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gustatory receptor</title> <meta name="description" content="Search results for: insect gustatory receptor"> <meta name="keywords" content="insect gustatory receptor"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="insect gustatory receptor"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 783</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: insect gustatory receptor</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">783</span> Detection of Nutrients Using Honeybee-Mimic Bioelectronic Tongue Systems</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Soo%20Ho%20Lim">Soo Ho Lim</a>, <a href="https://publications.waset.org/abstracts/search?q=Minju%20Lee"> Minju Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Dong%20In%20Kim"> Dong In Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Gi%20Youn%20Han"> Gi Youn Han</a>, <a href="https://publications.waset.org/abstracts/search?q=Seunghun%20Hong"> Seunghun Hong</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyung%20Wook%20Kwon"> Hyung Wook Kwon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We report a floating electrode-based bioelectronic tongue mimicking honeybee taste systems for the detection and discrimination of various nutrients. Here, carbon nanotube field effect transistors with floating electrodes (CNT-FET) were hybridized with nanovesicles containing honeybee nutrient receptors, gustatory receptors of Apis mellifera. This strategy enables us to detect nutrient substance with a high sensitivity and selectivity. It could also be utilized for the detection of nutrients in liquid food. This floating electrode-based bioelectronic tongue mimicking insect taste systems can be a simple, but highly effective strategy in many different basic research areas about sensory systems. Moreover, our research provides opportunities to develop various applications such as food screening, and it also can provide valuable insights on insect taste systems. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=taste%20system" title="taste system">taste system</a>, <a href="https://publications.waset.org/abstracts/search?q=CNT-FET" title=" CNT-FET"> CNT-FET</a>, <a href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor" title=" insect gustatory receptor"> insect gustatory receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=biolelectronic%20tongue" title=" biolelectronic tongue"> biolelectronic tongue</a> </p> <a href="https://publications.waset.org/abstracts/84686/detection-of-nutrients-using-honeybee-mimic-bioelectronic-tongue-systems" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84686.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">218</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">782</span> Giving Gustatory Aesthetics Its Place at the Table</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Brock%20Decker">Brock Decker</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Vision and hearing have been given metaphysical, epistemic, moral and aesthetic preference over the gustatory senses since the very beginnings of Western philosophy. This unjustified prejudice has directed philosophical inquiry away from taste and smell and the values and interests of those concerned with them. The metaphysical and epistemic prejudices that have hindered work in this field are confronted by accepting an oblique invitation from David Hume to pursue a gustatory aesthetics of taste. A framework for further discussion of gustatory experience is added by arguing that taste and smell are cognitively configurable senses capable of bifurcated intentionality and that the taste perception of states of affairs is influenced both by culture and personal preference. Taste perceptions are revealed to admit an aesthetic standard. Using both a Humean aesthetic and a Brillat-Savarin-inspired understanding of taste can explain and discriminate between untrained and expert aesthetic taste experiences and contribute a perspective free from traditional prejudice for future work in the aesthetics of taste. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aesthetics" title="aesthetics">aesthetics</a>, <a href="https://publications.waset.org/abstracts/search?q=Hume" title=" Hume"> Hume</a>, <a href="https://publications.waset.org/abstracts/search?q=Korsmeyer" title=" Korsmeyer"> Korsmeyer</a>, <a href="https://publications.waset.org/abstracts/search?q=taste" title=" taste"> taste</a>, <a href="https://publications.waset.org/abstracts/search?q=Scruton" title=" Scruton"> Scruton</a> </p> <a href="https://publications.waset.org/abstracts/162662/giving-gustatory-aesthetics-its-place-at-the-table" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162662.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">62</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">781</span> Plant Mediated RNAi Approach to Knock Down Ecdysone Receptor Gene of Colorado Potato Beetle</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tahira%20Hussain">Tahira Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Ilhom%20Rahamkulov"> Ilhom Rahamkulov</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Aasim"> Muhammad Aasim</a>, <a href="https://publications.waset.org/abstracts/search?q=Ugur%20Pirlak"> Ugur Pirlak</a>, <a href="https://publications.waset.org/abstracts/search?q=Emre%20Aksoy"> Emre Aksoy</a>, <a href="https://publications.waset.org/abstracts/search?q=Mehmet%20Emin%20Caliskan"> Mehmet Emin Caliskan</a>, <a href="https://publications.waset.org/abstracts/search?q=Allah%20Bakhsh"> Allah Bakhsh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> RNA interference (RNAi) has proved its usefulness in functional genomic research on insects recently and is considered potential strategy in crop improvement for the control of insect pests. The different insect pests incur significant losses to potato yield worldwide, Colorado Potato Beetle (CPB) being most notorious one. The present study focuses to knock down highly specific 20-hydroxyecdysone hormone-receptor complex interaction by using RNAi approach to silence Ecdysone receptor (EcR) gene of CPB in transgenic potato plants expressing dsRNA of EcR gene. The partial cDNA of Ecdysone receptor gene of CPB was amplified using specific primers in sense and anti-sense orientation and cloned in pRNAi-GG vector flanked by an intronic sequence (pdk). Leaf and internodal explants of Lady Olympia, Agria and Granola cultivars of potato were infected with Agrobacterium strain LBA4404 harboring plasmid pRNAi-CPB, pRNAi-GFP (used as control). Neomycin phosphotransferase (nptII) gene was used as a plant selectable marker at a concentration of 100 mg L⁻¹. The primary transformants obtained have shown proper integration of T-DNA in plant genome by standard molecular analysis like polymerase chain reaction (PCR), real-time PCR, Sothern blot. The transgenic plants developed out of these cultivars are being evaluated for their efficacy against larvae as well adults of CPB. The transgenic lines are expected to inhibit expression of EcR protein gene, hindering their molting process, hence leading to increased potato yield. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plant%20mediated%20RNAi" title="plant mediated RNAi">plant mediated RNAi</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20strategy" title=" molecular strategy"> molecular strategy</a>, <a href="https://publications.waset.org/abstracts/search?q=ecdysone%20receptor" title=" ecdysone receptor"> ecdysone receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=insect%20metamorphosis" title=" insect metamorphosis"> insect metamorphosis</a> </p> <a href="https://publications.waset.org/abstracts/93300/plant-mediated-rnai-approach-to-knock-down-ecdysone-receptor-gene-of-colorado-potato-beetle" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/93300.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">170</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">780</span> Morphological Comparison of the Gustatory Papillae of New Zealand White Rabbits (Oryctolagus cuniculus) and Egyptian Fruit Bats (Rousettus aegyptiacus) Using Scanning Electron Microscopic Examinations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Abumandour">Mohamed Abumandour</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This research presents a comparison of the morphological structure of the gustatory papillae in New Zealand white rabbits as domestic mammals and Egyptian fruit bats as wild mammals. In this study, the tongues of adult healthy New Zealand white rabbits and Egyptian fruit bats of both sexes were used. In the New Zealand white rabbits, there are three types of the gustatory papillae; fungiform, foliate and circumvallate papillae while the Egyptian fruit bats tongue contain only two types; fungiform and circumvallate papillae. In New Zealand white rabbits, there only one subtype is the round shape fungiform papillae while in Egyptian fruit bats, there are two subtypes; small rectangular fungiform papillae and large round fungiform papillae. In New Zealand white rabbits, there only two circumvallate papillae while in Egyptian fruit bats, there are three papillae. The shape, size, number, and distribution of the lingual papillae were varied according to their location within the tongue (region-specific) in relation to the feeding habits, strategies for obtaining food, climate conditions, and types of food particles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=morphology" title="morphology">morphology</a>, <a href="https://publications.waset.org/abstracts/search?q=circumvallate%20papillae" title=" circumvallate papillae"> circumvallate papillae</a>, <a href="https://publications.waset.org/abstracts/search?q=fungiform%20papillae" title=" fungiform papillae"> fungiform papillae</a>, <a href="https://publications.waset.org/abstracts/search?q=foliate%20papillae" title=" foliate papillae"> foliate papillae</a> </p> <a href="https://publications.waset.org/abstracts/61003/morphological-comparison-of-the-gustatory-papillae-of-new-zealand-white-rabbits-oryctolagus-cuniculus-and-egyptian-fruit-bats-rousettus-aegyptiacus-using-scanning-electron-microscopic-examinations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61003.