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'' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_85f6 .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_85f6 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="heading section"><h2> Generate highly specific targeted results </h2></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p>With its greater than 99% accuracy and long-read capabilities, Sanger sequencing is the gold-standard sequencing technology. And to further enable your research, fragment analysis by capillary electrophoresis (CE) provides DNA sizing, relative quantitation, and genotyping information. With our simple workflows, Sanger sequencing and fragment analysis can be completed in less than one workday—from sample to answer—helping to make your research of SARS-CoV-2 (the coronavirus that causes COVID-19) fast and cost-effective.</p><p><a class="btn btn-featured" href="/sg/en/home/global/forms/life-science/seqstudio-information-request.html" style="margin-bottom: 10px;">Speak to sales</a></p></div></div><div container-id="cq-colctrl-lt8" comp-id="B3"><div class="regionalwrapper parbase section"><div class="regionalparsys parsys"><div class="text parbase section"><p><a href="/sg/en/home/products-and-services/healthcare.html?icid=sg-vm-mer-leads-VMHealthcareR1-l5-bn-SarsCov2Research-072221" class="btn btn-featured">Speak to a Specialist</a></p></div></div></div></div><div container-id="cq-colctrl-lt8" comp-id="B4"><div class="regionalwrapper parbase section"></div></div><div container-id="cq-colctrl-lt8" comp-id="B5"><div class="regionalwrapper parbase section"></div></div></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3><span style="padding-top: 2px; padding-bottom: 2px;" class="label label-info">NEW</span> Sequencing protocols for SARS-CoV-2 variant detection </h3></div><div class="text parbase section"><p>Protocol for finding and using Sanger sequencing primers for any location in the SARS-CoV-2 genome, including new Variants of Concern <br><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Application-Notes/sars-cov-2-anywhere-protocol-app-note.pdf" target="_blank">Download protocol</a> | <a href="https://jbrowse-nc045512-2.thermofisher.com/" target="_blank">Visualize SARS-CoV-2 genome and Order Primers</a> | <a href="https://sars-cov2-sanger-primer-lookup.thermofisher.com" target="_blank">Search SARS-CoV-2 genome and Order Primers</a><br><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Application-Notes/sars-cov-2-variant-analysis-vr-protocol-app-note.pdf" target="_blank">Variant Analysis using Variant Reporter</a> | <a href="//downloads.thermofisher.com/20211201%20FinalVRFiles.zip" target="_blank">Files for Variant Reporter (zip)</a><br><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Application-Notes/sars-Cov-2-variant-analysis-ss-protocol-app-note.pdf" target="_blank">Variant Analysis using SeqScape</a> | <a href="//downloads.thermofisher.com/20211201%20FinalSSFiles.zip" target="_blank">Files for SeqScape (zip)</a></p><p>Protocol to quickly confirm S-gene dropouts seen with the TaqPath COVID-19 assay by identifying if the S gene 69/70 deletion is present <br><a href="https://assets.thermofisher.com/TFS-Assets/GSD/brochures/sequencing-sars-cov-2-s-gene-69-70del-protocol.pdf">Download protocol</a> | <a href="/us/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research.html#Ordering">Order primers</a></p><p>Flexible protocol to confirm the presence of the highly transmissible SARS-CoV-2 B.1.1.7, B.1.351, B.1.1.28, B.1.427 or B.1.429 strain lineages in your sample.  Pick and choose primers pairs that best suit your needs.<br><a href="http://assets.thermofisher.com/TFS-Assets/GSD/brochures/protocol_Sanger_sequencing_SARS-CoV-2_B117_B1351_brochure.pdf">Download protocol</a> | <a href="http://assets.thermofisher.com/TFS-Assets/LSG/Reference-Materials/SARS-CoV-2-britSA-variant-oligos.xls" target="_blank">Download primers sequences for B.1.1.7</a> | <a href="http://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/SARS-CoV-2_SA_variant_oligos_list.xls" target="_blank">Download primers sequences for B.1.351</a> | <a href="http://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/SARS-CoV-2_Brazil_variant_oligos_list.xls" target="_blank">Download primers sequences for B.1.1.28</a> | <a href="http://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/SARS-CoV-2-B.1.427-variant-oligos-list.xls" target="_blank">Download primers sequences for B.1.427</a> | <a href="http://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/SARS-CoV-2-B.1.429-variant-oligos-list.xls" target="_blank">Download primers sequences for B.1.429</a></p><p>Protocol to identify new variants in the Spike gene by sequencing the entire S-gene for vaccine development and epidemiological research.<br><a href="https://assets.thermofisher.com/TFS-Assets/GSD/brochures/sequencing-sars-cov-2-spike-gene-protocol.pdf">Download protocol</a> | <a href="https://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/Sanger_sequencing_oligos_SARS-CoV-2_gene_list.xls" target="_blank">Download primer sequences</a></p><p><a href="/blog/behindthebench/solutions-for-surveillance-of-the-s-gene-mutation-in-the-b117-501yv1-sars-cov-2-strain-lineage/">Learn more about which protocol to choose ›</a></p><p><a class="btn btn-primary" href="/order/custom-standard-oligo">Order Primers</a></p></div></div></div></div></div></div><div class="LTRawHTML section"><style> /*Badges*/ .npi { background-color: #333333; 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max-width: 350px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><div class="npi"><h3><a href="/blog/behindthebench/how-s-gene-target-failure-and-s-gene-sequencing-is-key-for-omicron-variant-surveillance/"> How S-gene Target Failure and S-gene Sequencing is Key for Omic...</a></h3><p><a href="/blog/behindthebench/how-s-gene-target-failure-and-s-gene-sequencing-is-key-for-omicron-variant-surveillance/">The COVID-19 global pandemic continues to disrupt people's... by Behind The Bench Staff / 12.10.2021</a></p><h3><a href="/blog/behindthebench/how-s-gene-target-failure-and-s-gene-sequencing-is-key-for-omicron-variant-surveillance/">Read More ›</a></h3></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_659f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.full.high.jpg/1660060916142.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.full.high.jpg/1660060916142.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.320.high.jpg/1660060916142.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.480.high.jpg/1660060916142.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.320.medium.jpg/1660060916142.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.620.high.jpg/1660060916142.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.320.low.jpg/1660060916142.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_659f.img.480.medium.jpg/1660060916142.jpg" alt='COL018112-shutterstock_2036701406' title='COL018112-shutterstock_2036701406'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_659f img{ width:100%; max-width: 350px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><div class="npi"><h3><a href="/blog/behindthebench/omicron-surveillance-using-sanger-or-next-generation-sequencing-to-detect-and-confirm-the-variant/"> Omicron surveillance: Using Sanger or Next Generation Sequencing...