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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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rel="preload" href="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/PG3011-PJT12698-COL122488-eT4-Web-Hdr-Child-Pg4-SuperFi-Global-1600x250-F.jpg"/><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/PG3011-PJT12698-COL122488-eT4-Web-Hdr-Child-Pg4-SuperFi-Global-1600x250-F.jpg" alt="" height="250px" width="1600px" class="well-background-image"/><div class="well-content"><div class="parsys"><div class="text parbase section"><div class="table-component-wrapper"><table style="width: 100%;" id="table-168542204"><tbody><tr><td style="vertical-align: middle; height: 250px;"><h1 style="color: #fff;">Platinum SuperFi II DNA Polymerase—A High Fidelity<br> DNA Polymerase</h1><p><a class="btn btn-primary" href="/sg/en/home/global/forms/platinum-superfi-samples-form.html">Request sample</a></p> </td></tr></tbody></table></div></div></div></div></div></div></div></div></div></div></div><div class="container page-header-hidden"><div class="row"><div class="span12"><div class="pageheading"></div></div></div></div><div class="container-wrap template-2-column"><div class="container"><div class="row"><script> var showHideText = { 'seeRelatedText':'See Navigation', 'hideRelatedText':'Hide Navigation' }; </script><div class="span3 sidebar" role="complementary"><div id="link-leftnav-toggle-container"><a class='link-leftnav-toggle' href='#'>See Navigation</a></div><div class="container-leftnav"><div class="railInheritParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="railInheritParsys iparsys parsys"><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> &nbsp <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes.html"><b>PCR Enzymes and Master Mixes</b></a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/pcr-enzymes-selection-tool.html">Enzyme selection tool</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Hot-start PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq</i> Hot-Start DNA polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html">AmpliTaq Gold DNA polymerase</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> High-fidelity PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/accuprime-dna-polymerases.html">AccuPrime <i>Taq</i> DNA Polymerase High Fidelity</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Long-range PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase </a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> GC-rich PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq</i> DNA Polymerase</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Multiplex PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase </a></li><li><a href="/order/catalog/product/4464270">Platinum Multiplex PCR Master Mix</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Direct PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html">Platinum Direct PCR Universal Master Mix</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Standard PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/invitrogen-taq-dna-polymerases.html"><i>Taq</i> DNA polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-dna-polymerases.html">AmpliTaq DNA polymerase</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Resources <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education.html">PCR education</a></li><li><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/tm-calculator.html">T<sub>m</sub> calculator</a></li><li><a href="/sg/en/home/brands/invitrogen/molecular-biology-technologies/pcr-video-library.html#pcr.html">PCR videos</a></li><li><a href="/sg/en/home/brands/invitrogen/molecular-biology-technologies/pcr-literature-resource-library.html?open=pcr#.html">PCR app notes</a></li><li><a href="/sg/en/home/brands/invitrogen/molecular-biology-technologies/pcr-webinars.html?open=pcr#.html">PCR webinars</a></li><li><a href="/sg/en/home/about-us/partnering-licensing/oem-commercial-supply/life-science-tools.html">OEM & customized PCR solutions</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Related products <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/oligonucleotides-primers-probes-genes/custom-dna-oligos.html">PCR primers</a></li><li><a href="/sg/en/home/life-science/oligonucleotides-primers-probes-genes/nucleotides.html">dNTPs</a></li><li><a href="/sg/en/home/life-science/pcr/thermal-cyclers-realtime-instruments.html">Thermal cyclers & real-time PCR instruments</a></li><li><a href="/sg/en/home/life-science/pcr/reverse-transcription.html">Reverse transcription</a></li><li><a href="/sg/en/home/life-science/pcr/real-time-pcr.html">Real-Time PCR (qPCR)</a></li><li><a href="/sg/en/home/life-science/pcr/digital-pcr.html">Digital PCR</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-plastics.html">PCR tubes, plates & accessories</a></li></ul></li></ul></div></div></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="text parbase section"><p>High fidelity DNA polymerases are PCR enzymes that amplify target sequences accurately. These enzymes achieve high fidelity due to their proofreading ability, which correct misincorporated nucleotides during amplification. Invitrogen Platinum SuperFi II DNA Polymerase is a hot-start, engineered DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x <i>Taq</i> fidelity and buffer specially formulated for primer annealing at 60°C, Platinum SuperFi II DNA Polymerase offers efficiency and simplicity in PCR applications requiring highest PCR accuracy, such as cloning, sequencing, and mutagenesis.</p></div><div class="regionalwrapper parbase section"></div><div class="heading section"><h2> Highlights of Platinum SuperFi II DNA Polymerase </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><b>Exceptional accuracy</b>—Higher than 300x <i>Taq</i> fidelity as determined by next-generation sequencing</li><li><b>Simplified workflow</b>—Buffer formulated for primer annealing at 60°C (no need for a Tm calculator)</li><li><b>Increased PCR success</b>—Robust amplification of GC-rich targets, DNA of suboptimal purity, and long sequences</li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><ul><li><b>Enabled automation</b>—High specificity and benchtop stability for 24 hours after reaction setup, enabled by Invitrogen Platinum hot-start technology</li><li><b>Reduced pipetting</b>—Master mix options available with or without direct gel-loading dyes</li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="brightcoveplayer section"><div data-video="1b1b2dc98eb3"><script type="text/javascript"> var loadEME = true; 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var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><p>Watch the video to learn about the fidelity of a DNA polymerase, methods for measuring enzyme fidelity, and benefits of using a high-fidelity DNA polymerase in your PCR</p></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="brightcoveplayer section"><div data-video="bd881cd281d5"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-bd881cd281d5', 10); </script></div><div class="video-container video-single"><div id="span-video-bd881cd281d5" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-bd881cd281d5" class="brightcove-container"><script>document.createElement('video')</script><video id="video-bd881cd281d5" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="4837868174001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "What is high-fidelity PCR?", "description": "Learn about fidelity of a DNA polymerase, methods for measuring enzyme fidelity, and benefits of using a high-fidelity DNA polymerase in your PCR.", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/d45b138e-2309-412c-9e36-f2e66ee0f552/69a89318-874f-4964-a6c8-fa51e44128b5/160x90/match/image.jpg"], "uploadDate": "2016-04-08T20:06:26.923Z", "duration": "PT2M10.005S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/4837868174001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=4837868174001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=4837868174001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B4"><div class="text parbase section"><p>Watch the benefits of Platinum SuperFi II DNA Polymerase.</p></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2> Benefits of high fidelity Platinum SuperFi II DNA Polymerase </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-d4b64599-dddf-43c9-b447-6c0980258384">>300x <i>Taq</i> fidelity</a></li><li><a data-toggle="tab" href="#container-3bda70b9-dc38-4140-8bec-08b56b456fcb">60°C primer annealing</a></li><li><a data-toggle="tab" href="#container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65">Direct gel loading</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-d4b64599-dddf-43c9-b447-6c0980258384" class="tab-pane active"><div class="container-d4b64599-dddf-43c9-b447-6c0980258384 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> High fidelity </h3></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase preserves DNA sequence accuracy with its extremely low error rate. Using <a href="/sg/en/home/life-science/sequencing/next-generation-sequencing/ngs-library-preparation-illumina-systems.html">next-generation sequencing</a>, the relative <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Fidelity">fidelity</a> of Platinum SuperFi II DNA polymerase was calculated to be >300x that of <i>Taq</i> DNA polymerase (<b>Figure 1</b>).</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-d4b64599-dddf-43c9-b447-6c0980258384tabtextimage_716e"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.medium.jpg/1732033044191.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.480.high.jpg/1732033044191.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.620.high.jpg/1732033044191.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.full.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.480.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.medium.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.620.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.low.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.480.medium.jpg/1732033044191.jpg" alt='Bar graph showing Platinum SuperFi II DNA polymerase has the highest fidelity compared to Taq polymerase' title='Fidelity of commercial DNA polymerases compared to Taq DNA polymerase'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-d4b64599-dddf-43c9-b447-6c0980258384tabtextimage_716e { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-d4b64599-dddf-43c9-b447-6c0980258384tabtextimage_716e img { width: 100%; max-width: 695px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b><a id="figure1"></a>Figure 1. Fidelity comparison across commercially available enzymes relative to <i>Taq</i> enzyme.</b> A 3.9 kb sequence was amplified by PCR using different DNA polymerases, and the resulting PCR amplicons were then fragmented with a MuA transposase. Unique molecular identifiers (UMI), which consist of 12 random nucleotides, were introduced during fragmentation to individually tag each product. After next-generation sequencing, reads were aligned to the correct sequence, grouped by UMI families, and errors were called. Errors were identified only if they were present in all reads in the UMI family; otherwise, they were discarded as sequencing errors. The polymerase fidelities were normalized to the <i>Taq</i> DNA polymerase. </small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-3bda70b9-dc38-4140-8bec-08b56b456fcb" class="tab-pane"><div class="container-3bda70b9-dc38-4140-8bec-08b56b456fcb tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> 60°C primer annealing </h3></div><div class="text parbase section"><p>The innovative buffer formulation of Platinum SuperFi II buffer enables annealing of primers at 60°C regardless of their sequences (<b>Figure 2</b>). The buffer also allows successful amplification when calculated T<sub>m</sub>s are used in the annealing step (data not shown). This universal annealing feature allows co-cycling assays (<b>Figure 3 and 4</b>).</p></div><div class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_b374"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.full.high.jpg/1732033044345.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.full.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.320.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.480.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.320.medium.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.620.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.320.low.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.480.medium.jpg/1732033044345.jpg" alt='Gel image with clear bands' title='PCR products generated under universal annealing and calculated Tm'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_b374 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_b374 img { width: 100%; max-width: 460px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 2.</b><b>Platinum SuperFi II DNA Polymerase produces PCR products with high specificity and yield following the universal annealing temperature at 60°C.