPE SMART Solutions</span></a></li><li id="menu-item-26931" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26931 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/cryoprep-0"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Starter Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cryoPREP Sample Tubes & Holders</span></a></li><li id="menu-item-26933" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26933 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/nucleic-acids-shearing-kits-and-buffers"><span class="ubermenu-target-title ubermenu-target-text">Nucleic Acids Shearing Kits and Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">truSHEAR, truPOP, DNA Verification Kits</span></a></li><li id="menu-item-26929" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26929 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-ffpe-kits"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> FFPE Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">FFPE Biomolecule Extraction Kits</span></a></li><li id="menu-item-26934" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26934 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-other"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cfDNA, DBS, MALDI-TOF</span></a></li><li id="menu-item-26935" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26935 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/protein-extraction-buffers"><span class="ubermenu-target-title ubermenu-target-text">Protein Extraction Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Native, Total Protein, & Detergent-free Buffers</span></a></li><li id="menu-item-26930" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26930 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truchip"><span class="ubermenu-target-title ubermenu-target-text">truChIP<sup>®</sup></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Chromatin Shearing Kits</span></a></li></ul></li><li id="menu-item-26936" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26936 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables"><span class="ubermenu-target-title ubermenu-target-text">Consumables – Tubes and Vials</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26936 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26938" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26938 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/afa-tube"><span class="ubermenu-target-title ubermenu-target-text">AFA-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Laser etched polymer vessel for high-throughput workflows</span></a></li><li id="menu-item-26939" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26939 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/microtube"><span class="ubermenu-target-title ubermenu-target-text">microTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Small sample volumes, 130 µl or less</span></a></li><li id="menu-item-26940" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26940 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/millitube"><span class="ubermenu-target-title ubermenu-target-text">milliTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">1 ml or 2 ml sample volume. Good for chromatin shearing</span></a></li><li id="menu-item-26942" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26942 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/other-afa-tubes"><span class="ubermenu-target-title ubermenu-target-text">Other AFA-TUBEs</span></a></li><li id="menu-item-26943" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26943 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/afa-process-vessels/"><span class="ubermenu-target-title ubermenu-target-text">AFA Process Vessels</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, low temperature processing</span></a></li><li id="menu-item-26944" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26944 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/g-tube"><span class="ubermenu-target-title ubermenu-target-text">g-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">DNA shearing fragments 6 to 20 kb</span></a></li></ul></li><li id="menu-item-26945" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26945 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Consumables</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26945 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26947" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26947 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables/tissuetube"><span class="ubermenu-target-title ubermenu-target-text">tissueTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Tissue processing devices</span></a></li></ul></li><li id="menu-item-26949" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26949 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories"><span class="ubermenu-target-title ubermenu-target-text">Accessories</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26949 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26952" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26952 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/automation-accessories"><span class="ubermenu-target-title ubermenu-target-text">Automation Accessories</span></a></li><li id="menu-item-41270" class="ubermenu-item ubermenu-item-type-post_type