</a></h3><p><a href="/blog/behindthebench/omicron-surveillance-using-sanger-or-next-generation-sequencing-to-detect-and-confirm-the-variant/">The SARS-CoV-2 virus continues to evolve as the pendemic pro... by Behind The Bench Staff / 12.09.2021</a></p><h3><a href="/sg/en/home/global/forms/life-science/tsb-series.html?icid=LSG-LPD-HP-NewProducts-WB312690-EMEA"></a><a href="/blog/behindthebench/omicron-surveillance-using-sanger-or-next-generation-sequencing-to-detect-and-confirm-the-variant/">Read More ›</a></h3></div></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2> Why use Sanger sequencing in your SARS-CoV-2 research? </h2></div><div class="text parbase section"><div> Sanger sequencing can complement your laboratory’s techniques and lead to more efficient studies. </div><ul><li>Since being developed in 1977, Sanger sequencing has been the most widely used DNA sequencing method for the past 40 years and remains in wide-spread use around the world<b></b></li><li>Sanger method remains in wide use for smaller-scale projects and for confirmation of NGS results<b></b></li><li>Smaller inquiries with more specific goals can benefit from more focused, less expensive laboratory procedures<b></b></li></ul></div><div class="accordion section"><div class="accordion" id="d53b7a08e9a94558bc7f925a265f97b0" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d53b7a08e9a94558bc7f925a265f97b0" href="#benefits"><span class="caret"></span> The benefits of Sanger sequencing </a></div><div class="accordion-body collapse " id="benefits"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>Sanger sequencing is the gold standard for sequencing single genes, confirming gene variants, detecting repeat sequences, copy number variation, and single nucleotide changes. Sanger sequencing is perfect for:</p><div class="table-component-wrapper"><table cellspacing="0" id="table--903528338"><tbody><tr><td><ul><li>Sequencing single genes and single nucleotide variants</li><li>Targeted sequencing of 100 amplicons or less</li><li>Sequencing up to 96 samples at a time, without barcoding</li><li>NGS confirmation</li><li>High GC-rich sequences</li><li>Microbial identification</li><li>Microsatellite or STR analysis</li><li>Plasmid sequencing</li></ul></td><td><div><img src="/content/dam/LifeTech/global/life-sciences/sequencing/Sanger%20Sequencing/images/showcase/gold-standard.png"><br></div></td></tr></tbody></table></div><div> Learn more: </div><ul><li><a href="/sg/en/home/global/forms/sanger-sequencing-guide-download-form.html">Sanger sequencing guide</a></li><li><a href="/sg/en/home/global/forms/life-science/fragment-analysis-guide.html">Fragment analysis guide </a></li></ul></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d53b7a08e9a94558bc7f925a265f97b0" href="#NGS"><span class="caret"></span> Sanger sequencing or NGS? </a></div><div class="accordion-body collapse " id="NGS"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>While NGS technologies are common in research labs due to higher throughput capabilities, Sanger sequencing offers a cost-effective solution for your research needs.  It does not require expensive equipment and can generate high quality data even for low viral titer samples that do not yield high genome coverage for some NGS technologies.<br></p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#d53b7a08e9a94558bc7f925a265f97b0').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#d53b7a08e9a94558bc7f925a265f97b0').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="heading section"><h2> How can Sanger sequencing and fragment analysis be used in your SARS-CoV-2 research? </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_ac4e"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.full.high.jpg/1660060916367.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.full.high.jpg/1660060916367.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.320.high.jpg/1660060916367.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.480.high.jpg/1660060916367.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.320.medium.jpg/1660060916367.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.620.high.jpg/1660060916367.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.320.low.jpg/1660060916367.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_ac4e/foregroundimg.img.480.medium.jpg/1660060916367.jpg" alt='Sanger_sequencing_SARS-CoV-2 identification' title='Sanger_sequencing_SARS-CoV-2 identification'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_ac4e img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_ac4e .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_ac4e .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><p>As you work to understand SARS-CoV-2 to rapidly identify future treatment options and develop possible vaccine targets, our comprehensive portfolio of Sanger sequencing and fragment analysis research solutions is here to help advance your research.</p><p>In the context of an outbreak, where high numbers of samples need to be processed quickly and accurately, Sanger sequencing and fragment analysis can significantly help you to get answers quickly.</p></div></div></div></div><div style="clear:both"></div><div class="accordion section"><div class="accordion" id="85ffe99e7c4847fe9860880e9d3db2c5" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading active'><a class="accordion-toggle" data-toggle="collapse" data-parent="#85ffe99e7c4847fe9860880e9d3db2c5" href="#identification"><span class="caret"></span> Virus identification </a></div><div class="accordion-body collapse in" id="identification"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div container-id="cq-colctrl-lt1" comp-id="A1"><div class="text parbase section"><p>At the start of the SARS-CoV-2 outbreak, Sanger sequencing was used by several labs to confirm the causative pathogen as being SARS-CoV-2 <sup>1-4</sup>. Additionally, by analyzing these genomes and comparing to other known coronaviruses, it was determined that the virus originated in an animal reservoir that evolved from bats, with a likely intermediate animal host <sup>5,6</sup>.</p></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"><div container-id="cq-colctrl-lt1" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsysimage_cbf9"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.full.high.png/1660060916630.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.full.high.png/1660060916630.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.320.high.png/1660060916630.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.480.high.png/1660060916630.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.320.medium.png/1660060916630.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.620.high.png/1660060916630.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.320.low.png/1660060916630.