</b> Primer sets of varying annealing temperature were used to amplify 12 targets from 50 ng of human genomic DNA at 60<b>°</b>C annealing temperature. The molecular weight marker is <a href="/order/catalog/product/10488085" adhocenable="false">Invitrogen TrackIt 1 Kb Plus DNA Ladder</a>. The annealing temperatures stated were calculated using the <a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/tm-calculator.html" adhocenable="false">T<sub>m</sub> calculator</a> for Platinum SuperFi DNA Polymerase.</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_f40e"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.medium.png/1732033044406.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.480.high.png/1732033044406.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.620.high.png/1732033044406.png' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.full.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.480.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.medium.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.620.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.low.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.480.medium.png/1732033044406.png" alt='Line diagram shows that three targets can be amplified simultaneously' title='Illustration of co-cyling with universal PCR protocol'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_f40e { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_f40e img { width: 100%; max-width: 652px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 3. Time saving and assay co-cycling enabled by universal PCR protocol.</b> PCR assays using conventional PCR reagents require specific protocols for amplification of each DNA fragment because of the different primer annealing temperatures and extension steps; therefore, multiple targets often cannot be amplified together in the same PCR run. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using one protocol with a universal primer annealing temperature and the extension time selected for the longest fragment to be amplified (<b>Figure 4</b>).</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_82af"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.medium.jpg/1732033044464.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.480.high.jpg/1732033044464.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.620.high.jpg/1732033044464.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.full.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.480.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.medium.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.620.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.low.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.480.medium.jpg/1732033044464.jpg" alt='Gel image with bands of short and long amplicons' title='Short and long amplicons cycled in the same reaction'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_82af { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_82af img { width: 100%; max-width: 652px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 4. Platinum SuperFi II DNA Polymerase enables cycling of shorter and longer amplicons together.</b>  0.7 kb, 2.0 kb, 4.8 kb, and 14 kb fragments were amplified from 100 ng of human genomic DNA using the same protocol for all four targets: 98°C denaturation for 10 sec, 60°C annealing for 10 sec, 72°C extension for 7 min. The extension time was based on length of the longest target.</small></p></div></div><div class="clearfix"></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Learn about the benefits of universal annealing</a></p></div></div><div class="end"></div></div></div><div id="container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65" class="tab-pane"><div class="container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Direct gel loading </h3></div><div class="text parbase section"><p><a href="/order/catalog/product/12369010">Platinum SuperFi II Green PCR Master Mix</a> offers the convenience of direct gel loading of PCR products, eliminating tedious steps of dye addition to PCR samples, and helping reduce pipetting errors. The green buffer (<b>Figure 5</b>) is compatible with downstream applications including <a href="/sg/en/home/life-science/sequencing/sanger-sequencing.html">DNA sequencing</a>, <a href="/sg/en/home/life-science/cloning/restriction-enzyme-digestion-and-ligation/restriction-enzyme-cloning.html">ligation, and restriction digestion</a>.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-a3a05d1b-f0a5-41bc-9281-28ee52e02b65tabtextimage_52fa"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.high.jpg/1732033044577.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.480.high.jpg/1732033044577.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.full.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.480.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.medium.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.620.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.low.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.480.medium.jpg/1732033044577.jpg" alt='Schematic shows workflow sequence from tube of green mastermix and a target DNA to thermal cycler to colored electrophoretic bands' title='Direct gel loading workflow'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-a3a05d1b-f0a5-41bc-9281-28ee52e02b65tabtextimage_52fa { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-a3a05d1b-f0a5-41bc-9281-28ee52e02b65tabtextimage_52fa img { width: 100%; max-width: 564px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 5. The green master mix format for loading PCR products directly to a gel for analysis.</b> The master mix of Platinum SuperFi II DNA Polymerase is available in a green buffer format that contains a density reagent and two tracking dyes. DNA migration is easily tracked with two dyes (blue and yellow) that are readily visible during electrophoresis (the lanes for 5 and 15 min in the figure to the right).</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer" href="#collapse1"><span class="caret"></span><strong> >300x <i>Taq</i> fidelity </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-d4b64599-dddf-43c9-b447-6c0980258384"><div class="container-d4b64599-dddf-43c9-b447-6c0980258384 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> High fidelity </h3></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase preserves DNA sequence accuracy with its extremely low error rate. Using <a href="/sg/en/home/life-science/sequencing/next-generation-sequencing/ngs-library-preparation-illumina-systems.html">next-generation sequencing</a>, the relative <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Fidelity">fidelity</a> of Platinum SuperFi II DNA polymerase was calculated to be >300x that of <i>Taq</i> DNA polymerase (<b>Figure 1</b>).</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-d4b64599-dddf-43c9-b447-6c0980258384tabtextimage_716e"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.medium.jpg/1732033044191.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.480.high.jpg/1732033044191.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.620.high.jpg/1732033044191.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.full.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.480.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.medium.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.620.high.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.320.low.jpg/1732033044191.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-d4b64599-dddf-43c9-b447-6c0980258384/tab/textimage_716e/image.img.480.medium.jpg/1732033044191.jpg" alt='Bar graph showing Platinum SuperFi II DNA polymerase has the highest fidelity compared to Taq polymerase' title='Fidelity of commercial DNA polymerases compared to Taq DNA polymerase'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-d4b64599-dddf-43c9-b447-6c0980258384tabtextimage_716e { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-d4b64599-dddf-43c9-b447-6c0980258384tabtextimage_716e img { width: 100%; max-width: 695px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b><a id="figure1"></a>Figure 1. Fidelity comparison across commercially available enzymes relative to <i>Taq</i> enzyme.</b> A 3.9 kb sequence was amplified by PCR using different DNA polymerases, and the resulting PCR amplicons were then fragmented with a MuA transposase. Unique molecular identifiers (UMI), which consist of 12 random nucleotides, were introduced during fragmentation to individually tag each product. After next-generation sequencing, reads were aligned to the correct sequence, grouped by UMI families, and errors were called. Errors were identified only if they were present in all reads in the UMI family; otherwise, they were discarded as sequencing errors. The polymerase fidelities were normalized to the <i>Taq</i> DNA polymerase. </small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer" href="#collapse2"><span class="caret"></span><strong> 60°C primer annealing </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-3bda70b9-dc38-4140-8bec-08b56b456fcb"><div class="container-3bda70b9-dc38-4140-8bec-08b56b456fcb tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> 60°C primer annealing </h3></div><div class="text parbase section"><p>The innovative buffer formulation of Platinum SuperFi II buffer enables annealing of primers at 60°C regardless of their sequences (<b>Figure 2</b>). The buffer also allows successful amplification when calculated T<sub>m</sub>s are used in the annealing step (data not shown). This universal annealing feature allows co-cycling assays (<b>Figure 3 and 4</b>).</p></div><div class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_b374"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.full.high.jpg/1732033044345.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.full.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.320.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.480.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.320.medium.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.620.high.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.320.low.jpg/1732033044345.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_b374/image.img.480.medium.jpg/1732033044345.jpg" alt='Gel image with clear bands' title='PCR products generated under universal annealing and calculated Tm'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_b374 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_b374 img { width: 100%; max-width: 460px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 2.</b><b>Platinum SuperFi II DNA Polymerase produces PCR products with high specificity and yield following the universal annealing temperature at 60°C.</b> Primer sets of varying annealing temperature were used to amplify 12 targets from 50 ng of human genomic DNA at 60<b>°</b>C annealing temperature. The molecular weight marker is <a href="/order/catalog/product/10488085" adhocenable="false">Invitrogen TrackIt 1 Kb Plus DNA Ladder</a>. The annealing temperatures stated were calculated using the <a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/tm-calculator.html" adhocenable="false">T<sub>m</sub> calculator</a> for Platinum SuperFi DNA Polymerase.</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_f40e"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.medium.png/1732033044406.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.480.high.png/1732033044406.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.620.high.png/1732033044406.png' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.full.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.480.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.medium.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.620.high.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.320.low.png/1732033044406.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_f40e/image.img.480.medium.png/1732033044406.png" alt='Line diagram shows that three targets can be amplified simultaneously' title='Illustration of co-cyling with universal PCR protocol'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_f40e { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_f40e img { width: 100%; max-width: 652px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 3. Time saving and assay co-cycling enabled by universal PCR protocol.</b> PCR assays using conventional PCR reagents require specific protocols for amplification of each DNA fragment because of the different primer annealing temperatures and extension steps; therefore, multiple targets often cannot be amplified together in the same PCR run. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using one protocol with a universal primer annealing temperature and the extension time selected for the longest fragment to be amplified (<b>Figure 4</b>).</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_82af"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.medium.jpg/1732033044464.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.480.high.jpg/1732033044464.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.620.high.jpg/1732033044464.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.full.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.480.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.medium.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.620.high.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.320.low.jpg/1732033044464.