CINXE.COM

<!DOCTYPE html> <html lang="en" class="grade-c"> <head> <title>Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor | Nature Communications</title> <link rel="alternate" type="application/rss+xml" href="https://www.nature.com/ncomms.rss"/> <script id="save-data-connection-testing"> function hasConnection() { return navigator.connection || navigator.mozConnection || navigator.webkitConnection || navigator.msConnection; } function createLink(src) { var preloadLink = document.createElement("link"); preloadLink.rel = "preload"; preloadLink.href = src; preloadLink.as = "font"; preloadLink.type = "font/woff2"; preloadLink.crossOrigin = ""; document.head.insertBefore(preloadLink, document.head.firstChild); } var connectionDetail = { saveDataEnabled: false, slowConnection: false }; var connection = hasConnection(); if (connection) { connectionDetail.saveDataEnabled = connection.saveData; if (/\slow-2g|2g/.test(connection.effectiveType)) { connectionDetail.slowConnection = true; } } if (!(connectionDetail.saveDataEnabled || connectionDetail.slowConnection)) { createLink("/static/fonts/HardingText-Regular-Web-cecd90984f.woff2"); } else { document.documentElement.classList.add('save-data'); } </script> <link rel="preconnect" href="https://cmp.nature.com" crossorigin> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="applicable-device" content="pc,mobile"> <meta name="viewport" content="width=device-width,initial-scale=1.0,maximum-scale=5,user-scalable=yes"> <meta name="360-site-verification" content="5a2dc4ab3fcb9b0393241ffbbb490480" /> <script data-test="dataLayer"> window.dataLayer = [{"content":{"category":{"contentType":"article","legacy":{"webtrendsPrimaryArticleType":"research","webtrendsSubjectTerms":"peptides;protein-design;receptor-pharmacology","webtrendsContentCategory":null,"webtrendsContentCollection":null,"webtrendsContentGroup":"Nature Communications","webtrendsContentGroupType":null,"webtrendsContentSubGroup":"Article","status":null}},"article":{"doi":"10.1038/s41467-023-43718-w"},"attributes":{"cms":null,"deliveryPlatform":"oscar","copyright":{"open":true,"legacy":{"webtrendsLicenceType":"http://creativecommons.org/licenses/by/4.0/"}}},"contentInfo":{"authors":["Edin Muratspahić","Kristine Deibler","Jianming Han","Nataša Tomašević","Kirtikumar B. Jadhav","Aina-Leonor Olivé-Marti","Nadine Hochrainer","Roland Hellinger","Johannes Koehbach","Jonathan F. Fay","Mohammad Homaidur Rahman","Lamees Hegazy","Timothy W. Craven","Balazs R. Varga","Gaurav Bhardwaj","Kevin Appourchaux","Susruta Majumdar","Markus Muttenthaler","Parisa Hosseinzadeh","David J. Craik","Mariana Spetea","Tao Che","David Baker","Christian W. Gruber"],"publishedAt":1701820800,"publishedAtString":"2023-12-06","title":"Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor","legacy":null,"publishedAtTime":null,"documentType":"aplusplus","subjects":"Peptides,Protein design,Receptor pharmacology"},"journal":{"pcode":"ncomms","title":"nature communications","volume":"14","issue":"1","id":41467,"publishingModel":"Open Access"},"authorization":{"status":true},"features":[{"name":"furtherReadingSection","present":true}],"collection":null},"page":{"category":{"pageType":"article"},"attributes":{"template":"mosaic","featureFlags":[{"name":"nature-onwards-journey","active":false}],"testGroup":null},"search":null},"privacy":{},"version":"1.0.0","product":null,"session":null,"user":null,"backHalfContent":true,"country":"HK","hasBody":true,"uneditedManuscript":false,"twitterId":["o3xnx","o43y9","o3ef7"],"baiduId":"d38bce82bcb44717ccc29a90c4b781ea","japan":false}]; window.dataLayer.push({ ga4MeasurementId: 'G-ERRNTNZ807', ga360TrackingId: 'UA-71668177-1', twitterId: ['3xnx', 'o43y9', 'o3ef7'], baiduId: 'd38bce82bcb44717ccc29a90c4b781ea', ga4ServerUrl: 'https://collect.nature.com', imprint: 'nature' }); </script> <script> (function(w, d) { w.config = w.config || {}; w.config.mustardcut = false; if (w.matchMedia && w.matchMedia('only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark)').matches) { w.config.mustardcut = true; d.classList.add('js'); d.classList.remove('grade-c'); d.classList.remove('no-js'); } })(window, document.documentElement); </script> <style>@media only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark) { .c-article-editorial-summary__container .c-article-editorial-summary__article-title,.c-card--major .c-card__title,.c-card__title,.u-h2,.u-h3,h2,h3{-webkit-font-smoothing:antialiased;font-family:Harding,Palatino,serif;font-weight:700;letter-spacing:-.0117156rem}.c-article-editorial-summary__container .c-article-editorial-summary__article-title,.c-card__title,.u-h3,h3{font-size:1.25rem;line-height:1.4rem}.c-reading-companion__figure-title,.u-h4,h4{-webkit-font-smoothing:antialiased;font-weight:700;line-height:1.4rem}html{text-size-adjust:100%;box-sizing:border-box;font-size:100%;height:100%;line-height:1.15;overflow-y:scroll}body{background:#eee;color:#222;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:1.125rem;line-height:1.76;margin:0;min-height:100%}details,main{display:block}h1{font-size:2em;margin:.67em 0}a,sup{vertical-align:baseline}a{background-color:transparent;color:#069;overflow-wrap:break-word;text-decoration:underline;text-decoration-skip-ink:auto;word-break:break-word}b{font-weight:bolder}sup{font-size:75%;line-height:0;position:relative;top:-.5em}img{border:0;height:auto;max-width:100%;vertical-align:middle}button,input,select{font-family:inherit;font-size:100%;line-height:1.15;margin:0}button,input{overflow:visible}button,select{text-transform:none}[type=submit],button{-webkit-appearance:button}[type=checkbox]{box-sizing:border-box;padding:0}summary{display:list-item}[hidden]{display:none}button{border-radius:0;cursor:pointer;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif}h1{-webkit-font-smoothing:antialiased;font-family:Harding,Palatino,serif;font-size:2rem;font-weight:700;letter-spacing:-.0390625rem;line-height:2.25rem}.c-card--major .c-card__title,.u-h2,.u-h3,h2{font-family:Harding,Palatino,serif;letter-spacing:-.0117156rem}.c-card--major .c-card__title,.u-h2,h2{-webkit-font-smoothing:antialiased;font-size:1.5rem;font-weight:700;line-height:1.6rem}.u-h3{font-size:1.25rem}.c-card__title,.c-reading-companion__figure-title,.u-h3,.u-h4,h4,h5,h6{-webkit-font-smoothing:antialiased;font-weight:700;line-height:1.4rem}.c-article-editorial-summary__container .c-article-editorial-summary__article-title,.c-card__title,h3{font-family:Harding,Palatino,serif;font-size:1.25rem}.c-article-editorial-summary__container .c-article-editorial-summary__article-title,h3{-webkit-font-smoothing:antialiased;font-weight:700;letter-spacing:-.0117156rem;line-height:1.4rem}.c-reading-companion__figure-title,.u-h4,h4{font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:1.125rem;letter-spacing:-.0117156rem}button:focus{outline:3px solid #fece3e;will-change:transform}input+label{padding-left:.5em}nav ol,nav ul{list-style:none none}p:empty{display:none}.sans-serif{font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif}.article-page{background:#fff}.c-article-header{font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;margin-bottom:40px}.c-article-identifiers{color:#6f6f6f;display:flex;flex-wrap:wrap;font-size:1rem;line-height:1.3;list-style:none;margin:0 0 8px;padding:0}.c-article-identifiers__item{border-right:1px solid #6f6f6f;list-style:none;margin-right:8px;padding-right:8px}.c-article-identifiers__item:last-child{border-right:0;margin-right:0;padding-right:0}.c-article-title{font-size:1.5rem;line-height:1.25;margin:0 0 16px}@media only screen and (min-width:768px){.c-article-title{font-size:1.875rem;line-height:1.2}}.c-article-author-list{display:inline;font-size:1rem;list-style:none;margin:0 8px 0 0;padding:0;width:100%}.c-article-author-list__item{display:inline;padding-right:0}.c-article-author-list svg{margin-left:4px}.c-article-author-list__show-more{display:none;margin-right:4px}.c-article-author-list__button,.js .c-article-author-list__item--hide,.js .c-article-author-list__show-more{display:none}.js .c-article-author-list--long .c-article-author-list__show-more,.js .c-article-author-list--long+.c-article-author-list__button{display:inline}@media only screen and (max-width:539px){.js .c-article-author-list__item--hide-small-screen{display:none}.js .c-article-author-list--short .c-article-author-list__show-more,.js .c-article-author-list--short+.c-article-author-list__button{display:inline}}#uptodate-client,.js .c-article-author-list--expanded .c-article-author-list__show-more{display:none!important}.js .c-article-author-list--expanded .c-article-author-list__item--hide-small-screen{display:inline!important}.c-article-author-list__button,.c-button-author-list{background:#ebf1f5;border:4px solid #ebf1f5;border-radius:20px;color:#666;font-size:.875rem;line-height:1.4;padding:2px 11px 2px 8px;text-decoration:none}.c-article-author-list__button svg,.c-button-author-list svg{margin:1px 4px 0 0}.c-article-author-list__button:hover,.c-button-author-list:hover{background:#069;border-color:transparent;color:#fff}.c-article-info-details{font-size:1rem;margin-bottom:8px;margin-top:16px}.c-article-info-details__cite-as{border-left:1px solid #6f6f6f;margin-left:8px;padding-left:8px}.c-article-metrics-bar{display:flex;flex-wrap:wrap;font-size:1rem;line-height:1.3}.c-article-metrics-bar__wrapper{margin:16px 0}.c-article-metrics-bar__item{align-items:baseline;border-right:1px solid #6f6f6f;margin-right:8px}.c-article-metrics-bar__item:last-child{border-right:0}.c-article-metrics-bar__count{font-weight:700;margin:0}.c-article-metrics-bar__label{color:#626262;font-style:normal;font-weight:400;margin:0 10px 0 5px}.c-article-metrics-bar__details{margin:0}.c-article-main-column{font-family:Harding,Palatino,serif;margin-right:8.6%;width:60.2%}@media only screen and (max-width:1023px){.c-article-main-column{margin-right:0;width:100%}}.c-article-extras{float:left;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;width:31.2%}@media only screen and (max-width:1023px){.c-article-extras{display:none}}.c-article-associated-content__container .c-article-associated-content__title,.c-article-section__title{border-bottom:2px solid #d5d5d5;font-size:1.25rem;margin:0;padding-bottom:8px}@media only screen and (min-width:768px){.c-article-associated-content__container .c-article-associated-content__title,.c-article-section__title{font-size:1.5rem;line-height:1.24}}.c-article-associated-content__container .c-article-associated-content__title{margin-bottom:8px}.c-article-body p{margin-bottom:24px;margin-top:0}.c-article-section{clear:both}.c-article-section__content{margin-bottom:40px;padding-top:8px}@media only screen and (max-width:1023px){.c-article-section__content{padding-left:0}}.c-article-authors-search{margin-bottom:24px;margin-top:0}.c-article-authors-search__item,.c-article-authors-search__title{font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif}.c-article-authors-search__title{color:#626262;font-size:1.05rem;font-weight:700;margin:0;padding:0}.c-article-authors-search__item{font-size:1rem}.c-article-authors-search__text{margin:0}.c-article-license__badge,c-card__section{margin-top:8px}.c-code-block{border:1px solid #eee;font-family:monospace;margin:0 0 24px;padding:20px}.c-code-block__heading{font-weight:400;margin-bottom:16px}.c-code-block__line{display:block;overflow-wrap:break-word;white-space:pre-wrap}.c-article-share-box__no-sharelink-info{font-size:.813rem;font-weight:700;margin-bottom:24px;padding-top:4px}.c-article-share-box__only-read-input{border:1px solid #d5d5d5;box-sizing:content-box;display:inline-block;font-size:.875rem;font-weight:700;height:24px;margin-bottom:8px;padding:8px 10px}.c-article-share-box__button--link-like{background-color:transparent;border:0;color:#069;cursor:pointer;font-size:.875rem;margin-bottom:8px;margin-left:10px}.c-article-editorial-summary__container{font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:1rem}.c-article-editorial-summary__container .c-article-editorial-summary__content p:last-child{margin-bottom:0}.c-article-editorial-summary__container .c-article-editorial-summary__content--less{max-height:9.5rem;overflow:hidden}.c-article-editorial-summary__container .c-article-editorial-summary__button{background-color:#fff;border:0;color:#069;font-size:.875rem;margin-bottom:16px}.c-article-editorial-summary__container .c-article-editorial-summary__button.active,.c-article-editorial-summary__container .c-article-editorial-summary__button.hover,.c-article-editorial-summary__container .c-article-editorial-summary__button:active,.c-article-editorial-summary__container .c-article-editorial-summary__button:hover{text-decoration:underline;text-decoration-skip-ink:auto}.c-article-associated-content__container .c-article-associated-content__collection-label{font-size:.875rem;line-height:1.4}.c-article-associated-content__container .c-article-associated-content__collection-title{line-height:1.3}.c-context-bar{box-shadow:0 0 10px 0 rgba(51,51,51,.2);position:relative;width:100%}.c-context-bar__title{display:none}.c-reading-companion{clear:both;min-height:389px}.c-reading-companion__sticky{max-width:389px}.c-reading-companion__scroll-pane{margin:0;min-height:200px;overflow:hidden auto}.c-reading-companion__tabs{display:flex;flex-flow:row nowrap;font-size:1rem;list-style:none;margin:0 0 8px;padding:0}.c-reading-companion__tabs>li{flex-grow:1}.c-reading-companion__tab{background-color:#eee;border:1px solid #d5d5d5;border-image:initial;border-left-width:0;color:#069;font-size:1rem;padding:8px 8px 8px 15px;text-align:left;width:100%}.c-reading-companion__tabs li:first-child .c-reading-companion__tab{border-left-width:1px}.c-reading-companion__tab--active{background-color:#fff;border-bottom:1px solid #fff;color:#222;font-weight:700}.c-reading-companion__sections-list{list-style:none;padding:0}.c-reading-companion__figures-list,.c-reading-companion__references-list{list-style:none;min-height:389px;padding:0}.c-reading-companion__references-list--numeric{list-style:decimal inside}.c-reading-companion__sections-list{margin:0 0 8px;min-height:50px}.c-reading-companion__section-item{font-size:1rem;padding:0}.c-reading-companion__section-item a{display:block;line-height:1.5;overflow:hidden;padding:8px 0 8px 16px;text-overflow:ellipsis;white-space:nowrap}.c-reading-companion__figure-item{border-top:1px solid #d5d5d5;font-size:1rem;padding:16px 8px 16px 0}.c-reading-companion__figure-item:first-child{border-top:none;padding-top:8px}.c-reading-companion__reference-item{border-top:1px solid #d5d5d5;font-size:1rem;padding:8px 8px 8px 16px}.c-reading-companion__reference-item:first-child{border-top:none}.c-reading-companion__reference-item a{word-break:break-word}.c-reading-companion__reference-citation{display:inline}.c-reading-companion__reference-links{font-size:.813rem;font-weight:700;list-style:none;margin:8px 0 0;padding:0;text-align:right}.c-reading-companion__reference-links>a{display:inline-block;padding-left:8px}.c-reading-companion__reference-links>a:first-child{display:inline-block;padding-left:0}.c-reading-companion__figure-title{display:block;margin:0 0 8px}.c-reading-companion__figure-links{display:flex;justify-content:space-between;margin:8px 0 0}.c-reading-companion__figure-links>a{align-items:center;display:flex}.c-reading-companion__figure-full-link svg{height:.8em;margin-left:2px}.c-reading-companion__panel{border-top:none;display:none;margin-top:0;padding-top:0}.c-cod,.c-reading-companion__panel--active{display:block}.c-cod{font-size:1rem;width:100%}.c-cod__form{background:#ebf0f3}.c-cod__prompt{font-size:1.125rem;line-height:1.3;margin:0 0 24px}.c-cod__label{display:block;margin:0 0 4px}.c-cod__row{display:flex;margin:0 0 16px}.c-cod__row:last-child{margin:0}.c-cod__input{border:1px solid #d5d5d5;border-radius:2px;flex-basis:75%;flex-shrink:0;margin:0;padding:13px}.c-cod__input--submit{background-color:#069;border:1px solid #069;color:#fff;flex-shrink:1;margin-left:8px;transition:background-color .2s ease-out 0s,color .2s ease-out 0s}.c-cod__input--submit-single{flex-basis:100%;flex-shrink:0;margin:0}.c-cod__input--submit:focus,.c-cod__input--submit:hover{background-color:#fff;color:#069}.c-pdf-download__link .u-icon{padding-top:2px}.c-pdf-download{display:flex;margin-bottom:16px;max-height:48px}@media only screen and (min-width:540px){.c-pdf-download{max-height:none}}@media only screen and (min-width:1024px){.c-pdf-download{max-height:48px}}.c-pdf-download__link{display:flex;flex:1 1 0%}.c-pdf-download__link:hover{text-decoration:none}.c-pdf-download__text{padding-right:4px}@media only screen and (max-width:539px){.c-pdf-download__text{text-transform:capitalize}}@media only screen and (min-width:540px){.c-pdf-download__text{padding-right:8px}}.c-context-bar--sticky .c-pdf-download{display:block;margin-bottom:0;white-space:nowrap}@media only screen and (max-width:539px){.c-pdf-download .u-sticky-visually-hidden{clip:rect(0,0,0,0);border:0;height:1px;margin:-100%;overflow:hidden;padding:0;position:absolute!important;width:1px}}.c-pdf-container{display:flex;justify-content:flex-end}@media only screen and (max-width:539px){.c-pdf-container .c-pdf-download{display:flex;flex-basis:100%}}.c-pdf-container .c-pdf-download+.c-pdf-download{margin-left:16px}.c-article-extras .c-pdf-container .c-pdf-download{width:100%}.c-article-extras .c-pdf-container .c-pdf-download+.c-pdf-download{margin-left:0}@media only screen and (min-width:540px){.c-context-bar--sticky .c-pdf-download__link{align-items:center;flex:1 1 183px}}@media only screen and (max-width:320px){.c-context-bar--sticky .c-pdf-download__link{padding:16px}}.article-page--commercial .c-article-main-column .c-pdf-button__container .c-pdf-download{display:none}@media only screen and (max-width:1023px){.article-page--commercial .c-article-main-column .c-pdf-button__container .c-pdf-download{display:block}}.c-status-message--success{border-bottom:2px solid #00b8b0;justify-content:center;margin-bottom:16px;padding-bottom:8px}.c-recommendations-list__item .c-card{flex-basis:100%}.c-recommendations-list__item .c-card__image{align-items:baseline;flex:1 1 40%;margin:0 0 0 16px;max-width:150px}.c-recommendations-list__item .c-card__image img{border:1px solid #cedbe0;height:auto;min-height:0;position:static}@media only screen and (max-width:1023px){.c-recommendations-list__item .c-card__image{display:none}}.c-card__layout{display:flex;flex:1 1 auto;justify-content:space-between}.c-card__title-recommendation{-webkit-box-orient:vertical;-webkit-line-clamp:4;display:-webkit-box;font-size:1rem;font-weight:700;line-height:1.4;margin:0 0 8px;max-height:5.6em;overflow:hidden!important;text-overflow:ellipsis}.c-card__title-recommendation .c-card__link{color:inherit}.c-card__title-recommendation .c-card__link:hover{text-decoration:underline}.c-card__title-recommendation .MathJax_Display{display:inline!important}.c-card__link:not(.c-card__link--no-block-link):before{z-index:1}.c-article-metrics__heading a,.c-article-metrics__posts .c-card__title a,.c-article-recommendations-card__link{color:inherit}.c-recommendations-column-switch .c-meta{margin-top:auto}.c-article-recommendations-card__meta-type,.c-meta .c-meta__item:first-child{font-weight:700}.c-article-body .c-article-recommendations-card__authors{display:none;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:.875rem;line-height:1.5;margin:0 0 8px}@media only screen and (max-width:539px){.c-article-body .c-article-recommendations-card__authors{display:block;margin:0}}.c-article-metrics__posts .c-card__title{font-size:1.05rem}.c-article-metrics__posts .c-card__title+span{color:#6f6f6f;font-size:1rem}p{overflow-wrap:break-word;word-break:break-word}.c-ad{text-align:center}@media only screen and (min-width:320px){.c-ad{padding:8px}}.c-ad--728x90{background-color:#ccc;display:none}.c-ad--728x90 .c-ad__inner{min-height:calc(1.5em + 94px)}@media only screen and (min-width:768px){.js .c-ad--728x90{display:none}}.c-ad__label{color:#333;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:.875rem;font-weight:400;line-height:1.5;margin-bottom:4px}.c-author-list{color:#6f6f6f;font-family:inherit;font-size:1rem;line-height:inherit;list-style:none;margin:0;padding:0}.c-author-list>li,.c-breadcrumbs>li,.c-footer__links>li,.js .c-author-list,.u-list-comma-separated>li,.u-list-inline>li{display:inline}.c-author-list>li:not(:first-child):not(:last-child):before{content:", "}.c-author-list>li:not(:only-child):last-child:before{content:" & "}.c-author-list--compact{font-size:.875rem;line-height:1.4}.c-author-list--truncated>li:not(:only-child):last-child:before{content:" ... "}.js .c-author-list__hide{display:none;visibility:hidden}.js .c-author-list__hide:first-child+*{margin-block-start:0}.c-meta{color:inherit;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:.875rem;line-height:1.4;list-style:none;margin:0;padding:0}.c-meta--large{font-size:1rem}.c-meta--large .c-meta__item{margin-bottom:8px}.c-meta__item{display:inline-block;margin-bottom:4px}.c-meta__item:not(:last-child){border-right:1px solid #d5d5d5;margin-right:4px;padding-right:4px}@media only screen and (max-width:539px){.c-meta__item--block-sm-max{display:block}.c-meta__item--block-sm-max:not(:last-child){border-right:none;margin-right:0;padding-right:0}}@media only screen and (min-width:1024px){.c-meta__item--block-at-lg{display:block}.c-meta__item--block-at-lg:not(:last-child){border-right:none;margin-right:0;padding-right:0}}.c-meta__type{font-weight:700;text-transform:none}.c-skip-link{background:#069;bottom:auto;color:#fff;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:.875rem;padding:8px;position:absolute;text-align:center;transform:translateY(-100%);z-index:9999}@media (prefers-reduced-motion:reduce){.c-skip-link{transition:top .3s ease-in-out 0s}}@media print{.c-skip-link{display:none}}.c-skip-link:link{color:#fff}.c-status-message{align-items:center;box-sizing:border-box;display:flex;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:1rem;position:relative;width:100%}.c-card__summary>p:last-child,.c-status-message :last-child{margin-bottom:0}.c-status-message--boxed{background-color:#fff;border:1px solid #eee;border-radius:2px;line-height:1.4;padding:16px}.c-status-message__heading{font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:1rem;font-weight:700}.c-status-message__icon{fill:currentcolor;display:inline-block;flex:0 0 auto;height:1.5em;margin-right:8px;transform:translate(0);vertical-align:text-top;width:1.5em}.c-status-message__icon--top{align-self:flex-start}.c-status-message--info .c-status-message__icon{color:#003f8d}.c-status-message--boxed.c-status-message--info{border-bottom:4px solid #003f8d}.c-status-message--error .c-status-message__icon{color:#c40606}.c-status-message--boxed.c-status-message--error{border-bottom:4px solid #c40606}.c-status-message--success .c-status-message__icon{color:#00b8b0}.c-status-message--boxed.c-status-message--success{border-bottom:4px solid #00b8b0}.c-status-message--warning .c-status-message__icon{color:#edbc53}.c-status-message--boxed.c-status-message--warning{border-bottom:4px solid #edbc53}.c-breadcrumbs{color:#000;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:1rem;list-style:none;margin:0;padding:0}.c-breadcrumbs__link{color:#666}svg.c-breadcrumbs__chevron{fill:#888;height:10px;margin:4px 4px 0;width:10px}@media only screen and (max-width:539px){.c-breadcrumbs .c-breadcrumbs__item{display:none}.c-breadcrumbs .c-breadcrumbs__item:last-child,.c-breadcrumbs .c-breadcrumbs__item:nth-last-child(2){display:inline}}.c-card{background-color:transparent;border:0;box-shadow:none;display:flex;flex-direction:column;font-size:14px;min-width:0;overflow:hidden;padding:0;position:relative}.c-card--no-shape{background:0 0;border:0;box-shadow:none}.c-card__image{display:flex;justify-content:center;overflow:hidden;padding-bottom:56.25%;position:relative}@supports (aspect-ratio:1/1){.c-card__image{padding-bottom:0}}.c-card__image img{left:0;min-height:100%;min-width:100%;position:absolute}@supports ((-o-object-fit:cover) or (object-fit:cover)){.c-card__image img{height:100%;object-fit:cover;width:100%}}.c-card__body{flex:1 1 auto;padding:16px}.c-card--no-shape .c-card__body{padding:0}.c-card--no-shape .c-card__body:not(:first-child){padding-top:16px}.c-card__title{letter-spacing:-.01875rem;margin-bottom:8px;margin-top:0}[lang=de] .c-card__title{hyphens:auto}.c-card__summary{line-height:1.4}.c-card__summary>p{margin-bottom:5px}.c-card__summary a{text-decoration:underline}.c-card__link:not(.c-card__link--no-block-link):before{bottom:0;content:"";left:0;position:absolute;right:0;top:0}.c-card--flush .c-card__body{padding:0}.c-card--major{font-size:1rem}.c-card--dark{background-color:#29303c;border-width:0;color:#e3e4e5}.c-card--dark .c-card__title{color:#fff}.c-card--dark .c-card__link,.c-card--dark .c-card__summary a{color:inherit}.c-header{background-color:#fff;border-bottom:5px solid #000;font-size:1rem;line-height:1.4;margin-bottom:16px}.c-header__row{padding:0;position:relative}.c-header__row:not(:last-child){border-bottom:1px solid #eee}.c-header__split{align-items:center;display:flex;justify-content:space-between}.c-header__logo-container{flex:1 1 0px;line-height:0;margin:8px 24px 8px 0}.c-header__logo{transform:translateZ(0)}.c-header__logo img{max-height:32px}.c-header__container{margin:0 auto;max-width:1280px}.c-header__menu{align-items:center;display:flex;flex:0 1 auto;flex-wrap:wrap;font-weight:700;gap:8px 8px;line-height:1.4;list-style:none;margin:0 -8px;padding:0}@media print{.c-header__menu{display:none}}@media only screen and (max-width:1023px){.c-header__menu--hide-lg-max{display:none;visibility:hidden}}.c-header__menu--global{font-weight:400;justify-content:flex-end}.c-header__menu--global svg{display:none;visibility:hidden}.c-header__menu--global svg:first-child+*{margin-block-start:0}@media only screen and (min-width:540px){.c-header__menu--global svg{display:block;visibility:visible}}.c-header__menu--journal{font-size:.875rem;margin:8px 0 8px -8px}@media only screen and (min-width:540px){.c-header__menu--journal{flex-wrap:nowrap;font-size:1rem}}.c-header__item{padding-bottom:0;padding-top:0;position:static}.c-header__item--pipe{border-left:2px solid #eee;padding-left:8px}.c-header__item--padding{padding-bottom:8px;padding-top:8px}@media only screen and (min-width:540px){.c-header__item--dropdown-menu{position:relative}}@media only screen and (min-width:1024px){.c-header__item--hide-lg{display:none;visibility:hidden}}@media only screen and (max-width:767px){.c-header__item--hide-md-max{display:none;visibility:hidden}.c-header__item--hide-md-max:first-child+*{margin-block-start:0}}.c-header__link{align-items:center;color:inherit;display:inline-flex;gap:4px 4px;padding:8px;white-space:nowrap}.c-header__link svg{transition-duration:.2s}.c-header__show-text{display:none;visibility:hidden}.has-tethered .c-header__heading--js-hide:first-child+*{margin-block-start:0}@media only screen and (min-width:540px){.c-header__show-text{display:inline;visibility:visible}}.c-header__dropdown{background-color:#000;border-bottom:1px solid #2f2f2f;color:#eee;font-size:.875rem;line-height:1.2;padding:16px 0}@media print{.c-header__dropdown{display:none}}.c-header__heading{display:inline-block;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:1.25rem;font-weight:400;line-height:1.4;margin-bottom:8px}.c-header__heading--keyline{border-top:1px solid;border-color:#2f2f2f;margin-top:16px;padding-top:16px;width:100%}.c-header__list{display:flex;flex-wrap:wrap;gap:0 16px;list-style:none;margin:0 -8px}.c-header__flush{margin:0 -8px}.c-header__visually-hidden{clip:rect(0,0,0,0);border:0;height:1px;margin:-100%;overflow:hidden;padding:0;position:absolute!important;width:1px}.c-header__search-form{margin-bottom:8px}.c-header__search-layout{display:flex;flex-wrap:wrap;gap:16px 16px}.c-header__search-layout>:first-child{flex:999 1 auto}.c-header__search-layout>*{flex:1 1 auto}.c-header__search-layout--max-width{max-width:720px}.c-header__search-button{align-items:center;background-color:transparent;background-image:none;border:1px solid #fff;border-radius:2px;color:#fff;cursor:pointer;display:flex;font-family:sans-serif;font-size:1rem;justify-content:center;line-height:1.15;margin:0;padding:8px 16px;position:relative;text-decoration:none;transition:all .25s ease 0s,color .25s ease 0s,border-color .25s ease 0s;width:100%}.u-button svg,.u-button--primary svg{fill:currentcolor}.c-header__input,.c-header__select{border:1px solid;border-radius:3px;box-sizing:border-box;font-size:1rem;padding:8px 16px;width:100%}.c-header__select{-webkit-appearance:none;background-image:url("data:image/svg+xml,%3Csvg height='16' viewBox='0 0 16 16' width='16' xmlns='http://www.w3.org/2000/svg'%3E%3Cpath d='m5.58578644 3-3.29289322-3.29289322c-.39052429-.39052429-.39052429-1.02368927 0-1.41421356s1.02368927-.39052429 1.41421356 0l4 4c.39052429.39052429.39052429 1.02368927 0 1.41421356l-4 4c-.39052429.39052429-1.02368927.39052429-1.41421356 0s-.39052429-1.02368927 0-1.41421356z' fill='%23333' fill-rule='evenodd' transform='matrix(0 1 -1 0 11 3)'/%3E%3C/svg%3E");background-position:right .7em top 50%;background-repeat:no-repeat;background-size:1em;box-shadow:0 1px 0 1px rgba(0,0,0,.04);display:block;margin:0;max-width:100%;min-width:150px}@media only screen and (min-width:540px){.c-header__menu--journal .c-header__item--dropdown-menu:last-child .c-header__dropdown.has-tethered{left:auto;right:0}}@media only screen and (min-width:768px){.c-header__menu--journal .c-header__item--dropdown-menu:last-child .c-header__dropdown.has-tethered{left:0;right:auto}}.c-header__dropdown.has-tethered{border-bottom:0;border-radius:0 0 2px 2px;left:0;position:absolute;top:100%;transform:translateY(5px);width:100%;z-index:1}@media only screen and (min-width:540px){.c-header__dropdown.has-tethered{transform:translateY(8px);width:auto}}@media only screen and (min-width:768px){.c-header__dropdown.has-tethered{min-width:225px}}.c-header__dropdown--full-width.has-tethered{padding:32px 0 24px;transform:none;width:100%}.has-tethered .c-header__heading--js-hide{display:none;visibility:hidden}.has-tethered .c-header__list--js-stack{flex-direction:column}.has-tethered .c-header__item--keyline,.has-tethered .c-header__list~.c-header__list .c-header__item:first-child{border-top:1px solid #d5d5d5;margin-top:8px;padding-top:8px}.c-header__item--snid-account-widget{display:flex}.c-header__container{padding:0 4px}.c-header__list{padding:0 12px}.c-header__menu .c-header__link{font-size:14px}.c-header__item--snid-account-widget .c-header__link{padding:8px}.c-header__menu--journal{margin-left:0}@media only screen and (min-width:540px){.c-header__container{padding:0 16px}.c-header__menu--journal{margin-left:-8px}.c-header__menu .c-header__link{font-size:16px}.c-header__link--search{gap:13px 13px}}.u-button{align-items:center;background-color:transparent;background-image:none;border:1px solid #069;border-radius:2px;color:#069;cursor:pointer;display:inline-flex;font-family:sans-serif;font-size:1rem;justify-content:center;line-height:1.3;margin:0;padding:8px;position:relative;text-decoration:none;transition:all .25s ease 0s,color .25s ease 0s,border-color .25s ease 0s;width:auto}.u-button--primary{background-color:#069;background-image:none;border:1px solid #069;color:#fff}.u-button--full-width{display:flex;width:100%}.u-display-none{display:none}.js .u-js-hide,.u-hide{display:none;visibility:hidden}.u-hide:first-child+*{margin-block-start:0}.u-visually-hidden{clip:rect(0,0,0,0);border:0;height:1px;margin:-100%;overflow:hidden;padding:0;position:absolute!important;width:1px}@media print{.u-hide-print{display:none}}@media only screen and (min-width:1024px){.u-hide-at-lg{display:none;visibility:hidden}.u-hide-at-lg:first-child+*{margin-block-start:0}}.u-clearfix:after,.u-clearfix:before{content:"";display:table}.u-clearfix:after{clear:both}.u-color-open-access{color:#b74616}.u-float-left{float:left}.u-icon{fill:currentcolor;display:inline-block;height:1em;transform:translate(0);vertical-align:text-top;width:1em}.u-full-height{height:100%}.u-list-reset{list-style:none;margin:0;padding:0}.u-sans-serif{font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif}.u-container{margin:0 auto;max-width:1280px;padding:0 16px}.u-justify-content-space-between{justify-content:space-between}.u-mt-32{margin-top:32px}.u-mb-8{margin-bottom:8px}.u-mb-16{margin-bottom:16px}.u-mb-24{margin-bottom:24px}.u-mb-32{margin-bottom:32px}.c-nature-box svg+.c-article__button-text,.u-ml-8{margin-left:8px}.u-pa-16{padding:16px}html *,html :after,html :before{box-sizing:inherit}.c-article-section__title,.c-article-title{font-weight:700}.c-card__title{line-height:1.4em}.c-article__button{background-color:#069;border:1px solid #069;border-radius:2px;color:#fff;display:flex;font-family:-apple-system,BlinkMacSystemFont,Segoe UI,Roboto,Oxygen-Sans,Ubuntu,Cantarell,Helvetica Neue,sans-serif;font-size:.875rem;line-height:1.4;margin-bottom:16px;padding:13px;transition:background-color .2s ease-out 0s,color .2s ease-out 0s}.c-article__button,.c-article__button:hover{text-decoration:none}.c-article__button--inverted,.c-article__button:hover{background-color:#fff;color:#069}.c-article__button--inverted:hover{background-color:#069;color:#fff}.c-header__link{text-decoration:inherit}.grade-c-hide{display:block}.u-lazy-ad-wrapper{background-color:#ccc;display:none;min-height:137px}@media only screen and (min-width:768px){.u-lazy-ad-wrapper{display:block}}.c-nature-box{background-color:#fff;border:1px solid #d5d5d5;border-radius:2px;box-shadow:0 0 5px 0 rgba(51,51,51,.1);line-height:1.3;margin-bottom:24px;padding:16px 16px 3px}.c-nature-box__text{font-size:1rem;margin-bottom:16px}.c-nature-box .c-pdf-download{margin-bottom:16px!important}.c-nature-box--version{background-color:#eee}.c-nature-box__wrapper{transform:translateZ(0)}.c-nature-box__wrapper--placeholder{min-height:165px}.c-pdf-download__link{padding:13px 24px} } </style> <link data-test="critical-css-handler" data-inline-css-source="critical-css" rel="stylesheet" href="/static/css/enhanced-article-nature-branded-68c4876c28.css" media="print" onload="this.media='only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark)';this.onload=null"> <noscript> <link rel="stylesheet" type="text/css" href="/static/css/enhanced-article-nature-branded-68c4876c28.css" media="only print, only all and (prefers-color-scheme: no-preference), only all and (prefers-color-scheme: light), only all and (prefers-color-scheme: dark)"> </noscript> <link rel="stylesheet" type="text/css" href="/static/css/article-print-122346e276.css" media="print"> <link rel="apple-touch-icon" sizes="180x180" href=/static/images/favicons/nature/apple-touch-icon-f39cb19454.png> <link rel="icon" type="image/png" sizes="48x48" href=/static/images/favicons/nature/favicon-48x48-b52890008c.png> <link rel="icon" type="image/png" sizes="32x32" href=/static/images/favicons/nature/favicon-32x32-3fe59ece92.png> <link rel="icon" type="image/png" sizes="16x16" href=/static/images/favicons/nature/favicon-16x16-951651ab72.png> <link rel="manifest" href=/static/manifest.json crossorigin="use-credentials"> <link rel="mask-icon" href=/static/images/favicons/nature/safari-pinned-tab-69bff48fe6.svg color="#000000"> <link rel="shortcut icon" href=/static/images/favicons/nature/favicon.ico> <meta name="msapplication-TileColor" content="#000000"> <meta name="msapplication-config" content=/static/browserconfig.xml> <meta name="theme-color" content="#000000"> <meta name="application-name" content="Nature"> <script> (function () { if ( typeof window.CustomEvent === "function" ) return false; function CustomEvent ( event, params ) { params = params || { bubbles: false, cancelable: false, detail: null }; var evt = document.createEvent( 'CustomEvent' ); evt.initCustomEvent( event, params.bubbles, params.cancelable, params.detail ); return evt; } CustomEvent.prototype = window.Event.prototype; window.CustomEvent = CustomEvent; })(); </script>  <script data-test="gtm-head"> window.initGTM = function() { if (window.config.mustardcut) { (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push({'gtm.start': new Date().getTime(), event: 'gtm.js'}); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? '&l=' + l : ''; j.async = true; j.src = 'https://www.googletagmanager.com/gtm.js?id=' + i + dl; f.parentNode.insertBefore(j, f); })(window, document, 'script', 'dataLayer', 'GTM-MRVXSHQ'); } } </script>  <script> (function(w,d,t) { function cc() { var h = w.location.hostname; if (h.indexOf('preview-www.nature.com') > -1) return; var e = d.createElement(t), s = d.getElementsByTagName(t)[0]; if (h.indexOf('nature.com') > -1) { if (h.indexOf('test-www.nature.com') > -1) { e.src = 'https://cmp.nature.com/production_live/en/consent-bundle-8-68.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } else { e.src = 'https://cmp.nature.com/production_live/en/consent-bundle-8-68.js'; e.setAttribute('onload', "initGTM(window,document,'script','dataLayer','GTM-MRVXSHQ')"); } } else { e.src = '/static/js/cookie-consent-es5-bundle-cb57c2c98a.js'; e.setAttribute('data-consent', h); } s.insertAdjacentElement('afterend', e); } cc(); })(window,document,'script'); </script> <script id="js-position0"> (function(w, d) { w.idpVerifyPrefix = 'https://verify.nature.com'; w.ra21Host = 'https://wayf.springernature.com'; var moduleSupport = (function() { return 'noModule' in d.createElement('script'); })(); if (w.config.mustardcut === true) { w.loader = { index: 0, registered: [], scripts: [ {src: '/static/js/global-article-es6-bundle-c8a573ca90.js', test: 'global-article-js', module: true}, {src: '/static/js/global-article-es5-bundle-d17603b9e9.js', test: 'global-article-js', nomodule: true}, {src: '/static/js/shared-es6-bundle-606cb67187.js', test: 'shared-js', module: true}, {src: '/static/js/shared-es5-bundle-e919764a53.js', test: 'shared-js', nomodule: true}, {src: '/static/js/header-150-es6-bundle-5bb959eaa1.js', test: 'header-150-js', module: true}, {src: '/static/js/header-150-es5-bundle-994fde5b1d.js', test: 'header-150-js', nomodule: true} ].filter(function (s) { if (s.src === null) return false; if (moduleSupport && s.nomodule) return false; return !(!moduleSupport && s.module); }), register: function (value) { this.registered.push(value); }, ready: function () { if (this.registered.length === this.scripts.length) { this.registered.forEach(function (fn) { if (typeof fn === 'function') { setTimeout(fn, 0); } }); this.ready = function () {}; } }, insert: function (s) { var t = d.getElementById('js-position' + this.index); if (t && t.insertAdjacentElement) { t.insertAdjacentElement('afterend', s); } else { d.head.appendChild(s); } ++this.index; }, createScript: function (script, beforeLoad) { var s = d.createElement('script'); s.id = 'js-position' + (this.index + 1); s.setAttribute('data-test', script.test); if (beforeLoad) { s.defer = 'defer'; s.onload = function () { if (script.noinit) { loader.register(true); } if (d.readyState === 'interactive' || d.readyState === 'complete') { loader.ready(); } }; } else { s.async = 'async'; } s.src = script.src; return s; }, init: function () { this.scripts.forEach(function (s) { loader.insert(loader.createScript(s, true)); }); d.addEventListener('DOMContentLoaded', function () { loader.ready(); var conditionalScripts; conditionalScripts = [ {match: 'div[data-pan-container]', src: '/static/js/pan-zoom-es6-bundle-464a2af269.js', test: 'pan-zoom-js', module: true }, {match: 'div[data-pan-container]', src: '/static/js/pan-zoom-es5-bundle-98fb9b653b.js', test: 'pan-zoom-js', nomodule: true }, {match: 'math,span.mathjax-tex', src: '/static/js/math-es6-bundle-23597ae350.js', test: 'math-js', module: true}, {match: 'math,span.mathjax-tex', src: '/static/js/math-es5-bundle-6532c6f78b.js', test: 'math-js', nomodule: true} ]; if (conditionalScripts) { conditionalScripts.filter(function (script) { return !!document.querySelector(script.match) && !((moduleSupport && script.nomodule) || (!moduleSupport && script.module)); }).forEach(function (script) { loader.insert(loader.createScript(script)); }); } }, false); } }; loader.init(); } })(window, document); </script> <meta name="robots" content="noarchive"> <meta name="access" content="Yes"> <link rel="search" href="https://www.nature.com/search"> <link rel="search" href="https://www.nature.com/opensearch/opensearch.xml" type="application/opensearchdescription+xml" title="nature.com"> <link rel="search" href="https://www.nature.com/opensearch/request" type="application/sru+xml" title="nature.com"> <script type="application/ld+json">{"mainEntity":{"headline":"Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor","description":"Despite the increasing number of GPCR structures and recent advances in peptide design, the development of efficient technologies allowing rational design of high-affinity peptide ligands for single GPCRs remains an unmet challenge. Here, we develop a computational approach for designing conjugates of lariat-shaped macrocyclized peptides and a small molecule opioid ligand. We demonstrate its feasibility by discovering chemical scaffolds for the kappa-opioid receptor (KOR) with desired pharmacological activities. The designed De Novo Cyclic Peptide (DNCP)-β-naloxamine (NalA) exhibit in vitro potent mixed KOR agonism/mu-opioid receptor (MOR) antagonism, nanomolar binding affinity, selectivity, and efficacy bias at KOR. Proof-of-concept in vivo efficacy studies demonstrate that DNCP-β-NalA(1) induces a potent KOR-mediated antinociception in male mice. The high-resolution cryo-EM structure (2.6 Å) of the DNCP-β-NalA–KOR–Gi1 complex and molecular dynamics simulations are harnessed to validate the computational design model. This reveals a network of residues in ECL2/3 and TM6/7 controlling the intrinsic efficacy of KOR. In general, our computational de novo platform overcomes extensive lead optimization encountered in ultra-large library docking and virtual small molecule screening campaigns and offers innovation for GPCR ligand discovery. This may drive the development of next-generation therapeutics for medical applications such as pain conditions. Despite advances in GPCR structures and peptide design, creating high-affinity ligands remains a challenge. Here the authors develop a computational method, successfully identifying peptide-based molecules for KOR: their platform shows promise for streamlined GPCR ligand discovery.","datePublished":"2023-12-06T00:00:00Z","dateModified":"2023-12-06T00:00:00Z","pageStart":"1","pageEnd":"17","license":"http://creativecommons.org/licenses/by/4.0/","sameAs":"https://doi.org/10.1038/s41467-023-43718-w","keywords":["Peptides","Protein design","Receptor pharmacology","Science","Humanities and Social Sciences","multidisciplinary"],"image":["https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig1_HTML.png","https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig2_HTML.png","https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig3_HTML.png","https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig4_HTML.png","https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig5_HTML.png"],"isPartOf":{"name":"Nature Communications","issn":["2041-1723"],"volumeNumber":"14","@type":["Periodical","PublicationVolume"]},"publisher":{"name":"Nature Publishing Group UK","logo":{"url":"https://www.springernature.com/app-sn/public/images/logo-springernature.png","@type":"ImageObject"},"@type":"Organization"},"author":[{"name":"Edin Muratspahić","affiliation":[{"name":"Medical University of Vienna","address":{"name":"Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria","@type":"PostalAddress"},"@type":"Organization"},{"name":"University of Washington","address":{"name":"Institute for Protein Design, University of Washington, Seattle, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Kristine Deibler","url":"http://orcid.org/0000-0002-2376-8456","affiliation":[{"name":"University of Washington","address":{"name":"Institute for Protein Design, University of Washington, Seattle, USA","@type":"PostalAddress"},"@type":"Organization"},{"name":"Novo Nordisk A/S","address":{"name":"Novo Nordisk Research Center Seattle, Novo Nordisk A/S, Seattle, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Jianming Han","affiliation":[{"name":"University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine","address":{"name":"Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Nataša Tomašević","affiliation":[{"name":"Medical University of Vienna","address":{"name":"Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Kirtikumar B. Jadhav","affiliation":[{"name":"University of Vienna","address":{"name":"Institute of Biological Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Aina-Leonor Olivé-Marti","affiliation":[{"name":"University of Innsbruck","address":{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmacy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Nadine Hochrainer","affiliation":[{"name":"University of Innsbruck","address":{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmacy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Roland Hellinger","affiliation":[{"name":"Medical University of Vienna","address":{"name":"Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Johannes Koehbach","affiliation":[{"name":"The University of Queensland","address":{"name":"Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Australia","@type":"PostalAddress"},"@type":"Organization"},{"name":"The University of Queensland","address":{"name":"School of Biomedical Sciences, Faculty for Medicine, The University of Queensland, Brisbane, Australia","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Jonathan F. Fay","url":"http://orcid.org/0000-0003-1822-2384","affiliation":[{"name":"University of Maryland Baltimore","address":{"name":"Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Mohammad Homaidur Rahman","affiliation":[{"name":"University of Health Sciences & Pharmacy in St. Louis","address":{"name":"Department of Pharmaceutical and Administrative Sciences, Saint Louis College of Pharmacy, University of Health Sciences & Pharmacy in St. Louis, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Lamees Hegazy","affiliation":[{"name":"University of Health Sciences & Pharmacy in St. Louis","address":{"name":"Department of Pharmaceutical and Administrative Sciences, Saint Louis College of Pharmacy, University of Health Sciences & Pharmacy in St. Louis, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Timothy W. Craven","affiliation":[{"name":"University of Washington","address":{"name":"Institute for Protein Design, University of Washington, Seattle, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Balazs R. Varga","affiliation":[{"name":"University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine","address":{"name":"Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"},{"name":"Washington University School of Medicine","address":{"name":"Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Gaurav Bhardwaj","affiliation":[{"name":"University of Washington","address":{"name":"Institute for Protein Design, University of Washington, Seattle, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Kevin Appourchaux","url":"http://orcid.org/0000-0001-9853-3347","affiliation":[{"name":"University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine","address":{"name":"Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"},{"name":"Washington University School of Medicine","address":{"name":"Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Susruta Majumdar","url":"http://orcid.org/0000-0002-2931-3823","affiliation":[{"name":"University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine","address":{"name":"Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"},{"name":"Washington University School of Medicine","address":{"name":"Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Markus Muttenthaler","affiliation":[{"name":"University of Vienna","address":{"name":"Institute of Biological Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria","@type":"PostalAddress"},"@type":"Organization"},{"name":"The University of Queensland","address":{"name":"Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Parisa Hosseinzadeh","url":"http://orcid.org/0000-0002-3128-7433","affiliation":[{"name":"University of Oregon","address":{"name":"Department of Bioengineering, Knight Campus, University of Oregon, Eugene, USA","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"David J. Craik","url":"http://orcid.org/0000-0003-0007-6796","affiliation":[{"name":"The University of Queensland","address":{"name":"Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Australia","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Mariana Spetea","url":"http://orcid.org/0000-0002-2379-5358","affiliation":[{"name":"University of Innsbruck","address":{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmacy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria","@type":"PostalAddress"},"@type":"Organization"}],"@type":"Person"},{"name":"Tao Che","url":"http://orcid.org/0000-0002-1620-3027","affiliation":[{"name":"University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine","address":{"name":"Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"},{"name":"Washington University School of Medicine","address":{"name":"Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA","@type":"PostalAddress"},"@type":"Organization"}],"email":"taoche@wustl.edu","@type":"Person"},{"name":"David Baker","url":"http://orcid.org/0000-0001-7896-6217","affiliation":[{"name":"University of Washington","address":{"name":"Institute for Protein Design, University of Washington, Seattle, USA","@type":"PostalAddress"},"@type":"Organization"},{"name":"University of Washington","address":{"name":"Department of Biochemistry, University of Washington, Seattle, USA","@type":"PostalAddress"},"@type":"Organization"},{"name":"University of Washington, Seattle","address":{"name":"Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA","@type":"PostalAddress"},"@type":"Organization"}],"email":"dabaker@uw.edu","@type":"Person"},{"name":"Christian W. Gruber","url":"http://orcid.org/0000-0001-6060-7048","affiliation":[{"name":"Medical University of Vienna","address":{"name":"Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria","@type":"PostalAddress"},"@type":"Organization"}],"email":"christian.w.gruber@meduniwien.ac.at","@type":"Person"}],"isAccessibleForFree":true,"@type":"ScholarlyArticle"},"@context":"https://schema.org","@type":"WebPage"}</script> <link rel="canonical" href="https://www.nature.com/articles/s41467-023-43718-w"> <meta name="journal_id" content="41467"/> <meta name="dc.title" content="Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor"/> <meta name="dc.source" content="Nature Communications 2023 14:1"/> <meta name="dc.format" content="text/html"/> <meta name="dc.publisher" content="Nature Publishing Group"/> <meta name="dc.date" content="2023-12-06"/> <meta name="dc.type" content="OriginalPaper"/> <meta name="dc.language" content="En"/> <meta name="dc.copyright" content="2023 The Author(s)"/> <meta name="dc.rights" content="2023 The Author(s)"/> <meta name="dc.rightsAgent" content="journalpermissions@springernature.com"/> <meta name="dc.description" content="Despite the increasing number of GPCR structures and recent advances in peptide design, the development of efficient technologies allowing rational design of high-affinity peptide ligands for single GPCRs remains an unmet challenge. Here, we develop a computational approach for designing conjugates of lariat-shaped macrocyclized peptides and a small molecule opioid ligand. We demonstrate its feasibility by discovering chemical scaffolds for the kappa-opioid receptor (KOR) with desired pharmacological activities. The designed De Novo Cyclic Peptide (DNCP)-β-naloxamine (NalA) exhibit in vitro potent mixed KOR agonism/mu-opioid receptor (MOR) antagonism, nanomolar binding affinity, selectivity, and efficacy bias at KOR. Proof-of-concept in vivo efficacy studies demonstrate that DNCP-β-NalA(1) induces a potent KOR-mediated antinociception in male mice. The high-resolution cryo-EM structure (2.6 Å) of the DNCP-β-NalA–KOR–Gi1 complex and molecular dynamics simulations are harnessed to validate the computational design model. This reveals a network of residues in ECL2/3 and TM6/7 controlling the intrinsic efficacy of KOR. In general, our computational de novo platform overcomes extensive lead optimization encountered in ultra-large library docking and virtual small molecule screening campaigns and offers innovation for GPCR ligand discovery. This may drive the development of next-generation therapeutics for medical applications such as pain conditions. Despite advances in GPCR structures and peptide design, creating high-affinity ligands remains a challenge. Here the authors develop a computational method, successfully identifying peptide-based molecules for KOR: their platform shows promise for streamlined GPCR ligand discovery."