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Analysis of up to 18 and sorting of up to 15 fluorescent parameters is available from a wide variety of samples and cell types. Our staff is experienced in experimental and antibody panel design, practical operation of multiple flow cytometric platforms, and basic and advanced data analysis." /> <link rel="canonical" href="https://gladstone.org/science/flow-cytometry-core" /> <link rel="image_src" href="https://gladstone.org/sites/default/files/GladstoneShareImg2.jpg" /> <meta name="twitter:card" content="summary_large_image" /> <meta name="twitter:site" content="@GladstoneInst" /> <meta name="twitter:description" content="The Gladstone Flow Cytometry Core enables scientists to analyze or isolate cells based on their phenotypic or functional properties, and can be used in conjunction with other core technologies to form a robust and effective pipeline for cellular analysis. Analysis of up to 18 and sorting of up to 15 fluorescent parameters is available from a wide variety of samples and cell types. Our staff is experienced in experimental and antibody panel design, practical operation of multiple flow cytometric platforms, and basic and advanced data analysis." /> <meta name="twitter:title" content="Flow Cytometry Core" /> <meta name="twitter:image" content="https://gladstone.org/sites/default/files/GladstoneShareImg2.jpg" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" id="viewport" content="width=device-width, initial-scale=1, user-scalable=yes" /> <link rel="icon" href="/sites/default/files/Social-Media_0.png" type="image/png" /> <title>Flow Cytometry Core | Gladstone Institutes</title> <link rel="stylesheet" media="all" href="/modules/contrib/ajax_loader/css/throbber-general.css?sndln6" /> <link rel="stylesheet" media="all" href="/libraries/drupal-superfish/css/superfish.css?sndln6" /> <link rel="stylesheet" media="all" 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data-drupal-messages-fallback class="hidden"></div> <div id="block-showcase-lite-page-title" class="clearfix block block-core block-page-title-block"> <div class="content"> <h1 class="title page-title"><span class="field field--name-title field--type-string field--label-hidden">Flow Cytometry Core</span> </h1> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="clearfix main-content region--dark-typography region--white-background region--no-separator"> <div class="landing-page-container" > <div class="clearfix main-content__container"> <div class="row"> <section> <div class="clearfix main-content__section" > <div class="region region-content"> <div id="block-showcase-lite-content" class="clearfix block block-system block-system-main-block"> <div class="content"> <article data-history-node-id="3270" class="node node--type-core-mini-sites node--promoted node--view-mode-full clearfix"> <div class="node__container"> <div class="node__main-content clearfix"> <header class="node__header"> </header> <div id="mini-core-tabs" class="row"> <div id="subtabs" class="subtabs"> <ul class="sudonav"> <li class="tab"><a href="#overview"><span>Overview</span></a></li> <li class="tab"><a href="#services"><span>Services</span></a></li> <li class="tab"><a href="#equipment"><span>Equipment</span></a></li> <li class="tab"><a href="#fees-and-scheduling"><span>Fees and Scheduling</span></a></li> <li class="tab"><a href="#publications"><span>Publications</span></a></li> <li class="tab"><a href="#training"><span>Training</span></a></li> <li class="tab"><a href="#faqs"><span>FAQs</span></a></li> </ul> <div class="content-wrap"> <section id="overview"> <div id="intro"> <div class="container"> <p class="large">The Gladstone Flow Cytometry Core enables scientists to analyze or isolate cells based on their phenotypic or functional properties, and can be used in conjunction with other core technologies to form a robust and effective pipeline for cellular analysis. Analysis of up to 18 and sorting of up to 15 fluorescent parameters is available from a wide variety of samples and cell types. Our staff is experienced in experimental and antibody panel design, practical operation of multiple flow cytometric platforms, and basic and advanced data analysis.</p> <p><a href="#services">Discover how our core can help you with your flow cytometry needs.