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LoopSeq</div><div class="subnav__links" data-ref="subnav-links"><a href="/products/aviti" class="subnav__link" > AVITI </a><a href="/products/cloudbreak" class="subnav__link" > Cloudbreak </a><a href="/products/adept" class="subnav__link" > Adept </a><a href="/products/elevate" class="subnav__link" > Elevate </a><a href="/products/loopseq" class="subnav__link -is-active" > LoopSeq </a><a href="/products/elembio-cloud" class="subnav__link" > ElemBio Cloud </a></div></div></nav><main class="main" role="main"><div class="product -purple"><section class="product-hero"><div class="container"><div class="product-hero__row"><div class="product-hero__content"><ol class="breadcrumbs"><li> Products </li><li> LoopSeq </li></ol><script type="application/ld+json"> { "@context": "http://schema.org", "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Products" }, { "@type": "ListItem", "position": 2, "name": "LoopSeq" } ] } </script><h1 class="product-hero__heading">LoopSeq™ for AVITI™</h1><div class="product-hero__intro"><p>LoopSeq for AVITI prepares libraries for long-read sequencing on the short-read Element AVITI System, a portfolio of capabilities that is exclusive to Element. To support a variety of long-read applications, we offer two versatile library preps:</p><ul><li><strong>16S LoopSeq for AVITI</strong> kits target the identity and relative species abundance of a microbial community, delivering high-accuracy results that span entire molecules.</li><li><strong>Amplicon LoopSeq for AVITI </strong>kits research the sequence of specific genomes for on-target analysis of genetic variation, suiting a variety of research needs.</li></ul><p>Additional offerings, 16S LoopSeq and Amplicon LoopSeq, enable prepare LoopSeq libraries for sequencing on an alternative short-read platform. LoopSeq Services provide the ease of an experienced services lab.</p></div><div class="product-hero__links"><a href="/contact" class="button -white"> Contact Us for LoopSeq Services </a></div></div><picture class="product-hero__photo"><img src="https://www.elementbiosciences.com/uploads/products/hero/_productHeroPhoto/loopseq-hero-photo.png" width="441" height="391" alt="Loopseq hero photo" ></picture></div></div></section><section class="product-section -justified"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">Long-Read Workflow</h2><div class="product-section__content"><div class="product-text"><h3><strong>Barcode and Amplify</strong></h3><p>Ligation or direct amplification add LoopSeq‑specific sequences. Amplification then adds one unique barcode to each molecule and creates thousands of copies of each molecule and its unique barcode<br /></p></div><figure class="product-photo"><img src="https://www.elementbiosciences.com/uploads/products/photos/_productPhoto/Step-1-Barcode-Amplify.svg" width="20" height="7" alt="Step 1 Barcode Amplify" loading="lazy" ></figure><div class="product-text"><h3><strong>Distribute and Prep</strong></h3><p>Enzymes digest each molecule into fragments and distribute copies of the barcode throughout the fragments, so each fragment has the same barcode as the source molecule.</p></div><figure class="product-photo"><img src="https://www.elementbiosciences.com/uploads/products/photos/_productPhoto/Step-2-Distribute-Prep.svg" width="20" height="7" alt="Step 2 Distribute Prep" loading="lazy" ></figure><div class="product-text"><h3><strong>Sequence</strong></h3><p>The AVITI System sequences the fragments as short reads. Subsequent demultiplexing groups the short reads by barcode.</p></div><figure class="product-photo"><img src="https://www.elementbiosciences.com/uploads/products/photos/_productPhoto/Step-3-Sequence.svg" width="20" height="7" alt="Step 3 Sequence" loading="lazy" ></figure><div class="product-text"><h3><strong>Reassemble</strong></h3><p><em>De novo </em>assembly reassembles overlapping short reads into the original full-length molecule.</p></div><figure class="product-photo"><img src="https://www.elementbiosciences.com/uploads/products/photos/_productPhoto/Step-4-Reassemble.svg" width="20" height="10" alt="Step 4 Reassemble" loading="lazy" ></figure></div></div></div></section><section class="product-section"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">LoopSeq Specifications</h2><div class="product-section__content"><div class="product-text"><p>LoopSeq for AVITI brings the proprietary, barcode-based long-read technology into the low-cost, high-quality avidite sequencing environment. The purchase of a LoopSeq product supplies library prep reagents, barcodes, and indexes packaged in multiple kits.</p></div><div class="product-table"><p class="product-table__tip">Swipe left in the table to show more columns.