/> <meta name="prism.issn" content="2041-1723"/> <meta name="prism.publicationName" content="Nature Communications"/> <meta name="prism.publicationDate" content="2023-12-06"/> <meta name="prism.volume" content="14"/> <meta name="prism.number" content="1"/> <meta name="prism.section" content="OriginalPaper"/> <meta name="prism.startingPage" content="1"/> <meta name="prism.endingPage" content="17"/> <meta name="prism.copyright" content="2023 The Author(s)"/> <meta name="prism.rightsAgent" content="journalpermissions@springernature.com"/> <meta name="prism.url" content="https://www.nature.com/articles/s41467-023-43718-w"/> <meta name="prism.doi" content="doi:10.1038/s41467-023-43718-w"/> <meta name="citation_pdf_url" content="https://www.nature.com/articles/s41467-023-43718-w.pdf"/> <meta name="citation_fulltext_html_url" content="https://www.nature.com/articles/s41467-023-43718-w"/> <meta name="citation_journal_title" content="Nature Communications"/> <meta name="citation_journal_abbrev" content="Nat Commun"/> <meta name="citation_publisher" content="Nature Publishing Group"/> <meta name="citation_issn" content="2041-1723"/> <meta name="citation_title" content="Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor"/> <meta name="citation_volume" content="14"/> <meta name="citation_issue" content="1"/> <meta name="citation_online_date" content="2023/12/06"/> <meta name="citation_firstpage" content="1"/> <meta name="citation_lastpage" content="17"/> <meta name="citation_article_type" content="Article"/> <meta name="citation_fulltext_world_readable" content=""/> <meta name="citation_language" content="en"/> <meta name="dc.identifier" content="doi:10.1038/s41467-023-43718-w"/> <meta name="DOI" content="10.1038/s41467-023-43718-w"/> <meta name="size" content="298827"/> <meta name="citation_doi" content="10.1038/s41467-023-43718-w"/> <meta name="citation_springer_api_url" content="http://api.springer.com/xmldata/jats?q=doi:10.1038/s41467-023-43718-w&api_key="/> <meta name="description" content="Despite the increasing number of GPCR structures and recent advances in peptide design, the development of efficient technologies allowing rational design of high-affinity peptide ligands for single GPCRs remains an unmet challenge. Here, we develop a computational approach for designing conjugates of lariat-shaped macrocyclized peptides and a small molecule opioid ligand. We demonstrate its feasibility by discovering chemical scaffolds for the kappa-opioid receptor (KOR) with desired pharmacological activities. The designed De Novo Cyclic Peptide (DNCP)-β-naloxamine (NalA) exhibit in vitro potent mixed KOR agonism/mu-opioid receptor (MOR) antagonism, nanomolar binding affinity, selectivity, and efficacy bias at KOR. Proof-of-concept in vivo efficacy studies demonstrate that DNCP-β-NalA(1) induces a potent KOR-mediated antinociception in male mice. The high-resolution cryo-EM structure (2.6 Å) of the DNCP-β-NalA–KOR–Gi1 complex and molecular dynamics simulations are harnessed to validate the computational design model. This reveals a network of residues in ECL2/3 and TM6/7 controlling the intrinsic efficacy of KOR. In general, our computational de novo platform overcomes extensive lead optimization encountered in ultra-large library docking and virtual small molecule screening campaigns and offers innovation for GPCR ligand discovery. This may drive the development of next-generation therapeutics for medical applications such as pain conditions. Despite advances in GPCR structures and peptide design, creating high-affinity ligands remains a challenge. Here the authors develop a computational method, successfully identifying peptide-based molecules for KOR: their platform shows promise for streamlined GPCR ligand discovery."/> <meta name="dc.creator" content="Muratspahić, Edin"/> <meta name="dc.creator" content="Deibler, Kristine"/> <meta name="dc.creator" content="Han, Jianming"/> <meta name="dc.creator" content="Tomašević, Nataša"/> <meta name="dc.creator" content="Jadhav, Kirtikumar B."/> <meta name="dc.creator" content="Olivé-Marti, Aina-Leonor"/> <meta name="dc.creator" content="Hochrainer, Nadine"/> <meta name="dc.creator" content="Hellinger, Roland"/> <meta name="dc.creator" content="Koehbach, Johannes"/> <meta name="dc.creator" content="Fay, Jonathan F."/> <meta name="dc.creator" content="Rahman, Mohammad Homaidur"/> <meta name="dc.creator" content="Hegazy, Lamees"/> <meta name="dc.creator" content="Craven, Timothy W."/> <meta name="dc.creator" content="Varga, Balazs R."/> <meta name="dc.creator" content="Bhardwaj, Gaurav"/> <meta name="dc.creator" content="Appourchaux, Kevin"/> <meta name="dc.creator" content="Majumdar, Susruta"/> <meta name="dc.creator" content="Muttenthaler, Markus"/> <meta name="dc.creator" content="Hosseinzadeh, Parisa"/> <meta name="dc.creator" content="Craik, David J."/> <meta name="dc.creator" content="Spetea, Mariana"/> <meta name="dc.creator" content="Che, Tao"/> <meta name="dc.creator" content="Baker, David"/> <meta name="dc.creator" content="Gruber, Christian W."/> <meta name="dc.subject" content="Peptides"/> <meta name="dc.subject" content="Protein design"/> <meta name="dc.subject" content="Receptor pharmacology"/> <meta name="citation_reference" content="citation_journal_title=Nat. Rev. Drug Discov.; citation_title=Advances in therapeutic peptides targeting G protein-coupled receptors; citation_author=AP Davenport, CCG Scully, C Graaf, AJH Brown, JJ Maguire; citation_volume=19; citation_publication_date=2020; citation_pages=389-413; citation_doi=10.1038/s41573-020-0062-z; citation_id=CR1"/> <meta name="citation_reference" content="citation_journal_title=Nat. Rev. Drug Discov.; citation_title=Trends in GPCR drug discovery: new agents, targets and indications; citation_author=AS Hauser, MM Attwood, M Rask-Andersen, HB Schioth, DE Gloriam; citation_volume=16; citation_publication_date=2017; citation_pages=829-842; citation_doi=10.1038/nrd.2017.178; citation_id=CR2"/> <meta name="citation_reference" content="citation_journal_title=Trends Pharmacol. Sci.; citation_title=Nature-derived peptides: a growing niche for GPCR ligand discovery; citation_author=E Muratspahic, M Freissmuth, CW Gruber; citation_volume=40; citation_publication_date=2019; citation_pages=309-326; citation_doi=10.1016/j.tips.2019.03.004; citation_id=CR3"/> <meta name="citation_reference" content="citation_journal_title=Nat. Rev. Drug Discov.; citation_title=Trends in peptide drug discovery; citation_author=M Muttenthaler, GF King, DJ Adams, PF Alewood; citation_volume=20; citation_publication_date=2021; citation_pages=309-325; citation_doi=10.1038/s41573-020-00135-8; citation_id=CR4"/> <meta name="citation_reference" content="citation_journal_title=N. Engl. J. Med.; citation_title=The role of science in addressing the opioid crisis; citation_author=ND Volkow, FS Collins; citation_volume=377; citation_publication_date=2017; citation_pages=391-394; citation_doi=10.1056/NEJMsr1706626; citation_id=CR5"/> <meta name="citation_reference" content="citation_journal_title=Nat. Rev. Neurosci.; citation_title=Opioid receptors: drivers to addiction?; citation_author=E Darcq, BL Kieffer; citation_volume=19; citation_publication_date=2018; citation_pages=499-514; citation_doi=10.1038/s41583-018-0028-x; citation_id=CR6"/> <meta name="citation_reference" content="citation_journal_title=ACS Chem. Neurosci.; citation_title=Novel opioid analgesics and side effects; citation_author=G Vecchio, V Spahn, C Stein; citation_volume=8; citation_publication_date=2017; citation_pages=1638-1640; citation_doi=10.1021/acschemneuro.7b00195; citation_id=CR7"/> <meta name="citation_reference" content="citation_journal_title=Cell; citation_title=Structure of the nanobody-stabilized active state of the kappa opioid receptor; citation_author=T Che; citation_volume=172; citation_publication_date=2018; citation_pages=55-67; citation_doi=10.1016/j.cell.2017.12.011; citation_id=CR8"/> <meta name="citation_reference" content="citation_journal_title=Sci. Signal.; citation_title=Biased ligands at opioid receptors: current status and future directions; citation_author=T Che, H Dwivedi-Agnihotri, AK Shukla, BL Roth; citation_volume=14; citation_publication_date=2021; citation_pages=aav0320; citation_doi=10.1126/scisignal.aav0320; citation_id=CR9"/> <meta name="citation_reference" content="citation_journal_title=Front. Neurosci.; citation_title=Signaling underlying kappa opioid receptor-mediated behaviors in rodents; citation_author=LY Liu-Chen, P Huang; citation_volume=16; citation_publication_date=2022; citation_pages=964724; citation_doi=10.3389/fnins.2022.964724; citation_id=CR10"/> <meta name="citation_reference" content="citation_journal_title=Front. Pharmacol.; citation_title=Advances in achieving opioid analgesia without side effects; citation_author=H Machelska, MO Celik; citation_volume=9; citation_publication_date=2018; citation_pages=1388; citation_doi=10.3389/fphar.2018.01388; citation_id=CR11"/> <meta name="citation_reference" content="citation_journal_title=Front. Pharmacol.; citation_title=The kappa opioid receptor: a promising therapeutic target for multiple pathologies; citation_author=ML Dalefield, B Scouller, R Bibi, BM Kivell; citation_volume=13; citation_publication_date=2022; citation_pages=837671; citation_doi=10.3389/fphar.2022.837671; citation_id=CR12"/> <meta name="citation_reference" content="Bachmutsky, I., Wei, X. P., Durand, A. & Yackle, K. β-arrestin 2 germline knockout does not attenuate opioid respiratory depression. Elife 10, https://doi.org/10.7554/eLife.62552 (2021)."/> <meta name="citation_reference" content="citation_journal_title=Nat. Commun.; citation_title=Phosphorylation-deficient G-protein-biased mu-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects; citation_author=A Kliewer; citation_volume=10; citation_publication_date=2019; citation_doi=10.1038/s41467-018-08162-1; citation_id=CR14"/> <meta name="citation_reference" content="citation_journal_title=ACS Chem. Neurosci.; citation_title=Discovery of small molecule kappa opioid receptor agonist and antagonist chemotypes through a HTS and hit refinement strategy; citation_author=KJ Frankowski; citation_volume=3; citation_publication_date=2012; citation_pages=221-236; citation_doi=10.1021/cn200128x; citation_id=CR15"/> <meta name="citation_reference" content="citation_journal_title=J. Chem. Inf. Model; citation_title=Discovery of a novel selective kappa-opioid receptor agonist using crystal structure-based virtual screening; citation_author=A Negri; citation_volume=53; citation_publication_date=2013; citation_pages=521-526; citation_doi=10.1021/ci400019t; citation_id=CR16"/> <meta name="citation_reference" content="citation_journal_title=Proc. Natl Acad. Sci. USA; citation_title=Salvinorin A: a potent naturally occurring nonnitrogenous kappa opioid selective agonist; citation_author=BL Roth; citation_volume=99; citation_publication_date=2002; citation_pages=11934-11939; citation_doi=10.1073/pnas.182234399; citation_id=CR17"/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_title=Accurate de novo design of hyperstable constrained peptides; citation_author=G Bhardwaj; citation_volume=538; citation_publication_date=2016; citation_pages=329-335; citation_doi=10.1038/nature19791; citation_id=CR18"/> <meta name="citation_reference" content="citation_journal_title=Science; citation_title=De novo design of picomolar SARS-CoV-2 miniprotein inhibitors; citation_author=L Cao; citation_volume=370; citation_publication_date=2020; citation_pages=426-431; citation_doi=10.1126/science.abd9909; citation_id=CR19"/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_title=Massively parallel de novo protein design for targeted therapeutics; citation_author=A Chevalier; citation_volume=550; citation_publication_date=2017; citation_pages=74-79; citation_doi=10.1038/nature23912; citation_id=CR20"/> <meta name="citation_reference" content="citation_journal_title=Science; citation_title=Comprehensive computational design of ordered peptide macrocycles; citation_author=P Hosseinzadeh; citation_volume=358; citation_publication_date=2017; citation_pages=1461-1466; citation_doi=10.1126/science.aap7577; citation_id=CR21"/> <meta name="citation_reference" content="citation_journal_title=Nat. Commun.; citation_title=Anchor extension: a structure-guided approach to design cyclic peptides targeting enzyme active sites; citation_author=P Hosseinzadeh; citation_volume=12; citation_publication_date=2021; citation_doi=10.1038/s41467-021-23609-8; citation_id=CR22"/> <meta name="citation_reference" content="citation_journal_title=Proc. Natl Acad. Sci. USA; citation_title=Computationally designed peptide macrocycle inhibitors of New Delhi metallo-beta-lactamase 1; citation_author=VK Mulligan; citation_volume=118; citation_publication_date=2021; citation_pages=e2012800118; citation_doi=10.1073/pnas.2012800118; citation_id=CR23"/> <meta name="citation_reference" content="citation_journal_title=Nat. Commun.; citation_title=Routine sub-2.5 Å cryo-EM structure determination of GPCRs; citation_author=R Danev; citation_volume=12; citation_publication_date=2021; citation_doi=10.1038/s41467-021-24650-3; citation_id=CR24"/> <meta name="citation_reference" content="citation_journal_title=Nat. Commun.; citation_title=The pocketome of G protein-coupled receptors reveals previously untargeted allosteric sites; citation_author=JB Hedderich; citation_volume=13; citation_publication_date=2022; citation_doi=10.1038/s41467-022-29609-6; citation_id=CR25"/> <meta name="citation_reference" content="citation_journal_title=ACS Chem. Neurosci.; citation_title=Synthesis and characterization of a dual kappa-delta opioid receptor agonist analgesic blocking cocaine reward behavior; citation_author=A Varadi; citation_volume=6; citation_publication_date=2015; citation_pages=1813-1824; citation_doi=10.1021/acschemneuro.5b00153; citation_id=CR26"/> <meta name="citation_reference" content="citation_journal_title=J. Med. Chem.; citation_title=Synthesis and evaluation of aryl-naloxamide opiate analgesics targeting truncated exon 11-associated mu opioid receptor (MOR-1.) splice variants; citation_author=S Majumdar; citation_volume=55; citation_publication_date=2012; citation_pages=6352-6362; citation_doi=10.1021/jm300305c; citation_id=CR27"/> <meta name="citation_reference" content="citation_journal_title=J. Phys. Chem. B; citation_title=Effects of cyclization on peptide backbone dynamics; citation_author=CK Wang, JE Swedberg, SE Northfield, DJ Craik; citation_volume=119; citation_publication_date=2015; citation_pages=15821-15830; citation_doi=10.1021/acs.jpcb.5b11085; citation_id=CR28"/> <meta name="citation_reference" content="Uprety, R. et al. Controlling opioid receptor functional selectivity by targeting distinct subpockets of the orthosteric site. Elife 10, https://doi.org/10.7554/eLife.56519 (2021)."/> <meta name="citation_reference" content="citation_journal_title=Br. J. Pharmacol.; citation_title=Efficacy and ligand bias at the mu-opioid receptor; citation_author=E Kelly; citation_volume=169; citation_publication_date=2013; citation_pages=1430-1446; citation_doi=10.1111/bph.12222; citation_id=CR30"/> <meta name="citation_reference" content="citation_journal_title=Sci. Signal.; citation_title=Biased agonists of the kappa opioid receptor suppress pain and itch without causing sedation or dysphoria; citation_author=TF Brust; citation_volume=9; citation_publication_date=2016; citation_pages=aai8441; citation_doi=10.1126/scisignal.aai8441; citation_id=CR31"/> <meta name="citation_reference" content="Faouzi, A., Varga, B. R. & Majumdar, S. Biased opioid ligands. Molecules 25, https://doi.org/10.3390/molecules25184257 (2020)."/> <meta name="citation_reference" content="citation_journal_title=ACS Med. Chem. Lett.; citation_title=Seeking (and Finding) biased ligands of the kappa opioid receptor; citation_author=LM Bohn, J Aube; citation_volume=8; citation_publication_date=2017; citation_pages=694-700; citation_doi=10.1021/acsmedchemlett.7b00224; citation_id=CR33"/> <meta name="citation_reference" content="citation_journal_title=Effects. J. Pharmacol. Exp. Ther.; citation_title=Strategies for developing kappa opioid receptor agonists for the treatment of pain with fewer side; citation_author=KF Paton, DV Atigari, S Kaska, T Prisinzano, BM Kivell; citation_volume=375; citation_publication_date=2020; citation_pages=332-348; citation_doi=10.1124/jpet.120.000134; citation_id=CR34"/> <meta name="citation_reference" content="citation_journal_title=Nat. Chem. Biol.; citation_title=TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome; citation_author=RHJ Olsen; citation_volume=16; citation_publication_date=2020; citation_pages=841-849; citation_doi=10.1038/s41589-020-0535-8; citation_id=CR35"/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_title=Structure of the micro-opioid receptor-Gi protein complex; citation_author=A Koehl; citation_volume=558; citation_publication_date=2018; citation_pages=547-552; citation_doi=10.1038/s41586-018-0219-7; citation_id=CR36"/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_title=Structure of the human kappa-opioid receptor in complex with JDTic; citation_author=H Wu; citation_volume=485; citation_publication_date=2012; citation_pages=327-332; citation_doi=10.1038/nature10939; citation_id=CR37"/> <meta name="citation_reference" content="citation_journal_title=Cell; citation_title=Structures of the entire human opioid receptor family; citation_author=Y Wang; citation_volume=186; citation_publication_date=2023; citation_pages=413-427,; citation_doi=10.1016/j.cell.2022.12.026; citation_id=CR38"/> <meta name="citation_reference" content="citation_journal_title=Science; citation_title=Structure-based discovery of nonopioid analgesics acting through the alpha(2A)-adrenergic receptor; citation_author=EA Fink; citation_volume=377; citation_publication_date=2022; citation_pages=eabn7065; citation_doi=10.1126/science.abn7065; citation_id=CR39"/> <meta name="citation_reference" content="citation_journal_title=Front. Pharmacol.; citation_title=Functional selectivity and antinociceptive effects of a novel KOPr agonist; citation_author=A Bedini; citation_volume=11; citation_publication_date=2020; citation_pages=188; citation_doi=10.3389/fphar.2020.00188; citation_id=CR40"/> <meta name="citation_reference" content="citation_journal_title=Br. J. Pharmacol.; citation_title=Selective kappa receptor partial agonist HS666 produces potent antinociception without inducing aversion after i.c.v. administration in mice; citation_author=M Spetea; citation_volume=174; citation_publication_date=2017; citation_pages=2444-2456; citation_doi=10.1111/bph.13854; citation_id=CR41"/> <meta name="citation_reference" content="Spetea, M. & Schmidhammer, H. Recent chemical and pharmacological developments on 14-oxygenated-N-methylmorphinan-6-ones. Molecules 26, https://doi.org/10.3390/molecules26185677 (2021)."/> <meta name="citation_reference" content="citation_journal_title=Nat. Chem. Biol.; citation_title=Structure-inspired design of beta-arrestin-biased ligands for aminergic GPCRs; citation_author=JD McCorvy; citation_volume=14; citation_publication_date=2018; citation_pages=126-134; citation_doi=10.1038/nchembio.2527; citation_id=CR43"/> <meta name="citation_reference" content="citation_journal_title=Front. Synaptic Neurosci.; citation_title=Structure-activity investigation of a G protein-biased agonist reveals molecular determinants for biased signaling of the D2 dopamine receptor; citation_author=LS Chun; citation_volume=10; citation_publication_date=2018; citation_pages=2; citation_doi=10.3389/fnsyn.2018.00002; citation_id=CR44"/> <meta name="citation_reference" content="citation_journal_title=Signal. Transduct. Target Ther.; citation_title=G protein-coupled receptors: structure- and function-based drug discovery; citation_author=D Yang; citation_volume=6; citation_publication_date=2021; citation_pages=7; citation_doi=10.1038/s41392-020-00435-w; citation_id=CR45"/> <meta name="citation_reference" content="citation_journal_title=J. Cheminform.; citation_title=Open Babel: an open chemical toolbox; citation_author=NM O’Boyle; citation_volume=3; citation_publication_date=2011; citation_pages=33; citation_doi=10.1186/1758-2946-3-33; citation_id=CR46"/> <meta name="citation_reference" content="citation_journal_title=J. Comput. Chem.; citation_title=A kinematic view of loop closure; citation_author=EA Coutsias, C Seok, MP Jacobson, KA Dill; citation_volume=25; citation_publication_date=2004; citation_pages=510-528; citation_doi=10.1002/jcc.10416; citation_id=CR47"/> <meta name="citation_reference" content="citation_journal_title=Nat. Methods; citation_title=Sub-angstrom accuracy in protein loop reconstruction by tics-inspired conformational sampling; citation_author=DJ Mandell, EA Coutsias, T Kortemme; citation_volume=6; citation_publication_date=2009; citation_pages=551-552; citation_doi=10.1038/nmeth0809-551; citation_id=CR48"/> <meta name="citation_reference" content="citation_journal_title=J. Mol. Biol.; citation_title=Alternate states of proteins revealed by detailed energy landscape mapping; citation_author=MD Tyka; citation_volume=405; citation_publication_date=2011; citation_pages=607-618; citation_doi=10.1016/j.jmb.2010.11.008; citation_id=CR49"/> <meta name="citation_reference" content="citation_journal_title=J. Org. Chem.; citation_title=Fmoc-based synthesis of disulfide-rich cyclic peptides; citation_author=O Cheneval; citation_volume=79; citation_publication_date=2014; citation_pages=5538-5544; citation_doi=10.1021/jo500699m; citation_id=CR50"/> <meta name="citation_reference" content="citation_journal_title=Proc. Natl Acad. Sci. USA; citation_title=Oxytocic plant cyclotides as templates for peptide G protein-coupled receptor ligand design; citation_author=J Koehbach; citation_volume=110; citation_publication_date=2013; citation_pages=21183-21188; citation_doi=10.1073/pnas.1311183110; citation_id=CR51"/> <meta name="citation_reference" content="Dumitrascuta, M. et al. Antinociceptive efficacy of the micro-opioid/nociceptin peptide-based Hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking. Molecules 26, https://doi.org/10.3390/molecules26113267 (2021)."/> <meta name="citation_reference" content="citation_journal_title=J. Med. Chem.; citation_title=Highly potent and selective new diphenethylamines interacting with the kappa-opioid receptor: synthesis, pharmacology, and structure-activity relationships; citation_author=F Erli; citation_volume=60; citation_publication_date=2017; citation_pages=7579-7590; citation_doi=10.1021/acs.jmedchem.7b00981; citation_id=CR53"/> <meta name="citation_reference" content="citation_journal_title=Anal. Biochem.; citation_title=A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding; citation_author=MM Bradford; citation_volume=72; citation_publication_date=1976; citation_pages=248-254; citation_doi=10.1016/0003-2697(76)90527-3; citation_id=CR54"/> <meta name="citation_reference" content="citation_journal_title=Sci. Rep.; citation_title=I8-arachnotocin-an arthropod-derived G protein-biased ligand of the human vasopressin V2 receptor; citation_author=L Duerrauer; citation_volume=9; citation_publication_date=2019; citation_doi=10.1038/s41598-019-55675-w; citation_id=CR55"/> <meta name="citation_reference" content="citation_journal_title=Mol. Pharmacol.; citation_title=Functional impact of the G279S substitution in the adenosine A(1)-Receptor (A(1)R-G279S(7.44)), a mutation associated with Parkinson’s disease; citation_author=S Nasrollahi-Shirazi; citation_volume=98; citation_publication_date=2020; citation_pages=250-266; citation_doi=10.1124/molpharm.120.000003; citation_id=CR56"/> <meta name="citation_reference" content="Nasrollahi-Shirazi, S. et al. Functional impact of the G279S substitution in the adenosine A1-Receptor (A1R-G279S(7.44)), a mutation associated with Parkinson’s disease. Mol. Pharmacol. 98, 250–266 (2020)."/> <meta name="citation_reference" content="citation_journal_title=J. Med. Chem.; citation_title=Design of a stable cyclic peptide analgesic derived from sunflower seeds that targets the kappa-opioid receptor for the treatment of chronic abdominal pain; citation_author=E Muratspahic; citation_volume=64; citation_publication_date=2021; citation_pages=9042-9055; citation_doi=10.1021/acs.jmedchem.1c00158; citation_id=CR58"/> <meta name="citation_reference" content="citation_journal_title=Nat. Commun.; citation_title=Development of an antibody fragment that stabilizes GPCR/G-protein complexes; citation_author=S Maeda; citation_volume=9; citation_publication_date=2018; citation_doi=10.1038/s41467-018-06002-w; citation_id=CR59"/> <meta name="citation_reference" content="citation_journal_title=ACS Pharmacol. Transl. Sci.; citation_title=Ominant negative G proteins enhance formation and purification of agonist-GPCR-G protein complexes for structure determination; citation_author=YL Liang; citation_volume=1; citation_publication_date=2018; citation_pages=12-20; citation_doi=10.1021/acsptsci.8b00017; citation_id=CR60"/> <meta name="citation_reference" content="citation_journal_title=Cell; citation_title=Structure of a hallucinogen-activated Gq-coupled 5-HT2A serotonin receptor; citation_author=K Kim; citation_volume=182; citation_publication_date=2020; citation_pages=1574-1588; citation_doi=10.1016/j.cell.2020.08.024; citation_id=CR61"/> <meta name="citation_reference" content="citation_journal_title=IUCrJ; citation_title=High-speed high-resolution data collection on a 200 keV cryo-TEM; citation_author=JV Peck, JF Fay, JD Strauss; citation_volume=9; citation_publication_date=2022; citation_pages=243-252; citation_doi=10.1107/S2052252522000069; citation_id=CR62"/> <meta name="citation_reference" content="citation_journal_title=Nat. Methods; citation_title=A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination; citation_author=A Punjani, JL Rubinstein, DJ Fleet, M Brubaker; citation_volume=14; citation_publication_date=2017; citation_pages=290-296; citation_doi=10.1038/nmeth.4169; citation_id=CR63"/> <meta name="citation_reference" content="citation_journal_title=Nat. Methods; citation_title=Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction; citation_author=A Punjani, H Zhang, DJ Fleet; citation_volume=17; citation_publication_date=2020; citation_pages=1214-1221; citation_doi=10.1038/s41592-020-00990-8; citation_id=CR64"/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_title=Structure, function and pharmacology of human itch GPCRs; citation_author=C Cao; citation_volume=600; citation_publication_date=2021; citation_pages=170-175; citation_doi=10.1038/s41586-021-04126-6; citation_id=CR65"/> <meta name="citation_reference" content="citation_journal_title=J. Comput. Chem.; citation_title=UCSF Chimera–a visualization system for exploratory research and analysis; citation_author=EF Pettersen; citation_volume=25; citation_publication_date=2004; citation_pages=1605-1612; citation_doi=10.1002/jcc.20084; citation_id=CR66"/> <meta name="citation_reference" content="citation_journal_title=Acta Crystallogr. D Biol. Crystallogr.; citation_title=Features and development of Coot; citation_author=P Emsley, B Lohkamp, WG Scott, K Cowtan; citation_volume=66; citation_publication_date=2010; citation_pages=486-501; citation_doi=10.1107/S0907444910007493; citation_id=CR67"/> <meta name="citation_reference" content="citation_journal_title=Acta Crystallogr. D Biol. Crystallogr.; citation_title=PHENIX: a comprehensive Python-based system for macromolecular structure solution; citation_author=PD Adams; citation_volume=66; citation_publication_date=2010; citation_pages=213-221; citation_doi=10.1107/S0907444909052925; citation_id=CR68"/> <meta name="citation_reference" content="citation_journal_title=Acta Crystallogr. D Biol. Crystallogr.; citation_title=MolProbity: all-atom structure validation for macromolecular crystallography; citation_author=VB Chen; citation_volume=66; citation_publication_date=2010; citation_pages=12-21; citation_doi=10.1107/S0907444909042073; citation_id=CR69"/> <meta name="citation_reference" content="Case, D. A. et al. Amber 2023 (University of California, 2023)."/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_title=Highly accurate protein structure prediction with AlphaFold; citation_author=J Jumper; citation_volume=596; citation_publication_date=2021; citation_pages=583-589; citation_doi=10.1038/s41586-021-03819-2; citation_id=CR71"/> <meta name="citation_reference" content="citation_journal_title=J. Chem. Inf. Model; citation_title=PACKMOL-Memgen: a simple-to-use, generalized workflow for membrane-protein-lipid-bilayer system building; citation_author=S Schott-Verdugo, H Gohlke; citation_volume=59; citation_publication_date=2019; citation_pages=2522-2528; citation_doi=10.1021/acs.jcim.9b00269; citation_id=CR72"/> <meta name="citation_reference" content="citation_journal_title=Nature; citation_title=Ligand and G-protein selectivity in the kappa-opioid receptor; citation_author=J Han; citation_volume=617; citation_publication_date=2023; citation_pages=417-425; citation_doi=10.1038/s41586-023-06030-7; citation_id=CR73"/> <meta name="citation_reference" content="citation_journal_title=J. Chem. Theory Comput.; citation_title=ff19SB: amino-acid-specific protein backbone parameters trained against quantum mechanics energy surfaces in solution; citation_author=C Tian; citation_volume=16; citation_publication_date=2020; citation_pages=528-552; citation_doi=10.1021/acs.jctc.9b00591; citation_id=CR74"/> <meta name="citation_reference" content="citation_journal_title=J. Chem. Theory Comput.; citation_title=Lipid21: complex lipid membrane simulations with AMBER; citation_author=CJ Dickson, RC Walker, IR Gould; citation_volume=18; citation_publication_date=2022; citation_pages=1726-1736; citation_doi=10.1021/acs.jctc.1c01217; citation_id=CR75"/> <meta name="citation_reference" content="citation_journal_title=J. Comput. Chem; citation_title=Development and testing of a general amber force field; citation_author=J Wang, RM Wolf, JW Caldwell, PA Kollman, DA Case; citation_volume=25; citation_publication_date=2004; citation_pages=1157-1174; citation_doi=10.1002/jcc.20035; citation_id=CR76"/> <meta name="citation_reference" content="citation_journal_title=J. Chem. Phys.; citation_title=Comparison of simple potential functions for simulating liquid water; citation_author=WL Jorgensen, J Chandrasekhar, JD Madura, RW Impey, ML Klein; citation_volume=79; citation_publication_date=1983; citation_pages=926-935; citation_doi=10.1063/1.445869; citation_id=CR77"/> <meta name="citation_reference" content="citation_journal_title=J. Phys. Chem. B; citation_title=Determination of alkali and halide monovalent ion parameters for use in explicitly solvated biomolecular simulations; citation_author=IS Joung, TE Cheatham; citation_volume=112; citation_publication_date=2008; citation_pages=9020-9041; citation_doi=10.1021/jp8001614; citation_id=CR78"/> <meta name="citation_reference" content="citation_journal_title=Proc. Natl Acad. Sci. USA; citation_title=Native contacts determine protein folding mechanisms in atomistic simulations; citation_author=RB Best, G Hummer, WA Eaton; citation_volume=110; citation_publication_date=2013; citation_pages=17874-17879; citation_doi=10.1073/pnas.1311599110; citation_id=CR79"/> <meta name="citation_reference" content="citation_journal_title=J.Mol. Graph.; citation_title=VMD: visual molecular dynamics; citation_author=W Humphrey, A Dalke, K Schulten; citation_volume=14; citation_publication_date=1996; citation_pages=27-38; citation_doi=10.1016/0263-7855(96)00018-5; citation_id=CR80"/> <meta name="citation_reference" content="citation_journal_title=Pain; citation_title=The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats; citation_author=D Dubuisson, SG Dennis; citation_volume=4; citation_publication_date=1977; citation_pages=161-174; citation_doi=10.1016/0304-3959(77)90130-0; citation_id=CR81"/> <meta name="citation_reference" content="Puls, K. et al. In vitro, In vivo and In silico Characterization of a Novel Kappa-opioid receptor antagonist. Pharmaceuticals 15, https://doi.org/10.3390/ph15060680 (2022)."/> <meta name="citation_reference" content="citation_journal_title=J. Pharmacol. Exp. Ther.; citation_title=A simplified method of evaluating dose-effect experiments; citation_author=JT Litchfield, F Wilcoxon; citation_volume=96; citation_publication_date=1949; citation_pages=99-113; citation_id=CR83"/> <meta name="citation_reference" content="citation_journal_title=Pain; citation_title=A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia; citation_author=K Hargreaves, R Dubner, F Brown, C Flores, J Joris; citation_volume=32; citation_publication_date=1988; citation_pages=77-88; citation_doi=10.1016/0304-3959(88)90026-7; citation_id=CR84"/> <meta name="citation_reference" content="citation_journal_title=J. Pharm. Pharmacol; citation_title=The quantiative measurement of motor inco-ordination in naive mice using an acelerating rotarod; citation_author=BJ Jones, DJ Roberts; citation_volume=20; citation_publication_date=1968; citation_pages=302-304; citation_doi=10.1111/j.2042-7158.1968.tb09743.x; citation_id=CR85"/> <meta name="citation_reference" content="citation_journal_title=Biochem. Pharmacol.; citation_title=Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction; citation_author=Y Cheng, WH Prusoff; citation_volume=22; citation_publication_date=1973; citation_pages=3099-3108; citation_doi=10.1016/0006-2952(73)90196-2; citation_id=CR86"/> <meta name="citation_author" content="Muratspahić, Edin"/> <meta name="citation_author_institution" content="Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria"/> <meta name="citation_author_institution" content="Institute for Protein Design, University of Washington, Seattle, USA"/> <meta name="citation_author" content="Deibler, Kristine"/> <meta name="citation_author_institution" content="Institute for Protein Design, University of Washington, Seattle, USA"/> <meta name="citation_author_institution" content="Novo Nordisk Research Center Seattle, Novo Nordisk A/S, Seattle, USA"/> <meta name="citation_author" content="Han, Jianming"/> <meta name="citation_author_institution" content="Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author" content="Tomašević, Nataša"/> <meta name="citation_author_institution" content="Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria"/> <meta name="citation_author" content="Jadhav, Kirtikumar B."/> <meta name="citation_author_institution" content="Institute of Biological Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria"/> <meta name="citation_author" content="Olivé-Marti, Aina-Leonor"/> <meta name="citation_author_institution" content="Department of Pharmaceutical Chemistry, Institute of Pharmacy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria"/> <meta name="citation_author" content="Hochrainer, Nadine"/> <meta name="citation_author_institution" content="Department of Pharmaceutical Chemistry, Institute of Pharmacy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria"/> <meta name="citation_author" content="Hellinger, Roland"/> <meta name="citation_author_institution" content="Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria"/> <meta name="citation_author" content="Koehbach, Johannes"/> <meta name="citation_author_institution" content="Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Australia"/> <meta name="citation_author_institution" content="School of Biomedical Sciences, Faculty for Medicine, The University of Queensland, Brisbane, Australia"/> <meta name="citation_author" content="Fay, Jonathan F."/> <meta name="citation_author_institution" content="Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, USA"/> <meta name="citation_author" content="Rahman, Mohammad Homaidur"/> <meta name="citation_author_institution" content="Department of Pharmaceutical and Administrative Sciences, Saint Louis College of Pharmacy, University of Health Sciences & Pharmacy in St. Louis, St. Louis, USA"/> <meta name="citation_author" content="Hegazy, Lamees"/> <meta name="citation_author_institution" content="Department of Pharmaceutical and Administrative Sciences, Saint Louis College of Pharmacy, University of Health Sciences & Pharmacy in St. Louis, St. Louis, USA"/> <meta name="citation_author" content="Craven, Timothy W."/> <meta name="citation_author_institution" content="Institute for Protein Design, University of Washington, Seattle, USA"/> <meta name="citation_author" content="Varga, Balazs R."/> <meta name="citation_author_institution" content="Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author_institution" content="Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author" content="Bhardwaj, Gaurav"/> <meta name="citation_author_institution" content="Institute for Protein Design, University of Washington, Seattle, USA"/> <meta name="citation_author" content="Appourchaux, Kevin"/> <meta name="citation_author_institution" content="Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author_institution" content="Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author" content="Majumdar, Susruta"/> <meta name="citation_author_institution" content="Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author_institution" content="Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author" content="Muttenthaler, Markus"/> <meta name="citation_author_institution" content="Institute of Biological Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria"/> <meta name="citation_author_institution" content="Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia"/> <meta name="citation_author" content="Hosseinzadeh, Parisa"/> <meta name="citation_author_institution" content="Department of Bioengineering, Knight Campus, University of Oregon, Eugene, USA"/> <meta name="citation_author" content="Craik, David J."/> <meta name="citation_author_institution" content="Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Australia"/> <meta name="citation_author" content="Spetea, Mariana"/> <meta name="citation_author_institution" content="Department of Pharmaceutical Chemistry, Institute of Pharmacy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria"/> <meta name="citation_author" content="Che, Tao"/> <meta name="citation_author_institution" content="Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author_institution" content="Department of Anesthesiology, Washington University School of Medicine, St. Louis, USA"/> <meta name="citation_author" content="Baker, David"/> <meta name="citation_author_institution" content="Institute for Protein Design, University of Washington, Seattle, USA"/> <meta name="citation_author_institution" content="Department of Biochemistry, University of Washington, Seattle, USA"/> <meta name="citation_author_institution" content="Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA"/> <meta name="citation_author" content="Gruber, Christian W."/> <meta name="citation_author_institution" content="Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, Vienna, Austria"/> <meta name="access_endpoint" content="https://www.nature.com/platform/readcube-access"/> <meta name="twitter:site" content="@NatureComms"/> <meta name="twitter:card" content="summary_large_image"/> <meta name="twitter:image:alt" content="Content cover image"/> <meta name="twitter:title" content="Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor"/> <meta name="twitter:description" content="Nature Communications - Despite advances in GPCR structures and peptide design, creating high-affinity ligands remains a challenge. Here the authors develop a computational method, successfully..."/> <meta name="twitter:image" content="https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig1_HTML.png"/> <meta property="og:url" content="https://www.nature.com/articles/s41467-023-43718-w"/> <meta property="og:type" content="article"/> <meta property="og:site_name" content="Nature"/> <meta property="og:title" content="Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor - Nature Communications"/> <meta property="og:description" content="Despite advances in GPCR structures and peptide design, creating high-affinity ligands remains a challenge. Here the authors develop a computational method, successfully identifying peptide-based molecules for KOR: their platform shows promise for streamlined GPCR ligand discovery."/> <meta property="og:image" content="https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig1_HTML.png"/> <script> window.eligibleForRa21 = 'false'; </script> </head> <body class="article-page"> <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-MRVXSHQ" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript> <div class="position-relative cleared z-index-50 background-white" data-test="top-containers"> <a class="c-skip-link" href="#content">Skip to main content</a> <div class="c-grade-c-banner u-hide"> <div class="c-grade-c-banner__container"> <p>Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.