beta Tubulin Antibody - BSA Free (NB600-936) by Novus, Part of Bio-Techne

<!DOCTYPE html> <html lang="en" dir="ltr" prefix="og: https://ogp.me/ns#"> <head> <meta charset="utf-8" /> <noscript><style>form.antibot * :not(.antibot-message) { display: none !important; }</style> </noscript><meta name="applicable-device" content="pc,mobile" /> <link rel="canonical" href="https://www.bio-techne.com/p/antibodies/beta-tubulin-antibody_nb600-936" /> <meta property="og:site_name" content="Bio-Techne" /> <meta property="og:type" content="Website" /> <meta property="og:url" content="https://www.bio-techne.com/" /> <meta property="og:title" content="beta Tubulin Antibody - BSA Free (NB600-936) by Novus, Part of Bio-Techne" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <script type="application/ld+json">{ "@context": "https://schema.org", "@graph": [ { "@type": "WebPage", "@id": "https://www.bio-techne.com/p/antibodies/beta-tubulin-antibody_nb600-936", "breadcrumb": { "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Home", "item": "https://www.bio-techne.com/" }, { "@type": "ListItem", "position": 2, "name": "beta Tubulin", "item": "https://www.bio-techne.com/t/beta-tubulin" }, { "@type": "ListItem", "position": 3, "name": "beta Tubulin Antibodies", "item": "https://www.bio-techne.com/t/beta-tubulin/antibodies" } ] } } ] }</script> <meta http-equiv="x-dns-prefetch-control" content="on" /> <meta http-equiv="x-ua-compatible" content="ie=edge" /> <meta name="keywords" content="" /> <meta name="description" content="Cited in 94 publications. View beta Tubulin Antibody - BSA Free (NB600-936) validated in 13 species & 5 applications from Novus Biologicals." /> <script type="application/ld+json" id="json-ld">[{"@context":"http://schema.org","@type":"Product","additionalProperty":[{"additionalProperty":{"@type":"PropertyValue","name":"Applications","value":"Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot"}},{"additionalProperty":{"@type":"PropertyValue","name":"Immunogen","value":"A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). 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Preferably not from a mouse host. Can you help?\r\n","acceptedAnswer":{"@type":"Answer","text":"According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459\r\n"}}]}]</script> <link rel="alternate" hreflang="en" href="https://www.bio-techne.com/p/antibodies/beta-tubulin-antibody_nb600-936" /> <link rel="alternate" hreflang="zh-hans" href="https://www.bio-techne.com/cn/p/antibodies/beta-tubulin-antibody_nb600-936" /> <link rel="icon" href="/themes/custom/bio_techne_global/favicon.ico" type="image/vnd.microsoft.icon" /> <link rel="preconnect" href="https://resources.bio-techne.com" /> <link rel="preconnect" href="https://resources.tocris.com" /> <link rel="preconnect" href="https://i.icomoon.io" /> <link rel="preconnect" href="https://cdn.icomoon.io" /> <link rel="dns-prefetch" href="https://connect.facebook.net" /> <link rel="dns-prefetch" href="https://resources.bio-techne.com" /> <link rel="dns-prefetch" href="https://snap.licdn.com" /> <link rel="dns-prefetch" href="https://www.google-analytics.com" /> <link rel="dns-prefetch" 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id="block-bio-techne-global-content" class="system_main_block"> <div id="product-page-details" data-template_in_use="antibody_template" data-time_total="0.65771102905273" data-time_to_compile="0.11641001701355" data-time_to_build="0.73462009429932" data-time_datapoints="-0.19331908226013" data-time_template="0.19331908226013" data-time_post_epcc="0.85103011131287" data-time_epcc="-0.19331908226013" data-render_time="Monday 17th of February 2025 07:07:20 AM" data-fallback="FALSE" data-time_to_process_data="0.11772608757019" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="validation-badges-header-wrapper"> <a class="biological-validation mr-3 d-inline-flex mt-1" href="/reagents/antibodies/antibody-validation" target="_blank" title="Biological Validation"></a> </div> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> beta Tubulin Antibody - BSA Free </h1> <h2 class="font-weight-normal h4"> Novus Biologicals, part of Bio-Techne | Catalog # <span id="product-code" class="font-weight-bold">NB600-936</span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="reviews_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="reviews_quicklink"> <a href="#reviews" id="review_count_quicklink" class="reviews" data-tab="2"> <span class="review_stars review_stars_4"></span> <span class="font-weight-bold"></span> (7) </a> </div> </div> <div id="citation_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="citation_quicklink"> <a href="#citations" id="citation_count" data-tab="2" class="citations"> <span class="icon-icon-product-citations d-none d-lg-inline pr-1"></span> Citations (94)  </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <div class="px-0 my-1" id="datasheet-summary-wrapper" data-section="section-base--top-jumplink"> <article class="top-jumplink-wrapper"> <span class="icon-icon-pdf text-error-red"></span> <a href="#product-documents" class="product-documents-top-link" rel="nofollow">Product Datasheet / COA / SDS / Protocols & Manuals</a> </article> </div> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" alt="Novus Biologicals, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> <div class="row border-top mb-2" id="images-orderdetail-wrapper"> <div class="col-xl-6 mt-2" id="images-wrapper" data-section="image_summary"> <div id="image-tree" data-section="component-product-image-tree" class="vertical lightbox" data-mdb-zoom-effect="true"> <div id="product-image-gallery" class="row"> <div class="col-md-2 d-none d-md-block"> <ul class="list-inline m-0"> <li data-id="1" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Simple-Western-NB600-936-img0016.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Simple-Western-NB600-936-img0016.jpg" alt="Simple Western: beta Tubulin AntibodyBSA Free [NB600-936]" title="Simple Western: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Simple-Western-NB600-936-img0016.jpg" data-id="0" data-application="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Simple-Western-NB600-936-img0016.jpg" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="lazy" /> </div> </li> <li data-id="2" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0006.