ubermenu-item-object-page ubermenu-item-41270 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/r230-accessories"><span class="ubermenu-target-title ubermenu-target-text">R230 Accessories</span></a></li><li id="menu-item-26953" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26953 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/m220-accessories"><span class="ubermenu-target-title ubermenu-target-text">M220 Accessories</span></a></li><li id="menu-item-26954" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26954 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/me220-accessories"><span class="ubermenu-target-title ubermenu-target-text">ME220 Accessories</span></a></li><li id="menu-item-30718" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30718 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/ml230-accessories"><span class="ubermenu-target-title ubermenu-target-text">ML230 Accessories</span></a></li><li id="menu-item-26955" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26955 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/s220-accessories"><span class="ubermenu-target-title ubermenu-target-text">S220 Accessories</span></a></li><li id="menu-item-26956" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26956 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-evolution-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i> Accessories</span></a></li><li id="menu-item-26957" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26957 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220 Accessories</span></a></li><li id="menu-item-26958" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26958 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/l-series-accessories"><span class="ubermenu-target-title ubermenu-target-text">L-Series Accessories</span></a></li><li id="menu-item-26959" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26959 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp01-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP01 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class="magento_menu_wrapper"> --> <!-- #secondary-menu --> <!-- </div> --> <div class="mob_logo"><div class="logo"><a id="logo" href="https://www.covaris.com" title="Covaris, LLC" data-height="60" data-padding="16"><img class="logo-main scale-with-grid" src="https://www.covaris.com/wp/wp-content/uploads/covaris-logo-colour.webp" data-retina="https://www.covaris.com/wp/wp-content/uploads/covaris-logo-colour.webp" data-height="152" alt="Covaris" /><img class="logo-sticky scale-with-grid" src="https://www.covaris.com/wp/wp-content/uploads/covaris-logo-colour.webp" data-retina="https://www.covaris.com/wp/wp-content/uploads/covaris-logo-colour.webp" data-height="152" alt="Covaris" /><img class="logo-mobile scale-with-grid" src="https://www.covaris.com/wp/wp-content/uploads/covaris-logo-colour.webp" data-retina="https://www.covaris.com/wp/wp-content/uploads/covaris-logo-colour.webp" data-height="152" alt="Covaris" /><img class="logo-mobile-sticky scale-with-grid" 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class="vc_custom_heading vc_do_custom_heading custom_h4 vc_custom_1582525217690" >Support</h4><div class="vc_wp_custommenu wpb_content_element"><div class="widget widget_nav_menu"><div class="menu-support-sidebar-container"><ul id="menu-support-sidebar" class="menu"><li id="menu-item-15014" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-15014"><a href="/contact-support/">Contact Support</a></li> <li id="menu-item-15015" class="menu-item menu-item-type-custom menu-item-object-custom current-menu-item menu-item-15015"><a href="/protocols/" aria-current="page">Protocols</a></li> <li id="menu-item-15028" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-15028"><a href="/technical-resources/">Technical Resources</a> <ul class="sub-menu"> <li id="menu-item-15029" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-15029"><a href="/application-technical-notes/">Application Notes</a></li> <li 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Prep for Mycobacterium" value="Sample Prep for Mycobacterium" name="covaris_filter"><label for="Sample Prep for Mycobacterium">Sample Prep for Mycobacterium</label> <br/> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> </div> </div> </div></div></div></div></div></div></div><div class="technical_res common_md-9 wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1587734923886"><div class="wpb_wrapper"><h1 style="color: #0a3f59;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h2 vc_custom_1584360515159" >Protocols</h1><h5 style="color: #4b4b4b;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1584360536094" >Covaris is continuously working to improve our protocols and they are therefore subject to change. Please check back often for the latest protocol. Covaris assumes no liability regarding the use of a protocol for applications in which it is not intended or with use of non-Covaris certified consumables.