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mw-list-item"><a href="/w/index.php?title=%E7%89%B9%E5%88%A5:%E3%82%A2%E3%82%AB%E3%82%A6%E3%83%B3%E3%83%88%E4%BD%9C%E6%88%90&returnto=%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5&returntoquery=printable%3Dyes" title="アカウントを作成してログインすることをお勧めしますが、必須ではありません"><span class="vector-icon mw-ui-icon-userAdd mw-ui-icon-wikimedia-userAdd"></span> <span>アカウント作成</span></a></li><li id="pt-login" class="user-links-collapsible-item mw-list-item"><a href="/w/index.php?title=%E7%89%B9%E5%88%A5:%E3%83%AD%E3%82%B0%E3%82%A4%E3%83%B3&returnto=%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5&returntoquery=printable%3Dyes" title="ログインすることを推奨します。ただし、必須ではありません。 [o]" accesskey="o"><span class="vector-icon mw-ui-icon-logIn mw-ui-icon-wikimedia-logIn"></span> <span>ログイン</span></a></li> </ul> </div> </div> <div id="p-user-menu-anon-editor" class="vector-menu mw-portlet mw-portlet-user-menu-anon-editor" > <div class="vector-menu-heading"> ログアウトした編集者のページ <a 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id="mw-panel" class="vector-main-menu-landmark" aria-label="サイト"> <div id="vector-main-menu-pinned-container" class="vector-pinned-container"> </div> </nav> </div> </div> <div class="vector-sticky-pinned-container"> <nav id="mw-panel-toc" aria-label="目次" data-event-name="ui.sidebar-toc" class="mw-table-of-contents-container vector-toc-landmark"> <div id="vector-toc-pinned-container" class="vector-pinned-container"> <div id="vector-toc" class="vector-toc vector-pinnable-element"> <div class="vector-pinnable-header vector-toc-pinnable-header vector-pinnable-header-pinned" data-feature-name="toc-pinned" data-pinnable-element-id="vector-toc" > <h2 class="vector-pinnable-header-label">目次</h2> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-pin-button" data-event-name="pinnable-header.vector-toc.pin">サイドバーに移動</button> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-unpin-button" data-event-name="pinnable-header.vector-toc.unpin">非表示</button> </div> <ul class="vector-toc-contents" id="mw-panel-toc-list"> <li id="toc-mw-content-text" class="vector-toc-list-item vector-toc-level-1"> <a href="#" class="vector-toc-link"> <div class="vector-toc-text">ページ先頭</div> </a> </li> <li id="toc-遺伝子導入の手段" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#遺伝子導入の手段"> <div class="vector-toc-text"> <span class="vector-toc-numb">1</span> <span>遺伝子導入の手段</span> </div> </a> <ul id="toc-遺伝子導入の手段-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-遺伝子導入の方法" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#遺伝子導入の方法"> <div class="vector-toc-text"> <span class="vector-toc-numb">2</span> <span>遺伝子導入の方法</span> </div> </a> <ul id="toc-遺伝子導入の方法-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-遺伝子導入の応用" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#遺伝子導入の応用"> <div class="vector-toc-text"> <span class="vector-toc-numb">3</span> <span>遺伝子導入の応用</span> </div> </a> <ul id="toc-遺伝子導入の応用-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-脚注・出典" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#脚注・出典"> <div class="vector-toc-text"> <span class="vector-toc-numb">4</span> <span>脚注・出典</span> </div> </a> <ul id="toc-脚注・出典-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-外部リンク" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#外部リンク"> <div class="vector-toc-text"> <span class="vector-toc-numb">5</span> <span>外部リンク</span> </div> </a> <ul id="toc-外部リンク-sublist" class="vector-toc-list"> </ul> </li> </ul> </div> </div> </nav> </div> </div> <div class="mw-content-container"> <main