Yeast Display based Binder Discovery Services

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This method leverages the eukaryotic system of yeast, which ensures proper protein folding, modification, and translocation before surface display. Capitalizing on these benefits, Creative Biolabs has developed the Ultraff™ yeast display platform, a unique and advanced system for constructing yeast display libraries, screening, and antibody affinity maturation, among other applications. </p> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Services</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Platform</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Advantages</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Cases</span> </div> <script> window.addEventListener('load',function(){ page_tab_margin() page_tab_css(); })</script> <h2 class="h2_tit page_tab_margin" id="tab1"> Yeast Display based Binder Discovery </h2> <p> With extensive experience accumulated over decades, Creative Biolabs has established the unique Ultraff™ Yeast Display System, a highly robust platform that utilizes the expression of recombinant proteins on the surface of yeast cells to isolate and engineer high-affinity binders, such as antibodies or peptides. This advanced system allows us to selectively isolate yeast cells displaying binders with desired affinities and specificities, ensuring the highest quality outcomes. We have successfully discovered and optimized monoclonal antibodies with picomolar (pM) affinity, the highest ever achieved through <em>in vitro</em> recombinant antibody production technology. We collaborate closely with you to understand your specific needs and tailor our services and products to meet your goals effectively. </p> <p> <strong>Comprehensive Services</strong> </p> <p> Creative Biolabs offers a robust yeast display system designed to optimize the discovery and development of proteins or antibodies. At Creative Biolabs, we offer a suite of services tailored to support your yeast display-based binder discovery projects: </p> <div class="row textboxstyle-area"> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a href="/yeast-display-library-construction-services.html"><span>Custom Yeast Display Library Construction</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> Diverse genetic materials. </li> <li> Custom library design. </li> <li> Efficient transformation protocol. </li> <li> High diversity libraries. </li> </ul> </div> </a> </div> </div> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a href="/yeast-display-library-screening-services.html"><span>Yeast Display Library Screening</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> FACS-based screening. </li> <li> Selective isolation high-affinity binders. </li> </ul> </div> </a> </div> </div> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a href="/yeast-display-based-antibody-affinity-maturation-service.html"><span>Yeast Display-Based Antibody Affinity Maturation</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> Enhancement of the affinity and specificity. </li> <li> Engineering and optimizing with improved performance. </li> </ul> </div> </a> </div> </div> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a href="/yeast-display-based-t-cell-receptor-engineering-service.html"><span>Yeast Display-Based T Cell Receptor Engineering</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> Specialized T cell receptors for various therapeutic applications. </li> <li> Custom Solutions to unique project requirements. </li> </ul> </div> </a> </div> </div> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a href="/human-monoclonal-antibody-identification-through-phage-yeast-display-combination-service.html"><span>Human Monoclonal Antibody Identification Through Phage & Yeast Display Combination</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> Dual display platforms to maximize antibody potential. </li> <li> Synergistic Approach to isolate antibodies with high purity and yield. </li> </ul> </div> </a> </div> </div> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a><span>Yeast Display-Based Protein Optimization and Engineering</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> Protein Enhancement </li> <li> Tailored Solutions </li> </ul> </div> </a> </div> </div> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a><span>Yeast Display-Based Antibody Discovery Service</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> Robust Discovery Platform </li> <li> Comprehensive Screening </li> </ul> </div> </a> </div> </div> <div class="col-md-6"> <div class="textboxstyle-area-yellow"> <a><span>Yeast Display-Based Peptide Discovery</span> <div class="box-radius_titarea"> <ul class="ullist"> <li> Diverse Peptide Libraries </li> <li> High-Throughput Identification </li> </ul> </div> </a> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab2"> Ultraff™ Yeast Display Platform </h2> <p> Yeast surface display, often referred to as yeast display, is a powerful tool for protein engineering with diverse applications in biotechnology and biomedicine. It is widely utilized for <em>in vitro</em> protein evolution. This technique serves as an alternative for isolating and engineering antibody fragments, such as Fab, scFv, and sdAb, from both immune and non-immune libraries. Yeast display has been successfully applied to isolate recombinant antibodies with binding specificity to various proteins, peptides, small molecules, and T cell receptors. </p> <p> <strong>Key Features of Ultraff™ Yeast Display</strong> </p> <ul class="ullist"> <li> Genotype-Phenotype Link<br /> Much like phage display, yeast display establishes a direct connection between genotype and phenotype. The gene