</h5><div class="vc_row wpb_row vc_inner vc_row-fluid resources_active vc_custom_1584711085749 vc_row-has-fill vc_row-o-equal-height vc_row-flex"><div class="protocol_heading wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner vc_custom_1584710703959"><div class="wpb_wrapper"><h6 style="color: #293e53;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1584427366313" >Protocols</h6></div></div></div><div class="protocol_filter wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner vc_custom_1585215078995"><div class="wpb_wrapper"><p>Sort By</p><select class="covaris_resources_filter"><option selected="selected" value="popularity">Popularity</option><option value="select">Name</option></select> <div class="wpb_text_column wpb_content_element vc_custom_1584710629225" > <div class="wpb_wrapper"> </div> </div> </div></div></div></div> <div class="wpb_raw_code wpb_raw_html wpb_content_element vc_custom_1585731841880" > <div class="wpb_wrapper"> <div class="protocol_filter_by"> <p>Filtered By:</p> <ul></ul> </div> </div> </div> <div class="wpb_text_column wpb_content_element vc_custom_1650940443348" > <div class="wpb_wrapper"> <div class="protocol_item"> <div class="protocol_filter_count"></div> <p>Items</p> </div> </div> </div> <div class="covaris_resource_content"> <li> <h4>Quick Guide: DNA Shearing with ME220 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010349.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19301" rel="nofollow" >Download PDF</a> </li> <li> <h4>Quick Guide: DNA Shearing with M220 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010252.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19300" rel="nofollow" >Download PDF</a> </li> <li> <h4>Quick Guide: DNA Shearing with S220 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010368.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19302" rel="nofollow" >Download PDF</a> </li> <li> <h4>Quick Guide: DNA Shearing with E220 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="/wp/wp-content/uploads/resources_pdf/pn_010308.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19303" rel="nofollow" >Download PDF</a> </li> <li> <h4>truChIP<sup>®</sup> Chromatin Shearing Kit</h4> <h6>Quick Guide</h6><h5> Application: Chromatin Shearing Protocols </h5> <a href="/wp/wp-content/uploads/resources_pdf/pn_010179.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19324" rel="nofollow" >Download PDF</a> </li> <li> <h4>Quick Guide: DNA Shearing with LE220-plus/LE220R-plus/LE220Rsc</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/2020/06/pn_010433.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19306" rel="nofollow" >Download PDF</a> </li> <li> <h4>Proteomics Quick Guide</h4> <h6>Quick Guide</h6><h5> Application: Proteomic Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010542.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="31377" rel="nofollow" >Download PDF</a> </li> <li> <h4>R230 DNA Shearing Protocols</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/pn_010528.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="28518" rel="nofollow">Download PDF</a> </li> <li> <h4>Quick Guide: DNA Shearing with ML230 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010522.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="28389" rel="nofollow" >Download PDF</a> </li> <li> <h4>Proteomics Protocols for Focused-Ultrasonicators</h4> <h6>Quick Guide</h6><h5> Application: Proteomic Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/Proteomics-Protocols-Yeast-Lysis-SuperB_5-2-14.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19347" rel="nofollow" >Download PDF</a> </li> <li> <h4>Tissue processing with the CP02 cryoPREP<sup>®</sup> Automated Dry Pulverizer</h4> <h6>Quick Guide</h6><h5> Application: Tissue Processing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010303.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19345" rel="nofollow" >Download PDF</a> </li> <li> <h4>truChIP<sup>®</sup> Chromatin Shearing Tissue Kit (PN 520237 and 520238)</h4> <h6>Quick Guide</h6><h5> Application: Chromatin Shearing Protocols </h5> <a href="/wp/wp-content/uploads/resources_pdf/pn_010385.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19319" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC FFPE total NA Ultra - Column Protocol</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="/wp/wp-content/uploads/resources_pdf/pn_010534.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="30255" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA Shearing with microTUBEs (<1.5kb fragments)</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/2020/04/pn_010296.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19318" rel="nofollow" >Download PDF</a> </li> <li> <h4>RNA Shearing with M220 and ME220 Focused-ultrasonicators</h4> <h6>Quick Guide</h6><h5> Application: RNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010183.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19344" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> FFPE DNA Plus Kit – Magnetic Beads</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="/wp/wp-content/uploads/pn_010466.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19337" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> FFPE total NA Kit - Column (25)</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010434.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19331" rel="nofollow" >Download PDF</a> </li> <li> <h4>Protocol truXTRAC® FFPE total NA (tNA) Ultra Kit – Magnetic Bead (25)</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010533.