Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae

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Infections With Streptococcus dysgalactiae</span></h1></div> <div class="rendering rendering_researchoutput rendering_researchoutput_associatespersonsclassifiedportal rendering_contributiontojournal rendering_associatespersonsclassifiedportal rendering_contributiontojournal_associatespersonsclassifiedportal"><p class="relations persons"><a rel="Person" href="https://portal.research.lu.se/en/persons/anna-bl%C3%A4ckberg" class="link person"><span>Anna Bläckberg</span></a>, <a rel="Person" href="https://portal.research.lu.se/en/persons/therese-de-neergaard" class="link person"><span>Therese de Neergaard</span></a>, <a rel="Person" href="https://portal.research.lu.se/en/persons/inga-maria-frick" class="link person"><span>Inga Maria Frick</span></a>, <a rel="Person" href="https://portal.research.lu.se/en/persons/pontus-nordenfelt" class="link person"><span>Pontus Nordenfelt</span></a>, <a rel="Person" href="https://portal.research.lu.se/en/persons/rolf-lood" class="link person"><span>Rolf Lood</span></a>, <a rel="Person" href="https://portal.research.lu.se/en/persons/magnus-rasmussen" class="link person"><span>Magnus Rasmussen</span></a></div> <div class="rendering rendering_researchoutput rendering_researchoutput_associatesorganisationsportal rendering_contributiontojournal rendering_associatesorganisationsportal rendering_contributiontojournal_associatesorganisationsportal"><ul class="relations organisations"><li class="division"><a rel="Organisation" href="https://portal.research.lu.se/en/organisations/infection-medicine-bmc" class="link"><span>Infection Medicine (BMC)</span></a></li></ul></div> <div class="rendering rendering_researchoutput rendering_researchoutput_associatesexternalorganisationsportal rendering_contributiontojournal rendering_associatesexternalorganisationsportal rendering_contributiontojournal_associatesexternalorganisationsportal"><ul class="relations organisations"><li><a rel="UEOExternalOrganisation" href="#" class="link"><span>Skåne University Hospital</span></a></li></ul></div> <div class="rendering rendering_researchoutput rendering_researchoutput_publicationcontenttyperendererportalng rendering_contributiontojournal rendering_publicationcontenttyperendererportalng rendering_contributiontojournal_publicationcontenttyperendererportalng"><p class="type"><span class="type_family">Research output<span class="type_family_sep">: </span></span><span class="type_classification_parent">Contribution to journal<span class="type_parent_sep"> › </span></span><span class="type_classification">Article</span><span class="type_classification"><span class="type_parent_sep"> › </span>peer-review</span></p></div> </div> <div class="publication-metrics" aria-label="Publication metrics section"> <div class="metrics-container"> <div class="metrics-wrap metrics-bottom "> <div class="metric metric-plum-x"> <a href="https://plu.mx/plum/a/?doi=10.3389/fmicb.2021.635591" class="plumx-plum-print-popup" data-on-empty="plumXError" data-on-fail="plumXError" data-on-success="plumXSuccess" data-popup="left" data-hide-when-empty="true" data-site="plum" data-badge="false"></a> </div> <div class="metric metric-altmetric"> <div data-badge-popover="top" data-badge-type="donut" data-doi="10.3389/fmicb.2021.635591" data-hide-no-mentions="true" data-link-target='_blank' class="altmetric-embed"></div> </div> </div> </div> </div> </div> </div> <div class="subMenu"> <div class="content-profile-navigation"> <div class="container"> <nav role="navigation"> <ul class="has-overflow-navigation" role="menubar"> <li class="selected" role="none"> <a class="portal_link" role="menuitem" href="/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st"><span> <i class="icon icon-publication" aria-hidden="true"></i> <span class="label">Overview</span> </span></a> </li> <li class="" role="none"> <a class="portal_link" role="menuitem" href="/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st/fingerprints/"><span> <i class="icon icon-fingerprint" aria-hidden="true"></i> <span class="label">Fingerprint</span> </span></a> </li> <li class="" role="none"> <a class="portal_link" role="menuitem" href="/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st/publications/"><span> <i class="icon icon-publications"></i> <span class="label">Research output</span> <span class="count">(2)</span> </span></a> </li> </ul> </nav> </div> </div> </div> </section> <div id="main-content" class="page-section-content page-section-content-publications page-section-content-publications-view no-margin "> <section class="page-section content-relation-section"> <div class="container"> <div