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/image_cbf9/foregroundimg.img.480.medium.png/1660060916630.png" alt='Sanger_sequencing_virus_research' title='Sanger_sequencing_virus_research'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsysimage_cbf9 img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsysimage_cbf9 .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsysimage_cbf9 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><div> Robust and cost-effective Sanger sequencing and fragment analysis enable you to: </div><ul><li>Quickly identify and distinguish SARS-CoV-2 from other respiratory viruses <sup>1-3</sup><br></li><li>Confirm SARS-CoV-2 S gene 69/70 deletion variant (<a href="https://assets.thermofisher.com/TFS-Assets/GSD/brochures/sequencing-sars-cov-2-s-gene-69-70del-protocol.pdf" target="_blank">download protocol</a>)</li><li>Confirm SARS-CoV-2 B.1.1.7 and B.1.351 strain lineages (<a href="http://assets.thermofisher.com/TFS-Assets/GSD/brochures/protocol_Sanger_sequencing_SARS-CoV-2_B117_B1351_brochure.pdf" target="_blank">download protocol</a>)</li><li>Quickly sequence the entire SARS-CoV-2 spike (S) gene (<a href="https://assets.thermofisher.com/TFS-Assets/GSD/brochures/sequencing-sars-cov-2-spike-gene-protocol.pdf" target="_blank">download protocol</a>)</li><li>Generate gap filling and assembly sequence to aid with full genome NGS virus sequencing <sup>1-6</sup></li><li>Confirm sequence variations identified from full genome NGS data <sup>7</sup></li><li>Utilize a nested PCR approach with full genome sequencing for samples with lower viral load <sup>8</sup></li></ul></div><div class="heading section"><h3> What else can Sanger sequencing and fragment analysis do? </h3></div><div class="text parbase section"><div class="table-component-wrapper"><table cellspacing="0" cellpadding="0" class="rte-table" id="table-347905759"><tbody><tr><th width="400"><b>RT-PCR/qPCR result confirmation</b></th><th><b>Rapid testing for multiple targets</b></th></tr><tr><td width="306" valign="top"><a href="/us/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-workflow.html">Sanger sequencing</a> is being used to confirm RT-PCR/qPCR results and provide confidence in distinguishing SARS-CoV-2 from other respiratory pathogens. In a simplified workflow, PCR product is purified and sequencing primers bind to known sequences in the target region, allowing the sequence to be extended using capillary electrophoresis (CE) <sup>1,3</sup>.<b></b></td><td width="426" valign="top">Multiplexed qPCR solutions test for small numbers of pathogens, and its relatively small capacity can limit throughput when large numbers of targets or pathogens need to be detected. <a href="/us/en/home/life-science/sequencing/fragment-analysis/fragment-analysis-fundamentals.html">Fragment analysis by CE</a> can be used to test multiple pathogens associated with different syndromes in a single sample <sup>9</sup>. For example, a respiratory multiplex panel can detect viral and bacterial pathogens to help rule in/out common pathogens as the cause of infection.</td></tr></tbody></table></div></div><div class="text parbase section"><p>Learn about a simple method for detecting SARS-CoV-2 viral sequences through fragment analysis-based target multiplexing solutions:</p></div><div class="well-cmp section"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsyswell_a865uiparimage_557d"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.full.high.png/1660060916948.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.full.high.png/1660060916948.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.320.high.png/1660060916948.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.480.high.png/1660060916948.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.320.medium.png/1660060916948.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.620.high.png/1660060916948.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.320.low.png/1660060916948.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_d865/itemParsys/well_a865/uipar/image_557d/foregroundimg.img.480.medium.png/1660060916948.png" alt='SARS-CoV-2_detection_application' title='SARS-CoV-2_detection_application'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsyswell_a865uiparimage_557d img{ width:100%; max-width: 276px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsyswell_a865uiparimage_557d .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_d865itemParsyswell_a865uiparimage_557d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><ul><li><a href="/sg/en/home/global/forms/life-science/fragment-analysis-sars-cov-2.html">Download application note: Multiplexed target fragment analysis for detection of viral pathogens including SARS-CoV-2</a></li><li><a href="https://assets.thermofisher.com/TFS-Assets/GSD/Scientific%C2%A0Guides/detection-sarscov2-fragment-analysis-protocol.pdf">Download protocol: Detection of SARS-CoV-2 using fragment analysis</a></li><li><a href="http://assets.thermofisher.com/TFS-Assets/GSD/Reference-Materials/covid-vic-protocol.zip">Download panels, bins, analysis methods, and size standards needed to analyze the files</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#85ffe99e7c4847fe9860880e9d3db2c5" href="#epidemiological"><span class="caret"></span> Epidemiological surveillance research </a></div><div class="accordion-body collapse " id="epidemiological"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div container-id="cq-colctrl-lt1" comp-id="A1"><div class="text parbase section"><p>Low-cost Sanger sequencing is considered the <a href="/us/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-workflow.html">gold standard sequencing method</a> for confirming the sequence of specific genes, including those <a href="/us/en/home/life-science/sequencing/sanger-sequencing/applications/next-generation-sequencing-validation.html">already sequenced through NGS</a>. It can be used to:</p></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"><div container-id="cq-colctrl-lt1" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_810aitemParsysimage_f2e3"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.full.high.png/1660060917298.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.full.high.png/1660060917298.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.320.high.png/1660060917298.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.480.high.png/1660060917298.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.320.medium.png/1660060917298.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.620.high.png/1660060917298.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.320.low.png/1660060917298.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_810a/itemParsys/image_f2e3/foregroundimg.img.480.medium.png/1660060917298.png" alt='Sanger_sequencing_epidemiological_surveillance_research' title='Sanger_sequencing_epidemiological_surveillance_research'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_810aitemParsysimage_f2e3 img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_810aitemParsysimage_f2e3 .