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="29788" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> FFPE RNA Plus Kit – Magnetic Beads</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="/wp/wp-content/uploads/2020/07/pn_010482.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19339" rel="nofollow" >Download PDF</a> </li> <li> <h4>RNA Shearing with S, E, and LE-Series Focused-ultrasonicators</h4> <h6>Quick Guide</h6><h5> Application: RNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010304.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19343" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> Protein Extraction Buffer SuperB</h4> <h6>Quick Guide</h6><h5> Application: Proteomic Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010390.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19349" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA Shearing with microTUBEs (<1.5kb fragments) S220/E220</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010305.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19309" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> FFPE total NA (tNA) Kit - Magnetic Bead Purification (25)</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="/wp/wp-content/uploads/resources_pdf/pn_010445.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19328" rel="nofollow" >Download PDF</a> </li> <li> <h4>Tissue processing with the CP01 cryoPREP<sup>®</sup> Manual Dry Pulverizer</h4> <h6>Quick Guide</h6><h5> Application: Tissue Processing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010458.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19346" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA Shearing Protocols for Ion Torrent Platforms with the M220 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010194.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19316" rel="nofollow" >Download PDF</a> </li> <li> <h4>truChIP<sup>®</sup> FFPE Chromatin Shearing Kit</h4> <h6>Quick Guide</h6><h5> Application: Chromatin Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010464.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19553" rel="nofollow" >Download PDF</a> </li> <li> <h4>Quick Guide: DNA Shearing with LE220 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010156.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19305" rel="nofollow" >Download PDF</a> </li> <li> <h4>Quick Guide: DNA Shearing with S2/E210 Focused-ultrasonicator</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010158.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19304" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA Shearing Quick Guide: microTUBE-15 with S220/E220 Focused-ultrasonicator.</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="/wp/wp-content/uploads/resources_pdf/pn_010253.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19317" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> FFPE total NA (tNA) Plus Kit - Magnetic Beads</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/pn_010451.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19329" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> Protein Extraction Buffer DF</h4> <h6>Quick Guide</h6><h5> Application: Proteomic Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010391.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19350" rel="nofollow" >Download PDF</a> </li> <li> <h4>truChIP<sup>®</sup> Ultra-Low Chromatin Shearing Kit</h4> <h6>Quick Guide</h6><h5> Application: Chromatin Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010255.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19322" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> FFPE total NA Plus Kit - Column</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010489.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19332" rel="nofollow" >Download PDF</a> </li> <li> <h4>truChIP<sup>®</sup> Native Chromatin Shearing Kit</h4> <h6>Quick Guide</h6><h5> Application: Chromatin Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010427.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19321" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> Protein Extraction Buffer N</h4> <h6>Quick Guide</h6><h5> Application: Proteomic Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010389.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19348" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC® FFPE total NA (tNA) Ultra Kit – Magnetic Bead (25) - KingFisher™ Duo Prime Purification System</h4> <h6>Quick Guide</h6><h5> </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010527.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="29870" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> Protein Extraction Buffer TP</h4> <h6>Quick Guide</h6><h5> Application: Proteomic Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010392.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19351" rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-Liposome™ Formation: HSPC/PEGDSPE2000/Cholesterol</h4> <h6>Quick Guide</h6><h5> Application: AFA-Liposome and AFA-nanoemulsions Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010234.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19353" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> FFPE total NA Kit - KingFisher™ Duo Prime Purification (25)</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010450.