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-3bda70b9-dc38-4140-8bec-08b56b456fcb/tab/textimage_82af/image.img.480.medium.jpg/1732033044464.jpg" alt='Gel image with bands of short and long amplicons' title='Short and long amplicons cycled in the same reaction'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_82af { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-3bda70b9-dc38-4140-8bec-08b56b456fcbtabtextimage_82af img { width: 100%; max-width: 652px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 4. Platinum SuperFi II DNA Polymerase enables cycling of shorter and longer amplicons together.</b>  0.7 kb, 2.0 kb, 4.8 kb, and 14 kb fragments were amplified from 100 ng of human genomic DNA using the same protocol for all four targets: 98°C denaturation for 10 sec, 60°C annealing for 10 sec, 72°C extension for 7 min. The extension time was based on length of the longest target.</small></p></div></div><div class="clearfix"></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Learn about the benefits of universal annealing</a></p></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer" href="#collapse3"><span class="caret"></span><strong> Direct gel loading </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65"><div class="container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Direct gel loading </h3></div><div class="text parbase section"><p><a href="/order/catalog/product/12369010">Platinum SuperFi II Green PCR Master Mix</a> offers the convenience of direct gel loading of PCR products, eliminating tedious steps of dye addition to PCR samples, and helping reduce pipetting errors. The green buffer (<b>Figure 5</b>) is compatible with downstream applications including <a href="/sg/en/home/life-science/sequencing/sanger-sequencing.html">DNA sequencing</a>, <a href="/sg/en/home/life-science/cloning/restriction-enzyme-digestion-and-ligation/restriction-enzyme-cloning.html">ligation, and restriction digestion</a>.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-a3a05d1b-f0a5-41bc-9281-28ee52e02b65tabtextimage_52fa"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.high.jpg/1732033044577.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.480.high.jpg/1732033044577.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.full.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.480.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.medium.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.620.high.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.320.low.jpg/1732033044577.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer/container-a3a05d1b-f0a5-41bc-9281-28ee52e02b65/tab/textimage_52fa/image.img.480.medium.jpg/1732033044577.jpg" alt='Schematic shows workflow sequence from tube of green mastermix and a target DNA to thermal cycler to colored electrophoretic bands' title='Direct gel loading workflow'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-a3a05d1b-f0a5-41bc-9281-28ee52e02b65tabtextimage_52fa { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainercontainer-a3a05d1b-f0a5-41bc-9281-28ee52e02b65tabtextimage_52fa img { width: 100%; max-width: 564px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 5. The green master mix format for loading PCR products directly to a gel for analysis.</b> The master mix of Platinum SuperFi II DNA Polymerase is available in a green buffer format that contains a density reagent and two tracking dyes. DNA migration is easily tracked with two dyes (blue and yellow) that are readily visible during electrophoresis (the lanes for 5 and 15 min in the figure to the right).</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2 id="data"><br>Benchmarking data comparing high fidelity polymerases </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-dba95534229571">Yield and specificity</a></li><li><a data-toggle="tab" href="#container-dba95534229572">High sensitivity </a></li><li><a data-toggle="tab" href="#container-dba95534229573">Inhibitor tolerance </a></li><li><a data-toggle="tab" href="#container-dba95534229574">Benchtop stability </a></li></ul><div class="tab-content" id="myTabContent"><div id="container-dba95534229571" class="tab-pane active"><div class="container-dba95534229571 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> High yield & specificity </div></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase amplifies a broad range of target lengths with high specificity and yield due to robustness of the enzyme and superior <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Specificity">hot-start technology</a>. The antibody-based Platinum hot-start technology inhibits enzyme activity until the initial PCR denaturation step, preventing nonspecific amplification and primer degradation while allowing greater yield of the target amplicons.</p></div><div class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div class="image parbase section"><div id="cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.low.png/1732033044805.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.medium.png/1732033044805.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.480.medium.png/1732033044805.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.620.high.png/1732033044805.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.full.high.png/1732033044805.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.full.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.480.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.medium.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.620.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.low.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.480.medium.png/1732033044805.png" alt='Composite of four gel images. Each gel has five lanes of samples' title='Comparison of performance of DNA polymerases in amplifying DNA targets of various lengths'></picture><style> #cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4 img{ width:100%; max-width: 1459px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4 .image-contain" + "," + "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"></div></div><div style="clear:both"></div><div class="text parbase section"><p><small><b>Figure 6. Versatility across broad range of amplicon lengths. </b>Platinum SuperFi II DNA Polymerase (far left panel) provides high specificity and yield across range of DNA fragments from 0.3 kb to 14 kb amplified from 100 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases: <b>A</b>: Merck KOD™ Hot Start, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: PrimeSTAR GXL. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="end"></div></div></div><div id="container-dba95534229572" class="tab-pane"><div class="container-dba95534229572 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> High sensitivity </div></div><div class="text parbase section"><p>The high sensitivity of Platinum SuperFi II DNA Polymerase enables detection of low-abundance DNA templates. High sensitivity is advantageous in experiments where there is a limited amount of starting material, or the target DNA is in low concentration in the sample.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229572tabtextimage_cee4"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.low.png/1732033044932.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.medium.png/1732033044932.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.480.medium.png/1732033044932.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.620.high.png/1732033044932.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.full.high.png/1732033044932.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.full.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.480.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.medium.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.620.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.low.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.480.medium.png/1732033044932.png" alt='Composite of four gel images; each gel has five lanes of samples' title='Comparison of performance of DNA polymerases in amplifying low abundance DNA templates'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229572tabtextimage_cee4 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229572tabtextimage_cee4 img { width: 100%; max-width: 1779px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 7. High sensitivity and reliable amplification from low amounts of input DNA.</b> Platinum SuperFi II DNA Polymerase (far left panel) shows reliable amplification of a 2 kb fragment from 0.4 ng, 2 ng, 10 ng, 50 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane.). The same target was amplified with competitor DNA polymerases: <b>A</b>: PfuUltra™ II Fusion Hot Start, <b>B</b>: HotStar™ HiFidelity DNA Polymerase Kit, <b>C</b>: Expand HiFiPLUS Enzyme Blend. The estimated copy number is ~100 copies per 0.4 ng of human genomic DNA. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-dba95534229573" class="tab-pane"><div class="container-dba95534229573 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> Inhibitor tolerance </div></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase is engineered with a DNA-binding domain resulting in <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Processivity">high processivity</a> and increased tolerance to common PCR inhibitors (<b>Figure 8</b>) such as, hemin, humic acid, and bile salt. </p></div><div class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div class="image parbase section"><div id="cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.low.png/1732033045077.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.medium.png/1732033045077.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.480.medium.png/1732033045077.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.620.high.png/1732033045077.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.full.high.png/1732033045077.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.full.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.480.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.medium.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.620.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.low.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.480.medium.png/1732033045077.png" alt='Composite of five gel images; each gel has four lanes of samples' title='Comparison of performance of DNA polymerases in the presence of common inhibitors'></picture><style> #cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9 img{ width:100%; max-width: 1614px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9 .image-contain" + "," + "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"></div></div><div style="clear:both"></div><div class="text parbase section"><p><small><b>Figure 8. Platinum SuperFi II DNA Polymerase shows high tolerance to common PCR inhibitors. </b>A 2 kb human genomic DNA fragment was amplified from 50 ng of human genomic DNA using Platinum SuperFi II DNA Polymerase or competitor high-fidelity DNA polymerases: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: PrimeSTAR GXL, <b>C</b>: Merck KOD™ Hot Start, and <b>D</b>: KAPA HiFi HotStart PCR Kit in reaction mixtures containing <b>1</b>: no inhibitor, <b>2</b>: humic acid (4 µg/mL), <b>3</b>: hemin (20 µM), and <b>4</b>: bile salt (1 mg/mL). The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="end"></div></div></div><div id="container-dba95534229574" class="tab-pane"><div class="container-dba95534229574 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> Benchtop stability </div></div><div class="text parbase section"><p>Extended stability of the Platinum SuperFi II DNA Polymerase enzyme at room temperature (Figure 9) enables high-throughput applications. Its superior Platinum <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Specificity">hot-start technology</a> enables benchtop stability and amplification with high specificity.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229574tabtextimage_7d61"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.full.high.png/1732033045193.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.full.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.320.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.480.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.320.medium.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.620.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.320.low.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.480.medium.png/1732033045193.png" alt='Gel image with 8 lanes' title='Comparison of performance of DNA polymerases after reactions were left for 24 hours at room temperature'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229574tabtextimage_7d61 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229574tabtextimage_7d61 img { width: 100%; max-width: 465px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 9. Assembled reactions with Platinum SuperFi II DNA Polymerase are stable room temperature.</b> A 0.5 kb fragment was amplified form 50 ng of human genomic DNA. PCR reactions were set up and left at room temperature for 0 hr and 24 hr before loading to the <a href="/sg/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/proflex-pcr-system.html">Applied Biosystems ProFlex thermal cycler</a>. Even after 24 hr of room-temperature setup, Platinum SuperFi II DNA Polymerase (<b>lane P</b>) produces results with high specificity and yields. The same experiment was also performed with competitor DNA polymerases: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: PrimeSTAR GXL. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957" href="#collapse1"><span class="caret"></span><strong> Yield and specificity </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-dba95534229571"><div class="container-dba95534229571 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> High yield & specificity </div></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase amplifies a broad range of target lengths with high specificity and yield due to robustness of the enzyme and superior <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Specificity">hot-start technology</a>. The antibody-based Platinum hot-start technology inhibits enzyme activity until the initial PCR denaturation step, preventing nonspecific amplification and primer degradation while allowing greater yield of the target amplicons.</p></div><div class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div class="image parbase section"><div id="cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.low.png/1732033044805.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.medium.png/1732033044805.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.480.medium.png/1732033044805.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.620.high.png/1732033044805.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.full.high.png/1732033044805.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.full.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.480.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.medium.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.620.high.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.320.low.png/1732033044805.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229571/tab/image_21c4/foregroundimg.img.480.medium.png/1732033044805.png" alt='Composite of four gel images. Each gel has five lanes of samples' title='Comparison of performance of DNA polymerases in amplifying DNA targets of various lengths'></picture><style> #cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4 img{ width:100%; max-width: 1459px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4 .image-contain" + "," + "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229571tabimage_21c4 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"></div></div><div style="clear:both"></div><div class="text parbase section"><p><small><b>Figure 6. Versatility across broad range of amplicon lengths. </b>Platinum SuperFi II DNA Polymerase (far left panel) provides high specificity and yield across range of DNA fragments from 0.3 kb to 14 kb amplified from 100 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases: <b>A</b>: Merck KOD™ Hot Start, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: PrimeSTAR GXL. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957" href="#collapse2"><span class="caret"></span><strong> High sensitivity </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-dba95534229572"><div class="container-dba95534229572 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> High sensitivity </div></div><div class="text parbase section"><p>The high sensitivity of Platinum SuperFi II DNA Polymerase enables detection of low-abundance DNA templates. High sensitivity is advantageous in experiments where there is a limited amount of starting material, or the target DNA is in low concentration in the sample.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229572tabtextimage_cee4"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.low.png/1732033044932.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.medium.png/1732033044932.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.480.medium.png/1732033044932.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.620.high.png/1732033044932.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.full.high.png/1732033044932.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.full.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.480.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.medium.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.620.high.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.320.low.png/1732033044932.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229572/tab/textimage_cee4/image.img.480.medium.png/1732033044932.png" alt='Composite of four gel images; each gel has five lanes of samples' title='Comparison of performance of DNA polymerases in amplifying low abundance DNA templates'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229572tabtextimage_cee4 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229572tabtextimage_cee4 img { width: 100%; max-width: 1779px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 7. High sensitivity and reliable amplification from low amounts of input DNA.</b> Platinum SuperFi II DNA Polymerase (far left panel) shows reliable amplification of a 2 kb fragment from 0.4 ng, 2 ng, 10 ng, 50 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane.). The same target was amplified with competitor DNA polymerases: <b>A</b>: PfuUltra™ II Fusion Hot Start, <b>B</b>: HotStar™ HiFidelity DNA Polymerase Kit, <b>C</b>: Expand HiFiPLUS Enzyme Blend. The estimated copy number is ~100 copies per 0.4 ng of human genomic DNA. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957" href="#collapse3"><span class="caret"></span><strong> Inhibitor tolerance </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-dba95534229573"><div class="container-dba95534229573 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> Inhibitor tolerance </div></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase is engineered with a DNA-binding domain resulting in <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Processivity">high processivity</a> and increased tolerance to common PCR inhibitors (<b>Figure 8</b>) such as, hemin, humic acid, and bile salt. </p></div><div class="parsys_column cq-colctrl-lt1"><div class="parsys_column cq-colctrl-lt1-c0"><div class="image parbase section"><div id="cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.low.png/1732033045077.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.medium.png/1732033045077.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.480.medium.png/1732033045077.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.620.high.png/1732033045077.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.full.high.png/1732033045077.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.full.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.480.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.medium.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.620.high.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.320.low.png/1732033045077.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229573/tab/image_e4f9/foregroundimg.img.480.medium.png/1732033045077.png" alt='Composite of five gel images; each gel has four lanes of samples' title='Comparison of performance of DNA polymerases in the presence of common inhibitors'></picture><style> #cq-image-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9 img{ width:100%; max-width: 1614px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9 .image-contain" + "," + "#cq-image-jsp-"+"a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229573tabimage_e4f9 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt1-c1"></div></div><div style="clear:both"></div><div class="text parbase section"><p><small><b>Figure 8. Platinum SuperFi II DNA Polymerase shows high tolerance to common PCR inhibitors. </b>A 2 kb human genomic DNA fragment was amplified from 50 ng of human genomic DNA using Platinum SuperFi II DNA Polymerase or competitor high-fidelity DNA polymerases: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: PrimeSTAR GXL, <b>C</b>: Merck KOD™ Hot Start, and <b>D</b>: KAPA HiFi HotStart PCR Kit in reaction mixtures containing <b>1</b>: no inhibitor, <b>2</b>: humic acid (4 µg/mL), <b>3</b>: hemin (20 µM), and <b>4</b>: bile salt (1 mg/mL). The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957" href="#collapse4"><span class="caret"></span><strong> Benchtop stability </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-dba95534229574"><div class="container-dba95534229574 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><div class="h3"> Benchtop stability </div></div><div class="text parbase section"><p>Extended stability of the Platinum SuperFi II DNA Polymerase enzyme at room temperature (Figure 9) enables high-throughput applications. Its superior Platinum <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Specificity">hot-start technology</a> enables benchtop stability and amplification with high specificity.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229574tabtextimage_7d61"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.full.high.png/1732033045193.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.full.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.320.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.480.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.320.medium.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.620.high.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.320.low.png/1732033045193.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_553422957/container-dba95534229574/tab/textimage_7d61/image.img.480.medium.png/1732033045193.png" alt='Gel image with 8 lanes' title='Comparison of performance of DNA polymerases after reactions were left for 24 hours at room temperature'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229574tabtextimage_7d61 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_553422957container-dba95534229574tabtextimage_7d61 img { width: 100%; max-width: 465px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 9. Assembled reactions with Platinum SuperFi II DNA Polymerase are stable room temperature.</b> A 0.5 kb fragment was amplified form 50 ng of human genomic DNA. PCR reactions were set up and left at room temperature for 0 hr and 24 hr before loading to the <a href="/sg/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/proflex-pcr-system.html">Applied Biosystems ProFlex thermal cycler</a>. Even after 24 hr of room-temperature setup, Platinum SuperFi II DNA Polymerase (<b>lane P</b>) produces results with high specificity and yields. The same experiment was also performed with competitor DNA polymerases: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: PrimeSTAR GXL. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="pcr"> PCR of different templates with Platinum SuperFi II DNA Polymerase </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-dba910501910401">AT- & GC-rich PCR</a></li><li><a data-toggle="tab" href="#container-dba910501910402">Long-range PCR</a></li><li><a data-toggle="tab" href="#container-dba910501910403">Multiplex PCR</a></li><li><a data-toggle="tab" href="#container-dba910501910404">PCR with FFPE DNA</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-dba910501910401" class="tab-pane active"><div class="container-dba910501910401 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> AT & GC-rich PCR </h3></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase amplifies a broad range of sequences with high specificity due to robustness of the enzyme and its specially formulated buffer. The Platinum SuperFi II buffer enables amplification of GC-rich targets without a need for supplementary additives (<b>Figure 10 and 11</b>).</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_5607"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.high.jpg/1732033045440.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.480.high.jpg/1732033045440.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.full.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.480.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.medium.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.620.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.low.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.480.medium.jpg/1732033045440.jpg" alt='Gel image with 15 lanes' title='Amplification of fifteen targets of varying GC content with Platinum SuperFi II DNA polymerase'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_5607 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_5607 img { width: 100%; max-width: 550px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 10. Robust amplification of AT-rich and GC-rich targets by Platinum SuperFi II DNA polymerase.</b> Fifteen targets of varying GC content were amplified from 50 ng of human genomic DNA without any supplementary buffer additives that help with DNA denaturation. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_f7a0"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.low.png/1732033045498.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.medium.png/1732033045498.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.480.medium.png/1732033045498.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.620.high.png/1732033045498.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.full.high.png/1732033045498.