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0006.jpg" alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000" data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0006.jpg" data-id="1" data-application="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0006.jpg" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="lazy" /> </div> </li> <li data-id="3" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg" alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in C2C12 cells, at 5% and 18% O2. (b, c) Average Nox1 signal How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30363917/) licensed under a CC-BY license." data-badges='<span class="biological-validation"></span>' data-mdb-img="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg" data-id="2" data-application="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="lazy" /> </div> </li> <li data-id="4" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0023.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="5" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0011.jpg" data-alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="6" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0004.jpg" data-alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="7" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0024.jpg" data-alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep29311), licensed under a CC-BY license." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="8" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0015.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue)." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="9" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0001.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="10" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0022.jpg" data-alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="11" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0014.jpg" data-alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="12" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0013.jpg" data-alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="13" class="none"> <i data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0012.jpg" data-alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="14" class="none"> <i data-src="https://resources.bio-techne.com/images/products/antibody/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-immunocytochemistry-immunofluorescence-2062024155114..jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Beta Tubulin in A431 Human Cell Line." data-caption="Beta Tubulin was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-936AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="15" class="none"> <i data-src="https://resources.bio-techne.com/images/products/antibody/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-immunocytochemistry-immunofluorescence-206202415534..jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Beta Tubulin in U-2 OS Human Cell Line." data-caption="Beta Tubulin was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB600-936G) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="16" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415175297.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Dual pathway inhibition regulates TK1 protein levels & results in greater p27 protein levels than single agents alone.Single agent PLX4032 resulted in activation of p-AKT Ser473 following 24 hours of exposure at two concentrations (100 nM, 1 µM). The addition of the dual PI3K/mTOR inhibitor BEZ235 blocks p-AKT Ser473 activation & resulted in a greater increase in p27 protein levels & diminished TK1 protein levels. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="17" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415334929.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of pluripotency related markers & a sphere culture of BEAS-2B under normoxia.(a) HIF-2 alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin & TATA binding protein (TBP) were used as protein markers for the cytosol & nucleus fraction, respectively. (b) Western blotting for Oct-4. (c) For knockdown of HIF-2 alpha, BEAS-2B cells were transfected with shRNA targeting HIF-2 alpha & sh-Luc was used as control. (d) Western blotting of Nanog & a culture of BEAS-2B containing floating spheres. Representative result from two or three experiments was presented. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27373565), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="18" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415384143.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of AAV transgene before & after experimental autoimmune uveoretinitis (EAU) induction. (A) B10.RIII mice were evaluated by funduscopy one month after an intravitreal injection of AAV delivering sGFP-TatM013v5 or secreted GFP (sGFP). As a control we evaluated mice that received an intravitreal injection of saline. Diffuse fluorescence indicates the secretion of sGFP & sGFP-TatM013v5 in the retina. (B) Western blot from retinas harvested 14 days after IRBP immunization. Membrane was probed with anti-GFP & anti-Tubulin antibodies. Image shows expression of sGFP on both sGFP & sGFP-TatM013v5 retina lysates. (n = 2–3 retina samples from different mice per group). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31795515), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="19" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416171469.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Combined V600EBRAF & mTOR inhibition results in transcriptional control of TK1 protein levels in COLO 205 cells.(A) Western blot of COLO 205 cells treated with PP242 (250 nM) & increasing PLX4032. Similar to single agent PLX4032, p-MEK, but not p-ERK, was inhibited in a PLX4032-dependent manner. Consistent with mTORC1/mTORC2 inhibition, p-AKT Ser473, but not p-AKT Thr308, was inhibited. Unlike single agent PLX4032, which resulted in concentration-dependent activation of p-rpS6, combined treatment maintained p-rpS6 levels at essentially baseline levels except at the highest PLX4032 concentration. Similarly, DUSP6 levels were inversely related to p-ERK protein levels. With combined mTOR & V600EBRAF blockade, p27 & TK1 protein levels were inversely correlated & dramatically affected by PLX4032 exposure. (B) Similarly, TK1 mRNA was significantly reduced at PLX4032 concentrations as low as 10 nM. (C) Despite elevated p27 protein levels, p27 mRNA was unaffected by combined mTOR-V600EBRAF inhibition. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="20" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241617144.