Cell Therapy Assays | Bio-Techne

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class="field_block:paragraph:h1_2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/immune-profiling-teaser-fore-green-480x307.png" alt="green immune cell sending out cytokines" fetchpriority="high" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/applications">Applications</a> </li> <li> <a href="/applications/immunoassays">Immunoassay Workflow Solutions</a> </li> <li> Assays For Immune Cell Therapy Development and Quality Control </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Successful process development minimizes cost while enhancing the efficiency and quality of <strong><a href="/research-areas/immune-cell-therapy/t-cell-manufacturing">T Cell and NK Cell therapies<sup>1</sup></a></strong>. R&D Systems' immunoassay solutions expedite immune cell therapy development and manufacturing workflows. Choose from our selection of reliable cell therapy assays—from the most flexible <a href="/reagents/luminex-assays"><strong>R&D Systems™ Luminex® assays*</strong></a>, industry-leading <a href="/reagents/elisa-kits/quantikine"><strong>Quantikine™ ELISA kits</strong></a>, or Simple Plex™ Assays on the <a href="/instruments/simple-plex"><strong>Ella</strong>™</a> instrument, a rapid and hands-free precision biomarker detection instrument.</p> <p> </p> <h2>Table of Contents</h2> <p><a name="top"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--clickable-icons-container paragraph--view-mode--default"> <div class="container"> <div class="row"> <div class="col p-0 "> <h2></h2> </div> </div> <div class="row"> <div class="col "> </div> </div> <div class="row"> <div class="row"> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#quantify-gmp" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/cell-culture.png' alt='Icon representing cell culture and activation'></div> </article> </div> <p><strong>Quantify GMP and Secreted Proteins</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#t-cell-activation" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/research-areas/t-cells-icon-2.png' alt='T Cell Icon'></div> </article> </div> <p><strong>Assess T Cell Activation</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#viral-titer-assays" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/cell-engineering.png' alt='Icon representing cell engineering'></div> </article> </div> <p><strong>Viral Titer Assays</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#qc-release" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/icon-qc-release-testing.png' alt='QC Release Testing'></div> </article> </div> <p><strong>QC Release Testing</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#impurity-testing" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/cell-harvesting.png' alt='Icon representing cell harvesting and quality control'></div> </article> </div> <p><strong>Process Impurity Testing</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#in-vivo" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/in-vivo-monitoring.png' alt='icons representing in vivo monitoring'></div> </article> </div> <p><strong>In Vivo Monitoring</strong></p> </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Quantify GMP and Secreted Proteins</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="quantify-gmp"></a>Immunoassays are required to confirm the precise addition and clearance of GMP growth factors during the cell culture phases of the immune cell therapy workflow.  Excessive growth factor exposure can cause T cell overactivation and exhaustion. <strong><a href="/reagents/elisa-kits/quantikine">Quantikine ELISA kits</a>,</strong> automated Simple Plex assays on the <strong><a href="/instruments/simple-plex">Ella instrument</a>,</strong> and <strong><a href="/reagents/luminex-assays">Luminex assays</a></strong> can be used to quantify GMP and secreted cytokines during the cell culture, activation, and expansion stages of the workflow.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/2-gmp-qkit.png" alt="GMP human IL-2, IL-7, and IL-15 are interpolated on the Quantikine ELISA standard curve." loading="lazy" > </div> <div><p>GMP-grade human IL-2, IL-7, and IL-15 (green circles) were interpolated with the appropriate Quantikine High Sensitivity ELISA Kit standard curve (blue circles) using 4PL logistic regression analysis.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/2-gmp-simple-plex.png" alt="GMP human IL-2, IL-7 and IL-15 are interpolated on the Ella automated ELISA standard curve." loading="lazy" > </div> <div><p>GMP-grade human IL-2, IL-7, and IL-15 (green circles) were interpolated with the appropriate standard curve (blue circles) using 4PL logistic regression analysis. Automated Simple Plex assays on the <strong><a href="/instruments/simple-plex">Ella instrument </a></strong>return data in 90 minutes or less.