</p> </div> </div> <div class="u-hide u-show-following-ad"></div> <aside class="c-ad c-ad--728x90"> <div class="c-ad__inner" data-container-type="banner-advert"> <p class="c-ad__label">Advertisement</p> <div id="div-gpt-ad-top-1" class="div-gpt-ad advert leaderboard js-ad text-center hide-print grade-c-hide" data-ad-type="top" data-test="top-ad" data-pa11y-ignore data-gpt data-gpt-unitpath="/285/nature_communications/article" data-gpt-sizes="728x90" data-gpt-targeting="type=article;pos=top;artid=s41467-023-43718-w;doi=10.1038/s41467-023-43718-w;techmeta=101,118,119,28,49,60,64,75,95,96;subjmeta=114,154,2387,436,469,611,631,92;kwrd=Peptides,Protein+design,Receptor+pharmacology"> <noscript> <a href="//pubads.g.doubleclick.net/gampad/jump?iu=/285/nature_communications/article&sz=728x90&c=240245840&t=pos%3Dtop%26type%3Darticle%26artid%3Ds41467-023-43718-w%26doi%3D10.1038/s41467-023-43718-w%26techmeta%3D101,118,119,28,49,60,64,75,95,96%26subjmeta%3D114,154,2387,436,469,611,631,92%26kwrd%3DPeptides,Protein+design,Receptor+pharmacology"> <img data-test="gpt-advert-fallback-img" src="//pubads.g.doubleclick.net/gampad/ad?iu=/285/nature_communications/article&sz=728x90&c=240245840&t=pos%3Dtop%26type%3Darticle%26artid%3Ds41467-023-43718-w%26doi%3D10.1038/s41467-023-43718-w%26techmeta%3D101,118,119,28,49,60,64,75,95,96%26subjmeta%3D114,154,2387,436,469,611,631,92%26kwrd%3DPeptides,Protein+design,Receptor+pharmacology" alt="Advertisement" width="728" height="90"></a> </noscript> </div> </div> </aside> <header class="c-header" id="header" data-header data-track-component="nature-150-split-header" style="border-color:#e63323"> <div class="c-header__row"> <div class="c-header__container"> <div class="c-header__split"> <div class="c-header__logo-container"> <a href="/ncomms" data-track="click" data-track-action="home" data-track-label="image"> <picture class="c-header__logo"> <source srcset="https://media.springernature.com/full/nature-cms/uploads/product/ncomms/header-03d2e325c0a02f6df509e5730e9be304.svg" media="(min-width: 875px)"> <img src="https://media.springernature.com/full/nature-cms/uploads/product/ncomms/header-7001f06bc3fe2437048388e9f2f44215.svg" height="32" alt="Nature Communications"> </picture> </a> </div> <ul class="c-header__menu c-header__menu--global"> <li class="c-header__item c-header__item--padding c-header__item--hide-md-max"> <a class="c-header__link" href="https://www.nature.com/siteindex" data-test="siteindex-link" data-track="click" data-track-action="open nature research index" data-track-label="link"> <span>View all journals</span> </a> </li> <li class="c-header__item c-header__item--padding c-header__item--pipe"> <a class="c-header__link c-header__link--search" href="#search-menu" data-header-expander data-test="search-link" data-track="click" data-track-action="open search tray" data-track-label="button"> <svg role="img" aria-hidden="true" focusable="false" height="22" width="22" viewBox="0 0 18 18" xmlns="http://www.w3.org/2000/svg"><path d="M16.48 15.455c.283.282.29.749.007 1.032a.738.738 0 01-1.032-.007l-3.045-3.044a7 7 0 111.026-1.026zM8 14A6 6 0 108 2a6 6 0 000 12z"/></svg><span>Search</span> </a> </li> <li class="c-header__item c-header__item--padding c-header__item--snid-account-widget c-header__item--pipe"> <a class="c-header__link eds-c-header__link" id="identity-account-widget" href='https://idp.nature.com/auth/personal/springernature?redirect_uri=https://www.nature.com/articles/s41467-023-43718-w?error=cookies_not_supported&code=1be584cf-d3be-4749-87ed-52b5fedc5509'><span class="eds-c-header__widget-fragment-title">Log in</span></a> </li> </ul> </div> </div> </div> <div class="c-header__row"> <div class="c-header__container" data-test="navigation-row"> <div class="c-header__split"> <ul class="c-header__menu c-header__menu--journal"> <li class="c-header__item c-header__item--dropdown-menu" data-test="explore-content-button"> <a href="#explore" class="c-header__link" data-header-expander data-test="menu-button--explore" data-track="click" data-track-action="open explore expander" data-track-label="button"> <span><span class="c-header__show-text">Explore</span> content</span><svg role="img" aria-hidden="true" focusable="false" height="16" viewBox="0 0 16 16" width="16" xmlns="http://www.w3.org/2000/svg"><path d="m5.58578644 3-3.29289322-3.29289322c-.39052429-.39052429-.39052429-1.02368927 0-1.41421356s1.02368927-.39052429 1.41421356 0l4 4c.39052429.39052429.39052429 1.02368927 0 1.41421356l-4 4c-.39052429.39052429-1.02368927.39052429-1.41421356 0s-.39052429-1.02368927 0-1.41421356z" transform="matrix(0 1 -1 0 11 3)"/></svg> </a> </li> <li class="c-header__item c-header__item--dropdown-menu"> <a href="#about-the-journal" class="c-header__link" data-header-expander data-test="menu-button--about-the-journal" data-track="click" data-track-action="open about the journal expander" data-track-label="button"> <span>About <span class="c-header__show-text">the journal</span></span><svg role="img" aria-hidden="true" focusable="false" height="16" viewBox="0 0 16 16" width="16" xmlns="http://www.w3.org/2000/svg"><path d="m5.58578644 3-3.29289322-3.29289322c-.39052429-.39052429-.39052429-1.02368927 0-1.41421356s1.02368927-.39052429 1.41421356 0l4 4c.39052429.39052429.39052429 1.02368927 0 1.41421356l-4 4c-.39052429.39052429-1.02368927.39052429-1.41421356 0s-.39052429-1.02368927 0-1.41421356z" transform="matrix(0 1 -1 0 11 3)"/></svg> </a> </li> <li class="c-header__item c-header__item--dropdown-menu" data-test="publish-with-us-button"> <a href="#publish-with-us" class="c-header__link c-header__link--dropdown-menu" data-header-expander data-test="menu-button--publish" data-track="click" data-track-action="open publish with us expander" data-track-label="button"> <span>Publish <span class="c-header__show-text">with us</span></span><svg role="img" aria-hidden="true" focusable="false" height="16" viewBox="0 0 16 16" width="16" xmlns="http://www.w3.org/2000/svg"><path d="m5.58578644 3-3.29289322-3.29289322c-.39052429-.39052429-.39052429-1.02368927 0-1.41421356s1.02368927-.39052429 1.41421356 0l4 4c.39052429.39052429.39052429 1.02368927 0 1.41421356l-4 4c-.39052429.39052429-1.02368927.39052429-1.41421356 0s-.39052429-1.02368927 0-1.41421356z" transform="matrix(0 1 -1 0 11 3)"/></svg> </a> </li> </ul> <ul class="c-header__menu c-header__menu--hide-lg-max"> <li class="c-header__item"> <a class="c-header__link" href="https://idp.nature.com/auth/personal/springernature?redirect_uri=https%3A%2F%2Fwww.nature.com%2Fmy-account%2Falerts%2Fsubscribe-journal%3Flist-id%3D264%26journal-link%3Dhttps%253A%252F%252Fwww.nature.com%252Fncomms%252F" rel="nofollow" data-track="click" data-track-action="Sign up for alerts" data-track-label="link (desktop site header)" data-track-external> <span>Sign up for alerts</span><svg role="img" aria-hidden="true" focusable="false" height="18" viewBox="0 0 18 18" width="18" xmlns="http://www.w3.org/2000/svg"><path d="m4 10h2.5c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-3.08578644l-1.12132034 1.1213203c-.18753638.1875364-.29289322.4418903-.29289322.7071068v.1715729h14v-.1715729c0-.2652165-.1053568-.5195704-.2928932-.7071068l-1.7071068-1.7071067v-3.4142136c0-2.76142375-2.2385763-5-5-5-2.76142375 0-5 2.23857625-5 5zm3 4c0 1.1045695.8954305 2 2 2s2-.8954305 2-2zm-5 0c-.55228475 0-1-.4477153-1-1v-.1715729c0-.530433.21071368-1.0391408.58578644-1.4142135l1.41421356-1.4142136v-3c0-3.3137085 2.6862915-6 6-6s6 2.6862915 6 6v3l1.4142136 1.4142136c.3750727.3750727.5857864.8837805.5857864 1.4142135v.1715729c0 .5522847-.4477153 1-1 1h-4c0 1.6568542-1.3431458 3-3 3-1.65685425 0-3-1.3431458-3-3z" fill="#222"/></svg> </a> </li> <li class="c-header__item c-header__item--pipe"> <a class="c-header__link" href="https://www.nature.com/ncomms.rss" data-track="click" data-track-action="rss feed" data-track-label="link"> <span>RSS feed</span> </a> </li> </ul> </div> </div> </div> </header> <nav class="u-mb-16" aria-label="breadcrumbs"> <div class="u-container"> <ol class="c-breadcrumbs" itemscope itemtype="https://schema.org/BreadcrumbList"> <li class="c-breadcrumbs__item" id="breadcrumb0" itemprop="itemListElement" itemscope itemtype="https://schema.org/ListItem"><a class="c-breadcrumbs__link" href="/" itemprop="item" data-track="click" data-track-action="breadcrumb" data-track-category="header" data-track-label="link:nature"><span itemprop="name">nature</span></a><meta itemprop="position" content="1"> <svg class="c-breadcrumbs__chevron" role="img" aria-hidden="true" focusable="false" height="10" viewBox="0 0 10 10" width="10" xmlns="http://www.w3.org/2000/svg"> <path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill="#666" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/> </svg> </li><li class="c-breadcrumbs__item" id="breadcrumb1" itemprop="itemListElement" itemscope itemtype="https://schema.org/ListItem"><a class="c-breadcrumbs__link" href="/ncomms" itemprop="item" data-track="click" data-track-action="breadcrumb" data-track-category="header" data-track-label="link:nature communications"><span itemprop="name">nature communications</span></a><meta itemprop="position" content="2"> <svg class="c-breadcrumbs__chevron" role="img" aria-hidden="true" focusable="false" height="10" viewBox="0 0 10 10" width="10" xmlns="http://www.w3.org/2000/svg"> <path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill="#666" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/> </svg> </li><li class="c-breadcrumbs__item" id="breadcrumb2" itemprop="itemListElement" itemscope itemtype="https://schema.org/ListItem"><a class="c-breadcrumbs__link" href="/ncomms/articles?type=article" itemprop="item" data-track="click" data-track-action="breadcrumb" data-track-category="header" data-track-label="link:articles"><span itemprop="name">articles</span></a><meta itemprop="position" content="3"> <svg class="c-breadcrumbs__chevron" role="img" aria-hidden="true" focusable="false" height="10" viewBox="0 0 10 10" width="10" xmlns="http://www.w3.org/2000/svg"> <path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill="#666" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/> </svg> </li><li class="c-breadcrumbs__item" id="breadcrumb3" itemprop="itemListElement" itemscope itemtype="https://schema.org/ListItem"> <span itemprop="name">article</span><meta itemprop="position" content="4"></li> </ol> </div> </nav> </div> <div class="u-container u-mt-32 u-mb-32 u-clearfix" id="content" data-component="article-container" data-container-type="article"> <main class="c-article-main-column u-float-left js-main-column" data-track-component="article body"> <div class="c-context-bar u-hide" data-test="context-bar" data-context-bar aria-hidden="true"> <div class="c-context-bar__container u-container" data-track-context="sticky banner"> <div class="c-context-bar__title"> Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor </div> <div class="c-pdf-download u-clear-both js-pdf-download"> <a href="/articles/s41467-023-43718-w.pdf" class="u-button u-button--full-width u-button--primary u-justify-content-space-between c-pdf-download__link" data-article-pdf="true" data-readcube-pdf-url="true" data-test="download-pdf" data-draft-ignore="true" data-track="content_download" data-track-type="article pdf download" data-track-action="download pdf" data-track-label="link" data-track-external download> <span class="c-pdf-download__text">Download PDF</span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"><use xlink:href="#icon-download"/></svg> </a> </div> </div> </div> <article lang="en"> <div class="c-pdf-button__container u-mb-16 u-hide-at-lg js-context-bar-sticky-point-mobile"> <div class="c-pdf-container" data-track-context="article body"> <div class="c-pdf-download u-clear-both js-pdf-download"> <a href="/articles/s41467-023-43718-w.pdf" class="u-button u-button--full-width u-button--primary u-justify-content-space-between c-pdf-download__link" data-article-pdf="true" data-readcube-pdf-url="true" data-test="download-pdf" data-draft-ignore="true" data-track="content_download" data-track-type="article pdf download" data-track-action="download pdf" data-track-label="link" data-track-external download> <span class="c-pdf-download__text">Download PDF</span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"><use xlink:href="#icon-download"/></svg> </a> </div> </div> </div> <div class="c-article-header"> <header> <ul class="c-article-identifiers" data-test="article-identifier"> <li class="c-article-identifiers__item" data-test="article-category">Article</li> <li class="c-article-identifiers__item"> <a href="https://www.springernature.com/gp/open-research/about/the-fundamentals-of-open-access-and-open-research" data-track="click" data-track-action="open access" data-track-label="link" class="u-color-open-access" data-test="open-access">Open access</a> </li> <li class="c-article-identifiers__item">Published: <time datetime="2023-12-06">06 December 2023</time></li> </ul> <h1 class="c-article-title" data-test="article-title" data-article-title="">Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor</h1> <ul class="c-article-author-list c-article-author-list--short" data-test="authors-list" data-component-authors-activator="authors-list"><li class="c-article-author-list__item"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Edin-Muratspahi_-Aff1-Aff14" data-author-popup="auth-Edin-Muratspahi_-Aff1-Aff14" data-author-search="Muratspahić, Edin">Edin Muratspahić</a><sup class="u-js-hide"><a href="#Aff1">1</a></sup><sup class="u-js-hide"> <a href="#na1">na1</a></sup><sup class="u-js-hide"> <a href="#nAff14">nAff14</a></sup>, </li><li class="c-article-author-list__item"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Kristine-Deibler-Aff2-Aff15" data-author-popup="auth-Kristine-Deibler-Aff2-Aff15" data-author-search="Deibler, Kristine">Kristine Deibler</a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0002-2376-8456"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0002-2376-8456</a></span><sup class="u-js-hide"><a href="#Aff2">2</a></sup><sup class="u-js-hide"> <a href="#na1">na1</a></sup><sup class="u-js-hide"> <a href="#nAff15">nAff15</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Jianming-Han-Aff3" data-author-popup="auth-Jianming-Han-Aff3" data-author-search="Han, Jianming">Jianming Han</a><sup class="u-js-hide"><a href="#Aff3">3</a></sup><sup class="u-js-hide"> <a href="#na1">na1</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Nata_a-Toma_evi_-Aff1" data-author-popup="auth-Nata_a-Toma_evi_-Aff1" data-author-search="Tomašević, Nataša">Nataša Tomašević</a><sup class="u-js-hide"><a href="#Aff1">1</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Kirtikumar_B_-Jadhav-Aff4" data-author-popup="auth-Kirtikumar_B_-Jadhav-Aff4" data-author-search="Jadhav, Kirtikumar B.">Kirtikumar B. Jadhav</a><sup class="u-js-hide"><a href="#Aff4">4</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Aina_Leonor-Oliv__Marti-Aff5" data-author-popup="auth-Aina_Leonor-Oliv__Marti-Aff5" data-author-search="Olivé-Marti, Aina-Leonor">Aina-Leonor Olivé-Marti</a><sup class="u-js-hide"><a href="#Aff5">5</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Nadine-Hochrainer-Aff5" data-author-popup="auth-Nadine-Hochrainer-Aff5" data-author-search="Hochrainer, Nadine">Nadine Hochrainer</a><sup class="u-js-hide"><a href="#Aff5">5</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Roland-Hellinger-Aff1" data-author-popup="auth-Roland-Hellinger-Aff1" data-author-search="Hellinger, Roland">Roland Hellinger</a><sup class="u-js-hide"><a href="#Aff1">1</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Johannes-Koehbach-Aff6-Aff16" data-author-popup="auth-Johannes-Koehbach-Aff6-Aff16" data-author-search="Koehbach, Johannes">Johannes Koehbach</a><sup class="u-js-hide"><a href="#Aff6">6</a></sup><sup class="u-js-hide"> <a href="#nAff16">nAff16</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Jonathan_F_-Fay-Aff7" data-author-popup="auth-Jonathan_F_-Fay-Aff7" data-author-search="Fay, Jonathan F.">Jonathan F. Fay</a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0003-1822-2384"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0003-1822-2384</a></span><sup class="u-js-hide"><a href="#Aff7">7</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Mohammad_Homaidur-Rahman-Aff8" data-author-popup="auth-Mohammad_Homaidur-Rahman-Aff8" data-author-search="Rahman, Mohammad Homaidur">Mohammad Homaidur Rahman</a><sup class="u-js-hide"><a href="#Aff8">8</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Lamees-Hegazy-Aff8" data-author-popup="auth-Lamees-Hegazy-Aff8" data-author-search="Hegazy, Lamees">Lamees Hegazy</a><sup class="u-js-hide"><a href="#Aff8">8</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Timothy_W_-Craven-Aff2" data-author-popup="auth-Timothy_W_-Craven-Aff2" data-author-search="Craven, Timothy W.">Timothy W. Craven</a><sup class="u-js-hide"><a href="#Aff2">2</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Balazs_R_-Varga-Aff3-Aff9" data-author-popup="auth-Balazs_R_-Varga-Aff3-Aff9" data-author-search="Varga, Balazs R.">Balazs R. Varga</a><sup class="u-js-hide"><a href="#Aff3">3</a>,<a href="#Aff9">9</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Gaurav-Bhardwaj-Aff2" data-author-popup="auth-Gaurav-Bhardwaj-Aff2" data-author-search="Bhardwaj, Gaurav">Gaurav Bhardwaj</a><sup class="u-js-hide"><a href="#Aff2">2</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Kevin-Appourchaux-Aff3-Aff9" data-author-popup="auth-Kevin-Appourchaux-Aff3-Aff9" data-author-search="Appourchaux, Kevin">Kevin Appourchaux</a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0001-9853-3347"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0001-9853-3347</a></span><sup class="u-js-hide"><a href="#Aff3">3</a>,<a href="#Aff9">9</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Susruta-Majumdar-Aff3-Aff9" data-author-popup="auth-Susruta-Majumdar-Aff3-Aff9" data-author-search="Majumdar, Susruta">Susruta Majumdar</a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0002-2931-3823"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0002-2931-3823</a></span><sup class="u-js-hide"><a href="#Aff3">3</a>,<a href="#Aff9">9</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Markus-Muttenthaler-Aff4-Aff10" data-author-popup="auth-Markus-Muttenthaler-Aff4-Aff10" data-author-search="Muttenthaler, Markus">Markus Muttenthaler</a><sup class="u-js-hide"><a href="#Aff4">4</a>,<a href="#Aff10">10</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Parisa-Hosseinzadeh-Aff11" data-author-popup="auth-Parisa-Hosseinzadeh-Aff11" data-author-search="Hosseinzadeh, Parisa">Parisa Hosseinzadeh</a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0002-3128-7433"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0002-3128-7433</a></span><sup class="u-js-hide"><a href="#Aff11">11</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-David_J_-Craik-Aff6" data-author-popup="auth-David_J_-Craik-Aff6" data-author-search="Craik, David J.">David J. Craik</a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0003-0007-6796"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0003-0007-6796</a></span><sup class="u-js-hide"><a href="#Aff6">6</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Mariana-Spetea-Aff5" data-author-popup="auth-Mariana-Spetea-Aff5" data-author-search="Spetea, Mariana">Mariana Spetea</a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0002-2379-5358"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0002-2379-5358</a></span><sup class="u-js-hide"><a href="#Aff5">5</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Tao-Che-Aff3-Aff9" data-author-popup="auth-Tao-Che-Aff3-Aff9" data-author-search="Che, Tao" data-corresp-id="c1">Tao Che<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-mail-medium"></use></svg></a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0002-1620-3027"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0002-1620-3027</a></span><sup class="u-js-hide"><a href="#Aff3">3</a>,<a href="#Aff9">9</a></sup>, </li><li class="c-article-author-list__item c-article-author-list__item--hide-small-screen"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-David-Baker-Aff2-Aff12-Aff13" data-author-popup="auth-David-Baker-Aff2-Aff12-Aff13" data-author-search="Baker, David" data-corresp-id="c2">David Baker<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-mail-medium"></use></svg></a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0001-7896-6217"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0001-7896-6217</a></span><sup class="u-js-hide"><a href="#Aff2">2</a>,<a href="#Aff12">12</a>,<a href="#Aff13">13</a></sup> & </li><li class="c-article-author-list__show-more" aria-label="Show all 24 authors for this article" title="Show all 24 authors for this article">…</li><li class="c-article-author-list__item"><a data-test="author-name" data-track="click" data-track-action="open author" data-track-label="link" href="#auth-Christian_W_-Gruber-Aff1" data-author-popup="auth-Christian_W_-Gruber-Aff1" data-author-search="Gruber, Christian W." data-corresp-id="c3">Christian W. Gruber<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-mail-medium"></use></svg></a><span class="u-js-hide"> <a class="js-orcid" href="http://orcid.org/0000-0001-6060-7048"><span class="u-visually-hidden">ORCID: </span>orcid.org/0000-0001-6060-7048</a></span><sup class="u-js-hide"><a href="#Aff1">1</a></sup> </li></ul><button aria-expanded="false" class="c-article-author-list__button"><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-down-medium"></use></svg><span>Show authors</span></button> <p class="c-article-info-details" data-container-section="info"> <a data-test="journal-link" href="/ncomms" data-track="click" data-track-action="journal homepage" data-track-category="article body" data-track-label="link"><i data-test="journal-title">Nature Communications</i></a> <b data-test="journal-volume"><span class="u-visually-hidden">volume</span> 14</b>, Article number: <span data-test="article-number">8064</span> (<span data-test="article-publication-year">2023</span>) <a href="#citeas" class="c-article-info-details__cite-as u-hide-print" data-track="click" data-track-action="cite this article" data-track-label="link">Cite this article</a> </p> <div class="c-article-metrics-bar__wrapper u-clear-both"> <ul class="c-article-metrics-bar u-list-reset"> <li class=" c-article-metrics-bar__item" data-test="access-count"> <p class="c-article-metrics-bar__count">15k <span class="c-article-metrics-bar__label">Accesses</span></p> </li> <li class="c-article-metrics-bar__item" data-test="citation-count"> <p class="c-article-metrics-bar__count">11 <span class="c-article-metrics-bar__label">Citations</span></p> </li> <li class="c-article-metrics-bar__item" data-test="altmetric-score"> <p class="c-article-metrics-bar__count">80 <span class="c-article-metrics-bar__label">Altmetric</span></p> </li> <li class="c-article-metrics-bar__item"> <p class="c-article-metrics-bar__details"><a href="/articles/s41467-023-43718-w/metrics" data-track="click" data-track-action="view metrics" data-track-label="link" rel="nofollow">Metrics <span class="u-visually-hidden">details</span></a></p> </li> </ul> </div> </header> <div class="u-js-hide" data-component="article-subject-links"> <h3 class="c-article__sub-heading">Subjects</h3> <ul class="c-article-subject-list"> <li class="c-article-subject-list__subject"><a href="/subjects/peptides" data-track="click" data-track-action="view subject" data-track-label="link">Peptides</a></li><li class="c-article-subject-list__subject"><a href="/subjects/protein-design" data-track="click" data-track-action="view subject" data-track-label="link">Protein design</a></li><li class="c-article-subject-list__subject"><a href="/subjects/receptor-pharmacology" data-track="click" data-track-action="view subject" data-track-label="link">Receptor pharmacology</a></li> </ul> </div> </div> <div class="c-article-body"> <section aria-labelledby="Abs1" data-title="Abstract" lang="en"><div class="c-article-section" id="Abs1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Abs1">Abstract</h2><div class="c-article-section__content" id="Abs1-content"><p>Despite the increasing number of GPCR structures and recent advances in peptide design, the development of efficient technologies allowing rational design of high-affinity peptide ligands for single GPCRs remains an unmet challenge. Here, we develop a computational approach for designing conjugates of lariat-shaped macrocyclized peptides and a small molecule opioid ligand. We demonstrate its feasibility by discovering chemical scaffolds for the kappa-opioid receptor (KOR) with desired pharmacological activities. The designed De Novo Cyclic Peptide (DNCP)-β-naloxamine (NalA) exhibit in vitro potent mixed KOR agonism/mu-opioid receptor (MOR) antagonism, nanomolar binding affinity, selectivity, and efficacy bias at KOR. Proof-of-concept in vivo efficacy studies demonstrate that DNCP-β-NalA(1) induces a potent KOR-mediated antinociception in male mice. The high-resolution cryo-EM structure (2.6 Å) of the DNCP-β-NalA–KOR–Gi1 complex and molecular dynamics simulations are harnessed to validate the computational design model. This reveals a network of residues in ECL2/3 and TM6/7 controlling the intrinsic efficacy of KOR. In general, our computational de novo platform overcomes extensive lead optimization encountered in ultra-large library docking and virtual small molecule screening campaigns and offers innovation for GPCR ligand discovery. This may drive the development of next-generation therapeutics for medical applications such as pain conditions.</p></div></div></section> <noscript> </noscript> <section aria-labelledby="inline-recommendations" data-title="Inline Recommendations" class="c-article-recommendations" data-track-component="inline-recommendations"> <h3 class="c-article-recommendations-title" id="inline-recommendations">Similar content being viewed by others</h3> <div class="c-article-recommendations-list"> <div class="c-article-recommendations-list__item"> <article class="c-article-recommendations-card" itemscope itemtype="http://schema.org/ScholarlyArticle"> <div class="c-article-recommendations-card__img"><img src="https://media.springernature.com/w215h120/springer-static/image/art%3A10.1038%2Fs41586-022-05588-y/MediaObjects/41586_2022_5588_Fig1_HTML.png" loading="lazy" alt=""></div> <div class="c-article-recommendations-card__main"> <h3 class="c-article-recommendations-card__heading" itemprop="name headline"> <a class="c-article-recommendations-card__link" itemprop="url" href="https://www.nature.com/articles/s41586-022-05588-y?fromPaywallRec=false" data-track="select_recommendations_1" data-track-context="inline recommendations" data-track-action="click recommendations inline - 1" data-track-label="10.1038/s41586-022-05588-y">Structure-based design of bitopic ligands for the µ-opioid receptor </a> </h3> <div class="c-article-meta-recommendations" data-test="recommendation-info"> <span class="c-article-meta-recommendations__item-type">Article</span> <span class="c-article-meta-recommendations__date">30 November 2022</span> </div> </div> </article> </div> <div class="c-article-recommendations-list__item"> <article class="c-article-recommendations-card" itemscope itemtype="http://schema.org/ScholarlyArticle"> <div class="c-article-recommendations-card__img"><img src="https://media.springernature.com/w215h120/springer-static/image/art%3A10.1038%2Fs41467-023-37041-7/MediaObjects/41467_2023_37041_Fig1_HTML.png" loading="lazy" alt=""></div> <div class="c-article-recommendations-card__main"> <h3 class="c-article-recommendations-card__heading" itemprop="name headline"> <a class="c-article-recommendations-card__link" itemprop="url" href="https://www.nature.com/articles/s41467-023-37041-7?fromPaywallRec=false" data-track="select_recommendations_2" data-track-context="inline recommendations" data-track-action="click recommendations inline - 2" data-track-label="10.1038/s41467-023-37041-7">Molecular mechanism of biased signaling at the kappa opioid receptor </a> </h3> <div class="c-article-meta-recommendations" data-test="recommendation-info"> <span class="c-article-meta-recommendations__item-type">Article</span> <span class="c-article-meta-recommendations__access-type">Open access</span> <span class="c-article-meta-recommendations__date">11 March 2023</span> </div> </div> </article> </div> <div class="c-article-recommendations-list__item"> <article class="c-article-recommendations-card" itemscope itemtype="http://schema.org/ScholarlyArticle"> <div class="c-article-recommendations-card__img"><img src="https://media.springernature.com/w215h120/springer-static/image/art%3A10.1038%2Fs41467-024-52947-6/MediaObjects/41467_2024_52947_Fig1_HTML.png" loading="lazy" alt=""></div> <div class="c-article-recommendations-card__main"> <h3 class="c-article-recommendations-card__heading" itemprop="name headline"> <a class="c-article-recommendations-card__link" itemprop="url" href="https://www.nature.com/articles/s41467-024-52947-6?fromPaywallRec=false" data-track="select_recommendations_3" data-track-context="inline recommendations" data-track-action="click recommendations inline - 3" data-track-label="10.1038/s41467-024-52947-6">Structural basis of μ-opioid receptor targeting by a nanobody antagonist </a> </h3> <div class="c-article-meta-recommendations" data-test="recommendation-info"> <span class="c-article-meta-recommendations__item-type">Article</span> <span class="c-article-meta-recommendations__access-type">Open access</span> <span class="c-article-meta-recommendations__date">09 October 2024</span> </div> </div> </article> </div> </div> </section> <script> window.dataLayer = window.dataLayer || []; window.dataLayer.push({ recommendations: { recommender: 'semantic', model: 'specter', policy_id: 'NA', timestamp: 1732370363, embedded_user: 'null' } }); </script> <div class="main-content"> <section data-title="Introduction"><div class="c-article-section" id="Sec1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec1">Introduction</h2><div class="c-article-section__content" id="Sec1-content"><p>G protein-coupled receptors (GPCRs) are critical therapeutic targets for analgesics, antihistamines, neuroleptics, and many cardiovascular drugs<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1" title="Davenport, A. P., Scully, C. C. G., de Graaf, C., Brown, A. J. H. & Maguire, J. J. Advances in therapeutic peptides targeting G protein-coupled receptors. Nat. Rev. Drug Discov. 19, 389–413 (2020)." href="/articles/s41467-023-43718-w#ref-CR1" id="ref-link-section-d112540969e1022">1</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2" title="Hauser, A. S., Attwood, M. M., Rask-Andersen, M., Schioth, H. B. & Gloriam, D. E. Trends in GPCR drug discovery: new agents, targets and indications. Nat. Rev. Drug Discov. 16, 829–842 (2017)." href="/articles/s41467-023-43718-w#ref-CR2" id="ref-link-section-d112540969e1025">2</a></sup>. Small molecules are a common therapeutic modality for targeting GPCRs<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2" title="Hauser, A. S., Attwood, M. M., Rask-Andersen, M., Schioth, H. B. & Gloriam, D. E. Trends in GPCR drug discovery: new agents, targets and indications. Nat. Rev. Drug Discov. 16, 829–842 (2017)." href="/articles/s41467-023-43718-w#ref-CR2" id="ref-link-section-d112540969e1029">2</a></sup> due to their low cost, high stability, lipophilicity, and oral bioavailability; however, they often have limited target selectivity and are associated with off-target effects and adverse clinical events<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 3" title="Muratspahic, E., Freissmuth, M. & Gruber, C. W. Nature-derived peptides: a growing niche for GPCR ligand discovery. Trends Pharmacol. Sci. 40, 309–326 (2019)." href="/articles/s41467-023-43718-w#ref-CR3" id="ref-link-section-d112540969e1033">3</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 4" title="Muttenthaler, M., King, G. F., Adams, D. J. & Alewood, P. F. Trends in peptide drug discovery. Nat. Rev. Drug Discov. 20, 309–325 (2021)." href="/articles/s41467-023-43718-w#ref-CR4" id="ref-link-section-d112540969e1036">4</a></sup>. A prominent example is the ongoing and rapidly evolving global opioid crisis accompanied by substantial opioid-related morbidity and mortality<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 5" title="Volkow, N. D. & Collins, F. S. The role of science in addressing the opioid crisis. N. Engl. J. Med. 377, 391–394 (2017)." href="/articles/s41467-023-43718-w#ref-CR5" id="ref-link-section-d112540969e1040">5</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 6" title="Darcq, E. & Kieffer, B. L. Opioid receptors: drivers to addiction? Nat. Rev. Neurosci. 19, 499–514 (2018)." href="/articles/s41467-023-43718-w#ref-CR6" id="ref-link-section-d112540969e1043">6</a></sup>. Prescribed opioid analgesics including fentanyl, morphine and their derivatives, that act primarily via the mu-opioid receptor (MOR), have numerous and serious side effects<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 7" title="Del Vecchio, G., Spahn, V. & Stein, C. Novel opioid analgesics and side effects. ACS Chem. Neurosci. 8, 1638–1640 (2017)." href="/articles/s41467-023-43718-w#ref-CR7" id="ref-link-section-d112540969e1047">7</a></sup>. The kappa-opioid receptor (KOR) is an attractive alternative of therapeutic value for the development of non-addictive pain relievers<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e1052">8</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 9" title="Che, T., Dwivedi-Agnihotri, H., Shukla, A. K. & Roth, B. L. Biased ligands at opioid receptors: current status and future directions. Sci. Signal. 14, aav0320 (2021)." href="/articles/s41467-023-43718-w#ref-CR9" id="ref-link-section-d112540969e1055">9</a></sup>. Nevertheless, while KOR is a promising target, its activation can result in other undesired effects, including psychotomimessis, sedation and dysphoria<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 10" title="Liu-Chen, L. Y. & Huang, P. Signaling underlying kappa opioid receptor-mediated behaviors in rodents. Front. Neurosci. 16, 964724 (2022)." href="/articles/s41467-023-43718-w#ref-CR10" id="ref-link-section-d112540969e1059">10</a></sup>, which has limited the clinical development of KOR agonists as analgesic drugs<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 9" title="Che, T., Dwivedi-Agnihotri, H., Shukla, A. K. & Roth, B. L. Biased ligands at opioid receptors: current status and future directions. Sci. Signal. 14, aav0320 (2021)." href="/articles/s41467-023-43718-w#ref-CR9" id="ref-link-section-d112540969e1063">9</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 11" title="Machelska, H. & Celik, M. O. Advances in achieving opioid analgesia without side effects. Front. Pharmacol. 9, 1388 (2018)." href="/articles/s41467-023-43718-w#ref-CR11" id="ref-link-section-d112540969e1066">11</a></sup>. Intensive research over the past decade has thus focused on the design of molecular scaffolds targeting KOR with improved side effect profiles. For instance, functionally selective small molecule KOR ligands, partial KOR agonists and mixed KOR/MOR agonists, exhibited fewer side effects in preclinical settings<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 9" title="Che, T., Dwivedi-Agnihotri, H., Shukla, A. K. & Roth, B. L. Biased ligands at opioid receptors: current status and future directions. Sci. Signal. 14, aav0320 (2021)." href="/articles/s41467-023-43718-w#ref-CR9" id="ref-link-section-d112540969e1070">9</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 11" title="Machelska, H. & Celik, M. O. Advances in achieving opioid analgesia without side effects. Front. Pharmacol. 9, 1388 (2018)." href="/articles/s41467-023-43718-w#ref-CR11" id="ref-link-section-d112540969e1073">11</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 12" title="Dalefield, M. L., Scouller, B., Bibi, R. & Kivell, B. M. The kappa opioid receptor: a promising therapeutic target for multiple pathologies. Front. Pharmacol. 13, 837671 (2022)." href="/articles/s41467-023-43718-w#ref-CR12" id="ref-link-section-d112540969e1076">12</a></sup>. However, the utility of functionally selective (biased) ligands of opioid receptors remains controversial<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 13" title="Bachmutsky, I., Wei, X. P., Durand, A. & Yackle, K. β-arrestin 2 germline knockout does not attenuate opioid respiratory depression. Elife 10, 
 https://doi.org/10.7554/eLife.62552
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR13" id="ref-link-section-d112540969e1080">13</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 14" title="Kliewer, A. et al. Phosphorylation-deficient G-protein-biased mu-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat. Commun. 10, 367 (2019)." href="/articles/s41467-023-43718-w#ref-CR14" id="ref-link-section-d112540969e1083">14</a></sup>. Hence, there is an unmet need to discover KOR ligands that display unique pharmacological properties and diminished unwanted effects. Previously, this has been attempted using high-throughput small molecule library screening<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 15" title="Frankowski, K. J. et al. Discovery of small molecule kappa opioid receptor agonist and antagonist chemotypes through a HTS and hit refinement strategy. ACS Chem. Neurosci. 3, 221–236 (2012)." href="/articles/s41467-023-43718-w#ref-CR15" id="ref-link-section-d112540969e1087">15</a></sup>, virtual small molecule screening<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 16" title="Negri, A. et al. Discovery of a novel selective kappa-opioid receptor agonist using crystal structure-based virtual screening. J. Chem. Inf. Model 53, 521–526 (2013)." href="/articles/s41467-023-43718-w#ref-CR16" id="ref-link-section-d112540969e1092">16</a></sup> or natural product discovery<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 17" title="Roth, B. L. et al. Salvinorin A: a potent naturally occurring nonnitrogenous kappa opioid selective agonist. Proc. Natl Acad. Sci. USA 99, 11934–11939 (2002)." href="/articles/s41467-023-43718-w#ref-CR17" id="ref-link-section-d112540969e1096">17</a></sup>, but with limited translational success. In parallel, advances in computational design have enabled the design of structured cyclic peptides and small proteins with high affinity and selectivity for a variety of targets<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" title="Bhardwaj, G. et al. Accurate de novo design of hyperstable constrained peptides. Nature 538, 329–335 (2016)." href="#ref-CR18" id="ref-link-section-d112540969e1100">18</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" title="Cao, L. et al. De novo design of picomolar SARS-CoV-2 miniprotein inhibitors. Science 370, 426–431 (2020)." href="#ref-CR19" id="ref-link-section-d112540969e1100_1">19</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" title="Chevalier, A. et al. Massively parallel de novo protein design for targeted therapeutics. Nature 550, 74–79 (2017)." href="#ref-CR20" id="ref-link-section-d112540969e1100_2">20</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" title="Hosseinzadeh, P. et al. Comprehensive computational design of ordered peptide macrocycles. Science 358, 1461–1466 (2017)." href="#ref-CR21" id="ref-link-section-d112540969e1100_3">21</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" title="Hosseinzadeh, P. et al. Anchor extension: a structure-guided approach to design cyclic peptides targeting enzyme active sites. Nat. Commun. 12, 3384 (2021)." href="#ref-CR22" id="ref-link-section-d112540969e1100_4">22</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 23" title="Mulligan, V. K. et al. Computationally designed peptide macrocycle inhibitors of New Delhi metallo-beta-lactamase 1. Proc. Natl Acad. Sci. USA 118, e2012800118 (2021)." href="/articles/s41467-023-43718-w#ref-CR23" id="ref-link-section-d112540969e1103">23</a></sup>. Despite this progress, there have been few successes in structure-guided peptide design for GPCRs, mainly due to the lack of high-resolution active-state GPCR structures<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 24" title="Danev, R. et al. Routine sub-2.5 Å cryo-EM structure determination of GPCRs. Nat. Commun. 12, 4333 (2021)." href="/articles/s41467-023-43718-w#ref-CR24" id="ref-link-section-d112540969e1107">24</a></sup> and the recessed ligand binding pockets of many small molecule sensing GPCRs which can be difficult for peptides to access.</p><p>We reason that the advantages of small molecule ligands in penetrating deeply into GPCR binding clefts and of peptides in making more extensive target interactions for specificity could be combined by computational design of peptide–small molecule conjugates. Utilizing a small molecule ligand extended by a peptide moiety allows access simultaneously to orthosteric and alternative binding sites of GPCRs, enabling the modulation of different active states of the receptor<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 25" title="Hedderich, J. B. et al. The pocketome of G protein-coupled receptors reveals previously untargeted allosteric sites. Nat. Commun. 13, 2567 (2022)." href="/articles/s41467-023-43718-w#ref-CR25" id="ref-link-section-d112540969e1114">25</a></sup>, which could endow such ligands with unique pharmacological properties.</p><p>Here, we exploit the crystal structure of KOR bound to the dual KOR/delta-opioid receptor (DOR) epoxymorphinan opioid agonist MP1104<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e1121">8</a></sup> and use the Rosetta protein and peptide design software to computationally design peptide–small molecule conjugates targeting KOR. Utilizing the high affinity interaction of MP1104<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 26" title="Varadi, A. et al. Synthesis and characterization of a dual kappa-delta opioid receptor agonist analgesic blocking cocaine reward behavior. ACS Chem. Neurosci. 6, 1813–1824 (2015)." href="/articles/s41467-023-43718-w#ref-CR26" id="ref-link-section-d112540969e1125">26</a></sup> with KOR as an anchor to initiate the design calculations, we seek to computationally design thioether cyclic peptides that interact with ECL2 and ECL3 of the receptor<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 27" title="Majumdar, S. et al. Synthesis and evaluation of aryl-naloxamide opiate analgesics targeting truncated exon 11-associated mu opioid receptor (MOR-1.) splice variants. J. Med. Chem. 55, 6352–6362 (2012)." href="/articles/s41467-023-43718-w#ref-CR27" id="ref-link-section-d112540969e1129">27</a></sup>.</p></div></div></section><section data-title="Results"><div class="c-article-section" id="Sec2-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec2">Results</h2><div class="c-article-section__content" id="Sec2-content"><h3 class="c-article__sub-heading" id="Sec3">Computational design of KOR-targeted peptide–small molecule conjugates</h3><p>We developed a computational design approach using Rosetta peptide design methods<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 18" title="Bhardwaj, G. et al. Accurate de novo design of hyperstable constrained peptides. Nature 538, 329–335 (2016)." href="/articles/s41467-023-43718-w#ref-CR18" id="ref-link-section-d112540969e1145">18</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 21" title="Hosseinzadeh, P. et al. Comprehensive computational design of ordered peptide macrocycles. Science 358, 1461–1466 (2017)." href="/articles/s41467-023-43718-w#ref-CR21" id="ref-link-section-d112540969e1148">21</a></sup> to design high affinity cyclic peptide–small molecule drug conjugate ligands targeting KOR (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig1">1</a>). The recently published structure of human KOR bound to small molecule MP1104 (<a href="https://doi.org/10.2210/pdb6B73/pdb">PDB: 6B73</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e1162">8</a></sup>, reveals multiple interactions between MP1104 and KOR (e.g., T111<sup>2.56</sup>, F114<sup>2.59</sup>, Q115<sup>2.60</sup>, Y139<sup>3.33</sup> and V230<sup>5.43</sup>) that contribute to binding (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">1</a>). We reasoned that a small molecule ligand resembling MP1104 could provide affinity for KOR, and that selectivity and efficacy could be modulated by a conjugated cyclic peptide interacting with the extracellular loops. To enable chemical synthesis of this conjugate, we decided to use lariat peptides cyclized through a sidechain and the N-terminus, with the C-terminus thus available for covalent modification. The cyclic motif embedded within the peptide sequence reduces flexibility and thus reduces the entropy loss upon binding which can increase binding affinity and enhance stability<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 4" title="Muttenthaler, M., King, G. F., Adams, D. J. & Alewood, P. F. Trends in peptide drug discovery. Nat. Rev. Drug Discov. 20, 309–325 (2021)." href="/articles/s41467-023-43718-w#ref-CR4" id="ref-link-section-d112540969e1180">4</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 28" title="Wang, C. K., Swedberg, J. E., Northfield, S. E. & Craik, D. J. Effects of cyclization on peptide backbone dynamics. J. Phys. Chem. B 119, 15821–15830 (2015)." href="/articles/s41467-023-43718-w#ref-CR28" id="ref-link-section-d112540969e1183">28</a></sup>. Based on the size and shape of the binding pocket, we chose to employ the cyclic component of the lariat 5-6 residues closed by thioether macrocyclization linking a Cys side chain and the N-terminus (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig1">1b</a>).</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-1" data-title="Strategy for the computational design of thioether macrocyclized peptide–small molecule conjugates targeting KOR."><figure><figcaption><b id="Fig1" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 1: Strategy for the computational design of thioether macrocyclized peptide–small molecule conjugates targeting KOR.</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/1" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig1_HTML.png?as=webp"><img aria-describedby="Fig1" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig1_HTML.png" alt="figure 1" loading="lazy" width="685" height="550"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-1-desc"><p><b>a</b> 3D human KOR structure with small molecule agonist MP1104 was used as the starting template (<a href="https://doi.org/10.2210/pdb6B73/pdb">PDB: 6B73</a>). <b>b</b> Workflows for computational peptide–small molecule conjugate design: (1) Measurement of the pocket area (934 Å3) narrowed down the size of macrocycles to focus on 5- and 6-mer cyclic peptides. (2) Generation of small molecules with two additional amino acids, which were sampled and scored for optimal dimer sequence (select dipeptide modified small molecules: Gray: <span class="u-monospace">CVV-D-Phe-Thr</span>; Yellow: <span class="u-monospace">CVV-D-Phe-Gln</span>; Orange: <span class="u-monospace">CVV-D-Phe-Ser</span>; CVV corresponds to N-cyclopropylmethyl-epoxy morphinan small molecule stub). (3) Generation of a comprehensive library of 5- and 6-mer thioether cyclized peptides clustered via torsion angle and hydrogen bond pattern. (4) Docked structure of thioether macrocyclized hexamers through coordinate-guided transformation of the backbone C-termini to the generated anchor N-termini. (5) Rotamer design to optimize the interface interactions of the backbones. (6) Design filtering based on shape complementarity and interface area as representative examples for interface metrics; dashed red line represents 90th percentile cut-off values.</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/1" data-track-dest="link:Figure1 Full size image" aria-label="Full size image figure 1" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div><p>We started from a variant of MP1104 lacking the iodobenzamide group—N-cyclopropylmethyl- epoxy morphinan—bound to the KOR structure in the same orientation as MP1104. This MP1104 derivative has a free amine, which can be conjugated to the C-terminus of a peptide lariat. We first modeled two amino acid residues extending off the free amino group of the MP1104 derivative, and extensively sampled their backbone torsion angles. Next to the free amino group, we placed a D-phenylalanine to mimic the MP1104 iodobenzamide group, and at the second position we sampled all 20 amino acids (excluding glycine and cysteine) in L and D forms, aiming for interactions with the extracellular loops of the receptor (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">1c, d</a>). Over all combinations of backbone conformations and amino acid possibilities, we chose four solutions (i.e., dipeptides D-Phe-L-Thr; D-Phe-L-Ser; D-Phe-L-Gln and D-Phe-L-Ala) with the lowest Rosetta binding energy for KOR that were next used to graft 5- and 6-mers thioether cyclized peptides onto.</p><p>We next generated a library of thioether cyclized peptides to graft onto the di-peptide-morphinan models. We used Rosetta to generate a large set of cyclic thioether peptide scaffolds with 5 or 6 amino acids using backbone generation and clustering protocols previously employed to generate backbone cyclized peptides (Supplementary Data <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM4">1</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 21" title="Hosseinzadeh, P. et al. Comprehensive computational design of ordered peptide macrocycles. Science 358, 1461–1466 (2017)." href="/articles/s41467-023-43718-w#ref-CR21" id="ref-link-section-d112540969e1244">21</a></sup>. The thioether scaffolds were fused to the generated tails by coordinate transformations to form amide bonds between the C-termini of the thioether cyclic peptide backbones and the N-termini of the tail segments (Supplementary Data <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM5">2</a>). We performed iterative sequence design rounds on the peptide backbone to optimize interactions with the KOR pocket using the Rosetta FastDesign protocol (Supplementary Data <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM6">3</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 18" title="Bhardwaj, G. et al. Accurate de novo design of hyperstable constrained peptides. Nature 538, 329–335 (2016)." href="/articles/s41467-023-43718-w#ref-CR18" id="ref-link-section-d112540969e1254">18</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 20" title="Chevalier, A. et al. Massively parallel de novo protein design for targeted therapeutics. Nature 550, 74–79 (2017)." href="/articles/s41467-023-43718-w#ref-CR20" id="ref-link-section-d112540969e1257">20</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 21" title="Hosseinzadeh, P. et al. Comprehensive computational design of ordered peptide macrocycles. Science 358, 1461–1466 (2017)." href="/articles/s41467-023-43718-w#ref-CR21" id="ref-link-section-d112540969e1260">21</a></sup>. After each round of design, the computed free energy of binding (∆∆G), the shape complementarity, and the interface area were assessed, and a 90th percentile cut-off on all three properties was used to filter the designs down to 50 structures (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig1">1</a>, Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">2</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 20" title="Chevalier, A. et al. Massively parallel de novo protein design for targeted therapeutics. Nature 550, 74–79 (2017)." href="/articles/s41467-023-43718-w#ref-CR20" id="ref-link-section-d112540969e1271">20</a></sup>. The designs were further filtered based on interactions with the extracellular loops (ECL2 and/or ECL3) (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig2">2a</a>) and the diversity of the sequences, shape of the cyclic backbone, and extracellular loop interactions across ECL2/3 (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig2">2b</a>). Six thioether cyclized hexamer conjugates were selected for synthesis (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig2">2c</a>).</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-2" data-title="Overview of the selected peptide macrocycle designs and the synthetic strategy to produce the peptide–small molecule conjugates and controls."><figure><figcaption><b id="Fig2" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 2: Overview of the selected peptide macrocycle designs and the synthetic strategy to produce the peptide–small molecule conjugates and controls.</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/2" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig2_HTML.png?as=webp"><img aria-describedby="Fig2" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig2_HTML.png" alt="figure 2" loading="lazy" width="685" height="648"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-2-desc"><p><b>a</b> Peptides have interactions with ECL2 and/or ECL3 of KOR with a subset overlay of the peptide backbone designs (aligned by the small molecule fragment anchor) showing shape diversity in the pocket. <b>b</b> Final selection of peptide sequences. Instead of focusing on multiple sequences for a single promising backbone, we sought to select designs across diverse shapes and sequences for experimental testing. <b>c</b> Synthetic scheme depicting solid phase synthesis of the de novo linear peptides (DNLP) (<b>11</b>–<b>16</b>) and the de novo cyclic peptides (DNCP) (<b>21</b>–<b>26</b> and <b>31</b>–<b>36</b>) and the solution phase conjugation reaction with β-naloxamine (β-NalA) to generate the DNCP-β-NalA (<b>1</b>–<b>6</b>) conjugates. R<sub>#</sub> indicates a side chain of the respective amino acid. PG denotes protecting groups. Rink amide resin was used for synthesis of DNLP (<b>11</b>–<b>16</b>) and DNCP (<b>21</b>–<b>26</b>), whereas Fmoc-D-Phe preloaded Wang resin was used for DNCP (<b>31–36</b>) synthesis. The amino acids indicated by R<sub>#</sub> in Fig. 2b correspond to the identical side chain represented by R<sub>#</sub> in Fig. 2c.</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/2" data-track-dest="link:Figure2 Full size image" aria-label="Full size image figure 2" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div><h3 class="c-article__sub-heading" id="Sec4">Chemical synthesis of the designed ligands and determination of their pharmacodynamic properties at KOR</h3><p>We first synthesized de novo linear (DNLP) and de novo cyclic peptides (DNCP) (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig2">2c</a> and Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3a</a>) to test if they can bind to the orthosteric site of KOR on their own to validate the conjugate design. Hence, DNLPs (<b>11</b>–<b>16</b>) were synthesized by Fmoc-solid phase peptide synthesis (Fmoc-SPPS) and purified by RP-HPLC (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">4</a>, Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">1</a> and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">2</a>). The DNCPs (<b>21</b>–<b>26</b>) were also assembled by Fmoc-SPPS, followed by a three-step procedure consisting of N-terminal on-resin bromoacetylation, chemoselective Mmt deprotection of Cys<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 6" title="Darcq, E. & Kieffer, B. L. Opioid receptors: drivers to addiction? Nat. Rev. Neurosci. 19, 499–514 (2018)." href="/articles/s41467-023-43718-w#ref-CR6" id="ref-link-section-d112540969e1397">6</a></sup>, and a final on-resin thioether cyclization to form the 6-residue macrocycle (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">5</a>, Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">1</a> and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">2</a>). Then DNLPs (<b>11</b>–<b>16</b>) and DNCPs (<b>21</b>–<b>26</b>) were assayed in one-point radioligand binding assays using membrane preparations from HEK293 cells stably expressing the mouse KOR. None of the tested peptides was able to displace the orthosteric antagonist [<sup>3</sup>H]diprenorphine ([<sup>3</sup>H]DPN) from KOR at a concentration of 10 µM (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">6</a>).</p><p>For synthesis of the peptide–drug conjugates, we chose β-NalA, distinguished by a less rigid morphinan structure and featuring an N-17 allyl group over the N-cyclopropylmethyl group of the MP1104 derivative (i.e., N-cyclopropylmethyl-epoxy morphinan), which was utilized during computational design. This decision was guided by the closely resembling core structure, along with the benefits of β-NalA’s simpler synthesis and a more flexible morphinan ring, thereby allowing the peptide region to bind the ECL2 region of KOR more tightly<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 29" title="Uprety, R. et al. Controlling opioid receptor functional selectivity by targeting distinct subpockets of the orthosteric site. Elife 10, 