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - [18F]-FLT PET reflects BEZ235-dependent inhibition of PI3K/mTOR activity in PLX4720 treated COLO 205 xenografts. Xenograft-bearing mice were imaged with [18F]-FLT PET on treatment day 4. (A) [18F]-FLT uptake was diminished in the combination treatment cohort relative to vehicle (p = 0.0087), but not single agent PLX4720- or BEZ235-treated cohorts. (B) Western blot of xenograft tissue harvested immediately following imaging illustrated elevated p-ERK & p-rpS6 levels in PLX4720-treated mice. Combining PLX4032 with BEZ235 resulted in reduced p-ERK & p-rpS6 protein levels. (C) TK1 levels, as measured by IHC, were reduced only in the combination treatment group in agreement with [18F]-FLT PET. (D) Consistent with in vitro studies, diminished TK1 levels, & consequently [18F]-FLT PET, correlated with elevated p27 that was elevated only in the combination treated group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="21" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416171490.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - PLX4720 exposure does not affect [18F]-FLT PET in COLO 205 xenografts, despite evidence of target inhibition & diminished [18F]-FDG uptake.(A) Representative transverse [18F]-FLT & [18F]-FDG PET images acquired after three daily treatments with vehicle or 60 mg/kg PLX4720 (tumor indicated by arrowhead). (B) Quantification of PET data illustrated similar [18F]-FLT uptake in vehicle-treated & PLX4720-treated tumors. Unlike [18F]-FLT PET, PLX4720 exposure elicited a significant reduction in [18F]-FDG uptake (p = 0.0006). (C) Western blot analysis of vehicle- & PLX4720-treated tumor tissue confirmed that PLX4720 had no effect on TK1 protein levels in agreement with [18F]-FLT PET. Target inhibition as measured by p-MEK levels was observed. However, similar to in vitro studies, PLX4720-treated COLO 205 xenografts exhibited elevated p-ERK & p-rpS6 protein levels relative to vehicle controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="22" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241616030.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="23" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241616031.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li data-id="24" class="none"> <i data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416175297.jpg" data-alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - TK1 protein levels do not reflect p-ERK attenuation following inhibition of V600EBRAF inhibition in COLO 205 cells.COLO 205 cells were collected 48 hours of PLX 4032 exposure at 10 nM, 100 nM, 500 nM, 1 µM, or 5 µM. (A) Western blot analysis demonstrated target inhibition of p-MEK despite increased p-ERK levels. PI3K-mTOR signaling was elevated in a PLX 4032-dependent manner as exhibited by a steady rise in p-rpS6 levels. The ERK-phosphatase DUSP6 decreased in conjunction with mTOR signaling & was inversely proportional to p-ERK levels. A slight increase in p27 levels were observed concomitantly with only modest changes in TK1 levels, except at the highest dose of PLX4032. (B) Decreased TK1 mRNA levels were observed at all drug concentrations above 10 nM (p<0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" class="none"></i> </li> <li class="list-inline-item"> <div class="image-slider-container"> <div class="lightbox product-image-lightbox"> <div class="lightbox border text-bt-dark-blue text-center search_result_thumbnail_image bg-light-gray rounded" style="padding: 20px 10px" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification]." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0011.jpg" data-id="3" data-application="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0011.jpg" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936"> +21 <br>images </div> </div> </div> </li> </ul> </div> <div class="col-md-10 border rounded d-flex align-items-center justify-content-center product-image-lightbox"> <div class="text-center my-2" data-brand="novus"> <img src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0021.jpg" alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0021.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0021.jpg" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges='' data-id="0" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="NB600-936"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="NB600-936SS" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">NB600-936SS</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="NB600-936" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">NB600-936</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="promo-content alert alert-success" role="alert"> <p>Save on RUO reagents! 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data-titleoverwrite="Alexa Fluor 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936af350">Alexa Fluor 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936af350">NB600-936AF350</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beta-tubulin-antibody_nb600-936af350" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB600-936AF405" data-titleoverwrite="Alexa Fluor 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936af405">Alexa Fluor 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936af405">NB600-936AF405</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beta-tubulin-antibody_nb600-936af405" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB600-936AF488" data-titleoverwrite="Alexa Fluor 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936af488">Alexa Fluor 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936af488">NB600-936AF488</a> </div> </div> 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data-titleoverwrite="mFluor Violet 450 SE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936mfv450">mFluor Violet 450 SE</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/beta-tubulin-antibody_nb600-936mfv450">NB600-936MFV450</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/beta-tubulin-antibody_nb600-936mfv450" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="NB600-936MFV500" data-titleoverwrite="mFluor Violet 500 SE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a 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by</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Biological Validation </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Species Reactivity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> Human, Mouse, Rat, Porcine, Bovine, C. elegans, Chicken, Chinese Hamster, Insect, Invertebrate, Primate, Xenopus, Zebrafish </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> Human, Mouse, Rat, Porcine, Bovine, Frog - Xenopus (African Clawed Frog), Invertebrate, Nematode - Caenorhabditis