</p></div> <a href="#table" class="btn btn-primary" type="link"> Purchase Preclinical & GMP Proteins & Immunoassays </a> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p> <p><a name="t-cell-activation"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Assess T Cell Activation</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Immune cell therapy efficacy is influenced by a number of factors including T cell activation, which must be evaluated during the manufacturing workflow. Key T cell activation markers include Granzyme B, IFN-gamma, IL-2, Perforin, and TNF- alpha. Automated T cell activation marker assays on the <strong><a href="/instruments/simple-plex">Ella instrument</a> </strong>include a built-in factory standard curve, up to 4 logs of dynamic range, and 21 CFR Part 11 compliance for cell therapy manufacturing processes. These assays are vertically integrated with R&D Systems antibodies to deliver reproducible data you can trust. <strong><a href="/reagents/elisa-kits/quantikine">Quantikine ELISA kits</a></strong>, the most referenced ELISA on the market are also available to profile the T cell response.</p> <p><strong><a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/cell-kill-appnote.pdf" target="_blank">Download the Cell Killing Application Note</a></strong></p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/2-t-cell-activation-markers-data-3.png" alt="T cell activation markers are elevated when CD19 CAR T cells are co-cultured with CD19+ Nalm-6 cancer cells." loading="lazy" > </div> <div><p><br> T cell activation markers are robustly elevated when CD19 CAR T cells are co-cultured with CD19+ Nalm-6 cancer cells, but not CD19- K562 cells. T cell activation markers Granzyme B, TNF-α, IFN-γ, and IL-2 were assayed in parallel using a 32x4 Multianalyte cartridge on the <strong><a href="/instruments/simple-plex">Ella instrument</a></strong>. Bar graphs: mean ± SD (n=3).</p></div> <a href="#table" class="btn btn-primary" type="link"> Quantify Secreted T Cell Activation Markers </a> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p> <p><a name="viral-titer-assays"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Automated Viral Titer Assays</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Viral vectors are a popular tool for gene transfer in immune cell therapy workflows. Control of critical quality attributes (CQAs) such as viral titer is vital for maintaining therapeutic consistency. By automating the AAV and Lentiviral titer quantification process, Simple Plex viral titer assays on Ella decrease the time to result and reduce user error thereby enhancing team productivity and product consistency. Our viral titer assays are developed in collaboration with AAV industry leader <strong><a href="https://us.progen.com/AAV/" target="_blank">PROGEN</a></strong>. </p> <p><strong>Purchase Assays:  <a href="/p/simple-plex/simple-plex-aav1-cartridge_spckb-ot-007969" target="_blank">AAV1 Viral Titer Assay</a></strong> |<a href="/p/simple-plex/simple-plex-aav2-cartridge_spckb-ot-005905" target="_blank"> <strong>AAV2 Viral Titer Assay</strong></a> | <strong><a href="/p/simple-plex/simple-plex-aav6-cartridge_spckb-ot-007971" target="_blank">AAV6 Viral Titer Assay</a></strong> | <strong><a href="/p/simple-plex/simple-plex-aav8-cartridge_spckb-ot-007970" target="_blank">AAV8 Viral Titer Assay</a></strong> | <strong><a href="/p/simple-plex/simple-plex-hiv-1-gag-p24-cartridge_spckb-ps-000981" target="_blank">HIV-1 p24 Viral Titer Assay</a></strong><br>  </p> <p> </p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/titrate-aav-1268.png" alt="Automated AAV (1,2,6,8) titration assays on the Ella platform are highly correlated with PROGEN ELISAs" loading="lazy" > </div> <div><p><br> Linear correlations of the Simple Plex AAV viral titer assays and PROGEN AAV ELISAs. The aggregated Simple Plex and PROGEN ELISA data were plotted and a linear fit was applied to the data for each AAV serotype. The Simple Plex AAV viral titer assays are correlated with the corresponding PROGEN ELISAs as follows: AAV1 R<sup>2</sup>=0.99, AAV2 R<sup>2</sup>=0.99, AAV6 R<sup>2</sup>=0.99, AAV8 R<sup>2</sup>=0.99. The data showed a nearly perfect correlation where the quantitative range of both viral titer assays overlap.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/lod-aav-1268.png" alt="Automated AAV (1,2,6,9) titration assays on the Ella platform have excellent linearity of dilution (LOD)." loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p><br> The linearity of dilution was evaluated using in-process samples across multiple matrices. Each in-process sample showed good dilutional linearity at all 4 dilutions tested, with 80-120% percent recoveries compared to the minimum required dilution (MRD).