Thin Film Spectroscopy: Setup and Measurement

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The Ossila Optical Spectroscopy Kit supplies everything you need to start taking measurements on thin films." /><link rel="apple-touch-icon" sizes="180x180" href="//www.ossila.com/cdn/shop/t/303/assets/apple-touch-icon.png?v=47689890648139992551729065668"> <link rel="icon" type="image/png" sizes="32x32" href="//www.ossila.com/cdn/shop/t/303/assets/favicon-32x32.png?v=170914528706734454101729065668"> <link rel="icon" type="image/png" sizes="16x16" href="//www.ossila.com/cdn/shop/t/303/assets/favicon-16x16.png?v=162550173345868048431729065668"> <link rel="manifest" href="//www.ossila.com/cdn/shop/t/303/assets/site.webmanifest?v=100969836723433583841729065669"> <link rel="mask-icon" href="//www.ossila.com/cdn/shop/t/303/assets/safari-pinned-tab.svg?v=95647162979764929801729065669" color="#39499c"> <meta name="msapplication-TileColor" content="#418fde"> <meta name="theme-color" content="#ffffff"> <link rel="canonical" href="https://www.ossila.com/pages/thin-film-spectroscopy-setup-measurement" />  <link rel="stylesheet" href="//www.ossila.com/cdn/shop/t/303/assets/main-style.css?v=129786468726129743101731684242">  <script>var shopifyMoneyFormat = '${{amount}}';</script> <script defer src="//www.ossila.com/cdn/shop/t/303/assets/main.js?v=120109391065215852831729088283"></script>  <meta property="og:type" content="website" /> <meta property="og:title" content="Thin Film Spectroscopy: Setup and Measurement" /> <meta property="og:description" content="Thin Film Spectroscopy Setup and Measurement article to get you started on taking optical spectroscopy measurements of thin films. The Ossila Optical Spectroscopy Kit supplies everything you need to start taking measurements on thin films." /> <meta property="og:url" content="https://www.ossila.com/pages/thin-film-spectroscopy-setup-measurement" /> <meta property="og:site_name" content="Ossila" /> <meta name="twitter:site" content="@ossilascience"> <meta name="twitter:card" content="summary"> <meta name="twitter:title" content="Thin Film Spectroscopy: Setup and Measurement"> <meta name="twitter:description" content="You can use your USB spectrometer to measure the absorbance and transmission of visible light through thin films. You can also measure other properties of thin films using this technique, such as photoluminescence. The Ossila Optical Spectroscopy Kit comes with everything you need in order to conduct these measurements on thin films. This article contains some advice from our researchers that should help you get started taking optical spectroscopy measurements of thin films. Fabricating your Samples For optical spectroscopy experiments, we recommend depositing your sample on a quartz substrate. Quartz-coated glass is ultra-smooth, and it is not absorbent in the visible light region, so it shouldn't interfere with the optical or morphological properties of your device. It is important that you try to reduce the number of defects and pinholes in your film where you can. 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You can still place orders by emailing us on <a href="mailto:info@ossila.com">info@ossila.com</a>, but you may experience issues browsing our website. Please consider upgrading to a modern browser for better security and an improved browsing experience.</p> </div> </div>  <main> <div id="informational-page"> <h1>Thin Film Spectroscopy: Setup and Measurement</h1> <div class="container"> <img width="1340" src="https://www.ossila.com/cdn/shop/files/broadband-white-light-source-breadboard.jpg?crop=center&width=1340&height=280" loading="lazy" height="280" class="featured-image" alt="Thin Film Spectroscopy: Setup and Measurement"> <p>You can use your <a href="/products/optical-spectrometer">USB spectrometer</a> to measure the <a title="Absorbance Spectroscopy" href="https://www.ossila.com/pages/absorbance-spectroscopy">absorbance</a> and transmission of visible light through thin films. You can also measure other properties of thin films using this technique, such as <a title="Photoluminence Spectroscopy" href="https://www.ossila.com/pages/photoluminescence">photoluminescence</a>. The Ossila <a href="/products/optical-spectroscopy-bundle">Optical Spectroscopy Kit</a> comes with everything you need in order to conduct these measurements on thin films. This article contains some advice from our researchers that should help you get started taking optical spectroscopy measurements of thin films.