elegans, Primate </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Applications</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <h4 class="d-block">Validated:</h4> <div class="d-block pb-2"> Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot </div> <h4 class="d-block">Cited:</h4> <div class="d-block pb-2"> Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Label</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Unconjugated </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Antibody Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Polyclonal Rabbit IgG </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Format</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> BSA Free </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Concentration</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 1.0 mg/ml </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/beta-tubulin/antibodies">View all beta Tubulin Antibodies »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="NB600-936" data-type=viewed></div> <div id="product-specifications" class="bt-pp-border-bottom py-5" data-section="section-base--product-specifications"> <h3>Product Specifications</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Immunogen</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Reactivity Notes</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Use in Rat reported in scientific literature (PMID:34519641). Invertebrate reactivity reported in scientific literature (PMID: 28114363). C. elegans reactivity reported in scientific literature (PMID: 27690361). Insect reactivity reported from a verified customer review. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Localization</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Major constituent of microtubules </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Marker</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Microtubule Marker </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Clonality</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Polyclonal </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Host</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Rabbit </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Isotype</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> IgG </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Theoretical MW</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 50 kDa.<br>Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. </div> </div> </div> </div> <div id="scientific-data" class="bt-pp-border-bottom py-5" data-anchor-label="Scientific Data" data-section="section-base--scientific-data"> <div> <h3>Scientific Data Images for beta Tubulin Antibody - BSA Free </h3> </div> <div class="product-data-wrapper mt-5" id="scientific-data-table" data-scientific-count="22"> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0021.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0021.jpg" alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. </div> </div> </div> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Simple-Western-NB600-936-img0016.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Simple-Western-NB600-936-img0016.jpg" alt="Simple Western: beta Tubulin AntibodyBSA Free [NB600-936]" title="Simple Western: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." data-badges="" data-application="Simple Western" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Simple Western: beta Tubulin AntibodyBSA Free [NB600-936]</h4> Simple Western: beta Tubulin Antibody [NB600-936] - Lane view shows a specific band for beta Tubulin in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. </div> </div> </div> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0006.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0006.jpg" alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000" data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]</h4> Western Blot: beta Tubulin Antibody [NB600-936] - Analysis of extracts from HeLa, A549 and Jurkat cells using NB600-936 Beta-tubulin antibody at 1:1000 </div> </div> </div> <span class="product-data-wrapper table" id="more-scientific-data" style="display:none"> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0020.jpg" alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in C2C12 cells, at 5% and 18% O2. (b, c) Average Nox1 signal How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30363917/) licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <div class="validation-badges-header-wrapper"> <a class="biological-validation mr-3 d-inline-flex mt-1" href="/reagents/antibodies/antibody-validation" target="_blank" title="Biological Validation"></a> </div> <h4 class="font-weight-bold">Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - beta Tubulin Antibody [NB600-936] - Reduced levels of NADPH oxidases 1 and 4 at 18% versus 5% O2. (a) Representative Western blots showing Nox1 and Beta-tubulin in PC-3 cells or Nox4 and Beta-tubulin in C2C12 cells, at 5% and 18% O2. (b, c) Average Nox1 signal How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30363917/) licensed under a CC-BY license. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0023.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0023.jpg" alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0011.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0011.jpg" alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human skin using 1:2000 dilution of beta Tubulin antibody. Intense cytoplasmic staining of beta Tubulin (TUBB) protein was observed in various cells of the epidermal as well as the dermal cells [10X Magnification]. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0004.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0004.jpg" alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]</h4> Western Blot: beta Tubulin Antibody [NB600-936] - Analysis in HeLa whole cell lysate at a 1:1,000 dilution. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0024.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Western-Blot-NB600-936-img0024.jpg" alt="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" title="Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]" data-caption="Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep29311), licensed under a CC-BY license." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin AntibodyBSA Free [NB600-936]</h4> Western Blot: beta Tubulin Antibody [NB600-936] - Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia. HIF-2alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep29311), licensed under a CC-BY license. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0015.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0015.jpg" alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue)." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Confocal immunofluorescent analysis of C2C12 cells using beta Tubulin antibody (NB600-936, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0001.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0001.jpg" alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - Analysis of beta Tubulin in mouse hippocampal primary culture. Image courtesy of product review by Lin Yi-Wen. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0022.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-936-img0022.jpg" alt="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NB600-936] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-beta Tubulin Antibody (NB600-936) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0014.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0014.jpg" alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. Strong cytoplasmic immuno-positivity of beta Tubulin (TUBB) was observed in SCC cells as well as the associated tumor stromal cells [10X Magnification]. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0013.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0013.jpg" alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using 1:2000 dilution of beta Tubulin antibody. This representative image shows a cytoplasm specific staining of beta Tubulin (TUBB) in SCC cells [60X Magnification]. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0012.jpg" data-srcset="https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-Immunohistochemistry-Paraffin-NB600-936-img0012.jpg" alt="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" title="Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]" data-caption="Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification]." data-badges="" data-application="Immunohistochemistry-Paraffin" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Immunohistochemistry-Paraffin: beta Tubulin Antibody - BSA Free [NB600-936]</h4> Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB600-936] - Analysis of FFPE tissue section of normal human brain using 1:2000 dilution of beta Tubulin antibody. The various brain cells depicted strong cytoplasmic immunoreactivity of beta Tubulin (TUBB) protein [10X Magnification]. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-immunocytochemistry-immunofluorescence-2062024155114..jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-immunocytochemistry-immunofluorescence-2062024155114..jpg" alt="beta Tubulin Antibody - BSA Free" title="Beta Tubulin in A431 Human Cell Line." data-caption="Beta Tubulin was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-936AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Beta Tubulin in A431 Human Cell Line.</h4> Beta Tubulin was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB600-936AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-immunocytochemistry-immunofluorescence-206202415534..jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-immunocytochemistry-immunofluorescence-206202415534..jpg" alt="beta Tubulin Antibody - BSA Free" title="Beta Tubulin in U-2 OS Human Cell Line." data-caption="Beta Tubulin was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB600-936G) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Beta Tubulin in U-2 OS Human Cell Line.</h4> Beta Tubulin was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-beta Tubulin Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB600-936G) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415175297.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415175297.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Dual pathway inhibition regulates TK1 protein levels & results in greater p27 protein levels than single agents alone.Single agent PLX4032 resulted in activation of p-AKT Ser473 following 24 hours of exposure at two concentrations (100 nM, 1 µM). The addition of the dual PI3K/mTOR inhibitor BEZ235 blocks p-AKT Ser473 activation & resulted in a greater increase in p27 protein levels & diminished TK1 protein levels. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Dual pathway inhibition regulates TK1 protein levels & results in greater p27 protein levels than single agents alone.Single agent PLX4032 resulted in activation of p-AKT Ser473 following 24 hours of exposure at two concentrations (100 nM, 1 µM). The addition of the dual PI3K/mTOR inhibitor BEZ235 blocks p-AKT Ser473 activation & resulted in a greater increase in p27 protein levels & diminished TK1 protein levels. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415334929.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415334929.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of pluripotency related markers & a sphere culture of BEAS-2B under normoxia.(a) HIF-2 alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin & TATA binding protein (TBP) were used as protein markers for the cytosol & nucleus fraction, respectively. (b) Western blotting for Oct-4. (c) For knockdown of HIF-2 alpha, BEAS-2B cells were transfected with shRNA targeting HIF-2 alpha & sh-Luc was used as control. (d) Western blotting of Nanog & a culture of BEAS-2B containing floating spheres. Representative result from two or three experiments was presented. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27373565), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of pluripotency related markers & a sphere culture of BEAS-2B under normoxia.(a) HIF-2 alpha expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin & TATA binding protein (TBP) were used as protein markers for the cytosol & nucleus fraction, respectively. (b) Western blotting for Oct-4. (c) For knockdown of HIF-2 alpha, BEAS-2B cells were transfected with shRNA targeting HIF-2 alpha & sh-Luc was used as control. (d) Western blotting of Nanog & a culture of BEAS-2B containing floating spheres. Representative result from two or three experiments was presented. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27373565), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415384143.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202415384143.