</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--individual-button mb-4 paragraph--view-mode--default w-100"> <div class="row"> <div class="col"> <a href="/resources/instrument-applications/simple-plex-viral-titer-assays" class="btn btn-primary float-left" type="link" > Learn More About Simple Plex Viral Titer Assays </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p> <p><a name="qc-release"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>QC Release Testing with Ella™ IFN-gamma Assays</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Potency assays play a critical role in QC release testing—helping to propel your therapeutic across the finish line. That’s where efficient, high-throughput Ella assays come into play, offering the ideal solution for precise, reliable quality control testing. Ella gives you the critical information you need faster, with superior sensitivity and reproducibility that supports regulatory compliance.</p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/research-areas/immune-cell-therapy/ifn-gamma-assay-qc-release-testing-ella" class="btn btn-primary" type="link"> Learn More About Ella for QC Release Testing </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/immune-cell-culture-lp_Ella Teaser3.png" alt="Ella instrument" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p> <p><a name="impurity-testing"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Automated Process Impurity Testing: HEK 293 HCP | Endonuclease</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Human Embryonic Kidney (HEK) 293 cells are used for the production and scale-up of viral vectors. HEK host cell proteins (HCPs) must be cleared via in-process testing to avoid adverse host responses. Ella's automated <strong><a href="/p/simple-plex/simple-plex-hek-293-hcp-3g-cartridge_spckb-ot-007066">HEK HCP process residual assay</a></strong> is developed in partnership with <a href="https://www.cygnustechnologies.com/products/elisa-kits/host-cell-protein-elisa-kits/mammalian-hcp-elisa-kits.html?expression_system=682" target="_blank"><strong>Cygnus Technologies</strong></a>. This assay recognizes thousands of HCP species, satisfies the demands of timely data generation for maintaining efficient production, and overcomes the lengthy investigations and sample retests that often result from human error.</p> <p><strong>Purchase the <a href="/p/simple-plex/simple-plex-hek-293-hcp-3g-cartridge_spckb-ot-007066">HEK 293 HCP Assay</a></strong></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/correlate-hek-hcp.png" alt="The automated HEK HCP 3G assay on the Ella platform is highly correlated with the Cygnus Technologies ELISA." loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p><br> The Simple Plex HEK 293 HCP 3G assay is highly correlated (R²=0.9799) with the Cygnus HEK 293 HCP ELISA Kit, 3G Resupply 1.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/in-process-hek-hcp.png" alt="In process HEK HCP testing data from automated ELISAs on the Ella platform is very consistent with Cygnus Technology ELISAS" loading="lazy" > </div> <div><p>Detection of HEK HCPs during bioproduction with the Simple Plex HEK 293 HCP 3G assay and the Cygnus HEK 293 HCP ELISA Kit,3G Resupply 1 ELISA. Three industrial purification processes were profiled using the Cygnus ELISA (green), and the Simple Plex assay (blue). These processes were measured at multiple steps (X-axis) to show the elimination of HEK HCP from the product. Across each process, the Simple Plex assay demonstrates the same fold reduction in HEK HCP as the Cygnus ELISA although some differences in absolute sample values were observed.</p></div> <a href="/resources/instrument-applications/simple-plex-bioprocess-development" class="btn btn-primary" type="link"> Ella Expedites Immune Cell Therapy Process Development </a> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Rapid Endonuclease Quantification</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Endonuclease is useful for applications where host cell nucleic acid digestion is necessary. Such applications include viral vector preparations, vaccine products, and other biotherapeutics. Once the host-cell nucleic acid is digested, one must verify that residual endonuclease has been cleared from the final product. The Simple Plex Endonuclease assay is an easy-to-use immunoassay for detecting residual endonuclease impurities in bioprocess samples.</p> <p><strong><a href="/p/simple-plex/simple-plex-endonuclease-cartridge_spckc-ot-008612">Purchase the Simple Plex Endonuclease Assay</a></strong></p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/resources/literature/rapid-endonuclease-quantification" class="btn btn-primary" type="link"> Download the Endonuclease Application Note </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/lg-rapid-endoclease-an-mockup-500x650.png" alt="Rapid Endonuclease App Note" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p> <p><a name="in-vivo"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2><em>In Vivo</em> Monitoring</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h4><strong>R&D Systems Luminex Assays</strong></h4> <p>Cytokine release syndrome (CRS) is an adverse response to CAR T cell therapy. IFN-γ, IL-6, IL-10, and TNF-alpha are the core cytokines involved in CRS. R&D Systems Luminex High Performance Assay Panels are validated for use with human serum and plasma samples. Select from a premixed, user-mixed, or fixed panel, based on your needs.