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</figure> <div class="text">Partner with us for your Biomarker Discovery & Clinical Trials</div> </div></a> <ul class="sub-menu"> <li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-2971"><a href="https://www.almacgroup.com/diagnostics/our-business-development-team/">Our BD Team</a></li> <li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-157"><a href="https://www.almacgroup.com/diagnostics/supporting-services/our-laboratories/">Laboratory Locations</a></li> <li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-173"><a href="https://www.almacgroup.com/diagnostics/newsletter-signup/">Sign up for our newsletter</a></li> <li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-155"><a href="https://www.almacgroup.com/diagnostics/contact-us/">Contact Us</a></li> </ul> </li> </ul> </li> </ul> </aside> </div> <div class="o-grid__item u-width-9/12 u-width-8/12@l u-width-full@s u-p-l4 u-p-l2@l u-p-l1@m u-p-b4 s-cms u-order-2@s u-overflow-hidden">  <h2>RNA-Sequencing Solutions</h2>  <script type="IN/Form2"></script> <h2 class="wp-block-heading has-medium-font-size">Background Information</h2> <p>Almac Diagnostic Services has a proven ability to discover, develop and validate high quality, innovative RNA-based biomarkers with an extensive track record in establishing assays across key regulatory standards. We currently offer multiple Research Use Only (RUO) RNA-Sequencing solutions analysed using the Illumina NextSeq500, NextSeq550, NovaSeq6000 and NovaSeq X Plus <strong><a href="/diagnostics/supporting-services/platforms-technologies/">sequencing platforms</a></strong>.  All our RNA-Sequencing solutions support novel <strong><a href="https://www.almacgroup.com/diagnostics/genomic-services">biomarker discovery</a></strong> and translational research, supported by Almac innovative proprietary <a href="/diagnostics/supporting-services/bioinformatics-biostatistics-software-development/"><strong>bioinformatics</strong></a> solutions. These include:</p> <div style="height:35px" aria-hidden="true" class="wp-block-spacer"></div> <div style="overflow: auto;"> <div class="u-hidden@s" style="width: 690px;"><img decoding="async" class="aligncenter lazyload" data-src="/diagnostics/wp-content/uploads/sites/3/2020/03/rna-chart-hotspot.svg" usemap="#image-map-desktop" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw=="><noscript><img decoding="async" class="aligncenter" src="/diagnostics/wp-content/uploads/sites/3/2020/03/rna-chart-hotspot.svg" usemap="#image-map-desktop"></noscript> <map name="image-map-desktop"> <area title="RNA-Sequencing Solutions" alt="RNA-Sequencing Solutions" coords="169,130,-1,73" shape="rect" href="#rna-sequencing" target=""> <area title="Illumina RNA Exome" alt="Illumina RNA Exome" coords="205,-1,367,55" shape="rect" href="/diagnostics/rna-panel-solution/" target=""> <area title="Roche Total RNASeq" alt="Roche Total RNASeq" coords="205,73,366,130" shape="rect" href="#roche" target=""> <area title="Illumina mRNA Stranded" alt="Illumina mRNA Stranded" coords="205,147,368,202" shape="rect" href="#illumina-mrna" target=""> <area title="ClaraT Total mRNA Report" alt="ClaraT Total mRNA Report" coords="411,36,593,167" shape="rect" href="/diagnostics/claratreport/" target=""> </map> </div> </div> <div class="u-hidden u-block@s"><img decoding="async" data-src="https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/rna-chart-hotspot-mobile.svg" usemap="#image-map-mobile" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload"><noscript><img decoding="async" src="https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/rna-chart-hotspot-mobile.svg" usemap="#image-map-mobile"></noscript> <map name="image-map-mobile"> <area title="RNA-Sequencing Solutions" alt="RNA-Sequencing Solutions" coords="61,0,235,56" shape="rect" href="#rna-sequencing" target=""> <area title="Illumina RNA Exome" alt="Illumina RNA Exome" coords="-1,91,94,177" shape="rect" href="#illumina" target=""> <area title="Roche Total RNASeq" alt="Roche Total