Simple Plex Human TIMP-1 Assay Cartridge (SPCKB-PS-001442)

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data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Simple Plex Human TIMP-1 Cartridge </h1> <h2 class="font-weight-normal h4"> ProteinSimple, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">SPCKB-PS-001442</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div class="col-auto d-flex align-self-center"> <div> <a href="#product-documents" class="datasheet_link product-datasheets" rel="nofollow">Product Datasheet / COA</a> </div> </div> </div> </div> <div id="new_version_available"> </div> <div class="row mb-2" id="extended-description"> <div class="col-md-10 extended-description-wrapper" id="extended-description-wrapper"> Simple Plex Human TIMP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. </div> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-proteinsimple.svg" alt="ProteinSimple, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> <div id="product_alerts_wrapper"><article class="product-data-wrapper"> </article> </div> </div> <div class="row border-top mb-2" id="images_atc_orderinfo_wrapper"><div class="col-xl-9 mt-2" id="image_atc_keydetails_wrapper"><div class="row" id="image_atc_wrapper"><div class="col-xl-4 order-xl-first" id="image_wrapper"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="proteinsimple"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.rndsystems.com/categoryimages/proteinsimple_simple-plex-single-analyte-assay-cartridges.png" alt="Human TIMP-1 Ella Assay" title="Human TIMP-1 Ella Assay" data-caption="" data-badges="" data-application="N/A" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/spckb-ps-001442_simple-plex-cartridge-kit-for-72-samples-1-analyte-for-use-with-human-plasma-serum-1952020172648.png" data-alt="Human TIMP-1 Ella Assay Standard Curve" title="Human TIMP-1 Ella Assay Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/spckb-ps-001442_simple-plex-cartridge-kit-for-72-samples-1-analyte-for-use-with-human-plasma-serum-1952020172821.jpg" data-alt="Human TIMP-1 Ella Assay Linearity" title="Human TIMP-1 Ella Assay Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </article> </div> <div class="col-xl-8" id="atc_wrapper"><article class="product-data-wrapper position-relative align-vertical"> <div id="order_detail_wrapper" class="order_detail_wrapper"> <h3>Customize Your Simple Plex Cartridge</h3> <p>Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.</p> <hr class="a___border-dashed my-4"> <div class="text-right mb-2"> <a class="btn btn-primary text-white" href="/resources/simple-plex-panel-builder"> Build My Cartridge </a> </div> </div> </article> </div> </div> <div class="row datapoint_list border-top" id="key_details_wrapper"><div class="col-xl-12 mt-2" id="key_details_col_wrapper"></div> </div> </div> <div class="col-xl-3 mt-2" id="orderinfo_guarantee_wrapper"><div id="order_information_wrapper"> <div id="product_shipping_block_wrapper" data-brand="proteinsimple" data-shipping="" data-source="ep-data" data-sub_category_id="136"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"><span class="sr-only">Loading...</span></div> </div> </div> <div id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> <div class="datapoint_list" id="key_details"><h3>Key Product Details</h3><table class="tablesaw tablesaw-stack table" id="key_details_table_wrapper"><tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Assay Length: </td> <td class="product-data-value py-2 align-top w-75 text-break"> 90 minutes </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Sample Type & Volume Required Per Well: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL), Cerebrospinal Fluid (10 uL) </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Sensitivity: </td> <td class="product-data-value py-2 align-top w-75 text-break"> 0.34 pg/mL </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Assay Range: </td> <td class="product-data-value py-2 align-top w-75 text-break"> 1.05 - 10,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid) </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Simple Plex Human TIMP-1 Cartridge Summary: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Simple Plex assay for the detection of human Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) in cell <span>culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).</span> </td> </tr> </table> </div> <div class="mt-2" id="prod_key_details_footnote"><p>*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix.  Serial dilution may be necessary to achieve some of the final sample volumes represented.