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/2-luminex-46-plex-cytokine-data.png" alt="Cytokines are elevated in stimulated but not unstimulated PBMC’s." loading="lazy" > </div> <div><p>The XL Cytokine Performance Panel detects the inflammatory response of CRS biomarkers. These graphs visualize the quantification of CRS analytes in Peripheral Blood Mononuclear Cell (PBMC) supernatants before and after stimulation with anti-CD3 epsilon and anti-CD28.</p></div> <a href="/reagents/luminex-assays/luminex-high-performance-assays/human-xl-cytokine-luminex-performance-assay" class="btn btn-primary" type="link"> View Validation Data and Purchase the Human XL Cytokine Performance Panel </a> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Quantify Interferon Gamma with Confidence</h2></div> <a href="https://www.rndsystems.com/quantify-ifn-gamma-confidence" > <button type="button" class="btn btn-primary"> Learn More at R&D Systems.com </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p> <p><a name="table"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Purchase Preclinical & GMP Proteins and Immunoassays</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><figure class="responsive-figure-table"><table class="table table-striped"> <tbody> <tr> <td><strong>Target</strong></td> <td><strong>Preclinical Protein</strong></td> <td><strong>GMP Protein</strong></td> <td><strong>Quantikine ELISA</strong></td> <td><strong>Simple Plex Assay</strong></td> <td><strong>Luminex Assay</strong></td> </tr> <tr> <td>Granzyme B</td> <td><a href="/p/proteins-enzymes/recombinant-human-granzyme-b-protein-cf_2906-se">Human</a></td> <td> </td> <td><a href="/p/elisa-kits/human-granzyme-b-quantikine-hs-elisa-kit_hsgzb0">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-granzyme-b-cartridge_spckb-ps-000496">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IFN-gamma</td> <td><a href="/t/ifn-gamma/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-ifn-gamma-gmp-protein-cf_285-gmp">yes</a></td> <td><a href="/t/ifn-gamma/elisa-kits">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-ifn-gamma-3rd-gen-cartridge_spckb-ps-002574">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-1 beta</td> <td><a href="/t/il-1-beta-il-1f2/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-il-1-beta-il-1f2-gmp-protein-cf_201-gmp">yes</a></td> <td><a href="/t/il-1-beta-il-1f2/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-1-beta-cartridge_spckb-ps-000216">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-2</td> <td><a href="/t/il-2/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-il-2-gmp-protein-cf_bt-002-gmp">yes</a></td> <td><a href="/t/il-2/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-2-cartridge_spckb-ps-000295">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-4</td> <td><a href="/t/il-4/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-il-4-gmp-protein-cf_204-gmp">yes</a></td> <td><a href="/t/il-4/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-4-cartridge-2nd-generation_spckb-ps-002800">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-6</td> <td><a href="/t/il-6/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-il-6-gmp-protein-cf_206-gmp">yes</a></td> <td><a href="/t/il-6/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-6-2nd-gen-assay-cartridge_spckb-ps-003028">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-7</td> <td><a href="/t/il-7/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-il-7-gmp-protein-cf_bt-007-gmp">yes</a></td> <td><a href="/p/elisa-kits/human-il-12-p70-duoset-elisa_dy1270">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-7-cartridge_spckb-ps-000506">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-8</td> <td><a href="/p/proteins-enzymes/recombinant-human-il-8-cxcl8-protein_208-il">Human</a></td> <td> </td> <td><a href="/t/il-8-cxcl8/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-8-cartridge_spckb-ps-000230">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-12</td> <td><a href="/t/il-12/proteins-enzymes?species=Human">Human</a></td> <td> </td> <td><a href="/p/elisa-kits/human-il-12-p70-duoset-elisa_dy1270">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-12-p70-cartridge_spckb-ps-000498">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-15</td> <td><a href="/t/il-15/proteins-enzymes?species=Human">Human</a></td> <td><a