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(PDF) Impact of seawater acidification on pH at the tissue–skeleton interface and calcification in reef corals | Sylvie Tambutté - Academia.edu

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"https://www.academia.edu/login?post_login_redirect_url=https%3A%2F%2Fwww.academia.edu%2F11480029%2FImpact_of_seawater_acidification_on_pH_at_the_tissue_skeleton_interface_and_calcification_in_reef_corals%3Fshow_translation%3Dtrue"; window.loswp.previewableAttachments = [{"id":46682265,"identifier":"Attachment_46682265","shouldShowBulkDownload":false}]; window.loswp.shouldDetectTimezone = true; window.loswp.shouldShowBulkDownload = true; window.loswp.showSignupCaptcha = false window.loswp.willEdgeCache = false; window.loswp.work = {"work":{"id":11480029,"created_at":"2015-03-17T03:04:38.108-07:00","from_world_paper_id":133409717,"updated_at":"2024-11-17T02:07:06.353-08:00","_data":{"grobid_abstract":"Insight into the response of reef corals and other major marine calcifiers to ocean acidification is limited by a lack of knowledge about how seawater pH and carbonate chemistry impact the physiological processes that drive biomineralization. Ocean acidification is proposed to reduce calcification rates in corals by causing declines in internal pH at the calcifying tissue-skeleton interface where biomineralization takes place. Here, we performed an in vivo study on how partial-pressure CO 2 -driven seawater acidification impacts intracellular pH in coral calcifying cells and extracellular pH in the fluid at the tissue-skeleton interface [subcalicoblastic medium (SCM)] in the coral Stylophora pistillata. We also measured calcification in corals grown under the same conditions of seawater acidification by measuring lateral growth of colonies and growth of aragonite crystals under the calcifying tissue. Our findings confirm that seawater acidification decreases pH of the SCM, but this decrease is gradual relative to the surrounding seawater, leading to an increasing pH gradient between the SCM and seawater. Reductions in calcification rate, both at the level of crystals and whole colonies, were only observed in our lowest pH treatment when pH was significantly depressed in the calcifying cells in addition to the SCM. Overall, our findings suggest that reef corals may mitigate the effects of seawater acidification by regulating pH in the SCM, but they also highlight the role of calcifying cell pH homeostasis in determining the response of reef corals to changes in external seawater pH and carbonate chemistry.","publication_date":"2013,,","grobid_abstract_attachment_id":"46682265"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Impact of seawater acidification on pH at the tissue–skeleton interface and calcification in reef corals","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [27960395]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; 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data-entity-id="74228669" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/74228669/Decoupling_between_the_response_of_coral_calcifying_fluid_pH_and_calcification_to_ocean_acidification">Decoupling between the response of coral calcifying fluid pH and calcification to ocean acidification</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="58016911" href="https://independent.academia.edu/steevecomeau">steeve comeau</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Scientific Reports</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Decoupling between the response of coral calcifying fluid pH and calcification to ocean 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class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/74228654/Coral_calcifying_fluid_pH_is_modulated_by_seawater_carbonate_chemistry_not_solely_seawater_pH">Coral calcifying fluid pH is modulated by seawater carbonate chemistry not solely seawater pH</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="58016911" href="https://independent.academia.edu/steevecomeau">steeve comeau</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Proceedings of the Royal Society B: Biological Sciences</p><p class="ds-related-work--abstract ds2-5-body-sm">Reef coral calcification depends on regulation of pH in the internal calcifying fluid (CF) in which the coral skeleton forms. 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Of specific interest are the links between bio-mineralization proteins and the precipitation of new calcium carbonate (CaCO 3), which potentially can provide a better understanding of the biomineralization process. We have assessed the effects of increased CO 2 on the calcification process in cell cultures of the stony coral, Stylophora pistillata, reared in nutrient-enriched artificial seawater at four pCO 2 levels and two glucose concentrations. Dispersed S. pistillata cells grown at low (400 ppmV) and moderate (700 ppmV) pCO 2 re-aggregate into proto-polyps and precipitate CaCO 3. When grown at pCO 2 levels of 1000 ppmV and 2000 ppmV, the cells up-regulate genes for two highly acidic proteins as well as a carbonic anhydrase, but down-regulate long term cadherin protein production and minimize proto-polyp formation, and exhibit a significant decrease in measurable CaCO 3 precipitation. However, cell cultures precipitate CaCO 3 in all treatments, even at slightly undersaturated conditions (X aragonite &lt; 0.95). Glucose addition does not influence either biomineralization gene expression or calcifica-tion rate. Measured d 11 B of the mineral phase, as a proxy of the pH at the calcifying sites, is out of equilibrium with the ambient seawater medium surrounding the cells and proto-polyps, suggesting pH is elevated in the micro-environment of the precipitating mineral. Our results suggest that coral cells possess molecular mechanisms to help compensate for the effects of ocean acidification within the bounds projected in the coming century.