</p> </div> <div id="view_technical_detail_links"><div class="text-center"> <a id="view_technical" href="#about-data-tab-tab">View Full Technical Details for Simple Plex Human TIMP-1 Cartridge</a> </div> </div> </div> <div class="tab-content rounded-bottom shadow border-0 mb-3"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="technical-data-tab-tab" href="#technical-data-tab" data-toggle="tab" role="tab" aria-controls="region-5791588863627" aria-selected="true" >Technical Data</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-tab-tab" href="#citations-tab" data-toggle="tab" role="tab" aria-controls="region-8571587488941" aria-selected="false" >Citations</a> </li> </ul> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4" id="technical-data-tab" data-tab-label="Technical Data" role="tabpanel" aria-labelledby="technical-data-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="product-specifications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#product-specifications" id="product-specifications_li" >Product Specifications</a> </li> <li id="precision_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#precision" id="precision_li" >Precision</a> </li> <li id="recovery_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#recovery" id="recovery_li" >Recovery</a> </li> <li id="linearity_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#linearity" id="linearity_li" >Linearity</a> </li> <li id="scientific-data_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#scientific-data" id="scientific-data_li" >Scientific Data</a> </li> <li id="preparation-and-storage_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#preparation-and-storage" id="preparation-and-storage_li" >Preparation and Storage</a> </li> <li id="background_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#background" id="background_li" >Background</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="product-specifications" class="nav-link px-0" data-anchor-label="Product Specifications"><div id="product_specifications_section"><h3>Product Specifications for Simple Plex Human TIMP-1 Cartridge</h3><table class="table-bordered table" id="table_wrapper"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Assay Type</td> <td class="product-data-value p-3 px-4 text-break"> Automated sandwich ELISA </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Format</td> <td class="product-data-value p-3 px-4 text-break"> Microfluidic cartridge containing three Glass Nano Reactors (GNR) </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Reactivity</td> <td class="product-data-value p-3 px-4 text-break"> Human </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Specificity</td> <td class="product-data-value p-3 px-4 text-break"> Natural and recombinant human TIMP-1. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Cross-reactivity</td> <td class="product-data-value p-3 px-4 text-break"> < 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Interference</td> <td class="product-data-value p-3 px-4 text-break"> Interference observed with 1 or more available related molecules. </td> </tr> </table> </div> </div> <div id="precision" class="nav-link px-0" data-anchor-label="Precision"><div id="precision_wrapper"><h3>Precision </h3> <p><div><strong>Intra-Assay Precision (Precision within an assay)</strong> Each control was tested 16 times in one assay</div><div><strong>Inter-Assay Precision (Precision between assays)</strong> Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents</div></p> <p class="precision_title"><strong>Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid</strong></p> <table class="ds_table_html precision_table table-bordered"> <thead> <tr> <th class="p1 p-3 w-25"></th> <th colspan="2" class="w-25 p-3">Intra-Assay Precision</th> <th colspan="2" class="p-3 w-25">Inter-Assay Precision</th> </tr> </thead> <tbody> <tr> <td class="p-3"><strong>Sample</strong></td> <td class="p-3">1</td> <td class="p-3">2</td> <td class="p-3">1</td> <td class="p-3">2</td> </tr> <tr> <td class="p-3"><strong>n</strong></td> <td class="p-3">16</td> <td class="p-3">16</td> <td class="p-3">32</td> <td class="p-3">33</td> </tr> <tr> <td class="p-3"><strong>Mean (pg/mL)</strong></td> <td class="p-3">57.8</td> <td class="p-3">2915</td> <td class="p-3">54.5</td> <td class="p-3">2833</td> </tr> <tr> <td class="p-3"><strong>Standard Deviation</strong></td> <td class="p-3">4.86</td> <td class="p-3">152</td> <td class="p-3">5.97</td> <td class="p-3">259</td> </tr> <tr> <td class="p-3"><strong>CV%</strong></td> <td class="p-3">8.4</td> <td class="p-3">5.2</td> <td class="p-3">10.9</td> <td class="p-3">9.1</td> </tr> </tbody> </table> </div> </div> <div id="recovery" class="nav-link px-0" data-anchor-label="Recovery"><div id="elisa_recovery"><h3>Recovery</h3> <div> <p>Recovery at three different spiked concentrations within the range of the assay was evaluated.