href="/t/il-15/proteins-enzymes">yes</a></td> <td><a href="/t/il-15/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-15-cartridge_spckb-ps-000500">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-18</td> <td><a href="/t/il-18-il-1f4/proteins-enzymes?species=Human">Human</a></td> <td> </td> <td><a href="/t/il-18-il-1f4/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-il-18-cartridge_spckb-ps-000501">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-21</td> <td><a href="/t/il-21/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-il-21-gmp-protein-cf_8879-gmp">yes</a></td> <td><a href="/t/il-21/elisa-kits?species=Human">yes</a></td> <td> </td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-23</td> <td><a href="/t/il-23/proteins-enzymes?species=Human">Human</a></td> <td> </td> <td><a href="/t/il-23/elisa-kits?species=Human">yes</a></td> <td> </td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>IL-27</td> <td><a href="/t/il-27/proteins-enzymes?species=Human">Human</a></td> <td> </td> <td><a href="/t/il-27/elisa-kits?species=Human">yes</a></td> <td> </td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> <tr> <td>Perforin</td> <td> </td> <td> </td> <td> </td> <td><a href="/p/simple-plex/simple-plex-human-perforin-cartridge_spckb-ps-006982">yes</a></td> <td> </td> </tr> <tr> <td>TGF-beta 1</td> <td><a href="/t/tgf-beta-1/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-tgf-beta-1-gmp-protein-cf_240-gmp">yes</a></td> <td><a href="/t/tgf-beta-1/elisa-kits?species=Human">yes</a></td> <td><a href="/t/tgf-beta-1/simple-plex?species=Human">yes</a></td> <td> </td> </tr> <tr> <td>TNF-alpha</td> <td><a href="/t/tnf-alpha/proteins-enzymes?species=Human">Human</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-gmp-protein-cf_210-gmp">yes</a></td> <td><a href="/t/tnf-alpha/elisa-kits?species=Human">yes</a></td> <td><a href="/p/simple-plex/simple-plex-human-tnf-alpha-2nd-gen-cartridge_spckb-ps-002803">yes</a></td> <td><a href="/luminex-assay-customization-tool">yes</a></td> </tr> </tbody> </table></figure> <p><strong>This list is not exhaustive. Don't see what you need? Search for your target using the search bar at the <a href="#top">top</a> of the page.</strong></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#top">Return To Top</a></p> <p><a name="bulk"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Bulk Immunoassay Services</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Did you know that Bio-Techne is vertically integrated? Vertical integration enables us to control our supply chain and enable a consistent cell and gene therapy development and manufacturing workflow. Bio-Techne is a partner that you can grow with.</p> <p>Need help with an immunoassay-specific project? Start a conversation with an Immunoassay Specialist.</p> <p><strong><a href="/services/custom-elisa-services" rel="noreferrer noopener">Bulk ELISA Assay Services </a></strong> | <strong><a href="/services/custom-luminex-services" rel="noreferrer noopener">Bulk Luminex Assay Services</a> </strong> | <strong><a href="/services/custom-simple-plex-services">Bulk Ella Services</a></strong></p></div> </div> </div> <div> <div id="accordion" class="paragraph mb-4 paragraph--type--references-container paragraph--view-mode--default"> <div class="card-header"> <a class="collapsed" data-toggle="collapse" data-target="#refPara-94601" role="button" aria-expanded="true" aria-controls="refPara-94601"> <h5 class="d-inline">References</h5> </a> </div> <div id="refPara-94601" class="paragraph mb-4 paragraph--type--references-container paragraph--view-mode--default collapse accordion" aria-controls="refPara-94601"> <ol class="py-3"> <li class="py-2"> <p class="paragraph paragraph--type--individual-reference paragraph--view-mode--default image-and-text-default"> <strong>Campbell, A</strong> <em> et al. </em> (2015) <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572896/" title="View Abstract" target="_blank" rel="noreferrer"> Concise Review: Process Development Considerations for Cell Therapy</a> Stem Cells Translational Medicine <strong>4</strong>:1155. PMID: 26315572. </p> </li> </ol> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><br> *Luminex<sup>®</sup> is a registered trademark of the Luminex Corporation - A DiaSorin Company</p> <p>  <a href="#top">Return To Top</a></p></div> </div> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> <div class="footer-logo col-xl-3 col-lg-auto d-flex"> <img class="brand-bar-footer-logo" src="/themes/custom/bio_techne_global/logo.svg" alt="Bio-techne logo"> </div> <div class="col-xl-9 col-lg-auto"> <p class="ml-xl-4 bt-px_groteskbold footer-tagline">Global Developer, Manufacturer, and Supplier of High-Quality Reagents, Analytical Instruments, and Precision Diagnostics.</p> </div> <div class="included-brands col"> <span class="font-weight-bold mr-3 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