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Molecular and geochemical perspectives on the influence of CO 2 on calcification in coral cell cultures&quot;,&quot;attachmentId&quot;:54026706,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/34093983/Molecular_and_geochemical_perspectives_on_the_influence_of_CO_2_on_calcification_in_coral_cell_cultures&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/34093983/Molecular_and_geochemical_perspectives_on_the_influence_of_CO_2_on_calcification_in_coral_cell_cultures"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="54642267" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/54642267/Coral_calcification_mechanisms_facilitate_adaptive_responses_to_ocean_acidification">Coral calcification mechanisms facilitate adaptive responses to ocean acidification</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="31659913" href="https://independent.academia.edu/MalcolmTMcCulloch">Malcolm T McCulloch</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Proceedings. Biological sciences, 2017</p><p class="ds-related-work--abstract ds2-5-body-sm">Ocean acidification (OA) is a pressing threat to reef-building corals, but it remains poorly understood how coral calcification is inhibited by OA and whether corals could acclimatize and/or adapt to OA. Using a novel geochemical approach, we reconstructed the carbonate chemistry of the calcifying fluid in two coral species using both a pH and dissolved inorganic carbon (DIC) proxy (δB and B/Ca, respectively). To address the potential for adaptive responses, both species were collected from two sites spanning a natural gradient in seawater pH and temperature, and then subjected to three pH levels (8.04, 7.88, 7.71) crossed by two temperatures (control, +1.5°C) for 14 weeks. Corals from the site with naturally lower seawater pH calcified faster and maintained growth better under simulated OA than corals from the higher-pH site. This ability was consistently linked to higher pH yet lower DIC values in the calcifying fluid, suggesting that these differences are the result of long-term ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Coral calcification mechanisms facilitate adaptive responses to ocean acidification&quot;,&quot;attachmentId&quot;:70909073,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/54642267/Coral_calcification_mechanisms_facilitate_adaptive_responses_to_ocean_acidification&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/54642267/Coral_calcification_mechanisms_facilitate_adaptive_responses_to_ocean_acidification"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="11480045" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/11480045/Coral_calcifying_fluid_pH_dictates_response_to_ocean_acidification">Coral calcifying fluid pH dictates response to ocean acidification</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="27960395" href="https://independent.academia.edu/SylvieTambutt%C3%A9">Sylvie Tambutté</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link 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translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="86559283" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/86559283/The_effect_of_ocean_acidification_on_tropical_coral_calcification_Insights_from_calcification_fluid_DIC_chemistry">The effect of ocean acidification on tropical coral calcification: Insights from calcification fluid DIC chemistry</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="38397522" href="https://independent.academia.edu/AdrianFinch">Adrian Finch</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Chemical Geology, 2018</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" 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class="ds-related-work--metadata ds2-5-body-xs">Geochimica et Cosmochimica Acta, 2013</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Sr/Ca sensitivity to aragonite saturation state in cultured subsamples from a single colony of coral: Mechanism of biomineralization during ocean acidification&quot;,&quot;attachmentId&quot;:50214707,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/29761481/Sr_Ca_sensitivity_to_aragonite_saturation_state_in_cultured_subsamples_from_a_single_colony_of_coral_Mechanism_of_biomineralization_during_ocean_acidification&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a 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Pelejero</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="42644114" href="https://csic.academia.edu/MartaRibes">Marta Ribes</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Experimental Marine Biology and Ecology, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Calcification reduction and recovery in native and non-native Mediterranean corals in response to ocean acidification&quot;,&quot;attachmentId&quot;:42122551,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/21580448/Calcification_reduction_and_recovery_in_native_and_non_native_Mediterranean_corals_in_response_to_ocean_acidification&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" 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data-entity-id="26341751" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/26341751/Measuring_gross_and_net_calcification_of_a_reef_coral_under_ocean_acidification_conditions_methodological_considerations">Measuring gross and net calcification of a reef coral under ocean acidification conditions: methodological considerations</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="4659422" href="https://biu.academia.edu/MaozFine">Maoz Fine</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Measuring gross and net calcification of a reef coral under ocean acidification conditions: methodological 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href="https://uwa.academia.edu/JamesLFalter">James L Falter</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Nature Climate Change, 2012</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Coral resilience to ocean acidification and global warming through pH up-regulation&quot;,&quot;attachmentId&quot;:37280190,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/11906460/Coral_resilience_to_ocean_acidification_and_global_warming_through_pH_up_regulation&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" 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