CINXE.COM

<!DOCTYPE html><html lang="en-US"><head><title>Just a moment...</title><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><meta http-equiv="X-UA-Compatible" content="IE=Edge"><meta name="robots" content="noindex,nofollow"><meta name="viewport" content="width=device-width,initial-scale=1"><style>*{box-sizing:border-box;margin:0;padding:0}html{line-height:1.15;-webkit-text-size-adjust:100%;color:#313131;font-family:system-ui,-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Helvetica Neue,Arial,Noto Sans,sans-serif,Apple Color Emoji,Segoe UI Emoji,Segoe UI Symbol,Noto Color Emoji}body{display:flex;flex-direction:column;height:100vh;min-height:100vh}.main-content{margin:8rem auto;max-width:60rem;padding-left:1.5rem}@media (width <= 720px){.main-content{margin-top:4rem}}.h2{font-size:1.5rem;font-weight:500;line-height:2.25rem}@media (width <= 720px){.h2{font-size:1.25rem;line-height:1.5rem}}#challenge-error-text{background-image:url(data:image/svg+xml;base64,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);background-repeat:no-repeat;background-size:contain;padding-left:34px}@media (prefers-color-scheme:dark){body{background-color:#222;color:#d9d9d9}}</style><meta http-equiv="refresh" content="390"></head><body class="no-js"><div class="main-wrapper" role="main"><div class="main-content"><noscript><div class="h2"><span id="challenge-error-text">Enable JavaScript and cookies to continue</span></div></noscript></div></div><script>(function(){window._cf_chl_opt={cvId: '3',cZone: "pubs.acs.org",cType: 'managed',cRay: '8e9e5640ac1fcdea',cH: 'xVJYQZcqUfOAtRGjiJQp2oUoaZ_2xMdofpyPEAZ1idw-1732838630-1.2.1.1-NWX7sYJR3EKJubB1JUPgs.7UJIgLIW5iW2Tj14C.rGdnTre6TJPFp3_Uz0HHFEn7',cUPMDTk: "\/doi\/10.1021\/bi0020067?__cf_chl_tk=TI76maBnIZeuNGNEL.gNJHw.piPRsbF0ybmlIET8EQs-1732838630-1.0.1.1-yokRjDBP31cKXW_rQGcuWCxZ.Zj10SqHNdR6Wh0.ysM",cFPWv: 'b',cITimeS: '1732838630',cTTimeMs: '1000',cMTimeMs: '390000',cTplC: 0,cTplV: 5,cTplB: 'cf',cK: "",fa: "\/doi\/10.1021\/bi0020067?