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">240</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">779</span> Date Palm Insects and Mite Pests at Biskra Oasis, South Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20Tarai">N. Tarai</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Seighi"> S. Seighi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Doumandji"> S. Doumandji</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The date palm trees Phoenix dactylifera L. are subject to infestation with a variety of insect pests and mite associated, the Carob moth Ectomyelois ceatoniae (Zeller)(Lepidoptera, Pyralidae) is a key pest. Survey of the insect and mite pests associated with date palm trees in the seven stations at Biskra Oasis, throughout two successive years, from October 2011 until September 2012 revealed twelve insect pests belonging to ten families and six orders in addition to one mite belonging to one family from order Acari. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=date%20palm" title="date palm">date palm</a>, <a href="https://publications.waset.org/abstracts/search?q=insect" title=" insect"> insect</a>, <a href="https://publications.waset.org/abstracts/search?q=pests" title=" pests"> pests</a>, <a href="https://publications.waset.org/abstracts/search?q=infestation" title=" infestation"> infestation</a>, <a href="https://publications.waset.org/abstracts/search?q=mit" title=" mit"> mit</a>, <a href="https://publications.waset.org/abstracts/search?q=Biskra" title=" Biskra"> Biskra</a>, <a href="https://publications.waset.org/abstracts/search?q=Oasis" title=" Oasis "> Oasis </a> </p> <a href="https://publications.waset.org/abstracts/3003/date-palm-insects-and-mite-pests-at-biskra-oasis-south-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3003.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">455</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">778</span> Signaling of Leucine-Rich-Repeat Receptor-Like Kinases in Higher Plants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Man-Ho%20Oh">Man-Ho Oh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Membrane localized Leucine-Rich-Repeat Receptor-Like Kinases (LRR-RLKs) play crucial roles in plant growth and abiotic/biotic stress responses in higher plants including Arabidopsis and Brassica species. Among several Receptor-Like Kinases (RLKs), Leucine-Rich-Repeat Receptor-Like-Kinases (LRR-RLKs) are the major group of genes that play crucial roles related to growth, development and stress conditions in plant system. Since it is involved in several functional roles, it seems to be very important to investigate their roles in higher plants. We are particularly interested in brassinosteroid (BR) signaling, which is mediated by the BRASSINOSTEROID INSENSITIVE 1 (BRI1) receptor kinase and its co-receptor, BRI1-ASSOCIATED KINASE 1 (BAK1). Autophosphorylation of receptor kinases is recognized to be an important process in activation of signaling in higher plants. Although the plant receptors are generally classified as Ser/Thr protein kinases, many other receptor kinases including BRI1 and BAK1 are shown to autophosphorylate on Tyr residues in addition to Ser/Thr. As an interesting result, we determined that several 14-3-3 regulatory proteins bind to BRI1-CD and are phosphorylated by several receptor kinases in vitro, suggesting that BRI1 is critical for diverse signaling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=autophosphorylation" title="autophosphorylation">autophosphorylation</a>, <a href="https://publications.waset.org/abstracts/search?q=brassinosteroid" title=" brassinosteroid"> brassinosteroid</a>, <a href="https://publications.waset.org/abstracts/search?q=BRASSINOSTEROID%20INSENSITIVE%201" title=" BRASSINOSTEROID INSENSITIVE 1"> BRASSINOSTEROID INSENSITIVE 1</a>, <a href="https://publications.waset.org/abstracts/search?q=BRI1-ASSOCIATED%20KINASE%201" title=" BRI1-ASSOCIATED KINASE 1"> BRI1-ASSOCIATED KINASE 1</a>, <a href="https://publications.waset.org/abstracts/search?q=Leucine-Rich-Repeat%20Receptor-Like%20Kinases%20%28LRR-RLKs%29" title=" Leucine-Rich-Repeat Receptor-Like Kinases (LRR-RLKs)"> Leucine-Rich-Repeat Receptor-Like Kinases (LRR-RLKs)</a> </p> <a href="https://publications.waset.org/abstracts/76545/signaling-of-leucine-rich-repeat-receptor-like-kinases-in-higher-plants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76545.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">224</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">777</span> Insect Diversity Assessment of Maize Crop (Zea mays L.) by Using Sweep Net, Pitfall Trap and Plant Inspection Methods </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Naeem%20Mushtaq">Muhammad Naeem Mushtaq</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Arshad"> Muhammad Arshad</a>, <a href="https://publications.waset.org/abstracts/search?q=Shahid%20Majeed"> Shahid Majeed</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Maize is known as queen of cereals because of its highest genetic yield potential and multipurpose characteristics in human being and animal diet. Maize crop visited by many major, minor, visitors and sporadic insect pests. This study was conducted during 2014 to evaluate the richness and evenness of these insect pests and their interaction with metrological conditions at University of Agriculture, Faisalabad. In this experiment, two localities were selected; one was treated with pesticide and second was untreated. Maize field visited by many insect pests. Those insect pests were collected by using three collection method: sweep net, pitfall trap and plant inspection. The data was collected weekly interval from August to October and statistically analyzed by using Shannon Index which showed the results of insect pest richness and evenness. The value of Shannon Index was higher with the increase in number of species and abundance of insects. Camponotus nearcticus was most abundant in sweep net and pitfall trap method while Rhopalosiphum maidis was abundant in plant inspection method. Temperature was negatively co-relate with the insect population in all three collection methods while the relative humidity and rainfall had varying results. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=abundance" title="abundance">abundance</a>, <a href="https://publications.waset.org/abstracts/search?q=evenness" title=" evenness"> evenness</a>, <a href="https://publications.waset.org/abstracts/search?q=maize" title=" maize"> maize</a>, <a href="https://publications.waset.org/abstracts/search?q=richness" title=" richness"> richness</a> </p> <a href="https://publications.waset.org/abstracts/99026/insect-diversity-assessment-of-maize-crop-zea-mays-l-by-using-sweep-net-pitfall-trap-and-plant-inspection-methods" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99026.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">220</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">776</span> Insect Infestation of Two Varieties of Cowpea Seeds (Vigna Unguiculata L.Walp) Stored at Sokoto Central Market Grainaries</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Jatau">A. Jatau</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20M.%20Bandiya"> H. M. Bandiya</a>, <a href="https://publications.waset.org/abstracts/search?q=Q.%20Majeed"> Q. Majeed</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20A.%20Yahaya"> M. A. Yahaya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An investigation on the insect infestation of stored seeds of cowpea seeds varieties (Sokoto Loacal and Kanannado) was carried out in Sokoto central market, Sokoto. Two insects' species, Callosobrunchus maculatus and Callosobrunchus chinensis were found on the stored seeds with C. maculutus found to be the most prevalent. The rate of infestation of the cowpea seeds by the two insect species were significantly (P< 0.05) higher in Sokoto local than in Kanannado variety. The result shows that kanannado variety is more resistance to cowpea seeds weevils, hence should be used for long storage in Sokoto. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=insect" title="insect">insect</a>, <a href="https://publications.waset.org/abstracts/search?q=infestation" title=" infestation"> infestation</a>, <a href="https://publications.waset.org/abstracts/search?q=cowpea%20seeds" title=" cowpea seeds"> cowpea seeds</a>, <a href="https://publications.waset.org/abstracts/search?q=grainaries" title=" grainaries"> grainaries</a> </p> <a href="https://publications.waset.org/abstracts/11661/insect-infestation-of-two-varieties-of-cowpea-seeds-vigna-unguiculata-lwalp-stored-at-sokoto-central-market-grainaries" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/11661.