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href="https://www.reactionbiology.com/wp-content/et-cache/global/et-divi-customizer-global.min.css?ver=1731227809" /> <link rel="stylesheet" id="template-page-section-css" href="https://www.reactionbiology.com/wp-content/themes/Divi-child/css/template-page.css" type="text/css" media="all"><link rel="stylesheet" id="responsive-styles-css" href="https://www.reactionbiology.com/wp-content/themes/Divi-child/css/responsive-styles.css" type="text/css" media="all"><link rel = "stylesheet" type = "text/css" media = "print" href = "https://www.reactionbiology.com/wp-content/themes/Divi-child/css/print-media-styles.css"> <style> .field--name-field-figure img {margin-top:30px;} </style> </head> <body data-rsssl=1 class="datasheet-template-default single single-datasheet postid-3401 page-node-post page-node-type-post et_pb_button_helper_class et_fixed_nav et_show_nav et_primary_nav_dropdown_animation_fade et_secondary_nav_dropdown_animation_fade et_header_style_left et_pb_svg_logo et_pb_footer_columns4 et_cover_background et_pb_gutter windows et_pb_gutters3 et_right_sidebar et_divi_theme et-db"> <main id="main-content" role="main"> <div class="container center"> <article id="post-3401" class="node node--type-datasheet node--promoted color-scheme- node--view-mode-full post-3401 post type-post status-publish format-standard hentry" itemtype="https://schema.org/CreativeWork" itemscope=""> <div id="block-proquinase-page-logo" class="block block-core block-page-title-block"> <div class="block block-core block-page-title-block header-logo"> <a href="https://www.reactionbiology.com/" class="site-logo"> <img src="https://www.reactionbiology.com/wp-content/uploads/2023/06/rb-logo.svg" alt="Reaction Biology" id="logo" data-height-percentage="54" /> </a> </div> </div> <div id="block-proquinase-page-title" class="color-scheme- block block-core block-page-title-block"> <h1 class="page-title"> <span class="field field--name-title field--type-string field--label-hidden"> CYP2J2 Inhibition Assay Service </span> </h1> </div> <div class="node__content"> <div class="entry-content entry-content-servises"> <div class="field field--name-field-subtitle field--type-text-long field--label-hidden field__item"> <p>CYP2J2 for compound screening and profiling via fluorescence-based <a href="/services/adme-safety/cytochrome-p450-assays">cytochrome P450 inhibition assay</a> quantification of enzyme activity.</p> </div> <div class="field field--name-field-properties field--type-entity-reference-revisions field--label-hidden field__items"> <div class="paragraph paragraph--type--datasheet-category-header paragraph--view-mode--default"> <div class="field field--name-field-title field--type-string field--label-hidden field__items"> <div class="field__item">Target Overview</div> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">CAT#</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>CYP2J2</p> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Enzyme</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>CYP2J2 + NADPH-P450 Reductase (microsomes from insect cells infected with baculovirus containing the human CYP2J2 cDNA and rabbit Cytochrome P450 cDNA)</p> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Synonym</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>Cytochrome P450, family 2, subfamily J, member 2</p> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Substrate</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>Fluorescence Substrate Vivid® MOBFC</p> </div> </div> <div class="paragraph paragraph--type--datasheet-category-header paragraph--view-mode--default"> <div class="field field--name-field-title field--type-string field--label-hidden field__items"> <div class="field__item">Assay Properties</div> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Reaction</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>RH + O₂ + NADPH + H⁺ = ROH + H₂O + NADP⁺</p> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Readout</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>Enzymatic activity</p> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Reference Compound IC50s</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <table> <thead> <tr> <th>Compound</th> <th>IC50 (µM)</th> </tr> </thead> <tbody> <tr> <td>Ketoconazole</td> <td>0.43</td> </tr> <tr> <td>Tranylcypromine</td> <td>24</td> </tr> <tr> <td>Terfenadine</td> <td>5.9</td> </tr> </tbody> </table> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Scientific Information</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p><a href="https://www.uniprot.org/uniprotkb/P51589/entry">P51589</a> (https://www.uniprot.org/uniprotkb/P51589/entry)</p> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Screening Location</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>Malvern, PA, USA</p> </div> </div> <div class="paragraph paragraph--type--property-with-two-textfields paragraph--view-mode--default"> <div class="field field--name-field-key field--type-text field--label-hidden field__item">Further Information</div> <div class="field field--name-field-value field--type-text-long field--label-hidden field__item"> <p>More information can be found on our website <a href="https://www.reactionbiology.com/services/adme-safety/cytochrome-p450-assays">CYP450 Inhibition Assay Services</a>.</p> </div> </div> </div> <div class="field field--name-field-figure field--type-entity-reference-revisions field--label-hidden field__items"> <div class="field__item"> <div class="paragraph paragraph--type--datasheet-figure paragraph--view-mode--default"> <div class="field field--name-field-figure-heading field--type-text-long field--label-hidden field__item"> <p>Reference compound IC50 for CYP2J2</p> </div> <div class="field field--name-field-figure-description field--type-text-long field--label-hidden field__item"> <p><img fetchpriority="high" decoding="async" class="alignleft wp-image-3402" src="https://www.reactionbiology.com/wp-content/uploads/2023/07/CYP2J2_malvern.png" alt="" width="550" height="273" srcset="https://www.reactionbiology.com/wp-content/uploads/2023/07/CYP2J2_malvern.png 1090w, https://www.reactionbiology.com/wp-content/uploads/2023/07/CYP2J2_malvern-300x149.png 300w, https://www.reactionbiology.com/wp-content/uploads/2023/07/CYP2J2_malvern-1024x509.png 1024w, https://www.reactionbiology.com/wp-content/uploads/2023/07/CYP2J2_malvern-768x382.png 768w, https://www.reactionbiology.com/wp-content/uploads/2023/07/CYP2J2_malvern-1080x537.png 1080w" sizes="(max-width: 550px) 100vw, 550px" /></p> </div> </div> </div> </div> </div> </div> </article> </div> </main> <footer id="footer" role="contentinfo" class="footer-top"> <div class="container center"> <div class="footer-inner-wrap"> <div class="footer-inner"> <div class="datasheet-footer-slogan"><div class="center">Let's Discover Together.</div></div> <div class="copy"><div class="center"><div class="copy-year">© Nov 2024 <strong>Reaction Biology.</strong> All rights reserved.</div></div></div> <div class="url center">https://www.reactionbiology.com/datasheet/cyp2j2_malvern/</div> </div> <div class="footer-button"> 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