</p> <h2>Fabricating your Samples</h2> <hr> <p>For <a title="Optical Spectroscopy Article" href="https://www.ossila.com/pages/optical-spectroscopy">optical spectroscopy</a> experiments, we recommend depositing your sample on a quartz substrate. <a title="Ultra-Flat Quartz Coated Glass Substrates" href="https://www.ossila.com/products/ultra-flat-quartz-coated-glass">Quartz-coated glass</a> is ultra-smooth, and it is not absorbent in the visible light region, so it shouldn't interfere with the optical or morphological properties of your device.</p> <p><span>It is important that you try to reduce the number of defects and pinholes in your film where you can. This is because defects can have different optical properties from the bulk material, and this will affect your results. </span><span>If you are depositing your film from solutions, you may wish to heat, agitate, or filter your solution before coating your substrate. This will break up or remove any aggregates or impurities. </span></p> <p><span>Additionally, it is also important to ensure that your substrates are clean before you coat them. To assure that your substrates are clean, we recommend that you rinse and sonicate them in a <a title="Substrate Racks" href="https://www.ossila.com/products/substrate-rack">substrate rack</a> in several solutions (<a title="Hellmanex III for Critical Cleaning" href="https://www.ossila.com/products/hellmanex-iii">Hellmanex</a>, DI water, IPA, acetone), using a pressurised gas flow to dry them between each solution. Next, expose your substrates to a <a title="Ossila UV Ozone Cleaner" href="https://www.ossila.com/products/uv-ozone-cleaner">UV Ozone Cleaner</a> for several minutes before coating to increase their wettability.</span></p> <p>Good news though - your sample doesn't have to be completely perfect for you to take an optical spectroscopy measurement. Actually, only a small area of your sample will be in the beam path, so try to make sure that you position a "nice" uniform area of your sample here. The Ossila <a href="/products/optical-spectrometer">USB Spectrometer</a> will focus the light into a small area of 1.6 mm - 1.8 mm diameter. The approximate placement of this beam on a 15 mm x 20 mm substrate (placed in vertically) is shown in the diagram below.</p> <p> </p> <figure><img width="424" src="https://www.ossila.com/cdn/shop/files/Spectrometer-substrate-placement.svg?v=1675180978" loading="lazy" height="415" alt="15 mm x 20 mm Substrate Area of Illumination"> <figcaption>Area that will be illuminated (grey). Anywhere along horizontal band can be measured by moving the substrate laterally.</figcaption> </figure> <p>If you are taking absorbance measurements from particularly thick or dark films, they may not transmit enough light through for you to get an accurate absorbance measurement. Instead, your measurement may have a low peak intensity and a lot of noise. In this case, you may need to dilute your precursor solution more before creating a thin film.</p> <a href="https://www.ossila.com/products/optical-spectrometer" data-track-content="" class="green-banner"> <p data-content-piece="cta-optical-spectrometer" data-content-name="/pages/thin-film-spectroscopy-setup-measurement">Optical Spectrometer</p> <img width="600" src="https://www.ossila.com/cdn/shop/files/Ossila-Optical-Spectrometer-Kit-Fluorescence.jpg?crop=center&width=600&height=120" loading="lazy" height="120" alt="Optical Spectrometer"> <button>Browse now</button> </a> <h2>Taking a Measurement</h2> <hr> <p><span>The Ossila spectrometer comes with its own <a title="Optical Spectrometer Software" href="https://www.ossila.com/pages/software-drivers#Optical-Spectrometer">software</a> that makes taking a spectroscopy measurement as easy as possible. </span></p> <p><span>Before you begin, you need to decide if you want to use optical fibre cables or measure your sample directly through air. If you measure the light directly, make sure that all of your equipment is aligned using the optical breadboard, and do not move the components relative to one another once you take your first measurement. </span></p> <p>There are different optimum set-ups for each of these relative various components. The ideal set-up for you is going to depend on both your sample and on which measurement you want to take. For strongly fluorescent samples, we recommend a 45° angle between the incident light and the output measurement. You can set this up using our <a title="Spectroscopy Transmission Holder with 4 Ports" href="https://www.ossila.com/products/transmission-holder?variant=43371016028376">4-port transmission sample holder</a>.