</a></p> </div> </div> <div id="manager"> <div class="container"> <h2>Contact</h2> <div class="col-md-6"> <div class="field field--name-field-core-contact field--type-entity-reference field--label-hidden field--entity-reference-target-type-user clearfix field__item"><article class="user user--coredirector"> <div class="user__compact"> <p> <b>Jane Srivastava</b><br> Core Director <br> <a class="cracked" href="mailto:jane.srivastava@gladstone.ucsf.edu">Email</a> <a class="forflow" href="mailto:flow-core@gladstone.ucsf.edu">Email</a><br> </p> </div> <style> .forflow {display:none} </style> </article> </div> </div> <div class="col-md-6"> <div class="field field--name-field-contact2 field--type-entity-reference field--label-hidden field--entity-reference-target-type-user clearfix field__item"><article class="user user--coredirector"> <div class="user__compact"> <p> <b>Kevin Pastores, MS </b><br> Research Technologist II <br> <a class="cracked" href="mailto:kevin.pastores@gladstone.ucsf.edu">Email</a> <a class="forflow" href="mailto:flow-core@gladstone.ucsf.edu">Email</a><br> </p> </div> <style> .forflow {display:none} </style> </article> </div> </div> </div> </div> <div class="core-members"> <div class="container"> <h2>Core Members</h2> <div class="views-element-container"><div class="view view-user-admin-people view-id-user_admin_people view-display-id-core_members js-view-dom-id-33ec2dab6431b3fb4577e030d503bdc8727edf22050f33336392b08f9cd23701"> <div class="view-content"> <div class="views-view-grid horizontal cols-2 clearfix"> <div class="views-row clearfix row-1"> <div class="views-col col-1" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Kevin Pastores</b><br>Research Technologist II<br></p> </div> </article> </div> <div class="views-col col-2" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Jane Srivastava</b><br>Core Director, Flow Cytometry, Light Microscopy and Histology<br></p> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> <div class="corebk1">   </div> <div id="related-news"> <div class="container"> <h2>Related News</h2> <div class="owl-carousel" id="news-carousel"> <a href="/news/biomedical-research-flows-new-directions" title="Read more" target="_self"><div class="item"><img alt="Jane Srivastava, director of the Flow Cytometry Core at Gladstone Institutes" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/gladstone-flowcytometry-hero.jpg?itok=ZfC-RKLS " /><div class="blowpic">Biomedical Research That “Flows” in New Directions<br><div class="summy">Improvements in the cell analysis technique known as flow cytometry are helping Gladstone researchers make discoveries in immunology, stem cell biology, cardiology, and neuroscience</div><div class="authored"><time datetime="2022-12-20T09:11:40-08:00" class="datetime">December 20, 2022</time> </div></div></div></a><a href="/news/meet-gladstone-jane-srivastava" title="Read more" target="_self"><div class="item"><img alt="Jane Srivastava animated gif" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/Meet-Gladstone-Jane-Srivastava-Hero.gif?itok=jFt2OmNd " /><div class="blowpic">Meet Gladstone: Jane Srivastava<br><div class="summy">Jane Srivastava, director of Gladstone's Flow Cytometry Core, shares how her interest in science started at a young age and how lasers can be used for more than defeating stormtroopers</div><div class="authored"><time datetime="2021-10-19T00:00:00-07:00" class="datetime">October 19, 2021</time> </div></div></div></a><a href="/news/herpesviruses-hedge-their-bets-optimize-survival" title="Read more" target="_self"><div class="item"><img alt="Sonali Chaturvedi and Leor Weinberger in masks" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/leor-weinberger-sonali-chaturvedi-06-20.jpg?itok=omG8_Rj_ " /><div class="blowpic">Herpesviruses Hedge Their Bets to Optimize Survival<br><div class="summy">Varying levels of proteins in cytomegalovirus particles help the virus survive in different conditions.</div><div class="authored"><time datetime="2020-07-06T05:01:00-07:00" class="datetime">July 6, 2020</time> </div></div></div></a><a href="/news/newly-discovered-links-between-hiv-and-cell-stress-could-point-novel-treatments" title="Read more" target="_self"><div class="item"><img alt="Melanie Ott and Albert Vallejo-Gracia in the lab" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/albert-vallejo-gracia_melanie-ott.jpg?itok=5l7ADk52 " /><div class="blowpic">Newly Discovered Links between HIV and Cell Stress Could Point to Novel Treatments<br><div class="summy">HIV establishes latency by co-opting a cellular stress pathway</div><div class="authored"><time datetime="2020-06-14T17:00:00-07:00" class="datetime">June 14, 2020</time> </div></div></div></a><a href="/news/which-cells-does-hiv-prefer-infect" title="Read more" target="_self"><div class="item"><img alt="Marielle Cavrois and Nadia Roan" class="owl-lazy" data-src="/sites/default/files/styles/news_card/public/news-events/RoanCavrois_201707_GladstoneInstitutes_0.jpg?itok=Fz7CUq_T " /><div class="blowpic">Which Cells Does HIV Prefer to Infect?