 https://doi.org/10.7554/eLife.56519
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR29" id="ref-link-section-d112540969e1434">29</a></sup>. Conjugation of the DNCPs (<b>31</b>–<b>36</b>) (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">7</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">2</a>) to β-NalA was achieved by coupling the C-terminal carboxylic acid (COOH) of the DNCPs to the primary amino group (NH<sub>2</sub>) of β-NalA (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig2">2c</a> and Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). We obtained four DNCP-β-NalA conjugates (<b>1</b>–<b>4</b>) (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">8</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">2</a>); the conjugation of DNCP (<b>35</b>) and DNCP (<b>36</b>) was unsuccessful (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">9</a>). DNCP-β-NalA conjugates (<b>1</b>–<b>4</b>) were pharmacologically characterized via radioligand binding and functional cAMP assays to investigate their affinity, potency and efficacy at the mouse KOR (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3a, b</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). The DNCP-β-NalA conjugates exhibited affinities in the low nanomolar range (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>), with DNCP-β-NalA(<b>1</b>) being the strongest binder at KOR with a <i>K</i><sub>i</sub> value of 3.9 nM (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3a</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>), as compared to β-NalA, which has an affinity <i>K</i><sub>i</sub> of 72 nM. All four DNCP-β-NalA conjugates were full agonists at KOR (<i>E</i><sub>max</sub> = 89–101%), while β-NalA alone was only a partial agonist with an <i>EC</i><sub>50</sub> of 130 nM and <i>E</i><sub>max</sub> of 61% (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3b</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). The most potent conjugates were DNCP-β-NalA(<b>1</b>) and (<b>4</b>) with <i>EC</i><sub>50</sub> values of 2.0 nM and 1.0 nM, respectively (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3b</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). DNCP-β-NalA(<b>2</b>) and DNCP-β-NalA(<b>3</b>) showed agonist activities at KOR with <i>EC</i><sub>50</sub> values of 7.5 and 14 nM in cAMP assay, respectively, while their <i>K</i><sub>i</sub> values were 31 and 24 nM in radioligand binding assay, respectively (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). Receptor reserve may account for this discrepancy between potency and affinity values of DNCP-β-NalA(<b>2</b>) and DNCP-β-NalA(<b>4</b>) which can be observed in functional GPCR assays with opioid receptors<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 30" title="Kelly, E. Efficacy and ligand bias at the mu-opioid receptor. Br. J. Pharmacol. 169, 1430–1446 (2013)." href="/articles/s41467-023-43718-w#ref-CR30" id="ref-link-section-d112540969e1576">30</a></sup>. The higher potency and efficacy of the conjugates at KOR compared to β-NalA can be attributed to interactions of the macrocycles with the ECL2 region as described later.</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-3" data-title="In vitro receptor pharmacology of peptide–small molecule conjugates."><figure><figcaption><b id="Fig3" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 3: In vitro receptor pharmacology of peptide–small molecule conjugates.</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/3" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig3_HTML.png?as=webp"><img aria-describedby="Fig3" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig3_HTML.png" alt="figure 3" loading="lazy" width="685" height="538"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-3-desc"><p><b>a</b>, <b>b</b> Radioligand binding (<i>n</i> = 3) and functional cAMP assays (<i>n</i> = 3–4) of DNCP-β-NalA conjugates (<b>1</b>–<b>4</b>) were performed on HEK293T cell membranes stably expressing mouse KOR. Binding (<b>a</b>) was measured by displacing 1 nM of [<sup>3</sup>H]DPN whereas cAMP inhibition (<b>b</b>) was monitored after treatment with indicated concentrations of conjugates. U50,488 and β-NalA were positive controls. Final concentration of 10 µM of forskolin was used to stimulate cAMP production (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). <b>c</b> Concentration-dependent stimulation of [<sup>35</sup>S]GTPγS binding by the most potent DNCP-β-NalA(<b>1</b>) (<i>n</i> = 3), β-NalA (<i>n</i> = 3), U69,593 (<i>n</i> = 3) and dyn A<sub>1-13</sub> (<i>n</i> = 4) in human KOR expressing CHO cell membranes (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">4</a>). <b>d</b> β-arrestin-2 recruitment assay of DNCP-β-NalA(<b>1</b>), β-NalA and dynorphin (dyn) A<sub>1-13</sub> was done in HEK293T cells transiently expressing mouse KOR-EGFP and β-arrestin-2-nano-luciferase (<i>n</i> = 3–6) (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). <b>e</b> <i>α</i>-Subtype screening of DNCP-β-NalA(<b>1</b>) at the mouse KOR in the TRUPATH assay (<i>n</i> = 8) (Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">5</a> and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">6</a>). <b>f</b> Selectivity of DNCP-β-NalA(<b>1</b>) was determined in a radioligand binding assay using HEK293T cell membrane preparations stably expressing mouse MOR and DOR and 1 nM of [<sup>3</sup>H]DPN, respectively (<i>n</i> = 3). <b>g</b>, <b>h</b> Gα<sub>i</sub>-mediated cAMP inhibition of DNCP-β-NalA(<b>1</b>) at the mouse MOR (<b>g</b>) and DOR (<b>h</b>) was measured in stable HEK293T cells using DAMGO and DADLE as reference ligands, respectively (<i>n</i> = 3). <b>i</b>, <b>j</b> Spider plots from TRUPATH (<i>n</i> = 8 for each Gα), β-arrestin-1 (<i>n</i> = 4) and β-arrestin-2 (<i>n</i> = 3–6) recruitment assays represent potency (log <i>EC</i><sub>50</sub>) (<b>i</b>) and normalized efficacy (<b>j</b>) of DNCP-β-NalA(<b>1</b>), U50,488, MP1104, pentazocine, β-NalA and dyn A<sub>1-13</sub>. Data were normalized to full KOR agonists U50,488, U69,593 or dyn A<sub>1-13</sub>, full MOR agonist DAMGO and full DOR agonist DADLE. All data are presented as mean values ± s.e.m. Source data are provided as a Source Data file.</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/3" data-track-dest="link:Figure3 Full size image" aria-label="Full size image figure 3" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div><h3 class="c-article__sub-heading" id="Sec5">Pharmacological profiling of DNCP-β-NalA(1) for receptor subtype selectivity and functional bias</h3><p>We pursued more detailed pharmacological characterization of DNCP-β-NalA(<b>1</b>) to obtain information regarding the affinity and activation at MOR and DOR, as well as the potency/efficacy of G proteins vs. β-arrestins. Given the sequence differences between human and mouse KOR (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">10</a>) we evaluated the in vitro functional activity of DNCP-β-NalA(<b>1</b>) at the human KOR in the [<sup>35</sup>S]GTPγS binding assay using CHO cell membrane preparations stably expressing human KOR. DNCP-β-NalA(<b>1</b>) fully activated human KOR (<i>EC</i><sub>50</sub> = 5.5 nM; <i>E</i><sub>max</sub> = 83%) compared to the partial agonist β-NalA (<i>EC</i><sub>50</sub> = 150 nM; <i>E</i><sub>max</sub> = 22%) and the reference KOR agonists U69,593 and dynorphin (dyn) A<sub>1-13</sub> (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3c</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">4</a>), which is in agreement with the activation of the mouse KOR as determined by cAMP quantification (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3b</a>).</p><p>Pathway-selective KOR ligands offer great potential not only as molecular probes to dissect receptor pharmacology but also as potential non-addictive next-generation analgesics. In this context, β-arrestins have been linked to severe adverse effects, thereby highlighting the potential of G protein-biased ligands to design pain relievers with improved side effect profiles<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 9" title="Che, T., Dwivedi-Agnihotri, H., Shukla, A. K. & Roth, B. L. Biased ligands at opioid receptors: current status and future directions. Sci. Signal. 14, aav0320 (2021)." href="/articles/s41467-023-43718-w#ref-CR9" id="ref-link-section-d112540969e1810">9</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 31" title="Brust, T. F. et al. Biased agonists of the kappa opioid receptor suppress pain and itch without causing sedation or dysphoria. Sci. Signal. 9, aai8441 (2016)." href="/articles/s41467-023-43718-w#ref-CR31" id="ref-link-section-d112540969e1813">31</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 32" title="Faouzi, A., Varga, B. R. & Majumdar, S. Biased opioid ligands. Molecules 25, 
 https://doi.org/10.3390/molecules25184257
 
 (2020)." href="/articles/s41467-023-43718-w#ref-CR32" id="ref-link-section-d112540969e1816">32</a></sup>. Thus, we explored the ability of DNCP-β-NalA(<b>1</b>) to recruit β-arrestin-2 at KOR in a bioluminescence resonance energy transfer (BRET) assay (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3d, i, j</a>). In contrast to reference ligands dynorphin (dyn) A<sub>1-13</sub> and U69,593 (full agonists of β-arrestin-2, with an <i>EC</i><sub>50</sub> of 31 and 500 nM, respectively) DNCP-β-NalA(<b>1</b>) only partially recruited β-arrestin-2 with an <i>E</i><sub>max</sub> of 41% and an <i>EC</i><sub>50</sub> of 22 nM (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3d</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). Similarly, β-NalA recruited β-arrestin-2 at KOR with an <i>E</i><sub>max</sub> and an <i>EC</i><sub>50</sub> of 51% and 15 nM, respectively. DNCP-β-NalA(<b>1</b>) also partially recruited β-arrestin-1 at KOR with an <i>E</i><sub>max</sub> of 30% and <i>EC</i><sub>50</sub> of 28 nM (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3i, j</a>, Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">11a</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">3</a>). Hence, DNCP-β-NalA(<b>1</b>) demonstrated impaired β-arrestin recruitment, established to be associated with reduced side effects<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 31" title="Brust, T. F. et al. Biased agonists of the kappa opioid receptor suppress pain and itch without causing sedation or dysphoria. Sci. Signal. 9, aai8441 (2016)." href="/articles/s41467-023-43718-w#ref-CR31" id="ref-link-section-d112540969e1884">31</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 33" title="Bohn, L. M. & Aube, J. Seeking (and Finding) biased ligands of the kappa opioid receptor. ACS Med. Chem. Lett. 8, 694–700 (2017)." href="/articles/s41467-023-43718-w#ref-CR33" id="ref-link-section-d112540969e1887">33</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 34" title="Paton, K. F., Atigari, D. V., Kaska, S., Prisinzano, T. & Kivell, B. M. Strategies for developing kappa opioid receptor agonists for the treatment of pain with fewer side. Effects. J. Pharmacol. Exp. Ther. 375, 332–348 (2020)." href="/articles/s41467-023-43718-w#ref-CR34" id="ref-link-section-d112540969e1890">34</a></sup>. We then profiled DNCP-β-NalA(<b>1</b>) in the TRUPATH assay to interrogate their Gα<sub>i/o</sub> coupling preferences Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3e, i, j</a>, Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">11b–d</a>, Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">5</a> and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">6</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 35" title="Olsen, R. H. J. et al. TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat. Chem. Biol. 16, 841–849 (2020)." href="/articles/s41467-023-43718-w#ref-CR35" id="ref-link-section-d112540969e1912">35</a></sup>. The TRUPATH screening platform has been developed as an alternative to cAMP second messenger assays to minimize signal overamplification<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 35" title="Olsen, R. H. J. et al. TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat. Chem. Biol. 16, 841–849 (2020)." href="/articles/s41467-023-43718-w#ref-CR35" id="ref-link-section-d112540969e1916">35</a></sup>. Intriguingly, in contrast to U50,488 and MP1104, but similar to the mixed-action KOR agonist pentazocine, DNCP-β-NalA(<b>1</b>) exhibited KOR partial agonism at Gα<sub>i2</sub>, Gα<sub>i3</sub>, Gα<sub>oA</sub>, Gα<sub>oB</sub> and Gα<sub>gastducin</sub> subtypes with <i>E</i><sub>max</sub> values ranging from 48% to 77%, whereas it elicited full agonism at Gα<sub>i1</sub> and Gα<sub>z</sub> with <i>E</i><sub>max</sub> values of 81% and 101%, respectively (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3i, j</a>, Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">6</a>). The highest potency in the picomolar range was observed at Gα<sub>z</sub> subtype for DNCP-β-NalA(<b>1</b>), U50,488 and MP1104, while pentazocine revealed the least variation in potency and efficacy across the transducerome (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3i, j</a>, Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">11b–d</a>, Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">5</a> and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">6</a>).</p><p>Next, we determined the opioid receptor subtype selectivity profile of DNCP-β-NalA(<b>1</b>) in radioligand binding assays with membrane preparations from HEK293 cells stably expressing the mouse MOR and DOR, and CHO cells stably expressing human nociceptin (NOP) receptor. Herein, DNCP-β-NalA(<b>1</b>) bound to mouse MOR and DOR with <i>K</i><sub>i</sub> values of 5.4 and 318 nM, respectively, supporting an ~80-fold selectivity for KOR over DOR (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3f</a>) whereas it bound to the human NOP with a <i>K</i><sub>i</sub> value of ~1.3 µM, thus having an ~330-fold selectivity for KOR over NOP receptor (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">12</a>). In the functional cAMP assay, DNCP-β-NalA(<b>1</b>) was inactive at both mouse MOR and DOR up to 10 µM (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig3">3g, h</a>). This was confirmed at the human MOR and DOR in the [<sup>35</sup>S]GTPγS binding assay (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">13</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">4</a>). We next determined the mechanism of antagonism of DNCP-β-NalA(<b>1</b>) by measuring adenylyl cyclase-mediated cAMP inhibition and [<sup>35</sup>S]GTPγS binding at mouse and human MOR, respectively, using Schild regression analysis. The MOR expressed in HEK293 and CHO cells was activated by DAMGO in the absence and presence of increasing concentrations of DNCP-β-NalA(<b>1</b>). We observed a rightward shift of the concentration-response curves of DAMGO in cAMP (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">14a</a>) and [<sup>35</sup>S]GTPγS binding assays (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">14b</a>). Schild analysis of DNCP-β-NalA(<b>1</b>) exhibited linear regression slopes of 0.9 and 1.5 (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">14c, d</a>) and pA2 values of 9.1 and 7.9 in cAMP and [<sup>35</sup>S]GTPγS binding assays, respectively, which corresponds to an average functional affinity of 0.8 and 13 nM, respectively, thus demonstrating the competitive antagonism of the DNCP-β-NalA(<b>1</b>) at MOR.</p><h3 class="c-article__sub-heading" id="Sec6">Antinociceptive efficacy and lack of typical KOR-mediated side effects of designed peptide–small molecule conjugate</h3><p>To further support in vitro findings of DNCP-β-NalA(<b>1</b>), we confirmed its serum stability (>95% remaining intact after 48 h; Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">15</a>) and then examined its antinociception in vivo after subcutaneous (s.c.) administration to male mice. Antinociceptive effects were evaluated in two mouse models of pain, the formalin test and Complete Freund’s Adjuvant (CFA)-induced inflammatory hyperalgesia. In the formalin test, s.c. administration of DNCP-β-NalA(<b>1</b>) produced a dose-dependent reduction in the pain behavior of formalin-injected male mice with significant effects at doses of 1.9 and 3.8 µmol kg<sup>−1</sup> (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4a</a>). The prototypical KOR agonist U50,488 also produced a dose-dependent decrease in the nociceptive response with a significant effect at all tested doses (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4b</a>). The calculated antinociceptive <i>ED</i><sub>50</sub> values in the formalin test of DNCP-β-NalA(<b>1</b>) and U50,488 were 1.89 µmol kg<sup>−1</sup> (95% confidence limits, 95% CL: 0.97–3.66) and 1.64 µmol kg<sup>−1</sup> (95% CL: 0.77–3.53), respectively, revealing that DNCP-β-NalA(<b>1</b>) was equipotent to U50,488. To evaluate the involvement of KOR in DNCP-β-NalA(<b>1</b>)-induced antinociception, the effect of the selective KOR antagonist nor-binaltorphimine (nor-BNI) was tested. Pretreatment of male mice with nor-BNI (13.6 µmol kg<sup>−1</sup>, s.c.) significantly reversed the antinociceptive response of DNCP-β-NalA(<b>1</b>), confirming a KOR-dependent antinociceptive effect of DNCP-β-NalA(<b>1</b>) in the formalin test (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4c</a>).</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-4" data-title="In vivo pharmacology of DNCP-β-NalA(1) after s.c. administration in male mice."><figure><figcaption><b id="Fig4" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 4: In vivo pharmacology of DNCP-β-NalA(1) after s.c. administration in male mice.</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/4" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig4_HTML.png?as=webp"><img aria-describedby="Fig4" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig4_HTML.png" alt="figure 4" loading="lazy" width="685" height="849"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-4-desc"><p><b>a</b>, <b>b</b> Formalin test, dose-dependent effect; groups: saline (<i>n</i> = 8 mice), DNCP-β-NalA(<b>1</b>) (0.8 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 1.9 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 3.8 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice) and U50,488 (1.1 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 2.1 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 5.4 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice); One-way ANOVA, F<sub>(3, 22)</sub> = 19.97, <i>P</i> < 0.0001 (<b>a</b>), and F<sub>(3, 22)</sub> = 27.10, <i>P</i> < 0.0001 (<b>b</b>). <b>c</b> Formalin test, antagonism by nor-BNI; groups: saline (<i>n</i> = 8 mice), DNCP-β-NalA(<b>1</b>) (3.8 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice) and DNCP-β-NalA(<b>1</b>)+nor-BNI (3.8 µmol kg<sup>−1</sup> + 13.6 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice); One-way ANOVA, F<sub>(2, 17)</sub> = 29.67, <i>P</i> < 0.0001. <b>d</b>, <b>e</b> CFA-induced inflammatory hyperalgesia, dose- and time-dependent effect; groups: saline (<i>n</i> = 8 mice), DNCP-β-NalA (<b>1</b>) (0.4 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 0.8 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 1.9 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice) and U50,488 (0.2 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 0.6 µmol kg<sup>−1</sup>, <i>n</i> = 7 mice; 2.1 µmol kg<sup>−1</sup>, <i>n</i> = 8 mice); Two-way ANOVA, F<sub>(3,</sub> <sub>176)</sub> = 46.10, <i>P</i> < 0.0001 (<b>d</b>), and F<sub>(3, 275)</sub> = 173.0, <i>P</i> < 0.0001 (<b>e</b>). <b>f</b> CFA-induced inflammatory hyperalgesia, antagonism by nor-BNI; groups: saline (<i>n</i> = 8 mice), DNCP-β-NalA(<b>1</b>) (1.9 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice) and DNCP-β-NalA(<b>1</b>)+nor-BNI (1.9 µmol kg<sup>−1</sup>+13.6 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice); Two-way ANOVA, F<sub>(2, 136)</sub> = 91.61, <i>P</i> < 0.0001. <b>g</b>, <b>h</b> Paw thickness, dose-dependent effect; groups: saline (<i>n</i> = 8 mice), DNCP-β-NalA(<b>1</b>) (0.8 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 1.9 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 3.8 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice) and U50,488 (1.1 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 2.1 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 5.4 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice); One-way ANOVA, F<sub>(3, 22)</sub> = 5.016, <i>P</i> = 0.0084 (<b>g</b>), and F<sub>(3, 22)</sub> = 1.770, <i>P</i> = 0.1823. <b>i</b> Paw thickness, antagonism by nor-BNI; groups: saline (<i>n</i> = 8 mice), DNCP-β-NalA(<b>1</b>) (3.8 µmol kg<sup>−1</sup>) and DNCP-β-NalA(<b>1</b>)+nor-BNI (3.8 µmol kg<sup>−1</sup> + 13.6 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice); One-way ANOVA, F<sub>(2, 17)</sub> = 7.239, <i>P</i> = 0.0053. <b>j</b> Rotarod test, motor coordination; groups: saline (<i>n</i> = 5 mice), DNCP-β-NalA(<b>1</b>) (3.8 µmol kg<sup>−1</sup>, <i>n</i> = 6 mice; 7.6 µmol kg<sup>−1</sup>, <i>n</i> = 5 mice) and U50,488 (5.4 µmol kg<sup>−1</sup>, <i>n</i> = 5 mice); Two-way ANOVA, F<sub>(3, 51)</sub> = 7.992, <i>P</i> = 0.0002. One-way ANOVA with Dunnett’s (<b>a</b>, <b>b</b>, <b>g</b>, <b>h</b>) and Tukey’s post hoc test (<b>c</b>, <b>i</b>); Two-way ANOVA with Bonferroni’s post-hoc test for (<b>d</b>–<b>f</b>, <b>j</b>). *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001, drug vs. saline group; <sup>#</sup><i>P</i> < 0.05, <sup>##</sup><i>P</i> < 0.001, DNCP-β-NalA(<b>1</b>) vs. DNCP-β-NalA(<b>1</b>)+nor-BNI. All data represent means ± s.e.m. Source data are provided as a Source Data file.</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/4" data-track-dest="link:Figure4 Full size image" aria-label="Full size image figure 4" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div><p>Next, we investigated the antinociceptive efficacy of DNCP-β-NalA(<b>1</b>) after s.c. administration in male mice with CFA-induced inflammatory hyperalgesia. Male mice received CFA to the dorsal side of the right hindpaw, and hyperalgesia was evidenced by a significant reduction at 72 h post-inoculation versus pre-innoculation (<i>P</i> < 0.001, paired <i>t</i>-test) in paw withdrawal thresholds to thermal stimulation assessed with the Hargreaves test. Male mice were treated s.c. with saline, and different doses of DNCP-β-NalA(<b>1</b>), or U50,488, and tested for thermal sensitivity (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4d,</a><a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">e</a>, respectively). DNCP-β-NalA(<b>1</b>) produced time- and dose-dependent increase in the inflamed paw withdrawal latencies. Compared to saline-treated mice, DNCP-β-NalA(<b>1</b>) significantly reduced thermal sensitivity at doses of 0.8 and 1.9 µmol kg<sup>−1</sup> (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4d</a>). Notable was the fast onset of the antihyperalgesic effect of DNCP-β-NalA(<b>1</b>) with a peak effect at 15 min followed by a rapid decline, with thermal nociceptive thresholds returning to basal values at 2 h after drug administration.</p><p>Administration of U50,488 also caused a dose-dependent attenuation in pain behavior of male mice with CFA-induced inflammatory hyperalgesia (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4e</a>). Doses of 0.6 and 2.1 mg kg<sup>−1</sup> of U50,488 significantly increased paw withdrawal latencies from 15 min to 1 h, and from 15 min to 6 h, respectively, with a peak antinociceptive effect at 30 min. Although DNCP-β-NalA(<b>1</b>) had a shorter duration of the antinociceptive effect than U50,488, it showed comparable antinociceptive efficacy at the highest tested dose in attenuating the pain response in male mice with CFA-induced inflammatory hyperalgesia. We also demonstrated that the antinociceptive effect of DNCP-β-NalA(<b>1</b>) (1.9 mg kg<sup>−1</sup>) was reversed by pretreatment with the nor-BNI (13.6 µmol kg<sup>−1</sup>, s.c.), indicating that the KOR is involved in DNCP-β-NalA(<b>1</b>) in vivo agonist activity (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4f</a>). Altogether, these data show that DNCP-β-NalA(<b>1</b>) efficiently reversed thermal hyperalgesia in male mice with CFA-induced inflammatory pain acting through the KOR, with a fast onset of action.</p><p>We then explored the potential anti-inflammatory effect of DNCP-β-NalA(<b>1</b>) after s.c. administration in male mice by measuring paw thickness, 60 min after formalin injection into the right hind paw. DNCP-β-NalA(<b>1</b>) significantly reduced paw thickness of the formalin-injected paw at doses of 1.9 and 3.8 µmol kg<sup>−1</sup>, by 12 ± 2% and 17 ± 3%, respectively, compared to saline-treated mice (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4g</a>). By contrast, U50,488 did not affect paw oedema formation at any of the tested doses when compared to the saline group (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4h</a>). The DNCP-β-NalA(<b>1</b>) inhibitory effect on the formalin-induced paw inflammation was significantly reversed by pre-treatment with nor-BNI (13.6 µmol kg<sup>−1</sup>, s.c.), indicating the contribution of KOR to the anti-inflammatory effect of DNCP-β-NalA(<b>1</b>) (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4i</a>).</p><p>Finally, we investigated the behavioral effects of DNCP-β-NalA(<b>1</b>) on motor coordination and the potential to induce sedation in male mice using the rotarod test (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig4">4j</a>). Male mice were administered the highest antinociceptive effective dose in the formalin test, i.e., 3.8 µmol kg<sup>−1</sup> of DNCP-β-NalA(<b>1</b>) and 5.4 µmol kg<sup>−1</sup> of U50,488. The rotarod test was also performed in male mice receiving a dose of 7.6 µmol kg<sup>−1</sup> of DNCP-β-NalA(<b>1</b>). While U50,488 caused a significant deficit in the rotarod performance at 30 min in comparison with saline mice, DNCP-β-NalA(<b>1</b>) produced no changes in the motor behavior of male mice with no significant alterations in rotarod latencies at any time point and tested doses. These data demonstrate that DNCP-β-NalA(<b>1</b>) produces significant and potent antinociception in the formalin test and CFA-induced inflammatory hyperalgesia, along with anti-inflammatory effects, without KOR-mediated liability of motor dysfunction/sedation after s.c. administration in male mice.</p><h3 class="c-article__sub-heading" id="Sec7">Structural validation of peptide–small molecule design</h3><p>High-resolution structures are instrumental for evaluating the accuracy of computationally designed molecules. We thus determined the cryo-EM structure of human KOR bound to DNCP-β-NalA(<b>1</b>) and Gα<sub>i1</sub>/Gβ<sub>1</sub>/Gγ2 heterotrimer at the nominal resolution of 2.6 Å (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5a</a>, Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">16</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">7</a>). This high resolution enables the unambiguous modeling of KOR and G protein heterotrimer (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">16</a> and Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">17</a>). The overall structure of the KOR-G<sub>i1</sub> protein heterotrimer is similar to the MOR-G<sub>i1</sub> structure<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 36" title="Koehl, A. et al. Structure of the micro-opioid receptor-Gi protein complex. Nature 558, 547–552 (2018)." href="/articles/s41467-023-43718-w#ref-CR36" id="ref-link-section-d112540969e2626">36</a></sup>, likely due to stabilization by the same G protein subtype (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">18a, b</a>). The major difference is in the Gα helix where the N-terminal α-helix (αN) in KOR-G<sub>i1</sub> has a 3 Å displacement and α<sub>1</sub> has a 2 Å displacement compared to that in MOR-G<sub>i1</sub> structure. KOR adopts an active-state conformation with the hallmark of 10 Å outward movement of transmembrane helix 6 (TM6) compared to the inactive-state KOR<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 37" title="Wu, H. et al. Structure of the human kappa-opioid receptor in complex with JDTic. Nature 485, 327–332 (2012)." href="/articles/s41467-023-43718-w#ref-CR37" id="ref-link-section-d112540969e2640">37</a></sup> (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">18c</a>).</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-5" data-title="Cryo-EM structure of the KOR-DNCP-β-NalA(1)-Gi1 complex."><figure><figcaption><b id="Fig5" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 5: Cryo-EM structure of the KOR-DNCP-β-NalA(1)-G<sub>i1</sub> complex.</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/5" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig5_HTML.png?as=webp"><img aria-describedby="Fig5" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_Fig5_HTML.png" alt="figure 5" loading="lazy" width="685" height="1164"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-5-desc"><p><b>a</b> Overall architecture of the active state human KOR bound to DNCP-β-NalA(<b>1</b>) and G-protein heterotrimer (Gα<sub>i1</sub>, Gβ<sub>1</sub>, Gγ<sub>2</sub>). The KOR-G-protein complex was further stabilized by a single-chain antibody scFv16. The right panel shows the binding pose of DNCP-β-NalA(<b>1</b>) at KOR. The highly conserved anchoring residue D138, as part of the orthosteric binding pocket of KOR is shown. <b>b</b> The interactions between the bottom half of DNCP-β-NalA(<b>1</b>) and the orthosteric site of KOR. <b>c</b> Effects of orthosteric residues on DNCP-β-NalA(<b>1</b>)-mediated Gα<sub>i1</sub> protein and β-arrestin-2 signaling (<i>n</i> = 3). For KOR D138N mutant, the reference ligand is salvinorin A because U50,488 is inactive at this mutant. <b>d</b> The interactions between the peptide macrocycle of DNCP-β-NalA(<b>1</b>) and the extracellular binding pocket 2 of KOR. <b>e</b> Effects of binding pocket 2 residues on DNCP-β-NalA(<b>1</b>)-mediated Gα<sub>i1</sub> protein and β-arrestin-2 signaling (<i>n</i> = 3). All data are presented as mean values ± s.e.m. Source data are provided as a Source Data file.</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/articles/s41467-023-43718-w/figures/5" data-track-dest="link:Figure5 Full size image" aria-label="Full size image figure 5" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div><p>DNCP-β-NalA(<b>1</b>) occupies two major binding pockets of KOR. One is the orthosteric site by the small molecule portion (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5b</a>), which is also the canonical binding site for “classical” KOR ligands; the other pocket is in the extracellular region formed by ECL2, ECL3, TM6, and TM7 (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5c</a>), which is occupied by the peptide moiety. The small molecule portion of DNCP-β-NalA(<b>1</b>) (bottom half) adopts a conformation similar to MP1104, as well as other typical KOR agonists including U50,488 and pentazocine (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">19</a>). Comparison of the computationally designed model DNCP-β-NalA(<b>1</b>) with the experimental structure shows a conserved binding mode of the D-Phe at position 1. The initial goal of the design was to form interactions with both the ECL2 and ECL3. We are still seeing these interactions but in an altered manner: in the designed model the peptide was predicted to have a D-Tyr (DNCP1_design D-Tyr–R<sub>3</sub>) interaction with ECL2 and a Tyr (DNCP1_design Tyr–R<sub>1</sub>) interaction with ECL3. We found that the residue D-Tyr (R<sub>3</sub>) interacted with the ECL3 versus the predicted interaction with ECL2 (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">20</a>). Further structural information of the other computationally designed peptides whose ECL2/3 interactions are similar to those of computational DNCP-β-NalA(<b>1</b>) (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">21</a>) would elucidate the peptides’ ability to induce an altered conformation upon binding. To explore the stability and molecular interactions of the peptide component in the extracellular pocket, we conducted a total of four individual molecular dynamics (MD) simulations, each lasting 500 ns. The simulations’ reliability was assessed by analyzing the root mean square deviation of protein backbone atoms (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">22</a>) indicating consistent stability throughout. Comparisons of the calculated average distances between Cα-atoms of the receptor and Cα-atoms of the peptide moiety of DNCP-β-NalA(<b>1</b>) from the MD simulations and experimentally derived data from the cryo-EM structure indicate that the peptide portion of the ligand maintained similar interactions pattern to the experimentally determined structure (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">8</a> and Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">23</a>). To assess the amino acid residues that are involved in ligand binding throughout the MD simulations, we calculated the fraction of protein amino acid residues within a 4.5 Å distance from the bound ligand in the combined simulations trajectories (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">24</a>). Within binding pocket 1, the ligand demonstrated molecular interactions with Q115<sup>2.60</sup>, L135<sup>3.29</sup>, D138<sup>3.32</sup>, Y139<sup>3.33</sup>, M142<sup>3.36</sup>, V230<sup>5.42</sup>, W287<sup>6.48</sup>, I290<sup>6.51</sup>, I316<sup>7.39</sup>, G319<sup>7.42</sup>, and Y320<sup>7.43</sup>, which align well with the interactions observed in the previously published structure (<a href="https://doi.org/10.2210/pdb6B73/pdb">PDB: 6B73</a>). In peptide-ring binding pocket 2, the ligand primarily engaged in molecular interactions with E209<sup>ECL2</sup>, C210<sup>ECL2</sup>, L212<sup>ECL2</sup> and Y313<sup>7.36</sup>, as well as amino acid residues S303<sup>ECL3</sup>, H304<sup>ECL3</sup>, A308<sup>7.31</sup>, and L309<sup>7.32</sup> in strong agreement with the cryo-EM data. The peptide portion of the ligand also had significant contact frequency with amino acid residues located on TM6 and TM7: F293<sup>6.54</sup>, I294<sup>6.55</sup>, E297<sup>6.58</sup> and Y312<sup>7.35</sup> (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">24</a>).</p><p>The aspartic acid residue D138<sup>3.32</sup> has been known as an anchoring residue important for the binding and signaling of many KOR agonists that usually forms hydrogen-bond (H-bond) interactions with the ligand<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 37" title="Wu, H. et al. Structure of the human kappa-opioid receptor in complex with JDTic. Nature 485, 327–332 (2012)." href="/articles/s41467-023-43718-w#ref-CR37" id="ref-link-section-d112540969e2840">37</a></sup>. It is interesting that D138<sup>3.32</sup> appears to form a weak salt-bridge interaction (3.8 Å) with DNCP-β-NalA(<b>1</b>) and the D138<sup>3.32</sup>N mutation caused an 8-fold loss of potency for DNCP-β-NalA(<b>1</b>), but a 1,000-fold for U50,488 (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5a</a>, Supplementary Figs. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">25</a>, <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">26</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">9</a>). This is further confirmed by the reduced binding affinity of DNCP-β-NalA(<b>1</b>) in KOR D138A or D138N mutants (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">27</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">10</a>). This is in accordance with the MD simulations where the distance between the ligand and D138<sup>3.32</sup> sampled a wide distance distribution (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">28</a>). This could be due to the massive interactions formed between KOR and other parts of DNCP-β-NalA(<b>1</b>), as the mutational screening showed that residues from both binding pockets could dramatically affect the potency and/or efficacy of DNCP-β-NalA(<b>1</b>) in G protein and β-arrestin activation (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5b–e</a>, Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">11</a> and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">12</a>). In binding pocket 1 (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5b</a>), while most of the alanine mutations decreased the agonist activity of DNCP-β-NalA(<b>1</b>), the Q115<sup>2.60</sup>A had only a minimal effect on G<sub>i1</sub> activation, but significantly increased the efficacy of β-arrestin-2 recruitment (60% vs. 35% in WT) (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5c</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">11</a>). The I135<sup>3.29</sup>A and K227<sup>5.39</sup>A appear to specifically affect DNCP-β-NalA(<b>1</b>)’s efficacy in receptor activation (G<sub>i1</sub> activation, KOR-WT 86%, I135<sup>3.29</sup>A 46%, K227<sup>5.39</sup>A 48%), while not or slightly reduces the potency (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5c</a> and Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">11</a>). The more profound observation was from the mutational analysis of residues in the second binding pocket (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5d</a>) that may have formed H-bond or hydrophobic interactions (residues within 4 Å of the ligand) with the peptide ring. Several residue mutations, such as E209<sup>ECL2</sup>A, E297<sup>6.58</sup>A and L309<sup>7.32</sup>A led to increased potency in G protein activation and enhanced efficacy in arrestin recruitment (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">29</a>, Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">12–14</a>). The role of extracellular vestibule has been investigated in the KOR-dynorphin structure<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 38" title="Wang, Y. et al. Structures of the entire human opioid receptor family. Cell 186, 413–427, (2023)." href="/articles/s41467-023-43718-w#ref-CR38" id="ref-link-section-d112540969e2951">38</a></sup>, in which mutations of ECL2/3 led to significant reduction of dynorphin A<sub>1-13</sub>’s agonist activity. Comparison of dynorphin with DNCP-β-NalA(<b>1</b>) shows overlapping of several binding sites (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">29</a>). Characterization of dynorphin in KOR E209<sup>ECL2</sup>A, E297<sup>6.58</sup>A or L309<sup>7.32</sup>A mutants displayed opposite effects compared to DNCP-β-NalA(<b>1</b>), suggesting that the ECL2/3 and extracellular transmembrane ends play important roles in regulating ligand-specific responses during opioid receptor activation (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">29</a>, Supplementary Tables <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">13</a> and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">14</a>). The MD simulations indicate potential interactions between the ligand and amino acid residues (E209<sup>ECL2</sup>, E297<sup>6.52</sup> and L309<sup>7.32</sup>). In most simulations, the highest probability of distance consistently remained below 6 Å. However, in certain simulations, larger distance probability values were observed, indicating increased flexibility between the ligand and those specific residues (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">28</a>). Another residue, Y312<sup>7.35</sup> (TM7) has been reported to affect KOR’s functional selectivity by affecting arrestin signaling<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e2995">8</a></sup>. This amino acid residue is involved in hydrophobic contacts with the ligand more than 95% of the simulation trajectory (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">24</a>). The Y312<sup>7.35</sup>A mutant activated by DNCP-β-NalA(<b>1</b>) was devoid of arrestin recruitment while only a 4-fold loss of potency in G protein activation (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41467-023-43718-w#Fig5">5e</a>) could be observed. It is important to note that the cell surface expression levels of all mutants were within 2-fold range compared to the wild-type KOR as determined by ELISA (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">30</a>).</p></div></div></section><section data-title="Discussion"><div class="c-article-section" id="Sec8-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec8">Discussion</h2><div class="c-article-section__content" id="Sec8-content"><p>Our designed thioether cyclized peptides conjugated to β-NalA—a small molecule, partial agonist of the KOR whose structure resembles MP1104<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 26" title="Varadi, A. et al. Synthesis and characterization of a dual kappa-delta opioid receptor agonist analgesic blocking cocaine reward behavior. ACS Chem. Neurosci. 6, 1813–1824 (2015)." href="/articles/s41467-023-43718-w#ref-CR26" id="ref-link-section-d112540969e3023">26</a></sup>—mimic the orthosteric properties of MP1104<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e3027">8</a></sup> and simultaneously interact with secondary binding sites on KOR. In contrast to high-throughput screening methods and recent examples of (ultra) large library docking of compounds<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 39" title="Fink, E. A. et al. Structure-based discovery of nonopioid analgesics acting through the alpha(2A)-adrenergic receptor. Science 377, eabn7065 (2022)." href="/articles/s41467-023-43718-w#ref-CR39" id="ref-link-section-d112540969e3031">39</a></sup>, we overcome multiple rounds of structure-based design and pharmacological testing; we need to synthesize and experimentally characterize only four compounds to discover a high-affinity molecule with patterns of GPCR signaling and pharmacology. This compound, DNCP-β-NalA(<b>1</b>), is a full agonist, exhibiting nanomolar KOR potency, with preferential efficacy bias for G proteins over β-arrestins—this is notable as KOR agonists with partial β-arrestin recruitment are often associated with fewer side effects in vivo<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 31" title="Brust, T. F. et al. Biased agonists of the kappa opioid receptor suppress pain and itch without causing sedation or dysphoria. Sci. Signal. 9, aai8441 (2016)." href="/articles/s41467-023-43718-w#ref-CR31" id="ref-link-section-d112540969e3038">31</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 40" title="Bedini, A. et al. Functional selectivity and antinociceptive effects of a novel KOPr agonist. Front. Pharmacol. 11, 188 (2020)." href="/articles/s41467-023-43718-w#ref-CR40" id="ref-link-section-d112540969e3041">40</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 41" title="Spetea, M. et al. Selective kappa receptor partial agonist HS666 produces potent antinociception without inducing aversion after i.c.v. administration in mice. Br. J. Pharmacol. 174, 2444–2456 (2017)." href="/articles/s41467-023-43718-w#ref-CR41" id="ref-link-section-d112540969e3044">41</a></sup>. Subtype selectivity on the morphinan template is difficult to achieve (MP1104 is a pan opioid binder)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 42" title="Spetea, M. & Schmidhammer, H. Recent chemical and pharmacological developments on 14-oxygenated-N-methylmorphinan-6-ones. Molecules 26, 
 https://doi.org/10.3390/molecules26185677
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR42" id="ref-link-section-d112540969e3049">42</a></sup>; DNCP-β-NalA(<b>1</b>) exhibits ~80-and ~330-fold selectivity for KOR over DOR, and for KOR over NOP receptor, respectively, but is equipotent at MOR. The mixed action of DNCP-β-NalA(<b>1</b>) as biased agonist at KOR and competitive antagonist at MOR might thus contribute to the observed antinociceptive efficacy and result in a favorable side effect profile in male mice. The development of mixed KOR agonists/MOR antagonists has been explored as a strategy to develop safer pain medications<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 12" title="Dalefield, M. L., Scouller, B., Bibi, R. & Kivell, B. M. The kappa opioid receptor: a promising therapeutic target for multiple pathologies. Front. Pharmacol. 13, 837671 (2022)." href="/articles/s41467-023-43718-w#ref-CR12" id="ref-link-section-d112540969e3059">12</a></sup>.</p><p>The cryo-EM structure of DNCP-β-NalA(<b>1</b>) bound to KOR revealed a molecular network of residues involving ECL2/3 and TM6/7 that control the functional selectivity of the receptor. The mutations E209<sup>ECL2</sup>A, E297<sup>6.58</sup>A and L309<sup>7.32</sup>A increase β-arrestin efficacy bias and G<sub>i</sub> potency of DNCP-β-NalA(<b>1</b>). The TM5-ECL2 region has previously been proposed to modulate bias at other GPCRs such as opioid<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 29" title="Uprety, R. et al. Controlling opioid receptor functional selectivity by targeting distinct subpockets of the orthosteric site. Elife 10, 