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19340" rel="nofollow" >Download PDF</a> </li> <li> <h4>KingFisher™ Duo Prime Purification in combination with the truXTRAC<sup>®</sup> cfDNA Kit</h4> <h6>Quick Guide</h6><h5> Application: cfDNA Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010443.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19366" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC™ FFPE DNA microTUBE Kit – Column Purification (25)</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/010178-Protocol-truXTRAC-FFPE-DNA-Kit.-Rev-O.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19334" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA miniTUBE – Clear</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010300.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19313" rel="nofollow" >Download PDF</a> </li> <li> <h4>Experienced User Quick Guide: truXTRAC<sup>®</sup> FFPE total Nucleic Acid Plus Kit –Magnetic Beads</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010510.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19330" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA miniTUBE – Red</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010301.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19314" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC™ FFPE microTUBE RNA Kit – Column Purification (25)</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010268.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19338" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA miniTUBE – Clear for 220 Series</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010306.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19310" rel="nofollow" >Download PDF</a> </li> <li> <h4>DNA miniTUBE- Blue</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010299.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19312" rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-Nanoemulsion™ : Rice Bran Oil in Water 2 mL</h4> <h6>Quick Guide</h6><h5> Application: AFA-Liposome and AFA-nanoemulsions Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010227.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19357" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC® FFPE total NA (tNA) Kit – Magnetic Bead Purification (25)</h4> <h6>Quick Guide</h6><h5> Application: AFA Technology, FFPE Extraction </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/pn_010445.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="39744" rel="nofollow" >Download PDF</a> </li> <li> <h4>truChIP® Chromatin Shearing Kit Protocol</h4> <h6>Quick Guide</h6><h5> Application: AFA Technology, Chromatin and ChIP </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010179.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="38575" rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-Liposome™ Formation: DOPE Based 2 mL</h4> <h6>Quick Guide</h6><h5> Application: AFA-Liposome and AFA-nanoemulsions Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010233.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19354" rel="nofollow" >Download PDF</a> </li> <li> <h4>Experienced User Quick Guide: truXTRAC<sup>®</sup> FFPE total Nucleic Acid Plus Kit – Column</h4> <h6>Quick Guide</h6><h5> Application: Nucleic Acid Extraction from FFPE Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/2020/07/pn_010511.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19333" rel="nofollow" >Download PDF</a> </li> <li> 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220 Series</h4> <h6>Quick Guide</h6><h5> Application: DNA Shearing Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010307.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19308" rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-Nanocrystal™ Production: Batch-scale (2mL) Indomethacin Preparation Protocol</h4> <h6>Quick Guide</h6><h5> Application: Crystallization Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010315.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19363" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC<sup>®</sup> MALDI-TOF Mycobacteria Kit</h4> <h6>Quick Guide</h6><h5> Application: Sample Prep for Mycobacterium </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010284.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19372" rel="nofollow" >Download PDF</a> </li> <li> <h4>truXTRAC™ FFPE DNA 8 microTUBE Strip 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rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-Liposome™ Formation: Hydrogenated Egg Lecithin 2 mL</h4> <h6>Quick Guide</h6><h5> Application: AFA-Liposome and AFA-nanoemulsions Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010235.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19355" rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-Nanoemulsion™: Rice Bran Oil in Water 300µ</h4> <h6>Quick Guide</h6><h5> Application: AFA-Liposome and AFA-nanoemulsions Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010230.