<br><div class="summy">Researchers created an atlas to identify cells most likely to be infected</div><div class="authored"><time datetime="2017-07-25T13:50:00-07:00" class="datetime">July 25, 2017</time> </div></div></div></a> </div> </div> </div> </section> <section id="services"> <div id="capab-intro"> <div class="container"> <h2 class="tabtop">Services Provided</h2> <div class="alternator"> <div class="row"> <div class="col-sm-6"> <h3>Cell Sorting</h3> <p>Cell sorting for up to 18 fluorescent parameters with the following specifications:</p> <ul> <li>5 available lasers: UV (355nm), Violet (405nm), Blue (488nm), Yellow-Green (561nm), Red (640nm).</li> <li>Functional marker sorting and analysis (e.g., phospho-flow, cell cycle, and mitochondrial assays).</li> <li>Single-cell sorting into 96-well plates for cloning or further sequencing downstream analysis.</li> </ul> <p>Sorting into the following receptacles:</p> <ul> <li>1.5ml Eppendorf tubes</li> <li>5ml Falcon tubes</li> <li>15ml Falcon tubes</li> <li>Any well plate (12, 24, 48, 96 and 384)</li> <li>Terasaki plate</li> <li>Standard/frosted end slide</li> </ul> <p>Aseptic sorting is available.</p> </div> <div class="col-sm-6"><img alt="Cell Sorting graphic" data-entity-type data-entity-uuid height="466" src="/sites/default/files/inline-images/cell-sorting1.png" width="485" loading="lazy"> <p> <img alt="Cell sorting 2" data-entity-type data-entity-uuid height="456" src="/sites/default/files/inline-images/cell-sorting2.png" width="472" loading="lazy"></p> <p class="text-align-center small" style="margin-top:14px;">Purification of GFP positive cells using the FACSAria Fusion.</p> </div> </div> <div class="row"> <div class="col-sm-6"><img alt="Cell Analyzer" data-entity-type data-entity-uuid height="493" src="/sites/default/files/inline-images/cell-analyzers.png" width="502" loading="lazy"> <p class="text-align-center small" style="margin-top:14px;">Detection of mouse mesenchymal PaS cells.</p> </div> <div class="col-sm-6"> <h3>Cell Analyzers</h3> <ul> <li>Analysis of up to 40 fluorescences in one tube.</li> <li>Analysis of extra, intracellular and functional markers as well as fluorescent proteins.</li> <li>High-throughput analysis from 96- and 384-well plates (up to 18 fluorescent parameters) for all analyzers.</li> </ul> </div> </div> <div class="row"> <div class="col-sm-6"> <h3>Imaging Cytometry</h3> <ul> <li>Imaging Cytometry for up to 6 fluorescences, or 4 fluorescences including brightfield and side scatter.</li> <li>20X, 40X and 60X magnification available.</li> </ul> </div> <div class="col-sm-6" style="text-align:center;"><img alt="Imaging Cytometry" data-entity-type data-entity-uuid height="452" src="/sites/default/files/inline-images/imaging-cytometry.png" width="389" loading="lazy"> <p class="text-align-center small" style="margin-top:14px;">Detection of mCherry positive bacteria in cells using the Imagestream.</p> </div> </div> <div class="row"> <div class="col-sm-6"><img alt="Plluripotency Testing" data-entity-type data-entity-uuid height="452" src="/sites/default/files/inline-images/PluripotencyTesting.png" width="449" loading="lazy"> <p class="text-align-center small" style="margin-top:14px;">Detection of pluripotency markers Tra-1-60 and Tra-1-81 in human induced pluripotent stem cells.</p> </div> <div class="col-sm-6"> <h3>hiPSC Pluripotency Testing</h3> <p>Test whether the human induced pluripotent stem cells (hiPS cells) that you have received or grown express a panel of pluripotent markers and define their pluripotency status. Markers include Tra-1-60, Tra-1-81, SSEA-3, SSEA-4, CD30, CDH3, Nanog, Oct 3_4 and Sox-2, which you can choose from to run in your panel. Turnaround time is approximately 1 day and you’ll receive all raw data files at the conclusion of the experiment.</p> </div> </div> <div class="row"> <div class="col-sm-6"> <h3>Additional Services</h3> <p>Gladstone’s Flow Cytometry Core also offers sample processing, including staining and running of experimental samples. Data analysis services are also available.