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Ziino</title> <meta name="description" content="Search results for: Graziella Ziino"> <meta name="keywords" content="Graziella Ziino"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Graziella Ziino"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 7</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Graziella Ziino</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Rapid Identification of Thermophilic Campylobacter Species from Retail Poultry Meat Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Graziella%20Ziino">Graziella Ziino</a>, <a href="https://publications.waset.org/abstracts/search?q=Filippo%20Giarratana"> Filippo Giarratana</a>, <a href="https://publications.waset.org/abstracts/search?q=Stefania%20Maria%20Marotta"> Stefania Maria Marotta</a>, <a href="https://publications.waset.org/abstracts/search?q=Alessandro%20Giuffrida"> Alessandro Giuffrida</a>, <a href="https://publications.waset.org/abstracts/search?q=Antonio%20Panebianco"> Antonio Panebianco</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In Europe, North America and Japan, campylobacteriosis is one of the leading food-borne bacterial illnesses, often related to the consumption of poultry meats and/or by-products. The aim of this study was the evaluation of Campylobacter contamination of poultry meats marketed in Sicily (Italy) using both traditional methods and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). MALDI-TOF MS is considered a promising rapid (less than 1 hour) identification method for food borne pathogens bacteria. One hundred chicken and turkey meat preparations (no. 68 hamburgers, no. 21 raw sausages, no. 4 meatballs and no. 7 meat rolls) were taken from different butcher’s shops and large scale retailers and submitted to detection/enumeration of Campylobacter spp. according to EN ISO 10272-1:2006 and EN ISO 10272-2:2006. Campylobacter spp. was detected with general low counts in 44 samples (44%), of which 30 from large scale retailers and 14 from butcher’s shops. Chicken meats were significantly more contaminated than turkey meats. Among the preparations, Campylobacter spp. was found in 85.71% of meat rolls, 50% of meatballs, 44.12% of hamburgers and 28.57% of raw sausages. A total of 100 strains, 2-3 from each positive samples, were isolated for the identification by phenotypic, biomolecular and MALDI-TOF MS methods. C. jejuni was the predominant strains (63%), followed by C. coli (33%) and C. lari (4%). MALDI-TOF MS correctly identified 98% of the strains at the species level, only 1% of the tested strains were not identified. In the last 1%, a mixture of two different species was mixed in the same sample and MALDI-TOF MS correctly identified at least one of the strains. Considering the importance of rapid identification of pathogens in the food matrix, this method is highly recommended for the identification of suspected colonies of Campylobacteria. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=campylobacter%20spp." title="campylobacter spp.">campylobacter spp.</a>, <a href="https://publications.waset.org/abstracts/search?q=Food%20Microbiology" title=" Food Microbiology"> Food Microbiology</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix-assisted%20laser%20desorption%20ionization-time%20of%20flight%20mass%20spectrometry" title=" matrix-assisted laser desorption ionization-time of flight mass spectrometry"> matrix-assisted laser desorption ionization-time of flight mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=rapid%20microbial%20identification" title=" rapid microbial identification"> rapid microbial identification</a> </p> <a href="https://publications.waset.org/abstracts/71541/rapid-identification-of-thermophilic-campylobacter-species-from-retail-poultry-meat-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/71541.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">292</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Detection and Quantification of Viable but Not Culturable Vibrio Parahaemolyticus in Frozen Bivalve Molluscs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eleonora%20Di%20Salvo">Eleonora Di Salvo</a>, <a href="https://publications.waset.org/abstracts/search?q=Antonio%20Panebianco"> Antonio Panebianco</a>, <a href="https://publications.waset.org/abstracts/search?q=Graziella%20Ziino"> Graziella Ziino</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Vibrio parahaemolyticus is a human pathogen that is widely distributed in marine environments. It is frequently isolated from raw seafood, particularly shellfish. Consumption of raw or undercooked seafood contaminated with V. parahaemolyticus may lead to acute gastroenteritis. Vibrio spp. has excellent resistance to low temperatures so it