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of AAV transgene before & after experimental autoimmune uveoretinitis (EAU) induction. (A) B10.RIII mice were evaluated by funduscopy one month after an intravitreal injection of AAV delivering sGFP-TatM013v5 or secreted GFP (sGFP). As a control we evaluated mice that received an intravitreal injection of saline. Diffuse fluorescence indicates the secretion of sGFP & sGFP-TatM013v5 in the retina. (B) Western blot from retinas harvested 14 days after IRBP immunization. Membrane was probed with anti-GFP & anti-Tubulin antibodies. Image shows expression of sGFP on both sGFP & sGFP-TatM013v5 retina lysates. (n = 2–3 retina samples from different mice per group). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31795515), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Expression of AAV transgene before & after experimental autoimmune uveoretinitis (EAU) induction. (A) B10.RIII mice were evaluated by funduscopy one month after an intravitreal injection of AAV delivering sGFP-TatM013v5 or secreted GFP (sGFP). As a control we evaluated mice that received an intravitreal injection of saline. Diffuse fluorescence indicates the secretion of sGFP & sGFP-TatM013v5 in the retina. (B) Western blot from retinas harvested 14 days after IRBP immunization. Membrane was probed with anti-GFP & anti-Tubulin antibodies. Image shows expression of sGFP on both sGFP & sGFP-TatM013v5 retina lysates. (n = 2–3 retina samples from different mice per group). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31795515), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416171469.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416171469.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Combined V600EBRAF & mTOR inhibition results in transcriptional control of TK1 protein levels in COLO 205 cells.(A) Western blot of COLO 205 cells treated with PP242 (250 nM) & increasing PLX4032. Similar to single agent PLX4032, p-MEK, but not p-ERK, was inhibited in a PLX4032-dependent manner. Consistent with mTORC1/mTORC2 inhibition, p-AKT Ser473, but not p-AKT Thr308, was inhibited. Unlike single agent PLX4032, which resulted in concentration-dependent activation of p-rpS6, combined treatment maintained p-rpS6 levels at essentially baseline levels except at the highest PLX4032 concentration. Similarly, DUSP6 levels were inversely related to p-ERK protein levels. With combined mTOR & V600EBRAF blockade, p27 & TK1 protein levels were inversely correlated & dramatically affected by PLX4032 exposure. (B) Similarly, TK1 mRNA was significantly reduced at PLX4032 concentrations as low as 10 nM. (C) Despite elevated p27 protein levels, p27 mRNA was unaffected by combined mTOR-V600EBRAF inhibition. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Combined V600EBRAF & mTOR inhibition results in transcriptional control of TK1 protein levels in COLO 205 cells.(A) Western blot of COLO 205 cells treated with PP242 (250 nM) & increasing PLX4032. Similar to single agent PLX4032, p-MEK, but not p-ERK, was inhibited in a PLX4032-dependent manner. Consistent with mTORC1/mTORC2 inhibition, p-AKT Ser473, but not p-AKT Thr308, was inhibited. Unlike single agent PLX4032, which resulted in concentration-dependent activation of p-rpS6, combined treatment maintained p-rpS6 levels at essentially baseline levels except at the highest PLX4032 concentration. Similarly, DUSP6 levels were inversely related to p-ERK protein levels. With combined mTOR & V600EBRAF blockade, p27 & TK1 protein levels were inversely correlated & dramatically affected by PLX4032 exposure. (B) Similarly, TK1 mRNA was significantly reduced at PLX4032 concentrations as low as 10 nM. (C) Despite elevated p27 protein levels, p27 mRNA was unaffected by combined mTOR-V600EBRAF inhibition. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241617144.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241617144.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - [18F]-FLT PET reflects BEZ235-dependent inhibition of PI3K/mTOR activity in PLX4720 treated COLO 205 xenografts. Xenograft-bearing mice were imaged with [18F]-FLT PET on treatment day 4. (A) [18F]-FLT uptake was diminished in the combination treatment cohort relative to vehicle (p = 0.0087), but not single agent PLX4720- or BEZ235-treated cohorts. (B) Western blot of xenograft tissue harvested immediately following imaging illustrated elevated p-ERK & p-rpS6 levels in PLX4720-treated mice. Combining PLX4032 with BEZ235 resulted in reduced p-ERK & p-rpS6 protein levels. (C) TK1 levels, as measured by IHC, were reduced only in the combination treatment group in agreement with [18F]-FLT PET. (D) Consistent with in vitro studies, diminished TK1 levels, & consequently [18F]-FLT PET, correlated with elevated p27 that was elevated only in the combination treated group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - [18F]-FLT PET reflects BEZ235-dependent inhibition of PI3K/mTOR activity in PLX4720 treated COLO 205 xenografts. Xenograft-bearing mice were imaged with [18F]-FLT PET on treatment day 4. (A) [18F]-FLT uptake was diminished in the combination treatment cohort relative to vehicle (p = 0.0087), but not single agent PLX4720- or BEZ235-treated cohorts. (B) Western blot of xenograft tissue harvested immediately following imaging illustrated elevated p-ERK & p-rpS6 levels in PLX4720-treated mice. Combining PLX4032 with BEZ235 resulted in reduced p-ERK & p-rpS6 protein levels. (C) TK1 levels, as measured by IHC, were reduced only in the combination treatment group in agreement with [18F]-FLT PET. (D) Consistent with in vitro studies, diminished TK1 levels, & consequently [18F]-FLT PET, correlated with elevated p27 that was elevated only in the combination treated group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416171490.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416171490.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - PLX4720 exposure does not affect [18F]-FLT PET in COLO 205 xenografts, despite evidence of target inhibition & diminished [18F]-FDG uptake.(A) Representative transverse [18F]-FLT & [18F]-FDG PET images acquired after three daily treatments with vehicle or 60 mg/kg PLX4720 (tumor indicated by arrowhead). (B) Quantification of PET data illustrated similar [18F]-FLT uptake in vehicle-treated & PLX4720-treated tumors. Unlike [18F]-FLT PET, PLX4720 exposure elicited a significant reduction in [18F]-FDG uptake (p = 0.0006). (C) Western blot analysis of vehicle- & PLX4720-treated tumor tissue confirmed that PLX4720 had no effect on TK1 protein levels in agreement with [18F]-FLT PET. Target inhibition as measured by p-MEK levels was observed. However, similar to in vitro studies, PLX4720-treated COLO 205 xenografts exhibited elevated p-ERK & p-rpS6 protein levels relative to vehicle controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - PLX4720 exposure does not affect [18F]-FLT PET in COLO 205 xenografts, despite evidence of target inhibition & diminished [18F]-FDG uptake.