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">387</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">775</span> Entomopathogenic Bacteria as Biological Control Agents: Review Paper</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tadesse%20Kebede%20Dabsu">Tadesse Kebede Dabsu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Insect pest is one the major limiting factor for sustainable food production. To overtake insect pest problem, since Second World War, producers have used excessive insecticide for insect pest management. However, in the era of 21st Century, the excessive use of insecticide caused insect resistant, insecticide bioaccumulation, insecticide hazard to environment, human health problem, and the like. Due to these problems, research efforts have been focused on the development of environmental free sustainable insect pest management method. To minimize all above mentioned risk utilizing of biological control such as entomopathogenicmicroorganism include bacteria, virus, fungus, and their productsare the best option for suppress insect population below certain density level. The objective of this review was to review the updated available studies and recent developments on the entomopathogenic bacteria (EPB) as biological control of insect pest and challenge of using them for control of insect pest. EPB’s mechanisms of insecticidal activities, type, taxonomy, and history are included in this paper body. EPB has been successfully used for the suppression of populations of insect pests. Controlling of harmful insect by entomopathogenic bacteria is an effective, low bioaccumulation in environment and food, very specific, reduce resistance risk in insect pest, economically and sustainable method of major insect pest management method. Identified and reported as potential major common type of entomopathogenic bacteria include Bacillus thuringiensis, Photorhabdus sp., Xenorhabdus spp.Walbachiaspp, Actinomycetesspp.etc. These bacteria being enter into insect body through natural opening or by vector release toxin protein inside of insect and disrupt the cell’s content cause natural mortality under natural condition. As per reported by different scientists, insect orders like Lepidoptera, Hemiptera, Hymenoptera, Coleoptera, and Dipterahave been successful controlled by entomopathogenic bacteria. As per coming across in different scientific research journals, much of the work was emphasised on Bacillus thuringiensisbsp. Therefore, for commercial production like Bacillus thuringiensi, detail research should be done on other bacteria species. The efficacy and practical application of EPB are restricted to some crops and greenhouse area, but their field application at farmers’ level very less. So still much work needs to be done to the practical application of the EPB at widely application. Their efficacy, pathogenicity, and host range test should be tested under environmental condition. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=insect%20pest" title="insect pest">insect pest</a>, <a href="https://publications.waset.org/abstracts/search?q=entomopathogenic%20bacteria" title=" entomopathogenic bacteria"> entomopathogenic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=biological%20control" title=" biological control"> biological control</a>, <a href="https://publications.waset.org/abstracts/search?q=agent" title=" agent"> agent</a> </p> <a href="https://publications.waset.org/abstracts/145140/entomopathogenic-bacteria-as-biological-control-agents-review-paper" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/145140.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">774</span> Conformational Switch of hRAGE upon Self-Association</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ikhlas%20Ahmed">Ikhlas Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamillah%20Zamoon"> Jamillah Zamoon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The human receptor for advanced glycation end product is a plasma membrane receptor with an intrinsically disordered region. The protein consists of three extracellular domains, a single membrane spanning transmembrane domain, and a cytosolic domain which is intrinsically disordered and responsible for signaling. The disordered nature of the cytosolic domain allows it to be dynamic in solution. This receptor self-associates to higher forms. The association is triggered by ligand, metal or by the extracellular domain. Fluorescence spectroscopy technique is used to test the self-association of the different concentrations of the cytosolic domain. This work has concluded that the cytosolic domain of this receptor also self-associates. Moreover, the self-association does not require ligand or metal. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fluorescence%20spectroscopy" title="fluorescence spectroscopy">fluorescence spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=hRAGE" title=" hRAGE"> hRAGE</a>, <a href="https://publications.waset.org/abstracts/search?q=IDP" title=" IDP"> IDP</a>, <a href="https://publications.waset.org/abstracts/search?q=Self-association" title=" Self-association"> Self-association</a> </p> <a href="https://publications.waset.org/abstracts/44509/conformational-switch-of-hrage-upon-self-association" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44509.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">361</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">773</span> Using of Bimolecular Fluorescence Complementation (BiFC) Assays to Study Homo and/ or Heterodimerization of Laminin Receptor 37 LRP/ 67 LR with Galectin-3</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fulwah%20Alqahtani">Fulwah Alqahtani</a>, <a href="https://publications.waset.org/abstracts/search?q=Jafar%20Mahdavi"> Jafar Mahdavi</a>, <a href="https://publications.waset.org/abstracts/search?q=Lee%20Weldon"> Lee Weldon</a>, <a href="https://publications.waset.org/abstracts/search?q=Nick%20Holliday"> Nick Holliday</a>, <a href="https://publications.waset.org/abstracts/search?q=Dlawer%20Ala%27Aldeen"> Dlawer Ala'Aldeen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There are two isoforms of laminin receptor; monomeric 37 kDa laminin receptor precursor (37 LRP) and mature 67 kDa laminin receptor (67 LR). The relationship between the 67 LR and its precursor 37 LRP is not completely understood, but previous observations have suggested that 37 LRP can undergo homo- and/or hetero- dimerization with Galectin-3 (Gal-3) to form mature 67 LR. Gal-3 is the only member of the chimera-type group of galectins, and has one C-terminal carbohydrate recognition domain (CRD) that is responsible for binding the ß-galactoside moieties of mono- or oligosaccharides on several host and microbial molecules. The aim of this work was to investigate homo- and hetero-dimerization among the 37 LRP and Gal-3 to form mature 67 LR in mammalian cells using bimolecular fluorescence complementation (BiFC). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=37%20LRP" title="37 LRP">37 LRP</a>, <a href="https://publications.waset.org/abstracts/search?q=67%20LR" title=" 67 LR"> 67 LR</a>, <a href="https://publications.waset.org/abstracts/search?q=Gal-3" title=" Gal-3"> Gal-3</a>, <a href="https://publications.waset.org/abstracts/search?q=BiFC" title=" BiFC"> BiFC</a> </p> <a href="https://publications.waset.org/abstracts/15423/using-of-bimolecular-fluorescence-complementation-bifc-assays-to-study-homo-and-or-heterodimerization-of-laminin-receptor-37-lrp-67-lr-with-galectin-3" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15423.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">504</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">772</span> Role of Imaging in Predicting the Receptor Positivity Status in Lung Adenocarcinoma: A Chapter in Radiogenomics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sonal%20Sethi">Sonal Sethi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mukesh%20Yadav"> Mukesh Yadav</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhimanyu%20Gupta"> Abhimanyu Gupta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The upcoming field of radiogenomics has the potential to upgrade the role of imaging in lung cancer management by noninvasive characterization of tumor histology and genetic microenvironment. Receptor positivity like epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK) genotyping are critical in lung adenocarcinoma for treatment. As conventional identification of receptor positivity is an invasive procedure, we analyzed the features on non-invasive computed tomography (CT), which predicts the receptor positivity in lung adenocarcinoma. Retrospectively, we did a comprehensive study from 77 proven lung adenocarcinoma patients with CT images, EGFR and ALK receptor genotyping, and clinical information. Total 22/77 patients were receptor-positive (15 had only EGFR mutation, 6 had ALK mutation, and 1 had both EGFR and ALK mutation). Various morphological characteristics and metastatic distribution on CT were analyzed along with the clinical information. Univariate and multivariable logistic regression analyses were used. On multivariable logistic regression analysis, we found spiculated margin, lymphangitic spread, air bronchogram, pleural effusion, and distant metastasis had a significant predictive value for receptor mutation status. On univariate analysis, air bronchogram and pleural effusion had significant individual predictive value. Conclusions: Receptor positive lung cancer has characteristic imaging features compared with nonreceptor positive lung adenocarcinoma. Since CT is routinely used in lung cancer diagnosis, we can predict the receptor positivity by a noninvasive technique and would follow a more aggressive algorithm for evaluation of distant metastases as well as for the treatment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lung%20cancer" title="lung cancer">lung cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=multidisciplinary%20cancer%20care" title=" multidisciplinary cancer care"> multidisciplinary cancer care</a>, <a href="https://publications.waset.org/abstracts/search?q=oncologic%20imaging" title=" oncologic imaging"> oncologic imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=radiobiology" title=" radiobiology"> radiobiology</a> </p> <a href="https://publications.waset.org/abstracts/129528/role-of-imaging-in-predicting-the-receptor-positivity-status-in-lung-adenocarcinoma-a-chapter-in-radiogenomics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/129528.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">771</span> Insect Inducible Methanol Production in Plants for Insect Resistance</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gourav%20Jain">Gourav Jain</a>, <a href="https://publications.waset.org/abstracts/search?q=Sameer%20Dixit"> Sameer Dixit</a>, <a href="https://publications.waset.org/abstracts/search?q=Surjeet%20Kumar%20Arya"> Surjeet Kumar Arya</a>, <a href="https://publications.waset.org/abstracts/search?q=Praveen%20C.