RNASeq" coords="101,93,195,177" shape="rect" href="#roche" target=""> <area title="Illumina mRNA Stranded" alt="Illumina mRNA Stranded" coords="203,93,295,175" shape="rect" href="#illumina-mrna" target=""> <area title="ClaraT Total mRNA Report" alt="ClaraT Total mRNA Report" coords="70,194,222,296" shape="rect" href="/diagnostics/claratreport/" target=""> </map> </div> <div style="height:47px" aria-hidden="true" class="wp-block-spacer"></div> <h4 class="wp-block-heading" id="h-to-find-out-more-information-on-each-almac-solution-click-the-appropriate-drop-down-below">To find out more information on each Almac solution, click the appropriate drop-down below:</h4>  <section class="u-bg-white u-p-y4 t-theme-component-overwrite" id="accordionBlock"> <div class="o-wrapper"> <div class="o-grid o-grid--center"> <div class="o-grid__item u-width-full u-align-center u-align-left@m"> </div> <div class="o-grid__item u-width-full"> <div class="u-m-t2"> <div class="c-accordion js-accordion"> <article id="item1" class="c-accordion__item u-c-mid-grey accordion-1"> <h4 class="c-accordion__title u-bg-white u-c-mid-grey js-accordionTarget-one">RNA Exome Sequencing</h4> <div class="c-accordion__content u-bg-white"> <div class="o-grid">   <div class="o-grid__item u-width-full"> <div class="s-cms"> <p><strong><span style="color: #7030a0;">RNA Exome Sequencing</span></strong></p> <p><strong>The Platform</strong>: RNA Exome is a probe-based target capture approach for sequencing of coding regions. An <strong>Almac optimised solution</strong> recommended for <strong>gene expression</strong> from poor quality and formalin-fixed paraffin embedded <strong>(FFPE) samples</strong>.</p> <p><strong><span style="color: #7030a0;">Features & Benefits:</span></strong></p> <ul> <li><strong>Comprehensive panel</strong> covering >21,000 genes from the RefSeq Exome (99%)</li> <li>Almac optimised process and workflow <strong>maximising on pass rates and sequencing data quality</strong> from challenging sample types</li> <li>Produces <strong>highly repeatable and reproducible </strong>gene expression data in comparison to other RNA expression platforms</li> <li>Almac have <strong>analytically validated</strong> a selection of gene expression signatures demonstrating RNA Exome as a <strong>potential platform suitable for CTA delivery</strong></li> </ul> <div class="c-diag-table-wrap"> <table> <tbody> <tr> <th style="background-color: #7030a0;">Metric</th> <th style="background-color: #7030a0;">RNA Exome Solution</th> </tr> <tr> <td>Chemistry</td> <td>Illumina TruSeq™ RNA Exome</td> </tr> <tr> <td>Tissue Type</td> <td>Optimised for FFPE (compatible with other sample types on request)</td> </tr> <tr> <td>Recommended Tissue Requirements</td> <td>For FFPE, tissue should be macrodissected<br /> 10 x 5 µm FFPE sections with a tissue area >1cm<sup>2</sup> and high tumour content >10% viable cells<br /> Specific guidelines will be provided on a per-project basis</td> </tr> <tr> <td>Recommended RNA Input</td> <td>Recommended Minimum 100 ng<br /> (100-500 ng dependent on quality based on DV<sub>200</sub>)</td> </tr> <tr> <td>Standard Sequencing Specification</td> <td>25M PE reads (50M Total Reads)<br /> <span style="font-family: inherit; font-size: inherit;">Other Sequencing specifications available on request</span></td> </tr> <tr> <td>Deliverables</td> <td>FASTQ, FPKM Expression Matrix, Count Matrix and QC Report as standard. BAM files on request</td> </tr> </tbody> </table> <h6><strong>NOTE:</strong> Optimal sample number ≥24 samples</h6> </div> <p style="text-align: center;"><span style="font-family: 'trebuchet ms', geneva; font-weight: bold;"><a style="color: #ffffff; background: #7030a0; text-decoration: none; border: 2px solid #7030a0; border-radius: 4px; padding: 8px 14px; font-size: 18px;" href="/diagnostics/rna-panel-solution/" rel=" noopener">Find out more</a></span></p> <p> </p> </div> </div> </div> </article> <article id="item2" class="c-accordion__item u-c-mid-grey accordion-2"> <h4 class="c-accordion__title u-bg-white u-c-mid-grey js-accordionTarget-one">Total RNA-Sequencing</h4> <div class="c-accordion__content u-bg-white"> <div class="o-grid">   <div class="o-grid__item u-width-full"> <div class="s-cms"> <p><strong><span style="color: #7030a0;">Total RNA-Sequencing</span></strong></p> <p><strong>The Platform</strong>: Total RNA-Seq is a ribosomal depletion approach for <strong>sequencing of both coding and non-coding regions</strong>. A total assessment specifically suitable for including an evaluation of non-coding regions in either <strong>fresh frozen (FF) or FFPE samples</strong>. Also includes in-built globin depletion for analysis of whole blood samples.