(A) Representative transverse [18F]-FLT & [18F]-FDG PET images acquired after three daily treatments with vehicle or 60 mg/kg PLX4720 (tumor indicated by arrowhead). (B) Quantification of PET data illustrated similar [18F]-FLT uptake in vehicle-treated & PLX4720-treated tumors. Unlike [18F]-FLT PET, PLX4720 exposure elicited a significant reduction in [18F]-FDG uptake (p = 0.0006). (C) Western blot analysis of vehicle- & PLX4720-treated tumor tissue confirmed that PLX4720 had no effect on TK1 protein levels in agreement with [18F]-FLT PET. Target inhibition as measured by p-MEK levels was observed. However, similar to in vitro studies, PLX4720-treated COLO 205 xenografts exhibited elevated p-ERK & p-rpS6 protein levels relative to vehicle controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241616030.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241616030.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241616031.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-31020241616031.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - Deletion of Phb1 affects lipid metabolism.Western blot (a) & quantification (b) of Acetyl-CoA carboxylase (ACC) & phosphorylated ACC (p-ACC) expression at P20. N = 6–7 animals per genotype. Unpaired two-tailed t-test [p-ACC (t = 0.4627, df = 11, p = 0.021), ACC (t = 1.355, df = 11, p = 0.26)]. Western blot (c) & quantification (d) of ACC & p-ACC expression at P40. N = 6–8 animals per genotype [p-ACC (t = 0.5447, df = 12, p = 0.42), ACC (t = 1.153, df = 12, p = 0.17)]. Unpaired two-tailed t-test. By RT-qPCR, we identified a significant downregulation of many enzymes involved with lipid biosynthesis at both P20 (e) & P40 (f): sterol regulatory element-binding protein 1 (Srebp1), 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), ATP citrate lyase (Acly), fatty acid synthase (FASN), acetyl-CoA carboxylase 2 (ACC2), N = 5 animals per genotype. Unpaired two-tailed t-test P20 [Srebp1 (t = 3.26, df = 8, p = 0.012), Hmgcr (t = 7.63, df = 8, p = 0.000061), Acly (t = 4.418, df = 8, 0.0022), FASN (t = 4.109, df = 8, p = 0.0034), ACC2 (t = 3.408, df = 8, p = 0.0092)]; P40 [Srebp1 (t = 7.551, df = 8, p = 0.000066), Hmgcr (t = 5.091, df = 8, p = 0.00094), Acly (t = 4.934, df = 8, p = 0.0011), FASN (t = 7.186, df = 8, p = 0.000094), ACC2 (t = 2.697, df = 8, p = 0.027)]. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. n.s. non-significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34078899), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416175297.jpg" data-srcset="https://resources.bio-techne.com/images/products/nb600-936_rabbit-polyclonal-beta-tubulin-antibody-310202416175297.jpg" alt="beta Tubulin Antibody - BSA Free" title="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -" data-caption="Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - TK1 protein levels do not reflect p-ERK attenuation following inhibition of V600EBRAF inhibition in COLO 205 cells.COLO 205 cells were collected 48 hours of PLX 4032 exposure at 10 nM, 100 nM, 500 nM, 1 µM, or 5 µM. (A) Western blot analysis demonstrated target inhibition of p-MEK despite increased p-ERK levels. PI3K-mTOR signaling was elevated in a PLX 4032-dependent manner as exhibited by a steady rise in p-rpS6 levels. The ERK-phosphatase DUSP6 decreased in conjunction with mTOR signaling & was inversely proportional to p-ERK levels. A slight increase in p27 levels were observed concomitantly with only modest changes in TK1 levels, except at the highest dose of PLX4032. (B) Decreased TK1 mRNA levels were observed at all drug concentrations above 10 nM (p<0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Western Blot" data-header="beta Tubulin Antibody - BSA Free, Catalog # NB600-936" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] -</h4> Western Blot: beta Tubulin Antibody - BSA Free [NB600-936] - TK1 protein levels do not reflect p-ERK attenuation following inhibition of V600EBRAF inhibition in COLO 205 cells.COLO 205 cells were collected 48 hours of PLX 4032 exposure at 10 nM, 100 nM, 500 nM, 1 µM, or 5 µM. (A) Western blot analysis demonstrated target inhibition of p-MEK despite increased p-ERK levels. PI3K-mTOR signaling was elevated in a PLX 4032-dependent manner as exhibited by a steady rise in p-rpS6 levels. The ERK-phosphatase DUSP6 decreased in conjunction with mTOR signaling & was inversely proportional to p-ERK levels. A slight increase in p27 levels were observed concomitantly with only modest changes in TK1 levels, except at the highest dose of PLX4032. (B) Decreased TK1 mRNA levels were observed at all drug concentrations above 10 nM (p<0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25247710), licensed under a CC-BY license. Not internally tested by Novus Biologicals. </div> </div> </span> <div class="row"> <div class="col-md-2"> <a id="scientific-view-more" class="more"> View 22 more images </a> </div> </div> </div> </div> <div id="applications" class="py-5 bt-pp-border-bottom" data-anchor-label="Applications" data-section="section-antibody--applications"> <h3>Applications for beta Tubulin Antibody - BSA Free </h3> <div class="mt-5 py-2 container"> <div class="row"> <div class="col-lg-3 border font-weight-bold text-bg-dark py-2 align-top text-md-left text-white bg-dark-bt-blue d-none d-lg-block"> Application </div> <div class="col-lg-9 border align-top py-2 font-weight-bold text-white bg-dark-bt-blue d-none d-lg-block"> Recommended Usage </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunocytochemistry/ Immunofluorescence</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:500 - 1:1000 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunohistochemistry</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:1000 - 1:2000 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Immunohistochemistry-Paraffin</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:1000 - 1:2000 </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Simple Western</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:1000. Use reported in scientific literature (PMID 35008713) </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Western Blot</h4> </div> <div class="col-lg-9 border align-top py-2"> 1:1000 </div> </div> </div> <div id="application-notes" class="mt-5"> <div class="border-0 font-weight-bold"> Application Notes </div> <div class="product-data-wrapper"> In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. SCW validated using murine