of interest is housed within a plasmid inside the yeast cells, and the encoded antibody is expressed on the cell surface. </li> <li> High Display Level<br /> Each yeast cell displays 10,000 to 100,000 copies of the protein of interest. </li> <li> Quantitative Screening<br /> Fluorescence-activated cell sorting (FACS) is used to quantify surface expression and avidity, measuring both antigen binding strength and antibody expression levels. </li> <li> Precise Selection<br /> FACS enables precise control of selection parameters, including binding population percentage, signal normalization, and desired binding affinities, through flow cytometry. </li> <li> Enrichment Rounds<br /> Typically, 3-5 rounds of enrichment are conducted to isolate target antigen-binding clones. </li> <li> Specificity and Reproducibility Validation<br /> Antibodies displayed by single yeast colonies are evaluated for specificity and reproducibility. If functional, the antibody gene is sequenced for validation. </li> </ul> <br /> <p> <strong>Applications of Ultraff™ Yeast Display</strong> </p> <div class="row"> <div class="col-md-4 col-sm-6"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-11.png" width="120" height="120" alt="" /><br /> Therapeutic Antibody Discovery</strong> </div> <div class="col-md-4 col-sm-6"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-12.png" width="120" height="120" alt="" /><br /> Monoclonal Antibody Discovery</strong> </div> <div class="col-md-4 col-sm-6"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-13.png" width="120" height="120" alt="" /><br /> Antibody Affinity Maturation</strong> </div> <div class="col-md-4 col-sm-6"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-14.png" width="120" height="120" alt="" /><br /> Antibody Humanization</strong> </div> <div class="col-md-4 col-sm-6"> <strong class="featurestit"> <img loading="lazy" src="../static/img/single-domain-antibody-sdAb-discovery-services-15.png" width="120" height="120" alt="" /><br /> Protein Interaction Studies</strong> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab3"> Benefits of Choosing Creative Biolabs </h2> <div class="row"> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"><img loading="lazy" src="../static/img/1-1-2-yeast-display-based-binder-discovery-3.jpg" width="120" height="120" alt="" /><br /> Expertise</strong> <div class="featurescont"> Our team brings decades of experience in yeast display technology and binder discovery, offering unparalleled knowledge and skills. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"><img loading="lazy" src="../static/img/1-1-2-yeast-display-based-binder-discovery-4.jpg" width="120" height="120" alt="" /><br /> Innovation</strong> <div class="featurescont"> We use cutting-edge techniques and proprietary platforms, ensuring the highest quality and efficiency in our services. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"><img loading="lazy" src="../static/img/1-1-2-yeast-display-based-binder-discovery-5.jpg" width="120" height="120" alt="" /><br /> Customization</strong> <div class="featurescont"> We offer tailored solutions to meet your specific project needs, providing both flexibility and precision. </div> </div> </div> <div class="col-md-4 col-sm-6"> <div class="featuresunit"> <strong class="featurestit"><img loading="lazy" src="../static/img/1-1-2-yeast-display-based-binder-discovery-6.jpg" width="120" height="120" alt="" /><br /> Comprehensive Support</strong> <div class="featurescont"> From initial consultation to final delivery, our dedicated team provides complete support, ensuring your project's success. </div> </div> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab4"> Cases </h2> <h3 class="h3_tit"> <strong>Yeast Surface Display and Antigen Binding Test</strong> </h3> <p class="ServiceShowPic"> <img loading="lazy" src="../static/img/1-1-2-yeast-display-based-binder-discovery-2.jpg" alt="Fig. 2 A FC result of yeast surface display and antigen binding test. (Creative Biolabs Original)" width="824" height="400" /> </p> <p> <strong>Results</strong> </p> <ol class="ollist" style="margin-bottom:15px;"> <li> The positive staining results of anti-myc/HA tag antibodies revealed that the display of scFv antibody on yeast cell surface is successful. </li> <li> The positive binding results of biotin-Ag revealed that the scFv antibody is displayed in the correct conformation on the yeast cell surface. </li> </ol> <p> Harness the power of our yeast display services to elevate your protein engineering projects. <span class="inquiry-button">Contact us</span> today to discuss your specific needs and discover how our customized solutions can advance your research or biotech initiatives. </p><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.</p> <div class="related-sections"> <b>Related Sections</b> <b>Services</b> <ul class="related-sections-ul"> <li><a href="/phage-display-service.html" >Phage Display based Binder Discovery Services</a></li> <li><a href="/bacterial-display-platform.html" >Bacterial Display based Binder Discovery Services</a></li> <li><a href="/ribosome-display-services.html" >Ribosome Display based Binder Discovery Services</a></li> <li><a href="/mammalian-cell-display-service.html" >Mammalian Display based Binder Discovery Services</a></li> </ul> <b>Products</b> <ul class="related-sections-ul"> <li><a href="category-premade-yeast-display-library-ready-to-use-kits-6.htm" >Premade Yeast Display Library Ready-To-Use Kits</a></li> </ul> <b>Platform</b> <ul class="related-sections-ul"> <li><a href="/yeast-display-platform.html" >Yeast Display Platform</a></li> </ul> </div> </div> <div class="col-md-3"> <a name="OnlineInquiry"></a> <div 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