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.full.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.480.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.medium.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.620.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.low.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.480.medium.png/1732033045498.png" alt='Composite of four gel images; each gel has four lanes' title='Comparison of various DNA polymerases in amplifying GC-rich targets '></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_f7a0 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_f7a0 img { width: 100%; max-width: 1330px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 11. Enhanced amplification of GC-rich targets. </b>Platinum SuperFi II DNA Polymerase provides high specificity and yield of difficult GC-rich targets (far left panel) without any supplementary DNA-melting additives. Four GC-rich fragments (0.74 kb, 0.58 kb, 0.71 kb, and 0.72 kb in length; GC content indicated above) were amplified from 50 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases according to manufacturer recommended protocols for GC-rich PCR: <b>A</b>: PrimeSTAR GXL DNA Polymerase, <b>B</b>: KAPA HiFi HotStart PCR Kit in specialized reaction buffer for GC rich fragments, <b>C</b>: Merck KOD™ Hot Start DNA Polymerase with 10% DMSO added. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div><div id="container-dba910501910402" class="tab-pane"><div class="container-dba910501910402 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Long-range PCR </h3></div><div class="text parbase section"><p>Due its <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Processivity">high processivity</a> and extremely low error rate, Platinum SuperFi II DNA Polymerase is ideal for accurately amplifying long fragments (up to 20 kb) with high yields and specificity (<b>Figure 12</b>). Amplification of longer targets (up to 40 kb) is possible but may require additional optimization such as using high-quality templates (pure, fresh, and intact) and fresh primer solutions (<b>Figure 13</b>).</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_c0c6"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.high.png/1732033045642.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.480.high.png/1732033045642.png' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.full.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.480.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.medium.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.620.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.low.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.480.medium.png/1732033045642.png" alt='Composite of two gels images, each gel has six lanes' title='Comparison of performance of various DNA polymerases to amplify 20 kb targets'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_c0c6 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_c0c6 img { width: 100%; max-width: 539px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 12. Amplification of long fragments</b>. Platinum SuperFi II DNA Polymerase (<b>lane P</b>) successfully amplifies 20 kb targets from 200 ng of human genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: Merck KOD Hot Start, <b>D</b>: PrimeSTAR GXL, and <b>E</b>: PfuUltra™ II Fusion HotStart. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_91c"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.medium.png/1732033045703.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.480.high.png/1732033045703.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.620.high.png/1732033045703.png' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.full.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.480.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.medium.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.620.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.low.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.480.medium.png/1732033045703.png" alt='Two gel images placed adjacent to each other, each gel has six lanes' title='Comparison of performance of various DNA polymerases to amplify 30 kb and 40 kb targets'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_91c { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_91c img { width: 100%; max-width: 709px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 13. Amplification of targets >20 kb</b>. Platinum SuperFi II DNA Polymerase (<b>lane P</b>) successfully amplifies 30 kb targets and 40 kb targets from 50 ng of <i>E. coli</i> genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: Merck KOD™ Hot Start, <b>D</b>: PrimeSTAR GXL and <b>E</b>: PfuUltra II Fusion HotStart. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div><div id="container-dba910501910403" class="tab-pane"><div class="container-dba910501910403 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Multiplex PCR </h3></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase can multiplex over a broad range of template concentrations in the buffer provided, without the need for significant optimization.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910403tabtextimage_3af6"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.full.high.png/1732033045822.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.full.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.320.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.480.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.320.medium.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.620.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.320.low.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.480.medium.png/1732033045822.png" alt='Gel image with four lanes' title='15 targets amplified by multiplexing with Platinum SuperFi II DNA polymerase'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910403tabtextimage_3af6 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910403tabtextimage_3af6 img { width: 100%; max-width: 252px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><small><b>Figure 14. Multiplex PCR over a broad range of template concentrations using Platinum SuperFi II DNA Polymerase. </b>15 targets (99 bp; 131 bp; 160 bp; 199 bp; 251 bp; 300 bp; 345 bp; 400 bp; 516 bp; 613 bp; 735 bp; 908 bp; 1,005 bp; 1,190 bp; and 1,606 bp) were amplified from 2.5 ng, 25 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane). The molecular weight marker is <a adhocenable="false" href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-dba910501910404" class="tab-pane"><div class="container-dba910501910404 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> FFPE DNA Amplification </h3></div><div class="text parbase section"><p>Due to high sensitivity and inhibitor tolerance, Platinum SuperFi II DNA Polymerase enables efficient amplification of suboptimal quality DNA from formalin fixed paraffin embedded (FFPE) samples.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910404tabtextimage_bb3"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.full.high.png/1732033045918.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.full.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.320.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.480.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.320.medium.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.620.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.320.low.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.480.medium.png/1732033045918.png" alt='Gel image with 6 lanes of samples flanked by lanes of ladder' title='Amplification of DNA from formalin fixed paraffin embedded (FFPE) samples'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910404tabtextimage_bb3 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910404tabtextimage_bb3 img { width: 100%; max-width: 385px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 15. Amplification of FFPE-extracted DNA. </b>Platinum SuperFi II DNA Polymerase successfully amplifies targets up to 0.4 kb from 10 ng of mouse FFPE DNA extracted using <a href="/order/catalog/product/AM1975">Invitrogen RecoverAll Total Nucleic Acid Isolation Kit for FFPE</a>. The molecular weight marker is <a href="/order/catalog/product/10488058">TrackIt 100 bp DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040" href="#collapse1"><span class="caret"></span><strong> AT- & GC-rich PCR </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-dba910501910401"><div class="container-dba910501910401 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> AT & GC-rich PCR </h3></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase amplifies a broad range of sequences with high specificity due to robustness of the enzyme and its specially formulated buffer. The Platinum SuperFi II buffer enables amplification of GC-rich targets without a need for supplementary additives (<b>Figure 10 and 11</b>).</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_5607"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.high.jpg/1732033045440.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.480.high.jpg/1732033045440.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.full.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.480.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.medium.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.620.high.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.320.low.jpg/1732033045440.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_5607/image.img.480.medium.jpg/1732033045440.jpg" alt='Gel image with 15 lanes' title='Amplification of fifteen targets of varying GC content with Platinum SuperFi II DNA polymerase'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_5607 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_5607 img { width: 100%; max-width: 550px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 10. Robust amplification of AT-rich and GC-rich targets by Platinum SuperFi II DNA polymerase.</b> Fifteen targets of varying GC content were amplified from 50 ng of human genomic DNA without any supplementary buffer additives that help with DNA denaturation. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_f7a0"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.low.png/1732033045498.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.medium.png/1732033045498.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.480.medium.png/1732033045498.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.620.high.png/1732033045498.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.full.high.png/1732033045498.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.full.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.480.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.medium.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.620.high.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.320.low.png/1732033045498.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910401/tab/textimage_f7a0/image.img.480.medium.png/1732033045498.png" alt='Composite of four gel images; each gel has four lanes' title='Comparison of various DNA polymerases in amplifying GC-rich targets '></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_f7a0 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910401tabtextimage_f7a0 img { width: 100%; max-width: 1330px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 11. Enhanced amplification of GC-rich targets. </b>Platinum SuperFi II DNA Polymerase provides high specificity and yield of difficult GC-rich targets (far left panel) without any supplementary DNA-melting additives. Four GC-rich fragments (0.74 kb, 0.58 kb, 0.71 kb, and 0.72 kb in length; GC content indicated above) were amplified from 50 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases according to manufacturer recommended protocols for GC-rich PCR: <b>A</b>: PrimeSTAR GXL DNA Polymerase, <b>B</b>: KAPA HiFi HotStart PCR Kit in specialized reaction buffer for GC rich fragments, <b>C</b>: Merck KOD™ Hot Start DNA Polymerase with 10% DMSO added. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040" href="#collapse2"><span class="caret"></span><strong> Long-range PCR </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-dba910501910402"><div class="container-dba910501910402 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Long-range PCR </h3></div><div class="text parbase section"><p>Due its <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/dna-polymerase-characteristics.html#Processivity">high processivity</a> and extremely low error rate, Platinum SuperFi II DNA Polymerase is ideal for accurately amplifying long fragments (up to 20 kb) with high yields and specificity (<b>Figure 12</b>). Amplification of longer targets (up to 40 kb) is possible but may require additional optimization such as using high-quality templates (pure, fresh, and intact) and fresh primer solutions (<b>Figure 13</b>).</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_c0c6"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.high.png/1732033045642.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.480.high.png/1732033045642.png' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.full.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.480.