class="clearfix"> <div class="content-content publication-content"> <h2 class="subheader">Abstract</h2> <div class="rendering rendering_researchoutput rendering_researchoutput_abstractportal rendering_contributiontojournal rendering_abstractportal rendering_contributiontojournal_abstractportal"><div class="textblock"><p>Introduction: Streptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity. Materials and Methods: Patients with S. dysgalactiae bacteraemia in the county of Skåne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function. Results: Sixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent episodes of bacteraemia. Using ELISA with S. dysgalactiae isolates and mutants, development of IgG antibodies was demonstrated in few patients. Type-specific antibodies were demonstrated in one patient when recombinant M proteins as antigens, were applied. The type-specific serum mediated a small increase in phagocytosis but did not facilitate increased killing of the S. dysgalactiae isolate, carrying that M protein, in blood or by phagocytic cells. Conclusion: S. dysgalactiae bacteraemia sometimes results in increased levels of antibodies to the infecting pathogen. We did not find evidence that these antibodies are effectively opsonising. Apparent failure to produce opsonising antibodies might partially explain why S. dysgalactiae can cause recurrent invasive infections in the same host.</p></div></div> <div class="rendering rendering_researchoutput rendering_researchoutput_detailsportal rendering_contributiontojournal rendering_detailsportal rendering_contributiontojournal_detailsportal"><div class="article"><table class="properties"><tbody><tr class="language"><th scope="row">Original language</th><td>English</td></tr><tr><th scope="row">Article number</th><td>635591</td></tr><tr><th scope="row">Journal</th><td><a rel="Journal" href="#" class="link"><span>Frontiers in Microbiology</span></a></td></tr><tr><th scope="row">Volume</th><td>12</td></tr><tr class="digital_object_identifiers"><th scope="row">DOIs</th><td><ul class="relations digital_object_identifiers"><li><a onclick="window.open(this.href, '_blank','noopener,noreferrer'); return false;" href="https://doi.org/10.3389/fmicb.2021.635591" class="link"><span>https://doi.org/10.3389/fmicb.2021.635591</span></a> </li></ul></td></tr><tr class="status"><th scope="row">Publication status</th><td><span class="prefix">Published - </span><span class="date">2021 Apr 27</span></td></tr></tbody></table></div></div> <div class="rendering rendering_researchoutput rendering_researchoutput_publicationbibliographicalnoteportalrenderer rendering_contributiontojournal rendering_publicationbibliographicalnoteportalrenderer rendering_contributiontojournal_publicationbibliographicalnoteportalrenderer"><h3 class="subheader">Bibliographical note</h3>Funding Information:<br />We acknowledge Karl Oldberg, Oonagh Shannon, and Gisela Hovold for important contribution with technical support and help. We also thank the Department of Clinical Microbiology, Office for Medical Services, Region Sk?ne, Lund, Sweden. Funding. This study was funded by the Royal Physiographic Society of Lund, the Thelma Zo?gas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Sk?ne University Hospital, ?sterlund and Lundgren.<br /><br />Funding Information:<br />This study was funded by the Royal Physiographic Society of Lund, the Thelma Zoégas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Skåne University Hospital, Österlund and Lundgren.<br /><br />Publisher Copyright:<br />© Copyright © 2021 Bläckberg, de Neergaard, Frick, Nordenfelt, Lood and Rasmussen.<br /><br />Copyright:<br />Copyright 2021 Elsevier B.V., All rights reserved.</div> <div class="rendering rendering_keywordable rendering_expertkeywordgrouspbytypeportal rendering_keywordable_expertkeywordgrouspbytypeportal"><div class="keyword-group"><h2 class="subheader">Subject classification (UKÄ)</h2><ul class="relations keywords"><li><span>Infectious Medicine</span></li></ul></div><div class="keyword-group"><h2 class="subheader">Free keywords</h2><ul class="relations keywords"><li class="userdefined-keyword"><span>antibody responses</span></li><li class="userdefined-keyword"><span>bacteraemia</span></li><li class="userdefined-keyword"><span>emm type</span></li><li class="userdefined-keyword"><span>recurrent infection</span></li><li class="userdefined-keyword"><span>Streptococcus dysgalactiae</span></li></ul></div></div> </div> <div class="content-sidebar publication-sidebar"> <h2 class="subheader">Access to Document</h2> <div class="rendering rendering_researchoutput rendering_researchoutput_publicationaccessrenderer rendering_contributiontojournal rendering_publicationaccessrenderer