</p> </div> <div class="col-sm-6"><img alt="Additional Services" data-entity-type data-entity-uuid height="364" src="/sites/default/files/inline-images/AdditionalServices.png" width="370" loading="lazy"> <p class="text-align-center small" style="margin-top:14px;">Detection of Sca-1/c-Kit positive markers in mouse hematopoietic stem cells.</p> </div> </div> </div> <style type="text/css">.alternator .row {margin-top:48px} .alternator img {width:100%;max-width:100%} </style> </div></div> <div class="corebk2"> </div> </section> <section id="fees-and-scheduling"> <div class="container"> <h2 class="tabtop">Fees and Scheduling</h2> <p>For more information on our fees, <a href="mailto:flow-core@gladstone.ucsf.edu">contact the core.</a></p> </div> </section> <section id="publications"> <div class="container"> <h2 class="tabtop">Publications</h2> <p><a href="https://www.pnas.org/content/117/29/17240/tab-article-info">A molecular mechanism for probabilistic bet hedging and its role in viral latency.</a> Sonali Chaturvedi, Jonathan Klein, Noam Vardi, Cynthia Bolovan-Fritts, Marie Wolf, Kelvin Du, Luwanika Mlera, Meredith Calvert, Nathaniel J. Moorman, Felicia Goodrum, Bo Huang, Leor S. Weinberger.</p> <p><a href="https://elifesciences.org/articles/55487 ">HIV efficiently infects T cells from the endometrium and remodels them to promote systemic viral spread.</a> Tongcui Ma, Xiaoyu Luo, Ashley F George, Gourab Mukherjee, Nandini Sen, Trimble L Spitzer, Linda C Giudice, Warner C Greene, Nadia R Roan.</p> <p><a href="https://www.nature.com/articles/s41556-020-00579-5?elqTrackId=91fe34a31a8e4d95a9231f8bf2200ced#Ack1 ">SIRT1 is downregulated by autophagy in senescence and ageing.</a> Caiyue Xu, Lu Wang, Parinaz Fozouni, Gry Evjen, Vemika Chandra, Jing Jiang, Congcong Lu, Michael Nicastri, Corey Bretz, Jeffrey D. Winkler, Ravi Amaravadi, Benjamin A. Garcia, Peter D. Adams, Melanie Ott, Wei Tong, Terje Johansen, Zhixun Dou, Shelley L. Berger.</p> <p><a href="https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.a.24262?casa_token=Hl02ZfRDZhAAAAAA%3AzDlFkJDHtB0niPALE_t4yad-sdPbICPl-clCt6SzGSdV-m15ICdWBiXDEF0lXS4rLv7_Vq2xRL7iQw">Remote Training of SRL Users and Staff in a Global Pandemic.</a> Kathleen Daniels, Alexis Conway, Rui Gardner, Lola Martinez, Kylie M. Price, Sarah Schneider, Rachael Sheridan, Jane Srivastava, Sherry Thornton.</p> <p><a href="https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.24261">Shared Resource Laboratory Operations: Changes Made During Initial Global COVID‐19 Lockdown of 2020.</a> Jessica B. Back, Cora H. Chadick, Juan J. Garcia Vallejo, Eva Orlowski‐Oliver, Radhika Patel, Caroline E. Roe, Jane Srivastava, Rachael V. Walker.</p> <p><a href="https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3701636">Transcription Factor Overexpression Drives Reliable Differentiation of Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells.</a> Tessa E. Dewell, Ketrin Gjoni, Angela Z. Liu, Ashley R.G. Libby, Anthony T. Moore, Po-Lin So, Bruce R. Conklin.</p> <p><a href="https://jvi.asm.org/content/early/2020/10/22/JVI.01331-20">Shared Mechanisms Govern HIV Transcriptional Suppression in Circulating CD103+ and Gut CD4+ T Cells. </a>Steven A. Yukl, Shahzada Khan, Tsui-Hua Chen, Martin Trapecar, Frank Wu, Guorui Xie, Sushama Telwatte, Daniel Fulop, Alexander R. Pico, Gregory M. Laird, Kristen D. Ritter, Norman G. Jones, Chuanyi M. Lu, Robert F. Siliciano, Nadia R. Roan, Jeffrey M. Milush, Ma Somsouk, Steven G. Deeks, Peter W. Hunt, Shomyseh Sanjabi.</p> <p><a href="https://www.biorxiv.org/content/10.1101/2020.04.23.058123v1.full.pdf">FOXO1 promotes HIV Latency by suppressing ER stress in T cells.</a> Albert Vallejo-Gracia, Irene P. Chen, Rosalba Perrone, Emilie Besnard, Daniela Boehm, Emilie Battivelli, Tugsan Tezil, Karsten Krey, Kyle A. Raymond, Philip A. Hull, Marius Walter, Ireneusz Habrylo, Andrew Cruz, Steven Deeks, Satish Pillai, Eric Verdin, Melanie Ott.</p> <p><a href="https://www.biorxiv.org/content/10.1101/2020.11.01.363713v1.full.pdf+html">Silencing of E-cadherin in induced human pluripotent stem cells promotes extraembryonic fates accompanying multilineage differentiation.</a> Ashley RG Libby, Ivana Vasic, David A Joy, Martina Z Krakora, Fredrico N Mendoza-Camacho, Bruce R Conklin, Todd C McDevitt.</p> <p><a href="https://www.sciencedirect.com/science/article/pii/S2211124720309438">In Vivo Chimeric Alzheimer’s Disease Modeling of Apolipoprotein E4 Toxicity in Human Neurons.