id="content" class="mw-body"> <header class="mw-body-header vector-page-titlebar"> <nav aria-label="目次" class="vector-toc-landmark"> <div id="vector-page-titlebar-toc" class="vector-dropdown vector-page-titlebar-toc vector-button-flush-left" > <input type="checkbox" id="vector-page-titlebar-toc-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-page-titlebar-toc" class="vector-dropdown-checkbox " aria-label="目次の表示・非表示を切り替え" > <label id="vector-page-titlebar-toc-label" for="vector-page-titlebar-toc-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--icon-only " aria-hidden="true" ><span class="vector-icon mw-ui-icon-listBullet mw-ui-icon-wikimedia-listBullet"></span> <span class="vector-dropdown-label-text">目次の表示・非表示を切り替え</span> </label> <div class="vector-dropdown-content"> <div id="vector-page-titlebar-toc-unpinned-container" class="vector-unpinned-container"> </div> </div> </div> </nav> <h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">遺伝子導入</span></h1> <div id="p-lang-btn" class="vector-dropdown mw-portlet mw-portlet-lang" > <input type="checkbox" id="p-lang-btn-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-p-lang-btn" class="vector-dropdown-checkbox mw-interlanguage-selector" aria-label="特定の記事の別の言語版に移動します。利用可能な言語13件" > <label id="p-lang-btn-label" for="p-lang-btn-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--action-progressive mw-portlet-lang-heading-13" aria-hidden="true" ><span class="vector-icon mw-ui-icon-language-progressive mw-ui-icon-wikimedia-language-progressive"></span> <span class="vector-dropdown-label-text">13の言語版</span> </label> <div class="vector-dropdown-content"> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li class="interlanguage-link interwiki-ar mw-list-item"><a href="https://ar.wikipedia.org/wiki/%D9%86%D9%82%D9%84_%D9%88%D8%B1%D8%A7%D8%AB%D9%8A" title="アラビア語: نقل وراثي" lang="ar" hreflang="ar" data-title="نقل وراثي" data-language-autonym="العربية" data-language-local-name="アラビア語" class="interlanguage-link-target"><span>العربية</span></a></li><li class="interlanguage-link interwiki-ca mw-list-item"><a href="https://ca.wikipedia.org/wiki/Transg%C3%A8nesi" title="カタロニア語: Transgènesi" lang="ca" hreflang="ca" data-title="Transgènesi" data-language-autonym="Català" data-language-local-name="カタロニア語" class="interlanguage-link-target"><span>Català</span></a></li><li class="interlanguage-link interwiki-en badge-Q70893996 mw-list-item" title=""><a href="https://en.wikipedia.org/wiki/Transgenesis" title="英語: Transgenesis" lang="en" hreflang="en" data-title="Transgenesis" data-language-autonym="English" data-language-local-name="英語" class="interlanguage-link-target"><span>English</span></a></li><li class="interlanguage-link interwiki-es mw-list-item"><a href="https://es.wikipedia.org/wiki/Transg%C3%A9nesis" title="スペイン語: Transgénesis" lang="es" hreflang="es" data-title="Transgénesis" data-language-autonym="Español" data-language-local-name="スペイン語" class="interlanguage-link-target"><span>Español</span></a></li><li class="interlanguage-link interwiki-fa mw-list-item"><a href="https://fa.wikipedia.org/wiki/%D8%AA%D8%B1%D8%A7%D8%B1%DB%8C%D8%AE%D8%AA%D9%87" title="ペルシア語: تراریخته" lang="fa" hreflang="fa" data-title="تراریخته" data-language-autonym="فارسی" data-language-local-name="ペルシア語" class="interlanguage-link-target"><span>فارسی</span></a></li><li class="interlanguage-link interwiki-fr mw-list-item"><a href="https://fr.wikipedia.org/wiki/Transg%C3%A9n%C3%A8se" title="フランス語: Transgénèse" lang="fr" hreflang="fr" data-title="Transgénèse" data-language-autonym="Français" data-language-local-name="フランス語" class="interlanguage-link-target"><span>Français</span></a></li><li class="interlanguage-link interwiki-ga mw-list-item"><a href="https://ga.wikipedia.org/wiki/Trasgh%C3%A9ineach" title="アイルランド語: Trasghéineach" lang="ga" hreflang="ga" data-title="Trasghéineach" data-language-autonym="Gaeilge" data-language-local-name="アイルランド語" class="interlanguage-link-target"><span>Gaeilge</span></a></li><li class="interlanguage-link interwiki-it mw-list-item"><a href="https://it.wikipedia.org/wiki/Transgenesi" title="イタリア語: Transgenesi" lang="it" hreflang="it" data-title="Transgenesi" data-language-autonym="Italiano" data-language-local-name="イタリア語" class="interlanguage-link-target"><span>Italiano</span></a></li><li class="interlanguage-link interwiki-lt mw-list-item"><a href="https://lt.wikipedia.org/wiki/Transgenez%C4%97" title="リトアニア語: Transgenezė" lang="lt" hreflang="lt" data-title="Transgenezė" data-language-autonym="Lietuvių" data-language-local-name="リトアニア語" class="interlanguage-link-target"><span>Lietuvių</span></a></li><li class="interlanguage-link interwiki-oc mw-list-item"><a href="https://oc.wikipedia.org/wiki/Transgen%C3%A8si" title="オック語: Transgenèsi" lang="oc" hreflang="oc" data-title="Transgenèsi" data-language-autonym="Occitan" data-language-local-name="オック語" class="interlanguage-link-target"><span>Occitan</span></a></li><li class="interlanguage-link interwiki-pt mw-list-item"><a href="https://pt.wikipedia.org/wiki/Transg%C3%AAnese" title="ポルトガル語: Transgênese" lang="pt" hreflang="pt" data-title="Transgênese" data-language-autonym="Português" data-language-local-name="ポルトガル語" class="interlanguage-link-target"><span>Português</span></a></li><li class="interlanguage-link interwiki-ru mw-list-item"><a href="https://ru.wikipedia.org/wiki/%D0%A2%D1%80%D0%B0%D0%BD%D1%81%D0%B3%D0%B5%D0%BD%D0%B5%D0%B7" title="ロシア語: Трансгенез" lang="ru" hreflang="ru" data-title="Трансгенез" data-language-autonym="Русский" data-language-local-name="ロシア語" class="interlanguage-link-target"><span>Русский</span></a></li><li class="interlanguage-link interwiki-scn mw-list-item"><a href="https://scn.wikipedia.org/wiki/Transg%C3%A8nisi" title="シチリア語: Transgènisi" lang="scn" hreflang="scn" data-title="Transgènisi" data-language-autonym="Sicilianu" data-language-local-name="シチリア語" class="interlanguage-link-target"><span>Sicilianu</span></a></li> </ul> <div class="after-portlet after-portlet-lang"><span class="wb-langlinks-edit wb-langlinks-link"><a href="https://www.wikidata.org/wiki/Special:EntityPage/Q2305545#sitelinks-wikipedia" title="言語間リンクを編集" class="wbc-editpage">リンクを編集</a></span></div> </div> </div> </div> </header> <div class="vector-page-toolbar"> <div class="vector-page-toolbar-container"> <div id="left-navigation"> <nav aria-label="名前空間"> <div id="p-associated-pages" class="vector-menu vector-menu-tabs mw-portlet mw-portlet-associated-pages" > <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="ca-nstab-main" class="selected vector-tab-noicon mw-list-item"><a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5" title="本文を閲覧 [c]" accesskey="c"><span>ページ</span></a></li><li id="ca-talk" class="new vector-tab-noicon mw-list-item"><a href="/w/index.php?title=%E3%83%8E%E3%83%BC%E3%83%88:%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5&action=edit&redlink=1" rel="discussion" class="new" title="「本文ページについての議論」 (存在しないページ) [t]" accesskey="t"><span>ノート</span></a></li> </ul> </div> </div> <div id="vector-variants-dropdown" class="vector-dropdown emptyPortlet" > <input