</p><table><thead><tr><th>Specification</th><th>16S LoopSeq for AVITI and 16S LoopSeq</th><th>Amplicon LoopSeq for AVITI and LoopSeq</th></tr></thead><tbody><tr><td width="122" valign="top"><p>Species</p></td><td width="254" valign="top"><p>Bacterial</p></td><td width="247" valign="top"><p>Any</p></td></tr><tr><td width="122" valign="top"><p>Specialized sample types</p></td><td width="254" valign="top"><p>Microbial</p></td><td width="247" valign="top"><p>Genomes, metagenomes, PCR products, synthetic DNA libraries, viruses</p></td></tr><tr><td width="122" valign="top"><p>Input requirement</p></td><td width="254" valign="top"><p>0.1–1 ng purified genomic DNA (gDNA) in 2 μl</p></td><td width="247" valign="top"><p>6 ng DNA in 30 µl</p></td></tr><tr><td width="122" valign="top"><p>Size range</p></td><td width="254" valign="top"><p>Not applicable</p></td><td width="247" valign="top"><p>0.5–6 kb</p></td></tr><tr><td width="122" valign="top"><p>Storage temperature</p></td><td width="254" valign="top"><p>-25°C to -15°C</p></td><td width="247" valign="top"><p>-25°C to -15°C</p></td></tr><tr><td width="122" valign="top"><p>Protocol time</p></td><td width="254" valign="top"><p>~16 hours, including ~5 hours of hands-on time*</p></td><td width="247" valign="top"><p>~16 hours, including ~5 hours of hands-on time*</p></td></tr><tr><td width="122" valign="top"><p>Read length</p></td><td width="254" valign="top"><p>2 x 151 bp</p></td><td width="247" valign="top"><p>2 x 151 bp</p></td></tr><tr><td width="122" valign="top"><p>System compatibility</p></td><td width="254" valign="top"><p>AVITI System and other short-read platforms</p></td><td width="247" valign="top"><p>AVITI System and other short-read platforms</p></td></tr></tbody></table><p class="product-footnote">* Exclusive of circularization</p></div></div></div></div></section><section class="product-section"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">Catalog Numbers</h2><div class="product-section__content"><div class="product-table"><p class="product-table__tip">Swipe left in the table to show more columns.</p><table><thead><tr><th>Product</th><th>Catalog #</th></tr></thead><tbody><tr><td width="443" nowrap="nowrap" valign="top"><p>16S LoopSeq for AVITI</p></td><td width="152" nowrap="nowrap" valign="top"><p>840-00001</p></td></tr><tr><td width="443" nowrap="nowrap" valign="top"><p>Amplicon LoopSeq for AVITI</p></td><td width="152" nowrap="nowrap" valign="top"><p>840-00002</p></td></tr><tr><td width="443" nowrap="nowrap" valign="top"><p>Extension LoopSeq for AVITI</p></td><td width="152" nowrap="nowrap" valign="top"><p>840-00003</p></td></tr><tr><td width="443" nowrap="nowrap" valign="top"><p>Element Elevate Library Circularization Kit</p></td><td width="152" nowrap="nowrap" valign="top"><p>830-00001</p></td></tr><tr><td width="443" nowrap="nowrap" valign="top"><p>16S LoopSeq</p></td><td width="152" nowrap="nowrap" valign="top"><p>840-10001</p></td></tr><tr><td width="443" nowrap="nowrap" valign="top"><p>Amplicon LoopSeq</p></td><td width="152" nowrap="nowrap" valign="top"><p>840-10002</p></td></tr><tr><td width="443" nowrap="nowrap" valign="top"><p>Extension LoopSeq</p></td><td width="152" nowrap="nowrap" valign="top"><p>840-10003</p></td></tr></tbody></table></div></div></div></div></section><section class="product-section"><div class="product-section__container"><div class="product-section__wrapper"><h2 class="product-section__heading">Get In Touch</h2><div class="product-section__content"><form id="contact_us" method="post" class="form product-form" data-ref="form" data-handle="contact" data-recaptcha="v2_invisible" data-recaptcha-key="6Lf9AJ8fAAAAAJV8KV3IFsNp8pko0qr56kyvjO3c" data-recaptcha-lazy-load data-freeform data-id="8a06e8-form-q81yPZdRB-XalPvNPkE-6U9sLbmNPDVo3X6P1HZXc9ddZH46OclfLkYnT6Q7" data-disable-submit data-show-spinner data-auto-scroll data-show-loading-text data-loading-text="Sending..." data-success-message="Thank you for your contact request!" data-error-message="Sorry, there was an error submitting the form. 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class="card-link__text">LoopSeq brochure</div><div class="card-link__button"><svg><use xlink:href="/assets/img/icons/glyphs.svg?v=#download"></use></svg></div></div></a><a href="/resources/16s-loopseq-aviti-user-guide-ma-00020" class="card-link -download" ><div class="card-link__icon"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 153.57 133.27"><defs><style> .ntofjkpguv-blue { fill: #233064; } .ntofjkpguv-green { fill: #07753c; } </style></defs><path class="ntofjkpguv-blue" 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class="card-link__text">16S LoopSeq for AVITI workflow guide</div><div class="card-link__button"><svg><use xlink:href="/assets/img/icons/glyphs.svg?v=#download"></use></svg></div></div></a><a href="/resources/amplicon-loopseq-aviti-user-guide-ma-00023" class="card-link -download" ><div class="card-link__icon"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 153.57 133.27"><defs><style> .vlldvogfmx-blue { fill: #233064; } .vlldvogfmx-green { fill: #07753c; } </style></defs><path class="vlldvogfmx-blue" 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