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href="https://www.reactionbiology.com/services/cell-based-assays/">Cell-based Assays</a></li> <li>3D ProLiFiler Spheroid Cancer Cell Panel Screening Service</li> </ol> </nav> </div> </div> <div class="view-container view-simple-page-conent"> <div class="container"> <article id="post-7070" class="post-7070 page type-page status-publish hentry" itemtype="https://schema.org/CreativeWork" itemscope=""> <div class="entry-content entry-content-servises "> <h1>3D ProLiFiler™ Spheroid Cancer Cell Panel Screening Service</h1> <div class="text-mit-bild-text"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <p>The three-dimensional (3D) ProLiFiler<sup>TM</sup> spheroid panel screening service enables scientists to analyze the therapeutic potency and tumor-penetrating efficacy of new anti-cancer compounds on 3D spheroids grown from a panel of 40 distinctive cell lines derived from 13 tumor entities. This service provides an efficient way to check for compound efficacy against 40 common tumor cell lines.</p> <p>3D tumor spheroids, also known as cancer spheroids, are composed of tumor cells in various proliferative and metabolic states and have demonstrated to mimic complex physiological tumor processes like tumorigenesis, microenvironment, heterogeneity, and tumor-immune interaction. Testing cancer drug candidates on a panel of 3D spheroids grown from distinct tumor cell lines with important clinical mutations yields high-quality data from a physiologically relevant <em>in vitro</em> assay format, making it a valuable tool for lead candidate selection for further <em>in vivo</em> studies.</p> <p>3D ProLiFiler<sup>TM</sup> spheroid cancer cell panel screening key advantages:</p> <ul> <li>Evaluate drug responses in using a 3D model across 40 cell lines to better understand compound effectiveness and prioritize the most suitable and potent compounds before moving to <em>in vivo</em> studies.</li> <li>A more physiologically relevant model that mimics <em>in vivo</em> tumor biology, signaling cascades, and tumor progression with reproduced gradients of oxygenation, nutrition, and pH.</li> <li>3D spheroids reflect the tumor microenvironment by modeling 3D cell-cell attachment, interaction, and cell-ECM interactions.</li> </ul> </div> <div class="contact-buttons"> <div 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images-1 contact-buttons- paragraph--type--text-with-image paragraph--view-mode--default"><div class="container center"><div class="text-wrapper"><h3>Distribution of 3D ProLiFiler™ Spheroid Panel Screen Entities</h3> <div class="text-mit-bild-text"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <p>The 3D ProLiFiler<sup>TM</sup> spheroid panel screening service has 40 cell lines derived from 13 distinct entities with clinically important oncogenic mutations available for your next cell panel screening project.</p> <p><a href="/contact/contact-us/">Please inquire</a> to obtain the list of included cell lines.</p> </div> </div> <div class="text-mit-bild-bild field field--name-field-images field--type-image field--label-hidden field__items"> <div class="text-mit-bild-bild-rahmen"> <div class="field__item"> <p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-7072" src="https://reactionbio.wpengine.com/wp-content/uploads/2023/10/3D-ProLiFiler-entities-graph.png" alt="" width="800" height="799" srcset="https://www.reactionbiology.com/wp-content/uploads/2023/10/3D-ProLiFiler-entities-graph.png 800w, https://www.reactionbiology.com/wp-content/uploads/2023/10/3D-ProLiFiler-entities-graph-300x300.png 300w, https://www.reactionbiology.com/wp-content/uploads/2023/10/3D-ProLiFiler-entities-graph-150x150.png 150w, https://www.reactionbiology.com/wp-content/uploads/2023/10/3D-ProLiFiler-entities-graph-768x767.png 768w" sizes="(max-width: 800px) 100vw, 800px" /></p> <div class="caption">3D ProLiFiler Entities</div> </div> </div> </div> </div></div></div><div id="dps3" class="paragraph paragraph--type--tab-container paragraph--view-mode--default"><div class="container center"><h2>3D Spheroid Panel Screen Details</h2> <div class="tab-container"><ul class="tab-navigation"><li class="tab-active" data-id="#tab-dps321">Assay Description</li><li data-id="#tab-dps322">Case Study</li></ul><div class="field field--name-field-paragraph field--type-entity-reference-revisions field--label-hidden field__items"><div id="tab-dps321" class="tab-content-active paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay Description</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><h3><span class="txt-blue">Assay Description</span></h3> <p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-7073" src="https://reactionbio.wpengine.com/wp-content/uploads/2023/10/230812_3D_ProLiFiler_Assay.png" alt="" width="2000" height="580" srcset="https://www.reactionbiology.com/wp-content/uploads/2023/10/230812_3D_ProLiFiler_Assay.png 2000w, https://www.reactionbiology.com/wp-content/uploads/2023/10/230812_3D_ProLiFiler_Assay-300x87.png 300w, https://www.reactionbiology.com/wp-content/uploads/2023/10/230812_3D_ProLiFiler_Assay-1024x297.png 1024w, https://www.reactionbiology.com/wp-content/uploads/2023/10/230812_3D_ProLiFiler_Assay-768x223.png 768w, https://www.reactionbiology.com/wp-content/uploads/2023/10/230812_3D_ProLiFiler_Assay-1536x445.png 1536w, https://www.reactionbiology.com/wp-content/uploads/2023/10/230812_3D_ProLiFiler_Assay-1080x313.png 1080w" sizes="(max-width: 2000px) 100vw, 2000px" /> <img loading="lazy" decoding="async" class="aligncenter size-full wp-image-7074" src="https://reactionbio.wpengine.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler.png" alt="" width="1588" height="342" srcset="https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler.png 1588w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-300x65.png 300w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-1024x221.png 1024w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-768x165.png 768w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-1536x331.png 1536w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-1080x233.png 1080w" sizes="(max-width: 1588px) 100vw, 1588px" /></p> <p>Tumor cells are incubated for 2 days in ultra-low attachment surface plates (384 well format) , shortly spun down, and allowed to autonomously self-assemble to form spheroids. The test compound is added and incubate for 6 days. Finally, cell plates are equilibrated to room temperature, and the viability of cells is determined with the CellTiter-Glo luminescent reagent based on the amount of ATP present.</p> </div></div></div></div><div id="tab-dps322" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Case Study</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><h4><span class="txt-blue"><strong>Case study: Characterization of MEK-inhibitor in 3D and 2D cell culture format</strong></span></h4> <p><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-7074" src="https://reactionbio.wpengine.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler.png" alt="" width="1588" height="342" srcset="https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler.png 1588w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-300x65.png 300w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-1024x221.png 1024w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-768x165.png 768w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-1536x331.png 1536w, https://www.reactionbiology.com/wp-content/uploads/2023/10/Case-Study-3D-ProLiFiler-1080x233.png 1080w" sizes="(max-width: 1588px) 100vw, 1588px" /></p> <p>Characterization of MEK-inhibitors in 3D cell panel format: Higher potency was demonstrated by a reference clinical MEK inhibitor in a 3D screening assay vs. a 2D format, ultimately targeting the MAPK kinase signaling cascade. MEK-Inhibitors show increased activity on cells, confirming different physiological situations of cells in 3D, while control proteasome inhibitor activity shows no effect. Black: 2D data, Red = 3D data.</p> </div></div></div></div></div></div></div></div><div id="dps4" class="paragraph paragraph--type--form paragraph--view-mode--default"><div class="container center"><div class="text-wrapper"><p><strong>Join the next 3D ProLiFiler<sup>TM </sup> <a href="https://www.reactionbiology.com/services/cell-based-assays/prolifiler-cell-line-panel-screening/prolifiler-schedule">screening schedule.</a> If you are interested in taking part please <a href="/contact/contact-us/">contact us</a>.</strong></p> </div></div></div><div id="dps5" class="paragraph paragraph--type--related-resources-container paragraph--view-mode--default"><div class="container center"><h2>Related Resources</h2><div class="field--name-field-paragraph related-resources-reference-wrapper"><div class="paragraph--type--related-resource node--type-related-resource"><div class="related-resource-wrapper"><div class="field--name-field-image"><div class="field field--name-field-image field--type-image 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