can be found in frozen products for a long time. Recently, the viable but non-culturable state (VBNC) of bacteria has attracted great attention, and more than 85 species of bacteria have been demonstrated to be capable of entering this state. VBNC cells cannot grow in conventional culture medium but are viable and maintain metabolic activity, which may constitute an unrecognized source of food contamination and infection. Also V. parahaemolyticus could exist in VBNC state under nutrient starvation or low-temperature conditions. Aim: The aim of the present study was to optimize methods and investigate V. parahaemolyticus VBNC cells and their presence in frozen bivalve molluscs, regularly marketed. Materials and Methods: propidium monoazide (PMA) was integrated with real-time polymerase chain reaction (qPCR) targeting the tl gene to detect and quantify V. parahaemolyticus in the VBNC state. PMA-qPCR resulted highly specific to V. parahaemolyticus with a limit of detection (LOD) of 10-1 log CFU/mL in pure bacterial culture. A standard curve for V. parahaemolyticus cell concentrations was established with the correlation coefficient of 0.9999 at the linear range of 1.0 to 8.0 log CFU/mL. A total of 77 samples of frozen bivalve molluscs (35 mussels; 42 clams) were subsequently subjected to the qualitative (on alkaline phosphate buffer solution) and quantitative research of V. parahaemolyticus on thiosulfate-citrate-bile salts-sucrose (TCBS) agar (DIFCO) NaCl 2.5%, and incubation at 30°C for 24-48 hours. Real-time PCR was conducted on homogenate samples, in duplicate, with and without propidium monoazide (PMA) dye, and exposed for 45 min under halogen lights (650 W). Total DNA was extracted from cell suspension in homogenate samples according to bolliture protocol. The Real-time PCR was conducted with species-specific primers for V. parahaemolitycus. The RT-PCR was performed in a final volume of 20 µL, containing 10 µL of SYBR Green Mixture (Applied Biosystems), 2 µL of template DNA, 2 µL of each primer (final concentration 0.6 mM), and H2O 4 µL. The qPCR was carried out on CFX96 TouchTM (Bio-Rad, USA). Results: All samples were negative both to the quantitative and qualitative detection of V. parahaemolyticus by the classical culturing technique. The PMA-qPCR let us individuating VBNC V. parahaemolyticus in the 20,78% of the samples evaluated with a value between the Log 10-1 and Log 10-3 CFU/g. Only clams samples were positive for PMA-qPCR detection. Conclusion: The present research is the first evaluating PMA-qPCR assay for detection of VBNC V. parahaemolyticus in bivalve molluscs samples, and the used method was applicable to the rapid control of marketed bivalve molluscs. We strongly recommend to use of PMA-qPCR in order to identify VBNC forms, undetectable by the classic microbiological methods. A precise knowledge of the V.parahaemolyticus in a VBNC form is fundamental for the correct risk assessment not only in bivalve molluscs but also in other seafood. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=food%20safety" title="food safety">food safety</a>, <a href="https://publications.waset.org/abstracts/search?q=frozen%20bivalve%20molluscs" title=" frozen bivalve molluscs"> frozen bivalve molluscs</a>, <a href="https://publications.waset.org/abstracts/search?q=PMA%20dye" title=" PMA dye"> PMA dye</a>, <a href="https://publications.waset.org/abstracts/search?q=Real-time%20PCR" title=" Real-time PCR"> Real-time PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=VBNC%20state" title=" VBNC state"> VBNC state</a>, <a href="https://publications.waset.org/abstracts/search?q=Vibrio%20parahaemolyticus" title=" Vibrio parahaemolyticus"> Vibrio parahaemolyticus</a> </p> <a href="https://publications.waset.org/abstracts/135106/detection-and-quantification-of-viable-but-not-culturable-vibrio-parahaemolyticus-in-frozen-bivalve-molluscs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/135106.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> A Case Study at Lara's Landfill: Solid Waste Management and Energy Recovery</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kelly%20Danielly%20Da%20Silva%20Alcantara">Kelly Danielly Da Silva Alcantara</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Fernando%20Molina%20Junqueira"> Daniel Fernando Molina Junqueira</a>, <a href="https://publications.waset.org/abstracts/search?q=Graziella%20Colato%20Antonio"> Graziella Colato Antonio</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Law No. 12,305/10, established by the National Solid Waste Policy (PNRS), provides major changes in the management and managing scenario of solid waste in Brazil. The PNRS established since changes from population behavior as environmental and the consciousness and commitment of the