 https://doi.org/10.7554/eLife.56519
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR29" id="ref-link-section-d112540969e3081">29</a></sup>, serotonin<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 43" title="McCorvy, J. D. et al. Structure-inspired design of beta-arrestin-biased ligands for aminergic GPCRs. Nat. Chem. Biol. 14, 126–134 (2018)." href="/articles/s41467-023-43718-w#ref-CR43" id="ref-link-section-d112540969e3085">43</a></sup> and dopamine<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 44" title="Chun, L. S. et al. Structure-activity investigation of a G protein-biased agonist reveals molecular determinants for biased signaling of the D2 dopamine receptor. Front. Synaptic Neurosci. 10, 2 (2018)." href="/articles/s41467-023-43718-w#ref-CR44" id="ref-link-section-d112540969e3089">44</a></sup> receptors. Conversely, Y312<sup>7.35</sup>A, as previously determined for KOR and MOR<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 29" title="Uprety, R. et al. Controlling opioid receptor functional selectivity by targeting distinct subpockets of the orthosteric site. Elife 10, 
 https://doi.org/10.7554/eLife.56519
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR29" id="ref-link-section-d112540969e3095">29</a></sup>, shifted bias towards the G<sub>i</sub> protein and reduced arrestin recruitment. Both U50,488 and MP1104 were previously identified as balanced KOR agonists with ~100% efficacy in the β-arrestin recruitment<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e3102">8</a></sup>. While the small molecule portion of DNCP-β-NalA(<b>1</b>) adopts a similar conformation to U50,488 and MP1104, the peptide ring forms extensive interactions with ECL2/3 and TM6/7 residues, suggesting that these additional interactions may account for DNCP-β-NalA(<b>1</b>)’s G protein-biased or partial agonist activity.</p><p>A major challenge in developing functionally specific molecules is the incorporation of multiple receptor conformational states in the design process<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 45" title="Yang, D. et al. G protein-coupled receptors: structure- and function-based drug discovery. Signal. Transduct. Target Ther. 6, 7 (2021)." href="/articles/s41467-023-43718-w#ref-CR45" id="ref-link-section-d112540969e3115">45</a></sup>. The high-resolution cryo-EM structure revealed conformational changes in both the ECL2/3 and the designed peptide. Here, we only utilize the known experimental structure of KOR, which biased the designs toward that conformation; the design protocol can likely be improved by sampling alternative states of the flexible ECLs and peptide using molecular dynamics simulation or other approaches, or by utilizing predicted conformational states of the full receptor. Further improvement of the computational methods and more experimental data should enable design of more selective agonists in the future.</p><p>Our design approach coupling a small molecule anchor known to bind KOR, to the free terminus of a lariat-shaped peptide with a cyclic peptide portion making interactions with other portions of the receptor outside the orthosteric site should be broadly applicable to other GPCRs. As illustrated here, the small molecule anchor provides some starting affinity and specificity, which can then be increased and modulated by the interaction of the cyclic peptide portion with the receptor. DNCP-β-NalA(<b>1</b>) displays partial β-arrestin recruitment and selectivity for KOR over DOR and NOP receptor, together with KOR-mediated antinociceptive efficacy and anti-inflammatory effects and reduced risk for KOR-related liabilities in male mice. Overall, our approach provides a route to generating diverse sets of high affinity and selective ligands for disentangling the pharmacology of GPCRs and addressing outstanding therapeutic challenges.</p></div></div></section><section data-title="Methods"><div class="c-article-section" id="Sec9-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec9">Methods</h2><div class="c-article-section__content" id="Sec9-content"><h3 class="c-article__sub-heading" id="Sec10">Ethical statement</h3><p>Experiments were performed in male SWISS mice (RjOrl:SWISS; 8–10 weeks old, 30–35 g body weight) purchased from Janvier Labs (Le Genest-Saint-Isle, France). All animal care and experimental procedures were in accordance with the ethical guidelines for the animal welfare standards of the European Communities Council Directive (2010/63/EU) and were approved by the Committee of Animal Care of the Austrian Federal Ministry of Science and Research (Protocol Nr. 2022-0.159.599).</p><h3 class="c-article__sub-heading" id="Sec11">Computational design</h3><p>For protein preparation and ligand parametrization the crystal structure of human KOR was obtained from the Protein Data Bank (<a href="https://doi.org/10.2210/pdb6B73/pdb">PDB: 6B73</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e3152">8</a></sup>. KOR was in complex with the epoxymorphinan opioid agonist MP1104 and an active-state-stabilizing nanobody. The nanobody, cholesterol and oleic acid were removed prior to preparation of the structure. To allow MP1104 modeling in Rosetta, we built the necessary ligand parameter file (Supplementary Data <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM7">4</a>). MP1104 was modified by removing the iodobenzamide moiety (yielding N-cyclopropylmethyl-epoxy morphinan), leaving behind a free amine (which we refer as CVV, and which corresponds to β-NalA), which enables conjugation to a carboxylic acid. Open Babel<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 46" title="O’Boyle, N. M. et al. Open Babel: an open chemical toolbox. J. Cheminform. 3, 33 (2011)." href="/articles/s41467-023-43718-w#ref-CR46" id="ref-link-section-d112540969e3159">46</a></sup> was used to generate the necessary input conformers. The mol2 structure of CVV was converted to Rosetta’s params file format using the molfile_to_params.py script included with the software. Based on the binding pocket analysis, sampling of hexameric thioether macrocycles was focused on. Backbones were generated through a custom sampling script (Supplementary Data <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM4">1</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 47" title="Coutsias, E. A., Seok, C., Jacobson, M. P. & Dill, K. A. A kinematic view of loop closure. J. Comput. Chem. 25, 510–528 (2004)." href="/articles/s41467-023-43718-w#ref-CR47" id="ref-link-section-d112540969e3167">47</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 48" title="Mandell, D. J., Coutsias, E. A. & Kortemme, T. Sub-angstrom accuracy in protein loop reconstruction by tics-inspired conformational sampling. Nat. Methods 6, 551–552 (2009)." href="/articles/s41467-023-43718-w#ref-CR48" id="ref-link-section-d112540969e3170">48</a></sup>. The backbones were clustered using a torsion bin-based clustering method<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 21" title="Hosseinzadeh, P. et al. Comprehensive computational design of ordered peptide macrocycles. Science 358, 1461–1466 (2017)." href="/articles/s41467-023-43718-w#ref-CR21" id="ref-link-section-d112540969e3174">21</a></sup>. Prior to docking the cyclic peptide backbones, a two amino acids extension from the free amine of the small molecule anchor (also known as stub) CVV was sampled. The extension was added using PeptideStubMover. All 20 potential amino acids (both D-/L-) were tested as the extension, minimized, and scored. The results were analyzed using python pandas and residues and orientations that had the best interface metrics (high shape complementarity and low ΔΔG) were selected for the extension. The docking was performed using a python script that simply transforms the C,N,O,CA, and CB atoms of the acetylated C-terminal amino acid CYS (known as the 3-letter code: CYY) of each backbone peptide residue onto the corresponding atoms in temporary extended and randomly sampled CYY residue of the anchor (Supplementary Data <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM5">2</a>). The docked conformations were then relaxed using Rosetta FastRelax<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 49" title="Tyka, M. D. et al. Alternate states of proteins revealed by detailed energy landscape mapping. J. Mol. Biol. 405, 607–618 (2011)." href="/articles/s41467-023-43718-w#ref-CR49" id="ref-link-section-d112540969e3181">49</a></sup> and those with minimal clashes were further carried out for design. Peptide sequences were subsequently designed using Rosetta’s FastDesign mover<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 18" title="Bhardwaj, G. et al. Accurate de novo design of hyperstable constrained peptides. Nature 538, 329–335 (2016)." href="/articles/s41467-023-43718-w#ref-CR18" id="ref-link-section-d112540969e3185">18</a></sup>. The details of the design script <kappa_peptide_design.xml> are provided (Supplementary Data <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM6">3</a>). The designed peptides were then filtered based on their total Rosetta score, contact molecular surface, shape complementarity, ΔΔG of binding, and contacts at interface using python pandas (Supplementary Fig. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">2</a>). The designs in the top 10% of all these metrics were chosen for further analysis. Selected designed models were then visually inspected and those with minimum number of buried unsatisfied hydrogen bond donors and acceptors were selected for synthesis.</p><h3 class="c-article__sub-heading" id="Sec12">Peptide synthesis</h3><p>Peptides were synthesized either manually or on an automatic Liberty Blue™ microwave peptide synthesizer (CEM Corporation, NC, USA) (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">1</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 50" title="Cheneval, O. et al. Fmoc-based synthesis of disulfide-rich cyclic peptides. J. Org. Chem. 79, 5538–5544 (2014)." href="/articles/s41467-023-43718-w#ref-CR50" id="ref-link-section-d112540969e3207">50</a></sup>. DNLPs (<b>11</b>–<b>16</b>) and DNCPs (<b>21</b>–<b>26</b>) without small molecule were synthesized as C-terminal amides using Rink amide resin. For DNCPs (31–36) to be used for conjugation a D-Phe preloaded Wang resin (0.7 mmol g<sup>−1</sup>) was used. After peptide assembly, DNLPs (<b>11</b>–<b>16</b>) were cleaved from the resin by treatment with Cleavage Cocktail B [trifluoroacetic acid (88% v/v), phenol (5% v/v), water (5% v/v), triisopropylsilane (2% v/v)] for 1 h, followed by diethlyether precipitation. To produce the DNCPs, the peptides were subjected cyclized on the resin after assembly using Fmoc-SPPS. First, the N-terminus was bromoacetylated (10 eq. of bromoacetic acid treated with 5 eq. of dicyclohexylcarbodiimide for 1 h), followed by on-resin cleavage of the methoxytrityl group of cysteine (with 2% trifluoroacetic acid in dichloromethane, 12 × 5 min) and finally cyclized to form a thioether (1% N,N-diisopropylethylamine in N,N-dimethylformamide, 18 h). These DNCPs (<b>21</b>–<b>26</b> and <b>31</b>–<b>36</b>) were then cleaved from resin by treatment with cleavage cocktail B for 1 h, followed by diethylether precipitation. DNCPs with a C-terminal COOH (<b>31</b>–<b>36</b>) were coupled to the free NH<sub>2</sub> of β-NalA by dissolving the peptide (1 eq.) in dimethylformamide containing HATU (1 eq., 0.4 M) and adding β-NalA (1.1 eq., 25 mM) and diisopropylethylamine (2 eq.) at 0 °C. Reaction mixture was allowed to warm to room temperature and stirred for 30 min. [<i>Note:</i> above conc. of stock solutions (0.4 M and 25 mM) were necessary, as at a higher dilution, the conjugation reaction (for DNCP <b>31</b>) was incomplete, even after 24 h]. Purification of all peptides was carried out either by RP-HPLC instrument from Waters P150 system with 2545 quaternary gradient module, a Waters 2489 UV/Vis detector and a waters fraction collector III (Waters, MS, USA) or a LC-20AT system equipped with an SPD-20A Prominence UV/VIS detector, and FRC-10A fraction collector using preparative or semipreparative Phenomenex Jupiter C<sub>18</sub> columns (5 μm, 300 Å, 250 × 21.2 mm or 250 × 10 mm) (Phenomenex, Aschaffenburg, Germany) or a Kromasil C<sub>18</sub> column (10 μm, 300 Å, 250 × 21.2 mm or 250 × 10 mm) (AkzoNobel, Göteborg, Sweden). Linear gradients from 5% to 65% solvent B (90% acetonitrile, 10% H<sub>2</sub>O, 0.05% trifluoroacetic acid) and flow rates of 8 mL min<sup>−1</sup> or 20 mL min<sup>−1</sup> and 3 mL min<sup>−1</sup> or 8 mL min<sup>−1</sup> were applied for preparative and semipreparative RP-HPLC, respectively. Fractions were collected automatically. Quality of fractions and purified peptides were analyzed by electrospray ionization mass spectrometry (ESI-MS) and analytical RP-HPLC and RP-UPLC (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">2</a>, Supplementary Figs. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">4</a>, <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">5</a>, <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">7</a>, and <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">8</a>). For RP-HPLC and RP-UPLC characterization of peptides, a Phenomenex Jupiter C<sub>18</sub> column (5 μm, 300 Å, 150 × 2 mm) and Phenomenex Luna Omega C<sub>18</sub> column (1.6 µm 100 Å, 50 × 2.1 mm) were used, respectively. Desired peptide mass was identified by ESI-MS either on LCMS-2020 (Shimadzu, Kyoto, Japan) coupled to an amaZon-SL ion trap or a Brucker Daltonics QqTOF compact (Billerica, MA, USA). For the latter analysis on the QqTOF system a PepMap Acclaim capillary column (Thermo Fisher Scientific; 150 × 0.3 mm, 2 μm, 100 Å) at flow rates of 25 μL min<sup>-1</sup> for the loading pump and 6 μL min<sup>−1</sup> for the separation pump were used with linear gradients of solvent D (0.08% formic acid in acetonitrile) and solvent C (0.1% formic acid in water).</p><h3 class="c-article__sub-heading" id="Sec13">Peptide content determination</h3><p>Peptide concentrations were determined based on peak area detected at 214 nm by analytical RP-HPLC (Phenomenex-Prodigy 100 Å, 5 um, 4.6 × 50 mm, 2 mL min<sup>−1</sup>, gradient 0<b>–</b>90% B in 10 min) against two peptide standards (oxytocin and vasopressin) with known peptide content established by amino acid analysis. Using the Beer–Lambert law, the peptide concentrations were calculated based on absorbance of standards and samples using calculated extinction coefficients.</p><h3 class="c-article__sub-heading" id="Sec14">Chemical and reagents</h3><p>Radioligands [<sup>3</sup>H]diprenorphine (34.6 Ci mmol<sup>−1</sup>), [<sup>3</sup>H]nociceptin (119.4 Ci mmol<sup>−1</sup>) and guanosine 5′-O-(3-[<sup>35</sup>S]thio)-triphosphate ([<sup>35</sup>S]GTPγS, 1,250 Ci mmol<sup>−1</sup>) were purchased from PerkinElmer (Boston, MA, USA). Guanosine diphosphate (GDP), GTPγS, U50,488, U69,593, DAMGO, DPDPE, DADLE, nor-BNI, nociceptin, CFA, polyethylenimine (PEI), tris(hydroxymethyl) aminomethane (Tris), 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), formalin, forskolin, naloxone, bovine serum albumin (BSA), and cell culture media and supplements were obtained from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Opti-MEM was obtained from Gibco-ThermoFisher (Vienna, Austria). Furimazine was obtained from Promega (Madison, USA). Dyn A<sub>1–13</sub> amide trifluoroacetate salt was purchased from Bachem (Bubendorf, Switzerland). All other chemicals were of analytical grade and obtained from standard commercial sources. DNLPs, DNCPs, DNCP-β-NalA conjugates and β-NalA were solved in water, and further diluted to working concentrations in the appropriate buffer. The cAMP G<sub>i</sub> kit was from CisBio-PerkinElmer (Codolet, France) and jetPRIME transfection reagent from Polyplus (Illkirch, France).</p><h3 class="c-article__sub-heading" id="Sec15">Animals and drug administration</h3><p>Experiments were performed in male SWISS mice (RjOrl:SWISS; 8–10 weeks old, 30–35 g body weight) purchased from Janvier Labs (Le Genest-Saint-Isle, France). Here, we used male mice to establish pain and behavioral responses as these types of studies are commonly reported using either male or female mice, or both sexes. A total of 124 male SWISS mice were used in the in vivo study. Male mice were group-housed (maximum five animals per cage) in a temperature- (22–23 °C) and humidity-controlled (60–70%) specific pathogen free room with a 12 h light/dark cycle and with free access to food and water. All behavioral experiments were performed during the light cycle. Efforts were made to minimize animal suffering and to reduce the number of mice use. Animals were assigned to groups randomly, and drug treatment experiments were conducted in a blinded fashion. Experimental drugs, DNCP-β-NalA(<b>1</b>), U50,488 and nor-BNI, were prepared in sterile physiological 0.9% saline solution. Test compounds or vehicle (saline) were s.c. administered in a volume of 10 µL g<sup>−1</sup> body weight.</p><h3 class="c-article__sub-heading" id="Sec16">Cell transfection and cloning</h3><p>Cell transfection was performed with HEK293T (ATCC, CRL-3216) or CHO-K1 (ATCC, CCL-61) cells and jetPRIME transfection reagent according to the manufacturer’s protocol. For BRET assay, 1.8 µg of plasmid DNA transiently expressing the mouse KOR tagged fused to EGFP and 0.2 µg of the human β-arrestin-2 coupled to nano luciferase were used. Cloning and generation of stable HEK293T mouse KOR cell line was performed using established molecular and cell biology procedures<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 51" title="Koehbach, J. et al. Oxytocic plant cyclotides as templates for peptide G protein-coupled receptor ligand design. Proc. Natl Acad. Sci. USA 110, 21183–21188 (2013)." href="/articles/s41467-023-43718-w#ref-CR51" id="ref-link-section-d112540969e3361">51</a></sup>.</p><h3 class="c-article__sub-heading" id="Sec17">Cell culture and cell membrane preparation</h3><p>HEK293T mouse KOR, DOR, and MOR stable cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 50 U mL<sup>−1</sup> penicillin/streptomycin and 0.8 mg mL<sup>−1</sup> geneticin (G418) and grown at 37 °C and 5% CO<sub>2</sub>. CHO-K1 cells stably expressing the human MOR or DOR (CHO-hMOR or -hDOR, respectively) were grown at 37 °C in DMEM/Ham’s F12 culture medium supplemented with 10% FBS, 0.1% penicillin/streptomycin, 2 mM L-glutamine and 0.4 mg mL<sup>−1</sup> geneticin (G418). CHO-hKOR and CHO-hNOP cells were grown at 37 °C in DMEM culture medium supplemented with 10% FBS, 0.1% penicillin/streptomycin, 2 mM L-glutamine and 0.4 mg mL<sup>−1</sup> geneticin (G418). All cell cultures were maintained in a humidified atmosphere of 95% air and 5% CO<sub>2</sub>. Membranes from receptor expressing cells were prepared the following way<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 52" title="Dumitrascuta, M. et al. Antinociceptive efficacy of the micro-opioid/nociceptin peptide-based Hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking. Molecules 26, 
 https://doi.org/10.3390/molecules26113267
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR52" id="ref-link-section-d112540969e3386">52</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 53" title="Erli, F. et al. Highly potent and selective new diphenethylamines interacting with the kappa-opioid receptor: synthesis, pharmacology, and structure-activity relationships. J. Med. Chem. 60, 7579–7590 (2017)." href="/articles/s41467-023-43718-w#ref-CR53" id="ref-link-section-d112540969e3389">53</a></sup>: Exemplarily, HEK-mKOR and CHO-hKOR grown at confluence were removed from the culture plates by scraping, homogenized in 50 mM Tris-HCl buffer (pH 7.7) using a Dounce glass homogenizer, then centrifuged once and washed by an additional centrifugation at 20,000 (HEK-mKOR) or 27,000 × <i>g</i> (CHO-hKOR) for 15 min at 4 °C. Protein content of cell membrane preparations was determined by the method of Bradford using BSA as the standard<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 54" title="Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)." href="/articles/s41467-023-43718-w#ref-CR54" id="ref-link-section-d112540969e3396">54</a></sup>. All other membranes were prepared accordingly.</p><h3 class="c-article__sub-heading" id="Sec18">In vitro pharmacological assays</h3><p>Radioligand binding, cAMP and β-arrestin recruitment assays were performed according to the following protocols<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 52" title="Dumitrascuta, M. et al. Antinociceptive efficacy of the micro-opioid/nociceptin peptide-based Hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking. Molecules 26, 
 https://doi.org/10.3390/molecules26113267
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR52" id="ref-link-section-d112540969e3408">52</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 55" title="Duerrauer, L. et al. I8-arachnotocin-an arthropod-derived G protein-biased ligand of the human vasopressin V2 receptor. Sci. Rep. 9, 19295 (2019)." href="/articles/s41467-023-43718-w#ref-CR55" id="ref-link-section-d112540969e3411">55</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 56" title="Nasrollahi-Shirazi, S. et al. Functional impact of the G279S substitution in the adenosine A(1)-Receptor (A(1)R-G279S(7.44)), a mutation associated with Parkinson’s disease. Mol. Pharmacol. 98, 250–266 (2020)." href="/articles/s41467-023-43718-w#ref-CR56" id="ref-link-section-d112540969e3414">56</a></sup>: All reagents and composition of the standard binding buffer were used according to the published protocol<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 57" title="Nasrollahi-Shirazi, S. et al. Functional impact of the G279S substitution in the adenosine A1-Receptor (A1R-G279S(7.44)), a mutation associated with Parkinson’s disease. Mol. Pharmacol. 98, 250–266 (2020)." href="/articles/s41467-023-43718-w#ref-CR57" id="ref-link-section-d112540969e3418">57</a></sup>. Briefly, displacement radioligand binding assay was performed in a final volume of 300 µL containing [<sup>3</sup>H]diprenorphine ([<sup>3</sup>H]DPN), standard binding buffer, competing ligands and membranes from HEK293T cells stably expressing the mouse KOR (7–10 µg/assay), MOR (50–100 µg/assay) or DOR (25 µg/assay). Naloxone (10 µM) was used to determine non-specific binding. Radioligand, naloxone and competing ligands were prepared 4X in the binding buffer. Reaction mixture was incubated at 37 °C for 1 h followed by filtration onto 0.1% polyethlyenimine-soaked GF/C glass fiber filter (Sartorius Stedim, Göttingen, Germany) using a Skatron cell harvester (Skatron AS, Lier, Norway). The radioactivity was measured by liquid scintillation. Radioligand binding assay to the NOP receptor was performed in a final volume of 1 mL in assay buffer (50 mM Tris-HCl buffer pH 7.4 with 1 mg mL<sup>−1</sup> BSA) containing [<sup>3</sup>H]nociceptin (0.1 nM), different concentration of test ligand and membranes from CHO-K1 cells stably expressing the human NOP receptor (15–20 µg/assay). Non-specific binding was determined in the presence of 10 µM nociceptin. Samples were incubated at 25 °C for 1 h followed by filtration onto 0.5% PEI-soaked GF/C glass fiber filter. Filters were washed three times with 5 mL of ice-cold 50 mM Tris-HCl buffer (pH 7.4) using a Brandel M24R cell harvester (Brandel Inc., Gaithersburg, MD, USA). Radioactivity retained on the filters was counted by liquid scintillation counting using a Beckman Coulter LS6500 (Beckman Coulter Inc., Fullerton, CA, USA). The dissociation constant <i>K</i><sub>d</sub> and maximum binding B<sub>max</sub> values have been previously calculated from the saturation binding studies as 0.87 nM and 7166 fmol mg<sup>−1</sup> protein, respectively. The <i>K</i><sub>d</sub> values for MOR (0.81 nM), DOR (1.7 nM) and NOP receptor (0.17 nM) have been used from previously published literature<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e3444">8</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 52" title="Dumitrascuta, M. et al. Antinociceptive efficacy of the micro-opioid/nociceptin peptide-based Hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking. Molecules 26, 
 https://doi.org/10.3390/molecules26113267
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR52" id="ref-link-section-d112540969e3447">52</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 58" title="Muratspahic, E. et al. Design of a stable cyclic peptide analgesic derived from sunflower seeds that targets the kappa-opioid receptor for the treatment of chronic abdominal pain. J. Med. Chem. 64, 9042–9055 (2021)." href="/articles/s41467-023-43718-w#ref-CR58" id="ref-link-section-d112540969e3450">58</a></sup>.</p><p>The [<sup>35</sup>S]GTPγS binging assay was performed with membranes from CHO-K1 stably expressing the human KOR, DOR or MOR according to the published procedure<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 53" title="Erli, F. et al. Highly potent and selective new diphenethylamines interacting with the kappa-opioid receptor: synthesis, pharmacology, and structure-activity relationships. J. Med. Chem. 60, 7579–7590 (2017)." href="/articles/s41467-023-43718-w#ref-CR53" id="ref-link-section-d112540969e3459">53</a></sup>. Cell membranes (15 µg) in 20 mM HEPES buffer (pH 7.4) supplemented with 10 mM MgCl<sub>2</sub> and 100 mM NaCl were incubated with 0.05 nM [<sup>35</sup>S]GTPγS, 10 µM GDP and various concentrations of test compounds in a final volume of 1 mL for 60 min at 25 °C. Antagonism of DNCP-β-NalA(<b>1</b>) to the MOR was evaluated by Schild regression analysis, with concentration-response curves of DAMGO measured in the absence and presence of DNCP-β-NalA(<b>1</b>) (10, 30 and 100 µM). Non-specific binding was determined using 10 µM GTPγS, and the basal binding was determined in the absence of the test ligand. Samples were filtered over Whatman GF/B glass fiber filters. Filters were washed three times with 5 mL of ice-cold 50 mM Tris-HCl buffer (pH 7.4) using a Brandel M24R cell harvester (Brandel Inc., Gaithersburg, MD, USA). Radioactivity retained on the filters was counted by liquid scintillation counting using a Beckman Coulter LS6500 (Beckman Coulter Inc., Fullerton, CA, USA).</p><p>Functional cAMP assay was performed in a final volume of 20 µL using HEK293T cells stably expressing the mouse KOR<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 58" title="Muratspahic, E. et al. Design of a stable cyclic peptide analgesic derived from sunflower seeds that targets the kappa-opioid receptor for the treatment of chronic abdominal pain. J. Med. Chem. 64, 9042–9055 (2021)." href="/articles/s41467-023-43718-w#ref-CR58" id="ref-link-section-d112540969e3477">58</a></sup>. A number of 2000 cells were seeded into white 384-well plate and incubated with different concentrations of peptide solutions prepared (2X) in 1X stimulation buffer and forskolin (10 µM). The reaction mixture was incubated at 37 °C for 30 min and the reaction was terminated by adding cryptate-labeled cAMP and cAMP d2-labeled antibody. Following an incubation for 1 h at room temperature, cellular cAMP levels were quantified by homogenous time-resolved fluorescence resonance energy transfer (HTRF, ratio 665/620 nm) on a Flexstation 3 (Molecular Devices, San Jose, USA). Bioluminescence resonance energy transfer (BRET)-based β-arrestin-1/2 recruitment was conducted in a final volume of 200 µL using HEK293 cells transiently co-expressing the human β-arrestin-1/2-nano luciferase and the mouse KOR-EGFP (1:10 ratio). After 16–24 h post-transfection, 50,000 cells per well in phenol red-free DMEM supplemented with 10% FBS were transferred into white clear bottom plates and incubated overnight at 37 °C. The following day, cells were subject to serum starvation for 1 h at 37 °C in phenol red-free DMEM. Furimazine, diluted 1:50 in Hank’s balanced salt solution (HBSS) was used as a substrate for nano luciferase. Peptide solutions with indicating concentrations were prepared 4X in HBSS. Prior to establishing the baseline by measuring the signal following addition of furimazine for 5 min at 37 °C, cells were incubated with furimazine for 5 min at 37 °C to allow an efficient cell entry. Subsequently, peptides were incubated at 37 °C for 5 min and the signal was measured on a Flexstation 3 (Molecular Devices, San Jose, USA) for both luminescence at 460 nm (nano luciferase) and fluorescence at 510 nm (EGFP).</p><p>To measure KOR-mediated G protein association of Gα<sub>i1</sub>-, Gα<sub>i2</sub>-, Gα<sub>i3</sub>-, Gα<sub>oA</sub>-, Gα<sub>oB</sub>-, Gα<sub>z</sub>- and Gα<sub>gustducin</sub> containing heterotrimeric G proteins, HEK293T (ATCC, CRL-3216) cells were co-transfected in a 1:1:1:1 ratio with the mouse KOR and the optimal Gα-RLuc8, Gβ, and Gγ-GFP2 subunits according to ref. <sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 35" title="Olsen, R. H. J. et al. TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. Nat. Chem. Biol. 16, 841–849 (2020)." href="/articles/s41467-023-43718-w#ref-CR35" id="ref-link-section-d112540969e3499">35</a></sup>. Briefly, TransIT-2020 (Mirus Bio LLC, Madison, WI, USA) was used to complex the DNA at a ratio of 3 μL Transit per μg DNA, in Opti-MEM at a concentration of 10 ng DNA per μL Opti-MEM. After 16 h, transfected cells were plated in poly-lysine coated 96-well white clear bottom cell culture plates in plating media (DMEM + 1% dialyzed FBS) at a density of 40–50,000 cells in 100 μL per well and incubated overnight. The next day, media was vacuum aspirated, and cells washed twice with 60 μL of assay buffer (20 mM HEPES, 1X HBSS, pH 7.4). Next, 60 μL of the RLuc substrate, coelenterazine 400a (Nanolight Technologies, 5 μM final concentration in assay buffer) was added per well and incubated for 5 min to allow for substrate diffusion. Afterward, 30 μL of test compound (3X) in buffer (20 mM HEPES, 1X HBSS, 0.3% BSA, pH 7.4) were added per well and incubated for another 5 min. Plates were immediately read for both luminescence at 395 nm and fluorescent GFP2 emission at 510 nm for 1 s per well using the FlexStation plate reader. BRET ratios were calculated as the ratio of the GFP2 emission to RLuc8 emission, where the vehicle-treated cell sample represents the background, eliminating the requirement for measuring a donor-only control sample.</p><h3 class="c-article__sub-heading" id="Sec19">Serum stability assay</h3><p>Blood was drawn from healthy volunteers devoid of any medication for 14 days from the antecubital vein using 21 G needles and collected in a serum clot activator tube (4 mL tubes, Greiner bio-one). The study was approved by the Ethical Board of the Medical University of Vienna (EK1548/2020) and conformed to institutional guidelines as well as the Declaration of Helsinki. Volunteers gave written informed consent before blood donation. No sex or gender analysis was conducted as human active serum is expected to have equal activity between the sexes. Blood samples were collected into Vacuette® clot activator tubes (Greiner Bio-One, Austria). After clotting of about 2 h, the samples were centrifuged at 2200 × <i>g</i> for 15 min at 22 °C. The serum was removed and centrifuged again to remove lipids and cell particles at 16,000 × <i>g</i> at 4 °C for 20 min. Peptides, DNCP-β-NalA(<b>1</b>) and dyn A<sub>1-13</sub> (Bachem, Switzerland), were prepared as 500 µM stocks in PBS buffer and diluted 1:10 into the serum sample to give a final concentration of 50 µM. The samples were incubated at 37 °C under continuous shaking at 300 rotations-per-minute. At time points zero, 5, 10, 20, 40, 60, 120, 240, 360, 1440 and 2880 min aliquots of 30 µL were transferred into a low protein binding reaction tube containing protein solubilization buffer (6 M urea in water). The samples were mixed well and incubated on ice for 10 min. For the protein precipitation, 30 µL of a 20% (w/v) trichloroacetic acid was added and incubated on ice for 15 min. The clear supernatant was harvested by centrifugation at 16,000 × <i>g</i> at 4 °C for 20 min and this sample was used for HPLC analysis. The chromatography HPLC system was a ThermoFisher Ultimate 3000 equipped with a pump, autosampler and single wavelength detector. A Phenomenex Kinetex C<sub>18</sub> 150 × 3 mm, 2.6 µm 110 Å was used as separation column with mobile phases 0.1% trifluoroacetic acid in water and acetonitrile/water trifluoroacetic acid 90/10/0.1% (v/v/v) with a linear separation gradient over 30 min. For the evaluation of the remaining peptide peak over time of incubation in active human serum, the area under the curve (AUC) was used with a detection at 214 nm. The remaining peptide was calculated relative to the AUC at time point zero. The data points were fitted to a one-phase decay function and to determine the resulting half-life of the peptide in serum.</p><h3 class="c-article__sub-heading" id="Sec20">Generation of constructs for cryo-EM</h3><p>The human KOR used a construct same as previously determined active-state KOR<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e3537">8</a></sup>. Briefly, the construct (a) lacks N-terminal residues 1–53, (b) lacks C-terminal residues 359–380, (c) contains M1-L106 of the thermostabilized apocytochrome b562 RIL (BRIL) from <i>E. coli</i> in place of receptor N-terminus residues M1-H53. This N-terminal Bril will be removed using a PreScission cleavage site in the end. The single chain Fab scFv16 has the same sequence as previously reported<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 59" title="Maeda, S. et al. Development of an antibody fragment that stabilizes GPCR/G-protein complexes. Nat. Commun. 9, 3712 (2018)." href="/articles/s41467-023-43718-w#ref-CR59" id="ref-link-section-d112540969e3544">59</a></sup>. A 6xHis tag was added to the C-terminal scFv16 sequence with a PreScission cleavage site inserted between. For the Gα<sub>i1</sub> protein heterotrimer, Gα<sub>i1</sub> protein construct was engineered<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 60" title="Liang, Y. L. et al. Ominant negative G proteins enhance formation and purification of agonist-GPCR-G protein complexes for structure determination. ACS Pharmacol. Transl. Sci. 1, 12–20 (2018)." href="/articles/s41467-023-43718-w#ref-CR60" id="ref-link-section-d112540969e3553">60</a></sup> for the binding of scFv16, and then subcloned into a designed vector that co-expresses the Gβ<sub>1</sub> and Gγ<sub>2</sub><sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 61" title="Kim, K. et al. Structure of a hallucinogen-activated Gq-coupled 5-HT2A serotonin receptor. Cell 182, 1574–1588 (2020)." href="/articles/s41467-023-43718-w#ref-CR61" id="ref-link-section-d112540969e3560">61</a></sup>. Further modifications were made to enable a stable complex between KOR, Gα<sub>i1</sub> protein heterotrimer, and scFv16. Specifically, Gα<sub>i1</sub>-dominant negative variants are S47N, E245A, G203A, and A326S.</p><h3 class="c-article__sub-heading" id="Sec21">Expression of KOR-Gα<sub>i1</sub> protein-scFv16 complex</h3><p>The Bac-to-Bac Baculovirus Expression System was applied to generate high-quality recombinant baculovirus for protein expression<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e3579">8</a></sup>. For the expression of KOR-Gα<sub>i1</sub>-scFv16 protein complex, Gα<sub>i1</sub>, Gβ<sub>1</sub>, and Gγ<sub>2</sub> were coexpressed with KOR and scFv16, respectively, by infection <i>Spodoptera frugiperda</i> Sf9 cells (Expression Systems, 94-001 S) at a cell density of 2.5 × 10<sup>6</sup> cells per mL in ESF921 medium (Expression Systems, Davies, CA, USA) with the P1 baculovirus at a multiplicity of infection (MOI) ratio of 2:2:0.5. Cells were harvested by centrifugation after infection for 48 h (125 rotations-per-minute at 27 °C), washed with HN buffer (25 mM HEPES pH 7.4, 100 mM NaCl), and stored at −80 °C for further purification.</p><h3 class="c-article__sub-heading" id="Sec22">Purification of the KOR-G<sub>i1</sub> protein-scFv16 complex</h3><p>The thawed cell pellet was incubated in buffer containing 20 mM HEPES 7.5, 50 mM NaCl, 1 mM MgCl<sub>2</sub>, 2.5 U apyrase (NEB), 10 μM agonist (final concentration) and protease inhibitors (500 mM AEBSF, 1 mM E-64, 1 mM leupeptin, 150 nM aprotinin) for 1.5 h at room temperature. Then, harvested the membrane by centrifugation at 64,300 × g for 30 min at 4 °C. The membrane was solubilized in the buffer (40 mM HEPES pH7.5, 100 mM NaCl, 5% (w/v) glycerol, 0.6% (w/v) lauryl maltose neopentyl glycol (LMNG), 0.06% (w/v) cholesteryl hemisuccinate (CHS), 10 μM agonist and protease inhibitors) with 200 μg scFv16 in the cold room. After 5 h, the supernatant was collected by centrifugation at 92,600 × <i>g</i> for 30 min at 4 °C and incubated with 1 mL TALON IMAC resin (Clontech, now Takara Bio Inc, San Jose, CA, USA) and 20 mM imidazole overnight in the cold room. The next day, the resin was collected and washed with 10 ml buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 30 mM imidazole, 0.01% (w/v) LMNG, 0.001% (w/v) CHS, 5% glycerol and 5 μM agonist. Then the protein was eluted with the same buffer supplemented with 300 mM imidazole, concentrated and further purified by size-exclusion chromatography (SEC) on the Superdex 200 increase 10/300 column (GE healthcare, WI, USA) that is pre-equilibrated with 20 mM HEPES pH7.5, 100 mM NaCl, 100 μM TCEP, 0.00075% (w/v) LMNG, 0.00025 (w/v) glyco-diosgenin (GDN) and 0.00075% (w/v) CHS, 1 μM agonist. Peak fractions were collected, concentrated and incubated with PNGase F (NEB, MA, USA), PreScission protease (GeneScript, NJ, USA) to remove the potential glycosylation and N-terminal his-BRIL, respectively, and 100 μg scFv16 at 4 °C overnight. In the next day, cleaved his-Bril and protein, uncleaved protein and proteases were separated by the same procedure as described above. Peak fractions were concentrated to 3–5 mg mL<sup>−1</sup> for electron microscope studies.</p><h3 class="c-article__sub-heading" id="Sec23">Expression and purification of scFv16 protein</h3><p>The scFv16 protein was expressed by infection Sf9 cells (Expression Systems 94-001 S). at a cell density of 2.5 × 10<sup>6</sup> cells per mL in ESF921 medium (Expression Systems) with the P1 baculovirus at a MOI of 2. After 96 h, the cell cultured medium containing secreted scFv16 protein was collected by centrifugation at 3739 × <i>g</i> for 15 min. The pH of supernatant was adjusted to 7.5 by addition of Tris-base power. Chelating agents were quenched by addition of 1 mM nickel chloride and 5 mM calcium chloride and incubation with stirring for 1 h at room temperature and 5 h in the cold room further. Removed the precipitates by centrifugation and the resultant supernatant was further cleaned with 0.45 μm filter paper and incubated with 2 mL Ni-NTA resin and 10 mM imidazole overnight in the cold room. The Ni-NTA resin was wash next day with 20 mL buffer of 20 mM HEPES pH7.5, 100 mM NaCl, 0.00075% (w/v) LMNG, 0.000075% (w/v) CHS, 0.00025% (w/v) GDN, 20 mM imidazole. The protein was eluted with the same buffer supplemented with 300 mM imidazole, concentrated and further purified on a Superdex 200 increase 10/300 column. Monomeric fractions were pooled, concentrated, flash frozen in liquid nitrogen and stored at −80 °C until future use.</p><h3 class="c-article__sub-heading" id="Sec24">Cryo-EM data collection and 3D reconstruction</h3><p>The purified samples (3–4 μL) were applied to glow-discharged 300-mesh Au grids (Quantifoil R1.2/1.3) individually and vitrified using a Vitt mark IV (ThermoFisher). Cryo-EM imaging was performed on a Talos Artica operated at 200 kV at a nominal magnification of 45,000-times using a Gatan K3 direct electron detector at a physical pixel size of 0.88 Å. Each stack movie was recorded for 2 to 2.7 s in 60 frames at a dose rate of ~15 e<sup>−</sup>/pix/sec. Movies were collected automatically with SerialEM63 using an optimized multishot array procedure<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 62" title="Peck, J. V., Fay, J. F. & Strauss, J. D. High-speed high-resolution data collection on a 200 keV cryo-TEM. IUCrJ 9, 243–252 (2022)." href="/articles/s41467-023-43718-w#ref-CR62" id="ref-link-section-d112540969e3638">62</a></sup>. Dose-fractioned image stacks were subjected to the beam-induced motion correction followed by contrast transfer function (CTF) estimation. Particles were selected by Blob particle picker, extracted from micrograph and then used for 2D classification, 3D classification followed by non-uniform refinement. All these steps were performed in cryoSPARC<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 63" title="Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290–296 (2017)." href="/articles/s41467-023-43718-w#ref-CR63" id="ref-link-section-d112540969e3642">63</a>,<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 64" title="Punjani, A., Zhang, H. & Fleet, D. J. Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction. Nat. Methods 17, 1214–1221 (2020)." href="/articles/s41467-023-43718-w#ref-CR64" id="ref-link-section-d112540969e3645">64</a></sup>.</p><h3 class="c-article__sub-heading" id="Sec25">Model building and refinement</h3><p>Maps from cryoSPARC were used for map building, refinement and subsequent structural interpretation. The dominant-negative Gα<sub>i1</sub> trimer model and scFv16 model were adapted from the cryo-EM structure of the MRGPRX2–G Gα<sub>i1</sub> complex (<a href="https://doi.org/10.2210/pdb7S8M/pdb">PDB: 7S8M</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 65" title="Cao, C. et al. Structure, function and pharmacology of human itch GPCRs. Nature 600, 170–175 (2021)." href="/articles/s41467-023-43718-w#ref-CR65" id="ref-link-section-d112540969e3668">65</a></sup>. The receptor KOR model was taken from the active-state KOR-Nb39 structure (<a href="https://doi.org/10.2210/pdb6B73/pdb">PDB: 6B73</a>)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. Cell 172, 55–67 (2018)." href="/articles/s41467-023-43718-w#ref-CR8" id="ref-link-section-d112540969e3680">8</a></sup>. The receptor, Gα<sub>i1</sub> protein, and scFv16 were docked into the cryo-EM map using Chimera<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 66" title="Pettersen, E. F. et al. UCSF Chimera–a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605–1612 (2004)." href="/articles/s41467-023-43718-w#ref-CR66" id="ref-link-section-d112540969e3686">66</a></sup>. The complex model (KOR-Gα<sub>i1</sub> protein-scFv16) were manually built in Coot<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 67" title="Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 66, 486–501 (2010)." href="/articles/s41467-023-43718-w#ref-CR67" id="ref-link-section-d112540969e3692">67</a></sup>, followed by several rounds of real-space refinement using Phenix<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 68" title="Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213–221 (2010)." href="/articles/s41467-023-43718-w#ref-CR68" id="ref-link-section-d112540969e3696">68</a></sup>. The model statistics were validated using Molprobity<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 69" title="Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr. D Biol. Crystallogr. 66, 12–21 (2010)." href="/articles/s41467-023-43718-w#ref-CR69" id="ref-link-section-d112540969e3701">69</a></sup>. Structural figures were prepared using Chimera or Pymol (<a href="https://pymol.org/2/">https://pymol.org/2/</a>).</p><h3 class="c-article__sub-heading" id="Sec26">Molecular dynamics simulations</h3><p>We performed an all-atomistic molecular dynamics simulation of KOR-DNCP-β-NalA(<b>1</b>) complex using the Compute Unified Device Architecture (CUDA) version of particle-mesh Ewald molecular dynamics (PMEMD) on graphics processing units (GPUs) in AMBER22<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 70" title="Case, D. A. et al. Amber 2023 (University of California, 2023)." href="/articles/s41467-023-43718-w#ref-CR70" id="ref-link-section-d112540969e3724">70</a></sup>. The initial coordinate of G proteins (Gα<sub>i1</sub>/Gβ<sub>1</sub>/Gγ<sub>2</sub>) and KOR bound with DNCP-β-NalA(<b>1</b>) is reported in this manuscript. The ICL and ECL missing loops of KOR are modeled by aligning KOR on <a href="https://doi.org/10.2210/pdb8F7W/pdb">PDB: 8F7W</a><sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 38" title="Wang, Y. et al. Structures of the entire human opioid receptor family. Cell 186, 413–427, (2023)." href="/articles/s41467-023-43718-w#ref-CR38" id="ref-link-section-d112540969e3744">38</a></sup> as template structure. The missing domain of guanine nucleotide-binding protein Gα<sub>i1</sub> is modeled using Alpha Fold (UniProt: P63096)<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 71" title="Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583–589 (2021)." href="/articles/s41467-023-43718-w#ref-CR71" id="ref-link-section-d112540969e3750">71</a></sup>. All missing atoms in Gβ<sub>1</sub>, Gγ<sub>2</sub> proteins and KOR are added using TLEAP implemented in AMBER22 and the missing hydrogens in DNCP-β-NalA(<b>1</b>) are added by Open-Babel<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 46" title="O’Boyle, N. M. et al. Open Babel: an open chemical toolbox. J. Cheminform. 3, 33 (2011)." href="/articles/s41467-023-43718-w#ref-CR46" id="ref-link-section-d112540969e3762">46</a></sup>. Then the model G proteins-KOR-DNCP-β-NalA(<b>1</b>) complex was embedded in a lipid bilayer membrane using PACKMOL-Memgen module<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 72" title="Schott-Verdugo, S. & Gohlke, H. PACKMOL-Memgen: a simple-to-use, generalized workflow for membrane-protein-lipid-bilayer system building. J. Chem. Inf. Model 59, 2522–2528 (2019)." href="/articles/s41467-023-43718-w#ref-CR72" id="ref-link-section-d112540969e3769">72</a></sup> of AmberTool23<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 70" title="Case, D. A. et al. Amber 2023 (University of California, 2023)." href="/articles/s41467-023-43718-w#ref-CR70" id="ref-link-section-d112540969e3773">70</a></sup>. The lipid composition 0.55:0.15:0.30 ratio is used for the construction of lipid bilayer membrane in the ratio of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) and cholesterol (CHL1), respectively. Similar lipid composition ratio was reported previously<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 73" title="Han, J. et al. Ligand and G-protein selectivity in the kappa-opioid receptor. Nature 617, 417–425 (2023)." href="/articles/s41467-023-43718-w#ref-CR73" id="ref-link-section-d112540969e3778">73</a></sup>. The final KOR-DNCP-β-NalA(<b>1</b>) complex with G proteins contained 330 DPPC, 90 DOPC and 180 CHL1 lipids, 63,537 water molecules, and 172 sodium and 172 chloride ions (Supplementary Table <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM1">15</a>). The Amberff19SB<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 74" title="Tian, C. et al. ff19SB: amino-acid-specific protein backbone parameters trained against quantum mechanics energy surfaces in solution. J. Chem. Theory Comput. 16, 528–552 (2020)." href="/articles/s41467-023-43718-w#ref-CR74" id="ref-link-section-d112540969e3788">74</a></sup> and Lipid21<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 75" title="Dickson, C. J., Walker, R. C. & Gould, I. R. Lipid21: complex lipid membrane simulations with AMBER. J. Chem. Theory Comput. 18, 1726–1736 (2022)." href="/articles/s41467-023-43718-w#ref-CR75" id="ref-link-section-d112540969e3792">75</a></sup> force field parameters are used for protein and lipid molecules, respectively. The DNCP-β-NalA(<b>1</b>) interacting potential parameters are generated using GAFF2 force field<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 76" title="Wang, J., Wolf, R. M., Caldwell, J. W., Kollman, P. A. & Case, D. A. Development and testing of a general amber force field. J. Comput. Chem 25, 1157–1174 (2004)." href="/articles/s41467-023-43718-w#ref-CR76" id="ref-link-section-d112540969e3800">76</a></sup> and the columbic charge of DNCP-β-NalA(<b>1</b>) atoms is assigned through Antechamber at AM1BCC scheme. Water is modeled using TIP3P water model<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 77" title="Jorgensen, W. L., Chandrasekhar, J., Madura, J. D., Impey, R. W. & Klein, M. L. Comparison of simple potential functions for simulating liquid water. J. Chem. Phys. 79, 926–935 (1983)." href="/articles/s41467-023-43718-w#ref-CR77" id="ref-link-section-d112540969e3807">77</a></sup> and counter-ions are model using the Joung/Cheatham parameters<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 78" title="Joung, I. S. & Cheatham, T. E. 3rd Determination of alkali and halide monovalent ion parameters for use in explicitly solvated biomolecular simulations. J. Phys. Chem. B 112, 9020–9041 (2008)." href="/articles/s41467-023-43718-w#ref-CR78" id="ref-link-section-d112540969e3811">78</a></sup>. The system is minimized for 10,000 steps, the first 5000 step using steepest descent method and the next 5000 steps using conjugate gradient method using 5 kcal<sup>−1</sup> mol<sup>−1</sup> Å<sup>−2</sup> on non-solvent molecules. The minimized structure is heated from a 0 K to 310 K in 100 ps using NVT ensemble by putting restraints of 5 kcal mol<sup>−1</sup> Å<sup>−2</sup> on non-solvent molecules, followed by 1 ns NPT equilibration with restraints on non-solvent molecules. The system was further equilibrated for another 30 ns a in NPT ensemble using 5 kcal mol<sup>−1</sup> Å<sup>−2</sup> restraint only on backbone of G proteins and KOR-DNCP-β-NalA(<b>1</b>) complex allowing equilibration of lipid and solvent molecules. The restraints were removed in 5 steps from 5 kcal mol<sup>−1</sup> Å<sup>−2</sup> to 0 kcal mol<sup>−1</sup> Å<sup>−2</sup> in a stepwise of 2 ns each step. The 100 ns equilibration run is performed without restraints at 1 atm pressure and 310 K temperature, using the Langevin thermostat with collision frequency of 2 ps<sup>−1</sup> and the Monte Carlo barostat with semi-isotropic pressure scaling using 2 fs time step. The particle-mesh Ewald (PME) method is used to calculate long-range electrostatic interactions. A cut-off 10 Å is used for all non-bonded interactions. Four independent 500 ns production run is started from last frame of equilibrated system using identical setting except the hydrogen mass repartitioning was used at a 4 fs time step<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 79" title="Best, R. B., Hummer, G. & Eaton, W. A. Native contacts determine protein folding mechanisms in atomistic simulations. Proc. Natl Acad. Sci. USA 110, 17874–17879 (2013)." href="/articles/s41467-023-43718-w#ref-CR79" id="ref-link-section-d112540969e3844">79</a></sup>. The trajectories were saved at an interval of 10 ps. AmberTools 23<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 70" title="Case, D. A. et al. Amber 2023 (University of California, 2023)." href="/articles/s41467-023-43718-w#ref-CR70" id="ref-link-section-d112540969e3849">70</a></sup> and VMD<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 80" title="Humphrey, W., Dalke, A. & Schulten, K. VMD: visual molecular dynamics. J.Mol. Graph. 14, 27–38 (1996)." href="/articles/s41467-023-43718-w#ref-CR80" id="ref-link-section-d112540969e3853">80</a></sup> were used for analysis and visualization, respectively. The fraction of contacts between DNCP-β-NalA(<b>1</b>) and the KOR is used to quantify inter-atomic interactions within the complex. These contacts are defined by applying a distance cutoff of 4.5 Å between all heavy atoms of DNCP-β-NalA(<b>1</b>) and all heavy atoms of amino acids in KOR. Hydrogen bonds between residue pairs are calculated using a criteria of acceptor-to-donor heavy atom distance of 3.5 Å and an angle of 135°. The combined 2 μs simulation trajectories are clustered together using the k-means clustering algorithm based on DNCP-β-NalA(<b>1</b>) heavy atom root-mean-square deviation (RMSD) to extract a representative conformation of the KOR-DNCP-β-NalA(<b>1</b>) complex, implemented in AmberTools23.</p><h3 class="c-article__sub-heading" id="Sec27">Enzyme-linked immunosorbent assay (ELISA)</h3><p>To determine the expression level of constructs KOR and its mutants, HEK293T cells were plated in poly-L-lysine-coated 96-well white clear bottom cell culture plate at a density of 20,000–30,000 cells per well overnight and transfected with 300 ng plasmids for each well. After 24 h, plates were decanted and fixed with 4% (w/v) paraformaldehyde for 10 min at room temperature. Then, cells were washed twice with 1X PBS (phosphate buffered saline, pH 7.4) and blocked by 1X PBS containing 0.5% (w/v) non-fat milk for at least 30 min at room temperature followed by incubation with anti-Flag (M2)–horseradish peroxidase-conjugated antibody (Sigma-Aldrich, A8592) diluted 1:20,000 in the same buffer for 1 h at room temperature. After washing three times with 1X PBS, 1-StepTM Ultra-TMB ELISA substrate (ThermoFisher, 34028) was added to the plates and incubated at 37 °C for 15–30 min and terminated by addition of 1 M sulfuric acid (H<sub>2</sub>SO<sub>4</sub>) stop solution. Finally, plates were read at the wavelength of 450 nm with the BioTek Luminescence reader.</p><h3 class="c-article__sub-heading" id="Sec28">In vivo pharmacology</h3><p>The formalin test<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 81" title="Dubuisson, D. & Dennis, S. G. The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. Pain 4, 161–174 (1977)." href="/articles/s41467-023-43718-w#ref-CR81" id="ref-link-section-d112540969e3889">81</a></sup> was performed in male SWISS mice (8–10 weeks old, 30–35 g body weight, <i>n</i> = 50 mice). Following a habituation period of 15 min to individual transparent observation chambers, male mice were s.c. administered saline (control), DNCP-β-NalA(<b>1</b>) (0.8, 1.9 and 3.8 µmol kg<sup>−1</sup>) or U50,488 (1.1, 2.1 and 5.4 µmol kg<sup>−1</sup>), 15 min prior injection of 20 µL of 5% formalin aqueous solution to the plantar surface of the right hindpaw. The time (in sec) each animal spent licking, biting, lifting, and flinching of the formalin-injected paw (pain behavior) was recorded in 5 min intervals between 15 and 30 min after the injection of formalin (Phase II reaction). For the antagonism study, nor-BNI (13.6 µmol kg<sup>−1</sup>) was s.c. administered 24 h before DNCP-β-NalA(<b>1</b>) (3.8 µmol kg<sup>−1</sup>) and pain behavior was assessed as described above. Antinociceptive activity in the formalin test, as percentage decrease in duration of pain behavior compared to the saline (control) group, was calculated according to the following formula: <span class="mathjax-tex">\(100*[\frac{C-T}{C}]\)</span>, where C is the mean time in control (saline) group and T is the time in drug-treated group<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 82" title="Puls, K. et al. In vitro, In vivo and In silico Characterization of a Novel Kappa-opioid receptor antagonist. Pharmaceuticals 15, 