</p> <p><strong><span style="color: #7030a0;">Features & Benefits:</span></strong></p> <ul> <li>Complete workflow with <strong>efficient ribosomal RNA (rRNA) depletion</strong> steps in-built</li> <li><strong>Lower duplication rates</strong> observed in samples resulting in increased sequencing success</li> <li>Additional downstream applications by targeting non-coding RNA</li> </ul> <div class="c-diag-table-wrap"> <table> <tbody> <tr> <th style="background-color: #7030a0;">Metric</th> <th style="background-color: #7030a0;">Total RNA-Seq Solution</th> </tr> <tr> <td>Chemistry</td> <td>Roche KAPA HyperPrep Kit with RiboErase (HMR)</td> </tr> <tr> <td>Tissue Type</td> <td>FF or FFPE (compatible with other sample types on request)</td> </tr> <tr> <td>Recommended Tissue Requirements</td> <td> <p>For FFPE, tissue should be macrodissected<br /> 10 x 5 µm FFPE sections with a tissue area >1cm<sup>2</sup> and high tumour content >10% viable cells<br /> Specific guidelines will be provided on a per-project basis</p> </td> </tr> <tr> <td>Recommended RNA Input</td> <td>Recommended Minimum 100 ng</td> </tr> <tr> <td>Standard Sequencing Specification</td> <td> <p>50M PE reads (100M Total Reads) for all applications<br /> Other Sequencing specifications available on request</p> </td> </tr> <tr> <td>Deliverables</td> <td>FASTQ, FPKM Expression Matrix, Count Matrix and QC Report as standard. BAM files on request</td> </tr> </tbody> </table> <h6><strong>NOTE:</strong> Optimal sample number ≥24 samples</h6> </div> </div> </div> </div> </article> <article id="item3" class="c-accordion__item u-c-mid-grey accordion-3"> <h4 class="c-accordion__title u-bg-white u-c-mid-grey js-accordionTarget-one">mRNA Stranded Sequencing</h4> <div class="c-accordion__content u-bg-white"> <div class="o-grid">   <div class="o-grid__item u-width-full"> <div class="s-cms"> <p><strong><span style="color: #7030a0;">mRNA Stranded Sequencing</span></strong></p> <p><strong>The Platform</strong>: mRNA Stranded Sequencing is a polyA stranded capture approach for <strong>transcriptome sequencing including antisense analysis</strong> of good quality <strong>FF samples only</strong>.</p> <p><strong><span style="color: #7030a0;">Features & Benefits:</span></strong></p> <ul> <li>Captures a <strong>complete overview of the coding transcriptome with strand information</strong></li> <li>A <strong>broad dynamic range</strong> to enable accurate gene expression changes in good quality samples</li> <li>Provides an understanding of both <strong>known and novel RNA fusions</strong> in FF samples</li> </ul> <div class="c-diag-table-wrap"> <table> <tbody> <tr> <th style="background-color: #7030a0;">Metric</th> <th style="background-color: #7030a0;">mRNA Stranded Solution</th> </tr> <tr> <td>Chemistry</td> <td>Illumina TruSeq™ Stranded mRNA</td> </tr> <tr> <td>Tissue Type</td> <td>FF only</td> </tr> <tr> <td>Recommended Tissue Requirements</td> <td> <p>Specific tissue guidelines will be provided on a per-project basis</p> </td> </tr> <tr> <td>Recommended RNA Input</td> <td>Recommended Minimum 100 ng</td> </tr> <tr> <td>Standard Sequencing Specification</td> <td>30M SE reads (30M Total Reads)</td> </tr> <tr> <td>Deliverables</td> <td>FASTQ, FPKM Expression Matrix, Count Matrix and QC Report as standard. BAM files on request.