Search results for: P. S. Gôlo

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Gôlo</title> <meta name="description" content="Search results for: P. S. Gôlo"> <meta name="keywords" content="P. S. Gôlo"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="P. 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S. Gôlo"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: P. S. Gôlo</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> In vitro Control of Aedes aegypti Larvae Using Beauveria bassiana</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=R.%20O.%20B.%20Bitencourt">R. O. B. Bitencourt</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20S.%20Farias"> F. S. Farias</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20C.%20Freitas"> M. C. Freitas</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20J.%20R.%20Balduino"> C. J. R. Balduino</a>, <a href="https://publications.waset.org/abstracts/search?q=E.S.%20Mesquita"> E.S. Mesquita</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20R.%20C.%20Corval"> A. R. C. Corval</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20S.%20G%C3%B4lo"> P. S. Gôlo</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20G.%20Pontes"> E. G. Pontes</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20R.%20E.%20P.%20Bittencourt"> V. R. E. P. Bittencourt</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20C.%20Angelo"> I. C. Angelo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <em>Aedes aegypti </em>larval survival rate was assessed after exposure to blastopores or conidia (mineral oil-in-water formulation or aqueous suspension) of <em>Beauveria bassiana </em>CG 479 propagules (blastospores or conidia). Here, mineral oil was used in the fungal formulation to control <em>Aedes aegypti</em> larvae. 1%, 0.5% or 0.1% mineral oil-in-water solutions were used to evaluate mineral oil toxicity for mosquito larvae. In the oil toxicity test, 0.1% mineral oil solution reduced only 4.5% larval survival; accordingly, this concentration was chosen for fungal oil-in-water formulations. Aqueous suspensions were prepared using 0.01% Tween 80<sup>®</sup> in sterile dechlorinated water. <em>A. aegypti</em> larvae (L<sub>2</sub>) were exposed in aqueous suspensions or mineral oil-in-water fungal formulations at 1×107 propagules mL<sup>-1</sup>; the survival rate (assessed daily, for 7 days) and the median survival time (S<sub>50</sub>) were calculated. Seven days after the treatment, mosquito larvae survival rates were 8.56%, 16.22%, 58%, and 42.56% after exposure to oil-in-water blastospores, oil-in-water conidia, blastospores aqueous suspension and conidia aqueous suspension (respectively). Larvae exposed to 0.01% Tween 80<sup>®</sup> had 100% survival rate and the ones treated with 0.1% mineral oil-in-water had 95.11% survival rate. Larvae treated with conidia (regardless the presence of oil) or treated with blastospores formulation had survival median time (S<sub>50</sub>) ranging from one to two days. S<sub>50</sub> was not determined (ND) when larvae were exposed to blastospores aqueous suspension, 0.01% Tween 80<sup>®</sup> (aqueous control) or 0.1% mineral oil-in-water formulation (oil control). <em>B. bassiana</em> conidia and blastospores (mineral oil-in-water formulated or suspended in water) had potential to control <em>A. aegypti</em> mosquito larvae, despite mineral oil-in-water formulation yielded better results in comparison to aqueous suspensions. Here, <em>B. bassiana</em> CG 479 isolate is suggested as a potential biocontrol agent of <em>A. aegypti</em> mosquito larvae. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=blastospores" title="blastospores">blastospores</a>, <a href="https://publications.waset.org/abstracts/search?q=formulation" title=" formulation"> formulation</a>, <a href="https://publications.waset.org/abstracts/search?q=mosquitoes" title=" mosquitoes"> mosquitoes</a>, <a href="https://publications.waset.org/abstracts/search?q=conidia" title=" conidia"> conidia</a> </p> <a href="https://publications.waset.org/abstracts/87360/in-vitro-control-of-aedes-aegypti-larvae-using-beauveria-bassiana" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/87360.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">188</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Effect of Metarhizium robertsii in Rhipicephalus microplus hemocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jessica%20P.%20Fiorotti">Jessica P. Fiorotti</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20C.%20Freitas"> Maria C. Freitas</a>, <a href="https://publications.waset.org/abstracts/search?q=Caio%20%20J.%20B.%20Coutinho-Rodrigues"> Caio J. B. Coutinho-Rodrigues</a>, <a href="https://publications.waset.org/abstracts/search?q=Mariana%20G.%20Camargo"> Mariana G. Camargo</a>, <a href="https://publications.waset.org/abstracts/search?q=Emily%20S.