</a> Ramsey Najm, Kelly A.Zalocusky, Misha Zilberter, Seo Yeon Yoon, Yanxia Hao, Nicole Koutsodendris, MaxineNelson, Antara Rao, Alice Taubes, Emily A. Jones, Yadong Huang.</p> <p class="cntrbutt"><a class="morepub button outline">More Publications</a></p> </div> <div class="corebk3"> </div> </section> <section id="training"> <div class="container"> <h2 class="tabtop">Training</h2> <h3>How Do I Get Trained to Use the Equipment in the Gladstone Flow Cytometry Core?</h3> <p>Contact the flow core staff to start the practical training process.</p> <p>To get started, you’ll need to:</p> <ul> <li><a href="https://gladstone.corefacilities.org/account/34/signup" rel="noopener" target="_blank">Register for an iLab account.</a></li> <li>Read, sign, and return the signature page of the <a href="https://padlet.com/janesrivastava5/Bookmarks/wish/2400933340" rel="noopener" target="_blank">general regulations document</a> to the core <a href="mailto:flow-core@gladstone.ucsf.edu">by email</a>.</li> <li>Complete Gladstone’s <a href="https://forms.gle/eWvAiBMTuY4AfCt17" rel="noopener" target="_blank">Flow Core Intro Questionnaire.</a></li> </ul> <p>You can also refer to <a href="https://padlet.com/janesrivastava5/gladstone-flow-cytometry-core-talks-papers-and-online-resour-3t6ajihizxq2cpvy" target="_blank">additional resources</a> to learn more about flow cytometry.</p> </div> <div class="corebk4"> </div> </section> <section id="equipment"> <div id="equip-intro" class="container"> <p class="large">Support your research needs with our cutting-edge equipment and expertise.</p> </div> <div id="equip-list"> <div class="views-element-container"><div class="view view-equipment view-id-equipment view-display-id-flow_cytometry js-view-dom-id-8e58c30e171199c73af218a992635182155879dcd5aa9a83988acc50f7bd0416"> <div class="view-header"> <style> .view-display-id-confocal #3040 {visibility:hidden !important} </style> </div> <div class="view-content"> <div class="flow- views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2024-07/nanofcm-analyzer.jpg" /> </div> <div class="flowtext"> <h4>NanoFCM Analyzer</h4> <p>The NanoFCM Analyzer can be used for the multiparameter characterization, sizing and concentration of natural and synthetic nanoparticles (7-1000 nm) at the single-particle level, including detection of extracellular vesicles, mitochondria, bacteria, viruses, nanomedicine, nanomaterial and other nanoparticles.</p> <p>Specifications:</p> <ul> <li>Laser (blue, green, red)</li> <li>One side-scatter channel</li> <li>Two fluorescence channels</li> <li>Up to 12,000 particles/min</li> <li>Lower detection limit 7 nm (gold nanoparticles) to 40 nm (exosomes, viruses)</li> <li>High detection limit (1 micron)</li> </ul> <p>Practical and theoretical training is available for the Nano FCM, as well as experimental and data analysis support.</p> <p id="manu-butt"><a target="_blank" href="https://www.nanofcm.com/flow-nanoanalyzer/" class="button navy">Manufacturer Site</a></p> </div> </div> </div></div></div> <div class="flow-5728 views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2021-10/fortessa-x-20.jpg" /> </div> <div class="flowtext"> <h4>Fortessa X-20</h4> <p>The Fortessa X-20 cell analyzer delivers high-performance, multicolor analysis and is configured with five lasers to detect up to 20 parameters (18 fluorescent and 2 scatter) simultaneously. An integrated High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from 96- and 384-well microtiter plates.</p> <p>Practical and theoretical training is available for the Fortessa X-20, as well as experimental and data analysis support.</p> <div id="fortessa-config"> <table> <tbody> <tr> <th>LASER</th> <th>CHANNEL</th> <th>BANDPASS FILTER</th> <th>LONGPASS MIRROR</th> </tr> <tr> <td rowspan="3">UV (355 nm)</td> <td>BUV737</td> <td>740/35</td> <td>690LP</td> </tr> <tr> <td>BUV496</td> <td>515/30</td> <td>450LP</td> </tr> <tr> <td>BUV396</td> <td>379/28</td> <td> </td> </tr> <tr> <td rowspan="6">Violet (405 nm)</td> <td>BV786</td> <td>780/60</td> <td>750LP</td> </tr> <tr> <td>BV711</td> <td>710/50</td> <td>690LP</td> </tr> <tr> <td>BV650</td> <td>670/30</td> <td>635LP</td> </tr> <tr> <td>BV605</td> <td>610/20</td> <td>600LP</td> </tr> <tr> <td>BV510/AMCYAN</td> <td>470/15</td> <td>450LP</td> </tr> <tr> <td>BV421/BFP/DAPI</td> <td>431/28</td> <td>410LP</td> </tr> <tr> <td rowspan="4">Blue (488 nm)</td> <td>Per-CP-Cy5.5</td> <td>695/40</td> <td>690LP</td> </tr> <tr> <td>FITC, BB515</td> <td>530/30</td> <td>505LP</td> </tr> <tr> <td>SSC</td> <td>488/10</td> <td> </td> </tr> <tr> <td>FSC</td> <td> </td> <td> </td> </tr> <tr> <td