</p> <figure><img width="424" src="https://www.ossila.com/cdn/shop/files/fluorescence-measurement-45-degree-angle.jpg?v=1675077002&width=424&height=283" loading="lazy" height="283" alt="Ideal measurement set up for photoluminescence is 45 degrees"> <figcaption>The ideal measurement setup for photoluminescence is 45 degrees.</figcaption> </figure> <figure> <p><span style="font-weight: 400;">If you are measuring absorbance and transmission, your input light source and your output measurement beam must be parallel. This is the only way that you will be able to measure the direct transmission through your sample. </span></p> <img width="424" src="https://www.ossila.com/cdn/shop/files/fluorescence-measurement-180-degree-angle.jpg?v=1675077003&width=424&height=283" loading="lazy" height="283" alt="Ideal measurement set up for absorbance measurements"> <figcaption>Ideal measurement setup for absorbance measurements.</figcaption> </figure> <p>Additionally, for absorbance and transmission measurements, you must take a reference spectrum through a blank quartz film. <strong> If you change anything during your experiment, you must retake this reference measurement.</strong> This is because, for both transmission and <a title="Absorbance Spectroscopy" href="https://www.ossila.com/pages/absorbance-spectroscopy">absorbance spectroscopy</a>, you are comparing the light travelling through your sample to this reference measurement. If any variables (such as integration time) change between measurements, the absorbance units can become un-physical.</p> <h2>Measurement Variables</h2> <hr> <p>Within the Ossila Optical Spectrometer software, there are some variables which you can change to optimise your measurements.</p> <h3 id="integration-time"><strong>Integration Time</strong></h3> <p><span style="font-weight: 400;">Integration time dictates how long your spectrometer will collect photons before it begins to process this data. With a smaller integration time, you will receive a short snapshot of the optical properties of the material. This is useful if your material has a very strong optical signal, as it allows you to measure the optical properties of your sample without flooding the intensity threshold of the spectrometer.</span></p> <p>In most cases, by increasing the integration time, you will increase the number of photons collected from your sample. This will improve your signal-to-noise ratio and increase the intensity of your peaks.</p> <figure><img width="424" src="https://www.ossila.com/cdn/shop/files/Ossila-spectrometer-varying-integration-time.png?v=1674230694&width=424&height=279" loading="lazy" height="279.66" alt="fluoresence intensity variation with integration time"> <figcaption>Fluorescence intensity from a PFO film. Integration time of measurement is varied from 5 ms to 1000 ms.</figcaption> </figure> <p><span style="font-weight: 400;">The above figure shows how the <a href="/pages/fluorescence-spectroscopy">fluorescence</a> intensity of PFO increases as the integration time increases. It also shows how, if this integration time is too long, the measured signal will exceed the limits of the spectrometer. Here, </span><i><span style="font-weight: 400;">Integration Time = 1000 ms</span></i><span style="font-weight: 400;"> and the fluorescence peaks at 430 nm - 450 nm are cut off. If this occurs with your measurements, you will get a warning saying, “Spectral intensity maximum exceeded".</span></p> <p>If you change the integration time when you take absorbance measurements, you must retake the background and <strong>reference spectra</strong>. This is because the absorbance units will be proportional to the light intensity measured through your sample at each wavelength, divided by the intensity measured through your reference sample at that wavelength.</p> <figure><img width="129.55" src="https://www.ossila.com/cdn/shop/files/equation-absorbance.svg?v=1674812091" loading="lazy" height="47.8" alt="Absorbance Equation - Light Intensity"></figure> <p>So, if you only increase the integration time for your current measurement, the spectrometer will collect a larger amount of light than it did for the reference measurement. You then won't be able to compare the reference measurement to your sample measurement as you will have changed the quantity of your measured light.