rendering_contributiontojournal_publicationaccessrenderer"><ul class="dois"><li class="open-access"><div class="doi"><a onclick="window.open(this.href, '_blank','noopener,noreferrer'); return false;" href="https://doi.org/10.3389/fmicb.2021.635591" class="link"><span>10.3389/fmicb.2021.635591</span></a><span class="meta">Licence: <a onclick="window.open(this.href, '_blank','noopener,noreferrer'); return false;" href="http://creativecommons.org/licenses/by/4.0/" class="link">CC BY</a></span></div></li></ul></div> </div> </div> </div> </section> <section class="page-section content-relation-section publication-fingerprint"> <div class="container"> <div class="header"> <h2 class="section-title"> <i class="icon icon-fingerprint" aria-hidden="true"></i> <span class="title">Fingerprint</span> </h2> <span class="sub-title"> Dive into the research topics of 'Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae'. 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aria-hidden="true" alt="" class="expand" /> <img src="/assets/gizmo-contract-7adbcd523b1972d72d537e479dd74798.svg" aria-hidden="true" alt="" class="contract" /> </span> <span class="inline-trend-graph"> <span class="year year-start">2022</span> <span class="trend-graph-wrapper"> <span class="bar" style="height: 100%;"> <span aria-expanded="false" role="tooltip">2022</span> </span> <span class="bar" style="height: 100%;"> <span aria-expanded="false" role="tooltip">2024</span> </span> </span> <span class="year year-end">2024</span> </span> </button> <ul class="content-statistics"> <li class=""> <a class="portal_link count increment-counter" aria-label=" 2 Doctoral Thesis (compilation)" href="/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fthesis%2Fdoccomp"><span> 2 </span></a> <span class="label">Doctoral Thesis (compilation)</span> </li> </ul> <script> $("ul.content-statistics li.index").last().addClass("separator"); </script> <div class="flex-break"></div> <div class="chart-container" id="chart-content-publication-by-year" style="display: none;" aria-hidden="true" tabindex="-1"> <p class="legend">Research output per year</p> <div class="chart-wrapper"> <canvas></canvas> </div> <p class="legend">Research output per year</p> </div> <table id="publication-container-table" class="sr-only" aria-hidden="true"></table> <script> $("#chart-content-publication-by-year").contentChart({ toggle: "button[rel='toggle-publication-chart']", title: "Research output per year", labels: [2022, 2023, 2024], values: [1, 0, 1], tableContainer: $('#publication-container-table'), }); </script> </div> </div> <div class="relation-list relation-list-publications"> <ul class="list-results"> <li class="list-result-item"> <div class="result-container"> <div class="rendering rendering_researchoutput rendering_researchoutput_portal-short rendering_thesis rendering_portal-short rendering_thesis_portal-short"><h3 class="title"><a rel="Thesis" href="https://portal.research.lu.se/en/publications/quantifying-phagocytosis-studies-on-the-antibody-response-during-" class="link"><span>Quantifying Phagocytosis – studies on the antibody response during invasive streptococcal infections</span></a></h3><a rel="Person" href="https://portal.research.lu.se/en/persons/therese-de-neergaard" class="link person"><span>de Neergaard, T.</span></a>, <span class="date">2024</span>, Lund: <a rel="Publisher" href="#" class="link"><span>Lund University, Faculty of Medicine</span></a>. <span class="numberofpages">113 p.</span><p class="type"><span class="type_family">Research output<span class="type_family_sep">: </span></span><span class="type_classification_parent">Thesis<span class="type_parent_sep"> › </span></span><span class="type_classification">Doctoral Thesis (compilation)</span></p></div> <div class="rendering rendering_researchoutput rendering_researchoutput_publicationattacheddocumentsportalrenderer rendering_thesis rendering_publicationattacheddocumentsportalrenderer rendering_thesis_publicationattacheddocumentsportalrenderer"><div class="open-access">Open Access</div><div class="files">File</div></div> <ul class="content-concept-list" role="menubar"> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="166c9cfe-19c9-4b77-bbba-285c03911ac5" data-rank-value="1.0" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Monospecific Antibody</span> <span class="value sr-only">100%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="0ef4c24c-c212-419d-b102-aa30bdb427c3" data-rank-value="0.7142857142857143" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Infectious Agent</span> <span class="value sr-only">71%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="430b05eb-3c99-4dea-98a9-95c6be9d5e42" data-rank-value="0.4285714285714286" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Immunity</span> <span