 https://doi.org/10.3390/ph15060680
 
 (2022)." href="/articles/s41467-023-43718-w#ref-CR82" id="ref-link-section-d112540969e3959">82</a></sup>. Dose-response relationships of percentage inhibition of pain behavior were constructed, and the dose necessary to produce a 50% effect (<i>ED</i><sub>50</sub>) and 95% confidence limits (95% CL) was calculated according to the method of Litchfield and Wilcoxon<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 83" title="Litchfield, J. T. Jr. & Wilcoxon, F. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther. 96, 99–113 (1949)." href="/articles/s41467-023-43718-w#ref-CR83" id="ref-link-section-d112540969e3968">83</a></sup>.</p><p>Sixty min after injection of formalin to male mice, maximal paw thickness of the right hind paw (i.e., formalin-injected hind paw) was measured using a digital micrometer (FixPoint, Vienna, Austria). Data are expressed in mm.</p><p>CFA-induced inflammatory hyperalgesia was induced in male SWISS mice (8–10 weeks old, 30–35 g body weight, <i>n</i> = 53 mice) by s.c. injection of 20 µL emulsified CFA (1 mg mL<sup>−1</sup>) into the plantar surface of the right hindpaw under brief isoflurane anesthesia<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 52" title="Dumitrascuta, M. et al. Antinociceptive efficacy of the micro-opioid/nociceptin peptide-based Hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking. Molecules 26, 
 https://doi.org/10.3390/molecules26113267
 
 (2021)." href="/articles/s41467-023-43718-w#ref-CR52" id="ref-link-section-d112540969e3983">52</a></sup>. Nociceptive testing was performed 72 h after inoculation with CFA. Hindpaws withdrawal latencies to thermal stimulation were measured using the Hargreaves test<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 84" title="Hargreaves, K., Dubner, R., Brown, F., Flores, C. & Joris, J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 32, 77–88 (1988)." href="/articles/s41467-023-43718-w#ref-CR84" id="ref-link-section-d112540969e3987">84</a></sup> with an analgesiometer (Ugo Basile S.R.L., Varese, Italy). Male mice were habituated in individual Plexiglas boxes positioned on a glass surface (Ugo Basile S.R.L., Varese, Italy) for three days prior to the inoculation with CFA. A movable infrared generator (30% intensity) located under the glass floor was focused onto the plantar surface of the hindpaw and switched on to heat. Onset of the radiant stimulus triggered a timer, which was stopped by subsequent paw movement. To prevent tissue damage, a cut-off time of 20 s was imposed. Thermal sensitivity was measured before inoculation with CFA into the right hindpaw (basal latencies, BL), 72 h post-inoculation (pre-treatment values, defined as 0 h), and at different time points after s.c. administration of saline (control), DNCP-β-NalA(<b>1</b>) (0.4, 0.8 and 1.9 µmol kg<sup>−1</sup>) or U50,488 (0.2, 0.6 and 2.1 µmol kg<sup>−1</sup>). For the antagonism study, nor-BNI (13.6 µmol kg<sup>−1</sup>) was s.c. administered 24 h before DNCP-β-NalA(<b>1</b>) (1.9 µmol kg<sup>−1</sup>) and pain behavior was assessed. The paw withdrawal latencies to thermal stimulus was expressed in seconds.</p><p>The rotarod test was used to evaluate coordinated locomotion and potential sedation induced by test drugs<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 85" title="Jones, B. J. & Roberts, D. J. The quantiative measurement of motor inco-ordination in naive mice using an acelerating rotarod. J. Pharm. Pharmacol 20, 302–304 (1968)." href="/articles/s41467-023-43718-w#ref-CR85" id="ref-link-section-d112540969e4009">85</a></sup>. The accelerating rotarod treadmill (Acceler Rota-Rod 7650, Ugo Basile s.r.l., Varese, Italy) for mice (diameter 3.5 cm) was used. Male SWISS mice (8–10 weeks old, 30–35 g body weight, <i>n</i> = 21 mice) were habituated to the equipment in two training sessions (30 min apart) one day before testing. On the experimental day, male mice were placed on the rotarod, and treadmill was accelerated from 4 to 40 rotations-per-minute over a period of 5 min. The time spent on the drum was recorded for each mouse before (baseline) and at 30, 60 and 120 min after s.c. administration of saline (control), DNCP-β-NalA(<b>1</b>) (3.8 and 7.6 µmol kg<sup>−1</sup>) or U50,488 (5.4 µmol kg<sup>−1</sup>). Decreased latencies to fall in the rotarod test indicate impaired motor performance. A 300 s cut-off time was used. The rotarod data are expressed as percentage (%) changes from the rotarod latencies obtained before (baseline) and after drug administration (test, T) were calculated as <span class="mathjax-tex">\(100*(\frac{T}{B})\)</span><sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 53" title="Erli, F. et al. Highly potent and selective new diphenethylamines interacting with the kappa-opioid receptor: synthesis, pharmacology, and structure-activity relationships. J. Med. Chem. 60, 7579–7590 (2017)." href="/articles/s41467-023-43718-w#ref-CR53" id="ref-link-section-d112540969e4067">53</a></sup>.</p><h3 class="c-article__sub-heading" id="Sec29">Data analysis</h3><p>In vitro and in vivo data analyses were carried out with GraphPad Prism (GraphPad Software, San Diego). Data are presented as means ± s.e.m (unless otherwise stated). Data from cAMP or β-arrestin recruitment or G protein biosensor assays were fitted to three-parameter non-linear regression curves with a bottom constrained to zero, a slope of one and logarithmic scale. The observed sigmoidal curves were normalized to 100% which refers to the saturating concentration of full KOR agonists U50,488, U69,593 or dyn A<sub>1-13</sub> (for KOR), DAMGO (for MOR) and DPDPE or DADLE (for DOR) (10 µM each) (unless otherwise stated). Data from radioligand binding studies were fitted to a three-parameter logistic Hill equation to derive the <i>IC</i><sub>50</sub> and inhibition constants (<i>K</i><sub>i</sub>) calculated by the approximation of Cheng and Prusoff<sup><a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 86" title="Cheng, Y. & Prusoff, W. H. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22, 3099–3108 (1973)." href="/articles/s41467-023-43718-w#ref-CR86" id="ref-link-section-d112540969e4089">86</a></sup>. Specific binding data were normalized to 100% of [<sup>3</sup>H]DPN (KOR, MOR, and DOR) or [<sup>3</sup>H]nociceptin (NOP receptor) (no competing ligand). The 100% defines an average of 4000–6000 fmol mg<sup>−1</sup> of protein per assay for KOR, 500–1000 fmol mg<sup>−1</sup> of protein for MOR, 1500 fmol mg<sup>−1</sup> of protein for DOR and 150–300 fmol mg<sup>−1</sup> for NOP receptor. The increase in [<sup>35</sup>S]GTPγS binding above the basal activity was used to determine potency (<i>EC</i><sub>50</sub>, in nM) and efficacy (as <i>E</i><sub>max</sub> in %, as maximum stimulation relative to the reference full KOR agonist U69,593) from concentration-response curves by nonlinear regression analysis. For Schild regression analysis, data were normalized to 100%, i.e., the highest concentration (10 μM) of DAMGO and fitted to three-parameter non-linear regression curves. The logarithm of the concentration-ratio <span class="mathjax-tex">\(\frac{A{\prime} }{A-1}\)</span> (A denotes <i>EC</i><sub><i>50</i></sub> of DAMGO in presence of DNCP-β-NalA(<b>1</b>); A′ is <i>EC</i><sub><i>50</i></sub> of DAMGO) was plotted against the logarithm of the respective concentration of antagonist DNCP-β-NalA(<b>1</b>) to derive the pA2 value (functional inhibitory affinity). All in vitro assays were performed in duplicates or triplicates and repeated at least three times with independently prepared samples. In vivo data were statistically evaluated using one-way ANOVA with Dunnett’s or Tukey’s multiple comparison post hoc test, or two-way ANOVA with Bonferroni’s post hoc test with significance set at <i>P</i> < 0.05.</p><h3 class="c-article__sub-heading" id="Sec30">Reporting summary</h3><p>Further information on research design is available in the <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-023-43718-w#MOESM8">Nature Portfolio Reporting Summary</a> linked to this article.</p></div></div></section> </div> <div> <section data-title="Data availability"><div class="c-article-section" id="data-availability-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="data-availability">Data availability</h2><div class="c-article-section__content" id="data-availability-content"> <p>The data supporting this study are available from the corresponding authors upon request. The cryo-EM maps and corresponding coordinates have been deposited in the Electron Microscopy Data Bank (EMDB) under accession codes <a href="https://www.ebi.ac.uk/pdbe/entry/emdb/EMD-29026">EMD-29026</a> and the Protein Data Bank (PDB) under accession codes <a href="http://doi.org/10.2210/pdb8FEG/pdb">8FEG</a>. The script data generated in this study are provided as Supplementary Data files. Source data are provided with this paper in the Source Data file and from the corresponding authors upon request. <a data-track="click" data-track-label="link" data-track-action="section anchor" href="/articles/s41467-023-43718-w#Sec32">Source data</a> are provided with this paper.</p> </div></div></section><section data-title="Code availability"><div class="c-article-section" id="code-availability-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="code-availability">Code availability</h2><div class="c-article-section__content" id="code-availability-content"> <p>The codes used for design and instructions on how to run, as well as the Molecular Dynamics (MD) simulation initial coordinate, simulation input files and a coordinate file of the final output can be found in kappa_SMpeptide_design folder in a github repository (<a href="https://github.com/kdeibler/kappa_SMpeptide_deisgn">https://github.com/kdeibler/kappa_SMpeptide_deisgn</a>; generated <a href="https://doi.org/10.5281/zenodo.10070208">https://doi.org/10.5281/zenodo.10070208</a>).</p> </div></div></section><div id="MagazineFulltextArticleBodySuffix"><section aria-labelledby="Bib1" data-title="References"><div class="c-article-section" id="Bib1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Bib1">References</h2><div class="c-article-section__content" id="Bib1-content"><div data-container-section="references"><ol class="c-article-references" data-track-component="outbound reference" data-track-context="references section"><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="1."><p class="c-article-references__text" id="ref-CR1">Davenport, A. P., Scully, C. C. G., de Graaf, C., Brown, A. J. H. & Maguire, J. J. Advances in therapeutic peptides targeting G protein-coupled receptors. <i>Nat. Rev. Drug Discov.</i> <b>19</b>, 389–413 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41573-020-0062-z" data-track-item_id="10.1038/s41573-020-0062-z" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41573-020-0062-z" aria-label="Article reference 1" data-doi="10.1038/s41573-020-0062-z">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXlt1Ciu70%3D" aria-label="CAS reference 1">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32494050" aria-label="PubMed reference 1">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 1" href="http://scholar.google.com/scholar_lookup?&title=Advances%20in%20therapeutic%20peptides%20targeting%20G%20protein-coupled%20receptors&journal=Nat.%20Rev.%20Drug%20Discov.&doi=10.1038%2Fs41573-020-0062-z&volume=19&pages=389-413&publication_year=2020&author=Davenport%2CAP&author=Scully%2CCCG&author=Graaf%2CC&author=Brown%2CAJH&author=Maguire%2CJJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="2."><p class="c-article-references__text" id="ref-CR2">Hauser, A. S., Attwood, M. M., Rask-Andersen, M., Schioth, H. B. & Gloriam, D. E. Trends in GPCR drug discovery: new agents, targets and indications. <i>Nat. Rev. Drug Discov.</i> <b>16</b>, 829–842 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nrd.2017.178" data-track-item_id="10.1038/nrd.2017.178" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fnrd.2017.178" aria-label="Article reference 2" data-doi="10.1038/nrd.2017.178">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXhslansbzF" aria-label="CAS reference 2">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=29075003" aria-label="PubMed reference 2">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882681" aria-label="PubMed Central reference 2">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 2" href="http://scholar.google.com/scholar_lookup?&title=Trends%20in%20GPCR%20drug%20discovery%3A%20new%20agents%2C%20targets%20and%20indications&journal=Nat.%20Rev.%20Drug%20Discov.&doi=10.1038%2Fnrd.2017.178&volume=16&pages=829-842&publication_year=2017&author=Hauser%2CAS&author=Attwood%2CMM&author=Rask-Andersen%2CM&author=Schioth%2CHB&author=Gloriam%2CDE"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="3."><p class="c-article-references__text" id="ref-CR3">Muratspahic, E., Freissmuth, M. & Gruber, C. W. Nature-derived peptides: a growing niche for GPCR ligand discovery. <i>Trends Pharmacol. Sci.</i> <b>40</b>, 309–326 (2019).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.tips.2019.03.004" data-track-item_id="10.1016/j.tips.2019.03.004" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2Fj.tips.2019.03.004" aria-label="Article reference 3" data-doi="10.1016/j.tips.2019.03.004">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1MXlvVWnsr8%3D" aria-label="CAS reference 3">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=30955896" aria-label="PubMed reference 3">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6624132" aria-label="PubMed Central reference 3">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 3" href="http://scholar.google.com/scholar_lookup?&title=Nature-derived%20peptides%3A%20a%20growing%20niche%20for%20GPCR%20ligand%20discovery&journal=Trends%20Pharmacol.%20Sci.&doi=10.1016%2Fj.tips.2019.03.004&volume=40&pages=309-326&publication_year=2019&author=Muratspahic%2CE&author=Freissmuth%2CM&author=Gruber%2CCW"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="4."><p class="c-article-references__text" id="ref-CR4">Muttenthaler, M., King, G. F., Adams, D. J. & Alewood, P. F. Trends in peptide drug discovery. <i>Nat. Rev. Drug Discov.</i> <b>20</b>, 309–325 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41573-020-00135-8" data-track-item_id="10.1038/s41573-020-00135-8" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41573-020-00135-8" aria-label="Article reference 4" data-doi="10.1038/s41573-020-00135-8">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXjtF2qsro%3D" aria-label="CAS reference 4">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=33536635" aria-label="PubMed reference 4">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 4" href="http://scholar.google.com/scholar_lookup?&title=Trends%20in%20peptide%20drug%20discovery&journal=Nat.%20Rev.%20Drug%20Discov.&doi=10.1038%2Fs41573-020-00135-8&volume=20&pages=309-325&publication_year=2021&author=Muttenthaler%2CM&author=King%2CGF&author=Adams%2CDJ&author=Alewood%2CPF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="5."><p class="c-article-references__text" id="ref-CR5">Volkow, N. D. & Collins, F. S. The role of science in addressing the opioid crisis. <i>N. Engl. J. Med.</i> <b>377</b>, 391–394 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1056/NEJMsr1706626" data-track-item_id="10.1056/NEJMsr1706626" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1056%2FNEJMsr1706626" aria-label="Article reference 5" data-doi="10.1056/NEJMsr1706626">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=28564549" aria-label="PubMed reference 5">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 5" href="http://scholar.google.com/scholar_lookup?&title=The%20role%20of%20science%20in%20addressing%20the%20opioid%20crisis&journal=N.%20Engl.%20J.%20Med.&doi=10.1056%2FNEJMsr1706626&volume=377&pages=391-394&publication_year=2017&author=Volkow%2CND&author=Collins%2CFS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="6."><p class="c-article-references__text" id="ref-CR6">Darcq, E. & Kieffer, B. L. Opioid receptors: drivers to addiction? <i>Nat. Rev. Neurosci.</i> <b>19</b>, 499–514 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41583-018-0028-x" data-track-item_id="10.1038/s41583-018-0028-x" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41583-018-0028-x" aria-label="Article reference 6" data-doi="10.1038/s41583-018-0028-x">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1cXht1Smt7rJ" aria-label="CAS reference 6">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=29934561" aria-label="PubMed reference 6">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 6" href="http://scholar.google.com/scholar_lookup?&title=Opioid%20receptors%3A%20drivers%20to%20addiction%3F&journal=Nat.%20Rev.%20Neurosci.&doi=10.1038%2Fs41583-018-0028-x&volume=19&pages=499-514&publication_year=2018&author=Darcq%2CE&author=Kieffer%2CBL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="7."><p class="c-article-references__text" id="ref-CR7">Del Vecchio, G., Spahn, V. & Stein, C. Novel opioid analgesics and side effects. <i>ACS Chem. Neurosci.</i> <b>8</b>, 1638–1640 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acschemneuro.7b00195" data-track-item_id="10.1021/acschemneuro.7b00195" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facschemneuro.7b00195" aria-label="Article reference 7" data-doi="10.1021/acschemneuro.7b00195">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=28603962" aria-label="PubMed reference 7">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 7" href="http://scholar.google.com/scholar_lookup?&title=Novel%20opioid%20analgesics%20and%20side%20effects&journal=ACS%20Chem.%20Neurosci.&doi=10.1021%2Facschemneuro.7b00195&volume=8&pages=1638-1640&publication_year=2017&author=Vecchio%2CG&author=Spahn%2CV&author=Stein%2CC"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="8."><p class="c-article-references__text" id="ref-CR8">Che, T. et al. Structure of the nanobody-stabilized active state of the kappa opioid receptor. <i>Cell</i> <b>172</b>, 55–67 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.cell.2017.12.011" data-track-item_id="10.1016/j.cell.2017.12.011" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2Fj.cell.2017.12.011" aria-label="Article reference 8" data-doi="10.1016/j.cell.2017.12.011">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1cXkslehtg%3D%3D" aria-label="CAS reference 8">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=29307491" aria-label="PubMed reference 8">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802374" aria-label="PubMed Central reference 8">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 8" href="http://scholar.google.com/scholar_lookup?&title=Structure%20of%20the%20nanobody-stabilized%20active%20state%20of%20the%20kappa%20opioid%20receptor&journal=Cell&doi=10.1016%2Fj.cell.2017.12.011&volume=172&pages=55-67&publication_year=2018&author=Che%2CT"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="9."><p class="c-article-references__text" id="ref-CR9">Che, T., Dwivedi-Agnihotri, H., Shukla, A. K. & Roth, B. L. Biased ligands at opioid receptors: current status and future directions. <i>Sci. Signal.</i> <b>14</b>, aav0320 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1126/scisignal.aav0320" data-track-item_id="10.1126/scisignal.aav0320" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1126%2Fscisignal.aav0320" aria-label="Article reference 9" data-doi="10.1126/scisignal.aav0320">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 9" href="http://scholar.google.com/scholar_lookup?&title=Biased%20ligands%20at%20opioid%20receptors%3A%20current%20status%20and%20future%20directions&journal=Sci.%20Signal.&doi=10.1126%2Fscisignal.aav0320&volume=14&publication_year=2021&author=Che%2CT&author=Dwivedi-Agnihotri%2CH&author=Shukla%2CAK&author=Roth%2CBL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="10."><p class="c-article-references__text" id="ref-CR10">Liu-Chen, L. Y. & Huang, P. Signaling underlying kappa opioid receptor-mediated behaviors in rodents. <i>Front. Neurosci.</i> <b>16</b>, 964724 (2022).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3389/fnins.2022.964724" data-track-item_id="10.3389/fnins.2022.964724" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3389%2Ffnins.2022.964724" aria-label="Article reference 10" data-doi="10.3389/fnins.2022.964724">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=36408401" aria-label="PubMed reference 10">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9670127" aria-label="PubMed Central reference 10">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 10" href="http://scholar.google.com/scholar_lookup?&title=Signaling%20underlying%20kappa%20opioid%20receptor-mediated%20behaviors%20in%20rodents&journal=Front.%20Neurosci.&doi=10.3389%2Ffnins.2022.964724&volume=16&publication_year=2022&author=Liu-Chen%2CLY&author=Huang%2CP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="11."><p class="c-article-references__text" id="ref-CR11">Machelska, H. & Celik, M. O. Advances in achieving opioid analgesia without side effects. <i>Front. Pharmacol.</i> <b>9</b>, 1388 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3389/fphar.2018.01388" data-track-item_id="10.3389/fphar.2018.01388" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3389%2Ffphar.2018.01388" aria-label="Article reference 11" data-doi="10.3389/fphar.2018.01388">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1MXhtVGmurjM" aria-label="CAS reference 11">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=30555325" aria-label="PubMed reference 11">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282113" aria-label="PubMed Central reference 11">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 11" href="http://scholar.google.com/scholar_lookup?&title=Advances%20in%20achieving%20opioid%20analgesia%20without%20side%20effects&journal=Front.%20Pharmacol.&doi=10.3389%2Ffphar.2018.01388&volume=9&publication_year=2018&author=Machelska%2CH&author=Celik%2CMO"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="12."><p class="c-article-references__text" id="ref-CR12">Dalefield, M. L., Scouller, B., Bibi, R. & Kivell, B. M. The kappa opioid receptor: a promising therapeutic target for multiple pathologies. <i>Front. Pharmacol.</i> <b>13</b>, 837671 (2022).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3389/fphar.2022.837671" data-track-item_id="10.3389/fphar.2022.837671" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3389%2Ffphar.2022.837671" aria-label="Article reference 12" data-doi="10.3389/fphar.2022.837671">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB38Xhsl2ksL3K" aria-label="CAS reference 12">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=35795569" aria-label="PubMed reference 12">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251383" aria-label="PubMed Central reference 12">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 12" href="http://scholar.google.com/scholar_lookup?&title=The%20kappa%20opioid%20receptor%3A%20a%20promising%20therapeutic%20target%20for%20multiple%20pathologies&journal=Front.%20Pharmacol.&doi=10.3389%2Ffphar.2022.837671&volume=13&publication_year=2022&author=Dalefield%2CML&author=Scouller%2CB&author=Bibi%2CR&author=Kivell%2CBM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="13."><p class="c-article-references__text" id="ref-CR13">Bachmutsky, I., Wei, X. P., Durand, A. & Yackle, K. β-arrestin 2 germline knockout does not attenuate opioid respiratory depression. <i>Elife</i> <b>10</b>, <a href="https://doi.org/10.7554/eLife.62552" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.7554/eLife.62552">https://doi.org/10.7554/eLife.62552</a> (2021).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="14."><p class="c-article-references__text" id="ref-CR14">Kliewer, A. et al. Phosphorylation-deficient G-protein-biased mu-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. <i>Nat. Commun.</i> <b>10</b>, 367 (2019).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41467-018-08162-1" data-track-item_id="10.1038/s41467-018-08162-1" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41467-018-08162-1" aria-label="Article reference 14" data-doi="10.1038/s41467-018-08162-1">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2019NatCo..10..367K" aria-label="ADS reference 14">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1MXntFeks7k%3D" aria-label="CAS reference 14">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=30664663" aria-label="PubMed reference 14">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341117" aria-label="PubMed Central reference 14">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 14" href="http://scholar.google.com/scholar_lookup?&title=Phosphorylation-deficient%20G-protein-biased%20mu-opioid%20receptors%20improve%20analgesia%20and%20diminish%20tolerance%20but%20worsen%20opioid%20side%20effects&journal=Nat.%20Commun.&doi=10.1038%2Fs41467-018-08162-1&volume=10&publication_year=2019&author=Kliewer%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="15."><p class="c-article-references__text" id="ref-CR15">Frankowski, K. J. et al. Discovery of small molecule kappa opioid receptor agonist and antagonist chemotypes through a HTS and hit refinement strategy. <i>ACS Chem. Neurosci.</i> <b>3</b>, 221–236 (2012).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/cn200128x" data-track-item_id="10.1021/cn200128x" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Fcn200128x" aria-label="Article reference 15" data-doi="10.1021/cn200128x">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC38XpslGgug%3D%3D" aria-label="CAS reference 15">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=22737280" aria-label="PubMed reference 15">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378255" aria-label="PubMed Central reference 15">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 15" href="http://scholar.google.com/scholar_lookup?&title=Discovery%20of%20small%20molecule%20kappa%20opioid%20receptor%20agonist%20and%20antagonist%20chemotypes%20through%20a%20HTS%20and%20hit%20refinement%20strategy&journal=ACS%20Chem.%20Neurosci.&doi=10.1021%2Fcn200128x&volume=3&pages=221-236&publication_year=2012&author=Frankowski%2CKJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="16."><p class="c-article-references__text" id="ref-CR16">Negri, A. et al. Discovery of a novel selective kappa-opioid receptor agonist using crystal structure-based virtual screening. <i>J. Chem. Inf. Model</i> <b>53</b>, 521–526 (2013).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/ci400019t" data-track-item_id="10.1021/ci400019t" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Fci400019t" aria-label="Article reference 16" data-doi="10.1021/ci400019t">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3sXjsVymurk%3D" aria-label="CAS reference 16">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=23461591" aria-label="PubMed reference 16">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702663" aria-label="PubMed Central reference 16">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 16" href="http://scholar.google.com/scholar_lookup?&title=Discovery%20of%20a%20novel%20selective%20kappa-opioid%20receptor%20agonist%20using%20crystal%20structure-based%20virtual%20screening&journal=J.%20Chem.%20Inf.%20Model&doi=10.1021%2Fci400019t&volume=53&pages=521-526&publication_year=2013&author=Negri%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="17."><p class="c-article-references__text" id="ref-CR17">Roth, B. L. et al. Salvinorin A: a potent naturally occurring nonnitrogenous kappa opioid selective agonist. <i>Proc. Natl Acad. Sci. USA</i> <b>99</b>, 11934–11939 (2002).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1073/pnas.182234399" data-track-item_id="10.1073/pnas.182234399" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1073%2Fpnas.182234399" aria-label="Article reference 17" data-doi="10.1073/pnas.182234399">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2002PNAS...9911934R" aria-label="ADS reference 17">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BD38XntFWqtbY%3D" aria-label="CAS reference 17">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=12192085" aria-label="PubMed reference 17">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC129372" aria-label="PubMed Central reference 17">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 17" href="http://scholar.google.com/scholar_lookup?&title=Salvinorin%20A%3A%20a%20potent%20naturally%20occurring%20nonnitrogenous%20kappa%20opioid%20selective%20agonist&journal=Proc.%20Natl%20Acad.%20Sci.%20USA&doi=10.1073%2Fpnas.182234399&volume=99&pages=11934-11939&publication_year=2002&author=Roth%2CBL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="18."><p class="c-article-references__text" id="ref-CR18">Bhardwaj, G. et al. Accurate de novo design of hyperstable constrained peptides. <i>Nature</i> <b>538</b>, 329–335 (2016).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nature19791" data-track-item_id="10.1038/nature19791" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fnature19791" aria-label="Article reference 18" data-doi="10.1038/nature19791">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2016Natur.538..329B" aria-label="ADS reference 18">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC28XhsFWiurnL" aria-label="CAS reference 18">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=27626386" aria-label="PubMed reference 18">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5161715" aria-label="PubMed Central reference 18">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 18" href="http://scholar.google.com/scholar_lookup?&title=Accurate%20de%20novo%20design%20of%20hyperstable%20constrained%20peptides&journal=Nature&doi=10.1038%2Fnature19791&volume=538&pages=329-335&publication_year=2016&author=Bhardwaj%2CG"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="19."><p class="c-article-references__text" id="ref-CR19">Cao, L. et al. De novo design of picomolar SARS-CoV-2 miniprotein inhibitors. <i>Science</i> <b>370</b>, 426–431 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1126/science.abd9909" data-track-item_id="10.1126/science.abd9909" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1126%2Fscience.abd9909" aria-label="Article reference 19" data-doi="10.1126/science.abd9909">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2020Sci...370..426C" aria-label="ADS reference 19">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXitFyqt77I" aria-label="CAS reference 19">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32907861" aria-label="PubMed reference 19">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857403" aria-label="PubMed Central reference 19">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 19" href="http://scholar.google.com/scholar_lookup?&title=De%20novo%20design%20of%20picomolar%20SARS-CoV-2%20miniprotein%20inhibitors&journal=Science&doi=10.1126%2Fscience.abd9909&volume=370&pages=426-431&publication_year=2020&author=Cao%2CL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="20."><p class="c-article-references__text" id="ref-CR20">Chevalier, A. et al. Massively parallel de novo protein design for targeted therapeutics. <i>Nature</i> <b>550</b>, 74–79 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nature23912" data-track-item_id="10.1038/nature23912" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fnature23912" aria-label="Article reference 20" data-doi="10.1038/nature23912">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2017Natur.550...74C" aria-label="ADS reference 20">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXhsFKmsL%2FL" aria-label="CAS reference 20">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=28953867" aria-label="PubMed reference 20">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802399" aria-label="PubMed Central reference 20">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 20" href="http://scholar.google.com/scholar_lookup?&title=Massively%20parallel%20de%20novo%20protein%20design%20for%20targeted%20therapeutics&journal=Nature&doi=10.1038%2Fnature23912&volume=550&pages=74-79&publication_year=2017&author=Chevalier%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="21."><p class="c-article-references__text" id="ref-CR21">Hosseinzadeh, P. et al. Comprehensive computational design of ordered peptide macrocycles. <i>Science</i> <b>358</b>, 1461–1466 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1126/science.aap7577" data-track-item_id="10.1126/science.aap7577" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1126%2Fscience.aap7577" aria-label="Article reference 21" data-doi="10.1126/science.aap7577">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2017Sci...358.1461H" aria-label="ADS reference 21">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXhvFGmtr3I" aria-label="CAS reference 21">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=29242347" aria-label="PubMed reference 21">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5860875" aria-label="PubMed Central reference 21">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 21" href="http://scholar.google.com/scholar_lookup?&title=Comprehensive%20computational%20design%20of%20ordered%20peptide%20macrocycles&journal=Science&doi=10.1126%2Fscience.aap7577&volume=358&pages=1461-1466&publication_year=2017&author=Hosseinzadeh%2CP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="22."><p class="c-article-references__text" id="ref-CR22">Hosseinzadeh, P. et al. Anchor extension: a structure-guided approach to design cyclic peptides targeting enzyme active sites. <i>Nat. Commun.</i> <b>12</b>, 3384 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41467-021-23609-8" data-track-item_id="10.1038/s41467-021-23609-8" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41467-021-23609-8" aria-label="Article reference 22" data-doi="10.1038/s41467-021-23609-8">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2021NatCo..12.3384H" aria-label="ADS reference 22">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXhsVOqtrrP" aria-label="CAS reference 22">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=34099674" aria-label="PubMed reference 22">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185074" aria-label="PubMed Central reference 22">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 22" href="http://scholar.google.com/scholar_lookup?&title=Anchor%20extension%3A%20a%20structure-guided%20approach%20to%20design%20cyclic%20peptides%20targeting%20enzyme%20active%20sites&journal=Nat.%20Commun.&doi=10.1038%2Fs41467-021-23609-8&volume=12&publication_year=2021&author=Hosseinzadeh%2CP"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="23."><p class="c-article-references__text" id="ref-CR23">Mulligan, V. K. et al. Computationally designed peptide macrocycle inhibitors of New Delhi metallo-beta-lactamase 1. <i>Proc. Natl Acad. Sci. USA</i> <b>118</b>, e2012800118 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1073/pnas.2012800118" data-track-item_id="10.1073/pnas.2012800118" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1073%2Fpnas.2012800118" aria-label="Article reference 23" data-doi="10.1073/pnas.2012800118">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXnt1eltr4%3D" aria-label="CAS reference 23">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=33723038" aria-label="PubMed reference 23">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000195" aria-label="PubMed Central reference 23">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 23" href="http://scholar.google.com/scholar_lookup?&title=Computationally%20designed%20peptide%20macrocycle%20inhibitors%20of%20New%20Delhi%20metallo-beta-lactamase%201&journal=Proc.%20Natl%20Acad.%20Sci.%20USA&doi=10.1073%2Fpnas.2012800118&volume=118&publication_year=2021&author=Mulligan%2CVK"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="24."><p class="c-article-references__text" id="ref-CR24">Danev, R. et al. Routine sub-2.5 Å cryo-EM structure determination of GPCRs. <i>Nat. Commun.</i> <b>12</b>, 4333 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41467-021-24650-3" data-track-item_id="10.1038/s41467-021-24650-3" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41467-021-24650-3" aria-label="Article reference 24" data-doi="10.1038/s41467-021-24650-3">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2021NatCo..12.4333D" aria-label="ADS reference 24">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXhsFOntbbO" aria-label="CAS reference 24">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=34267200" aria-label="PubMed reference 24">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282782" aria-label="PubMed Central reference 24">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 24" href="http://scholar.google.com/scholar_lookup?&title=Routine%20sub-2.5%20%C3%85%20cryo-EM%20structure%20determination%20of%20GPCRs&journal=Nat.%20Commun.&doi=10.1038%2Fs41467-021-24650-3&volume=12&publication_year=2021&author=Danev%2CR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="25."><p class="c-article-references__text" id="ref-CR25">Hedderich, J. B. et al. The pocketome of G protein-coupled receptors reveals previously untargeted allosteric sites. <i>Nat. Commun.</i> <b>13</b>, 2567 (2022).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41467-022-29609-6" data-track-item_id="10.1038/s41467-022-29609-6" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41467-022-29609-6" aria-label="Article reference 25" data-doi="10.1038/s41467-022-29609-6">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2022NatCo..13.2567H" aria-label="ADS reference 25">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB38Xht1Oit7bF" aria-label="CAS reference 25">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=35538063" aria-label="PubMed reference 25">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091257" aria-label="PubMed Central reference 25">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 25" href="http://scholar.google.com/scholar_lookup?&title=The%20pocketome%20of%20G%20protein-coupled%20receptors%20reveals%20previously%20untargeted%20allosteric%20sites&journal=Nat.%20Commun.&doi=10.1038%2Fs41467-022-29609-6&volume=13&publication_year=2022&author=Hedderich%2CJB"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="26."><p class="c-article-references__text" id="ref-CR26">Varadi, A. et al. Synthesis and characterization of a dual kappa-delta opioid receptor agonist analgesic blocking cocaine reward behavior. <i>ACS Chem. Neurosci.</i> <b>6</b>, 1813–1824 (2015).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acschemneuro.5b00153" data-track-item_id="10.1021/acschemneuro.5b00153" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facschemneuro.5b00153" aria-label="Article reference 26" data-doi="10.1021/acschemneuro.5b00153">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2MXhsVart7fL" aria-label="CAS reference 26">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=26325040" aria-label="PubMed reference 26">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 26" href="http://scholar.google.com/scholar_lookup?&title=Synthesis%20and%20characterization%20of%20a%20dual%20kappa-delta%20opioid%20receptor%20agonist%20analgesic%20blocking%20cocaine%20reward%20behavior&journal=ACS%20Chem.%20Neurosci.&doi=10.1021%2Facschemneuro.5b00153&volume=6&pages=1813-1824&publication_year=2015&author=Varadi%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="27."><p class="c-article-references__text" id="ref-CR27">Majumdar, S. et al. Synthesis and evaluation of aryl-naloxamide opiate analgesics targeting truncated exon 11-associated mu opioid receptor (MOR-1.) splice variants. <i>J. Med. Chem.</i> <b>55</b>, 6352–6362 (2012).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/jm300305c" data-track-item_id="10.1021/jm300305c" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Fjm300305c" aria-label="Article reference 27" data-doi="10.1021/jm300305c">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC38XptF2nsbw%3D" aria-label="CAS reference 27">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=22734622" aria-label="PubMed reference 27">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412067" aria-label="PubMed Central reference 27">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 27" href="http://scholar.google.com/scholar_lookup?&title=Synthesis%20and%20evaluation%20of%20aryl-naloxamide%20opiate%20analgesics%20targeting%20truncated%20exon%2011-associated%20mu%20opioid%20receptor%20%28MOR-1.%29%20splice%20variants&journal=J.%20Med.%20Chem.&doi=10.1021%2Fjm300305c&volume=55&pages=6352-6362&publication_year=2012&author=Majumdar%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="28."><p class="c-article-references__text" id="ref-CR28">Wang, C. K., Swedberg, J. E., Northfield, S. E. & Craik, D. J. Effects of cyclization on peptide backbone dynamics. <i>J. Phys. Chem. B</i> <b>119</b>, 15821–15830 (2015).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acs.jpcb.5b11085" data-track-item_id="10.1021/acs.jpcb.5b11085" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facs.jpcb.5b11085" aria-label="Article reference 28" data-doi="10.1021/acs.jpcb.5b11085">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2MXhvFGnsr%2FM" aria-label="CAS reference 28">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=26633076" aria-label="PubMed reference 28">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 28" href="http://scholar.google.com/scholar_lookup?&title=Effects%20of%20cyclization%20on%20peptide%20backbone%20dynamics&journal=J.%20Phys.%20Chem.%20B&doi=10.1021%2Facs.jpcb.5b11085&volume=119&pages=15821-15830&publication_year=2015&author=Wang%2CCK&author=Swedberg%2CJE&author=Northfield%2CSE&author=Craik%2CDJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="29."><p class="c-article-references__text" id="ref-CR29">Uprety, R. et al. Controlling opioid receptor functional selectivity by targeting distinct subpockets of the orthosteric site. <i>Elife</i> <b>10</b>, <a href="https://doi.org/10.7554/eLife.56519" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.7554/eLife.56519">https://doi.org/10.7554/eLife.56519</a> (2021).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="30."><p class="c-article-references__text" id="ref-CR30">Kelly, E. Efficacy and ligand bias at the mu-opioid receptor. <i>Br. J. Pharmacol.</i> <b>169</b>, 1430–1446 (2013).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1111/bph.12222" data-track-item_id="10.1111/bph.12222" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1111%2Fbph.12222" aria-label="Article reference 30" data-doi="10.1111/bph.12222">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3sXhtFOjs77M" aria-label="CAS reference 30">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=23646826" aria-label="PubMed reference 30">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724102" aria-label="PubMed Central reference 30">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 30" href="http://scholar.google.com/scholar_lookup?