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_810aitemParsysimage_f2e3 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><ul><li>Sequence and confirm variants of different SARS-CoV-2 genes within clusters of symptomatic and asymptomatic samples <sup>10-12</sup> and characterize the transmission patterns and genetic evolution of this virus among different populations <sup>13, 4</sup> </li><li>Confirm the origin and cross-species transmission of different coronaviruses, which is important in understanding potential sources of zoonotic transmission <sup>15-17</sup></li><li>Carry out full virus genome sequencing for one-off samples in locations without access to NGS sequencing or for low virus titer samples <sup>8,11</sup></li></ul><p>An understanding of the type and subtype of viruses is essential for effective crisis response. Surveillance also plays a role in understanding cross-species transmission of the virus and its spatial spread over evolutionary time and at human-wildlife interfaces.  Monitoring helps detect the presence of different variants of the virus obtained from samples with different degrees of symptoms, including asymptomatic samples.<b></b></p></div><div class="heading section"><h3> A potential early warning system for SARS-CoV-2 </h3></div><div class="text parbase section"><p>Identification of SARS-CoV-2 in wastewater can provide near real-time information about virus spread and could be an important approach for monitoring the virus spread <sup>18,19</sup>. This method, known as wastewater-based epidemiology (WBE), may be able to act as an early warning system for infectious disease outbreaks in general, and in the immediate future could help predict the second wave of SARS-CoV-2 infections.  Multiple researchers around the world have used Sanger sequencing to confirm the identify of SARS-CoV-2 in wastewater following viral identification by RT-PCR or qPCR <sup>20-22</sup>.</p><p>Read more: <a href="/blog/behindthebench/sars-cov-2-in-wastewater-a-potential-early-warning-method/">SARS-COV-2 in Wastewater: A Potential Early Warning Method</a><br></p></div><div class="heading section"><h3> A role for the pharyngeal microbiome in understanding variability of SARS-CoV-2 infection susceptibility </h3></div><div class="text parbase section"><p>Research indicates that the pharyngeal microbiota (PM) may influence susceptibility to different respiratory viral infections <sup>23,24</sup> making it possible that it may be important in understanding the epidemiology of SARS-CoV-2.  Fragment analysis by CE can be used identify microbial signatures associated with different disease phenotypes <sup>25</sup>. It relies on amplification of 16S-23S rRNA interspace (IS) regions in bacteria to produce PCR fragments that vary in length and frequency depending upon bacterial species. Each PCR fragment can be separated and detected on a genetic analyzer. The resulting fragment patterns are compared to a curated database to determine the species present in the sample <sup>25</sup>.</p><p>Read more: <a href="/blog/behindthebench/could-differences-in-microbiota-explain-variability-in-sars-cov-2-susceptibility/">Could Differences in Microbiota Explain Variability in SARS-CoV-2 Susceptibility? </a></p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#85ffe99e7c4847fe9860880e9d3db2c5" href="#therapeutic"><span class="caret"></span> Vaccine and therapeutic research </a></div><div class="accordion-body collapse " id="therapeutic"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div container-id="cq-colctrl-lt1" comp-id="A1"><div class="text parbase section"><p>There is an urgent need to develop safe and specific anti-viral treatments and/or vaccines for SARS-CoV-2. Vaccines provide a long-term solution to reduce the risk of infection by training the immune system to respond quickly and effectively. Therefore, it is critical to select a vaccine approach and begin with basic lab research to identify the pathogen and antigen candidates that could cause an immune response.</p></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"><div container-id="cq-colctrl-lt1" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_7f7fitemParsysimage_379f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.full.high.png/1660060917576.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.full.high.png/1660060917576.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.320.high.png/1660060917576.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.480.high.png/1660060917576.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.320.medium.png/1660060917576.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.620.high.png/1660060917576.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.320.low.png/1660060917576.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_700a/itemspar/accordionitem_7f7f/itemParsys/image_379f/foregroundimg.img.480.medium.png/1660060917576.png" alt='Sanger_sequencung_vaccine_therapeutic_research' title='Sanger_sequencung_vaccine_therapeutic_research'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_7f7fitemParsysimage_379f img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_7f7fitemParsysimage_379f .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysaccordion_700aitemsparaccordionitem_7f7fitemParsysimage_379f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div></div><div style="clear:both"></div><div class="heading section"><h3> Sanger sequencing: the ideal approach </h3></div><div class="text parbase section"><p>The simple, fast, cost-effective <a href="/us/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-workflow.html">Sanger sequencing workflow</a> is ideal at different steps of the <a href="/us/en/home/biotech-lab-solutions/therapeutics-research-development-solutions/vaccine-research-development-tools.html">vaccine development workflow</a>:</p><ul><li>In target discovery, it is being used to identify the virus and its variants</li><li>It can help with optimizing of the sequence of the mRNA vaccine to maximize protein expression <sup>26</sup></li><li>It can be used to sequence and characterize the neutralizing antibodies generated as a response to the virus, which could potentially have therapeutic or prophylactic utility <sup>27-29</sup></li></ul></div><div class="heading section"><h3> The importance of human cell line authenticity </h3></div><div class="text parbase section"><p>Vaccine research is often performed with cultured human cells obtained from major repositories or fellow researchers. An estimated 15–20% of the time, cells used in experiments have been misidentified or cross-contaminated with another cell line. Although major repositories now authenticate cell line identity, many are calling for all researchers to test and authenticate cell line identity using standard genotyping techniques like <a href="/us/en/home/life-science/sequencing/fragment-analysis/short-tandem-repeat-analysis-human-sample-identification.html">Short Tandem Repeat (STR) genotyping with CE</a>.