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<!DOCTYPE html> <html lang="en"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-width, initial-scale=1"> <meta http-equiv="X-UA-Compatible" content="IE=edge" /> <meta name="robots" content="index,follow" /> <meta name="googlebot" content="index,follow" /> <link rel="canonical" href="https://www.imedpub.com//abstract/mechanical-stimulation-of-piezo1-induces-runx2-gene-expression-through-the-aktgsk3-catenin-pathway-in-mc3t3e1-cells-36875.html"> <link rel="alternate" href="https://www.imedpub.com/abstract/mechanical-stimulation-of-piezo1-induces-runx2-gene-expression-through-the-aktgsk3-catenin-pathway-in-mc3t3e1-cells-36875.html" hreflang="en-us"/> <link rel="shortcut icon" href="https://www.imedpub.com/images/favicon.ico"/>  <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/5.11.2/css/all.min.css" /> <link href='https://fonts.googleapis.com/css?family=Open+Sans' rel='stylesheet' type='text/css' /> <link rel="stylesheet" href="/assets/css/imedpub_com.css" /> <link rel="stylesheet" href="/assets/css/global.css" /> <link href="https://www.imedpub.com/css/imed_external_style.css" rel="stylesheet" type="text/css" /> <link href="https://www.imedpub.com/css/styles.css" rel="stylesheet" type="text/css" /> <link href="https://www.imedpub.com/assets/css/author.css" rel="stylesheet" type="text/css" /> <link rel="stylesheet" type="text/css" href="https://www.imedpub.com/css/jquery.coolautosuggest.css" /> <meta name="google-site-verification" content="Fvj81ZSJ79zLnj_qZffRw_IKsNy7-JCt6kPdcx-H_zM" />  <script async src="https://platform.twitter.com/widgets.js" charset="utf-8"></script> <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script> <script src="https://www.imedpub.com/js/jquery.coolautosuggest.js"></script> <script src="https://www.imedpub.com/js/jquery.coolfieldset.js"></script> <script async src="https://pagead2.googlesyndication.com/pagead/js/adsbygoogle.js?client=ca-pub-8046643215361735" crossorigin="anonymous"></script> <script async src="https://www.googletagmanager.com/gtag/js?id=G-6X3EXTTCQV"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-6X3EXTTCQV'); </script>  <script> !function(f,b,e,v,n,t,s){if(f.fbq)return;n=f.fbq=function(){n.callMethod? n.callMethod.apply(n,arguments):n.queue.push(arguments)};if(!f._fbq)f._fbq=n; n.push=n;n.loaded=!0;n.version='2.0';n.queue=[];t=b.createElement(e);t.async=!0; t.src=v;s=b.getElementsByTagName(e)[0];s.parentNode.insertBefore(t,s)}(window, document,'script','//connect.facebook.net/en_US/fbevents.js'); fbq('init', '297919997051754'); fbq('track', "PageView"); </script> <title>Mechanical stimulation of PIEZO1 induces Runx-2 gene expression through the Akt/GSK-3 catenin pathway in MC3T3-E1 cells | Abstract</title> <meta name="keywords" content="Jidong Song and Xiaoqian Dang, Osteoporosis, Orthopedics, Surgery"/> <meta name="description" content="Mechanically-induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, t.. "/> <meta name="citation_publisher" content="iMedPub" /> <meta name="citation_journal_title" content="Journal of Surgery and Emergency Medicine"> <meta name="citation_title" content="Mechanical stimulation of PIEZO1 induces Runx-2 gene expression through the Akt/GSK-3 catenin pathway in MC3T3-E1 cells"> <meta name="citation_author" content="Jidong Song" /> <meta name="citation_author" content="Xiaoqian Dang" /> <meta name="citation_month" content="April"> <meta name="citation_year" content="2021"> <meta name="citation_volume" content="5"> <meta name="citation_issue" content="0"> <meta name="citation_publication_date" content="2021/05/08" /> <meta name="citation_abstract" content="Mechanically-induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 functions as a new mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)-induced signaling cascades that control osteogenesis. We applied a physiological range of fluid shear stress to the osteoblasts using a horizontal shaking apparatus. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3-E1 cells. The fluid shear stress promoted osteoblastic proliferation and elicited the key osteoblastic gene Runx-2 overexpression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK-3β/β-catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically-induced activation of the AKT/GSK-3β/β-catenin pathway. Therefore, the results demonstrated that MC3T3-E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx-2 gene overexpression, partly through the AKT/GSK-3β/β-catenin pathway."> <meta name="citation_pdf_url" content="https://www.imedpub.com/articles/mechanical-stimulation-of-piezo1-induces-runx2-gene-expression-through-the-aktgsk3catenin-pathway-in-mc3t3e1-cells.pdf"> <meta name="citation_abstract_html_url" content="https://www.imedpub.com/abstract/mechanical-stimulation-of-piezo1-induces-runx2-gene-expression-through-the-aktgsk3-catenin-pathway-in-mc3t3e1-cells-36875.html"> </head> <body> <div class="container-fluid"> <div class="container container_bg">  <header class="row justify-content-between align-items-center px-3"> <div class="col-12 col-sm">  <h1><a class="text-danger" href="https://www.imedpub.com/surgery-and-emergency-medicine/" title="Journal of Surgery and Emergency Medicine">Journal of Surgery and Emergency 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</ul>   </div>  </nav>   <div class="row clearfix mrgn_top">  <div class="col-lg-9 col-md-9"> <div class="wlcme_imdpub"> <h1>Abstract</h1> <div> <h2 style="font-size:18px;"> Mechanical stimulation of PIEZO1 induces Runx-2 gene expression through the Akt/GSK-3 catenin pathway in MC3T3-E1 cells </h2> <div class="clr"></div> <p>Mechanically-induced biological responses in bone cells involve a complex biophysical process. Although various mechanosensors have been identified, the precise mechanotransduction pathway remains poorly understood. PIEZO1 functions as a new mechanically activated ion channel in bone cells. This study aimed to explore the involvement of PIEZO1 in mechanical loading (fluid shear stress)-induced signaling cascades that control osteogenesis. We applied a physiological range of fluid shear stress to the osteoblasts using a horizontal shaking apparatus. The results showed that fluid shear stress increased PIEZO1 expression in MC3T3-E1 cells. The fluid shear stress promoted osteoblastic proliferation and elicited the key osteoblastic gene Runx-2 overexpression; however, PIEZO1 silencing using small interference RNA blocked these effects. The AKT/GSK-3β/β-catenin pathway was activated in this process. PIEZO1 silencing impaired mechanically-induced activation of the AKT/GSK-3β/β-catenin pathway. Therefore, the results demonstrated that MC3T3-E1 osteoblasts required PIEZO1 to adapt to the external mechanical fluid shear stress, thereby inducing osteoblastic Runx-2 gene overexpression, partly through the AKT/GSK-3β/β-catenin pathway.</p><br /> <strong>Author(s):</strong> Jidong Song and Xiaoqian Dang<br /> <a href="https://www.imedpub.com/articles/mechanical-stimulation-of-piezo1-induces-runx2-gene-expression-through-the-aktgsk3catenin-pathway-in-mc3t3e1-cells.pdf" title="Mechanical stimulation of PIEZO1 induces Runx-2 gene expression through the Akt/GSK-3 catenin pathway in MC3T3-E1 cells"></a><br /> <a href="https://www.imedpub.com/abstract/mechanical-stimulation-of-piezo1-induces-runx2-gene-expression-through-the-aktgsk3-catenin-pathway-in-mc3t3e1-cells-36875.html" title="Abstract">Abstract</a> | <a href="https://www.imedpub.com/articles/mechanical-stimulation-of-piezo1-induces-runx2-gene-expression-through-the-aktgsk3catenin-pathway-in-mc3t3e1-cells.pdf" 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