&title=Efficacy%20and%20ligand%20bias%20at%20the%20mu-opioid%20receptor&journal=Br.%20J.%20Pharmacol.&doi=10.1111%2Fbph.12222&volume=169&pages=1430-1446&publication_year=2013&author=Kelly%2CE"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="31."><p class="c-article-references__text" id="ref-CR31">Brust, T. F. et al. Biased agonists of the kappa opioid receptor suppress pain and itch without causing sedation or dysphoria. <i>Sci. Signal.</i> <b>9</b>, aai8441 (2016).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1126/scisignal.aai8441" data-track-item_id="10.1126/scisignal.aai8441" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1126%2Fscisignal.aai8441" aria-label="Article reference 31" data-doi="10.1126/scisignal.aai8441">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 31" href="http://scholar.google.com/scholar_lookup?&title=Biased%20agonists%20of%20the%20kappa%20opioid%20receptor%20suppress%20pain%20and%20itch%20without%20causing%20sedation%20or%20dysphoria&journal=Sci.%20Signal.&doi=10.1126%2Fscisignal.aai8441&volume=9&publication_year=2016&author=Brust%2CTF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="32."><p class="c-article-references__text" id="ref-CR32">Faouzi, A., Varga, B. R. & Majumdar, S. Biased opioid ligands. <i>Molecules</i> <b>25</b>, <a href="https://doi.org/10.3390/molecules25184257" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.3390/molecules25184257">https://doi.org/10.3390/molecules25184257</a> (2020).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="33."><p class="c-article-references__text" id="ref-CR33">Bohn, L. M. & Aube, J. Seeking (and Finding) biased ligands of the kappa opioid receptor. <i>ACS Med. Chem. Lett.</i> <b>8</b>, 694–700 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acsmedchemlett.7b00224" data-track-item_id="10.1021/acsmedchemlett.7b00224" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facsmedchemlett.7b00224" aria-label="Article reference 33" data-doi="10.1021/acsmedchemlett.7b00224">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXhtV2qu7vI" aria-label="CAS reference 33">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=28740600" aria-label="PubMed reference 33">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5512133" aria-label="PubMed Central reference 33">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 33" href="http://scholar.google.com/scholar_lookup?&title=Seeking%20%28and%20Finding%29%20biased%20ligands%20of%20the%20kappa%20opioid%20receptor&journal=ACS%20Med.%20Chem.%20Lett.&doi=10.1021%2Facsmedchemlett.7b00224&volume=8&pages=694-700&publication_year=2017&author=Bohn%2CLM&author=Aube%2CJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="34."><p class="c-article-references__text" id="ref-CR34">Paton, K. F., Atigari, D. V., Kaska, S., Prisinzano, T. & Kivell, B. M. Strategies for developing kappa opioid receptor agonists for the treatment of pain with fewer side. <i>Effects. J. Pharmacol. Exp. Ther.</i> <b>375</b>, 332–348 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1124/jpet.120.000134" data-track-item_id="10.1124/jpet.120.000134" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1124%2Fjpet.120.000134" aria-label="Article reference 34" data-doi="10.1124/jpet.120.000134">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXit1GlsrrL" aria-label="CAS reference 34">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32913006" aria-label="PubMed reference 34">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 34" href="http://scholar.google.com/scholar_lookup?&title=Strategies%20for%20developing%20kappa%20opioid%20receptor%20agonists%20for%20the%20treatment%20of%20pain%20with%20fewer%20side&journal=Effects.%20J.%20Pharmacol.%20Exp.%20Ther.&doi=10.1124%2Fjpet.120.000134&volume=375&pages=332-348&publication_year=2020&author=Paton%2CKF&author=Atigari%2CDV&author=Kaska%2CS&author=Prisinzano%2CT&author=Kivell%2CBM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="35."><p class="c-article-references__text" id="ref-CR35">Olsen, R. H. J. et al. TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome. <i>Nat. Chem. Biol.</i> <b>16</b>, 841–849 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41589-020-0535-8" data-track-item_id="10.1038/s41589-020-0535-8" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41589-020-0535-8" aria-label="Article reference 35" data-doi="10.1038/s41589-020-0535-8">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXosV2js7o%3D" aria-label="CAS reference 35">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32367019" aria-label="PubMed reference 35">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648517" aria-label="PubMed Central reference 35">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 35" href="http://scholar.google.com/scholar_lookup?&title=TRUPATH%2C%20an%20open-source%20biosensor%20platform%20for%20interrogating%20the%20GPCR%20transducerome&journal=Nat.%20Chem.%20Biol.&doi=10.1038%2Fs41589-020-0535-8&volume=16&pages=841-849&publication_year=2020&author=Olsen%2CRHJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="36."><p class="c-article-references__text" id="ref-CR36">Koehl, A. et al. Structure of the micro-opioid receptor-Gi protein complex. <i>Nature</i> <b>558</b>, 547–552 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41586-018-0219-7" data-track-item_id="10.1038/s41586-018-0219-7" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41586-018-0219-7" aria-label="Article reference 36" data-doi="10.1038/s41586-018-0219-7">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2018Natur.558..547K" aria-label="ADS reference 36">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1cXhtFeqtbjP" aria-label="CAS reference 36">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=29899455" aria-label="PubMed reference 36">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317904" aria-label="PubMed Central reference 36">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 36" href="http://scholar.google.com/scholar_lookup?&title=Structure%20of%20the%20micro-opioid%20receptor-Gi%20protein%20complex&journal=Nature&doi=10.1038%2Fs41586-018-0219-7&volume=558&pages=547-552&publication_year=2018&author=Koehl%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="37."><p class="c-article-references__text" id="ref-CR37">Wu, H. et al. Structure of the human kappa-opioid receptor in complex with JDTic. <i>Nature</i> <b>485</b>, 327–332 (2012).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nature10939" data-track-item_id="10.1038/nature10939" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fnature10939" aria-label="Article reference 37" data-doi="10.1038/nature10939">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2012Natur.485..327W" aria-label="ADS reference 37">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC38XksVegt7k%3D" aria-label="CAS reference 37">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=22437504" aria-label="PubMed reference 37">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356457" aria-label="PubMed Central reference 37">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 37" href="http://scholar.google.com/scholar_lookup?&title=Structure%20of%20the%20human%20kappa-opioid%20receptor%20in%20complex%20with%20JDTic&journal=Nature&doi=10.1038%2Fnature10939&volume=485&pages=327-332&publication_year=2012&author=Wu%2CH"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="38."><p class="c-article-references__text" id="ref-CR38">Wang, Y. et al. Structures of the entire human opioid receptor family. <i>Cell</i> <b>186</b>, 413–427, (2023).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.cell.2022.12.026" data-track-item_id="10.1016/j.cell.2022.12.026" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2Fj.cell.2022.12.026" aria-label="Article reference 38" data-doi="10.1016/j.cell.2022.12.026">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3sXhtVGns7g%3D" aria-label="CAS reference 38">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=36638794" aria-label="PubMed reference 38">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 38" href="http://scholar.google.com/scholar_lookup?&title=Structures%20of%20the%20entire%20human%20opioid%20receptor%20family&journal=Cell&doi=10.1016%2Fj.cell.2022.12.026&volume=186&pages=413-427%2C&publication_year=2023&author=Wang%2CY"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="39."><p class="c-article-references__text" id="ref-CR39">Fink, E. A. et al. Structure-based discovery of nonopioid analgesics acting through the alpha(2A)-adrenergic receptor. <i>Science</i> <b>377</b>, eabn7065 (2022).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1126/science.abn7065" data-track-item_id="10.1126/science.abn7065" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1126%2Fscience.abn7065" aria-label="Article reference 39" data-doi="10.1126/science.abn7065">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB38XisFOru7%2FE" aria-label="CAS reference 39">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=36173843" aria-label="PubMed reference 39">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10360211" aria-label="PubMed Central reference 39">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 39" href="http://scholar.google.com/scholar_lookup?&title=Structure-based%20discovery%20of%20nonopioid%20analgesics%20acting%20through%20the%20alpha%282A%29-adrenergic%20receptor&journal=Science&doi=10.1126%2Fscience.abn7065&volume=377&publication_year=2022&author=Fink%2CEA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="40."><p class="c-article-references__text" id="ref-CR40">Bedini, A. et al. Functional selectivity and antinociceptive effects of a novel KOPr agonist. <i>Front. Pharmacol.</i> <b>11</b>, 188 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3389/fphar.2020.00188" data-track-item_id="10.3389/fphar.2020.00188" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3389%2Ffphar.2020.00188" aria-label="Article reference 40" data-doi="10.3389/fphar.2020.00188">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXhvVarsbvJ" aria-label="CAS reference 40">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32210803" aria-label="PubMed reference 40">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066533" aria-label="PubMed Central reference 40">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 40" href="http://scholar.google.com/scholar_lookup?&title=Functional%20selectivity%20and%20antinociceptive%20effects%20of%20a%20novel%20KOPr%20agonist&journal=Front.%20Pharmacol.&doi=10.3389%2Ffphar.2020.00188&volume=11&publication_year=2020&author=Bedini%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="41."><p class="c-article-references__text" id="ref-CR41">Spetea, M. et al. Selective kappa receptor partial agonist HS666 produces potent antinociception without inducing aversion after i.c.v. administration in mice. <i>Br. J. Pharmacol.</i> <b>174</b>, 2444–2456 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1111/bph.13854" data-track-item_id="10.1111/bph.13854" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1111%2Fbph.13854" aria-label="Article reference 41" data-doi="10.1111/bph.13854">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXhtVKhs7rL" aria-label="CAS reference 41">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=28494108" aria-label="PubMed reference 41">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513865" aria-label="PubMed Central reference 41">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 41" href="http://scholar.google.com/scholar_lookup?&title=Selective%20kappa%20receptor%20partial%20agonist%20HS666%20produces%20potent%20antinociception%20without%20inducing%20aversion%20after%20i.c.v.%20administration%20in%20mice&journal=Br.%20J.%20Pharmacol.&doi=10.1111%2Fbph.13854&volume=174&pages=2444-2456&publication_year=2017&author=Spetea%2CM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="42."><p class="c-article-references__text" id="ref-CR42">Spetea, M. & Schmidhammer, H. Recent chemical and pharmacological developments on 14-oxygenated-N-methylmorphinan-6-ones. <i>Molecules</i> <b>26</b>, <a href="https://doi.org/10.3390/molecules26185677" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.3390/molecules26185677">https://doi.org/10.3390/molecules26185677</a> (2021).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="43."><p class="c-article-references__text" id="ref-CR43">McCorvy, J. D. et al. Structure-inspired design of beta-arrestin-biased ligands for aminergic GPCRs. <i>Nat. Chem. Biol.</i> <b>14</b>, 126–134 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nchembio.2527" data-track-item_id="10.1038/nchembio.2527" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fnchembio.2527" aria-label="Article reference 43" data-doi="10.1038/nchembio.2527">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXhvFGmur3I" aria-label="CAS reference 43">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=29227473" aria-label="PubMed reference 43">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 43" href="http://scholar.google.com/scholar_lookup?&title=Structure-inspired%20design%20of%20beta-arrestin-biased%20ligands%20for%20aminergic%20GPCRs&journal=Nat.%20Chem.%20Biol.&doi=10.1038%2Fnchembio.2527&volume=14&pages=126-134&publication_year=2018&author=McCorvy%2CJD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="44."><p class="c-article-references__text" id="ref-CR44">Chun, L. S. et al. Structure-activity investigation of a G protein-biased agonist reveals molecular determinants for biased signaling of the D2 dopamine receptor. <i>Front. Synaptic Neurosci.</i> <b>10</b>, 2 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3389/fnsyn.2018.00002" data-track-item_id="10.3389/fnsyn.2018.00002" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3389%2Ffnsyn.2018.00002" aria-label="Article reference 44" data-doi="10.3389/fnsyn.2018.00002">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=29515433" aria-label="PubMed reference 44">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5826336" aria-label="PubMed Central reference 44">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 44" href="http://scholar.google.com/scholar_lookup?&title=Structure-activity%20investigation%20of%20a%20G%20protein-biased%20agonist%20reveals%20molecular%20determinants%20for%20biased%20signaling%20of%20the%20D2%20dopamine%20receptor&journal=Front.%20Synaptic%20Neurosci.&doi=10.3389%2Ffnsyn.2018.00002&volume=10&publication_year=2018&author=Chun%2CLS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="45."><p class="c-article-references__text" id="ref-CR45">Yang, D. et al. G protein-coupled receptors: structure- and function-based drug discovery. <i>Signal. Transduct. Target Ther.</i> <b>6</b>, 7 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41392-020-00435-w" data-track-item_id="10.1038/s41392-020-00435-w" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41392-020-00435-w" aria-label="Article reference 45" data-doi="10.1038/s41392-020-00435-w">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXis1Kjtr4%3D" aria-label="CAS reference 45">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=33414387" aria-label="PubMed reference 45">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790836" aria-label="PubMed Central reference 45">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 45" href="http://scholar.google.com/scholar_lookup?&title=G%20protein-coupled%20receptors%3A%20structure-%20and%20function-based%20drug%20discovery&journal=Signal.%20Transduct.%20Target%20Ther.&doi=10.1038%2Fs41392-020-00435-w&volume=6&publication_year=2021&author=Yang%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="46."><p class="c-article-references__text" id="ref-CR46">O’Boyle, N. M. et al. Open Babel: an open chemical toolbox. <i>J. Cheminform.</i> <b>3</b>, 33 (2011).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="noopener" data-track-label="10.1186/1758-2946-3-33" data-track-item_id="10.1186/1758-2946-3-33" data-track-value="article reference" data-track-action="article reference" href="https://link.springer.com/doi/10.1186/1758-2946-3-33" aria-label="Article reference 46" data-doi="10.1186/1758-2946-3-33">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=21982300" aria-label="PubMed reference 46">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198950" aria-label="PubMed Central reference 46">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 46" href="http://scholar.google.com/scholar_lookup?&title=Open%20Babel%3A%20an%20open%20chemical%20toolbox&journal=J.%20Cheminform.&doi=10.1186%2F1758-2946-3-33&volume=3&publication_year=2011&author=O%E2%80%99Boyle%2CNM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="47."><p class="c-article-references__text" id="ref-CR47">Coutsias, E. A., Seok, C., Jacobson, M. P. & Dill, K. A. A kinematic view of loop closure. <i>J. Comput. Chem.</i> <b>25</b>, 510–528 (2004).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1002/jcc.10416" data-track-item_id="10.1002/jcc.10416" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1002%2Fjcc.10416" aria-label="Article reference 47" data-doi="10.1002/jcc.10416">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BD2cXhvVOjtLs%3D" aria-label="CAS reference 47">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=14735570" aria-label="PubMed reference 47">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 47" href="http://scholar.google.com/scholar_lookup?&title=A%20kinematic%20view%20of%20loop%20closure&journal=J.%20Comput.%20Chem.&doi=10.1002%2Fjcc.10416&volume=25&pages=510-528&publication_year=2004&author=Coutsias%2CEA&author=Seok%2CC&author=Jacobson%2CMP&author=Dill%2CKA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="48."><p class="c-article-references__text" id="ref-CR48">Mandell, D. J., Coutsias, E. A. & Kortemme, T. Sub-angstrom accuracy in protein loop reconstruction by tics-inspired conformational sampling. <i>Nat. Methods</i> <b>6</b>, 551–552 (2009).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nmeth0809-551" data-track-item_id="10.1038/nmeth0809-551" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fnmeth0809-551" aria-label="Article reference 48" data-doi="10.1038/nmeth0809-551">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BD1MXpt1Whtbw%3D" aria-label="CAS reference 48">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=19644455" aria-label="PubMed reference 48">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847683" aria-label="PubMed Central reference 48">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 48" href="http://scholar.google.com/scholar_lookup?&title=Sub-angstrom%20accuracy%20in%20protein%20loop%20reconstruction%20by%20tics-inspired%20conformational%20sampling&journal=Nat.%20Methods&doi=10.1038%2Fnmeth0809-551&volume=6&pages=551-552&publication_year=2009&author=Mandell%2CDJ&author=Coutsias%2CEA&author=Kortemme%2CT"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="49."><p class="c-article-references__text" id="ref-CR49">Tyka, M. D. et al. Alternate states of proteins revealed by detailed energy landscape mapping. <i>J. Mol. Biol.</i> <b>405</b>, 607–618 (2011).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.jmb.2010.11.008" data-track-item_id="10.1016/j.jmb.2010.11.008" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2Fj.jmb.2010.11.008" aria-label="Article reference 49" data-doi="10.1016/j.jmb.2010.11.008">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3MXktVWjsg%3D%3D" aria-label="CAS reference 49">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=21073878" aria-label="PubMed reference 49">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 49" href="http://scholar.google.com/scholar_lookup?&title=Alternate%20states%20of%20proteins%20revealed%20by%20detailed%20energy%20landscape%20mapping&journal=J.%20Mol.%20Biol.&doi=10.1016%2Fj.jmb.2010.11.008&volume=405&pages=607-618&publication_year=2011&author=Tyka%2CMD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="50."><p class="c-article-references__text" id="ref-CR50">Cheneval, O. et al. Fmoc-based synthesis of disulfide-rich cyclic peptides. <i>J. Org. Chem.</i> <b>79</b>, 5538–5544 (2014).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/jo500699m" data-track-item_id="10.1021/jo500699m" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Fjo500699m" aria-label="Article reference 50" data-doi="10.1021/jo500699m">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2cXos1Wksrw%3D" aria-label="CAS reference 50">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=24918986" aria-label="PubMed reference 50">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 50" href="http://scholar.google.com/scholar_lookup?&title=Fmoc-based%20synthesis%20of%20disulfide-rich%20cyclic%20peptides&journal=J.%20Org.%20Chem.&doi=10.1021%2Fjo500699m&volume=79&pages=5538-5544&publication_year=2014&author=Cheneval%2CO"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="51."><p class="c-article-references__text" id="ref-CR51">Koehbach, J. et al. Oxytocic plant cyclotides as templates for peptide G protein-coupled receptor ligand design. <i>Proc. Natl Acad. Sci. USA</i> <b>110</b>, 21183–21188 (2013).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1073/pnas.1311183110" data-track-item_id="10.1073/pnas.1311183110" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1073%2Fpnas.1311183110" aria-label="Article reference 51" data-doi="10.1073/pnas.1311183110">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2013PNAS..11021183K" aria-label="ADS reference 51">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2cXnsF2rtg%3D%3D" aria-label="CAS reference 51">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=24248349" aria-label="PubMed reference 51">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876230" aria-label="PubMed Central reference 51">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 51" href="http://scholar.google.com/scholar_lookup?&title=Oxytocic%20plant%20cyclotides%20as%20templates%20for%20peptide%20G%20protein-coupled%20receptor%20ligand%20design&journal=Proc.%20Natl%20Acad.%20Sci.%20USA&doi=10.1073%2Fpnas.1311183110&volume=110&pages=21183-21188&publication_year=2013&author=Koehbach%2CJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="52."><p class="c-article-references__text" id="ref-CR52">Dumitrascuta, M. et al. Antinociceptive efficacy of the micro-opioid/nociceptin peptide-based Hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking. <i>Molecules</i> <b>26</b>, <a href="https://doi.org/10.3390/molecules26113267" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.3390/molecules26113267">https://doi.org/10.3390/molecules26113267</a> (2021).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="53."><p class="c-article-references__text" id="ref-CR53">Erli, F. et al. Highly potent and selective new diphenethylamines interacting with the kappa-opioid receptor: synthesis, pharmacology, and structure-activity relationships. <i>J. Med. Chem.</i> <b>60</b>, 7579–7590 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acs.jmedchem.7b00981" data-track-item_id="10.1021/acs.jmedchem.7b00981" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facs.jmedchem.7b00981" aria-label="Article reference 53" data-doi="10.1021/acs.jmedchem.7b00981">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXhtlKhur3I" aria-label="CAS reference 53">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=28825813" aria-label="PubMed reference 53">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601360" aria-label="PubMed Central reference 53">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 53" href="http://scholar.google.com/scholar_lookup?&title=Highly%20potent%20and%20selective%20new%20diphenethylamines%20interacting%20with%20the%20kappa-opioid%20receptor%3A%20synthesis%2C%20pharmacology%2C%20and%20structure-activity%20relationships&journal=J.%20Med.%20Chem.&doi=10.1021%2Facs.jmedchem.7b00981&volume=60&pages=7579-7590&publication_year=2017&author=Erli%2CF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="54."><p class="c-article-references__text" id="ref-CR54">Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. <i>Anal. Biochem.</i> <b>72</b>, 248–254 (1976).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/0003-2697(76)90527-3" data-track-item_id="10.1016/0003-2697(76)90527-3" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2F0003-2697%2876%2990527-3" aria-label="Article reference 54" data-doi="10.1016/0003-2697(76)90527-3">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DyaE28XksVehtrY%3D" aria-label="CAS reference 54">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=942051" aria-label="PubMed reference 54">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 54" href="http://scholar.google.com/scholar_lookup?&title=A%20rapid%20and%20sensitive%20method%20for%20the%20quantitation%20of%20microgram%20quantities%20of%20protein%20utilizing%20the%20principle%20of%20protein-dye%20binding&journal=Anal.%20Biochem.&doi=10.1016%2F0003-2697%2876%2990527-3&volume=72&pages=248-254&publication_year=1976&author=Bradford%2CMM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="55."><p class="c-article-references__text" id="ref-CR55">Duerrauer, L. et al. I8-arachnotocin-an arthropod-derived G protein-biased ligand of the human vasopressin V2 receptor. <i>Sci. Rep.</i> <b>9</b>, 19295 (2019).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41598-019-55675-w" data-track-item_id="10.1038/s41598-019-55675-w" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41598-019-55675-w" aria-label="Article reference 55" data-doi="10.1038/s41598-019-55675-w">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2019NatSR...919295D" aria-label="ADS reference 55">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1MXisVGjsbbP" aria-label="CAS reference 55">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=31848378" aria-label="PubMed reference 55">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917733" aria-label="PubMed Central reference 55">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 55" href="http://scholar.google.com/scholar_lookup?&title=I8-arachnotocin-an%20arthropod-derived%20G%20protein-biased%20ligand%20of%20the%20human%20vasopressin%20V2%20receptor&journal=Sci.%20Rep.&doi=10.1038%2Fs41598-019-55675-w&volume=9&publication_year=2019&author=Duerrauer%2CL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="56."><p class="c-article-references__text" id="ref-CR56">Nasrollahi-Shirazi, S. et al. Functional impact of the G279S substitution in the adenosine A(1)-Receptor (A(1)R-G279S(7.44)), a mutation associated with Parkinson’s disease. <i>Mol. Pharmacol.</i> <b>98</b>, 250–266 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1124/molpharm.120.000003" data-track-item_id="10.1124/molpharm.120.000003" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1124%2Fmolpharm.120.000003" aria-label="Article reference 56" data-doi="10.1124/molpharm.120.000003">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXhvVCntr%2FF" aria-label="CAS reference 56">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32817461" aria-label="PubMed reference 56">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 56" href="http://scholar.google.com/scholar_lookup?&title=Functional%20impact%20of%20the%20G279S%20substitution%20in%20the%20adenosine%20A%281%29-Receptor%20%28A%281%29R-G279S%287.44%29%29%2C%20a%20mutation%20associated%20with%20Parkinson%E2%80%99s%20disease&journal=Mol.%20Pharmacol.&doi=10.1124%2Fmolpharm.120.000003&volume=98&pages=250-266&publication_year=2020&author=Nasrollahi-Shirazi%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="57."><p class="c-article-references__text" id="ref-CR57">Nasrollahi-Shirazi, S. et al. Functional impact of the G279S substitution in the adenosine A1-Receptor (A1R-G279S(7.44)), a mutation associated with Parkinson’s disease. <i>Mol. Pharmacol</i>. <b>98</b>, 250–266 (2020).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="58."><p class="c-article-references__text" id="ref-CR58">Muratspahic, E. et al. Design of a stable cyclic peptide analgesic derived from sunflower seeds that targets the kappa-opioid receptor for the treatment of chronic abdominal pain. <i>J. Med. Chem.</i> <b>64</b>, 9042–9055 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acs.jmedchem.1c00158" data-track-item_id="10.1021/acs.jmedchem.1c00158" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facs.jmedchem.1c00158" aria-label="Article reference 58" data-doi="10.1021/acs.jmedchem.1c00158">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXhsVShtbvM" aria-label="CAS reference 58">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=34162205" aria-label="PubMed reference 58">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273886" aria-label="PubMed Central reference 58">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 58" href="http://scholar.google.com/scholar_lookup?&title=Design%20of%20a%20stable%20cyclic%20peptide%20analgesic%20derived%20from%20sunflower%20seeds%20that%20targets%20the%20kappa-opioid%20receptor%20for%20the%20treatment%20of%20chronic%20abdominal%20pain&journal=J.%20Med.%20Chem.&doi=10.1021%2Facs.jmedchem.1c00158&volume=64&pages=9042-9055&publication_year=2021&author=Muratspahic%2CE"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="59."><p class="c-article-references__text" id="ref-CR59">Maeda, S. et al. Development of an antibody fragment that stabilizes GPCR/G-protein complexes. <i>Nat. Commun.</i> <b>9</b>, 3712 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41467-018-06002-w" data-track-item_id="10.1038/s41467-018-06002-w" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41467-018-06002-w" aria-label="Article reference 59" data-doi="10.1038/s41467-018-06002-w">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2018NatCo...9.3712M" aria-label="ADS reference 59">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=30213947" aria-label="PubMed reference 59">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137068" aria-label="PubMed Central reference 59">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 59" href="http://scholar.google.com/scholar_lookup?&title=Development%20of%20an%20antibody%20fragment%20that%20stabilizes%20GPCR%2FG-protein%20complexes&journal=Nat.%20Commun.&doi=10.1038%2Fs41467-018-06002-w&volume=9&publication_year=2018&author=Maeda%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="60."><p class="c-article-references__text" id="ref-CR60">Liang, Y. L. et al. Ominant negative G proteins enhance formation and purification of agonist-GPCR-G protein complexes for structure determination. <i>ACS Pharmacol. Transl. Sci.</i> <b>1</b>, 12–20 (2018).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acsptsci.8b00017" data-track-item_id="10.1021/acsptsci.8b00017" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facsptsci.8b00017" aria-label="Article reference 60" data-doi="10.1021/acsptsci.8b00017">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1cXhtlOktL3E" aria-label="CAS reference 60">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32219201" aria-label="PubMed reference 60">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089020" aria-label="PubMed Central reference 60">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 60" href="http://scholar.google.com/scholar_lookup?&title=Ominant%20negative%20G%20proteins%20enhance%20formation%20and%20purification%20of%20agonist-GPCR-G%20protein%20complexes%20for%20structure%20determination&journal=ACS%20Pharmacol.%20Transl.%20Sci.&doi=10.1021%2Facsptsci.8b00017&volume=1&pages=12-20&publication_year=2018&author=Liang%2CYL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="61."><p class="c-article-references__text" id="ref-CR61">Kim, K. et al. Structure of a hallucinogen-activated Gq-coupled 5-HT2A serotonin receptor. <i>Cell</i> <b>182</b>, 1574–1588 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.cell.2020.08.024" data-track-item_id="10.1016/j.cell.2020.08.024" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2Fj.cell.2020.08.024" aria-label="Article reference 61" data-doi="10.1016/j.cell.2020.08.024">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXhvVymsb3L" aria-label="CAS reference 61">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=32946782" aria-label="PubMed reference 61">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593816" aria-label="PubMed Central reference 61">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 61" href="http://scholar.google.com/scholar_lookup?&title=Structure%20of%20a%20hallucinogen-activated%20Gq-coupled%205-HT2A%20serotonin%20receptor&journal=Cell&doi=10.1016%2Fj.cell.2020.08.024&volume=182&pages=1574-1588&publication_year=2020&author=Kim%2CK"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="62."><p class="c-article-references__text" id="ref-CR62">Peck, J. V., Fay, J. F. & Strauss, J. D. High-speed high-resolution data collection on a 200 keV cryo-TEM. <i>IUCrJ</i> <b>9</b>, 243–252 (2022).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1107/S2052252522000069" data-track-item_id="10.1107/S2052252522000069" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1107%2FS2052252522000069" aria-label="Article reference 62" data-doi="10.1107/S2052252522000069">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB38XmtFOnsLg%3D" aria-label="CAS reference 62">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=35371504" aria-label="PubMed reference 62">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895008" aria-label="PubMed Central reference 62">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 62" href="http://scholar.google.com/scholar_lookup?&title=High-speed%20high-resolution%20data%20collection%20on%20a%20200%20keV%20cryo-TEM&journal=IUCrJ&doi=10.1107%2FS2052252522000069&volume=9&pages=243-252&publication_year=2022&author=Peck%2CJV&author=Fay%2CJF&author=Strauss%2CJD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="63."><p class="c-article-references__text" id="ref-CR63">Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. <i>Nat. Methods</i> <b>14</b>, 290–296 (2017).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/nmeth.4169" data-track-item_id="10.1038/nmeth.4169" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fnmeth.4169" aria-label="Article reference 63" data-doi="10.1038/nmeth.4169">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2sXitlGisbs%3D" aria-label="CAS reference 63">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=28165473" aria-label="PubMed reference 63">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 63" href="http://scholar.google.com/scholar_lookup?&title=A.%20cryoSPARC%3A%20algorithms%20for%20rapid%20unsupervised%20cryo-EM%20structure%20determination&journal=Nat.%20Methods&doi=10.1038%2Fnmeth.4169&volume=14&pages=290-296&publication_year=2017&author=Punjani%2CA&author=Rubinstein%2CJL&author=Fleet%2CDJ&author=Brubaker%2CM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="64."><p class="c-article-references__text" id="ref-CR64">Punjani, A., Zhang, H. & Fleet, D. J. Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction. <i>Nat. Methods</i> <b>17</b>, 1214–1221 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41592-020-00990-8" data-track-item_id="10.1038/s41592-020-00990-8" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41592-020-00990-8" aria-label="Article reference 64" data-doi="10.1038/s41592-020-00990-8">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3cXisVGksbbK" aria-label="CAS reference 64">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=33257830" aria-label="PubMed reference 64">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 64" href="http://scholar.google.com/scholar_lookup?&title=Non-uniform%20refinement%3A%20adaptive%20regularization%20improves%20single-particle%20cryo-EM%20reconstruction&journal=Nat.%20Methods&doi=10.1038%2Fs41592-020-00990-8&volume=17&pages=1214-1221&publication_year=2020&author=Punjani%2CA&author=Zhang%2CH&author=Fleet%2CDJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="65."><p class="c-article-references__text" id="ref-CR65">Cao, C. et al. Structure, function and pharmacology of human itch GPCRs. <i>Nature</i> <b>600</b>, 170–175 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41586-021-04126-6" data-track-item_id="10.1038/s41586-021-04126-6" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41586-021-04126-6" aria-label="Article reference 65" data-doi="10.1038/s41586-021-04126-6">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2021Natur.600..170C" aria-label="ADS reference 65">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXisFWks7rM" aria-label="CAS reference 65">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=34789874" aria-label="PubMed reference 65">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150435" aria-label="PubMed Central reference 65">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 65" href="http://scholar.google.com/scholar_lookup?&title=Structure%2C%20function%20and%20pharmacology%20of%20human%20itch%20GPCRs&journal=Nature&doi=10.1038%2Fs41586-021-04126-6&volume=600&pages=170-175&publication_year=2021&author=Cao%2CC"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="66."><p class="c-article-references__text" id="ref-CR66">Pettersen, E. F. et al. UCSF Chimera–a visualization system for exploratory research and analysis. <i>J. Comput. Chem.</i> <b>25</b>, 1605–1612 (2004).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1002/jcc.20084" data-track-item_id="10.1002/jcc.20084" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1002%2Fjcc.20084" aria-label="Article reference 66" data-doi="10.1002/jcc.20084">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BD2cXmvVOhsbs%3D" aria-label="CAS reference 66">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=15264254" aria-label="PubMed reference 66">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 66" href="http://scholar.google.com/scholar_lookup?&title=UCSF%20Chimera%E2%80%93a%20visualization%20system%20for%20exploratory%20research%20and%20analysis&journal=J.%20Comput.%20Chem.&doi=10.1002%2Fjcc.20084&volume=25&pages=1605-1612&publication_year=2004&author=Pettersen%2CEF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="67."><p class="c-article-references__text" id="ref-CR67">Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. <i>Acta Crystallogr. D Biol. Crystallogr.</i> <b>66</b>, 486–501 (2010).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1107/S0907444910007493" data-track-item_id="10.1107/S0907444910007493" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1107%2FS0907444910007493" aria-label="Article reference 67" data-doi="10.1107/S0907444910007493">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2010AcCrD..66..486E" aria-label="ADS reference 67">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3cXksFKisb8%3D" aria-label="CAS reference 67">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=20383002" aria-label="PubMed reference 67">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852313" aria-label="PubMed Central reference 67">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 67" href="http://scholar.google.com/scholar_lookup?&title=Features%20and%20development%20of%20Coot&journal=Acta%20Crystallogr.%20D%20Biol.%20Crystallogr.&doi=10.1107%2FS0907444910007493&volume=66&pages=486-501&publication_year=2010&author=Emsley%2CP&author=Lohkamp%2CB&author=Scott%2CWG&author=Cowtan%2CK"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="68."><p class="c-article-references__text" id="ref-CR68">Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. <i>Acta Crystallogr. D Biol. Crystallogr.</i> <b>66</b>, 213–221 (2010).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1107/S0907444909052925" data-track-item_id="10.1107/S0907444909052925" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1107%2FS0907444909052925" aria-label="Article reference 68" data-doi="10.1107/S0907444909052925">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2010AcCrD..66..213A" aria-label="ADS reference 68">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3cXhs1Sisbc%3D" aria-label="CAS reference 68">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=20124702" aria-label="PubMed reference 68">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815670" aria-label="PubMed Central reference 68">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 68" href="http://scholar.google.com/scholar_lookup?&title=PHENIX%3A%20a%20comprehensive%20Python-based%20system%20for%20macromolecular%20structure%20solution&journal=Acta%20Crystallogr.%20D%20Biol.%20Crystallogr.&doi=10.1107%2FS0907444909052925&volume=66&pages=213-221&publication_year=2010&author=Adams%2CPD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="69."><p class="c-article-references__text" id="ref-CR69">Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. <i>Acta Crystallogr. D Biol. Crystallogr.</i> <b>66</b>, 12–21 (2010).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1107/S0907444909042073" data-track-item_id="10.1107/S0907444909042073" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1107%2FS0907444909042073" aria-label="Article reference 69" data-doi="10.1107/S0907444909042073">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2010AcCrD..66...12C" aria-label="ADS reference 69">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3cXit1Kktg%3D%3D" aria-label="CAS reference 69">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=20057044" aria-label="PubMed reference 69">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 69" href="http://scholar.google.com/scholar_lookup?&title=MolProbity%3A%20all-atom%20structure%20validation%20for%20macromolecular%20crystallography&journal=Acta%20Crystallogr.%20D%20Biol.%20Crystallogr.&doi=10.1107%2FS0907444909042073&volume=66&pages=12-21&publication_year=2010&author=Chen%2CVB"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="70."><p class="c-article-references__text" id="ref-CR70">Case, D. A. et al. <i>Amber 2023</i> (University of California, 2023).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="71."><p class="c-article-references__text" id="ref-CR71">Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. <i>Nature</i> <b>596</b>, 583–589 (2021).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41586-021-03819-2" data-track-item_id="10.1038/s41586-021-03819-2" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41586-021-03819-2" aria-label="Article reference 71" data-doi="10.1038/s41586-021-03819-2">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2021Natur.596..583J" aria-label="ADS reference 71">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3MXhvVaktrrL" aria-label="CAS reference 71">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=34265844" aria-label="PubMed reference 71">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371605" aria-label="PubMed Central reference 71">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 71" href="http://scholar.google.com/scholar_lookup?&title=Highly%20accurate%20protein%20structure%20prediction%20with%20AlphaFold&journal=Nature&doi=10.1038%2Fs41586-021-03819-2&volume=596&pages=583-589&publication_year=2021&author=Jumper%2CJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="72."><p class="c-article-references__text" id="ref-CR72">Schott-Verdugo, S. & Gohlke, H. PACKMOL-Memgen: a simple-to-use, generalized workflow for membrane-protein-lipid-bilayer system building. <i>J. Chem. Inf. Model</i> <b>59</b>, 2522–2528 (2019).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acs.jcim.9b00269" data-track-item_id="10.1021/acs.jcim.9b00269" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facs.jcim.9b00269" aria-label="Article reference 72" data-doi="10.1021/acs.jcim.9b00269">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC1MXhtVWqtrvI" aria-label="CAS reference 72">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=31120747" aria-label="PubMed reference 72">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 72" href="http://scholar.google.com/scholar_lookup?&title=PACKMOL-Memgen%3A%20a%20simple-to-use%2C%20generalized%20workflow%20for%20membrane-protein-lipid-bilayer%20system%20building&journal=J.%20Chem.%20Inf.%20Model&doi=10.1021%2Facs.jcim.9b00269&volume=59&pages=2522-2528&publication_year=2019&author=Schott-Verdugo%2CS&author=Gohlke%2CH"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="73."><p class="c-article-references__text" id="ref-CR73">Han, J. et al. Ligand and G-protein selectivity in the kappa-opioid receptor. <i>Nature</i> <b>617</b>, 417–425 (2023).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1038/s41586-023-06030-7" data-track-item_id="10.1038/s41586-023-06030-7" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1038%2Fs41586-023-06030-7" aria-label="Article reference 73" data-doi="10.1038/s41586-023-06030-7">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2023Natur.617..417H" aria-label="ADS reference 73">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB3sXpt1arsLs%3D" aria-label="CAS reference 73">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=37138078" aria-label="PubMed reference 73">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172140" aria-label="PubMed Central reference 73">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 73" href="http://scholar.google.com/scholar_lookup?&title=Ligand%20and%20G-protein%20selectivity%20in%20the%20kappa-opioid%20receptor&journal=Nature&doi=10.1038%2Fs41586-023-06030-7&volume=617&pages=417-425&publication_year=2023&author=Han%2CJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="74."><p class="c-article-references__text" id="ref-CR74">Tian, C. et al. ff19SB: amino-acid-specific protein backbone parameters trained against quantum mechanics energy surfaces in solution. <i>J. Chem. Theory Comput.</i> <b>16</b>, 528–552 (2020).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acs.jctc.9b00591" data-track-item_id="10.1021/acs.jctc.9b00591" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facs.jctc.9b00591" aria-label="Article reference 74" data-doi="10.1021/acs.jctc.9b00591">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=31714766" aria-label="PubMed reference 74">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 74" href="http://scholar.google.com/scholar_lookup?&title=ff19SB%3A%20amino-acid-specific%20protein%20backbone%20parameters%20trained%20against%20quantum%20mechanics%20energy%20surfaces%20in%20solution&journal=J.%20Chem.%20Theory%20Comput.&doi=10.1021%2Facs.jctc.9b00591&volume=16&pages=528-552&publication_year=2020&author=Tian%2CC"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="75."><p class="c-article-references__text" id="ref-CR75">Dickson, C. J., Walker, R. C. & Gould, I. R. Lipid21: complex lipid membrane simulations with AMBER. <i>J. Chem. Theory Comput.</i> <b>18</b>, 1726–1736 (2022).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/acs.jctc.1c01217" data-track-item_id="10.1021/acs.jctc.1c01217" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Facs.jctc.1c01217" aria-label="Article reference 75" data-doi="10.1021/acs.jctc.1c01217">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BB38Xis1ymtLg%3D" aria-label="CAS reference 75">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=35113553" aria-label="PubMed reference 75">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9007451" aria-label="PubMed Central reference 75">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 75" href="http://scholar.google.com/scholar_lookup?&title=Lipid21%3A%20complex%20lipid%20membrane%20simulations%20with%20AMBER&journal=J.%20Chem.%20Theory%20Comput.&doi=10.1021%2Facs.jctc.1c01217&volume=18&pages=1726-1736&publication_year=2022&author=Dickson%2CCJ&author=Walker%2CRC&author=Gould%2CIR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="76."><p class="c-article-references__text" id="ref-CR76">Wang, J., Wolf, R. M., Caldwell, J. W., Kollman, P. A. & Case, D. A. Development and testing of a general amber force field. <i>J. Comput. Chem</i> <b>25</b>, 1157–1174 (2004).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1002/jcc.20035" data-track-item_id="10.1002/jcc.20035" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1002%2Fjcc.20035" aria-label="Article reference 76" data-doi="10.1002/jcc.20035">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BD2cXksFakurc%3D" aria-label="CAS reference 76">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=15116359" aria-label="PubMed reference 76">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 76" href="http://scholar.google.com/scholar_lookup?&title=Development%20and%20testing%20of%20a%20general%20amber%20force%20field&journal=J.%20Comput.