</p><p>We offer products to authenticate cell line identity:</p><ul><li>Applied Biosystems <a href="/us/en/home/life-science/sequencing/fragment-analysis/short-tandem-repeat-analysis-human-sample-identification.html">CLA Identifiler Plus and Direct kits</a> are optimized to analyze 16 highly variant human STRs with amplification time of under three hours<br></li><li>Applied Biosystems <a href="/us/en/home/life-science/sequencing/sanger-sequencing/sanger-dna-sequencing/sanger-sequencing-data-analysis.html">GeneMapper Software 6</a> and the cloud-based <a href="/us/en/home/cloud/all-analysis-modules/sanger-analysis-modules.html">microsatellite analysis (MSA) software</a> solutions facilitate analysis of STRs by making use of pre-established allelic ladders and sizing bin sets for the various STR alleles covered by the Identifiler kits</li></ul></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#85ffe99e7c4847fe9860880e9d3db2c5').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#85ffe99e7c4847fe9860880e9d3db2c5').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> The Role of Capillary Electrophoresis (CE) in Drug Development </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_7600uiparimage_a32f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.full.high.jpg/1660060917850.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.full.high.jpg/1660060917850.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.320.high.jpg/1660060917850.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.480.high.jpg/1660060917850.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.320.medium.jpg/1660060917850.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.620.high.jpg/1660060917850.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.320.low.jpg/1660060917850.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_7600/uipar/image_a32f/foregroundimg.img.480.medium.jpg/1660060917850.jpg" alt='Sanger sequencing_infectious_disease_research' title='Sanger sequencing_infectious_disease_research'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_7600uiparimage_a32f img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_7600uiparimage_a32f .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_7600uiparimage_a32f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><p><b>Emerging viruses pose a great challenge to global health care and economic systems. Given their pandemic potential, as witnessed in the case of the SARS-CoV-2 virus, rapid development and screening of drugs is needed to combat and contain the infection spread.</b> <br></p><p><a href="/blog/behindthebench/the-role-of-capillary-electrophoresis-ce-in-drug-development/"><b>Read Blog ›</b></a></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> How are other scientists using Sanger sequencing in their infectious disease research? </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_a32f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.full.high.jpg/1660060918085.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.full.high.jpg/1660060918085.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.320.high.jpg/1660060918085.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.480.high.jpg/1660060918085.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.320.medium.jpg/1660060918085.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.620.high.jpg/1660060918085.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.320.low.jpg/1660060918085.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_a32f/foregroundimg.img.480.medium.jpg/1660060918085.jpg" alt='Sanger sequencing_infectious_disease_research' title='Sanger sequencing_infectious_disease_research'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_a32f img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_a32f .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_a32f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><blockquote><b>“Sanger sequencing can accomplish what some other new platforms can’t, especially when you want to look at minor allele frequency haplotypes from contagious viral genes that might not get captured in some of the higher throughout platforms.” </b>     <br><br> Dr. Lillian Seu <br> Center for Infectious Disease Control, Zambia </blockquote><p><a href="/blog/behindthebench/using-sanger-sequencing-to-detect-drug-resistance-mutations-in-hiv-in-zambia/"><b>Read how Sanger sequencing was used to help detect drug resistance mutation in HIV research in Zambia</b></a></p></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_8cc7"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.full.high.jpg/1660060918159.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.full.high.jpg/1660060918159.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.320.high.jpg/1660060918159.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.480.high.jpg/1660060918159.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.320.medium.jpg/1660060918159.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.620.high.jpg/1660060918159.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.320.low.jpg/1660060918159.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_def8/uipar/image_8cc7/foregroundimg.img.480.medium.jpg/1660060918159.jpg" alt='Sanger_sequencing_disease_research' title='Sanger_sequencing_disease_research'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_8cc7 img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_8cc7 .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_def8uiparimage_8cc7 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><blockquote><b>“The high-precision and sensitivity of Sanger sequencing make it irreplaceable in confirming point mutations, determining the position of cloned DNA, sequencing short internal gaps, or missing data at the genome termini.”</b>  <br><br> Dr. Iryna Goraichuk <br> USDA-ARS Exotic and Emerging Avian Viral Unit </blockquote><p><a href="/blog/behindthebench/new-avian-viruses-discovered-with-the-help-of-sanger-sequencing/"><b>Read how new avian viruses have been discovered with the help of Sanger sequencing</b></a><b></b></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="well-cmp section"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Get SARS-CoV-2 research results faster with a single-cartridge multi-application genetic analyzer </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_e860uiparimage_fced"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.full.high.jpg/1660060918308.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.full.high.jpg/1660060918308.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.320.high.jpg/1660060918308.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.480.high.jpg/1660060918308.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.320.medium.jpg/1660060918308.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.620.high.jpg/1660060918308.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.320.low.jpg/1660060918308.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_e860/uipar/image_fced/foregroundimg.img.480.medium.jpg/1660060918308.jpg" alt='Sanger_sequencing_SARS-CoV-2_SeqStudio' title='Sanger_sequencing_SARS-CoV-2_SeqStudio'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_e860uiparimage_fced img{ width:100%; max-width: 270px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_e860uiparimage_fced .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_e860uiparimage_fced .