__cf_chl_f_tk=TI76maBnIZeuNGNEL.gNJHw.piPRsbF0ybmlIET8EQs-1732838630-1.0.1.1-yokRjDBP31cKXW_rQGcuWCxZ.Zj10SqHNdR6Wh0.ysM",md: "OsngSxgDMih5Uxg0PIsvZiVdSyMUYf1c2zD6JoSfECg-1732838630-1.2.1.1-Uu0UZchyXXDCXpwNPJs7Tn1e0u_a_0JLD1TXKHD.7PbomvEqJWfi4TV0oavecjMq2KGPdErDcMRDoVF.7oBjk7abcptSgm8ws2QcLIWd6LZ.TSdl_ZjF52vxm2hygmcJ.bRFu0iUee_EE0ce30gxVE5VnLusz7Ajfi2mbDzjr2VqqNrmpgwbq4yefbi97EyhV9iN8TQUH4q3YOZjkhtpNTiBlIOgPHdyzJn6rEaZi7eBXz8X7oYgbtrYk5j9.NTd5feRTyDdIMtPIowcs4_eFRezg8.kYrmkDzc_UtBt01Sp2Hli4RXTB3Er6rNK4aXNVlUtOwduD3yr_u1US8C7S5mHLKXAtn_J0IyFmQdamCulZKJsFFzRh04j7lmZEkEz36u1zRtCWGpAAa5dK_uAlcilnq_.zC_mspG8_NIdzMr1PUQC2LLZRmhxkCgXqAof6klohMjoBLzN5qZwxBgtcS6_8AlSSo0QnlNN4O0STPEGGYiORGS1wN11eRKy6.hd9m7qBEK2sA8Wv76gPN_8xnDuTLt10kHiUZx3D2F4hjCweJY4vYP2Hd2OIBJmFF2c3HupoIrTxbQo0WK5tj9BW3H3EmTufI0GgXQ_.3RRRoKJHqIOdKmhzLWB7EeprrpxGGPKzKBBfCQgIBmcFG9ke6OdRmEDkVA39xOPdWV3gc_0UyY4LdhMx4n7O38yX74mI56G3XyyyCfZzCtufRR.wMIhwvgOx2FXWZZS9.0Ky5Koit3TQIWLEM5PV8zzxF2eeLjWW0ro5Ns8xqDvad9qj68jKnAO0AcNXUnNOknr8ltm9_R7pxhqKRxY_kz7PmcijCqUDI9j37.zi.O5qFQwqzeY3uWeYPFjol16JtKf.LOpJKBcCPE69AAGHkyVIG5HgWUxmTNz3d1_v7UR__1Od_jk8iEw5Yn48fM6MxiTNsdolDFLISjACCZQSNx06XR.p18SdHUAM8.rpSFzkceSWQDfjWoXEToa_En3ta.qBrgBJ3FnMMXTTD5Q0TZnYHLzh0pjNOL4SFk3zD5TJcZxKBAUI.gVZkwFSTMkJ4CqgE0FVdgEzVxkLLW5nk8t4Mzi6dselrV3oHlkInEtJHoYUCHkrfRWhCnt4hZ_8Fu_RiqZc.j6gCwIYrLbKRseoN4i8_XqLJrx4GlEOeeAo1m.ZJRacjnRRe0Qx3uJ.6lmRSvxXzw8cEwlEr2moSPvIH.WiBpcuNgNdK966s2GbG5Hx1.0U2XzJ7G8ssCNp6Zao.eoRy_gJKDGUQVXJW.ymetCFisqWVYwH1zCZ.TLAubD.L_vh65a5uW6jvhPZ26LG9CHLB5qBSivWwaag4qG5XXoixopf8EEIn5wwsSboM8AzrhSgwOEE2rlc9rv5c8HVuo78SZIILwPJm8KLoILcmH5SeM_yhchJa.DgfrRURD2BqiN3CrxNseULX.7AK3Vc.xm9P88DR5UOwmW1AiUUiQ7fjq4noIFOmTDBADFSgAPJ3ZI.nNXMqMyV6.QsSEcW6KzWBoCQMjzn9OnJspyMMzeW4otvr_ABREUsVUap6M93l_xARvdxpbB81XrVMCeYpuJblRM3lcG9Lmh.loNsYL1gIx2ROIJ1cV1dyMCske86umK0SZAwaWsGmhjGufBpcvkqSJHTubYRWEhAZdIxz70UI8gLqT0mKfmCZHUlFxnBp5seDxb.Sx9yNjNacE3KipOQerscbSJM2jdknZVSGOwQSHT2mCw.vVNoCv7Us2leZPRQFgf0eCHZNJ7uqOj7pWhTj0aE4zfFzaRRBqjsYRSqDwoO7p54RrI3ZgEBvkdMuZ.cVnNVgQvQ_M.R4h0zeDMpUrSi1v00ZxrU3MxBfneF_uXnT6bz56Aeev.Q0T5J649