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19358" rel="nofollow" >Download PDF</a> </li> <li> <h4>AFA-Liposome™ Formation: Phospholipon 90G 2mL</h4> <h6>Quick Guide</h6><h5> Application: AFA-Liposome and AFA-nanoemulsions Protocols </h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010236.pdf" target="_blank" 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args.slice() : args ]; if (this.isFiring) { this.fireStack.push(args); } else { this.fire(args); } return deferred; }, /** * Disable firing of new events */ disable: function () { this.isDisabled = true; }, /** * Enable firing of new events */ enable: function () { this.isDisabled = false; } }; } if(typeof(IASHistoryExtension) == "undefined"){ /** * IAS History Extension * An IAS extension to enable browser history * http://infiniteajaxscroll.com * * This file is part of the Infinite AJAX Scroll package * * Copyright 2014 Webcreate (Jeroen Fiege) */ var IASHistoryExtension = function (options) { options = jQuery.extend({}, this.defaults, options); this.ias = null; this.prevSelector = options.prev; this.prevUrl = null; this.listeners = { prev: new IASCallbacks() }; /** * @private * @param pageNum * @param scrollOffset * @param url */ this.onPageChange = function (pageNum, scrollOffset, url) { var state = {}; if (!window.history || !window.history.replaceState) { return; } history.replaceState(state, document.title, url); }; /** * @private * @param currentScrollOffset * @param scrollThreshold */ this.onScroll = function (currentScrollOffset, scrollThreshold) { var firstItemScrollThreshold = this.getScrollThresholdFirstItem(); if (!this.prevUrl) { return; } currentScrollOffset -= this.ias.$scrollContainer.height(); if (currentScrollOffset <= firstItemScrollThreshold) { this.prev(); } }; /** * Returns the url for the next page * * @private */ this.getPrevUrl = function (container) { if (!container) { container = this.ias.$container; } // always take the last matching item var prev_url = jQuery(this.prevSelector, container).last().attr('href'); if(typeof(prev_url) != 'undefined') { prev_url += '&ajaxscroll=1'; } else { prev_url = ''; } return prev_url; }; /** * Returns scroll threshold. This threshold marks the line from where * IAS should start loading the next page. * * @private * @return {number} */ this.getScrollThresholdFirstItem = function () { var $firstElement; $firstElement = this.ias.getFirstItem(); // if the don't have a first element, the DOM might not have been loaded, // or the selector is invalid if (0 === $firstElement.size()) { return -1; } return ($firstElement.offset().top); }; /** * Renders items * * @private * @param items * @param callback */ this.renderBefore = function (items, callback) { var ias = this.ias, $firstItem = ias.getFirstItem(), count = 0; ias.fire('render', [items]); jQuery(items).hide(); // at first, hide it so we can fade it in later $firstItem.before(items); jQuery(items).fadeIn(400, function () { if (++count < items.length) { return; } ias.fire('rendered', [items]); if (callback) { callback(); } }); }; return this; }; /** * @public */ IASHistoryExtension.prototype.initialize = function (ias) { var self = this; this.ias = ias; // expose the extensions listeners jQuery.extend(ias.listeners, this.listeners); // expose prev method ias.prev = function() { return self.prev(); }; this.prevUrl = this.getPrevUrl(); }; /** * Bind to events * * @public * @param ias */ IASHistoryExtension.prototype.bind = function (ias) { var self = this; ias.on('pageChange', jQuery.proxy(this.onPageChange, this)); ias.on('scroll', jQuery.proxy(this.onScroll, this)); ias.on('ready', function () { var currentScrollOffset = ias.getCurrentScrollOffset(ias.$scrollContainer), firstItemScrollThreshold = self.getScrollThresholdFirstItem(); currentScrollOffset -= ias.$scrollContainer.height(); if (currentScrollOffset <= firstItemScrollThreshold) { self.prev(); } }); }; /** * Load the prev page * * @public */ IASHistoryExtension.prototype.prev = function () { var url = this.prevUrl, self = this, ias = this.ias; if (!url) { return false; } ias.unbind(); var promise = ias.fire('prev', [url]); promise.done(function () { ias.load(url, function (data, items) { self.renderBefore(items, function () { self.prevUrl = self.getPrevUrl(data); ias.bind(); if (self.prevUrl) { self.prev(); } }); }); }); promise.fail(function () { ias.bind(); }); return true; }; /** * @public */ IASHistoryExtension.prototype.defaults = { prev: ".prev" }; } if(typeof(IASNoneLeftExtension) == "undefined"){ /** * IAS None Left Extension * An IAS extension to show a message when there are no more pages te load * http://infiniteajaxscroll.com * * This file is part of the Infinite AJAX Scroll package * * Copyright 2014 Webcreate (Jeroen Fiege) */ var IASNoneLeftExtension = function(options) { options = jQuery.extend({}, this.defaults, options); this.ias = null; this.uid = (new Date()).getTime(); this.html = (options.html).replace('{text}', options.text); /** * Shows none left message */ this.showNoneLeft = function() { var $element = jQuery(this.html).attr('id', 'ias_noneleft_' + this.uid), $lastItem = this.ias.getLastItem(); $lastItem.after($element); $element.fadeIn(); }; return this; }; /** * @public */ IASNoneLeftExtension.prototype.bind = function(ias) { this.ias = ias; ias.on('noneLeft', jQuery.proxy(this.showNoneLeft, this)); }; /** * @public */ IASNoneLeftExtension.prototype.defaults = { text: 'You reached the end.', html: '<div class="ias-noneleft" style="text-align: center;">{text}</div>' }; } if(typeof(IASPagingExtension) == "undefined"){ /** * IAS Paging Extension * An IAS extension providing additional events * http://infiniteajaxscroll.com * * This file is part of the Infinite AJAX Scroll package * * Copyright 2014 Webcreate (Jeroen Fiege) */ var IASPagingExtension = function() { this.ias = null; this.pagebreaks = [[0, document.location.toString()]]; this.lastPageNum = 1; this.enabled = true; this.listeners = { pageChange: new IASCallbacks() }; /** * Fires pageChange event * * @param currentScrollOffset * @param scrollThreshold */ this.onScroll = function(currentScrollOffset, scrollThreshold) { if (!this.enabled) { return; } var ias = this.ias, currentPageNum = this.getCurrentPageNum(currentScrollOffset), currentPagebreak = this.getCurrentPagebreak(currentScrollOffset), urlPage; if (this.lastPageNum !