class="value sr-only">42%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="5f1ea317-5787-4b52-84e9-db5e072fcf15" data-rank-value="0.28571428571428575" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Immune System</span> <span class="value sr-only">28%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="730ccbfa-43c3-462a-9d6a-d7f78f2e6313" data-rank-value="0.28571428571428575" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Innate Immune System</span> <span class="value sr-only">28%</span> </span> </button> <div class="dropdown-container"></div> </li> </ul> </div> </li> <li class="list-result-item"> <div class="result-container"> <div class="rendering rendering_researchoutput rendering_researchoutput_portal-short rendering_thesis rendering_portal-short rendering_thesis_portal-short"><h3 class="title"><a rel="Thesis" href="https://portal.research.lu.se/en/publications/interactions-between-%CE%B2-haemolytic-streptococci-and-the-human-host" class="link"><span>Interactions between β-haemolytic streptococci and the human host. Heart, skin and beyond.</span></a></h3><a rel="Person" href="https://portal.research.lu.se/en/persons/anna-bl%C3%A4ckberg" class="link person"><span>Bläckberg, A.</span></a>, <span class="date">2022</span>, Lund: <a rel="Publisher" href="#" class="link"><span>Lund University, Faculty of Medicine</span></a>. <span class="numberofpages">100 p.</span><p class="type"><span class="type_family">Research output<span class="type_family_sep">: </span></span><span class="type_classification_parent">Thesis<span class="type_parent_sep"> › </span></span><span class="type_classification">Doctoral Thesis (compilation)</span></p></div> <div class="rendering rendering_researchoutput rendering_researchoutput_publicationattacheddocumentsportalrenderer rendering_thesis rendering_publicationattacheddocumentsportalrenderer rendering_thesis_publicationattacheddocumentsportalrenderer"><div class="open-access">Open Access</div><div class="files">File</div></div> <ul class="content-concept-list" role="menubar"> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="8067db68-7952-4843-b28b-7830df949624" data-rank-value="1.0" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Skin</span> <span class="value sr-only">100%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="ea28b324-9f80-4c1a-825a-8704901732c8" data-rank-value="0.8" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Infective Endocarditis</span> <span class="value sr-only">80%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="3c420133-27cb-4cdf-acfe-515d00b5cded" data-rank-value="0.8" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Bacterial Endocarditis</span> <span class="value sr-only">80%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="a0e94a5b-42ba-424d-a45d-ba1b2dacf55d" data-rank-value="0.6" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Blood</span> <span class="value sr-only">60%</span> </span> </button> <div class="dropdown-container"></div> </li> <li class="concept-badge-small-container dropdown-overflow" role="none"> <button tabindex="-1" class="concept-badge-small tooltipped tooltipped-multiline tooltipped-e dropdown-toggle" role="menuitem" aria-expanded="false" aria-haspopup="true" data-concept-uuid="166c9cfe-19c9-4b77-bbba-285c03911ac5" data-rank-value="0.6" data-summary-url="/en/concepts/summary/"> <span class="concept-wrapper"> <span class="rank"></span> <span class="concept">Monospecific Antibody</span> <span class="value sr-only">60%</span> </span> </button> <div class="dropdown-container"></div> </li> </ul> </div> </li> </ul> </div> <div class="footer"> </div> </div> </section> <section class="page-section cite"> <div class="container"> <section class="publication-cite" aria-labelledby="publications-cite-title"> <h2 class="subheader" id="publications-cite-title">Cite this</h2> <ul class="tabs has-overflow-navigation" role="tablist" aria-label="Cite this"> <li class="selected" role="tab" id="tab-0" aria-controls="cite-apa" tabindex="-1" aria-selected="false" > APA </li> <li class="" role="tab" id="tab-1" aria-controls="cite-author" tabindex="-1" aria-selected="false" > Author </li> <li class="" role="tab" id="tab-2" aria-controls="cite-BIBTEX" tabindex="-1" aria-selected="false" > BIBTEX </li> <li class="" role="tab" id="tab-3" aria-controls="cite-harvard" tabindex="-1" aria-selected="false" > Harvard </li> <li class="" role="tab" id="tab-4" aria-controls="cite-standard" tabindex="-1" aria-selected="false" > Standard </li> <li class="" role="tab" id="tab-5" aria-controls="cite-RIS" tabindex="-1" aria-selected="false" > RIS </li> <li class="" role="tab" id="tab-6" aria-controls="cite-vancouver" tabindex="-1" aria-selected="false" > Vancouver </li> </ul> <div class="tab-container"> <div id="cite-apa" class="tab selected" role="tabpanel" aria-labelledby="tab-0" tabindex="-1" aria-hidden="true"> <div class="rendering rendering_researchoutput rendering_researchoutput_apa rendering_contributiontojournal rendering_apa rendering_contributiontojournal_apa"><a rel="Person" href="https://portal.research.lu.se/en/persons/anna-bl%C3%A4ckberg" class="link"><span>Bläckberg, A.