</td> </tr> </tbody> </table> <h6><strong>NOTE:</strong> Optimal sample number ≥24 samples</h6> </div> </div> </div> </div> </article> </div>  </div> </div> </div>  </div>  </section> <div style="height:32px" aria-hidden="true" class="wp-block-spacer"></div> <h2 class="wp-block-heading" style="font-size:26px">Almac Additional Benefits for RNA Sequencing Solutions</h2> <p>Flexible Extraction Protocols: Almac offer both high quality <strong>single RNA extractions and a dual RNA/DNA extraction protocol</strong> for our RNA-Sequencing solutions. Dual extraction facilitates simultaneous generation of RNA-Seq data and <a href="/diagnostics/dna-sequencing-solutions/"><strong style="color:#7030a0;">DNA-Seq</strong></a> data from a single sample, <strong>conserving valuable sample material</strong>.</p> <p>Customisable Analysis & Reporting: Data analysis and reporting is performed using <strong>Almac customised</strong> <strong><a href="/diagnostics/supporting-services/bioinformatics-biostatistics-excellence/"> bioinformatics</a> <strong>pipelines</strong> implemented on <strong>DNAnexus</strong>, a global leader in biomedical informatics and data management. DNAnexus is a <strong>cloud-based NGS solution</strong> which ensures scalability and security of project data. A significant benefit of utilising DNAnexus within this offering is the <strong>flexibility of workflow optimisation</strong> and the <strong>ability to customise bespoke pipelines</strong> for quality control, alignment & expression estimation.</strong></p> <div class="wp-block-image"> <figure class="alignright size-large"><a href="https://www.almacgroup.com/diagnostics/claratreport/"><img decoding="async" width="192" height="135" data-src="https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/claraT-logo-1.png" alt="" class="wp-image-1262 lazyload" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" style="--smush-placeholder-width: 192px; --smush-placeholder-aspect-ratio: 192/135;" /><noscript><img decoding="async" width="192" height="135" src="https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/claraT-logo-1.png" alt="" class="wp-image-1262"/></noscript></a></figure></div> <p>clara<sup>T</sup> – Unique gene expression report: <a href="/diagnostics/claratreport/">clara<sup>T</sup></a> classifies <strong>multiple publicly available gene expression signatures</strong> and <strong>single gene targets</strong> linked to <strong>multiple key biologies</strong>, alongside Almac’s own proprietary assays, according to the well-established 10 <strong>Hallmarks of Cancer</strong>. The clara<sup>T</sup> report is a pan-cancer solution generated from raw gene expression data, utilising Almac’s RNA-Sequencing Services. </p> <div style="height:50px" aria-hidden="true" class="wp-block-spacer"></div> <h2 class="wp-block-heading" style="font-size:26px">RNA-Sequencing Resources</h2> <p>To view additional resources such as whitepapers, technical factsheets, webinars and other information, <strong><a href="#relatedResourcesScroll" style="color:#7030a0;">click here</a></strong>.</p> <div style="height:50px" aria-hidden="true" class="wp-block-spacer"></div> <h2 class="wp-block-heading" style="font-size:25px">Contact Us</h2> <p>Interested in partnering with Almac on your biomarker project using our RNA Sequencing Solutions? Get in touch below:</p> <div class="almac-hubspot-form"></div> <script type="lazyload" src="//js.hsforms.net/forms/v2.js" charset="utf-8"></script> <script type="lazyload">hbspt.forms.create({"portalId":742105,"formId":"fb28842d-1f6c-4c23-a2df-bf58af6e5c68","target":".almac-hubspot-form","css":""});</script> <div id="relatedResourcesScroll"></div> <article class="o-grid o-grid--full"> <div class="o-grid__item u-width-full u-m-t6 u-m-t4@m u-m-b4"> <h2 class="u-normal u-a-center">Related Resources</h2> </div> <div class="u-flex u-flex-justify-center u-flex-wrap u-width-full c-news-panels__slider js-news-panels"> <article class="u-width-1/4 u-width-half@l u-width-full@m"> <a href="https://www.almacgroup.com/diagnostics/diagnostic-resource/overcoming-the-challenges-of-taking-rna-biomarkers-into-clinical-trials/"> <div class="c-news-panels u-a-center"> <div class="c-news-panels__img