type="checkbox" id="vector-variants-dropdown-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-variants-dropdown" class="vector-dropdown-checkbox " aria-label="別の言語に切り替える" > <label id="vector-variants-dropdown-label" for="vector-variants-dropdown-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet" aria-hidden="true" ><span class="vector-dropdown-label-text">日本語</span> </label> <div class="vector-dropdown-content"> <div id="p-variants" class="vector-menu mw-portlet mw-portlet-variants emptyPortlet" > <div class="vector-menu-content"> <ul class="vector-menu-content-list"> </ul> </div> </div> </div> </div> </nav> </div> <div id="right-navigation" class="vector-collapsible"> <nav aria-label="表示"> <div id="p-views" class="vector-menu vector-menu-tabs mw-portlet mw-portlet-views" > <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="ca-view" class="selected vector-tab-noicon mw-list-item"><a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5"><span>閲覧</span></a></li><li id="ca-edit" class="vector-tab-noicon mw-list-item"><a href="/w/index.php?title=%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5&action=edit" title="このページのソースコードを編集する [e]" accesskey="e"><span>編集</span></a></li><li id="ca-history" class="vector-tab-noicon mw-list-item"><a href="/w/index.php?title=%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5&action=history" title="このページの過去の版 [h]" accesskey="h"><span>履歴表示</span></a></li> </ul> </div> </div> </nav> <nav class="vector-page-tools-landmark" aria-label="ページツール"> <div id="vector-page-tools-dropdown" class="vector-dropdown vector-page-tools-dropdown" > <input type="checkbox" id="vector-page-tools-dropdown-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-page-tools-dropdown" class="vector-dropdown-checkbox " aria-label="ツール" > <label id="vector-page-tools-dropdown-label" for="vector-page-tools-dropdown-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet" aria-hidden="true" ><span class="vector-dropdown-label-text">ツール</span> </label> <div class="vector-dropdown-content"> <div id="vector-page-tools-unpinned-container" class="vector-unpinned-container"> <div id="vector-page-tools" class="vector-page-tools vector-pinnable-element"> <div class="vector-pinnable-header vector-page-tools-pinnable-header vector-pinnable-header-unpinned" data-feature-name="page-tools-pinned" data-pinnable-element-id="vector-page-tools" data-pinned-container-id="vector-page-tools-pinned-container" data-unpinned-container-id="vector-page-tools-unpinned-container" > <div class="vector-pinnable-header-label">ツール</div> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-pin-button" data-event-name="pinnable-header.vector-page-tools.pin">サイドバーに移動</button> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-unpin-button" data-event-name="pinnable-header.vector-page-tools.unpin">非表示</button> </div> <div id="p-cactions" class="vector-menu mw-portlet mw-portlet-cactions emptyPortlet vector-has-collapsible-items" title="その他の操作" > <div class="vector-menu-heading"> 操作 </div> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="ca-more-view" class="selected vector-more-collapsible-item mw-list-item"><a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5"><span>閲覧</span></a></li><li id="ca-more-edit" class="vector-more-collapsible-item mw-list-item"><a href="/w/index.php?title=%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5&action=edit" title="このページのソースコードを編集する [e]" accesskey="e"><span>編集</span></a></li><li id="ca-more-history" class="vector-more-collapsible-item mw-list-item"><a