</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/therese-de-neergaard" class="link"><span>, de Neergaard, T.</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/inga-maria-frick" class="link"><span>, Frick, I. M.</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/pontus-nordenfelt" class="link"><span>, Nordenfelt, P.</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/rolf-lood" class="link"><span>, Lood, R.</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/magnus-rasmussen" class="link"><span>, & Rasmussen, M.</span></a> (2021). <a rel="ContributionToJournal" href="https://portal.research.lu.se/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st" class="link"><span>Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae</span></a>. <a rel="Journal" href="#" class="link"><span><em>Frontiers in Microbiology</em></span></a>, <em>12</em>, Article 635591. <a onclick="window.open(this.href, '_blank','noopener,noreferrer'); return false;" href="https://doi.org/10.3389/fmicb.2021.635591" class="link"><span>https://doi.org/10.3389/fmicb.2021.635591</span></a></div> </div> <div id="cite-author" class="tab " role="tabpanel" aria-labelledby="tab-1" tabindex="-1" aria-hidden="true"> <div class="rendering rendering_researchoutput rendering_researchoutput_author rendering_contributiontojournal rendering_author rendering_contributiontojournal_author"><a rel="Person" href="https://portal.research.lu.se/en/persons/anna-bl%C3%A4ckberg" class="link"><span>Bläckberg, Anna</span></a> <a rel="Person" href="https://portal.research.lu.se/en/persons/therese-de-neergaard" class="link"><span> ; de Neergaard, Therese</span></a> <a rel="Person" href="https://portal.research.lu.se/en/persons/inga-maria-frick" class="link"><span> ; Frick, Inga Maria</span></a> et al. / <a rel="ContributionToJournal" href="https://portal.research.lu.se/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st" class="link"><span><strong>Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae</strong></span></a>. In: <a rel="Journal" href="#" class="link"><span>Frontiers in Microbiology</span></a>. 2021 ; Vol. 12.</div> </div> <div id="cite-BIBTEX" class="tab " role="tabpanel" aria-labelledby="tab-2" tabindex="-1" aria-hidden="true"> <div class="rendering rendering_researchoutput rendering_researchoutput_bibtex rendering_contributiontojournal rendering_bibtex rendering_contributiontojournal_bibtex"><div>@article{64e33a485aed4d499e747f8b42e029f3,</div><div> title = "Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae",</div><div> abstract = "Introduction: Streptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity. Materials and Methods: Patients with S. dysgalactiae bacteraemia in the county of Sk{\aa}ne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function. Results: Sixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent episodes of bacteraemia. Using ELISA with S. dysgalactiae isolates and mutants, development of IgG antibodies was demonstrated in few patients. Type-specific antibodies were demonstrated in one patient when recombinant M proteins as antigens, were applied. The type-specific serum mediated a small increase in phagocytosis but did not facilitate increased killing of the S. dysgalactiae isolate, carrying that M protein, in blood or by phagocytic cells. Conclusion: S. dysgalactiae bacteraemia sometimes results in increased levels of antibodies to the infecting pathogen. We did not find evidence that these antibodies are effectively opsonising. Apparent failure to produce opsonising antibodies might partially explain why S. dysgalactiae can cause recurrent invasive infections in the same host.",</div><div> keywords = "antibody responses, bacteraemia, emm type, recurrent infection, Streptococcus dysgalactiae",</div><div> author = "Anna Bl{\"a}ckberg and {de Neergaard}, Therese and Frick, {Inga Maria} and Pontus Nordenfelt and Rolf Lood and Magnus Rasmussen",</div><div> note = "Funding Information: We acknowledge Karl Oldberg, Oonagh Shannon, and Gisela Hovold for important contribution with technical support and help. We also thank the Department of Clinical Microbiology, Office for Medical Services, Region Sk?ne, Lund, Sweden. Funding. This study was funded by the Royal Physiographic Society of Lund, the Thelma Zo?gas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Sk?ne University Hospital, ?sterlund and Lundgren. Funding Information: This study was funded by the Royal Physiographic Society of Lund, the Thelma Zo{\'e}gas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Sk{\aa}ne University Hospital, {\"O}sterlund and Lundgren. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Bl{\"a}ckberg, de Neergaard, Frick, Nordenfelt, Lood and Rasmussen. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",</div><div> year = "2021",</div><div> month = apr,</div><div> day = "27",</div><div> doi = "10.3389/fmicb.2021.635591",</div><div> language = "English",</div><div> volume = "12",</div><div> journal = "Frontiers in Microbiology",</div><div> issn = "1664-302X",</div><div> publisher = "Frontiers Media S. A.",</div><p>}</p></div> </div> <div id="cite-harvard" class="tab " role="tabpanel" aria-labelledby="tab-3" tabindex="-1" aria-hidden="true"> <div class="rendering rendering_researchoutput rendering_researchoutput_harvard rendering_contributiontojournal rendering_harvard rendering_contributiontojournal_harvard"><a rel="Person" href="https://portal.research.lu.se/en/persons/anna-bl%C3%A4ckberg" class="link"><span>Bläckberg, A</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/therese-de-neergaard" class="link"><span>, de Neergaard, T</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/inga-maria-frick" class="link"><span>, Frick, IM</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/pontus-nordenfelt" class="link"><span>, Nordenfelt, P</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/rolf-lood" class="link"><span>, Lood, R</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/magnus-rasmussen" class="link"><span> & Rasmussen, M</span></a> 2021, '<a rel="ContributionToJournal" href="https://portal.research.lu.se/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st" class="link harvard_title"><span>Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae</span></a>', <a rel="Journal" href="#" class="link"><span><em>Frontiers in Microbiology</em></span></a>, vol. 12, 635591. <a onclick="window.open(this.href, '_blank','noopener,noreferrer'); return false;" href="https://doi.org/10.3389/fmicb.2021.635591" class="link"><span>https://doi.org/10.3389/fmicb.2021.635591</span></a></div> </div> <div id="cite-standard" class="tab " role="tabpanel" aria-labelledby="tab-4" tabindex="-1" aria-hidden="true"> <div class="rendering rendering_researchoutput rendering_researchoutput_standard rendering_contributiontojournal rendering_standard rendering_contributiontojournal_standard"><a rel="ContributionToJournal" href="https://portal.research.lu.se/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st" class="link"><span><strong>Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae.</strong></span></a> / <a rel="Person" href="https://portal.research.lu.se/en/persons/anna-bl%C3%A4ckberg" class="link"><span>Bläckberg, Anna</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/therese-de-neergaard" class="link"><span>; de Neergaard, Therese</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/inga-maria-frick" class="link"><span>; Frick, Inga Maria</span></a> et al. <br />In: <a rel="Journal" href="#" class="link"><span>Frontiers in Microbiology</span></a>, Vol. 12, 635591, 27.04.2021.<p class="type"><span class="type_family">Research output<span class="type_family_sep">: </span></span><span class="type_classification_parent">Contribution to journal<span class="type_parent_sep"> › </span></span><span class="type_classification">Article</span><span class="type_classification"><span class="type_parent_sep"> › </span>peer-review</span></p></div> </div> <div id="cite-RIS" class="tab " role="tabpanel" aria-labelledby="tab-5" tabindex="-1" aria-hidden="true"> <div class="rendering rendering_researchoutput rendering_researchoutput_ris rendering_contributiontojournal rendering_ris rendering_contributiontojournal_ris"><p>TY - JOUR</p><p>T1 - Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae</p><p>AU - Bläckberg, Anna</p><p>AU - de Neergaard, Therese</p><p>AU - Frick, Inga Maria</p><p>AU - Nordenfelt, Pontus</p><p>AU - Lood, Rolf</p><p>AU - Rasmussen, Magnus</p><p>N1 - Funding Information: We acknowledge Karl Oldberg, Oonagh Shannon, and Gisela Hovold for important contribution with technical support and help. We also thank the Department of Clinical Microbiology, Office for Medical Services, Region Sk?ne, Lund, Sweden. Funding. This study was funded by the Royal Physiographic Society of Lund, the Thelma Zo?gas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Sk?ne University Hospital, ?sterlund and Lundgren. Funding Information: This study was funded by the Royal Physiographic Society of Lund, the Thelma Zoégas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Skåne University Hospital, Österlund and Lundgren. Publisher Copyright: © Copyright © 2021 Bläckberg, de Neergaard, Frick, Nordenfelt, Lood and Rasmussen. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.</p><p>PY - 2021/4/27</p><p>Y1 - 2021/4/27</p><p>N2 - Introduction: Streptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity. Materials and Methods: Patients with S. dysgalactiae bacteraemia in the county of Skåne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function. Results: Sixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent episodes of bacteraemia. Using ELISA with S. dysgalactiae isolates and mutants, development of IgG antibodies was demonstrated in few patients. Type-specific antibodies were demonstrated in one patient when recombinant M proteins as antigens, were applied. The type-specific serum mediated a small increase in phagocytosis but did not facilitate increased killing of the S. dysgalactiae isolate, carrying that M protein, in blood or by phagocytic cells. Conclusion: S. dysgalactiae bacteraemia sometimes results in increased levels of antibodies to the infecting pathogen. We did not find evidence that these antibodies are effectively opsonising. Apparent failure to produce opsonising antibodies might partially explain why S. dysgalactiae can cause recurrent invasive infections in the same host.</p><p>AB - Introduction: Streptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity. Materials and Methods: Patients with S. dysgalactiae bacteraemia in the county of Skåne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function. Results: Sixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent episodes of bacteraemia. Using ELISA with S. dysgalactiae isolates and mutants, development of IgG antibodies was demonstrated in few patients. Type-specific antibodies were demonstrated in one patient when recombinant M proteins as antigens, were applied. The type-specific serum mediated a small increase in phagocytosis but did not facilitate increased killing of the S. dysgalactiae isolate, carrying that M protein, in blood or by phagocytic cells. Conclusion: S. dysgalactiae bacteraemia sometimes results in increased levels of antibodies to the infecting pathogen. We did not find evidence that these antibodies are effectively opsonising. Apparent failure to produce opsonising antibodies might partially explain why S. dysgalactiae can cause recurrent invasive infections in the same host.</p><p>KW - antibody responses</p><p>KW - bacteraemia</p><p>KW - emm type</p><p>KW - recurrent infection</p><p>KW - Streptococcus dysgalactiae</p><p>U2 - 10.3389/fmicb.2021.635591</p><p>DO - 10.3389/fmicb.2021.635591</p><p>M3 - Article</p><p>C2 - 33986732</p><p>AN - SCOPUS:85105635824</p><p>SN - 1664-302X</p><p>VL - 12</p><p>JO - Frontiers in Microbiology</p><p>JF - Frontiers in Microbiology</p><p>M1 - 635591</p><p>ER - </p></div> </div> <div id="cite-vancouver" class="tab " role="tabpanel" aria-labelledby="tab-6" tabindex="-1" aria-hidden="true"> <div class="rendering rendering_researchoutput rendering_researchoutput_vancouver rendering_contributiontojournal rendering_vancouver rendering_contributiontojournal_vancouver"><a rel="Person" href="https://portal.research.lu.se/en/persons/anna-bl%C3%A4ckberg" class="link"><span>Bläckberg A</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/therese-de-neergaard" class="link"><span>, de Neergaard T</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/inga-maria-frick" class="link"><span>, Frick IM</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/pontus-nordenfelt" class="link"><span>, Nordenfelt P</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/rolf-lood" class="link"><span>, Lood R</span></a><a rel="Person" href="https://portal.research.lu.se/en/persons/magnus-rasmussen" class="link"><span>, Rasmussen M</span></a>. <span class="title"><a rel="ContributionToJournal" href="https://portal.research.lu.se/en/publications/lack-of-opsonic-antibody-responses-to-invasive-infections-with-st" class="link"><span>Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae</span></a></span>. <a rel="Journal" href="#" class="link"><span>Frontiers in Microbiology</span></a>. 2021 Apr 27;12:635591. doi: 10.3389/fmicb.2021.635591</div> </div> <script> $(function() { $(".tab-container .rendering a:not([href^='http://dx.doi.org'])").contents().unwrap(); 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Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae - Lund University