companies with the waste produced. The objective of this project is to conduct a benchmarking study of the management models of Waste Management Municipal Solid (MSW) in national and international levels emphasizing especially in the European Union (Portugal, France and Germany), which are reference countries in energy development, sustainability and consequently recovery of waste generated. The management that encompasses all stages that are included in this sector will be analyzed by benchmarking, as the collection, transportation, processing/treatment and final disposal of waste. Considering the needs to produce clean energy in Brazil, this study will allow the determination to the best treatment of the waste in order to reduce the amount of waste and increase the lifetime of the landfill. Finally, it intends to identify the energy recovery potential through a study analysis of economic viability, energy and sustainable based on a holistic approach. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=benchmarking" title="benchmarking">benchmarking</a>, <a href="https://publications.waset.org/abstracts/search?q=energy%20recovery" title=" energy recovery"> energy recovery</a>, <a href="https://publications.waset.org/abstracts/search?q=landfill" title=" landfill"> landfill</a>, <a href="https://publications.waset.org/abstracts/search?q=municipal%20solid%20waste" title=" municipal solid waste"> municipal solid waste</a> </p> <a href="https://publications.waset.org/abstracts/30758/a-case-study-at-laras-landfill-solid-waste-management-and-energy-recovery" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30758.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">426</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Skin Substitutes for Wound Healing: An Advanced Formulation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pennisi%20Stefania">Pennisi Stefania</a>, <a href="https://publications.waset.org/abstracts/search?q=Giuffrida%20Graziella"> Giuffrida Graziella</a>, <a href="https://publications.waset.org/abstracts/search?q=Coppa%20Federica"> Coppa Federica</a>, <a href="https://publications.waset.org/abstracts/search?q=Iannello%20Giulia"> Iannello Giulia</a>, <a href="https://publications.waset.org/abstracts/search?q=Cartelli%20Simone"> Cartelli Simone</a>, <a href="https://publications.waset.org/abstracts/search?q=Lo%20Faro%20Riccardo"> Lo Faro Riccardo</a>, <a href="https://publications.waset.org/abstracts/search?q=Ferruggia%20Greta"> Ferruggia Greta</a>, <a href="https://publications.waset.org/abstracts/search?q=Brundo%20Maria%20Violetta"> Brundo Maria Violetta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Tissue engineering aims to develop advanced medical devices to restore normal functions of damaged tissue. These devices, even more effective than conventional methods, are called skin substitutes and are configured as drugs to be applied to the damaged area, to heal extensive and deep wounds which could otherwise lead to chronic wounds lasting over time. Among the variety of commercially available skin substitutes, those that have proven to be most effective are those consisting of a bilayer scaffold. The aim of our research was to design a skin substitute which can promote cell proliferation, cell migration and angiogenesis, and which can guarantee timely closure of the wound with satisfactory aesthetic results, in order to avoid the patient excessive pain, risk of contracting infections and long-term hospitalization. The product was tested in vitro using the Scratch Assay. The assay was carried out both on the matrix modified with hyaluronic acid and on the matrix based only on collagen. In both cases, after 48 hours of exposure the wound scratch was almost completely closed in treated cells compared to untreated control. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=collagen" title="collagen">collagen</a>, <a href="https://publications.waset.org/abstracts/search?q=hyaluronic%20acid" title=" hyaluronic acid"> hyaluronic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=scratch-%20wound-healing%20assay" title=" scratch- wound-healing assay"> scratch- wound-healing assay</a>, <a href="https://publications.waset.org/abstracts/search?q=tissue%20regeneration" title=" tissue regeneration"> tissue regeneration</a> </p> <a href="https://publications.waset.org/abstracts/187381/skin-substitutes-for-wound-healing-an-advanced-formulation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/187381.