%20Verma"> Praveen C. Verma</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plant cell wall plays a major role in defence mechanism against biotic and abiotic stress as it constitutes the physical barrier between the microenvironment and internal component of the cell. It is a complex structure composed of mostly carbohydrates among which cellulose and hemicelluloses are most abundant that is embedded in a matrix of pectins and proteins. Multiple enzymes have been reported which plays a vital role in cell wall modification, Pectin Methylesterase (PME) is one of them which catalyses the demethylesterification of homogalacturonans component of pectin which releases acidic pectin and methanol. As emitted methanol is toxic to the insect pest, we use PME gene for the better methanol production. In the current study we showed overexpression of PME gene isolated from Withania somnifera under the insect inducible promoter causes enhancement of methanol production at the time of insect feeds to plants, and that provides better insect resistance property. We found that the 85-90% mortality causes by transgenic tobacco in both chewing (Spodoptera litura larvae and Helicoverpa armigera) and sap-sucking (Aphid, mealybug, and whitefly) pest. The methanol content and emission level were also enhanced by 10-15 folds at different inducible time point interval (15min, 30min, 45min, 60min) which would be analysed by Purpald/Alcohol Oxidase method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=methanol" title="methanol">methanol</a>, <a href="https://publications.waset.org/abstracts/search?q=Pectin%20methylesterase" title=" Pectin methylesterase"> Pectin methylesterase</a>, <a href="https://publications.waset.org/abstracts/search?q=inducible%20promoters" title=" inducible promoters"> inducible promoters</a>, <a href="https://publications.waset.org/abstracts/search?q=Purpald%2FAlcohol%20oxidase" title=" Purpald/Alcohol oxidase"> Purpald/Alcohol oxidase</a> </p> <a href="https://publications.waset.org/abstracts/67908/insect-inducible-methanol-production-in-plants-for-insect-resistance" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67908.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">244</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">770</span> Diversity of Insect Pests of Paddy in Panhala Tehasil, Kolhapur, Maharashtra, India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Manjiri%20A.%20More">Manjiri A. More</a>, <a href="https://publications.waset.org/abstracts/search?q=Manisha%20M.%20Bhosale"> Manisha M. Bhosale</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Agriculture is the backbone of Indian economy and India is one of the world’s largest producers of Rice. Today, paddy crop is facing a severe problem of insect pests and is attacked by more than 100 species of insects, among those 20 species cause economic damage. Rice is the staple food of people of panhala tehasil, Kolhapur, Maharashtra, India. During June 2017 to September 2017 efforts were made to study the diversity of insect pests associated with the paddy crop in the study region. The collection and preservation of the specimens were done by following standard procedure and the identification was done with the help standard literature, taxonomic keys, and webography. In all, 6 species were recorded as pests of paddy in which order Lepidoptera was dominant with 2 species, while orders Diptera, Orthoptera, Hemiptera, and Coleoptera were represented by 1 species each respectively. The results of the present investigation will be helpful for formulating control strategies against these paddy pests. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diversity" title="diversity">diversity</a>, <a href="https://publications.waset.org/abstracts/search?q=insect%20pests" title=" insect pests"> insect pests</a>, <a href="https://publications.waset.org/abstracts/search?q=Panhala" title=" Panhala"> Panhala</a>, <a href="https://publications.waset.org/abstracts/search?q=staple" title=" staple"> staple</a> </p> <a href="https://publications.waset.org/abstracts/98200/diversity-of-insect-pests-of-paddy-in-panhala-tehasil-kolhapur-maharashtra-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/98200.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">132</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">769</span> Feasibility Study on the Bioattactants from Pandanus Palm Extracts for Trapping Rice Insect Pests</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pisit%20Poolprasert">Pisit Poolprasert</a>, <a href="https://publications.waset.org/abstracts/search?q=Phakin%20Kubchanan"> Phakin Kubchanan</a>, <a href="https://publications.waset.org/abstracts/search?q=Keerati%20Tanruean"> Keerati Tanruean</a>, <a href="https://publications.waset.org/abstracts/search?q=Wisanu%20Thongchai"> Wisanu Thongchai</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuttasak%20Chammui"> Yuttasak Chammui</a>, <a href="https://publications.waset.org/abstracts/search?q=Wirot%20Likittrakulwong"> Wirot Likittrakulwong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rice insect pests are problems to rice production. Use of chemicals to minimize these problems of insect pests in paddy field can lead to the residue and affect the health of farmers. Therefore, botanical extracts applied for controlling rice serious enemies should be promoted especially use of plant extract as attractants to lure insects. This research aimed to feasibility study of bioattractants from pandanus palm extracts for trapping insect pets using two different trap models, including plastic bottle and yellow sticky traps. Two main growth and development stages of rice, namely tillering and booting stages, were selected and trapped. The results from both trap models revealed that four rice insect species, including Orseolia oryzae (Wood-Mason), Nilaparvata lugens, Recilia dorsalis, and Nephotettix nigropictus from three families (Cecidomyiidae, Cicadellidae and Delphacidae) and two main orders (Diptera and Hemiptera) were exhibited. All rice insect species mentioned could be found from the yellow sticky trap that were higher than in the bottle trap in which only O. oryzae could be only trapped. From this survey, it was indicated that the yellow sticky trap coated with pandanus palm extracts had a promising potential to use as an attractant for the detection of rice paddy insects in the next future. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pandanus%20palm" title="pandanus palm">pandanus palm</a>, <a href="https://publications.waset.org/abstracts/search?q=bioattractant" title=" bioattractant"> bioattractant</a>, <a href="https://publications.waset.org/abstracts/search?q=bottle%20trap" title=" bottle trap"> bottle trap</a>, <a href="https://publications.waset.org/abstracts/search?q=yellow%20sticky%20trap" title=" yellow sticky trap"> yellow sticky trap</a> </p> <a href="https://publications.waset.org/abstracts/160235/feasibility-study-on-the-bioattactants-from-pandanus-palm-extracts-for-trapping-rice-insect-pests" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/160235.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">125</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">768</span> Produced Gas Conversion of Microwave Carbon Receptor Reforming</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Young%20Nam%20Chun">Young Nam Chun</a>, <a href="https://publications.waset.org/abstracts/search?q=Mun%20Sup%20Lim"> Mun Sup Lim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Carbon dioxide and methane, the major components of biomass pyrolysis/gasification gas and biogas, top the list of substances that cause climate change, but they are also among the most important renewable energy sources in modern society. The purpose of this study is to convert carbon dioxide and methane into high-quality energy using char and commercial activated carbon obtained from biomass pyrolysis as a microwave receptor. The methane reforming process produces hydrogen and carbon. This carbon is deposited in the pores of the microwave receptor and lowers catalytic activity, thereby reducing the methane conversion rate. The deposited carbon was removed by carbon gasification due to the supply of carbon dioxide, which solved the problem of microwave receptor inactivity. In particular, the conversion rate remained stable at over 90% when the ratio of carbon dioxide to methane was 1:1. When the reforming results of carbon dioxide and methane were compared after fabricating nickel and iron catalysts using commercial activated carbon as a carrier, the conversion rate was higher in the iron catalyst than in the nickel catalyst and when no catalyst was used.  <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microwave" title="microwave">microwave</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20reforming" title=" gas reforming"> gas reforming</a>, <a href="https://publications.waset.org/abstracts/search?q=greenhouse%20gas" title=" greenhouse gas"> greenhouse gas</a>, <a href="https://publications.waset.org/abstracts/search?q=microwave%20receptor" title=" microwave receptor"> microwave receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=catalyst" title=" catalyst"> catalyst</a> </p> <a href="https://publications.waset.org/abstracts/77831/produced-gas-conversion-of-microwave-carbon-receptor-reforming" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77831.