<?xml version="1.0" encoding="UTF-8"?> <article key="pdf/10008830" mdate="2018-03-03 00:00:00"> <author>Rezvan Najafi and Korosh Heydari and Massoud Saidijam</author> <title>miR200c as a Biomarker for 5FU Chemosensitivity in Colorectal Cancer</title> <pages>13 - 18</pages> <year>2018</year> <volume>12</volume> <number>2</number> <journal>International Journal of Medical and Health Sciences</journal> <ee>https://publications.waset.org/pdf/10008830</ee> <url>https://publications.waset.org/vol/134</url> <publisher>World Academy of Science, Engineering and Technology</publisher> <abstract>5FU is a chemotherapeutic agent that has been used in colorectal cancer (CRC) treatment. However, it is usually associated with the acquired resistance, which decreases the therapeutic effects of 5FU. miR200c is involved in chemotherapeutic drug resistance, but its mechanism is not fully understood. In this study, the effect of inhibition of miR200c in sensitivity of HCT116 CRC cells to 5FU was evaluated. HCT116 cells were transfected with LNAanti miR200c for 48 h. mRNA expression of miR200c was evaluated using quantitative real time PCR. The protein expression of phosphatase and tensin homolog (PTEN) and Ecadherin were analyzed by western blotting. Annexin V and propidium iodide staining assay were applied for apoptosis detection. The caspase3 activation was evaluated by an enzymatic assay. The results showed LNAantimiR200c inhibited the expression of PTEN and Ecadherin protein, apoptosis and activation of caspase 3 compared with control cells. In conclusion, these results suggest that miR200c as a prognostic marker can overcome to 5FU chemoresistance in CRC. </abstract> <index>Open Science Index 134, 2018</index> </article>