== currentPageNum) { urlPage = currentPagebreak[1]; ias.fire('pageChange', [currentPageNum, currentScrollOffset, urlPage]); } this.lastPageNum = currentPageNum; }; /** * Keeps track of pagebreaks * * @param url */ this.onNext = function(url) { var currentScrollOffset = this.ias.getCurrentScrollOffset(this.ias.$scrollContainer); this.pagebreaks.push([currentScrollOffset, url]); // trigger pageChange and update lastPageNum var currentPageNum = this.getCurrentPageNum(currentScrollOffset) + 1; this.ias.fire('pageChange', [currentPageNum, currentScrollOffset, url]); this.lastPageNum = currentPageNum; }; /** * Keeps track of pagebreaks * * @param url */ this.onPrev = function(url) { var self = this, ias = self.ias, currentScrollOffset = ias.getCurrentScrollOffset(ias.$scrollContainer), prevCurrentScrollOffset = currentScrollOffset - ias.$scrollContainer.height(), $firstItem = ias.getFirstItem(); this.enabled = false; this.pagebreaks.unshift([0, url]); ias.one('rendered', function() { // update pagebreaks for (var i = 1, l = self.pagebreaks.length; i < l; i++) { self.pagebreaks[i][0] = self.pagebreaks[i][0] + $firstItem.offset().top; } // trigger pageChange and update lastPageNum var currentPageNum = self.getCurrentPageNum(prevCurrentScrollOffset) + 1; ias.fire('pageChange', [currentPageNum, prevCurrentScrollOffset, url]); self.lastPageNum = currentPageNum; self.enabled = true; }); }; return this; }; /** * @public */ IASPagingExtension.prototype.initialize = function(ias) { this.ias = ias; // expose the extensions listeners jQuery.extend(ias.listeners, this.listeners); }; /** * @public */ IASPagingExtension.prototype.bind = function(ias) { try { ias.on('prev', jQuery.proxy(this.onPrev, this), this.priority); } catch (exception) {} ias.on('next', jQuery.proxy(this.onNext, this), this.priority); ias.on('scroll', jQuery.proxy(this.onScroll, this), this.priority); }; /** * Returns current page number based on scroll offset * * @param {number} scrollOffset * @returns {number} */ IASPagingExtension.prototype.getCurrentPageNum = function(scrollOffset) { for (var i = (this.pagebreaks.length - 1); i > 0; i--) { if (scrollOffset > this.pagebreaks[i][0]) { return i + 1; } } return 1; }; /** * Returns current pagebreak information based on scroll offset * * @param {number} scrollOffset * @returns {number}|null */ IASPagingExtension.prototype.getCurrentPagebreak = function(scrollOffset) { for (var i = (this.pagebreaks.length - 1); i >= 0; i--) { if (scrollOffset > this.pagebreaks[i][0]) { return this.pagebreaks[i]; } } return null; }; /** * @public * @type {number} */ IASPagingExtension.prototype.priority = 500; } if(typeof(IASSpinnerExtension) == "undefined"){ /** * IAS Spinner Extension * An IAS extension to show a spinner when loading * http://infiniteajaxscroll.com * * This file is part of the Infinite AJAX Scroll package * * Copyright 2014 Webcreate (Jeroen Fiege) */ var IASSpinnerExtension = function(options) { options = jQuery.extend({}, this.defaults, options); this.ias = null; this.uid = new Date().getTime(); this.src = options.src; this.html = (options.html).replace('{src}', this.src); /** * Shows spinner */ this.showSpinner = function() { var $spinner = this.getSpinner() || this.createSpinner(), $lastItem = this.ias.getLastItem(); $lastItem.after($spinner); $spinner.fadeIn(); }; /** * Shows spinner */ this.showSpinnerBefore = function() { var $spinner = this.getSpinner() || this.createSpinner(), $firstItem = this.ias.getFirstItem(); $firstItem.before($spinner); $spinner.fadeIn(); }; /** * Removes spinner */ this.removeSpinner = function() { if (this.hasSpinner()) { this.getSpinner().remove(); } }; /** * @returns {jQuery|boolean} */ this.getSpinner = function() { var $spinner = jQuery('#ias_spinner_' + this.uid); if ($spinner.size() > 0) { return $spinner; } return false; }; /** * @returns {boolean} */ this.hasSpinner = function() { var $spinner = jQuery('#ias_spinner_' + this.uid); return ($spinner.size() > 0); }; /** * @returns {jQuery} */ this.createSpinner = function() { var $spinner = jQuery(this.html).attr('id', 'ias_spinner_' + this.uid); $spinner.hide(); return $spinner; }; return this; }; /** * @public */ IASSpinnerExtension.prototype.bind = function(ias) { this.ias = ias; ias.on('next', jQuery.proxy(this.showSpinner, this)); try { ias.on('prev', jQuery.proxy(this.showSpinnerBefore, this)); } catch (exception) {} ias.on('render', jQuery.proxy(this.removeSpinner, this)); }; /** * @public */ IASSpinnerExtension.prototype.defaults = { src: 