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">379</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">767</span> On the Thermodynamics of Biological Cell Adhesion</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ben%20Nadler">Ben Nadler</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cell adhesion plays a vital role in many cell activities. The motivation to model cell adhesion is to study important biological processes, such as cell spreading, cell aggregation, tissue formation, and cell adhesion, which are very challenging to study by experimental methods alone. This study provides important insight into cell adhesion, which can lead to improve regenerative medicine and tissue formation techniques. In this presentation the biological cells adhesion is mediated by receptors–ligands binding and the diffusivity of the receptor on the cell membrane surface. The ability of receptors to diffuse on the cell membrane surface yields a very unique and complicated adhesion mechanism, which is exclusive to cells. The phospholipid bilayer, which is the main component in the cell membrane, shows fluid-like behavior associated with the molecules’ diffusivity. The biological cell is modeled as a fluid-like membrane with negligible bending stiffness enclosing the cytoplasm fluid. The in-plane mechanical behavior of the cell membrane is assumed to depend only on the area change, which is motivated by the fluidity of the phospholipid bilayer. In addition, the presence of receptors influences on the local mechanical properties of the cell membrane is accounted for by including stress-free area change, which depends on the receptor density. Based on the physical properties of the receptors and ligands the attraction between the receptors and ligands is modeled as a charged-nonpolar which is a noncovalent interaction. Such interaction is a short-range type, which decays fast with distance. The mobility of the receptor on the cell membrane is modeled using the diffusion equation and Fick’s law is used to model the receptor–receptor interactions. The resultant interaction force, which includes receptor–ligand and receptor–receptor interaction, is decomposed into tangential part, which governs the receptor diffusion, and normal part, which governs the cell deformation and adhesion. The formulation of the governing equations and numerical simulations will be presented. Analysis of the adhesion characteristic and properties are discussed. The roles of various thermomechanical properties of the cell, receptors and ligands on the cell adhesion are investigated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cell%20adhesion" title="cell adhesion">cell adhesion</a>, <a href="https://publications.waset.org/abstracts/search?q=cell%20membrane" title=" cell membrane"> cell membrane</a>, <a href="https://publications.waset.org/abstracts/search?q=receptor-ligand%20interaction" title=" receptor-ligand interaction"> receptor-ligand interaction</a>, <a href="https://publications.waset.org/abstracts/search?q=receptor%20diffusion" title=" receptor diffusion"> receptor diffusion</a> </p> <a href="https://publications.waset.org/abstracts/37546/on-the-thermodynamics-of-biological-cell-adhesion" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37546.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">342</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">766</span> Study of Functional Relevant Conformational Mobility of β-2 Adrenoreceptor by Means of Molecular Dynamics Simulation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=G.%20V.%20Novikov">G. V. Novikov</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20S.%20Sivozhelezov"> V. S. Sivozhelezov</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20S.%20Kolesnikov"> S. S. Kolesnikov</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20V.%20Shaitan"> K. V. Shaitan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study reports about the influence of binding of orthosteric ligands as well as point mutations on the conformational dynamics of β-2-adrenoreceptor. Using molecular dynamics simulation we found that there was a little fraction of active states of the receptor in its apo (ligand free) ensemble corresponded to its constitutive activity. Analysis of MD trajectories indicated that such spontaneous activation of the receptor is accompanied by the motion in intracellular part of its alpha-helices. Thus receptor’s constitutive activity directly results from its conformational dynamics. On the other hand the binding of a full agonist resulted in a significant shift of the initial equilibrium towards its active state. Finally, the binding of the inverse agonist stabilized the receptor in its inactive state. It is likely that the binding of inverse agonists might be a universal way of constitutive activity inhibition in vivo. Our results indicate that ligand binding redistribute pre-existing conformational degrees of freedom (in accordance to the Monod-Wyman-Changeux-Model) of the receptor rather than cause induced fit in it. Therefore, the ensemble of biologically relevant receptor conformations is encoded in its spatial structure, and individual conformations from that ensemble might be used by the cell in conformity with the physiological behaviour. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=seven-transmembrane%20receptors" title="seven-transmembrane receptors">seven-transmembrane receptors</a>, <a href="https://publications.waset.org/abstracts/search?q=constitutive%20activity" title=" constitutive activity"> constitutive activity</a>, <a href="https://publications.waset.org/abstracts/search?q=activation" title=" activation"> activation</a>, <a href="https://publications.waset.org/abstracts/search?q=x-ray%20crystallography" title=" x-ray crystallography"> x-ray crystallography</a>, <a href="https://publications.waset.org/abstracts/search?q=principal%20component%20analysis" title=" principal component analysis"> principal component analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20dynamics%20simulation" title=" molecular dynamics simulation"> molecular dynamics simulation</a> </p> <a href="https://publications.waset.org/abstracts/5618/study-of-functional-relevant-conformational-mobility-of-v-2-adrenoreceptor-by-means-of-molecular-dynamics-simulation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5618.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">256</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">765</span> Targeting Trypanosoma brucei Using Antibody Drug Conjugates against the Transferrin Receptor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Camilla%20Trevor">Camilla Trevor</a>, <a href="https://publications.waset.org/abstracts/search?q=Matthew%20K.%20Higgins"> Matthew K. Higgins</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrea%20Gonzalez-Munoz"> Andrea Gonzalez-Munoz</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20Carrington"> Mark Carrington</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Trypanosomiasis is a devastating disease affecting both humans and livestock in sub-Saharan Africa. The diseases are caused by infection with African trypanosomes, protozoa transmitted by tsetse flies. Treatment currently relies on the use of chemotherapeutics with ghastly side effects. Here, we describe the development of effective antibody-drug conjugates that target the T. brucei transferrin receptor. The receptor is essential for trypanosome growth in a mammalian host but there are approximately 12 variants of the transferrin receptor in the genome. Two of the most divergent variants were used to generate recombinant monoclonal immunoglobulin G using phage display and we identified cross-reactive antibodies that bind both variants using phage ELISA, fluorescence resonance energy transfer assays and surface plasmon resonance. Fluorescent antibodies were used to demonstrate uptake into trypanosomes in culture. Toxin-conjugated antibodies were effective at killing trypanosomes at sub-nanomolar concentrations. The approach of using antibody-drug conjugates has proven highly effective. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibody-drug%20conjugates" title="antibody-drug conjugates">antibody-drug conjugates</a>, <a href="https://publications.waset.org/abstracts/search?q=phage%20display" title=" phage display"> phage display</a>, <a href="https://publications.waset.org/abstracts/search?q=transferrin%20receptor" title=" transferrin receptor"> transferrin receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=trypanosomes" title=" trypanosomes"> trypanosomes</a> </p> <a href="https://publications.waset.org/abstracts/99250/targeting-trypanosoma-brucei-using-antibody-drug-conjugates-against-the-transferrin-receptor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99250.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">155</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">764</span> An Unbiased Profiling of Immune Repertoire via Sequencing and Analyzing T-Cell Receptor Genes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yi-Lin%20Chen">Yi-Lin Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Sheng-Jou%20Hung"> Sheng-Jou Hung</a>, <a href="https://publications.waset.org/abstracts/search?q=Tsunglin%20Liu"> Tsunglin Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Adaptive immune system recognizes a wide range of antigens via expressing a large number of structurally distinct T cell and B cell receptor genes. The distinct receptor genes arise from complex rearrangements called V(D)J recombination, and constitute the immune repertoire. A common method of profiling immune repertoire is via amplifying recombined receptor genes using multiple primers and high-throughput sequencing. This multiplex-PCR approach is efficient; however, the resulting repertoire can be distorted because of primer bias. To eliminate primer bias, 5’ RACE is an alternative amplification approach. However, the application of RACE approach is limited by its low efficiency (i.e., the majority of data are non-regular receptor sequences, e.g., containing intronic segments) and lack of the convenient tool for analysis. We propose a computational tool that can correctly identify non-regular receptor sequences in RACE data via aligning receptor sequences against the whole gene instead of only the exon regions as done in all other tools. Using our tool, the remaining regular data allow for an accurate profiling of immune repertoire. In addition, a RACE approach is improved to yield a higher fraction of regular T-cell receptor sequences. Finally, we quantify the degree of primer bias of a multiplex-PCR approach via comparing it to the RACE approach. The results reveal significant differences in frequency of VJ combination by the two approaches. Together, we provide a new experimental and computation pipeline for an unbiased profiling of immune repertoire. As immune repertoire profiling has many applications, e.g., tracing bacterial and viral infection, detection of T cell lymphoma and minimal residual disease, monitoring cancer immunotherapy, etc., our work should benefit scientists who are interested in the applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=immune%20repertoire" title="immune repertoire">immune repertoire</a>, <a href="https://publications.waset.org/abstracts/search?q=T-cell%20receptor" title=" T-cell receptor"> T-cell receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=5%27%20RACE" title=" 5' RACE"> 5' RACE</a>, <a href="https://publications.waset.org/abstracts/search?q=high-throughput%20sequencing" title=" high-throughput sequencing"> high-throughput sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=sequence%20alignment" title=" sequence alignment"> sequence alignment</a> </p> <a href="https://publications.waset.org/abstracts/88972/an-unbiased-profiling-of-immune-repertoire-via-sequencing-and-analyzing-t-cell-receptor-genes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88972.