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.medium.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.620.high.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.320.low.png/1732033045642.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_c0c6/image.img.480.medium.png/1732033045642.png" alt='Composite of two gels images, each gel has six lanes' title='Comparison of performance of various DNA polymerases to amplify 20 kb targets'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_c0c6 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_c0c6 img { width: 100%; max-width: 539px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 12. Amplification of long fragments</b>. Platinum SuperFi II DNA Polymerase (<b>lane P</b>) successfully amplifies 20 kb targets from 200 ng of human genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: Merck KOD Hot Start, <b>D</b>: PrimeSTAR GXL, and <b>E</b>: PfuUltra™ II Fusion HotStart. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_91c"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.medium.png/1732033045703.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.480.high.png/1732033045703.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.620.high.png/1732033045703.png' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.full.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.480.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.medium.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.620.high.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.320.low.png/1732033045703.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910402/tab/textimage_91c/image.img.480.medium.png/1732033045703.png" alt='Two gel images placed adjacent to each other, each gel has six lanes' title='Comparison of performance of various DNA polymerases to amplify 30 kb and 40 kb targets'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_91c { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910402tabtextimage_91c img { width: 100%; max-width: 709px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 13. Amplification of targets >20 kb</b>. Platinum SuperFi II DNA Polymerase (<b>lane P</b>) successfully amplifies 30 kb targets and 40 kb targets from 50 ng of <i>E. coli</i> genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: <b>A</b>: Q5 Hot Start High-Fidelity, <b>B</b>: KAPA HiFi HotStart PCR Kit, <b>C</b>: Merck KOD™ Hot Start, <b>D</b>: PrimeSTAR GXL and <b>E</b>: PfuUltra II Fusion HotStart. The molecular weight marker is <a href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div></div><div style="clear:both"></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040" href="#collapse3"><span class="caret"></span><strong> Multiplex PCR </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-dba910501910403"><div class="container-dba910501910403 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Multiplex PCR </h3></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase can multiplex over a broad range of template concentrations in the buffer provided, without the need for significant optimization.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910403tabtextimage_3af6"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.full.high.png/1732033045822.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.full.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.320.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.480.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.320.medium.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.620.high.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.320.low.png/1732033045822.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910403/tab/textimage_3af6/image.img.480.medium.png/1732033045822.png" alt='Gel image with four lanes' title='15 targets amplified by multiplexing with Platinum SuperFi II DNA polymerase'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910403tabtextimage_3af6 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910403tabtextimage_3af6 img { width: 100%; max-width: 252px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><small><b>Figure 14. Multiplex PCR over a broad range of template concentrations using Platinum SuperFi II DNA Polymerase. </b>15 targets (99 bp; 131 bp; 160 bp; 199 bp; 251 bp; 300 bp; 345 bp; 400 bp; 516 bp; 613 bp; 735 bp; 908 bp; 1,005 bp; 1,190 bp; and 1,606 bp) were amplified from 2.5 ng, 25 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane). The molecular weight marker is <a adhocenable="false" href="/order/catalog/product/10488085">TrackIt 1 Kb Plus DNA Ladder</a>.</small></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040" href="#collapse4"><span class="caret"></span><strong> PCR with FFPE DNA </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-dba910501910404"><div class="container-dba910501910404 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> FFPE DNA Amplification </h3></div><div class="text parbase section"><p>Due to high sensitivity and inhibitor tolerance, Platinum SuperFi II DNA Polymerase enables efficient amplification of suboptimal quality DNA from formalin fixed paraffin embedded (FFPE) samples.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910404tabtextimage_bb3"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.full.high.png/1732033045918.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.full.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.320.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.480.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.320.medium.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.620.high.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.320.low.png/1732033045918.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme/jcr:content/MainParsys/tabworkflowcontainer_1050191040/container-dba910501910404/tab/textimage_bb3/image.img.480.medium.png/1732033045918.png" alt='Gel image with 6 lanes of samples flanked by lanes of ladder' title='Amplification of DNA from formalin fixed paraffin embedded (FFPE) samples'></picture></div><style> #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910404tabtextimage_bb3 { max-width: 100%; } #cq-textimage-jsp-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_1050191040container-dba910501910404tabtextimage_bb3 img { width: 100%; max-width: 385px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 15. Amplification of FFPE-extracted DNA. </b>Platinum SuperFi II DNA Polymerase successfully amplifies targets up to 0.4 kb from 10 ng of mouse FFPE DNA extracted using <a href="/order/catalog/product/AM1975">Invitrogen RecoverAll Total Nucleic Acid Isolation Kit for FFPE</a>. The molecular weight marker is <a href="/order/catalog/product/10488058">TrackIt 100 bp DNA Ladder</a>.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="differences"> Differences between Platinum SuperFi II DNA Polymerase and Platinum SuperFi DNA Polymerase </h2></div><div class="text parbase section"><p>Platinum SuperFi II DNA Polymerase comes with a reaction buffer that is specially formulated with iso-stabilizing components. This unique buffer composition offers several advantages: No T<sub>m</sub> calculation for primers, more robust amplification of GC-rich target, enhanced amplification of long sequences, and a <a href="?open=s1t3">universal PCR protocol</a> for high-throughput PCR (<b>Table 1</b>).</p></div><div class="heading section"><h3 id="selection-guide "> Table 1. Comparison of Platinum SuperFi II and Platinum SuperFi DNA Polymerases. </h3></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1989767446"><tbody><tr><th width="30%"> </th><th><p style="text-align: center;"><a href="/order/catalog/product/12361010"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="70%" title="Platinum SuperFi II DNA Polymerase" alt="Product photo of package and three tubes of reagents"><br></a></p><a href="/order/catalog/product/12361010"></a><p style="text-align: center;"><a href="/order/catalog/product/12361010">Platinum SuperFi II DNA Polymerase</a></p></th><th><p style="text-align: center;"><a href="/order/catalog/product/12351010"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi.jpg" width="70%" title="Platinum SuperFi DNA Polymerase" alt="Product photo of package and four tubes of reagents"></a></p><a href="/order/catalog/product/12351010"></a><p style="text-align: center;"><a href="/order/catalog/product/12351010">Platinum SuperFi DNA Polymerase</a></p></th></tr><tr><td><b>Fidelity (vs. <i>Taq</i>)</b></td><td style="text-align: center;">>300x</td><td style="text-align: center;">>300x</td></tr><tr><td><b>Hot-start modification</b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/tm-calculator.html">T<sub>m</sub> calculator</a> needed</b></td><td style="background-color: rgb(243, 243, 243); text-align: center;">No (primers anneal at 60°C)</td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal PCR protocol</a></b></td><td style="background-color: rgb(243, 243, 243); text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>GC-rich amplification</b></td><td style="background-color: rgb(243, 243, 243); text-align: center;">Yes (GC enhancer not required)</td><td style="text-align: center;">Yes (GC enhancer recommended)</td></tr><tr><td><b>Long-range amplification</b></td><td style="background-color: rgb(243, 243, 243); text-align: center;">Up to 20 kb (enhanced performance)</td><td style="text-align: center;">Up to 20 kb</td></tr><tr><td><b>Benchtop stability</b></td><td style="text-align: center;">Up to 24 hr</td><td style="text-align: center;">Up to 24 hr</td></tr><tr><td><b>Inhibitor tolerance</b></td><td style="text-align: center;">High</td><td style="text-align: center;">High</td></tr><tr><td><b>3′ end of amplicons</b></td><td style="text-align: center;">Blunt</td><td style="text-align: center;">Blunt</td></tr><tr><td><b>Certified low level of residual DNA (per 50 μL rxn)</b></td><td style="text-align: center;">≤1 copy of bacterial DNA<br> ≤0.3 copy of human DNA  </td><td style="text-align: center;">≤1 copy of bacterial DNA<br> ≤0.2 copy of human DNA</td></tr><tr><td><b>Formats</b></td><td><p style="text-align: center;">Stand-alone polymerase:<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></p><p style="text-align: center;">Master mix:<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p style="text-align: center;">Green master mix:<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td><td><p style="text-align: center;">Stand-alone polymerase:<br><a href="/order/catalog/product/12351010"><span class="btn btn-primary">Order now</span></a></p><p style="text-align: center;">Master mix:<br><a href="/order/catalog/product/12358010"><span class="btn btn-primary">Order now</span></a><br>  </p></td></tr></tbody></table></div><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/platinum-superfi-ii-and-superfi-benchmarking-data.pdf" class="cta-link" target="_blank"><span class="icon-pdf"></span> Benchmarking data of Platinum SuperFi II and Platinum SuperFi DNA polymerases</a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="references"><br>References </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-dba94185382861">Viral research</a></li><li><a data-toggle="tab" href="#container-dba94185382862">Other microbes</a></li><li><a data-toggle="tab" href="#container-dba94185382863">Metagenomics</a></li><li><a data-toggle="tab" href="#container-dba94185382864">Immunology</a></li><li><a data-toggle="tab" href="#container-dba94185382865">Additional areas</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-dba94185382861" class="tab-pane active"><div class="container-dba94185382861 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1102151543"><tbody><tr><th valign="top" width="30%">Usage</th><th valign="top">Reference</th></tr><tr><td valign="top">Amplification of SARS-CoV-2 whole genome for Illumina sequencing</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/33580111/" target="_blank">Botelho-Souza LF, Nogueira-Lima, FS, Roca TP et al. (2021) SARS-CoV-2 genomic surveillance in Rondônia, Brazilian Western Amazon. <i>Sci Rep</i> 11: 3770. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Target capture and enrichment of SARS-CoV-2 cDNA pools for Illumina sequencing</td><td valign="top" target="_blank"><a href="https://www.medrxiv.org/content/10.1101/2021.01.11.21249265v1.full" target="_blank">Choudhury Y, Cher CY, Wan ZY et al. (2021) A viral fragmentation signature for SARS-CoV-2 in clinical samples correlating with contagiousness. <i>medRxiv</i> doi: https://doi.org/10.1101/2021.01.11.21249265 <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Cloning of SARS-CoV-2 whole genome for functional studies</td><td valign="top" target="_blank"><a href="https://pubmed.ncbi.nlm.nih.gov/33500537/" target="_blank">Kotaki T, Xie X, Shi PY et al. (2021) A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation. <i>Sci Rep</i> 11: 2229. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">RT-PCR of viral genes from human serum samples, followed by Sanger sequencing</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/32873064/" target="_blank">Lan Y, He X, Xin R (2021) Genetic characteristics of HIV-1 CRF12_BF first identified in Guangdong province, China. <i>AIDS Res Hum Retroviruses</i> 37(2):157–161. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Amplification of HIV-1 gene</td><td valign="top"><a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11661/2578317/Label-free-detection-of-mutations-in-the-HIV-genome-using/10.1117/12.2578317.short" target="_blank">Lugongolo MY, Manoto SL, Maphanga C et al. (2021) Label-free detection of mutations in the HIV genome using a surface plasmon resonance biosensor. <i>SPIE BiOS</i> https://doi.org/10.1117/12.2578317 <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top" target="_blank">Amplification of SARS-CoV-2 whole genome for Illumina sequencing</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/32997001/" target="_blank">Nascimento VAD, Corado ALG, Nascimento FOD et al. (2020) Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil. <i>Mem Inst Oswaldo Cruz</i> 115:e200310. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Cloning of SARS-CoV1 and SARS-CoV-2 spike variants for binding assays</td><td valign="top"><a href="https://www.medrxiv.org/content/10.1101/2021.02.05.21251182v1.full-text" target="_blank">Stamatatos L, Czartoski J, Wan YH et al. (2021) Antibodies elicited by SARS-CoV-2 infection and boosted by vaccination neutralize an emerging variant and SARS-CoV-1. <i>medRxiv</i> doi: https://doi.org/10.1101/2021.02.05.21251182 <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Cloning of large viral gene fragments for whole genome assembly</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/33514944/" target="_blank">Xie X, Lokugamage KG, Zhang X et al. (2021) Engineering SARS-CoV-2 using a reverse genetic system. <i>Nat Protoc</i> 16(3):1761–1784. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top" target="_blank">Cloning of SARS-CoV-2 whole genome for functional studies</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/32289263" target="_blank">Xie X, Muruato A, Lokugamage KG, et al. (2020) An Infectious cDNA Clone of SARS-CoV-2. <i>Cell Host Microbe</i> 27(5):841–848. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-dba94185382862" class="tab-pane"><div class="container-dba94185382862 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-521605573"><tbody><tr><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Multiplex PCR for detection of <i>S. aureus</i> genes</td><td><a href="https://www.academia.edu/78921864/Staphylococcus_aureus_nasal_colonization_among_health_care_workers_at_an_Egyptian_tertiary_care_hospital" target="_blank">Allam A, Fakhr A, Mahmoud M et al. (2021) Staphylococcus aureus nasal colonization among health care workers at an Egyptian tertiary care hospital. <i>Microbes and Infectious Diseases</i> 2(1):108–118. <span class="icon icon-outbound"></span></a></td></tr><tr><td>PCR with gDNA and cDNA of fungal samples, followed by Sanger sequencing<p> </p></td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33391201/" target="_blank">Ferrara M, Gallo A, Perrone G et al. (2020) Comparative genomic analysis of ochratoxin A biosynthetic cluster in producing fungi: new evidence of a cyclase gene involvement. <i>Front Microbio</i>l 11:581309. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-dba94185382863" class="tab-pane"><div class="container-dba94185382863 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1170137558"><tbody><tr><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Amplification of bacterial full-length genes, followed by Sanger sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33070091/" target="_blank">Ferrara M, Haidukowski M, D'Imperio M et al. (2021) New insight into microbial degradation of mycotoxins during anaerobic digestion. <i>Waste Manag</i> 119:215–225. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Metabarcoding of fish DNA from pumped deep-sea water</td><td><a href="https://www.sciencedirect.com/science/article/pii/S2215016121000315" target="_blank">Kawato M, Yoshida T, Miya M et al. (2021) Optimization of environmental DNA extraction and amplification methods for metabarcoding of deep-sea fish. <i>MethodsX</i> 8: 101238. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Rare mutation detection by molecular barcoding; fidelity measurement of different DNA polymerases</td><td><a href="https://doi.org/10.1016/j.jmb.2021.167209" target="_blank">Mielinis P, Sukackaite R, Serapinaite A et al. (2021) MuA-based molecular indexing for rare mutation detection by next-generation sequencing. <i>J Mol Bio</i> 443 (19):167209. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-dba94185382864" class="tab-pane"><div class="container-dba94185382864 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1910462871"><tbody><tr><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Cloning of VH and VL regions of an immunoglobulin</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33101296/" target="_blank">Granel J, Lemoine R, Morello E (2020) 4C3 human monoclonal antibody: a proof of concept for non-pathogenic proteinase 3 anti-neutrophil cytoplasmic antibodies in granulomatosis with polyangiitis. <i>Front Immuno</i>l 11:573040. <span class="icon icon-outbound"></span></a></td></tr><tr><td>RT-PCR with human liver and testis samples for microRNA expression analysis, followed by Sanger sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33633774/" target="_blank">Rubino E, Cruciani M, Tchitchek N (2021) Human ubiquitin-specific peptidase 18 is regulated by microRNAs via the 3'untranslated region, a sequence duplicated in long intergenic non-coding RNA genes residing in chr22q11.21. <i>Front Genet</i> 11:627007. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-dba94185382865" class="tab-pane"><div class="container-dba94185382865 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-4381021"><tbody><tr><th width="15%">Research areas</th><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Agrigenomics</td><td>RT-PCR with cDNA from hen tissues for gene expression analysis</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33405082/" target="_blank">Ma Y, Shi Y, Wu Q et al. (2021) Epigallocatechin-3-gallate alleviates vanadium-induced reduction of antioxidant capacity via Keap1-Nrf2-sMaf pathway in the liver, kidney, and ovary of laying hens. <i>Biol Trace Elem Res</i> doi: 10.1007/s12011-020-02398-z. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Cancer research</td><td>Generation of dsDNA barcodes for a lentiviral plasmid library, followed by the Golden Gate assembly</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/32187448/" target="_blank">Akimov Y, Bulanova D, Timonen S (2020) Improved detection of differentially represented DNA barcodes for high-throughput clonal phenomics. <i>Mol Syst Biol</i> 16(3):e9195. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Population genetics</td><td>Distinguishing diplotypes by PCR, followed by Sanger sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33658540/" target="_blank">Mlakar V, Curtis PH, Armengol M et al. (2021) The analysis of GSTA1 promoter genetic and functional diversity of human populations. <i>Sci Rep</i> 11(1):5038. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Proteomics</td><td>Generation of Y2H library templates for high-throughput NGS sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/32442380/" target="_blank">Yu Q, Hu Y, Su J (2020) Evaluation of a yeast two-hybrid library by high-throughput sequencing. <i>J Proteome Res</i> 19(8):3567–3572. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Reproductive health</td><td>RT-PCR with human primary leukocytes for gene expression analysis</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/32353856/" target="_blank">Bilal MY, Dambaeva S, Brownstein D et al. (2020) Iodide transporters in the endometrium: a potential diagnostic marker for women with recurrent pregnancy failures. <i>Med Princ Pract</i> 29(5):412–421. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_418538286"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_418538286" href="#collapse1"><span class="caret"></span><strong> Viral research </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-dba94185382861"><div class="container-dba94185382861 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1985139567"><tbody><tr><th valign="top" width="30%">Usage</th><th valign="top">Reference</th></tr><tr><td valign="top">Amplification of SARS-CoV-2 whole genome for Illumina sequencing</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/33580111/" target="_blank">Botelho-Souza LF, Nogueira-Lima, FS, Roca TP et al. (2021) SARS-CoV-2 genomic surveillance in Rondônia, Brazilian Western Amazon. <i>Sci Rep</i> 11: 3770. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Target capture and enrichment of SARS-CoV-2 cDNA pools for Illumina sequencing</td><td valign="top" target="_blank"><a href="https://www.medrxiv.org/content/10.1101/2021.01.11.21249265v1.full" target="_blank">Choudhury Y, Cher CY, Wan ZY et al. (2021) A viral fragmentation signature for SARS-CoV-2 in clinical samples correlating with contagiousness. <i>medRxiv</i> doi: https://doi.org/10.1101/2021.01.11.21249265 <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Cloning of SARS-CoV-2 whole genome for functional studies</td><td valign="top" target="_blank"><a href="https://pubmed.ncbi.nlm.nih.gov/33500537/" target="_blank">Kotaki T, Xie X, Shi PY et al. (2021) A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation. <i>Sci Rep</i> 11: 2229. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">RT-PCR of viral genes from human serum samples, followed by Sanger sequencing</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/32873064/" target="_blank">Lan Y, He X, Xin R (2021) Genetic characteristics of HIV-1 CRF12_BF first identified in Guangdong province, China. <i>AIDS Res Hum Retroviruses</i> 37(2):157–161. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Amplification of HIV-1 gene</td><td valign="top"><a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11661/2578317/Label-free-detection-of-mutations-in-the-HIV-genome-using/10.1117/12.2578317.short" target="_blank">Lugongolo MY, Manoto SL, Maphanga C et al. (2021) Label-free detection of mutations in the HIV genome using a surface plasmon resonance biosensor. <i>SPIE BiOS</i> https://doi.org/10.1117/12.2578317 <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top" target="_blank">Amplification of SARS-CoV-2 whole genome for Illumina sequencing</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/32997001/" target="_blank">Nascimento VAD, Corado ALG, Nascimento FOD et al. (2020) Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil. <i>Mem Inst Oswaldo Cruz</i> 115:e200310. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Cloning of SARS-CoV1 and SARS-CoV-2 spike variants for binding assays</td><td valign="top"><a href="https://www.medrxiv.org/content/10.1101/2021.02.05.21251182v1.full-text" target="_blank">Stamatatos L, Czartoski J, Wan YH et al. (2021) Antibodies elicited by SARS-CoV-2 infection and boosted by vaccination neutralize an emerging variant and SARS-CoV-1. <i>medRxiv</i> doi: https://doi.org/10.1101/2021.02.05.21251182 <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top">Cloning of large viral gene fragments for whole genome assembly</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/33514944/" target="_blank">Xie X, Lokugamage KG, Zhang X et al. (2021) Engineering SARS-CoV-2 using a reverse genetic system. <i>Nat Protoc</i> 16(3):1761–1784. <span class="icon icon-outbound"></span></a></td></tr><tr><td valign="top" target="_blank">Cloning of SARS-CoV-2 whole genome for functional studies</td><td valign="top"><a href="https://pubmed.ncbi.nlm.nih.gov/32289263" target="_blank">Xie X, Muruato A, Lokugamage KG, et al. (2020) An Infectious cDNA Clone of SARS-CoV-2. <i>Cell Host Microbe</i> 27(5):841–848. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_418538286" href="#collapse2"><span class="caret"></span><strong> Other microbes </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-dba94185382862"><div class="container-dba94185382862 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1213203836"><tbody><tr><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Multiplex PCR for detection of <i>S. aureus</i> genes</td><td><a href="https://www.academia.edu/78921864/Staphylococcus_aureus_nasal_colonization_among_health_care_workers_at_an_Egyptian_tertiary_care_hospital" target="_blank">Allam A, Fakhr A, Mahmoud M et al. (2021) Staphylococcus aureus nasal colonization among health care workers at an Egyptian tertiary care hospital. <i>Microbes and Infectious Diseases</i> 2(1):108–118. <span class="icon icon-outbound"></span></a></td></tr><tr><td>PCR with gDNA and cDNA of fungal samples, followed by Sanger sequencing<p> </p></td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33391201/" target="_blank">Ferrara M, Gallo A, Perrone G et al. (2020) Comparative genomic analysis of ochratoxin A biosynthetic cluster in producing fungi: new evidence of a cyclase gene involvement. <i>Front Microbio</i>l 11:581309. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_418538286" href="#collapse3"><span class="caret"></span><strong> Metagenomics </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-dba94185382863"><div class="container-dba94185382863 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-342991792"><tbody><tr><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Amplification of bacterial full-length genes, followed by Sanger sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33070091/" target="_blank">Ferrara M, Haidukowski M, D'Imperio M et al. (2021) New insight into microbial degradation of mycotoxins during anaerobic digestion. <i>Waste Manag</i> 119:215–225. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Metabarcoding of fish DNA from pumped deep-sea water</td><td><a href="https://www.sciencedirect.com/science/article/pii/S2215016121000315" target="_blank">Kawato M, Yoshida T, Miya M et al. (2021) Optimization of environmental DNA extraction and amplification methods for metabarcoding of deep-sea fish. <i>MethodsX</i> 8: 101238. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Rare mutation detection by molecular barcoding; fidelity measurement of different DNA polymerases</td><td><a href="https://doi.org/10.1016/j.jmb.2021.167209" target="_blank">Mielinis P, Sukackaite R, Serapinaite A et al. (2021) MuA-based molecular indexing for rare mutation detection by next-generation sequencing. <i>J Mol Bio</i> 443 (19):167209. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_418538286" href="#collapse4"><span class="caret"></span><strong> Immunology </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-dba94185382864"><div class="container-dba94185382864 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--2145608739"><tbody><tr><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Cloning of VH and VL regions of an immunoglobulin</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33101296/" target="_blank">Granel J, Lemoine R, Morello E (2020) 4C3 human monoclonal antibody: a proof of concept for non-pathogenic proteinase 3 anti-neutrophil cytoplasmic antibodies in granulomatosis with polyangiitis. <i>Front Immuno</i>l 11:573040. <span class="icon icon-outbound"></span></a></td></tr><tr><td>RT-PCR with human liver and testis samples for microRNA expression analysis, followed by Sanger sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33633774/" target="_blank">Rubino E, Cruciani M, Tchitchek N (2021) Human ubiquitin-specific peptidase 18 is regulated by microRNAs via the 3'untranslated region, a sequence duplicated in long intergenic non-coding RNA genes residing in chr22q11.21. <i>Front Genet</i> 11:627007. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-a3fdddd6-a256-4681-9367-3153f95318dbMainParsystabworkflowcontainer_418538286" href="#collapse5"><span class="caret"></span><strong> Additional areas </strong></a></div><div id="collapse5" class="accordion-body collapse "><div class="accordion-inner container-dba94185382865"><div class="container-dba94185382865 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-373833427"><tbody><tr><th width="15%">Research areas</th><th width="30%">Usage</th><th>Reference</th></tr><tr><td>Agrigenomics</td><td>RT-PCR with cDNA from hen tissues for gene expression analysis</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33405082/" target="_blank">Ma Y, Shi Y, Wu Q et al. (2021) Epigallocatechin-3-gallate alleviates vanadium-induced reduction of antioxidant capacity via Keap1-Nrf2-sMaf pathway in the liver, kidney, and ovary of laying hens. <i>Biol Trace Elem Res</i> doi: 10.1007/s12011-020-02398-z. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Cancer research</td><td>Generation of dsDNA barcodes for a lentiviral plasmid library, followed by the Golden Gate assembly</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/32187448/" target="_blank">Akimov Y, Bulanova D, Timonen S (2020) Improved detection of differentially represented DNA barcodes for high-throughput clonal phenomics. <i>Mol Syst Biol</i> 16(3):e9195. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Population genetics</td><td>Distinguishing diplotypes by PCR, followed by Sanger sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/33658540/" target="_blank">Mlakar V, Curtis PH, Armengol M et al. (2021) The analysis of GSTA1 promoter genetic and functional diversity of human populations. <i>Sci Rep</i> 11(1):5038. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Proteomics</td><td>Generation of Y2H library templates for high-throughput NGS sequencing</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/32442380/" target="_blank">Yu Q, Hu Y, Su J (2020) Evaluation of a yeast two-hybrid library by high-throughput sequencing. <i>J Proteome Res</i> 19(8):3567–3572. <span class="icon icon-outbound"></span></a></td></tr><tr><td>Reproductive health</td><td>RT-PCR with human primary leukocytes for gene expression analysis</td><td><a href="https://pubmed.ncbi.nlm.nih.gov/32353856/" target="_blank">Bilal MY, Dambaeva S, Brownstein D et al. (2020) Iodide transporters in the endometrium: a potential diagnostic marker for women with recurrent pregnancy failures. <i>Med Princ Pract</i> 29(5):412–421. <span class="icon icon-outbound"></span></a></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div></div></div><div class="LTRawHTML section"><style> .modal-backdrop { background-color: transparent; opacity:0.1; } .brightcoveplayer { cursor:pointer; } .benefit-icon { padding: 8px 20px 8px 45px; margin-bottom: 22px; text-shadow: 0 1px 0 rgba(255, 255, 255, 0.5); background-position: 5px 10px; background-repeat: no-repeat; background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/specificity.png); color: #333333; background-size: 35px 35px;} .bIco1 {background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/specificity.png)} .bIco2 {background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/pcr-runs.png)} .bIco3 {background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/multiplex.png)} .bIco4 {background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/pipette.png)} .bIco5 {background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/optimization.png)} .bIco6 {background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/robust.png)} .bIco7 {background-image: url(/content/dam/LifeTech/global/life-sciences/molecular-biology/images/1020/automation.png)} </style></div><div class="LTRawHTML section"><style> .modal-backdrop { background-color: transparent; opacity:0.1; } </style></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h2> Resources for Platinum SuperFi II DNA polymerase </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><h3 id="resources"> Flyers </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><ul class="the-icons clearfix"><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/platinum-superfi-dna-polymerase-flyer.pdf" target="_blank">Platinum SuperFi II DNA Polymerase</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/PCR-enzymes-sample-prep-kits-microbiome.pdf" target="_blank">Improve microbiome studies</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="heading section"><h3 id="resources"> Manuals/Protocols </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A4"><div class="text parbase section"><ul class="the-icons clearfix"><li><span class=" icon-lt-pdf"></span>Protocol: <a href="https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/mutagenesis-geneart-tech-note.pdf" target="_blank">Site-directed mutagenesis using the GeneArt Gibson Assembly HiFi Cloning Kit</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018859_Platinum_SuperFi_II_DNA_Pol_UG.pdf" target="_blank">Platinum SuperFi II DNA Polymerase</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018860_Platinum_SuperFi_II_PCR_MM_UG.pdf" target="_blank">Platinum SuperFi II PCR Master Mix</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018861_Platinum_SuperFi_II_Green_PCR_MM_UG.pdf" target="_blank">Platinum SuperFi II Green PCR Master Mix</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="A5"><div class="heading section"><h3 id="resources"> Application notes </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A6"><div class="text parbase section"><ul class="the-icons clearfix"><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/site-directed-mutagenesis-platinum-superfi-app-note.pdf" target="_blank">Site-directed mutagenesis</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/direct-pcr-blood-superfi-app-note.pdf" target="_blank">Direct PCR with blood</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/multiplex-pcr-platinum-superfi-app-note.pdf" target="_blank">Multiplex PCR</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/pcr-bacteria-yeast-platinum-superfi-dna-polymerase-app-note.pdf" target="_blank">Colony PCR</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/amplification-dna-sequences-platinum-superfi-ii-dna-polymerase-app-note.pdf" target="_blank">Successful amplification of AT-rich DNA sequences with Platinum SuperFi II DNA Polymerase</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><h3 id="resources"> White papers </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><ul class="the-icons clearfix"><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/gibson-assembly-ex-cloning-large-complex-assemblies-white-paper.pdf" target="_blank">Assembly of large PCR fragments by the Gibson Assembly method</a></li><li><span class=" icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/gibson-assembly-hifi-homology-oligonucleotide-stitching-white-paper.pdf" target="_blank">Assembly of one or more PCR fragments using stitching oligonucleotides</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="heading section"><h3 id="resources"> Spotlight article </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B4"><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Simplifying optimization of PCR annealing</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well well-white well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="text parbase section"><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" id="table--438043477"><tbody><tr><td width="30%" style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/icon-education.svg" width="80%" title="PCR enzymes education" alt="Education icon"></td><td><h3> <a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes.html">PCR enzymes education resources</a></h3></td></tr><tr><td colspan="2">Access PCR learning resources—a knowledge base of all things PCR.</td></tr></tbody></table></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="text parbase section"><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" width="100%" id="table--1196448884"><tbody><tr><td width="24%" style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/icon-webinars.svg" title="Webinars" alt="Webinar icon"></td><td><h3><a href="/sg/en/home/brands/invitrogen/molecular-biology-technologies/pcr-webinars.html">Molecular biology webinars</a></h3></td></tr><tr><td colspan="2">View our webinars for current date to learn more about molecular biology topics.</td></tr></tbody></table></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="text parbase section"><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" width="100%" id="table--1346885731"><tbody><tr><td width="24%" style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/icon-videos.svg" title="Videos" alt="Video icon"></td><td><h3><a href="/sg/en/home/technical-resources/technical-reference-library/pcr-cdna-synthesis-support-center.html" style="text-align: center;">PCR enzymes videos</a></h3></td></tr><tr><td colspan="2">Discover product features and tips to optimize your PCR experiments.</td></tr></tbody></table></div></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="text parbase section"><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" width="100%" id="table--285232554"><tbody><tr><td width="30%" style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/icon-contact-us.svg" width="70%" title="Support center" alt="Tools icon"></td><td><h3><a href="/sg/en/home/technical-resources/technical-reference-library/pcr-cdna-synthesis-support-center.html">Support center</a></h3></td></tr><tr><td colspan="2">Find tips, troubleshooting help, and resources for your end-point PCR and cDNA applications.</td></tr></tbody></table></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="text parbase section"><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" width="100%" id="table-1879400515"><tbody><tr><td width="30%" style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/icon-support-center.svg" width="70%" title="Contact us" alt="Computer mouse icon"></td><td><h3><a href="/sg/en/home/technical-resources/contact-us.html?enableChat=true">Contact us</a></h3></td></tr><tr><td colspan="2">Email or call our Technical Application Scientists for questions regarding PCR enzymes and master mixes.</td></tr></tbody></table></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="text parbase section"><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" width="100%" id="table-1277737971"><tbody><tr><td width="30%" style="text-align: center;"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/icon-oem-and-custom.svg" width="70%" title="OEM and custom PCR products" alt="Check icon"></td><td><h3><a href="/sg/en/home/about-us/partnering-licensing/oem-commercial-supply/life-science-tools.html">OEM and custom PCR products</a></h3></td></tr><tr><td colspan="2">Discover custom manufacturing solutions, product labeling, and packaging capabilities tailored to your specifications.</td></tr></tbody></table></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="reference parbase 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