</p> <p><span>Another effect of having different integration times for your reference measurement and your sample measurement is that </span><span>the absorbance spectra may fall below zero. At a specific wavelength, if I ≥ I</span><span>0</span><span>, then absorbance falls below zero. This doesn't make physical sense, as it suggests that the sample is producing light, which most absorbent materials do not do under </span><a href="https://www.ossila.com/products/broadband-white-light-source"><span>broadband light</span></a><span>. </span><span><span style="font-weight: 400;">At the wavelengths where light is transmitted through your sample, the spectrometer will collect more light for the experimental measurement than it collected for the reference sample. This is due to the different integration times and will result in inaccurate absorbance values on the y-axis.</span> You can still use peak shape and placement using this data, but it will need to be normalised between 0 and 1. Using this data, you cannot extract an absorption coefficient. </span></p> <h3><strong>Delay</strong></h3> <p>The delay setting allows you to set up a delay between successive measurements. This might allow samples to "recover" between measurements and it can be useful if you are using materials that are sensitive to illumination. Using the delay setting will also ensure that luminescence from your previous measurements isn't included in your subsequent measurements.</p> <h3><strong>Accumulation</strong></h3> <p><span>If you change the accumulation number on your spectrometer, you will get a sum of multiple different spectra. For example, if you set it to five when you take a measurement, the Ossila software will take five individual successive spectra and add these up. This will give you a spectrum with five times the signal intensity of your initial measurement and will help reduce noise. This can be especially useful if you have low amounts of fluorescence or signal.</span></p> <h3><strong>Averaging</strong></h3> <p><span>If you change the averaging number on your spectrometer, you will get an average of multiple different spectra. If you set it to five when you take a measurement, the Ossila software will output an average of five different individual spectra. This means that your spectrum will be smoother. </span></p> <div class="inline-block-container"> <figure class="double-float-left"><img width="417.16" src="https://www.ossila.com/cdn/shop/files/PFO-averaging-number-1.png?v=1675069169&width=417&height=291" loading="lazy" height="291.09" class="fill" alt="PFO fluorescence - averaging number"> <figcaption>Fluorescence measurements of PFO film, varying the number of spectra averaged.</figcaption> </figure> <figure class="double-float-right"><img width="417.16" src="https://www.ossila.com/cdn/shop/files/P3HT-o-IDTBR-averaging-number.png?v=1675068200&width=417&height=284" loading="lazy" height="284.88" class="fill" alt="P3HT:o-IDTBR absorbance - averaging number"> <figcaption>Absorbance measurements of P3HT:o-IDTBR films, varying the number of spectra averaged.</figcaption> </figure> </div> <p>For both fluorescence and absorbance measurements, increasing the number of individual spectra that you average should increase the smoothness of your graph. For strong signals, such as PFO fluorescence, you only need to average over a few spectra as the signal is already quite strong. However, for measurements where there are low levels of light detection, such as absorbance measurements of <a title="P3HT Polymer" href="https://www.ossila.com/products/p3ht">P3HT</a>:<a title="o-IDTBR N-type Non-Fullerene Acceptor" href="https://www.ossila.com/products/o-idtbr">o-IDTBR</a> films, averaging over many spectra can help to significantly increase the signal-to-noise ratio.</p> <div class="panel panel-default"> <div class="panel-body"> <h3>Contributing Authors</h3> <hr> <p>Written by</p> <p class="no-margin"><a title="Author Biography: Mary O'Kane" href="/pages/our-writers#mary-okane">Dr. Mary O'Kane</a></p> <p class="text-muted">Application Scientist</p> </div> </div> <script type="application/ld+json"> { "@context": "https://schema.org", "@type": "Article", "abstract": "You can use your optical spectrometer to measure the absorbance and transmission of visible light through thin films. You can also measure other properties of thin films using this technique, such as photoluminescence. The Ossila Optical Spectroscopy Kit comes with everything you need in order to conduct these measurements on thin films. This article contains some advice from our researchers that should help you get started taking optical spectroscopy measurements of thin films.", "author": [ { "@type": "Person", "name": "Mary