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">194</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">763</span> Correlation of Leptin with Clinico-Pathological Features of Breast Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saad%20Al-Shibli">Saad Al-Shibli</a>, <a href="https://publications.waset.org/abstracts/search?q=Nasser%20Amjad"> Nasser Amjad</a>, <a href="https://publications.waset.org/abstracts/search?q=Muna%20Al%20Kubaisi"> Muna Al Kubaisi</a>, <a href="https://publications.waset.org/abstracts/search?q=Norra%20Harun"> Norra Harun</a>, <a href="https://publications.waset.org/abstracts/search?q=Shaikh%20Mizan"> Shaikh Mizan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leptin is a multifunctional hormone produced mainly by adipocyte. Leptin and its receptor have long been found associated with breast cancer. The main aim of this study is to investigate the correlation between Leptin/Leptin receptor and the clinicopathological features of breast cancer. Blood samples for ELISA, tissue samples from tumors and adjacent breast tissue were taken from 51 women with breast cancer with a control group of 40 women with a negative mammogram. Leptin and Leptin receptor in the tissues were estimated by immunohistochemistry (IHC). They were localized at the subcellular level by immunocytochemistry using transmission electron microscopy (TEM). Our results showed significant difference in serum leptin level between control and the patient group, but no difference between pre and post-operative serum leptin levels in the patient group. By IHC, we found that the majority of the breast cancer cells studied, stained positively for leptin and leptin receptors with co-expression of leptin and its receptors. No significant correlation was found between leptin/leptin receptors expression with the race, menopausal status, lymph node metastasis, estrogen receptor expression, progesterone receptor expression, HER2 expression and tumor size. Majority of the patients with distant metastasis were associated with high leptin and leptin receptor expression. TEM views both Leptin and Leptin receptor were found highly concentrated within and around the nucleus of the cancer breast cells, indicating nucleus is their principal seat of actions while the adjacent breast epithelial cells showed that leptin gold particles are scattered all over the cell with much less than that of the cancerous cells. However, presence of high concentration of leptin does not necessarily prove its over-expression, because it could be internalized from outside by leptin receptor in the cells. In contrast, leptin receptor is definitely over-expressed in the ductal breast cancer cells. We conclude that reducing leptin levels, blocking its downstream tissue specific signal transduction, and/or blocking the upstream leptin receptor pathway might help in prevention and therapy of breast cancer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title="breast cancer">breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=expression" title=" expression"> expression</a>, <a href="https://publications.waset.org/abstracts/search?q=leptin" title=" leptin"> leptin</a>, <a href="https://publications.waset.org/abstracts/search?q=leptin%20receptors" title=" leptin receptors"> leptin receptors</a> </p> <a href="https://publications.waset.org/abstracts/99477/correlation-of-leptin-with-clinico-pathological-features-of-breast-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99477.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">762</span> Records of Lepidopteron Borers (Lepidoptera) on Stored Seeds of Indian Himalayan Conifers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pawan%20Kumar">Pawan Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Pitamber%20Singh%20Negi"> Pitamber Singh Negi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Many of the regeneration failures in conifers are often being attributed to heavy insect attack and pathogens during the period of seed formation and under storage conditions. Conifer berries and seed insects occur throughout the known range of the hosts and also limit the production of seed for nursery stock. On occasion, even entire seed crops are lost due to insect attacks. The berry and seeds of both the species have been found to be infected with insects. Recently, heavy damage to the berry and seeds of Juniper and Chilgoza Pine was observed in the field as well as in stored conditions, leading to reduction in the viability of seeds to germinate. Both the species are under great threat and regeneration of the species is very low. Due to lack of adequate literature, the study on the damage potential of seed insects was urgently required to know the exact status of the insect-pests attacking seeds/berries of both the pine species so as to develop pest management practices against the insect pests attack. As both the species are also under threat and are fighting for survival, so the study is important to develop management practices for the insect-pests of seeds/berries of Juniper and Chilgoza pine so as to evaluate in the nursery, as these species form major vegetation of their distribution zones. A six-year study on the management of insect pests of seeds of Chilgoza revealed that seeds of this species are prone to insect pests mainly borers. During present investigations, it was recorded that cones of are heavily attacked only by <em>Dioryctria abietella </em>(Lepidoptera: Pyralidae) in natural conditions, but seeds which are economically important are heavily infected, (sometimes up to 100% damage was also recorded) by insect borer, <em>Plodia interpunctella</em> (Lepidoptera: Pyralidae) and is recorded for the first time ‘to author’s best knowledge’ infesting the stored Chilgoza seeds. Similarly, Juniper berries and seeds were heavily attacked only by a single borer, <em>Homaloxestis cholopis</em> (Lepidoptera: Lecithoceridae) recorded as a new report in natural habitat as well as in stored conditions. During the present investigation details of insect pest attack on Juniper and Chilgoza pine seeds and berries was observed and suitable management practices were also developed to contain the insect-pests attack. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=borer" title="borer">borer</a>, <a href="https://publications.waset.org/abstracts/search?q=chilgozapine" title=" chilgozapine"> chilgozapine</a>, <a href="https://publications.waset.org/abstracts/search?q=cones" title=" cones"> cones</a>, <a href="https://publications.waset.org/abstracts/search?q=conifer" title=" conifer"> conifer</a>, <a href="https://publications.waset.org/abstracts/search?q=Lepidoptera" title=" Lepidoptera"> Lepidoptera</a>, <a href="https://publications.waset.org/abstracts/search?q=juniper" title=" juniper"> juniper</a>, <a href="https://publications.waset.org/abstracts/search?q=management" title=" management"> management</a>, <a href="https://publications.waset.org/abstracts/search?q=seed" title=" seed"> seed</a> </p> <a href="https://publications.waset.org/abstracts/89887/records-of-lepidopteron-borers-lepidoptera-on-stored-seeds-of-indian-himalayan-conifers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/89887.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">148</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">761</span> The Role of Estradiol-17β and Type IV Collagen on the Regulation and Expression Level Of C-Erbb2 RNA and Protein in SKOV-3 Ovarian Cancer Cell Line </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Merry%20Meryam%20Martgrita">Merry Meryam Martgrita</a>, <a href="https://publications.waset.org/abstracts/search?q=Marselina%20Irasonia%20Tan"> Marselina Irasonia Tan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One of several aggresive cancer is cancer that overexpress c-erbB2 receptor along with the expression of estrogen receptor. Components of extracellular matrix play an important role to increase cancer cells proliferation, migration and invasion. Both components can affect cancer development by regulating the signal transduction pathways in cancer cells. In recent research, SKOV-3 ovarian cancer cell line, that overexpress c-erbB2 receptor was cultured on type IV collagen and treated with estradiol-17β, to reveal the role of both components on RNA and protein level of c-erbB2 receptor. In this research we found a modulation phenomena of increasing and decreasing of c-erbB2 RNA level and a stabilisation phenomena of c-erbB2 protein expression due to estradiol-17β and type IV collagen. It seemed that estradiol-17β has an important role to increase c-erbB2 transcription and the stability of c-erbB2 protein expression. Type IV collagen has an opposite role. It blocked c-erbB2 transcription when it bound to integrin receptor in SKOV-3 cells. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=c-erbB2" title="c-erbB2">c-erbB2</a>, <a href="https://publications.waset.org/abstracts/search?q=estradiol-17%CE%B2" title=" estradiol-17β"> estradiol-17β</a>, <a href="https://publications.waset.org/abstracts/search?q=SKOV-3" title=" SKOV-3"> SKOV-3</a>, <a href="https://publications.waset.org/abstracts/search?q=type%20IV%20collagen" title=" type IV collagen"> type IV collagen</a> </p> <a href="https://publications.waset.org/abstracts/27964/the-role-of-estradiol-17v-and-type-iv-collagen-on-the-regulation-and-expression-level-of-c-erbb2-rna-and-protein-in-skov-3-ovarian-cancer-cell-line" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27964.