An Investigation of the Use of Visible Spectrophotometric Analysis of Lead in an Herbal Tea Supplement

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Aquino</a>, <a href="https://publications.waset.org/abstracts/search?q=Ma.%20Veronica%20Aranda"> Ma. Veronica Aranda</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikki%20Francine%20Balde"> Nikki Francine Balde</a>, <a href="https://publications.waset.org/abstracts/search?q=Angeli%20Therese%20F.%20Cruz"> Angeli Therese F. Cruz</a>, <a href="https://publications.waset.org/abstracts/search?q=Elise%20Danielle%20Garcia"> Elise Danielle Garcia</a>, <a href="https://publications.waset.org/abstracts/search?q=Antonie%20Kyna%20Lim"> Antonie Kyna Lim</a>, <a href="https://publications.waset.org/abstracts/search?q=Divina%20Gracia%20Lucero"> Divina Gracia Lucero</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikolai%20Thadeus%20Mappatao"> Nikolai Thadeus Mappatao</a>, <a href="https://publications.waset.org/abstracts/search?q=Maylan%20N.%20Ocat"> Maylan N. Ocat</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamille%20Dyanne%20L.%20Pajarillo"> Jamille Dyanne L. Pajarillo</a>, <a href="https://publications.waset.org/abstracts/search?q=Jane%20Mierial%20A.%20Pesigan"> Jane Mierial A. Pesigan</a>, <a href="https://publications.waset.org/abstracts/search?q=Grace%20Kristin%20Viva"> Grace Kristin Viva</a>, <a href="https://publications.waset.org/abstracts/search?q=Jasmine%20Arielle%20C.%20Yap"> Jasmine Arielle C. Yap</a>, <a href="https://publications.waset.org/abstracts/search?q=Kathleen%20Michelle%20T.%20Yu"> Kathleen Michelle T. Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Joanna%20J.%20Orejola"> Joanna J. Orejola</a>, <a href="https://publications.waset.org/abstracts/search?q=Joanna%20V.%20Toralba"> Joanna V. Toralba </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lead is a neurotoxic metallic element that is slowly accumulated in bones and tissues especially if present in products taken in a regular basis such as herbal tea supplements. Although sensitive analytical instruments are already available, the USP limit test for lead is still widely used. However, because of its serious shortcomings, Lang Lang and his colleagues developed a spectrophotometric method for determination of lead in all types of samples. This method was the one adapted in this study. The actual procedure performed was divided into three parts: digestion, extraction and analysis. For digestion, HNO3 and CH3COOH were used. Afterwards, masking agents, 0.003% and 0.001% dithizone in CHCl3 were added and used for the extraction. For the analysis, standard addition method and colorimetry were performed. This was done in triplicates under two conditions. The 1st condition, using 25碌g/mL of standard, resulted to very low absorbances with an r2 of 0.551. This led to the use of a higher concentration, 1mg/mL, for condition 2. Precipitation of lead cyanide was observed and the absorbance readings were relatively higher but between 0.15-0.25, resulting to a very low r2 of 0.429. LOQ and LOD were not computed due to the limitations of the Milton-Roy Spectrophotometer. The method performed has a shorter digestion time, and used less but more accessible reagents. However, the optimum ratio of dithizone-lead complex must be observed in order to obtain reliable results while exploring other concentration of standards. <iframe src="https://publications.waset.org/abstracts/10463.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=herbal%20tea%20supplement" title="herbal tea supplement">herbal tea supplement</a>, <a href="https://publications.waset.org/abstracts/search?q=lead-dithizone%20complex" title=" lead-dithizone complex"> lead-dithizone complex</a>, <a href="https://publications.waset.org/abstracts/search?q=standard%20addition" title=" standard addition"> standard addition</a>, <a href="https://publications.waset.org/abstracts/search?q=visible%20spectroscopy" title=" visible spectroscopy"> visible spectroscopy</a> </p> <a href="https://publications.waset.org/abstracts/10463/an-investigation-of-the-use-of-visible-spectrophotometric-analysis-of-lead-in-an-herbal-tea-supplement" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10463.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">392</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> 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An Investigation of the Use of Visible Spectrophotometric Analysis of Lead in an Herbal Tea Supplement