CINXE.COM

<!DOCTYPE html><html lang="en-US"><head><title>Just a moment...</title><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><meta http-equiv="X-UA-Compatible" content="IE=Edge"><meta name="robots" content="noindex,nofollow"><meta name="viewport" content="width=device-width,initial-scale=1"><style>*{box-sizing:border-box;margin:0;padding:0}html{line-height:1.15;-webkit-text-size-adjust:100%;color:#313131;font-family:system-ui,-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Helvetica Neue,Arial,Noto Sans,sans-serif,Apple Color Emoji,Segoe UI Emoji,Segoe UI Symbol,Noto Color Emoji}body{display:flex;flex-direction:column;height:100vh;min-height:100vh}.main-content{margin:8rem auto;max-width:60rem;padding-left:1.5rem}@media (width <= 720px){.main-content{margin-top:4rem}}.h2{font-size:1.5rem;font-weight:500;line-height:2.25rem}@media (width <= 720px){.h2{font-size:1.25rem;line-height:1.5rem}}#challenge-error-text{background-image:url(data:image/svg+xml;base64,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);background-repeat:no-repeat;background-size:contain;padding-left:34px}@media (prefers-color-scheme:dark){body{background-color:#222;color:#d9d9d9}}</style><meta http-equiv="refresh" content="120"></head><body class="no-js"><div class="main-wrapper" role="main"><div class="main-content"><noscript><div class="h2"><span id="challenge-error-text">Enable JavaScript and cookies to continue</span></div></noscript></div></div><script>(function(){window._cf_chl_opt={cvId: '3',cZone: "publications.waset.org",cType: 'non-interactive',cRay: '9134f9166e494dce',cH: 'tzyby8qAPcKhZj45oqaQ4nSLo4KuJfuqRB8wuk4Oulw-1739786873-1.2.1.1-guze9GLEm27Jiu8Rrjyd7XeHU9S_839pai2eKRbVm6uI_rFdryaz7kAci6g.T7Ja',cUPMDTk: "\/abstracts\/search?q=primer%20generation&__cf_chl_tk=Rp_S6B5B4AriNIs4EvQIBSXidgPrk6BrI0UmcNYwir8-1739786873-1.0.1.1-ToXbupqB5t2rv2ktc4CoM2dpclQlicYq4inMxRf0YPM",cFPWv: 'b',cITimeS: '1739786873',cTTimeMs: '1000',cMTimeMs: '120000',cTplC: 0,cTplV: 5,cTplB: 'cf',cK: "",fa: "\/abstracts\/search?q=primer%20generation&__cf_chl_f_tk=Rp_S6B5B4AriNIs4EvQIBSXidgPrk6BrI0UmcNYwir8-1739786873-1.0.1.1-ToXbupqB5t2rv2ktc4CoM2dpclQlicYq4inMxRf0YPM",md: "fQd_lv3bFKVE4sm3cVfVR5zWukPlkNT.YhX8Avacios-1739786873-1.2.1.1-hfwGn51bgD67VxMMvhfz5n8t_ir305M_oHSoe55hV7FrenkSgnSA16UswX_43.kVA6__GZyZCsuPT76.gZ.FQ4Z1hkYNeZmj8vzfrUCNIkqtfGDftrIxrnc8GTxfCN5h3DVFn4eCue6FTHjaQrfVKuIqd.o9t7hhGbPZutBWV7NvQxApRJ3mIYuy0nT05X5KZ2oKRpYSXPRWykpzmrqAS6l5FSgA73EXEmCat_jvxpRwaAdIGHRhCcg_ZKT0LhrHHDGOpCBUefrfrwhnJlbGgjh9B6yYloJ9KQEH_93EtjRlyyxkCTIxHjI5E5GuNu_qwJQu0E1IRSwreu5hDqEVKKTUiFviRi27f9RO_xrtMl9Cb6juGSqh2IAg_2Kd4Qf8jo7WtKSh6pQcrjurTYFfqB5a4Z1SERGa2iZ5C6CVr1GaW1bxKTjd6zS3whTKkiOrMP1k1XKZqGUczYyWU53yFjgHS5mOhS3wFKV00252VTsukXtQboNfLusLYvOk5FV_WIjeDTNXU7tYFuCjv3w5H_eLCvtlGCxU4rdcM3Tbd1vRCNFIvX5XHafHviiG6hSEmORkkiUXwgZFzyOfbwaiV5_Rglz75xsfUrTAaIisoblQYawaOP8pgqoeZ_s3mpm24zZp6_AQqcJeyO9kc4NAeg2wq8.nSzKNJBkY3GpL4D6qzNm25pdPoxvUtFP5P7HzH2e3HxCXwce9vqmwZEuAhEnQF9I5qcSpwL3PRJmrcQO.BTNIy2pc6pN3_AAlmcEfdltxkN8JTDrwGppD4s5bcHWRRDQrXyjlW_k3bvfirRYGYyzmhsrnJ0W7HCsyPcDYPUb5TW.5U42aDu42aIBh5A8r671Z6bVvqwtpreFifNzhdopXTjF4eBwOGAeaopsSb9X4E.R1AH6OkHwEZBLRW.NeI.zk11PM3N6vZb3JFlc7PaOokharOD_AvucQj.vBKk2mYeG6xKJ20acqOrylZ8Xcu3.aHzCwk3N4yf8cs7NqoGwz3r8xY0u90bEa1HDG4w.xAlRYcWwBhF.P2i7JBhusjwdWF8tCn3YX5z0PhGmlxjDxYp2BAkhf9qLM288TOlobXr_5mhDR8mciCakSmDEbXz0dSPlz8OkZH26Z7ZvEMYnYFbujTiTVXc5gZ6s2IY4mEkKbFf28DgNjwEjuPccgyJMgjqw4OzTFVyhrdk61XeF6KpL9Eo2m6GWlOYzMea3emjuE7zN99cwPBCMD6CKpVlXySuJjFV8PXT7sAkySs2XvD_Xv7sPWLyc6OP7NvS2fnRGEVrSgJqFs00ghsPWiFtuSqJLOsVqUJwRD0h8mgSgygWyCWUzUWcvSk.jZJpxIfmHyNQWDN8T2Y7ojPfJHcdBfe9afdCxsQErS5cJbcov3nvWZGcWKO4plYJpaSIEqptZp_h2_8hpNzATy4j69WbbUinTQHq8oXiEyDygSQNG4U5JEYbOBN6ugwsyBXQmezJl2XJUdaZjE3tqYJ.T12RrRjGz3Rsf08bCD023yduPvzN41yDHMONr_a2bTYg4gVNmDrG4rtWjpqYydPxYQJ.fGxVxoAPPdPSpl_TcbludDqCS0cp3W0X8q8VzqVGx9yb9kh4sHHFl2BTigGUIz6qJJwSlCa8_ceAXDVt.mg1mzBtDBr.f7gDuMfxsk1b7Gs7KehViPcYxS4IuRKBNvOkwFHYX3F4c7NGBCSnSxeAWuk3QeTc2f3JbRy0NDcDYUncCKT9nLyvE5e8KTw8JoYk9OIybbjZyfI6h3d.ATM4snDCnCfFVZ5l7prG66.BDam9869fngogozExah8oLE1xBy5_145RS9IJBbzegKiDuFL3.A7vDI.lPdtHt6D20UnoBxTqzh9KGcstYkpxIYQj_GiS6jgZpAVEjTWFiddJsPhKdfdhBlGX5O65cihN96WzSkp.UUOZmQt_Q4s9VOUCQxw63eHdYrQw3FXcU",mdrd: "UMkjzYlAm.qb3kGuvIp9ESrryqQqOl.csnSkHLlZDe0-1739786873-1.2.1.1-QodZhxHsDxsjglNjbllppjLgxQYsd29EPVJBf1e0O1P5re.jlll57e3twb7UntBJCw5Bx3NWTxnLbD8W_ZVkaYcsyYWlulDuRmOSL_UyJ7xO._5_x3ZhT_Z8ovl0zCTLRBLWfL9PrOAzDCEp4cXRLkk1NTYSVhCK9kIwINXy8tjGS4XEwsHtK6sAtKWqjKqijI9tQa3N0syadrmG879MLt2LyuNH6uoMvFXlSiAiXvsT3Q7ayShBWFPYV0KMeE7MQhTIP79YOayNrJx5Tf9lUjwVUHDe99G5k8gX8nWI6hkzbPl11JyQ.Xh_64XTFCKK0G8qPPbVcp_HKzT.qmgOzL.GGbSFgOID.Y2Q8niIwUzxi9TGiackeQqW0R45DBMPEvwFDVp4XgmvRNl5zGL72fqpcGVPw_LXZjGON3YjomRnPGB8FTyo.49ge3OO.kJa_ndZoqmY7QGQ7dv5EVvzZe557JQzxnC1854YEbBOwnl2rX3pQISIAx.PbgxUD9popoWbwRlv7kr26F7.HszTxlLqBTpr1Mpqw4s.Bk5sGUHlgXlMHfCJcgpCAEx_sfL49c1BAlzBjLFwEoftvCM9tRaP58Hm6p8upefWCM3SBzYuFe3mrh9ishzdnf4KXuXahxf_c9PgdiVqMBK6P9lGulcjczGYj_mTpK5IKCh_KJenXDIQlcsyaZINYtIYBj6IaGtf_FiXUpeGwDpJ8jvkKJR0MXuy2GhBoXhQ97GDDf7KmnGjRt7lQ3G_tU1bPUyUGuJY1fE7Glx2vcp2bzZ00MIF42RvjKYf08G1yAvZzrE1bGJrfz8eS1syeRZyGEIgdRNhbTPB.L0TNZNtv7I1XzSCS_3ScmqncYn.u43v9CwvMp5OyoFycq7eVlLirVTnEVerZknVfHn0mTwKWlRyteMpmOM5IF5QjYZQCdXTlD2pvz.XUgbPn.VDKoGJgUsyRkuiSnVwghKiO5nIr4MfxrJWwMxyfQIxT.O08CwAYBKhTTfyllen5Jz1Obne3YuK2Tvkf3BUdQ6vxjkFb2EN1NLBmDxK.0mtH36xT1vZJiLDWziWk.WmNOs6zp0tiauf9xUxTe5rKF1IWU43aefJFCDvuDqM1MM_jWHea8STCTEu0aIHhjp1zSvr5IvxUr3xFVh563QF4TJ5l.363.qJBP.DHN.UWN6JWFJqXrOgRDK8syMiP0pzY9j8nOXOd8rfUK68giqusI9SPr7t786BJFOU0oq.oB.cdi9zuAKl5D3.yvPgVZuY1QnBI9QsncN77dMQ0.YNx5sbx_zGQA2VVjglORvOtCM7yczBT5jL7ZstKEn4GUai8VjMkfzAMNDLN9yQOe6aCzJSXc1nNess22xpRBDSbvM7Qtvk29Qj7WVPLY9kJVWLsFjeG5MQdDSyQDww3tEGckbVVLPwkDueYNFvoCB8uYzBUF3Cifm4neaH7VuAB5vfqp4KnpVDmePAFQe8UEROowWnsPsrG9eppvxFu95k9pw05L48K0qRdDObqoed9xI_Q4qUPd2oJ_AItGCy7UihgLQzS5GygAX9XvwtNBeHkfzhPs6gTc.7_Xwf8725wYnXQ3quFRmq2.mfqKDki6vO7zMJZ08IaFt8vxHOiGLoqLjLNRXKpHU0tpdNtavn5txcVZLMYAlymsW002lOwJHWEeWKpThPxYVuuihaeIDDSKPI_fB.xC1JZW6JM2zoisZuP8nrpNzgdUDEjS0v_Le_ImAyI9s6IPJA9r0J.IM1lgClfkIkQa_WluT6ta1OQoQLGoIymJ4J1UpfLO0vsmVOTXPHyd27775kprcPT1F_SVNjBiNczjNckwwcIoKWyzn6UF4uJGphrACq3RA8utZLVJcsVsegeSx4Yck1ChkSEd3Ur4INU9NufmFOFPkqyFtdQ7r69iRcjSmwxT2RJSsUkqcGEyaI3D3RqJCXp_lpfaEQEA86ZdlEw7IybMSumOtpmAozHoRIi9Uf"};var cpo = document.createElement('script');cpo.src = '/cdn-cgi/challenge-platform/h/b/orchestrate/chl_page/v1?ray=9134f9166e494dce';window._cf_chl_opt.cOgUHash = location.hash === '' && location.href.indexOf('#') !== -1 ? '#' : location.hash;window._cf_chl_opt.cOgUQuery = location.search === '' && location.href.slice(0, location.href.length - window._cf_chl_opt.cOgUHash.length).indexOf('?') !== -1 ? '?' : location.search;if (window.history && window.history.replaceState) {var ogU = location.pathname + window._cf_chl_opt.cOgUQuery + window._cf_chl_opt.cOgUHash;history.replaceState(null, null, "\/abstracts\/search?q=primer%20generation&__cf_chl_rt_tk=Rp_S6B5B4AriNIs4EvQIBSXidgPrk6BrI0UmcNYwir8-1739786873-1.0.1.1-ToXbupqB5t2rv2ktc4CoM2dpclQlicYq4inMxRf0YPM" + window._cf_chl_opt.cOgUHash);cpo.onload = function() {history.replaceState(null, null, ogU);}}document.getElementsByTagName('head')[0].appendChild(cpo);}());</script></body></html>