rowspan="4">Yellow/Green (561 nm)</td> <td>PE-Cy7</td> <td>780/60</td> <td>750LP</td> </tr> <tr> <td>PE-Cy5</td> <td>670/30</td> <td>650LP</td> </tr> <tr> <td>PE-CF594</td> <td>610/20</td> <td>600LP</td> </tr> <tr> <td>PE</td> <td>586/15</td> <td>570LP</td> </tr> <tr> <td rowspan="3">Red (637 nm)</td> <td>APC-CY7</td> <td>780/60</td> <td>750LP</td> </tr> <tr> <td>AF-700</td> <td>710/50</td> <td>690LP</td> </tr> <tr> <td>APC</td> <td>670/30</td> <td>650LP</td> </tr> </tbody> </table> </div> <p id="manu-butt"><a target="_blank" href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa-x-20" class="button navy">Manufacturer Site</a></p> </div> </div> </div></div></div> <div class="flow-7270 views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2022-03/CytekAurora.jpeg" /> </div> <div class="flowtext"> <h4>Cytek Aurora</h4> <p>The Cytek Aurora is a spectral cytometry analyzer configured with four lasers (violet, blue, yellow/green, and red) to detect up to 48 parameters simultaneously. Other features include autofluorescence extraction, ability to determine fluorescences in one tube that are unable to be distinguished by regular flow cytometry, and intuitive software with transferable experimental workspaces.</p> <p>Practical and theoretical training is available for the Aurora, as well as experimental and data analysis support.</p> <p>Aurora configuration:</p> <ul> <li>16 violet parameters</li> <li>14 blue parameters</li> <li>10 yellow/green parameters</li> <li>8 red parameters</li> </ul> <p id="manu-butt"><a target="_blank" href="https://cytekbio.com/pages/aurora" class="button navy">Manufacturer Site</a></p> </div> </div> </div></div></div> <div class="flow-6129 views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2021-10/bd-facsaria-fusions.jpg" /> </div> <div class="flowtext"> <h4>BD FACSAria Fusion Cell Sorter</h4> <p>Two BD FACSAria Fusions cell sorters are configured to sort up to 20 parameters (18 fluorescence and 2 scatter). A choice of nozzles allows users sort a wide range of particle sizes. Nozzles are available in four sizes: 70, 85, 100, and 130 microns. Bulk, single cell and index sorting are available.</p> <p>Practical and theoretical training is available for the Fusion, as well as experimental and data analysis support.</p> <p id="fusionequipbuttons"><a class="button outline" id="fusion-config-button">Configuration</a> <a class="button magenta" id="fusion-vessels-button">Collection Vessels</a></p> <div id="fusion-vessels"> <table> <tbody> <tr> <th>TUBES</th> <th>PLATES</th> <th>SLIDES</th> </tr> <tr> <td width="33%">5 ml Falcon</td> <td width="33%">6 well</td> <td width="33%">Standard</td> </tr> <tr> <td>15 ml Falcon</td> <td>12 well</td> <td>Frosted End</td> </tr> <tr> <td rowspan="5">Eppendorf</td> <td>24 well</td> <td> </td> </tr> <tr> <td>48 well</td> <td> </td> </tr> <tr> <td>96 well</td> <td> </td> </tr> <tr> <td>384 well</td> <td> </td> </tr> <tr> <td>Terasaki</td> <td> </td> </tr> </tbody> </table> </div> <div id="fusion-config"> <table> <tbody> <tr> <th>LASER</th> <th>CHANNEL</th> <th>BANDPASS FILTER</th> <th>LONGPASS MIRROR</th> </tr> <tr> <td rowspan="3" width="30%">UV (355 nm)</td> <td width="30%">BUV737</td> <td width="20%">730/45</td> <td width="20%">690LP</td> </tr> <tr> <td>BUV496</td> <td>515/30</td> <td>450LP</td> </tr> <tr> <td>BUV396</td> <td>379/28</td> <td> </td> </tr> <tr> <td rowspan="6">Violet (402 nm)</td> <td>BV786</td> <td>780/60</td> <td>750LP</td> </tr> <tr> <td>BV711</td> <td>670/30</td> <td>690LP</td> </tr> <tr> <td>BV650</td> <td>670/30</td> <td>635LP</td> </tr> <tr> <td>BV605</td> <td>610/20</td> <td>600LP</td> </tr> <tr> <td>BV480</td> <td>470/14</td> <td>450LP</td> </tr> <tr> <td>BV421</td> <td>431/28</td> <td>410LP</td> </tr> <tr> <td rowspan="4">Blue (488 nm)</td> <td>Per-CP-Cy5.5</td> <td>710/50</td> <td>690LP</td> </tr> <tr> <td>FITC</td> <td>530/30</td> <td>502LP</td> </tr> <tr> <td>SSC</td> <td>488/10</td> <td> </td> </tr> <tr> <td>FSC</td> <td> </td> <td> </td> </tr> <tr> <td rowspan="4">Yellow/Green (561 nm)</td> <td>PE-Cy7</td> <td>780/60</td> <td>750LP</td> </tr> <tr> <td>PE-Cy5</td> <td>670/30</td> <td>650LP</td> </tr> <tr> <td>PE-CF594</td> <td>610/20</td> <td>600LP</td> </tr> <tr> <td>PE</td> <td>586/15</td> <td>570LP</td> </tr> <tr> <td rowspan="3">Red (637 nm)</td> <td>APC-CY7</td> <td>780/60</td> <td>750LP</td> </tr> <tr> <td>AF-700</td> <td>710/50</td> <td>690LP</td> </tr> <tr> <td>APC</td> <td>670/30</td> <td>650LP</td> </tr> </tbody> </table> </div> <p