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">284</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">760</span> Optimization and Evaluation of 177lu-Dotatoc as a Potential Agent for Peptide Receptor Radionuclide Therapy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20Yousefnia">H. Yousefnia</a>, <a href="https://publications.waset.org/abstracts/search?q=MS.%20Mousavi-Daramoroudi"> MS. Mousavi-Daramoroudi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Zolghadri"> S. Zolghadri</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Abbasi-Davani"> F. Abbasi-Davani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> High expression of somatostatin receptors on a wide range of human tumours makes them as potential targets for peptide receptor radionuclide tomography. A series of octreotide analogues were synthesized while [DOTA-DPhe1, Tyr3]octreotide (DOTATOC) indicated advantageous properties in tumour models. In this study, 177Lu-DOTATOC was prepared with the radiochemical purity of higher than 99% in 30 min at the optimized condition. Biological behavior of the complex was studied after intravenous injection into the Syrian rats. Major difference uptake was observed compared to 177LuCl3 solution especially in somatostatin receptor-positive tissues such as pancreas and adrenal. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Biodistribution" title="Biodistribution">Biodistribution</a>, <a href="https://publications.waset.org/abstracts/search?q=177Lu" title=" 177Lu"> 177Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Octreotide" title=" Octreotide"> Octreotide</a>, <a href="https://publications.waset.org/abstracts/search?q=Syrian%20rats" title=" Syrian rats"> Syrian rats</a> </p> <a href="https://publications.waset.org/abstracts/34294/optimization-and-evaluation-of-177lu-dotatoc-as-a-potential-agent-for-peptide-receptor-radionuclide-therapy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/34294.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">448</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">759</span> Insect Outbreaks, Harvesting and Wildfire in Forests: Mathematical Models for Coupling Disturbances</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20C.%20A.%20Leite">M. C. A. Leite</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Chen-Charpentier"> B. Chen-Charpentier</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Agusto"> F. Agusto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A long-term goal of sustainable forest management is a relatively stable source of wood and a stable forest age-class structure has become the goal of many forest management practices. In the absence of disturbances, this forest management goal could easily be achieved. However, in the face of recurring insect outbreaks and other disruptive processes forest planning becomes more difficult, requiring knowledge of the effects on the forest of a wide variety of environmental factors (e.g., habitat heterogeneity, fire size and frequency, harvesting, insect outbreaks, and age distributions). The association between distinct forest disturbances and the potential effect on forest dynamics is a complex matter, particularly when evaluated over time and at large scale, and is not well understood. However, gaining knowledge in this area is crucial for a sustainable forest management. Mathematical modeling is a tool that can be used to broader the understanding in this area. In this talk we will introduce mathematical models formulation incorporating the effect of insect outbreaks either as a single disturbance in the forest population dynamics or coupled with other disturbances: either wildfire or harvesting. The results and ecological insights will be discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=age-structured%20forest%20population" title="age-structured forest population">age-structured forest population</a>, <a href="https://publications.waset.org/abstracts/search?q=disturbances%20interaction" title=" disturbances interaction"> disturbances interaction</a>, <a href="https://publications.waset.org/abstracts/search?q=harvesting%20insects%20outbreak%20dynamics" title=" harvesting insects outbreak dynamics"> harvesting insects outbreak dynamics</a>, <a href="https://publications.waset.org/abstracts/search?q=mathematical%0D%0Amodeling" title=" mathematical modeling"> mathematical modeling</a> </p> <a href="https://publications.waset.org/abstracts/16948/insect-outbreaks-harvesting-and-wildfire-in-forests-mathematical-models-for-coupling-disturbances" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16948.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">525</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">758</span> DNA Based Identification of Insect Vectors for Zoonotic Diseases From District Faisalabad, Pakistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zain%20Ul%20Abdin">Zain Ul Abdin</a>, <a href="https://publications.waset.org/abstracts/search?q=Mirza%20Aizaz%20Asim"> Mirza Aizaz Asim</a>, <a href="https://publications.waset.org/abstracts/search?q=Rao%20Sohail%20Ahmad%20Khan"> Rao Sohail Ahmad Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Luqman%20Amrao"> Luqman Amrao</a>, <a href="https://publications.waset.org/abstracts/search?q=Fiaz%20Hussain"> Fiaz Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Hasooba%20Hira"> Hasooba Hira</a>, <a href="https://publications.waset.org/abstracts/search?q=Saqi%20Kosar%20Abbas"> Saqi Kosar Abbas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The success of Integrated vector management programmes mainly depends on the correct identification of insect vector species involved in vector borne diseases. Based on molecular data the most important insect species involved as vectors for Zoonotic diseases in Pakistan were identified. The precise and accurate identification of such type of organism is only possible through molecular based techniques like “DNA barcoding”. Morphological species identification in insects at any life stage, is very challenging, therefore, DNA barcoding was used as a tool for rapid and accurate species identification in a wide variety of taxa across the globe and parallel studies revealed that DNA barcoding data can be effectively used in resolving taxonomic ambiguities, detection of cryptic diversity, invasion biology, description of new species etc. A comprehensive survey was carried out for the collection of insects (both adult and immature stages) in district Faisalabad, Pakistan and their DNA was extracted and mitochondrial cytochrome oxidase subunit I (COI-59) barcode sequences was used for molecular identification of immature and adult life stage.This preliminary research work opens new frontiers for developing sustainable insect vectors management programmes for saving lives of mankind from fatal diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=zoonotic%20diseases" title="zoonotic diseases">zoonotic diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=cytochrome%20oxidase" title=" cytochrome oxidase"> cytochrome oxidase</a>, <a href="https://publications.waset.org/abstracts/search?q=and%20insect%20vectors" title=" and insect vectors"> and insect vectors</a>, <a href="https://publications.waset.org/abstracts/search?q=CO1" title=" CO1"> CO1</a> </p> <a href="https://publications.waset.org/abstracts/153916/dna-based-identification-of-insect-vectors-for-zoonotic-diseases-from-district-faisalabad-pakistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/153916.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">168</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">757</span> The Transcription Factor HNF4a: A Key Player in Haematological Disorders</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tareg%20Belali">Tareg Belali</a>, <a href="https://publications.waset.org/abstracts/search?q=Mosleh%20Abomughaid"> Mosleh Abomughaid</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhanad%20Alhujaily"> Muhanad Alhujaily</a> </p> <p class="card-text"><strong>Abstract:</strong></p> HNF4a is one of the steroid hormone receptor family of transcription factors with roles in the development of the liver and the regulation of several critical metabolic pathways, such as glycolysis, drug metabolism, and apolipoproteins and blood coagulation. The transcriptional potency of HNF4a is well known due to its involvement in diabetes and other metabolic diseases. However, recently HNF4a has been discovered to be closely associated with several haematological disorders, mainly because of genetic mutations, drugs, and hepatic disorders. We review HNF4a structure and function and its role in haematological disorders. We discuss possible good therapies that are based on targeting HNF4a. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hepatocyte%20nuclear%20factor%204%20alpha" title="hepatocyte nuclear factor 4 alpha">hepatocyte nuclear factor 4 alpha</a>, <a href="https://publications.waset.org/abstracts/search?q=HNF4a%20nuclear%20receptor" title=" HNF4a nuclear receptor"> HNF4a nuclear receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=steroid%20hormone%20receptor%20family%20of%20transcription%20factors" title=" steroid hormone receptor family of transcription factors"> steroid hormone receptor family of transcription factors</a>, <a href="https://publications.waset.org/abstracts/search?q=hematological%20disorders" title=" hematological disorders"> hematological disorders</a> </p> <a href="https://publications.waset.org/abstracts/149357/the-transcription-factor-hnf4a-a-key-player-in-haematological-disorders" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/149357.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">94</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">756</span> Development and Pre-clinical Evaluation of New ⁶⁴Cu-NOTA-Folate Conjugates for PET Imaging of Folate Receptor-Positive Tumors</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Norah%20Al%20Hokbany">Norah Al Hokbany</a>, <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20Al%20Jammaz"> Ibrahim Al Jammaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Basem%20Al%20Otaibi"> Basem Al Otaibi</a>, <a href="https://publications.waset.org/abstracts/search?q=Yousif%20Al%20Malki"> Yousif Al Malki</a>, <a href="https://publications.waset.org/abstracts/search?q=Subhani%20M.