pancreatic cancer cells. </div> </div> <h4 class="mt-3">Reviewed Applications</h4> <p><span class="review_stars review_stars_4"></span> Read <a href="#reviews" class="reviews">7 reviews</a> rated 4.4 using NB600-936 in the following applications:</p> <ul class="list-unstyled"> <li class="py-1"><a href="#reviews" class="reviews" data-application="Immunocytochemistry">Immunocytochemistry (1 Review) </a></li> <li class="py-1"><a href="#reviews" class="reviews" data-application="Immunohistochemistry">Immunohistochemistry (1 Review) </a></li> <li class="py-1"><a href="#reviews" class="reviews" data-application="Simple Western">Simple Western (1 Review) </a></li> <li class="py-1"><a href="#reviews" class="reviews" data-application="Western Blot">Western Blot (4 Reviews) </a></li> </ul> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-base--preparation-storage"> <h3>Formulation, Preparation, and Storage</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purification</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Immunogen affinity purified </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Formulation</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> PBS </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Format</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> BSA Free </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Preservative</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 0.02% Sodium Azide </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Concentration</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> 1.0 mg/ml </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Shipping</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Stability & Storage</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: beta Tubulin</h3> <article class="product-data-wrapper"> The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments, and tubulins comprises the microtubule building block. Tubulin is a globular dimeric protein of alpha/beta chains and it has five distinct forms labeled as -a, -b,-g, -d and -e tubulin. a/b tubulins generates heterodimers which multimerize to form microtubule filaments. Several b tubulin isoforms such as b1, b2, b3, b4, b5, b6 , b8 etc have been identified among which b1/b4 are present in cytosol and b2 is present in nuclei/nucleoplasm. d/e tubulins are associated with centrosomes, whereas the g tubulin forms the gammasome, which is required for nucleating microtubule filaments at centrosomes. Several cellular movements are mediated by microtubule action which includes the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, nerve-cell axon migration etc and these movements result from competitive microtubule polymerization/depolymerization or through the actions of microtubule motor proteins. </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Long Name</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> Tubulin beta chain </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> OK/SW-cl.56, TUBB, TUBB5 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Entrez Gene IDs</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.ncbi.nlm.nih.gov/gene?term=203068" class="a___link-external" target="_blank" rel="nofollow">203068</a> (Human); <a href="https://www.ncbi.nlm.nih.gov/gene?term=545486" class="a___link-external" target="_blank" rel="nofollow">545486</a> (Mouse); <a href="https://www.ncbi.nlm.nih.gov/gene?term=498736" class="a___link-external" target="_blank" rel="nofollow">498736</a> (Rat) </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Gene Symbol</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> TUBB </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>UniProt</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.uniprot.org/uniprot/?query=P07437" class="a___link-external" target="_blank" rel="nofollow">P07437 (Human)</a> </div> </div> </div> <h4 class="mt-3">Additional beta Tubulin Products</h4> <ul class="list-unstyled"> <li class="py-1"><a href="/t/beta-tubulin" data-product="beta Tubulin">All Products for beta Tubulin </a></li> <li class="py-1"><a href="/t/beta-tubulin/elisa-kits" data-application="beta Tubulin ELISA Kits">beta Tubulin ELISA Kits </a></li> <li class="py-1"><a href="/t/beta-tubulin/lysates" data-application="beta Tubulin Lysates">beta Tubulin Lysates </a></li> <li class="py-1"><a href="/t/beta-tubulin/antibodies" data-application="beta Tubulin Primary Antibodies">beta Tubulin Primary Antibodies </a></li> <li class="py-1"><a href="/t/beta-tubulin/proteins-enzymes" data-application="beta Tubulin Proteins and Enzymes">beta Tubulin Proteins and Enzymes </a></li> </ul> </div> <div id="product-documents" data-section="section-base--product-documents"> <div 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Western Blot Protocol</a></p> </div> <div class="col-md-4 datasheet-pdf-result"> </div> </div> </div> </div> <div class="card-header"> <a data-toggle="collapse" data-target="#collapse_one" aria-expanded="false" class="collapsed" aria-controls="collapse_one"> <h5 class="mb-0"> COA </h5> </a> </div> <div id="collapse_one" class="collapse " aria-labelledby="heading_one" data-parent="#accordion"> <div class="card-body"> <form novalidate="novalidate" data-drupal-selector="product-coa-form" action="#coa-form" method="post" id="product-coa-form" accept-charset="UTF-8"> <div class="row"> <div class="col-12"> <h2>Certificate of Analysis</h2> <p>To download a Certificate of Analysis, please enter a lot number in the search box below.</p> </div> </div> <section class="row"> <div class="col-md-6"> <form novalidate="novalidate" data-drupal-selector="product-coa-form"> <div class="form-row align-items-center"> <div class="col-md-10 my-1"> <input 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Primary Antibodies are guaranteed for 1 year from date of receipt.</p> </div> </div> <div class="tab-pane fade" id="citations_reviews" role="tabpanel" aria-labelledby="citations_reviews-tab"> <div data-section="section-base--technical-anchors"> <ul class="product-pages-anchors nav mt-2"> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor active" href="#citations"> Citations</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#reviews"> Reviews</a> </li> </ul> </div> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="NB600-936"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> <section id="reviews-tab-content-placeholder" class="tab-content-placeholder" data-id="reviews" data-product_code="nb600-936"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span 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