'data:image/gif;base64,R0lGODlhEAAQAPQAAP///wAAAPDw8IqKiuDg4EZGRnp6egAAAFhYWCQkJKysrL6+vhQUFJycnAQEBDY2NmhoaAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACH/C05FVFNDQVBFMi4wAwEAAAAh/hpDcmVhdGVkIHdpdGggYWpheGxvYWQuaW5mbwAh+QQJCgAAACwAAAAAEAAQAAAFdyAgAgIJIeWoAkRCCMdBkKtIHIngyMKsErPBYbADpkSCwhDmQCBethRB6Vj4kFCkQPG4IlWDgrNRIwnO4UKBXDufzQvDMaoSDBgFb886MiQadgNABAokfCwzBA8LCg0Egl8jAggGAA1kBIA1BAYzlyILczULC2UhACH5BAkKAAAALAAAAAAQABAAAAV2ICACAmlAZTmOREEIyUEQjLKKxPHADhEvqxlgcGgkGI1DYSVAIAWMx+lwSKkICJ0QsHi9RgKBwnVTiRQQgwF4I4UFDQQEwi6/3YSGWRRmjhEETAJfIgMFCnAKM0KDV4EEEAQLiF18TAYNXDaSe3x6mjidN1s3IQAh+QQJCgAAACwAAAAAEAAQAAAFeCAgAgLZDGU5jgRECEUiCI+yioSDwDJyLKsXoHFQxBSHAoAAFBhqtMJg8DgQBgfrEsJAEAg4YhZIEiwgKtHiMBgtpg3wbUZXGO7kOb1MUKRFMysCChAoggJCIg0GC2aNe4gqQldfL4l/Ag1AXySJgn5LcoE3QXI3IQAh+QQJCgAAACwAAAAAEAAQAAAFdiAgAgLZNGU5joQhCEjxIssqEo8bC9BRjy9Ag7GILQ4QEoE0gBAEBcOpcBA0DoxSK/e8LRIHn+i1cK0IyKdg0VAoljYIg+GgnRrwVS/8IAkICyosBIQpBAMoKy9dImxPhS+GKkFrkX+TigtLlIyKXUF+NjagNiEAIfkECQoAAAAsAAAAABAAEAAABWwgIAICaRhlOY4EIgjH8R7LKhKHGwsMvb4AAy3WODBIBBKCsYA9TjuhDNDKEVSERezQEL0WrhXucRUQGuik7bFlngzqVW9LMl9XWvLdjFaJtDFqZ1cEZUB0dUgvL3dgP4WJZn4jkomWNpSTIyEAIfkECQoAAAAsAAAAABAAEAAABX4gIAICuSxlOY6CIgiD8RrEKgqGOwxwUrMlAoSwIzAGpJpgoSDAGifDY5kopBYDlEpAQBwevxfBtRIUGi8xwWkDNBCIwmC9Vq0aiQQDQuK+VgQPDXV9hCJjBwcFYU5pLwwHXQcMKSmNLQcIAExlbH8JBwttaX0ABAcNbWVbKyEAIfkECQoAAAAsAAAAABAAEAAABXkgIAICSRBlOY7CIghN8zbEKsKoIjdFzZaEgUBHKChMJtRwcWpAWoWnifm6ESAMhO8lQK0EEAV3rFopIBCEcGwDKAqPh4HUrY4ICHH1dSoTFgcHUiZjBhAJB2AHDykpKAwHAwdzf19KkASIPl9cDgcnDkdtNwiMJCshACH5BAkKAAAALAAAAAAQABAAAAV3ICACAkkQZTmOAiosiyAoxCq+KPxCNVsSMRgBsiClWrLTSWFoIQZHl6pleBh6suxKMIhlvzbAwkBWfFWrBQTxNLq2RG2yhSUkDs2b63AYDAoJXAcFRwADeAkJDX0AQCsEfAQMDAIPBz0rCgcxky0JRWE1AmwpKyEAIfkECQoAAAAsAAAAABAAEAAABXkgIAICKZzkqJ4nQZxLqZKv4NqNLKK2/Q4Ek4lFXChsg5ypJjs1II3gEDUSRInEGYAw6B6zM4JhrDAtEosVkLUtHA7RHaHAGJQEjsODcEg0FBAFVgkQJQ1pAwcDDw8KcFtSInwJAowCCA6RIwqZAgkPNgVpWndjdyohACH5BAkKAAAALAAAAAAQABAAAAV5ICACAimc5KieLEuUKvm2xAKLqDCfC2GaO9eL0LABWTiBYmA06W6kHgvCqEJiAIJiu3gcvgUsscHUERm+kaCxyxa+zRPk0SgJEgfIvbAdIAQLCAYlCj4DBw0IBQsMCjIqBAcPAooCBg9pKgsJLwUFOhCZKyQDA3YqIQAh+QQJCgAAACwAAAAAEAAQAAAFdSAgAgIpnOSonmxbqiThCrJKEHFbo8JxDDOZYFFb+A41E4H4OhkOipXwBElYITDAckFEOBgMQ3arkMkUBdxIUGZpEb7kaQBRlASPg0FQQHAbEEMGDSVEAA1QBhAED1E0NgwFAooCDWljaQIQCE5qMHcNhCkjIQAh+QQJCgAAACwAAAAAEAAQAAAFeSAgAgIpnOSoLgxxvqgKLEcCC65KEAByKK8cSpA4DAiHQ/DkKhGKh4ZCtCyZGo6F6iYYPAqFgYy02xkSaLEMV34tELyRYNEsCQyHlvWkGCzsPgMCEAY7Cg04Uk48LAsDhRA8MVQPEF0GAgqYYwSRlycNcWskCkApIyEAOwAAAAAAAAAAAA==', html: '<div class="ias-spinner" style="text-align: center;"><img src="{src}"/></div>' }; } if(typeof(IASTriggerExtension) == "undefined"){ /** * IAS Trigger Extension * An IAS extension to show a trigger link to load the next page * http://infiniteajaxscroll.com * * This file is part of the Infinite AJAX Scroll package * * Copyright 2014 Webcreate (Jeroen Fiege) */ var IASTriggerExtension = function(options) { options = jQuery.extend({}, this.defaults, options); this.ias = null; this.html = (options.html).replace('{text}', options.text); this.htmlPrev = (options.htmlPrev).replace('{text}', options.textPrev); this.enabled = true; this.count = 0; this.offset = options.offset; this.$triggerNext = null; this.$triggerPrev = null; /** * Shows trigger for next page */ this.showTriggerNext = function() { if (!this.enabled) { return true; } if (false === this.offset || ++this.count < this.offset) { return true; } var $trigger = this.$triggerNext || (this.$triggerNext = this.createTrigger(this.next, this.html)); var $lastItem = this.ias.getLastItem(); $lastItem.after($trigger); $trigger.fadeIn(); return false; }; /** * Shows trigger for previous page */ this.showTriggerPrev = function() { if (!this.enabled) { return true; } var $trigger = this.$triggerPrev || (this.$triggerPrev = this.createTrigger(this.prev, this.htmlPrev)); var $firstItem = this.ias.getFirstItem(); $firstItem.before($trigger); $trigger.fadeIn(); return false; }; /** * @param clickCallback * @returns {*|jQuery} * @param {string} html */ this.createTrigger = function(clickCallback, html) { var uid = (new Date()).getTime(), $trigger; html = html || this.html; $trigger = jQuery(html).attr('id', 'ias_trigger_' + uid); $trigger.hide(); $trigger.on('click', jQuery.proxy(clickCallback, this)); return $trigger; }; return this; }; /** * @public * @param {object} ias */ IASTriggerExtension.prototype.bind = function(ias) { var self = this; this.ias = ias; try { ias.on('prev', jQuery.proxy(this.showTriggerPrev, this), this.priority); } catch (exception) {} ias.on('next', jQuery.proxy(this.showTriggerNext, this), this.priority); ias.on('rendered', function () { self.enabled = true; }, this.priority); }; /** * @public */ IASTriggerExtension.prototype.next = function() { this.enabled = false; this.ias.unbind(); if (this.$triggerNext) { this.$triggerNext.remove(); this.$triggerNext = null; } this.ias.next(); }; /** * @public */ IASTriggerExtension.prototype.prev = function() { this.enabled = false; this.ias.unbind(); if (this.$triggerPrev) { this.$triggerPrev.remove(); this.$triggerPrev = null; } this.ias.prev(); }; /** * @public */ IASTriggerExtension.prototype.defaults = { text: 'Load more items', html: '<div class="ias-trigger ias-trigger-next" style="text-align: center; cursor: pointer;"><a>{text}</a></div>', textPrev: 'Load previous items', htmlPrev: '<div class="ias-trigger ias-trigger-prev" style="text-align: center; cursor: 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