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><ul><li>Run both Sanger sequencing and fragment analysis samples on the same plate at the same time</li><li>Reduce setup time</li><li>Maximize benchtop space</li><li>Access, analyze, and share data anywhere, anytime with Connect</li></ul><p><a href="/us/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-technology-accessories/seqstudio-genetic-analyzer.html"><b>Learn more ›</b></a><b></b></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="heading section"><h2> Sanger sequencing workflow </h2></div><div class="text parbase section"><p>Our simple and fast <a href="/us/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-workflow.html">Sanger sequencing workflow</a> can be completed in less than one workday, from sample to answer. We offer products that support many steps of our recommended workflow, from PCR amplification to data analysis.</p></div><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_53c"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.320.low.jpg/1660060918403.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.320.medium.jpg/1660060918403.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.480.medium.jpg/1660060918403.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.620.high.jpg/1660060918403.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.full.high.jpg/1660060918403.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.320.high.jpg/1660060918403.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.480.high.jpg/1660060918403.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.320.medium.jpg/1660060918403.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.620.high.jpg/1660060918403.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.320.low.jpg/1660060918403.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_53c/foregroundimg.img.480.medium.jpg/1660060918403.jpg" alt='Sanger_sequencing_workflow' title='Sanger_sequencing_workflow'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_53c img{ width:100%; max-width: 960px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_53c .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_53c .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="accordion section"><div class="accordion" id="71db570cf7784052a71d0a8d14e95470" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading active'><a class="accordion-toggle" data-toggle="collapse" data-parent="#71db570cf7784052a71d0a8d14e95470" href="#Ordering"><span class="caret"></span> Ordering information </a></div><div class="accordion-body collapse in" id="Ordering"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="regionalwrapper parbase section"></div><div class="productlist parbase section"><div id="skulist" style="display:none">4458687,A35644,A38073,14000012,75001.1.ML,4337455,4337450,4376486,4440462,A38876,A30835,4474978 </div><div id='ltajax-4950184718238703463'></div><script type="text/javascript">(function() {$('#ltajax-4950184718238703463').load('/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordion_dc3e/itemspar/accordionitem_8758/itemParsys/productlist_412f.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#71db570cf7784052a71d0a8d14e95470').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#71db570cf7784052a71d0a8d14e95470').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="heading section"><h2> Fragment analysis workflow </h2></div><div class="text parbase section"><p>From straightforward sample preparation to peak analysis, we offer a wide range of products and services to simplify each step of the <a href="/us/en/home/life-science/sequencing/fragment-analysis/fragment-analysis-fundamentals.html">fragment analysis workflow</a>.</p></div><div class="image parbase section"><div id="cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_bce2"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.320.low.jpg/1660060918685.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.320.medium.jpg/1660060918685.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.480.medium.jpg/1660060918685.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.620.high.jpg/1660060918685.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.full.high.jpg/1660060918685.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.320.high.jpg/1660060918685.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.480.high.jpg/1660060918685.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.320.medium.jpg/1660060918685.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.620.high.jpg/1660060918685.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.320.low.jpg/1660060918685.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/image_bce2/foregroundimg.img.480.medium.jpg/1660060918685.jpg" alt='Fragment_analysis_worklow' title='Fragment_analysis_worklow'></picture><style> #cq-image-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_bce2 img{ width:100%; max-width: 950px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_bce2 .image-contain" + "," + "#cq-image-jsp-"+"dbedb995-b68c-4273-b8ac-248879ab1004MainParsysimage_bce2 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#71db570cf7784052a71d0a8d14e95470" href="#Ordering"><span class="caret"></span> Ordering information </a></div><div class="accordion-body collapse " id="Ordering"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">14000012,4311320,4345833,4408399,A35644,A30468,A38888,A29763,450056 </div><div id='ltajax-7809735195327048814'></div><script type="text/javascript">(function() {$('#ltajax-7809735195327048814').load('/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/accordionitem_9c6f/itemParsys/productlist_412f.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="accordion section"><div class="accordion" id="eef381b9718946e7878d5b136659fafe" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#eef381b9718946e7878d5b136659fafe" href="#references"><span class="caret"></span> References </a></div><div class="accordion-body collapse " id="references"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>Sanger sequencing and fragment analysis protocols are referenced in the publications below:</p><ol><li>Chan, JFW., et. al. (2020) A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet; 395: 514–23 (<a href="https://doi.org/10.1016/S0140-6736(20)30154-9">https://doi.org/10.1016/S0140-6736(20)30154-9</a>)</li><li>Zhu, N., et. al. (2020) A Novel Coronavirus from Patients with Pneumonia in China, 2019. N Engl J Med 382;8 (<a href="https://doi.org/10.1056/nejmoa2001017">https://doi.org/10.1056/nejmoa2001017</a>)</li><li>Caly, L., et. al. (2020) Isolation and rapid sharing of the 2019 novel coronavirus (SARS‐CoV‐2) from the first patient diagnosed with COVID‐19 in Australia. Med J Aust 2020; 212 (10): 459-462. (<a href="https://doi.org/10.5694/mja2.50569">https://doi.org/10.5694/mja2.50569</a>)</li><li>Lam, LT., et. al. (2020) “Whole-genome sequencing and de novo assembly of a 2019 novel Coronavirus (sars-cov-2) strain isolated in Vietnam. Preprint at bioRxiv doi: (<a href="https://doi.org/10.1101/2020.06.12.149377">https://doi.org/10.1101/2020.06.12.149377</a>)</li><li>Zhou, P., et. al. (2020) A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature (579) (<a href="https://doi.org/10.1038/s41586-020-2012-7">https://doi.org/10.1038/s41586-020-2012-7</a>)</li><li>Lu R,. et.al. (2020) Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet: 395: 565-574 (<a href="https://doi.org/10.1016/S0140-6736(20)30251-8">https://doi.org/10.1016/S0140-6736(20)30251-8</a>)</li><li>Artesi, M., et al. (2020) A recurrent mutation at position 26,340 of SARS-CoV-2 is associated with failure of 2 the E-gene qRT-PCR utilized in a commercial dual-target diagnostic assay. J. Clin. Microbiol. doi:10.1128/JCM.01598-20 (<a href="https://doi.org/10.1128/jcm.01598-20">https://doi.org/10.1128/jcm.01598-20</a>)</li><li>Paden CR, et al., (2020). Rapid, sensitive, full-genome sequencing of severe acute respiratory syndrome coronavirus 2. Emerg Infect Dis. 26 (10) 3201. (<a href="https://doi.org/10.3201/eid2610.201800">https://doi.org/10.3201/eid2610.201800</a>)</li><li>Gomez, J., et al. (2020) “Capillary electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2”. J. Virology 284 113937 (<a href="https://doi.org/10.1016/j.jviromet.2020.113937">https://doi.org/10.1016/j.jviromet.2020.113937</a>)</li><li>Yuan, Y,. et al. (2020) “Molecular Epidemiology of SARS-CoV-2 Clusters Caused by Asymptomatic Cases in Anhui Province, China” Research Square preprint (<a href="https://dx.doi.org/10.21203/rs.3.rs-29833/v1">https://dx.doi.org/10.21203/rs.3.rs-29833/v1</a>)</li><li>Holland, LA., et al. (2020) “An 81 nucleotide deletion in SARS-1 CoV-2 ORF7a identified from sentinel surveillance in Arizona (Jan-Mar 2020)” J. Virology (<a href="https://doi.org/10.1128/jvi.00711-20">https://doi.org/10.1128/jvi.00711-20</a>)</li><li>Liu, Z., et al. (2020) “Identification of common deletions in 1 the spike protein of SARS-CoV-2”. J. Virology (<a href="/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research.html">https:// doi:10.1128/JVI.00790-20</a>)</li><li>Zhang, Z-B., et al. (2020) “Genomic surveillance of a resurgence of COVID-19 in Guangzhou, China”. Research Square preprint (<a href="https://doi.org/10.21203/rs.3.rs-35869/v1">https://doi.org/10.21203/rs.3.rs-35869/v1</a>)</li><li>Wang, Y., et al. (2020) “Intra-host Variation and 1 Evolutionary Dynamics of SARS-CoV-2 Population in COVID-19 Patients”. Preprint at bioRxiv doi: (<a href="https://doi.org/10.1101/2020.05.20.103549">https://doi.org/10.1101/2020.05.20.103549</a>)</li><li>Valitutto, M.T., et al. (2020) “Detection of novel coronaviruses in bats in Myanmar,” PLoS One 15(4): e0230802.(<a href="https://doi.org/10.1371/journal.pone.0230802">https://doi.org/10.1371/journal.pone.0230802</a>)</li><li>Latinne A., et al. (2020) Origin and cross-species transmission of bat coronaviruses in China. (bioRxiv preprint doi: <a href="https://doi.org/10.1101/2020.05.31.116061">https://doi.org/10.1101/2020.05.31.116061</a>)</li><li>Huong, N.Q., et al (2020) “Coronavirus testing indicates transmission risk increases along 2 wildlife supply chains for human consumption in Viet Nam, 2013-2014 (bioRxiv preprint doi: <a href="https://doi.org/10.1101/2020.06.05.098590">https://doi.org/10.1101/2020.06.05.098590</a>.)</li><li>Kitajima, M. et al. (2020) “SARS-CoV-2 in wastewater: State of the knowledge and research needs”. Science of the Total Environment 739,  139076. (<a href="https://doi.org/10.1016/j.scitotenv.2020.139076">https://doi.org/10.1016/j.scitotenv.2020.139076</a>)</li><li>Carducci Aet al., (2020) Making Waves: Coronavirus detection, presence and persistence in the water environment: State of the art and knowledge needs for public health. Water Res. 2020;179:115907. (<a href="https://doi.org/10.1016/j.watres.2020.115907">https://doi.org/10.1016/j.watres.2020.115907</a>)</li><li>Ahmed W et al. (2020) “First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for wastewater surveillance of COVID-19 in the community”. <i>Sci Total Environ </i>DOI:(<i></i><a href="https://doi.org/10.1016/j.scitotenv.2020.138764">https://doi.org/10.1016/j.scitotenv.2020.138764</a>)</li><li>Wu, F.Q. et al. (2020) “SARS-CoV-2 titers in wastewater are higher than expected from clinically confirmed cases.” <i>medRxiv preprint</i>,doi: (<a href="https://doi.org/10.1101/2020.04.05.20051540">https://doi.org/10.1101/2020.04.05.20051540</a>)</li><li>Nemudryi, A. et al, (2020) “Temporal detection and phylogenetic assessment of SARS-CoV-2 in municipal wastewater”, Preprint at medRxiv (<a href="https://doi.org/10.1101/2020.04.15.20066746">https://doi.org/10.1101/2020.04.15.20066746</a>)</li><li>De Steenhuijsen Piters WAA, et al. (2016) “Nasopharyngeal microbiota, host transcriptome, and disease severity in children with respiratory syncytial virus infection”. Am J Respir Crit Care Med; 194: 1104–15. (<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114450/">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114450/</a>)</li><li>Tsang TK, et al. (2019) “Association Between the Respiratory Microbiome and Susceptibility to Influenza Virus Infection.” Clin Infect Dis; 48109: 1–9. (<a href="https://pubmed.ncbi.nlm.nih.gov/31562814/">https://pubmed.ncbi.nlm.nih.gov/31562814/</a>)</li><li>Budding A., et al., (2020)  “An Age Dependent Pharyngeal Microbiota Signature Associated with SARS-CoV-2 Infection (4/21/2020).” Preprint at (<a href="http://dx.doi.org/10.2139/ssrn.3582780">http://dx.doi.org/10.2139/ssrn.3582780</a>)</li><li>Zeng, C., et al. (2020) “Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo” Preprint at bioRxiv doi: (<a href="https://doi.org/10.1101/2020.04.01.019877">https://doi.org/10.1101/2020.04.01.019877</a>).</li><li>Klimstra, W.B., et al. (2020) “SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected, hospitalized COVID-19 patients”. Preprint at bioRxiv doi:(<a href="https://doi.org/10.1101/2020.06.19.154930">https://doi.org/10.1101/2020.06.19.154930</a>)</li><li>Seydoux E., et al. (2020) “Characterization of neutralizing antibodies 1 from a SARS-CoV-2 infected individual”.  Preprint at bioRxiv doi: (<a href="https://doi.org/10.1101/2020.05.12.091298">https://doi.org/10.1101/2020.05.12.091298</a>)</li><li>Noy-Porat, T., et al. (2020) “Tiger team: a panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopes”.  Preprint at bioRxiv doi: (<a href="https://doi.org/10.1101/2020.05.20.106609">https://doi.org/10.1101/2020.05.20.106609</a>)</li></ol></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#eef381b9718946e7878d5b136659fafe').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#eef381b9718946e7878d5b136659fafe').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_6ab1"><a href="/sg/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-workflow.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.full.high.jpg/1660060918828.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.full.high.jpg/1660060918828.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.320.high.jpg/1660060918828.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.480.high.jpg/1660060918828.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.320.medium.jpg/1660060918828.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.620.high.jpg/1660060918828.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.320.low.jpg/1660060918828.