H_IdAIydi8vIYQ7fXYIxr4VxPoUmSxd7yKp3dwvM3.ct.HVRtTjLf5BkfW0zBfJ9MBCE1CtYAt0eCBrw4iELRhKbEx3BH5gD1Zyx0NmWTy8hPppn6h518Dhp4muhCqTicjuGM.HQ.ZS3U_3VLR8k9xR6UdGW8NEjZm0CewQZDmnHAdSo0warF1QfPC2X6f5m_v6XByd.kKF3FIrmQU38xiSlm8MNr8UsoCwGjBG5G.QwlOLF52JpGyHO32mPAvM65qwqiWZesX5IHEnyWs8Lxsk6G1mCC.Vl3I1g2WKCgfbJLoOxFXqvgQ7xaebld9UtaiR8VRLvP3ZOtIjopxTGCiBuS.bkZBZJ8R.c4vgrymcsN9GKLHN4cfvoWP7YTBLjhVy8H54ybIFzUYza7OTcq7W9saPkKwM9VjAo8Gcrx67kBVClkxeB7Qs28gfRESZIf96h3iyDXwYXyPgHxFKnp48y4ronp2lEKXxynkZEXZWJ8LA0dC6m0C1tjj6HpNKTB43Tg_2Lx6FAETwB2lONPx3ufMsMUzHTMIlZir16EaTvWnYEZNN7cQNemGCN.yz53ZsZqauc_rjajxYlb4RZm.KnYvBRGMY3fxEZ1jxcPbc81RfUHTxu3c1lEzfyaHzpwbj4hV16m6WP9j30K1R4zhgxTcG5Y4saooKWtpV8VSHy6tjlaCd1ERR88EdbTr3pDGnAXHFbjdsp2TFI5U6P1b5vVBFDWl6DoI6505q1Qa6XeX6h_.moZZozVoumStKSqI17wcF27TiIDW.3RvpsyCSL.AM6CSe_mBXzaoRZwWh5_cvqhqS9vghXnA3fA4egMyTWicnaR.vTPnkpk9xdCM4wPOIWf8LsLF4AdRbU.sjv4bwpaL0aQnYXmXdmF4IgucTfkrJ2riJhAzESVWOVK6BaiXpZaz8t0mradRQHPjhXmgr2rVyueCJto2s7DLwGtecEBS7LbhLjguKw28wB375Vm9oLxqS7W5GSaZ.9_ShujvhySl.9WuBJIry31D63Ij9HSf0uGVGMx6CfsaCq30P_nM57RRmFuUNw3w0yvhEJnuBH1tBkO9z7mKhREg",mdrd: "b3e7Q82qgoWiPScS0qizi.ajpDV6s5Y2vl3pIbOEo8o-1732838630-1.2.1.1-.UdYWGXwLZXfvyWDkU2L1p30NvbWlvYt41aX1p06epOrApP8pR47dVPyYv2pw0XpmwI8goB1krlgUGga2gy9xasWumHmOHnMfyP9e5y7.srO3VPUzP1Aw3gqXPUd2lzW2UdRCb0CpZAue4PjI48c.ptVPF_HTrPG2xM3.xoqX2P1VlrQ5cH7dTjX4TwzBaF08EgYYnMZNKBKVXcyjdtQGv7wFBKv1m7q9EF.w9yCd3sLGjp64zrIigFTv_Nm6rm67HwlR1aDwicpk_A6kPGDA_CUlhrP_2WC1CWuFKwsGPIwl4bpRPLhrq.S06nKSxZ3oO1oJOzibF41TWt0Uo0EyVWnslDMtmZRr03P1xUMmpn5GJsMzagSZ9I4nlMhzn3FXSHTayNlf08Qe.tEOib5e8AC2kGtCTggmdTax2NfcI5bg8WWt1UKLjKuS99iFUIV97k.1vAPw6SQmYpZVRnPoBGgaEbw8xqSE1Hhv_5u.YiR5qYQnTEOyihGxuAdqEHAZEMmjTmL9t1p_L9KF55kc5inuKsUvE1efVNfzYqnZY0kCgmcoa.gd2rZUdGyxoPA2bMOlTd5IHS8aYbpFLGaeYOYkmDtdaHqNLulRBGRGrt2orR0p_X7kzoOKKEm0MtudufGDegONTK9SxzdNIDYGN9IezdfrK.7PLl.bEf5XmDCqw4kg53JrlSrZ6VhFOSIXE5.SRf0gbwf.CiMAGlD9pbeYCRFRViUBBnXm31MA4.zUXaxRb9o6gpzblTREA5HkpwqleIlKT6pYZlC1cSWU2zE0GrOzaB_8G01ch.PV6dTgGUnqRSW6kMjHZIJaCttf9wboa8DJbMK8rfwArkJahq75RdFRuo71LziWsrJRnCKqk1qTxEJM8A3MX8qY27LHHyOxEfNshikps94oU8G77pLT9bci1gUqPCPqXTHJIfJTBuhD3PCVWw_yPdg4KVXlsXxzMkvYG4SRyzOID.0NsS3XvdGAmzY13eZEMUUyu3qmFfsvD5o6qEM2gw3ybwwxOcVFU.CUL.ze4KlIWR9hAn9rvYwzln2Ml9T.bLymoowXNJx4Uo_Giww_6_8_y_Ir_xk8OgKCVOP.0w3E4h8f5lThVtxbFqe86MjCapymxVGWrdLCaIYc2mjP9CMT_Xxg.Z.1Cb8AqwrS_dtJcUHwfi8BMNPdBhlad8QYUUGCTET5ymoRu2PRixLzyx2vhxk4XrrXbosWaNuN_kWqdgW1_kfVYTM8PkOIjF_0JaEoMVUplDfb_jDUNYzXoV4JdLgY1_lRhyHigXMJUnXcgoZk5psdncp.Z2N1MQqAnE5qHGsPXY0ooxmw8RXmi2pLpqIHns4ZwtKhZIl3wEb5FSeLQcEQ0CS3gpllD7ZhV5cKE9y9VM5Hj2tx1s0H8nwyNlcZwNzb17wZoJZRvFnkaQWsI7WKezpGMReduVXHHC_.qecpd5yPRRHrSeRch18sYBJiKV3wM8hnsDZxN5Onob6kzaDCOf9c1a22I8H0.0t_fbQql.MZ9P_Qy3FFvP5.gcG3pWRA.wZdbCzBRkPURkPPVw13khfLsbFrZjvBVjpFfaRkx2fb3aoTNws25U_fY_OcLSYckOyEzs2kd_85B.aJbrvPPs.MPWifcGsNhCu7.jVIu6FiWfhN55D7sKSWX7tQuR1RwdKpsM.nNYo1Gb5KBGbicLoxhEX2458r4VrDgM_EYlHP1lpM2dnQswKwuAx.LrpBdU1Cm4QGOFlKnDOlgGHdOF.sFtHpbZyV8Vh7l1d9nmT3_Y5lVV8tqoSOzZgLpv5qXoW4YWDG32_4wweNXD4o.XWXfqpt3XrE_lJkH_AdPdx30q_nBr6X7BOqwgt8RNyzKCr9ITpdzqoFk_MaRmPFrzLZ5qa0I2q8YmxEXRBw0BzkZXB6n2I8Wz7zyNYobNjq5am1osGxS5sj48pFOJPP5Fnmc88Ln5PmBWNvQyEu_7vRk3KV.Y4D3F4i2bJ"};var cpo = document.createElement('script');cpo.src = '/cdn-cgi/challenge-platform/h/b/orchestrate/chl_page/v1?ray=8e9e5640ac1fcdea';window._cf_chl_opt.cOgUHash = location.hash === '' && location.href.indexOf('#') !== -1 ? '#' : location.hash;window._cf_chl_opt.cOgUQuery = location.search === '' && location.href.slice(0, location.href.length - window._cf_chl_opt.cOgUHash.length).indexOf('?') !== -1 ? '?' : location.search;if (window.history && window.history.replaceState) {var ogU = location.pathname + window._cf_chl_opt.cOgUQuery + window._cf_chl_opt.cOgUHash;history.replaceState(null, null, "\/doi\/10.1021\/bi0020067?__cf_chl_rt_tk=TI76maBnIZeuNGNEL.gNJHw.piPRsbF0ybmlIET8EQs-1732838630-1.0.1.1-yokRjDBP31cKXW_rQGcuWCxZ.Zj10SqHNdR6Wh0.ysM" + window._cf_chl_opt.cOgUHash);cpo.onload = function() {history.replaceState(null, null, ogU);}}document.getElementsByTagName('head')[0].appendChild(cpo);}());</script></body></html>