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">25</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Effectiveness of Opuntia ficus indica Cladodes Extract for Wound-Healing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Giuffrida%20Graziella">Giuffrida Graziella</a>, <a href="https://publications.waset.org/abstracts/search?q=Pennisi%20Stefania"> Pennisi Stefania</a>, <a href="https://publications.waset.org/abstracts/search?q=Coppa%20Federica"> Coppa Federica</a>, <a href="https://publications.waset.org/abstracts/search?q=Iannello%20Giulia"> Iannello Giulia</a>, <a href="https://publications.waset.org/abstracts/search?q=Cartelli%20Simone"> Cartelli Simone</a>, <a href="https://publications.waset.org/abstracts/search?q=Lo%20Faro%20Riccardo"> Lo Faro Riccardo</a>, <a href="https://publications.waset.org/abstracts/search?q=Ferruggia%20Greta"> Ferruggia Greta</a>, <a href="https://publications.waset.org/abstracts/search?q=Brundo%20Maria%20Violetta"> Brundo Maria Violetta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cladode chemical composition may vary according to soil factors, cultivation season, and plant age. The primary metabolites of cladodes are water, carbohydrates, and proteins. The carbohydrates in cladodes are divided into two types: structural and storage. Polysaccharides from Opuntia ficus‐indica (L.) Mill plants build molecular networks with the capacity to retain water; thus, they act as mucoprotective agents. Mucilage is the main polysaccharide of cladodes; it contains polymers of β‐d‐galacturonic acid bound in positions (1–4) and traces of R‐linked l‐rhamnose (1-2). Mucilage regulates both the cell water content during prolonged drought and the calcium flux in the plant cells. The in vitro analysis of keratinocytes in monolayer, through the scratch-wound-healing assay, provided promising results. After 48 hours of exposure, the wound scratch was almost completely closed in cells treated with cladode extract. After 72 hours, the treated cells reached complete confluence, while in the untreated cells (negative control) the confluence was reached after 96 hours. We also added a positive control group of cells treated with colchicine, which inhibited wound closure for a more comprehensive analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cladodes" title="cladodes">cladodes</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolites" title=" metabolites"> metabolites</a>, <a href="https://publications.waset.org/abstracts/search?q=polysaccharide" title=" polysaccharide"> polysaccharide</a>, <a href="https://publications.waset.org/abstracts/search?q=scratch-wound-healing%20assay" title=" scratch-wound-healing assay"> scratch-wound-healing assay</a> </p> <a href="https://publications.waset.org/abstracts/187380/effectiveness-of-opuntia-ficus-indica-cladodes-extract-for-wound-healing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/187380.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">53</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Food Supplements and Natural Products to Slow Down Biological Aging</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Coppa%20Federica">Coppa Federica</a>, <a href="https://publications.waset.org/abstracts/search?q=Iannello%20Giulia"> Iannello Giulia</a>, <a href="https://publications.waset.org/abstracts/search?q=Pennisi%20Stefania"> Pennisi Stefania</a>, <a href="https://publications.waset.org/abstracts/search?q=Giuffrida%20Graziella"> Giuffrida Graziella</a>, <a href="https://publications.waset.org/abstracts/search?q=Lo%20Faro%20Riccardo"> Lo Faro Riccardo</a>, <a href="https://publications.waset.org/abstracts/search?q=Cartelli%20Simone"> Cartelli Simone</a>, <a href="https://publications.waset.org/abstracts/search?q=Ferruggia%20Greta"> Ferruggia Greta</a>, <a href="https://publications.waset.org/abstracts/search?q=Brundo%20Maria%20Violetta"> Brundo Maria Violetta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In recent years, a new field of basic research has emerged: the biology and physiology of extracellular vesicles and their application in diagnostics and therapy. In particular, exosomes attract the scientific community as nanovesicles of endosomal origin, which can be secreted by a variety of cells and are found in all biological fluids. Exosomes have recently gained attention also in the cosmetic field: in fact, they are used in creams, serums and masks for topical use, proving to have a series of therapeutic and anti-aging benefits. To date, the oral administration of exosomes is the subject of attention because it represents a non-invasive and efficient method for delivering bioactive molecules into the intestine. We decided to focus our research on the creation of a food supplement that contains various bioactive factors, vitamins, and a new technology called AMPLEX PLUS, containing a mixture of 20 different biologically active factors (GF20) and exosomes isolated and purified from bovine colostrum. We have demonstrated in vitro that this new supplement acts on telomerase, slowing down cell aging. Amplex plus increased the proliferation rate of cells and the addition of it reduced the rate of telomere shortening. Under oxidative stress conditions (H2O2 – induced), the TSR increased; however, treatment with colostrum appeared to attenuate this increase. In particular, after 2 weeks of treatment, AMPLEX plus increased the proliferation rate of cells and exerted a protective effect on telomere length erosion, reducing the rate of its shortening. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=AMPLEX%20PLUS" title="AMPLEX PLUS">AMPLEX PLUS</a>, <a href="https://publications.waset.org/abstracts/search?q=colostrum" title=" colostrum"> colostrum</a>, <a href="https://publications.waset.org/abstracts/search?q=exosomes" title=" exosomes"> exosomes</a>, <a href="https://publications.waset.org/abstracts/search?q=telomerase" title=" telomerase"> telomerase</a> </p> <a href="https://publications.waset.org/abstracts/187383/food-supplements-and-natural-products-to-slow-down-biological-aging" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/187383.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">52</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Comparative Analysis of Short and Long Term Salt Stress on the Photosynthetic Apparatus and Chloroplast Ultrastructure of Thellungiella salsuginea </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rahma%20Goussi">Rahma Goussi</a>, <a href="https://publications.waset.org/abstracts/search?q=Walid%20Derbali"> Walid Derbali</a>, <a href="https://publications.waset.org/abstracts/search?q=Arafet%20Manaa"> Arafet Manaa</a>, <a href="https://publications.waset.org/abstracts/search?q=Simone%20Cantamessa"> Simone Cantamessa</a>, <a href="https://publications.waset.org/abstracts/search?q=Graziella%20Berta"> Graziella Berta</a>, <a href="https://publications.waset.org/abstracts/search?q=Chedly%20Abdelly"> Chedly Abdelly</a>, <a href="https://publications.waset.org/abstracts/search?q=Roberto%20Barbato"> Roberto Barbato</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Salinity is one of the most important abiotic affecting plant growth and productivity worldwide. Photosynthesis, together with cell growth, is among the primary processes to be affected by salinity. Here, we report the effects of salinity stress on the primary processes of photosynthesis in a model halophyte Thellungiella Salsuginea. Plants were cultivated in hydroponic system with different NaCl concentrations (0, 100, 200 and 400 mM) during 2 weeks. The obtained results showed an obvious change in the photosynthetic efficiency of photosystem I (PSI) and phostosytem II (PSII), related to NaCl concentration supplemented to the medium and the stress duration considered. With moderate salinity (100 and 200 mM NaCl), no significant variation was observed in photosynthetic parameters of PSI and PSII and Chl fluorescence whatever the time of stress application. Also, the photosynthesis apparatus Fo, Fm and Fv fluorescence, as well as Fv/Fm were not affected by salt stress. While a significant decrease was observed on quantum yields Y(I), Y(II) and electron transport rate ETR(I), ETR(II) under high salt treatment (400 mM NaCl) with prolonged period (15 days). This reduction is quantitatively compensated by a corresponding increase of energy dissipation Y(NPQ) and a progressive decrease in Fv/Fm under salt treatment. The intensity of the OJIP fluorescence transient decreased with increase in NaCl concentration, with a major effect observed during prolonged period of salt stress. Ultrastructural analysis with Light Microscopy and Transmission Electron Microscopy of T. salsuginea chloroplasts showed some cellular changes, such as the shape of the mesophyll cells and number of chloroplast/cell only under higher NaCl concentration. Salt-stress caused the swelling of thylakoids in T. Salsuginea mesophyll with more accumulation of starch as compared to control plant. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fluorescence" title="fluorescence">fluorescence</a>, <a href="https://publications.waset.org/abstracts/search?q=halophyte" title=" halophyte"> halophyte</a>, <a href="https://publications.waset.org/abstracts/search?q=photosynthesis" title=" photosynthesis"> photosynthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=salt%20stress" title=" salt stress"> salt stress</a> </p> <a href="https://publications.waset.org/abstracts/82479/comparative-analysis-of-short-and-long-term-salt-stress-on-the-photosynthetic-apparatus-and-chloroplast-ultrastructure-of-thellungiella-salsuginea" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/82479.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">376</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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