O'Kane", "honorificPrefix": "Dr.", "jobTitle": "Application Scientist", "url": "https://www.ossila.com/pages/our-writers#mary-okane" } ], "headline": "Thin Film Spectroscopy: Setup and Measurement", "image": ["https://www.ossila.com/cdn/shop/files/fluorescence-measurement-45-degree-angle.jpg" ], "inLanguage": "en", "publisher": { "@type": "Organization", "name": "Ossila", "url": "https://www.ossila.com" }, "url": "https://www.ossila.com/pages/thin-film-spectroscopy-setup-measurement" } </script> </div> </div> </main>  <div class="full-width-footer"> <footer id="footer" class="footer container"> <div class="clearfix footer-elems nav-footer" id="footer-links"> <div class="row"> <div class="col-xs-12 col-sm-3"> <span class="h2">Ossila</span> <ul class="nav-footer"> <li><a href="/pages/about">About Ossila</a></li> <li><a href="/pages/press-and-media">Press and Media</a></li> <li><a href="/pages/our-writers">Our Writing Team</a></li> <li><a href="/pages/awards-and-testimonials">Awards and Testimonials</a></li> <li><a href="/pages/feedback">Leave Feedback</a></li> <li><a href="/pages/terms-and-conditions">Terms and Conditions</a></li> <li><a href="/pages/privacy-policy">Privacy Policy</a></li> </ul> <span>Copyright © 2024 Ossila</span> </div> <div class="col-xs-12 col-sm-3"> <span class="h2">Join the Team</span> <ul class="nav-footer"> <li><a href="/pages/careers">Careers at Ossila</a></li> <li><a href="/pages/jobs-in-sheffield">Jobs in Sheffield</a></li> <li><a href="/pages/jobs-in-leiden">Jobs in Leiden</a></li> </ul> <span class="h2">Collaborate</span> <ul class="nav-footer"> <li><a href="/pages/reprinting-permissions">Free Digital Assets</a></li> <li><a href="/pages/conference-fund">Conference Sponsorship for Students</a></li> <li><a href="/pages/outreach-support">Science Outreach Support</a></li> <li><a href="/pages/graduation-awards-sponsorship">Graduation Awards</a></li> <li><a href="/pages/letters-of-support">Letters of Support</a></li> </ul> </div> <div class="col-xs-12 col-sm-3"> <span class="h2">Your Account</span> <ul class="nav-footer"> <li><a href="/account/login">Log In</a></li> <li><a href="/account/register">Create Account</a></li> </ul> <span class="h2">Order Information</span> <ul class="nav-footer"> <li><a href="/pages/how-to-order">How to Order</a></li> <li><a href="/pages/worldwide-shipping">Worldwide Shipping Charges</a></li> <li><a href="/pages/returns-and-cancellations">Returns and Cancellations</a></li> <li><a href="/pages/warranty-information">Warranty Information</a></li> <li><a href="/pages/distributors">Regional Distributors</a></li> <li><a href="/collections/deals-and-discounts">Deals and Discounts</a></li> <li><a href="/pages/price-drop-guarantee">Price Drop Guarantee</a></li> </ul> </div> <div class="col-xs-12 col-sm-3"> <span class="h2">Customer Support</span> <ul class="nav-footer"> <li><svg class="icon icon-envelope"><use xlink:href="#icon-envelope"></use></svg> <a href="/pages/contact-us">Send an Enquiry</a></li> <li><svg class="icon icon-envelope"><use xlink:href="#icon-envelope"></use></svg> <a href="mailto:info@ossila.com">info@ossila.com</a></li> <li class="no-padding-bottom"><a href="https://www.ossila.com/pages/about#our-locations" class="text-bold">Main Office</a></li> <li class="no-padding"><svg class="icon icon-phone"><use xlink:href="#icon-phone"></use></svg> +44 (0)114 2999 180</li> <li class="no-padding-top">Mon-Fri, 8:00-17:00 (GMT/BST)</li> <li class="no-padding-bottom"><a href="https://www.ossila.com/pages/about#our-locations" class="text-bold">EU Office</a></li> <li class="no-padding"><svg class="icon icon-phone"><use xlink:href="#icon-phone"></use></svg> +31 (0)718 081020</li> <li class="no-padding-top">Mon-Fri, 9:00-17:00 (CET/CEST)</li> </ul> </div> </div> <div class="text-center"> <span class="double-right-padding">Ossila Ltd</span> <span class="double-right-padding">Company Number 06920105</span> <span class="double-right-padding">VAT Number GB 978 2092 81</span> <span>EORI Number GB978209281000</span> </div> <div class="text-center"> <span class="double-right-padding">Ossila BV</span> <span class="double-right-padding">CCI number 84102241</span> <span class="double-right-padding">VAT Number NL 863097182B01</span> <span>EORI Number NL863097182</span> </div> </div> </footer> </div> <div id="bottom-banner" class="hidden"> <div id="cookie-banner" class="hidden"> <div class="container"> <div class="row"> <span class="col-xs-12 col-sm-8 no-margin pad-half-height">The Ossila website uses cookies for core functionality. 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