%20Mesquita"> Emily S. Mesquita</a>, <a href="https://publications.waset.org/abstracts/search?q=Amanda%20R.%20C.%20Corval"> Amanda R. C. Corval</a>, <a href="https://publications.waset.org/abstracts/search?q=Ricardo%20O.%20B.%20Bitencourt"> Ricardo O. B. Bitencourt</a>, <a href="https://publications.waset.org/abstracts/search?q=Allan%20F.%20Marciano"> Allan F. Marciano</a>, <a href="https://publications.waset.org/abstracts/search?q=Diva%20D.%20Spadacci-Morena"> Diva D. Spadacci-Morena</a>, <a href="https://publications.waset.org/abstracts/search?q=Patricia%20S.%20Golo"> Patricia S. Golo</a>, <a href="https://publications.waset.org/abstracts/search?q=Isabele%20C.%20Angelo"> Isabele C. Angelo</a>, <a href="https://publications.waset.org/abstracts/search?q=Vania%20R.%20E.%20P.%20Bittencourt"> Vania R. E. P. Bittencourt</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The bovine tick, Rhipicephalus microplus, is an arthropod of great importance in veterinary medicine leading to anemia, weight loss, animals' leather depreciation and also acting as a vector of many pathogens. In this way, the parasitism causes a loss of 3.24 billion dollars per year in Brazil. Knowingly, entomopathogenic fungi act as natural controller of some arthropods, acting mainly by active penetration through the cuticle. However, it can also act on the hemolymph and through the production of mycotoxins. Hemocytes are responsible for the cellular immune response and participate in the processes of phagocytosis, nodulation and encapsulation and may undergo changes when challenged by pathogens. The aim of the present study was to evaluate changes in R. microplus hemocytes after inoculation of Metarhizium robertsii using transmission electron microscopy. The isolate ARSEF 2575 and 200 engorged R. microplus females were used. The groups were divided into control, in which the females were inoculated with 5 μL of sterile distilled water solution and 0.1% Tween 80, and a group inoculated with 5 μL of fungal suspension at the concentration of 10⁷ conidia mL⁻¹. The experiment was performed in duplicate and each group contained 50 females. Twenty-four hours after fungal inoculation, hemolymph was collected through the cuticle dorsal surface perforation of the tick females. After collection, the hemolymph samples were centrifuged at 500 x g for 3 minutes at 4 °C, the plasma was discarded and the hemocyte pellet was resuspended in 50 μl PBS. The suspension material was fixed in 2% glutaraldehyde in Millonig buffer for three hours. After fixation, the material was centrifuged at 500 x g for 3 minutes, the supernatant was discarded and the cells were resuspended in a wash solution. Subsequently, the cells were post-fixed with 1% osmium tetroxide in phosphate buffer for one hour at room temperature and dehydrated in increasing concentrations of ethanol, and then embedded in Epon resin. The ultrathin sections were examined under the LEO EM 906E transmission electron microscopy at 80kV. The ultrastructural results revealed that.in control group, the cells were considered intact, in which the granulocytes were observed with granules of different electrodensities, intact mitochondria and cytoplasm without vacuolization. In addition, granulocytes showed plasma membrane projections similar to pseudopodia. Plasmatocytes presented as irregularly shaped cells, with the eccentric nucleus, agranular cytoplasm and some cells presented pseudopodia. Nevertheless, in the group exposed to the fungus, most of the cells presented in degeneration. The granulocytes found had fewer granules in the cytoplasm and more vacuoles. Plasmatocytes, after treatment, presented many vacuoles also in the cytoplasm and the lysosomes presented great amount of electrodense material in their interior. Thus, the results suggest that the fungus has a depressant action in the immune system of the tick, not only by the cell degranulation, but also suggesting that this leads to morphological changes in the hemocytes and may even trigger processes such as phagocytosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bovine%20tick" title="bovine tick">bovine tick</a>, <a href="https://publications.waset.org/abstracts/search?q=cellular%20defense" title=" cellular defense"> cellular defense</a>, <a href="https://publications.waset.org/abstracts/search?q=entomopathogenic%20fungi" title=" entomopathogenic fungi"> entomopathogenic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=immune%20response" title=" immune response"> immune response</a> </p> <a href="https://publications.waset.org/abstracts/89287/effect-of-metarhizium-robertsii-in-rhipicephalus-microplus-hemocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/89287.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">189</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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Search results for: P. S. Gôlo