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_6ab1/image.img.480.medium.jpg/1660060918828.jpg" alt='Learn about the Sanger sequencing workflow' title='Learn about the Sanger sequencing workflow'></picture></div><style> #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_6ab1 { max-width: 100%; } #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_6ab1 img { width: 100%; max-width: 85px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><b><a adhocenable="false" href="/sg/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-workflow.html">Learn about the Sanger sequencing workflow</a></b></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_d8b8"><a href="/sg/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-technology-accessories.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.full.high.jpg/1660060918892.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.full.high.jpg/1660060918892.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.320.high.jpg/1660060918892.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.480.high.jpg/1660060918892.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.320.medium.jpg/1660060918892.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.620.high.jpg/1660060918892.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.320.low.jpg/1660060918892.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_d8b8/image.img.480.medium.jpg/1660060918892.jpg" alt='Instruments for Sanger sequencing and fragment analysis' title='Instruments for Sanger sequencing and fragment analysis'></picture></div><style> #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_d8b8 { max-width: 100%; } #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_d8b8 img { width: 100%; max-width: 85px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><b><a adhocenable="false" href="/sg/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-technology-accessories.html">Instruments for Sanger sequencing and fragment analysis</a></b></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_750f"><a href="/sg/en/home/cloud/all-analysis-modules/sanger-analysis-modules.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.full.high.jpg/1660060918936.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.full.high.jpg/1660060918936.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.320.high.jpg/1660060918936.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.480.high.jpg/1660060918936.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.320.medium.jpg/1660060918936.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.620.high.jpg/1660060918936.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.320.low.jpg/1660060918936.jpg,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/textimage_750f/image.img.480.medium.jpg/1660060918936.jpg" alt='Sanger Analysis Modules' title='Sanger Analysis Modules'></picture></div><style> #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_750f { max-width: 100%; } #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsystextimage_750f img { width: 100%; max-width: 82px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><b><a adhocenable="false" href="/sg/en/home/life-science/sequencing/sanger-sequencing/sanger-dna-sequencing/sanger-sequencing-data-analysis.html">Sanger sequencing and fragment analysis software</a></b></p></div></div><div class="clearfix"></div></div></div></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_95b3uipartextimage_2f08"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.full.high.png/1660060919039.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.full.high.png/1660060919039.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.320.high.png/1660060919039.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.480.high.png/1660060919039.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.320.medium.png/1660060919039.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.620.high.png/1660060919039.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.320.low.png/1660060919039.png,https://www.thermofisher.com/sg/en/home/life-science/sequencing/sanger-sequencing/applications/sars-cov-2-research/jcr:content/MainParsys/well_95b3/uipar/textimage_2f08/image.img.480.medium.png/1660060919039.png" alt='Build your knowledge' title='Build your knowledge'></picture></div><style> #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_95b3uipartextimage_2f08 { max-width: 100%; } #cq-textimage-jsp-dbedb995-b68c-4273-b8ac-248879ab1004MainParsyswell_95b3uipartextimage_2f08 img { width: 100%; max-width: 68px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h2>Build your knowledge</h2></div></div><div class="clearfix"></div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><div class="h3"><a href="/sg/en/home/life-science/sequencing/sequencing-education/seq-it-out.html">Seq It Out video series </a></div></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><p>Get bite-sized answers to your everyday Sanger sequencing and fragment analysis questions</p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><div class="h3"><a href="/sg/en/home/life-science/sequencing/sequencing-education.html">Sequencing education center</a></div></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><p>Learn about the history of sequencing and how to pick the right platform for your research needs</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><h2>Resources</h2><ul class="the-icons clearfix"><li><a href="/sg/en/home/global/forms/sanger-sequencing-guide-download-form.html">Sanger Sequencing Guide</a></li><li><a href="/sg/en/home/life-science/sequencing/sanger-sequencing/applications.html">Sanger sequencing applications</a></li><li><a href="/sg/en/home/life-science/sequencing/sanger-sequencing/sanger-sequencing-workflow.html">Sanger sequencing workflow</a></li><li><a href="/sg/en/home/global/forms/life-science/fragment-analysis-guide.html">Fragment Analysis Guide</a></li><li><a href="/sg/en/home/life-science/sequencing/fragment-analysis.html">Fragment analysis applications</a></li><li><a href="/sg/en/home/life-science/sequencing/fragment-analysis/fragment-analysis-fundamentals.html">Fragment analysis workflow</a></li><li><a href="https://learn.thermofisher.com/dnasequencing">Sequencing and fragment analysis training</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><h2>Support</h2><ul class="the-icons clearfix"><li><a href="/us/en/home/technical-resources/technical-reference-library/capillary-electrophoresis-software-support-center.html">Capillary Electrophoresis Software Support Center</a></li><li><a href="/us/en/home/technical-resources/technical-reference-library/capillary-electrophoresis-instruments-support-center.html">Capillary Electrophoresis Instruments Support Center</a></li><li><a href="/us/en/home/technical-resources.html">Technical support</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div><div><div class="MainInheritParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="MainInheritParsys iparsys parsys"><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="text_72be text parbase"><p>For Research Use Only. 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