lazyload" style="background-image:inherit;" data-bg-image="url('https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/04/clarat-exome-bg.jpg')"> <p class="u-text-meta">Webinar</p> </div> <div class="c-news-panels__content u-width-full u-p-x2 u-p-y2 u-bg-white"> <p class="u-text-18 u-normal">Overcoming the Challenges of Taking RNA Biomarkers into Clinical Trials</p> </div> </div> </a> </article> <article class="u-width-1/4 u-width-half@l u-width-full@m"> <a href="/wp-content/uploads/2020/03/ALMAC-Diagnostics-RNA-Fact-Sheet-NEW-VERSION-UPDATED-with-New-QC-Metrics-Mar-2020V2.pdf"> <div class="c-news-panels u-a-center"> <div class="c-news-panels__img lazyload" style="background-image:inherit;" data-bg-image="url('https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/Almac-Diagnostics-RNA-panel-solution-1.jpg')"> <p class="u-text-meta">Technical Sheet</p> </div> <div class="c-news-panels__content u-width-full u-p-x2 u-p-y2 u-bg-white"> <p class="u-text-18 u-normal">RNA Exome Technical Specification</p> </div> </div> </a> </article> <article class="u-width-1/4 u-width-half@l u-width-full@m"> <a href="/wp-content/uploads/2020/03/21314-ALMAC-Diagnostics-Online-White-Paper-Mar-2020_v2.pdf"> <div class="c-news-panels u-a-center"> <div class="c-news-panels__img lazyload" style="background-image:inherit;" data-bg-image="url('https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/Almac-Diagnostics-RNA-panel-solution-1.jpg')"> <p class="u-text-meta">White paper</p> </div> <div class="c-news-panels__content u-width-full u-p-x2 u-p-y2 u-bg-white"> <p class="u-text-18 u-normal">RNA Exome Whitepaper</p> </div> </div> </a> </article> <article class="u-width-1/4 u-width-half@l u-width-full@m"> <a href="/diagnostics/claratreport/rna-blog/"> <div class="c-news-panels u-a-center"> <div class="c-news-panels__img lazyload" style="background-image:inherit;" data-bg-image="url('https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/Almac-Diagnostics-RNA-panel-solution-1.jpg')"> <p class="u-text-meta">Almac Voice</p> </div> <div class="c-news-panels__content u-width-full u-p-x2 u-p-y2 u-bg-white"> <p class="u-text-18 u-normal">RNA-Seq Blog</p> </div> </div> </a> </article> <article class="u-width-1/4 u-width-half@l u-width-full@m"> <a href="https://rochesequencingstore.com/catalog/kapa-rna-hyperprep-kit-with-riboerase-hmr/"> <div class="c-news-panels u-a-center"> <div class="c-news-panels__img lazyload" style="background-image:inherit;" data-bg-image="url('https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/Almac-Diagnostics-RNA-panel-solution-1.jpg')"> <p class="u-text-meta">Technical Sheet</p> </div> <div class="c-news-panels__content u-width-full u-p-x2 u-p-y2 u-bg-white"> <p class="u-text-18 u-normal">Total RNA-Seq Technical Specification</p> </div> </div> </a> </article> <article class="u-width-1/4 u-width-half@l u-width-full@m"> <a href="https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-stranded-mrna.html?langsel=/us/"> <div class="c-news-panels u-a-center"> <div class="c-news-panels__img lazyload" style="background-image:inherit;" data-bg-image="url('https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/Almac-Diagnostics-RNA-panel-solution-1.jpg')"> <p class="u-text-meta">Technical Sheet</p> </div> <div class="c-news-panels__content u-width-full u-p-x2 u-p-y2 u-bg-white"> <p class="u-text-18 u-normal">mRNA Stranded Technical Specification</p> </div> </div> </a> </article> <article class="u-width-1/4 u-width-half@l u-width-full@m"> <a href="/diagnostics/claratreport/press-release/"> <div class="c-news-panels u-a-center"> <div class="c-news-panels__img lazyload" style="background-image:inherit;" data-bg-image="url('https://www.almacgroup.com/diagnostics/wp-content/uploads/sites/3/2020/03/Almac-Diagnostics-RNA-panel-solution-1.jpg')"> <p class="u-text-meta">Article</p> </div> <div class="c-news-panels__content u-width-full u-p-x2 u-p-y2 u-bg-white"> <p class="u-text-18 u-normal">claraT Press Release</p> </div> </div> </a> </article> </div> </article>  <style> .s-cms.s-cms--diagnostics p a { 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