id="manu-butt"><a target="_blank" href="https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-sorters/bd-facsaria-fusion" class="button navy">Manufacturer Site</a></p> </div> </div> </div></div></div> <div class="flow-5727 views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2021-10/attunenxt.jpg" /> </div> <div class="flowtext"> <h4>Attune NxT Flow Cytometer</h4> <p>The Attune NxT Flow Cytometer is a compact, benchtop cell analyzer that has been configured with four lasers to run and analyze multicolor panels of up to 16 colors (14 fluorescence and 2 scatter). An integrated autosampler provides rapid, fully automated sample acquisition from 96- and 384-well microtiter plates.</p> <p>Practical and theoretical training is available for the Attune, as well as experimental and data analysis support.</p> <div id="attuneequipbuttons"> <table> <tbody> <tr> <th>LSER</th> <th>CHANNEL</th> <th>BANDPASS FILTER</th> <th>LONGPASS FILTER</th> </tr> <tr> <td rowspan="6" width="30%">Violet (405 nm)</td> <td width="30%">VL1</td> <td width="20%">450/40</td> <td width="20%">417LP</td> </tr> <tr> <td>VL2</td> <td>525/50</td> <td>495LP</td> </tr> <tr> <td>VL3</td> <td>610/20</td> <td>555LP</td> </tr> <tr> <td>VL4</td> <td>660/20</td> <td>635LP</td> </tr> <tr> <td>VL5</td> <td>710/50</td> <td>680LP</td> </tr> <tr> <td>VL6</td> <td>780/60</td> <td>740LP</td> </tr> <tr> <td rowspan="3">Blue (488 nm)</td> <td>BL1</td> <td>530/30</td> <td>495LP</td> </tr> <tr> <td>BL2</td> <td>695/40</td> <td>555LP</td> </tr> <tr> <td>SSC</td> <td> </td> <td> </td> </tr> <tr> <td rowspan="3">Yellow/Green (561 nm)</td> <td>YL1</td> <td>585/16</td> <td>577LP</td> </tr> <tr> <td>YL2</td> <td>620/15</td> <td>600LP</td> </tr> <tr> <td>YL3</td> <td>780/60</td> <td>650LP</td> </tr> <tr> <td rowspan="3">Red (637 nm)</td> <td>RL1</td> <td>670/14</td> <td>654LP</td> </tr> <tr> <td>RL2</td> <td>720/30</td> <td>690LP</td> </tr> <tr> <td>RL3</td> <td>780/60</td> <td>4740LP</td> </tr> </tbody> </table> </div> <p id="manu-butt"><a target="_blank" href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/attune-nxt-flow-cytometer.html" class="button navy">Manufacturer Site</a></p> </div> </div> </div></div></div> <div class="flow- views-row"><div class="views-field views-field-field-description"><div class="field-content"><div class="container"> <div class="flowimage"> <img src="/sites/default/files/2022-08/Flow%20Equip-659%20copy.jpg" /> </div> <div class="flowtext"> <h4>Amnis Imagestream X Mark II</h4> <p>The Amnis Imagestream X Mark II is an imaging cytometer that combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. The Imagestream comes equipped with 20, 40 and 60X magnification, as well as the ability to detect up to 6 fluorescent channels (or 4 plus brightfield and side scatter) using 4 lasers (405, 488, 561 and 635nm). Cells can be run at up to 5,000 events per second.</p> <p><strong>Applications for the Imagestream include:</strong></p> <ul> <li>Co-localization</li> <li>Internalization</li> <li>Cell cycle and mitosis</li> <li>Cell signaling and interaction</li> <li>Shape change and chemotaxis</li> <li>Nuclear translocation</li> </ul> <p>Practical and theoretical training is available, as well as experimental and data analysis support.</p> <p id="manu-butt"><a target="_blank" href="https://www.luminexcorp.com/imagestreamx-mk-ii/#overview" class="button navy">Manufacturer Site</a></p> </div> </div> </div></div></div> </div> <div class="view-footer"> <style> .flow-7270{padding-top:72px;padding-bottom: 40px} .flow- { padding-top: 72px;padding-bottom: 30px} .flow- #manu-butt{margin-top:25px !important} </style> </div> </div> </div> </div> <div id="block-donate" class="clearfix block block-block-content block-block-content088590df-5b08-415b-9ea8-aba4578a4a67"> <div class="content"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><div class="container"> <h2>Support Discovery Science</h2> <p>Your gift to Gladstone will allow our researchers to pursue high-quality science, focus on disease, and train the next generation of scientific thought leaders.</p> <p><a class="button magenta" href="https://gladstone.org/make-a-gift">Donate Now</a></p> </div> </div> </div> </div> </section> <section id="faqs"> <div class="accordion-wrapper"> <div class="container"> <h2>FAQs</h2> <div class="accordion accord1"><h3>How Do I Book a Session on a Flow Cytometer or Cell Sorter?