href="/w/index.php?title=%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5&action=history"><span>履歴表示</span></a></li> </ul> </div> </div> <div id="p-tb" class="vector-menu mw-portlet mw-portlet-tb" > <div class="vector-menu-heading"> 全般 </div> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="t-whatlinkshere" class="mw-list-item"><a href="/wiki/%E7%89%B9%E5%88%A5:%E3%83%AA%E3%83%B3%E3%82%AF%E5%85%83/%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B0%8E%E5%85%A5" title="ここにリンクしている全ウィキページの一覧 [j]" accesskey="j"><span>リンク元</span></a></li><li id="t-recentchangeslinked" class="mw-list-item"><a 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vector-appearance-pinnable-header vector-pinnable-header-pinned" data-feature-name="appearance-pinned" data-pinnable-element-id="vector-appearance" data-pinned-container-id="vector-appearance-pinned-container" data-unpinned-container-id="vector-appearance-unpinned-container" > <div class="vector-pinnable-header-label">表示</div> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-pin-button" data-event-name="pinnable-header.vector-appearance.pin">サイドバーに移動</button> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-unpin-button" data-event-name="pinnable-header.vector-appearance.unpin">非表示</button> </div> </div> </div> </nav> </div> </div> <div id="bodyContent" class="vector-body" aria-labelledby="firstHeading" data-mw-ve-target-container> <div class="vector-body-before-content"> <div class="mw-indicators"> </div> <div id="siteSub" class="noprint">出典: フリー百科事典『ウィキペディア（Wikipedia）』</div> </div> <div id="contentSub"><div id="mw-content-subtitle"></div></div> <div id="mw-content-text" class="mw-body-content"><div class="cdx-message cdx-message--block cdx-message--warning"><span class="cdx-message__icon"></span><div class="cdx-message__content">印刷用ページはサポート対象外です。表示エラーが発生する可能性があります。ブラウザーのブックマークを更新し、印刷にはブラウザーの印刷機能を使用してください。</div></div><div class="mw-content-ltr mw-parser-output" lang="ja" dir="ltr"><p><b>遺伝子導入</b>（いでんしどうにゅう、transgenesis）は、外来<a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90" title="遺伝子">遺伝子</a>を<a href="/wiki/%E7%94%9F%E7%89%A9" title="生物">生物</a>に導入する過程を指し、外来遺伝子の導入によって、その生物の<a href="/wiki/%E5%BD%A2%E8%B3%AA" title="形質">形質</a>が変わり、新たな能力を獲得する場合がある。 </p><p><a href="/wiki/%E7%B4%B0%E8%8F%8C" title="細菌">細菌</a>、<a href="/wiki/%E9%85%B5%E6%AF%8D" title="酵母">酵母</a>や<a href="/wiki/%E6%A4%8D%E7%89%A9" title="植物">植物</a><a href="/wiki/%E7%B4%B0%E8%83%9E" title="細胞">細胞</a>への遺伝子導入は<b><a href="/wiki/%E5%BD%A2%E8%B3%AA%E8%BB%A2%E6%8F%9B" title="形質転換">形質転換</a></b>（transformation）、<a href="/wiki/%E5%8B%95%E7%89%A9" title="動物">動物</a>細胞への遺伝子導入は<b><a href="/wiki/%E3%83%88%E3%83%A9%E3%83%B3%E3%82%B9%E3%83%95%E3%82%A7%E3%82%AF%E3%82%B7%E3%83%A7%E3%83%B3" title="トランスフェクション">トランスフェクション</a></b>（transfection）、<a href="/wiki/%E3%83%95%E3%82%A1%E3%83%BC%E3%82%B8" title="ファージ">ファージ</a>や<a href="/wiki/%E3%82%A6%E3%82%A4%E3%83%AB%E3%82%B9" title="ウイルス">ウイルス</a>を用いた遺伝子導入は<b><a href="/wiki/%E5%BD%A2%E8%B3%AA%E5%B0%8E%E5%85%A5" title="形質導入">形質導入</a></b>（transduction）と一般的に呼ばれる。 </p> <meta property="mw:PageProp/toc" /> <div class="mw-heading mw-heading2"><h2 id="遺伝子導入の手段"><span id=".E9.81.BA.E4.BC.9D.E5.AD.90.E5.B0.8E.E5.85.A5.E3.81.AE.E6.89.8B.E6.AE.B5"></span>遺伝子導入の手段</h2></div> <p>遺伝子導入を促進する生物学的、化学的または物理的手法があり<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>、<a href="/wiki/%E7%B4%B0%E8%83%9E%E6%A0%AA" class="mw-redirect" title="細胞株">細胞株</a>、初代培養系、動物、植物や細菌への遺伝子導入に利用されている。生物学的手法にはウイルスを用いた方法が広く行われている<sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-3" class="reference"><a href="#cite_note-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup>。