<p> </div> <table class="ds_table_html recovery_table table-bordered"> <thead> <th class="p-3">Sample Type</th> <th class="p-3">Average % Recovery</th> <th class="p-3">Range %</th> </thead> <tr> <td class="p-3">Cell Culture Media (n=2)</td> <td class="p-3">112</td> <td class="p-3">112-113</td> </tr> </table> </div> </div> <div id="linearity" class="nav-link px-0" data-anchor-label="Linearity"><div id="linerarity_wrapper"><h3>Linearity</h3><article class="product-data-wrapper"> Samples containing and/or spiked with high concentrations of human TIMP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay </article> <div class="image-basic"> <img src="https://resources.bio-techne.com/images/products/spckb-ps-001442_simple-plex-cartridge-kit-for-72-samples-1-analyte-for-use-with-human-plasma-serum-1952020172821.jpg" alt="Human TIMP-1 Ella Assay Linearity" title="Human TIMP-1 Ella Assay Linearity" /> </div> </div> </div> <div id="scientific-data" class="nav-link px-0" data-anchor-label="Scientific Data"><table class="product-data-wrapper tablesaw tablesaw-stack table" id="data_image_table_wrapper"><h3>Scientific Data Examples for Simple Plex Human TIMP-1 Cartridge</h3><article class="product-data-wrapper"> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/spckb-ps-001442_simple-plex-cartridge-kit-for-72-samples-1-analyte-for-use-with-human-plasma-serum-1952020172648.png" data-srcset="https://resources.bio-techne.com/images/products/spckb-ps-001442_simple-plex-cartridge-kit-for-72-samples-1-analyte-for-use-with-human-plasma-serum-1952020172648.png" alt="Human TIMP-1 Ella Assay Standard Curve" title="Human TIMP-1 Ella Assay Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Human TIMP-1 Ella Assay Standard Curve</strong> </p> </td> </tr> </article> </table> </div> <div id="preparation-and-storage" class="nav-link px-0" data-anchor-label="Preparation and Storage"><div id="elisa_preparation_storage"><h3>Preparation and Storage</h3><table class="table-bordered table" id="elisa_preparation_storage_table"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Shipping</td> <td class="product-data-value p-3 px-4 text-break"> The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Stability & Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store the unopened product at 2 - 8 °C. Do not use past expiration date. </td> </tr> </table> </div> </div> <div id="background" class="nav-link px-0" data-anchor-label="Background"><div id="background_information_wrapper"> <div id="background_information_wrapper"> <h3> Background: TIMP-1 </h3> <article class="product-data-wrapper"> TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14. </article> <table class="tablesaw tablesaw-stack table-bordered" id="prep_storage_wrapper"> <thead style="display:none;"> <th>Item</th> <th>Value</th> </thead> <tbody> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right">Long Name</td> <td class="product-data-value w-75 p-3 px-4"> Tissue Inhibitors of Metalloproteinases 1 </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right">Entrez Gene IDs</td> <td class="product-data-value w-75 p-3 px-4"> 7076 (Human); 21857 (Mouse); 116510 (Rat) </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right">Alternate Names</td> <td class="product-data-value w-75 p-3 px-4"> CLGI, Collagenase inhibitor, EPATIMP-1, EPO, Erythroid-potentiating activity, FLJ90373, Fibroblast collagenase inhibitor, HCI, TIMP metallopeptidase inhibitor 1, TIMP-1, TIMP1, TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, Tissue inhibitor of metalloproteinases 1, collagenase inhibitor), erythroid potentiating activity, metalloproteinase inhibitor 1 </td> </tr> </tbody> </table> </div> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4" id="citations-tab" data-tab-label="Citations" role="tabpanel" aria-labelledby="citations-tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> </div> </div> <div id="citations"> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="SPCKB-PS-001442"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> </div> </div> </div> </div> <div id="compatible-instruments-wrapper"><div id="compatible-intruments-anchor"></div> <div id="compatible-instruments" class="top_region px-0"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">Compatible Instruments</h3> </div> <div id="carouselCompatiableInstrument" class="carousel slide" data-ride="carousel"> <div class="carousel-inner"> <div class="carousel-item active"> <div class="row w-75 m-auto"> <div class="col-md-4"> <img src="https://resources.rndsystems.com/images/datasheets/Ella_System_600100_ELISA_2642.png" alt="Ella Automated Immunoassay System" title="Ella Automated Immunoassay System" loading="lazy"/> </div> <div class="col-md-8"> <div class="carousel-caption position-initial"> <h4>Ella - Automated Immunoassay Platform | ProteinSimple </h4> <article>Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible. </article> <div class="mt-3"> <a href="/p/simple-plex/ella-automated-immunoassay-system_600-100" class="btn btn-primary"> Learn More </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div id="product-documents"> <div id="datasheet-accordion" class="accordion border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">Product Documents for Simple Plex Human TIMP-1 Cartridge</h3> </div> <div class="vtab-section" data-section="Product Datasheets"> <div class="card-header rounded-top"> <a class="" data-toggle="collapse" data-target="#collapse_two" aria-expanded="true" aria-controls="collapse_two" id="product-datasheets-link"> <h5 class="mb-0"> Product Datasheets </h5> </a> </div> <div id="collapse_two" class="collapse show prod_datasheet_link" aria-labelledby="heading_two" data-parent="#datasheet-accordion"> <div class="card-body"> <div class="row"> 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Simple Plex Human TIMP-1 Assay Cartridge (SPCKB-PS-001442)