</h3></div> <div class="panel"> <p>You’ll first need to <a href="https://gladstone.corefacilities.org/account/34/signup">register for an iLab account.</a> Once that’s complete, you should read, sign, and return the signature page of the<a href="https://padlet.com/janesrivastava5/Bookmarks/wish/2400933340"> general regulations document</a> to the core <a href="mailto:flow-core@gladstone.ucsf.edu">by email.</a></p> <p><a href="mailto:flow-core@gladstone.ucsf.edu">Contact core staff</a> if you have not used the core before.</p> </div> <div class="accordion"><h3>Which Flow Cytometers Are Capable of Running Plates?</h3></div> <div class="panel"> <p>The Fortessa X-20, Attune NxT, and Cytek Aurora, are capable of analyzing 96- and 384-well plates.</p> </div> <div class="accordion"><h3>How Do I Get Trained to Use the Flow Cytometry Core?</h3></div> <div class="panel"> <p>If you'd like to be trained on an analyzer or sorter, complete <a href="https://forms.gle/eWvAiBMTuY4AfCt17">Gladstone's Flow Core intro questionnaire</a> to answer some questions about your experience, and email the core to schedule a date.</p> </div> <div class="accordion"><h3>What Type of Samples Can Be Run on the Cytometer?</h3></div> <div class="panel"> <p>Any single-cell suspension where cells are between 500 nm and 20 μm can be run. Samples can be provided in 5-ml Falcon tubes, 15-ml Falcon tubes, Eppendorf tubes, multi-well plates, or slides.</p> <p>Cells can be at a concentration of up to 5 million per mL in PBS or media, but serum should be limited to 2 percent serum, as more than this can affect the charge applied to the stream. You can also add HEPES (25 mM), EDTA (1 mM) or DNAse (10 U/ml) to avoid clumping. If you are concerned that your sample is too concentrated, bring in some media to dilute your sample. Filter your cells through a cell strainer or filter-top tube.</p> </div> <div class="accordion"><h3>Can the Cytometers Be Used Independently, After Hours, or on Weekends?</h3></div> <div class="panel"> <p>After being trained, you must demonstrate proficiency in cytometer operation, maintenance, and basic troubleshooting before being allowed to use the equipment independently or outside of operating hours.</p> </div> <div class="accordion"><h3>Does the Flow Core Archive Data?</h3></div> <div class="panel"> <p>The core saves a copy of the FCS files to an internal server.</p> </div> </div> </div> <div class="corebk5"> </div> </section> </div> </div> </div> <script src="/libraries/easytabs/jquery-1.7.1.min.js" type="text/javascript"></script> <script src="/libraries/easytabs/jquery.hashchange.min.js" type="text/javascript"></script> <script src="/libraries/easytabs/jquery.easytabs.min.js" type="text/javascript"></script> <script type="text/javascript"> $(function( $ ){ $('#subtabs').easytabs(); }); </script> <script src="//ajax.googleapis.com/ajax/libs/jquery/1.11.0/jquery.min.js"></script> <script src="/libraries/owl-carousel2/owl.carousel.js"></script> <script> var owl = $('.owl-carousel'); owl.owlCarousel({ margin: 10, lazyLoad:true, lazyLoadEager: 1, loop: true, dots: false, responsive: { 0: { items: 1 }, 600: { items: 2 }, 1000: { items: 3, nav: true } } }) // disable scroll owl.on('drag.owl.carousel', function(event) { document.ontouchmove = function (e) { e.preventDefault() } }) // enable scroll owl.on('dragged.owl.carousel', function(event) { document.ontouchmove = function (e) { return true } }) </script> <script> if ($('#datalist li').length <= 15) { $('a.morepub ').hide(); } if ($('p.firstWord').length <= 15) { $('a.moreawards ').hide(); } $(function () { $('a.morepub').click(function () { $('#datalist li:hidden').slice(0, 15).show(); if ($('#datalist li').length == $('#datalist li:visible').length) { $('a.morepub ').hide(); } }); }); </script> <script> var acc = document.getElementsByClassName("accordion"); var i; for (i = 0; i < acc.length; i++) { acc[i].addEventListener("click", function() { this.classList.toggle("active"); var panel = this.nextElementSibling; if (panel.style.maxHeight){ panel.style.maxHeight = null; } else { panel.style.maxHeight = panel.scrollHeight + "px"; } }); }</script> <script> // This prevents the page from scrolling down to where it was previously. if ('scrollRestoration' in history) { history.scrollRestoration = 'manual'; } // This is needed if the user scrolls down during page load and you want to make sure the page is scrolled to the top once it's fully loaded. 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