%20Okarvi"> Subhani M. Okarvi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: The folate receptor is over-expressed in a wide variety of human tumors. Conjugates of folate have been shown to be selectively taken up by tumor cells via the folate receptor. In an attempt to develop new folate radiotracers with favorable biochemical properties for detecting folate receptor-positive cancers. Methods: we synthesized ⁶⁴Cu-NOTA- and ⁶⁴Cu-NOTAM-folate conjugates using a straightforward and simple one-step reaction. Radiochemical yields were greater than 95% (decay-corrected) with a total synthesis time of less than 20 min. Results: Radiochemical purities were always greater than 98% without high-performance liquid chromatography (HPLC) purification. These synthetic approaches hold considerable promise as a rapid and simple method for ⁶⁴Cu-folate conjugate preparation with high radiochemical yield in a short synthesis time. In vitro tests on the KB cell line showed that significant amounts of the radio conjugates were associated with cell fractions. Bio-distribution studies in nude mice bearing human KB xenografts demonstrated a significant tumor uptake and favorable bio-distribution profile for ⁶⁴Cu-NOTA- and ⁶⁴Cu-NOTAM-folate conjugate. The uptake in the tumors was blocked by the excess injection of folic acid, suggesting a receptor-mediated process. Conclusion: These results demonstrate that the ⁶⁴Cu-NOTAM-folate conjugate may be useful as a molecular probe for the detection and staging of folate receptor-positive cancers, such as ovarian cancer and their metastasis, as well as monitoring tumor response to treatment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=folate" title="folate">folate</a>, <a href="https://publications.waset.org/abstracts/search?q=receptor" title=" receptor"> receptor</a>, <a href="https://publications.waset.org/abstracts/search?q=tumor%20imaging" title=" tumor imaging"> tumor imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=%E2%81%B6%E2%81%B4Cu-NOTA-folate" title=" ⁶⁴Cu-NOTA-folate"> ⁶⁴Cu-NOTA-folate</a>, <a href="https://publications.waset.org/abstracts/search?q=PET" title=" PET"> PET</a> </p> <a href="https://publications.waset.org/abstracts/156848/development-and-pre-clinical-evaluation-of-new-64cu-nota-folate-conjugates-for-pet-imaging-of-folate-receptor-positive-tumors" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156848.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">108</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">755</span> Acute and Chronic Effect of Biopesticide on Infestation of Whitefly Bemisia tabaci (Gennadius) on the Culantro Cultivation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=U.%20Pangnakorn">U. Pangnakorn</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Chuenchooklin"> S. Chuenchooklin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acute and chronic effects of biopesticide from entomopathogenic nematode (<em>Steinernema thailandensis</em> n. sp.), bacteria ISR (<em>Pseudomonas fluorescens</em>), wood vinegar and fermented organic substances from plants: (neem <em>Azadirachta indica</em> + citronella grass <em>Cymbopogon nardus </em>Rendle + bitter bush <em>Chromolaena odorata </em>L<em>.</em>) were tested on culantro (<em>Eryngium foetidum</em> L.). The biopesticide was investigated for infestation reduction of the major insect pest whitefly (<em>Bemisia tabaci</em> (Gennadius)). The experimental plots were located at a farm in Nakhon Sawan Province, Thailand. This study was undertaken during the drought season (late November to May). Effectiveness of the treatment was evaluated in terms of acute and chronic effect. The populations of whitefly were observed and recorded every hour up to 3 hours with insect nets and yellow sticky traps after the treatments were applied for the acute effect. The results showed that bacteria ISR had the highest effectiveness for controlling whitefly infestation on culantro; the whitefly numbers on insect nets were 12.5, 10.0 and 7.5 after 1 hr, 2 hr, and 3 hr, respectively while the whitefly on yellow sticky traps showed 15.0, 10.0 and 10.0 after 1 hr, 2 hr, and 3 hr, respectively. For chronic effect, the whitefly was continuously collected and recorded at weekly intervals; the result showed that treatment of bacteria ISR found the average whitefly numbers only 8.06 and 11.0 on insect nets and sticky traps respectively, followed by treatment of nematode where the average whitefly was 9.87 and 11.43 on the insect nets and sticky traps, respectively. In addition, the minor insect pests were also observed and collected. The biopesticide influenced the reduction number of minor insect pests (red spider mites, beet armyworm, short-horned grasshopper, pygmy locusts, etc.) with only a few found on the culantro cultivation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=whitefly%20%28Bemisia%20tabaci%20Gennadius%29" title="whitefly (Bemisia tabaci Gennadius)">whitefly (Bemisia tabaci Gennadius)</a>, <a href="https://publications.waset.org/abstracts/search?q=culantro%20%28Eryngium%20foetidum%20L.%29" title=" culantro (Eryngium foetidum L.)"> culantro (Eryngium foetidum L.)</a>, <a href="https://publications.waset.org/abstracts/search?q=acute%20and%20chronic%20effect" title=" acute and chronic effect"> acute and chronic effect</a>, <a href="https://publications.waset.org/abstracts/search?q=entomopathogenic%20nematode%20%28Steinernema%20thailandensis%20n.%20sp.%29" title=" entomopathogenic nematode (Steinernema thailandensis n. sp.)"> entomopathogenic nematode (Steinernema thailandensis n. sp.)</a>, <a href="https://publications.waset.org/abstracts/search?q=bacteria%20ISR%20%28Pseudomonas%20fluorescens%29" title=" bacteria ISR (Pseudomonas fluorescens)"> bacteria ISR (Pseudomonas fluorescens)</a> </p> <a href="https://publications.waset.org/abstracts/43237/acute-and-chronic-effect-of-biopesticide-on-infestation-of-whitefly-bemisia-tabaci-gennadius-on-the-culantro-cultivation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43237.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">754</span> Using Baculovirus Expression Vector System to Express Envelop Proteins of Chikungunya Virus in Insect Cells and Mammalian Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tania%20Tzong">Tania Tzong</a>, <a href="https://publications.waset.org/abstracts/search?q=Chao-Yi%20Teng"> Chao-Yi Teng</a>, <a href="https://publications.waset.org/abstracts/search?q=Tzong-Yuan%20Wu"> Tzong-Yuan Wu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Currently, Chikungunya virus (CHIKV) transmitted to humans by Aedes mosquitoes has distributed from Africa to Southeast Asia, South America, and South Europe. However, little is known about the antigenic targets for immunity, and there are no licensed vaccines or specific antiviral treatments for the disease caused by CHIKV. Baculovirus has been recognized as a novel vaccine vector with attractive characteristic features of an optional vaccine delivery vehicle. This approach provides the safety and efficacy of CHIKV vaccine. In this study, bi-cistronic recombinant baculoviruses vAc-CMV-CHIKV26S-Rhir-EGFP and vAc-CMV-pH-CHIKV26S-Lir-EGFP were produced. Both recombinant baculovirus can express EGFP reporter gene in insect cells to facilitate the recombinant virus isolation and purification. Examination of vAc-CMV-CHIKV26S-Rhir-EGFP and vAc-CMV-pH-CHIKV26S-Lir-EGFP showed that this recombinant baculovirus could induce syncytium formation in insect cells. Unexpectedly, the immunofluorescence assay revealed the expression of E1 and E2 of CHIKV structural proteins in insect cells infected by vAc-CMV-CHIKV26S-Rhir-EGFP. This result may imply that the CMV promoter can induce the transcription of CHIKV26S in insect cells. There are also E1 and E2 expression in mammalian cells transduced by vAc-CMV-CHIKV26S-Rhir-EGFP and vAc-CMV-pH-CHIKV26S-Lir-EGFP. The expression of E1 and E2 proteins of insect and mammalian cells was validated again by Western blot analysis. The vector construction with dual tandem promoters, which is polyhedrin and CMV promoter, has higher expression of the E1 and E2 of CHIKV structural proteins than the vector construction with CMV promoter only. Most of the E1 and E2 proteins expressed in mammalian cells were glycosylated. In the future, the expression of structural proteins of CHIKV in mammalian cells is expected can form virus-like particle, so it could be used as a vaccine for chikungunya virus. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chikungunya%20virus" title="chikungunya virus">chikungunya virus</a>, <a href="https://publications.waset.org/abstracts/search?q=virus-like%20particle" title=" virus-like particle"> virus-like particle</a>, <a href="https://publications.waset.org/abstracts/search?q=vaccines" title=" vaccines"> vaccines</a>, <a href="https://publications.waset.org/abstracts/search?q=baculovirus%20expression%20vector%20system" title=" baculovirus expression vector system"> baculovirus expression vector system</a> </p> <a href="https://publications.waset.org/abstracts/16109/using-baculovirus-expression-vector-system-to-express-envelop-proteins-of-chikungunya-virus-in-insect-cells-and-mammalian-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16109.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">423</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=6">6</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=7">7</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=8">8</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=9">9</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=10">10</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=26">26</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=27">27</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=insect%20gustatory%20receptor&page=2" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div 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