化学的方法には<a href="/wiki/%E3%83%AA%E3%83%9D%E3%83%95%E3%82%A7%E3%82%AF%E3%82%B7%E3%83%A7%E3%83%B3" title="リポフェクション">リポフェクション</a>法やポリマーやペプチドを使用する方法<sup id="cite_ref-4" class="reference"><a href="#cite_note-4"><span class="cite-bracket">[</span>4<span class="cite-bracket">]</span></a></sup>等がある。物理的手法には<a href="/wiki/%E9%9B%BB%E6%B0%97%E7%A9%BF%E5%AD%94%E6%B3%95" title="電気穿孔法">電気穿孔法</a>や<a href="/wiki/%E3%83%91%E3%83%BC%E3%83%86%E3%82%A3%E3%82%AF%E3%83%AB%E3%83%BB%E3%82%AC%E3%83%B3%E6%B3%95" title="パーティクル・ガン法">パーティクル・ガン法</a>や超音波を使用する方法（sonoporation）<sup id="cite_ref-5" class="reference"><a href="#cite_note-5"><span class="cite-bracket">[</span>5<span class="cite-bracket">]</span></a></sup>等がある。また細菌の場合、<a href="/wiki/%E5%A1%A9%E5%8C%96%E3%82%AB%E3%83%AB%E3%82%B7%E3%82%A6%E3%83%A0" title="塩化カルシウム">塩化カルシウム</a>存在下で<a href="/wiki/%E3%82%B3%E3%83%B3%E3%83%94%E3%83%86%E3%83%B3%E3%83%88%E3%82%BB%E3%83%AB" title="コンピテントセル">コンピテントセル</a>化した菌にヒートショックで <a href="/wiki/DNA" class="mw-redirect" title="DNA">DNA</a> を形質転換する方法が広く行われている。 </p> <div class="mw-heading mw-heading2"><h2 id="遺伝子導入の方法"><span id=".E9.81.BA.E4.BC.9D.E5.AD.90.E5.B0.8E.E5.85.A5.E3.81.AE.E6.96.B9.E6.B3.95"></span>遺伝子導入の方法</h2></div> <p>遺伝子導入が一過的なものか<a href="/wiki/%E3%82%B2%E3%83%8E%E3%83%A0" title="ゲノム">ゲノム</a>内に組み込む安定的なものかの方法の違いがあり<sup id="cite_ref-6" class="reference"><a href="#cite_note-6"><span class="cite-bracket">[</span>6<span class="cite-bracket">]</span></a></sup>、<a href="/wiki/%E3%83%88%E3%83%A9%E3%83%B3%E3%82%B9%E3%83%9D%E3%82%BE%E3%83%B3" title="トランスポゾン">トランスポゾン</a>を用いてゲノムに挿入する方法<sup id="cite_ref-7" class="reference"><a href="#cite_note-7"><span class="cite-bracket">[</span>7<span class="cite-bracket">]</span></a></sup>やリコンビナーゼを用いて特定のゲノムの位置に挿入する方法<sup id="cite_ref-8" class="reference"><a href="#cite_note-8"><span class="cite-bracket">[</span>8<span class="cite-bracket">]</span></a></sup>がある。 </p> <div class="mw-heading mw-heading2"><h2 id="遺伝子導入の応用"><span id=".E9.81.BA.E4.BC.9D.E5.AD.90.E5.B0.8E.E5.85.A5.E3.81.AE.E5.BF.9C.E7.94.A8"></span>遺伝子導入の応用</h2></div> <p>遺伝子導入は<a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E5%B7%A5%E5%AD%A6" title="遺伝子工学">遺伝子工学</a>の<a href="/wiki/%E5%9F%BA%E7%A4%8E" title="基礎">基礎</a>となる技術である。この技術は<a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E7%B5%84%E3%81%BF%E6%8F%9B%E3%81%88%E4%BD%9C%E7%89%A9" title="遺伝子組み換え作物">遺伝子組み換え作物</a>や<a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E6%B2%BB%E7%99%82" title="遺伝子治療">遺伝子治療</a>等に応用されている。また遺伝子導入は<a href="/wiki/%E3%82%B2%E3%83%8E%E3%83%A0%E8%A7%A3%E6%9E%90" class="mw-redirect" title="ゲノム解析">ゲノム解析</a>、<a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E7%A0%B4%E5%A3%8A" title="遺伝子破壊">遺伝子破壊</a>や<a href="/wiki/%E9%81%BA%E4%BC%9D%E5%AD%90%E3%83%8E%E3%83%83%E3%82%AF%E3%83%80%E3%82%A6%E3%83%B3" title="遺伝子ノックダウン">遺伝子ノックダウン</a>技術の基礎になっており、<a href="/wiki/%E4%BA%BA%E5%B7%A5%E5%A4%9A%E8%83%BD%E6%80%A7%E5%B9%B9%E7%B4%B0%E8%83%9E" title="人工多能性幹細胞">iPS 細胞</a>作成に必要な基礎技術である。 </p> <div class="mw-heading mw-heading2"><h2 id="脚注・出典"><span id=".E8.84.9A.E6.B3.A8.E3.83.BB.E5.87.BA.E5.85.B8"></span>脚注・出典</h2></div> <div class="noprint" style="float:right; font-size:90%;">[<a href="/wiki/Help:%E8%84%9A%E6%B3%A8/%E8%AA%AD%E8%80%85%E5%90%91%E3%81%91" title="Help:脚注/読者向け"><span title="この欄の操作法">脚注の使い方</span></a>]</div> <ol class="references"> <li id="cite_note-1"><b><a href="#cite_ref-1">^</a></b> <span class="reference-text">Kim TK, Eberwine JH. 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