%20Chem&doi=10.1002%2Fjcc.20035&volume=25&pages=1157-1174&publication_year=2004&author=Wang%2CJ&author=Wolf%2CRM&author=Caldwell%2CJW&author=Kollman%2CPA&author=Case%2CDA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="77."><p class="c-article-references__text" id="ref-CR77">Jorgensen, W. L., Chandrasekhar, J., Madura, J. D., Impey, R. W. & Klein, M. L. Comparison of simple potential functions for simulating liquid water. <i>J. Chem. Phys.</i> <b>79</b>, 926–935 (1983).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1063/1.445869" data-track-item_id="10.1063/1.445869" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1063%2F1.445869" aria-label="Article reference 77" data-doi="10.1063/1.445869">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=1983JChPh..79..926J" aria-label="ADS reference 77">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DyaL3sXksF2htL4%3D" aria-label="CAS reference 77">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 77" href="http://scholar.google.com/scholar_lookup?&title=Comparison%20of%20simple%20potential%20functions%20for%20simulating%20liquid%20water&journal=J.%20Chem.%20Phys.&doi=10.1063%2F1.445869&volume=79&pages=926-935&publication_year=1983&author=Jorgensen%2CWL&author=Chandrasekhar%2CJ&author=Madura%2CJD&author=Impey%2CRW&author=Klein%2CML"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="78."><p class="c-article-references__text" id="ref-CR78">Joung, I. S. & Cheatham, T. E. 3rd Determination of alkali and halide monovalent ion parameters for use in explicitly solvated biomolecular simulations. <i>J. Phys. Chem. B</i> <b>112</b>, 9020–9041 (2008).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/jp8001614" data-track-item_id="10.1021/jp8001614" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Fjp8001614" aria-label="Article reference 78" data-doi="10.1021/jp8001614">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BD1cXnvFGqtL4%3D" aria-label="CAS reference 78">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=18593145" aria-label="PubMed reference 78">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2652252" aria-label="PubMed Central reference 78">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 78" href="http://scholar.google.com/scholar_lookup?&title=Determination%20of%20alkali%20and%20halide%20monovalent%20ion%20parameters%20for%20use%20in%20explicitly%20solvated%20biomolecular%20simulations&journal=J.%20Phys.%20Chem.%20B&doi=10.1021%2Fjp8001614&volume=112&pages=9020-9041&publication_year=2008&author=Joung%2CIS&author=Cheatham%2CTE"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="79."><p class="c-article-references__text" id="ref-CR79">Best, R. B., Hummer, G. & Eaton, W. A. Native contacts determine protein folding mechanisms in atomistic simulations. <i>Proc. Natl Acad. Sci. USA</i> <b>110</b>, 17874–17879 (2013).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1073/pnas.1311599110" data-track-item_id="10.1073/pnas.1311599110" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1073%2Fpnas.1311599110" aria-label="Article reference 79" data-doi="10.1073/pnas.1311599110">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="ads reference" data-track-action="ads reference" href="http://adsabs.harvard.edu/cgi-bin/nph-data_query?link_type=ABSTRACT&bibcode=2013PNAS..11017874B" aria-label="ADS reference 79">ADS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3sXhvVWmur7P" aria-label="CAS reference 79">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=24128758" aria-label="PubMed reference 79">PubMed</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816414" aria-label="PubMed Central reference 79">PubMed Central</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 79" href="http://scholar.google.com/scholar_lookup?&title=Native%20contacts%20determine%20protein%20folding%20mechanisms%20in%20atomistic%20simulations&journal=Proc.%20Natl%20Acad.%20Sci.%20USA&doi=10.1073%2Fpnas.1311599110&volume=110&pages=17874-17879&publication_year=2013&author=Best%2CRB&author=Hummer%2CG&author=Eaton%2CWA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="80."><p class="c-article-references__text" id="ref-CR80">Humphrey, W., Dalke, A. & Schulten, K. VMD: visual molecular dynamics. <i>J.Mol. Graph.</i> <b>14</b>, 27–38 (1996).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/0263-7855(96)00018-5" data-track-item_id="10.1016/0263-7855(96)00018-5" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2F0263-7855%2896%2900018-5" aria-label="Article reference 80" data-doi="10.1016/0263-7855(96)00018-5">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 80" href="http://scholar.google.com/scholar_lookup?&title=VMD%3A%20visual%20molecular%20dynamics&journal=J.Mol.%20Graph.&doi=10.1016%2F0263-7855%2896%2900018-5&volume=14&pages=27-38&publication_year=1996&author=Humphrey%2CW&author=Dalke%2CA&author=Schulten%2CK"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="81."><p class="c-article-references__text" id="ref-CR81">Dubuisson, D. & Dennis, S. G. The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. <i>Pain</i> <b>4</b>, 161–174 (1977).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/0304-3959(77)90130-0" data-track-item_id="10.1016/0304-3959(77)90130-0" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2F0304-3959%2877%2990130-0" aria-label="Article reference 81" data-doi="10.1016/0304-3959(77)90130-0">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DyaE1cXhsVGrt7s%3D" aria-label="CAS reference 81">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=564014" aria-label="PubMed reference 81">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 81" href="http://scholar.google.com/scholar_lookup?&title=The%20formalin%20test%3A%20a%20quantitative%20study%20of%20the%20analgesic%20effects%20of%20morphine%2C%20meperidine%2C%20and%20brain%20stem%20stimulation%20in%20rats%20and%20cats&journal=Pain&doi=10.1016%2F0304-3959%2877%2990130-0&volume=4&pages=161-174&publication_year=1977&author=Dubuisson%2CD&author=Dennis%2CSG"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="82."><p class="c-article-references__text" id="ref-CR82">Puls, K. et al. In vitro, In vivo and In silico Characterization of a Novel Kappa-opioid receptor antagonist. <i>Pharmaceuticals</i> <b>15</b>, <a href="https://doi.org/10.3390/ph15060680" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.3390/ph15060680">https://doi.org/10.3390/ph15060680</a> (2022).</p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="83."><p class="c-article-references__text" id="ref-CR83">Litchfield, J. T. Jr. & Wilcoxon, F. A simplified method of evaluating dose-effect experiments. <i>J. Pharmacol. Exp. Ther.</i> <b>96</b>, 99–113 (1949).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DyaH1MXjvFWmsA%3D%3D" aria-label="CAS reference 83">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=18152921" aria-label="PubMed reference 83">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 83" href="http://scholar.google.com/scholar_lookup?&title=A%20simplified%20method%20of%20evaluating%20dose-effect%20experiments&journal=J.%20Pharmacol.%20Exp.%20Ther.&volume=96&pages=99-113&publication_year=1949&author=Litchfield%2CJT&author=Wilcoxon%2CF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="84."><p class="c-article-references__text" id="ref-CR84">Hargreaves, K., Dubner, R., Brown, F., Flores, C. & Joris, J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. <i>Pain</i> <b>32</b>, 77–88 (1988).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/0304-3959(88)90026-7" data-track-item_id="10.1016/0304-3959(88)90026-7" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2F0304-3959%2888%2990026-7" aria-label="Article reference 84" data-doi="10.1016/0304-3959(88)90026-7">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:STN:280:DyaL1c7itlOntw%3D%3D" aria-label="CAS reference 84">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=3340425" aria-label="PubMed reference 84">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 84" href="http://scholar.google.com/scholar_lookup?&title=A%20new%20and%20sensitive%20method%20for%20measuring%20thermal%20nociception%20in%20cutaneous%20hyperalgesia&journal=Pain&doi=10.1016%2F0304-3959%2888%2990026-7&volume=32&pages=77-88&publication_year=1988&author=Hargreaves%2CK&author=Dubner%2CR&author=Brown%2CF&author=Flores%2CC&author=Joris%2CJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="85."><p class="c-article-references__text" id="ref-CR85">Jones, B. J. & Roberts, D. J. The quantiative measurement of motor inco-ordination in naive mice using an acelerating rotarod. <i>J. Pharm. Pharmacol</i> <b>20</b>, 302–304 (1968).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1111/j.2042-7158.1968.tb09743.x" data-track-item_id="10.1111/j.2042-7158.1968.tb09743.x" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1111%2Fj.2042-7158.1968.tb09743.x" aria-label="Article reference 85" data-doi="10.1111/j.2042-7158.1968.tb09743.x">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:STN:280:DyaF1c7ps1Ogsg%3D%3D" aria-label="CAS reference 85">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=4384609" aria-label="PubMed reference 85">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 85" href="http://scholar.google.com/scholar_lookup?&title=The%20quantiative%20measurement%20of%20motor%20inco-ordination%20in%20naive%20mice%20using%20an%20acelerating%20rotarod&journal=J.%20Pharm.%20Pharmacol&doi=10.1111%2Fj.2042-7158.1968.tb09743.x&volume=20&pages=302-304&publication_year=1968&author=Jones%2CBJ&author=Roberts%2CDJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item" data-counter="86."><p class="c-article-references__text" id="ref-CR86">Cheng, Y. & Prusoff, W. H. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. <i>Biochem. Pharmacol.</i> <b>22</b>, 3099–3108 (1973).</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/0006-2952(73)90196-2" data-track-item_id="10.1016/0006-2952(73)90196-2" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2F0006-2952%2873%2990196-2" aria-label="Article reference 86" data-doi="10.1016/0006-2952(73)90196-2">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DyaE2cXhtVGgs7c%3D" aria-label="CAS reference 86">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=4202581" aria-label="PubMed reference 86">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 86" href="http://scholar.google.com/scholar_lookup?&title=Relationship%20between%20the%20inhibition%20constant%20%28K1%29%20and%20the%20concentration%20of%20inhibitor%20which%20causes%2050%20per%20cent%20inhibition%20%28I50%29%20of%20an%20enzymatic%20reaction&journal=Biochem.%20Pharmacol.&doi=10.1016%2F0006-2952%2873%2990196-2&volume=22&pages=3099-3108&publication_year=1973&author=Cheng%2CY&author=Prusoff%2CWH"> Google Scholar</a> </p></li></ol><p class="c-article-references__download u-hide-print"><a data-track="click" data-track-action="download citation references" data-track-label="link" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1038/s41467-023-43718-w?format=refman&flavour=references">Download references<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></p></div></div></div></section></div><section data-title="Acknowledgements"><div class="c-article-section" id="Ack1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Ack1">Acknowledgements</h2><div class="c-article-section__content" id="Ack1-content"><p>We would like to thank Bryan Roth (UNC, Chapel Hill, NC) for sharing the human KOR plasmid. HEK293T cells stably expressing the mouse MOR and DOR were a kind gift of Dr. Oliver Kudlacek (Medical University of Vienna, Austria). CHO-K1 cells stably expressing the human opioid receptors were kindly provided by Dr. Lawrence Toll (SRI International, Menlo Park, CA). Research of C.W.G. (P32109), M.S. (I4697), R.H. and K.B.J. (ZK-81B) are supported by the Austrian Science Fund (FWF). C.W.G. has been supported by the Austrian Federal Ministry for Labor and Economy and the Federal Ministry for Climate Action, Environment, Energy, Mobility, Innovation and Technology with an AWS Prize project (P2387582). E.M. was a Marietta Blau Fellow of Austrian Federal Ministry of Education, Science and Research (ICM-2019-13441). K.D. and T.C. were Washington Research Foundation Fellows. Research of M.M. was supported by an ERC start grant (FA 705007). Research in the laboratory of D.J.C. is supported by the National Health and Medical Research Council Australia (2009564) and the Australian Research Council (CE200100012).</p></div></div></section><section aria-labelledby="author-information" data-title="Author information"><div class="c-article-section" id="author-information-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="author-information">Author information</h2><div class="c-article-section__content" id="author-information-content"><span class="c-article-author-information__subtitle u-visually-hidden" id="author-notes">Author notes</span><ol class="c-article-author-information__list"><li class="c-article-author-information__item" id="nAff14"><p class="c-article-author-information__authors-list">Edin Muratspahić</p><p class="js-present-address">Present address: Institute for Protein Design, University of Washington, Seattle, WA, 98195, USA</p></li><li class="c-article-author-information__item" id="nAff15"><p class="c-article-author-information__authors-list">Kristine Deibler</p><p class="js-present-address">Present address: Novo Nordisk Research Center Seattle, Novo Nordisk A/S, 530 Fairview Ave N #5000, Seattle, WA, 97403, USA</p></li><li class="c-article-author-information__item" id="nAff16"><p class="c-article-author-information__authors-list">Johannes Koehbach</p><p class="js-present-address">Present address: School of Biomedical Sciences, Faculty for Medicine, The University of Queensland, Brisbane, QLD, 4072, Australia</p></li><li class="c-article-author-information__item" id="na1"><p>These authors contributed equally: Edin Muratspahić, Kristine Deibler, Jianming Han.</p></li></ol><h3 class="c-article__sub-heading" id="affiliations">Authors and Affiliations</h3><ol class="c-article-author-affiliation__list"><li id="Aff1"><p class="c-article-author-affiliation__address">Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University of Vienna, 1090, Vienna, Austria</p><p class="c-article-author-affiliation__authors-list">Edin Muratspahić, Nataša Tomašević, Roland Hellinger & Christian W. Gruber</p></li><li id="Aff2"><p class="c-article-author-affiliation__address">Institute for Protein Design, University of Washington, Seattle, WA, 98195, USA</p><p class="c-article-author-affiliation__authors-list">Kristine Deibler, Timothy W. Craven, Gaurav Bhardwaj & David Baker</p></li><li id="Aff3"><p class="c-article-author-affiliation__address">Center for Clinical Pharmacology, University of Health Sciences & Pharmacy at St. Louis and Washington University School of Medicine, St. Louis, MO, 63110, USA</p><p class="c-article-author-affiliation__authors-list">Jianming Han, Balazs R. Varga, Kevin Appourchaux, Susruta Majumdar & Tao Che</p></li><li id="Aff4"><p class="c-article-author-affiliation__address">Institute of Biological Chemistry, Faculty of Chemistry, University of Vienna, 1090, Vienna, Austria</p><p class="c-article-author-affiliation__authors-list">Kirtikumar B. Jadhav & Markus Muttenthaler</p></li><li id="Aff5"><p class="c-article-author-affiliation__address">Department of Pharmaceutical Chemistry, Institute of Pharmacy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria</p><p class="c-article-author-affiliation__authors-list">Aina-Leonor Olivé-Marti, Nadine Hochrainer & Mariana Spetea</p></li><li id="Aff6"><p class="c-article-author-affiliation__address">Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, QLD, 4072, Australia</p><p class="c-article-author-affiliation__authors-list">Johannes Koehbach & David J. Craik</p></li><li id="Aff7"><p class="c-article-author-affiliation__address">Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Baltimore, MD, 21201, USA</p><p class="c-article-author-affiliation__authors-list">Jonathan F. Fay</p></li><li id="Aff8"><p class="c-article-author-affiliation__address">Department of Pharmaceutical and Administrative Sciences, Saint Louis College of Pharmacy, University of Health Sciences & Pharmacy in St. Louis, St. Louis, MO, 63110, USA</p><p class="c-article-author-affiliation__authors-list">Mohammad Homaidur Rahman & Lamees Hegazy</p></li><li id="Aff9"><p class="c-article-author-affiliation__address">Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, 63110, USA</p><p class="c-article-author-affiliation__authors-list">Balazs R. Varga, Kevin Appourchaux, Susruta Majumdar & Tao Che</p></li><li id="Aff10"><p class="c-article-author-affiliation__address">Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, 4072, Australia</p><p class="c-article-author-affiliation__authors-list">Markus Muttenthaler</p></li><li id="Aff11"><p class="c-article-author-affiliation__address">Department of Bioengineering, Knight Campus, University of Oregon, Eugene, OR, 97403, USA</p><p class="c-article-author-affiliation__authors-list">Parisa Hosseinzadeh</p></li><li id="Aff12"><p class="c-article-author-affiliation__address">Department of Biochemistry, University of Washington, Seattle, WA, 98195, USA</p><p class="c-article-author-affiliation__authors-list">David Baker</p></li><li id="Aff13"><p class="c-article-author-affiliation__address">Howard Hughes Medical Institute, University of Washington, Seattle, Washington, WA, 98195, USA</p><p class="c-article-author-affiliation__authors-list">David Baker</p></li></ol><div class="u-js-hide u-hide-print" data-test="author-info"><span class="c-article__sub-heading">Authors</span><ol class="c-article-authors-search u-list-reset"><li id="auth-Edin-Muratspahi_-Aff1-Aff14"><span class="c-article-authors-search__title u-h3 js-search-name">Edin Muratspahić</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Edin%20Muratspahi%C4%87" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Edin%20Muratspahi%C4%87" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Edin%20Muratspahi%C4%87%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Kristine-Deibler-Aff2-Aff15"><span class="c-article-authors-search__title u-h3 js-search-name">Kristine Deibler</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Kristine%20Deibler" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Kristine%20Deibler" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Kristine%20Deibler%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Jianming-Han-Aff3"><span class="c-article-authors-search__title u-h3 js-search-name">Jianming Han</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Jianming%20Han" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Jianming%20Han" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Jianming%20Han%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Nata_a-Toma_evi_-Aff1"><span class="c-article-authors-search__title u-h3 js-search-name">Nataša Tomašević</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Nata%C5%A1a%20Toma%C5%A1evi%C4%87" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Nata%C5%A1a%20Toma%C5%A1evi%C4%87" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Nata%C5%A1a%20Toma%C5%A1evi%C4%87%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Kirtikumar_B_-Jadhav-Aff4"><span class="c-article-authors-search__title u-h3 js-search-name">Kirtikumar B. Jadhav</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Kirtikumar%20B.%20Jadhav" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Kirtikumar%20B.%20Jadhav" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Kirtikumar%20B.%20Jadhav%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Aina_Leonor-Oliv__Marti-Aff5"><span class="c-article-authors-search__title u-h3 js-search-name">Aina-Leonor Olivé-Marti</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Aina-Leonor%20Oliv%C3%A9-Marti" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Aina-Leonor%20Oliv%C3%A9-Marti" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Aina-Leonor%20Oliv%C3%A9-Marti%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Nadine-Hochrainer-Aff5"><span class="c-article-authors-search__title u-h3 js-search-name">Nadine Hochrainer</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Nadine%20Hochrainer" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Nadine%20Hochrainer" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Nadine%20Hochrainer%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Roland-Hellinger-Aff1"><span class="c-article-authors-search__title u-h3 js-search-name">Roland Hellinger</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Roland%20Hellinger" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Roland%20Hellinger" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Roland%20Hellinger%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Johannes-Koehbach-Aff6-Aff16"><span class="c-article-authors-search__title u-h3 js-search-name">Johannes Koehbach</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Johannes%20Koehbach" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Johannes%20Koehbach" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Johannes%20Koehbach%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Jonathan_F_-Fay-Aff7"><span class="c-article-authors-search__title u-h3 js-search-name">Jonathan F. Fay</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Jonathan%20F.%20Fay" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Jonathan%20F.%20Fay" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Jonathan%20F.%20Fay%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Mohammad_Homaidur-Rahman-Aff8"><span class="c-article-authors-search__title u-h3 js-search-name">Mohammad Homaidur Rahman</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Mohammad%20Homaidur%20Rahman" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Mohammad%20Homaidur%20Rahman" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Mohammad%20Homaidur%20Rahman%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Lamees-Hegazy-Aff8"><span class="c-article-authors-search__title u-h3 js-search-name">Lamees Hegazy</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Lamees%20Hegazy" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Lamees%20Hegazy" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Lamees%20Hegazy%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Timothy_W_-Craven-Aff2"><span class="c-article-authors-search__title u-h3 js-search-name">Timothy W. Craven</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Timothy%20W.%20Craven" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Timothy%20W.%20Craven" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Timothy%20W.%20Craven%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Balazs_R_-Varga-Aff3-Aff9"><span class="c-article-authors-search__title u-h3 js-search-name">Balazs R. Varga</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Balazs%20R.%20Varga" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Balazs%20R.%20Varga" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Balazs%20R.%20Varga%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Gaurav-Bhardwaj-Aff2"><span class="c-article-authors-search__title u-h3 js-search-name">Gaurav Bhardwaj</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Gaurav%20Bhardwaj" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Gaurav%20Bhardwaj" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Gaurav%20Bhardwaj%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Kevin-Appourchaux-Aff3-Aff9"><span class="c-article-authors-search__title u-h3 js-search-name">Kevin Appourchaux</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Kevin%20Appourchaux" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Kevin%20Appourchaux" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Kevin%20Appourchaux%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Susruta-Majumdar-Aff3-Aff9"><span class="c-article-authors-search__title u-h3 js-search-name">Susruta Majumdar</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Susruta%20Majumdar" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Susruta%20Majumdar" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Susruta%20Majumdar%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Markus-Muttenthaler-Aff4-Aff10"><span class="c-article-authors-search__title u-h3 js-search-name">Markus Muttenthaler</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Markus%20Muttenthaler" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Markus%20Muttenthaler" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Markus%20Muttenthaler%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Parisa-Hosseinzadeh-Aff11"><span class="c-article-authors-search__title u-h3 js-search-name">Parisa Hosseinzadeh</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Parisa%20Hosseinzadeh" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Parisa%20Hosseinzadeh" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Parisa%20Hosseinzadeh%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-David_J_-Craik-Aff6"><span class="c-article-authors-search__title u-h3 js-search-name">David J. Craik</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=David%20J.%20Craik" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=David%20J.%20Craik" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22David%20J.%20Craik%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Mariana-Spetea-Aff5"><span class="c-article-authors-search__title u-h3 js-search-name">Mariana Spetea</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Mariana%20Spetea" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Mariana%20Spetea" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Mariana%20Spetea%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Tao-Che-Aff3-Aff9"><span class="c-article-authors-search__title u-h3 js-search-name">Tao Che</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Tao%20Che" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Tao%20Che" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Tao%20Che%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-David-Baker-Aff2-Aff12-Aff13"><span class="c-article-authors-search__title u-h3 js-search-name">David Baker</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=David%20Baker" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=David%20Baker" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22David%20Baker%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Christian_W_-Gruber-Aff1"><span class="c-article-authors-search__title u-h3 js-search-name">Christian W. Gruber</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?author=Christian%20W.%20Gruber" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Christian%20W.%20Gruber" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Christian%20W.%20Gruber%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li></ol></div><h3 class="c-article__sub-heading" id="contributions">Contributions</h3><p>C.W.G. and G.B. initiated the project. K.D. performed computational peptide design. T.W.C. generated initial parameter files. E.M., R.H., J.K., and K.B.J. performed peptide synthesis and analysis. E.M., N.T., J.H., K.A., A.-L.O.-M., and N.H. conducted in vitro pharmacological studies. M.H.R. and L.H. performed and analyzed the MD simulations. A.-L.O.-M. and M.S. conducted in vivo pharmacological studies. J.H., J.F.F., and T.C. performed protein purification, cryo-EM studies and structural analysis. J.K., B.R.V., D.J.C., M.M., S.M., M.S., P.H., and D.B. contributed reagents and tools. All authors analyzed data. All authors contributed to drafting the manuscript. E.M., K.D., T.C., D.B., and C.W.G. edited the manuscript for submission.</p><h3 class="c-article__sub-heading" id="corresponding-author">Corresponding authors</h3><p id="corresponding-author-list">Correspondence to <a id="corresp-c1" href="mailto:taoche@wustl.edu">Tao Che</a>, <a id="corresp-c2" href="mailto:dabaker@uw.edu">David Baker</a> or <a id="corresp-c3" href="mailto:christian.w.gruber@meduniwien.ac.at">Christian W. Gruber</a>.</p></div></div></section><section data-title="Ethics declarations"><div class="c-article-section" id="ethics-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="ethics">Ethics declarations</h2><div class="c-article-section__content" id="ethics-content"> <h3 class="c-article__sub-heading" id="FPar2">Competing interests</h3> <p>S.M. is a co-founder of Sparian Biosciences. The other authors declare no competing interests.</p> </div></div></section><section data-title="Peer review"><div class="c-article-section" id="peer-review-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="peer-review">Peer review</h2><div class="c-article-section__content" id="peer-review-content"> <h3 class="c-article__sub-heading" id="FPar1">Peer review information</h3> <p><i>Nature Communications</i> thanks the anonymous reviewers for their contribution to the peer review of this work. A peer review file is available.</p> </div></div></section><section data-title="Additional information"><div class="c-article-section" id="additional-information-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="additional-information">Additional information</h2><div class="c-article-section__content" id="additional-information-content"><p><b>Publisher’s note</b> Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.</p></div></div></section><section data-title="Supplementary information"><div class="c-article-section" id="Sec31-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec31">Supplementary information</h2><div class="c-article-section__content" id="Sec31-content"><div data-test="supplementary-info"><div id="figshareContainer" class="c-article-figshare-container" data-test="figshare-container"></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM1"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="supplementary information" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM1_ESM.pdf" data-supp-info-image="">Supplementary Information</a></h3></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM2"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="peer review file" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM2_ESM.pdf" data-supp-info-image="">Peer Review File</a></h3></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM3"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="description of additional supplementary files" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM3_ESM.pdf" data-supp-info-image="">Description of Additional Supplementary Files</a></h3></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM4"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="supplementary data 1" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM4_ESM.txt" data-supp-info-image="">Supplementary Data 1</a></h3></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM5"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="supplementary data 2" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM5_ESM.txt" data-supp-info-image="">Supplementary Data 2</a></h3></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM6"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="supplementary data 3" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM6_ESM.txt" data-supp-info-image="">Supplementary Data 3</a></h3></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM7"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="supplementary data 4" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM7_ESM.txt" data-supp-info-image="">Supplementary Data 4</a></h3></div><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM8"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="reporting summary" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM8_ESM.pdf" data-supp-info-image="">Reporting Summary</a></h3></div></div></div></div></section><section data-title="Source data"><div class="c-article-section" id="Sec32-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec32">Source data</h2><div class="c-article-section__content" id="Sec32-content"><div data-test="supplementary-info"><div class="c-article-supplementary__item" data-test="supp-item" id="MOESM9"><h3 class="c-article-supplementary__title u-h3"><a class="print-link" data-track="click" data-track-action="view supplementary info" data-test="supp-info-link" data-track-label="source data" href="https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-43718-w/MediaObjects/41467_2023_43718_MOESM9_ESM.xlsx" data-supp-info-image="">Source Data</a></h3></div></div></div></div></section><section data-title="Rights and permissions"><div class="c-article-section" id="rightslink-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="rightslink">Rights and permissions</h2><div class="c-article-section__content" id="rightslink-content"> <p><b>Open Access</b> This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit <a href="http://creativecommons.org/licenses/by/4.0/" rel="license">http://creativecommons.org/licenses/by/4.0/</a>.</p> <p class="c-article-rights"><a data-track="click" data-track-action="view rights and permissions" data-track-label="link" href="https://s100.copyright.com/AppDispatchServlet?title=Design%20and%20structural%20validation%20of%20peptide%E2%80%93drug%20conjugate%20ligands%20of%20the%20kappa-opioid%20receptor&author=Edin%20Muratspahi%C4%87%20et%20al&contentID=10.1038%2Fs41467-023-43718-w&copyright=The%20Author%28s%29&publication=2041-1723&publicationDate=2023-12-06&publisherName=SpringerNature&orderBeanReset=true&oa=CC%20BY">Reprints and permissions</a></p></div></div></section><section aria-labelledby="article-info" data-title="About this article"><div class="c-article-section" id="article-info-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="article-info">About this article</h2><div class="c-article-section__content" id="article-info-content"><div class="c-bibliographic-information"><div class="u-hide-print c-bibliographic-information__column c-bibliographic-information__column--border"><a data-crossmark="10.1038/s41467-023-43718-w" target="_blank" rel="noopener" href="https://crossmark.crossref.org/dialog/?doi=10.1038/s41467-023-43718-w" data-track="click" data-track-action="Click Crossmark" data-track-label="link" data-test="crossmark"><img loading="lazy" width="57" height="81" alt="Check for updates. Verify currency and authenticity via CrossMark" src="data:image/svg+xml;base64,<svg height="81" width="57" xmlns="http://www.w3.org/2000/svg"><g fill="none" fill-rule="evenodd"><path d="m17.35 35.45 21.3-14.2v-17.03h-21.3" fill="#989898"/><path d="m38.65 35.45-21.3-14.2v-17.03h21.3" fill="#747474"/><path d="m28 .5c-12.98 0-23.5 10.52-23.5 23.5s10.52 23.5 23.5 23.5 23.5-10.52 23.5-23.5c0-6.23-2.48-12.21-6.88-16.62-4.41-4.4-10.39-6.88-16.62-6.88zm0 41.25c-9.8 0-17.75-7.95-17.75-17.75s7.95-17.75 17.75-17.75 17.75 7.95 17.75 17.75c0 4.71-1.87 9.22-5.2 12.55s-7.84 5.2-12.55 5.2z" fill="#535353"/><path d="m41 36c-5.81 6.23-15.23 7.45-22.43 2.9-7.21-4.55-10.16-13.57-7.03-21.5l-4.92-3.11c-4.95 10.7-1.19 23.42 8.78 29.71 9.97 6.3 23.07 4.22 30.6-4.86z" fill="#9c9c9c"/><path d="m.2 58.45c0-.75.11-1.42.33-2.01s.52-1.09.91-1.5c.38-.41.83-.73 1.34-.94.51-.22 1.06-.32 1.65-.32.56 0 1.06.11 1.51.35.44.23.81.5 1.1.81l-.91 1.01c-.24-.24-.49-.42-.75-.56-.27-.13-.58-.2-.93-.2-.39 0-.73.08-1.05.23-.31.16-.58.37-.81.66-.23.28-.41.63-.53 1.04-.13.41-.19.88-.19 1.39 0 1.04.23 1.86.68 2.46.45.59 1.06.88 1.84.88.41 0 .77-.07 1.07-.23s.59-.39.85-.68l.91 1c-.38.43-.8.76-1.28.99-.47.22-1 .34-1.58.34-.59 0-1.13-.1-1.64-.31-.5-.2-.94-.51-1.31-.91-.38-.4-.67-.9-.88-1.48-.22-.59-.33-1.26-.33-2.02zm8.4-5.33h1.61v2.54l-.05 1.33c.29-.27.61-.51.96-.72s.76-.31 1.24-.31c.73 0 1.27.23 1.61.71.33.47.5 1.14.5 2.02v4.31h-1.61v-4.1c0-.57-.08-.97-.25-1.21-.17-.23-.45-.35-.83-.35-.3 0-.56.08-.79.22-.23.15-.49.36-.78.64v4.8h-1.61zm7.37 6.45c0-.56.09-1.06.26-1.51.18-.45.42-.83.71-1.14.29-.3.63-.54 1.01-.71.39-.17.78-.25 1.18-.25.47 0 .88.08 1.23.24.36.16.65.38.89.67s.42.63.54 1.03c.12.41.18.84.18 1.32 0 .32-.02.57-.07.76h-4.36c.07.62.29 1.1.65 1.44.36.33.82.5 1.38.5.29 0 .57-.04.83-.13s.51-.21.76-.37l.55 1.01c-.33.21-.69.39-1.09.53-.41.14-.83.21-1.26.21-.48 0-.92-.08-1.34-.25-.41-.16-.76-.4-1.07-.7-.31-.31-.55-.69-.72-1.13-.18-.44-.26-.95-.26-1.52zm4.6-.62c0-.55-.11-.98-.34-1.28-.23-.31-.58-.47-1.06-.47-.41 0-.77.15-1.07.45-.31.29-.5.73-.58 1.3zm2.5.62c0-.57.09-1.08.28-1.53.18-.44.43-.82.75-1.13s.69-.54 1.1-.71c.42-.16.85-.24 1.31-.24.45 0 .84.08 1.17.23s.61.34.85.57l-.77 1.02c-.19-.16-.38-.28-.56-.37-.19-.09-.39-.14-.61-.14-.56 0-1.01.21-1.35.63-.35.41-.52.97-.52 1.67 0 .69.17 1.24.51 1.66.34.41.78.62 1.32.62.28 0 .54-.06.78-.17.24-.12.45-.26.64-.42l.67 1.03c-.33.29-.69.51-1.08.65-.39.15-.78.23-1.18.23-.46 0-.9-.08-1.31-.24-.4-.16-.75-.39-1.05-.7s-.53-.69-.7-1.13c-.17-.45-.25-.96-.25-1.53zm6.91-6.45h1.58v6.17h.05l2.54-3.16h1.77l-2.35 2.8 2.59 4.07h-1.75l-1.77-2.98-1.08 1.23v1.75h-1.58zm13.69 1.27c-.25-.11-.5-.17-.75-.17-.58 0-.87.39-.87 1.16v.75h1.34v1.27h-1.34v5.6h-1.61v-5.6h-.92v-1.2l.92-.07v-.72c0-.35.04-.68.13-.98.08-.31.21-.57.4-.79s.42-.39.71-.51c.28-.12.63-.18 1.04-.18.24 0 .48.02.69.07.22.05.41.1.57.17zm.48 5.18c0-.57.09-1.08.27-1.53.17-.44.41-.82.72-1.13.3-.31.65-.54 1.04-.71.39-.16.8-.24 1.23-.24s.84.08 1.24.24c.4.17.74.4 1.04.71s.54.69.72 1.13c.19.45.28.96.28 1.53s-.09 1.08-.28 1.53c-.18.44-.42.82-.72 1.13s-.64.54-1.04.7-.81.24-1.24.24-.84-.08-1.23-.24-.74-.39-1.04-.7c-.31-.31-.55-.69-.72-1.13-.18-.45-.27-.96-.27-1.53zm1.65 0c0 .69.14 1.24.43 1.66.28.41.68.62 1.18.62.51 0 .9-.21 1.19-.62.29-.42.44-.97.44-1.66 0-.7-.15-1.26-.44-1.67-.29-.42-.68-.63-1.19-.63-.5 0-.9.21-1.18.63-.29.41-.43.97-.43 1.67zm6.48-3.44h1.33l.12 1.21h.05c.24-.44.54-.79.88-1.02.35-.24.7-.36 1.07-.36.32 0 .59.05.78.14l-.28 1.4-.33-.09c-.11-.01-.23-.02-.38-.02-.27 0-.56.1-.86.31s-.55.58-.77 1.1v4.2h-1.61zm-47.87 15h1.61v4.1c0 .57.08.97.25 1.2.17.24.44.35.81.35.3 0 .57-.07.8-.22.22-.15.47-.39.73-.73v-4.7h1.61v6.87h-1.32l-.12-1.01h-.04c-.3.36-.63.64-.98.86-.35.21-.76.32-1.24.32-.73 0-1.27-.24-1.61-.71-.33-.47-.5-1.14-.5-2.02zm9.46 7.43v2.16h-1.61v-9.59h1.33l.12.72h.05c.29-.24.61-.45.97-.63.35-.17.72-.26 1.1-.26.43 0 .81.08 1.15.24.33.17.61.4.84.71.24.31.41.68.53 1.11.13.42.19.91.19 1.44 0 .59-.09 1.11-.25 1.57-.16.47-.38.85-.65 1.16-.27.32-.58.56-.94.73-.35.16-.72.25-1.1.25-.3 0-.6-.07-.9-.2s-.59-.31-.87-.56zm0-2.3c.26.22.5.37.73.45.24.09.46.13.66.13.46 0 .84-.2 1.15-.6.31-.39.46-.98.46-1.77 0-.69-.12-1.22-.35-1.61-.23-.38-.61-.57-1.13-.57-.49 0-.99.26-1.52.77zm5.87-1.69c0-.56.08-1.06.25-1.51.16-.45.37-.83.65-1.14.27-.3.58-.54.93-.71s.71-.25 1.08-.25c.39 0 .73.07 1 .2.27.14.54.32.81.55l-.06-1.1v-2.49h1.61v9.88h-1.33l-.11-.74h-.06c-.25.25-.54.46-.88.64-.33.18-.69.27-1.06.27-.87 0-1.56-.32-2.07-.95s-.76-1.51-.76-2.65zm1.67-.01c0 .74.13 1.31.4 1.7.26.38.65.58 1.15.58.51 0 .99-.26 1.44-.77v-3.21c-.24-.21-.48-.36-.7-.45-.23-.08-.46-.12-.7-.12-.45 0-.82.19-1.13.59-.31.39-.46.95-.46 1.68zm6.35 1.59c0-.73.32-1.3.97-1.71.64-.4 1.67-.68 3.08-.84 0-.17-.02-.34-.07-.51-.05-.16-.12-.3-.22-.43s-.22-.22-.38-.3c-.15-.06-.34-.1-.58-.1-.34 0-.68.07-1 .2s-.63.29-.93.47l-.59-1.08c.39-.24.81-.45 1.28-.63.47-.17.99-.26 1.54-.26.86 0 1.51.25 1.93.76s.63 1.25.63 2.21v4.07h-1.32l-.12-.76h-.05c-.3.27-.63.48-.98.66s-.73.27-1.14.27c-.61 0-1.1-.19-1.48-.56-.38-.36-.57-.85-.57-1.46zm1.57-.12c0 .3.09.53.27.67.19.14.42.21.71.21.28 0 .54-.07.77-.2s.48-.31.73-.56v-1.54c-.47.06-.86.13-1.18.23-.31.09-.57.19-.76.31s-.33.25-.41.4c-.09.15-.13.31-.13.48zm6.29-3.63h-.98v-1.2l1.06-.07.2-1.88h1.34v1.88h1.75v1.27h-1.75v3.28c0 .8.32 1.2.97 1.2.12 0 .24-.01.37-.04.12-.03.24-.07.34-.11l.28 1.19c-.19.06-.4.12-.64.17-.23.05-.49.08-.76.08-.4 0-.74-.06-1.02-.18-.27-.13-.49-.3-.67-.52-.17-.21-.3-.48-.37-.78-.08-.3-.12-.64-.12-1.01zm4.36 2.17c0-.56.09-1.06.27-1.51s.41-.83.71-1.14c.29-.3.63-.54 1.01-.71.39-.17.78-.25 1.18-.25.47 0 .88.08 1.23.24.36.16.65.38.89.67s.42.63.54 1.03c.12.41.18.84.18 1.32 0 .32-.02.57-.07.76h-4.37c.08.62.29 1.1.65 1.44.36.33.82.5 1.38.5.3 0 .58-.04.84-.13.25-.09.51-.21.76-.37l.54 1.01c-.32.21-.69.39-1.09.53s-.82.21-1.26.21c-.47 0-.92-.08-1.33-.25-.41-.16-.77-.4-1.08-.7-.3-.31-.54-.69-.72-1.13-.17-.44-.26-.95-.26-1.52zm4.61-.62c0-.55-.11-.98-.34-1.28-.23-.31-.58-.47-1.06-.47-.41 0-.77.15-1.08.45-.31.29-.5.73-.57 1.3zm3.01 2.23c.31.24.61.43.92.57.3.13.63.2.98.2.38 0 .65-.08.83-.23s.27-.35.27-.6c0-.14-.05-.26-.13-.37-.08-.1-.2-.2-.34-.28-.14-.09-.29-.16-.47-.23l-.53-.22c-.23-.09-.46-.18-.69-.3-.23-.11-.44-.24-.62-.4s-.33-.35-.45-.55c-.12-.21-.18-.46-.18-.75 0-.61.23-1.1.68-1.49.44-.38 1.06-.57 1.83-.57.48 0 .91.08 1.29.25s.71.36.99.57l-.74.98c-.24-.17-.49-.32-.73-.42-.25-.11-.51-.16-.78-.16-.35 0-.6.07-.76.21-.17.15-.25.33-.25.54 0 .14.04.26.12.36s.18.18.31.26c.14.07.29.14.46.21l.54.19c.23.09.47.18.7.29s.44.24.64.4c.19.16.34.35.46.58.11.23.17.5.17.82 0 .3-.06.58-.17.83-.12.26-.29.48-.51.68-.23.19-.51.34-.84.45-.34.11-.72.17-1.15.17-.48 0-.95-.09-1.41-.27-.46-.19-.86-.41-1.2-.68z" fill="#535353"/></g></svg>"></a></div><div class="c-bibliographic-information__column"><h3 class="c-article__sub-heading" id="citeas">Cite this article</h3><p class="c-bibliographic-information__citation">Muratspahić, E., Deibler, K., Han, J. <i>et al.</i> Design and structural validation of peptide–drug conjugate ligands of the kappa-opioid receptor. <i>Nat Commun</i> <b>14</b>, 8064 (2023). https://doi.org/10.1038/s41467-023-43718-w</p><p class="c-bibliographic-information__download-citation u-hide-print"><a data-test="citation-link" data-track="click" data-track-action="download article citation" data-track-label="link" data-track-external="" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1038/s41467-023-43718-w?format=refman&flavour=citation">Download citation<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></p><ul class="c-bibliographic-information__list" data-test="publication-history"><li class="c-bibliographic-information__list-item"><p>Received<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2023-02-13">13 February 2023</time></span></p></li><li class="c-bibliographic-information__list-item"><p>Accepted<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2023-11-17">17 November 2023</time></span></p></li><li class="c-bibliographic-information__list-item"><p>Published<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2023-12-06">06 December 2023</time></span></p></li><li class="c-bibliographic-information__list-item c-bibliographic-information__list-item--full-width"><p><abbr title="Digital Object Identifier">DOI</abbr><span class="u-hide">: </span><span class="c-bibliographic-information__value">https://doi.org/10.1038/s41467-023-43718-w</span></p></li></ul><div data-component="share-box"><div class="c-article-share-box u-display-none" hidden=""><h3 class="c-article__sub-heading">Share this article</h3><p class="c-article-share-box__description">Anyone you share the following link with will be able to read this content:</p><button class="js-get-share-url c-article-share-box__button" type="button" id="get-share-url" data-track="click" data-track-label="button" data-track-external="" data-track-action="get shareable link">Get shareable link</button><div class="js-no-share-url-container u-display-none" hidden=""><p class="js-c-article-share-box__no-sharelink-info c-article-share-box__no-sharelink-info">Sorry, a shareable link is not currently available for this article.</p></div><div class="js-share-url-container u-display-none" hidden=""><p class="js-share-url c-article-share-box__only-read-input" id="share-url" data-track="click" data-track-label="button" data-track-action="select share url"></p><button class="js-copy-share-url c-article-share-box__button--link-like" type="button" id="copy-share-url" data-track="click" data-track-label="button" data-track-action="copy share url" data-track-external="">Copy to clipboard</button></div><p class="js-c-article-share-box__additional-info c-article-share-box__additional-info"> Provided by the Springer Nature SharedIt content-sharing initiative </p></div></div><div data-component="article-info-list"></div></div></div></div></div></section> </div> <section> <div class="c-article-section js-article-section" id="further-reading-section" data-test="further-reading-section"> <h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="further-reading">This article is cited by</h2> <div class="c-article-section__content js-collapsible-section" id="further-reading-content"> <ul class="c-article-further-reading__list" id="further-reading-list"> <li class="c-article-further-reading__item js-ref-item"> <h3 class="c-article-further-reading__title" data-test="article-title"> <a class="print-link" data-track="click" data-track-action="view further reading article" data-track-label="link:Mechanism of antagonist ligand binding to REV-ERBα" href="https://doi.org/10.1038/s41598-024-58945-4"> Mechanism of antagonist ligand binding to REV-ERBα </a> </h3> <ul data-test="author-list" class="c-author-list c-author-list--compact u-sans-serif u-mb-4 u-mt-auto"> <li>Mohammad Homaidur Rahman</li><li>Lamees Hegazy</li> </ul> <p class="c-article-further-reading__journal-title"><i>Scientific Reports</i> (2024)</p> </li> </ul> </div> </div> </section> </div> </article> </main> <aside class="c-article-extras u-hide-print" aria-label="Article navigation" data-component-reading-companion data-container-type="reading-companion" data-track-component="reading companion"> <div class="js-context-bar-sticky-point-desktop" data-track-context="reading companion"> <div class="c-pdf-download u-clear-both js-pdf-download"> <a href="/articles/s41467-023-43718-w.pdf" class="u-button u-button--full-width u-button--primary u-justify-content-space-between c-pdf-download__link" data-article-pdf="true" data-readcube-pdf-url="true" data-test="download-pdf" data-draft-ignore="true" data-track="content_download" data-track-type="article pdf download" data-track-action="download pdf" data-track-label="link" data-track-external download> <span class="c-pdf-download__text">Download PDF</span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"><use xlink:href="#icon-download"/></svg> </a> </div> </div> <div class="c-reading-companion"> <div class="c-reading-companion__sticky" data-component="reading-companion-sticky" data-test="reading-companion-sticky"> <div class="c-reading-companion__panel c-reading-companion__sections c-reading-companion__panel--active" id="tabpanel-sections"> <div class="u-lazy-ad-wrapper u-mt-16 u-hide" data-component-mpu> <div class="c-ad c-ad--300x250"> <div class="c-ad__inner"> <p class="c-ad__label">Advertisement</p> <div id="div-gpt-ad-right-2" class="div-gpt-ad advert medium-rectangle js-ad text-center hide-print grade-c-hide" data-ad-type="right" data-test="right-ad" data-pa11y-ignore data-gpt data-gpt-unitpath="/285/nature_communications/article" data-gpt-sizes="300x250" data-gpt-targeting="type=article;pos=right;artid=s41467-023-43718-w;doi=10.1038/s41467-023-43718-w;techmeta=101,118,119,28,49,60,64,75,95,96;subjmeta=114,154,2387,436,469,611,631,92;kwrd=Peptides,Protein+design,Receptor+pharmacology"> <noscript> <a href="//pubads.g.doubleclick.net/gampad/jump?iu=/285/nature_communications/article&sz=300x250&c=-1797922801&t=pos%3Dright%26type%3Darticle%26artid%3Ds41467-023-43718-w%26doi%3D10.1038/s41467-023-43718-w%26techmeta%3D101,118,119,28,49,60,64,75,95,96%26subjmeta%3D114,154,2387,436,469,611,631,92%26kwrd%3DPeptides,Protein+design,Receptor+pharmacology"> <img data-test="gpt-advert-fallback-img" src="//pubads.g.doubleclick.net/gampad/ad?iu=/285/nature_communications/article&sz=300x250&c=-1797922801&t=pos%3Dright%26type%3Darticle%26artid%3Ds41467-023-43718-w%26doi%3D10.1038/s41467-023-43718-w%26techmeta%3D101,118,119,28,49,60,64,75,95,96%26subjmeta%3D114,154,2387,436,469,611,631,92%26kwrd%3DPeptides,Protein+design,Receptor+pharmacology" alt="Advertisement" width="300" height="250"></a> </noscript> </div> </div> </div> </div> </div> <div class="c-reading-companion__panel c-reading-companion__figures c-reading-companion__panel--full-width" id="tabpanel-figures"></div> <div class="c-reading-companion__panel c-reading-companion__references c-reading-companion__panel--full-width" id="tabpanel-references"></div> </div> </div> </aside> </div> <nav class="c-header__dropdown" aria-labelledby="Explore-content" data-test="Explore-content" id="explore" data-track-component="nature-150-split-header"> <div class="c-header__container"> <h2 id="Explore-content" class="c-header__heading c-header__heading--js-hide">Explore content</h2> <ul class="c-header__list c-header__list--js-stack"> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/research-articles" data-track="click" data-track-action="research articles" data-track-label="link" data-test="explore-nav-item"> Research articles </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/reviews-and-analysis" data-track="click" data-track-action="reviews & analysis" data-track-label="link" data-test="explore-nav-item"> Reviews & Analysis </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/news-and-comment" data-track="click" data-track-action="news & comment" data-track-label="link" data-test="explore-nav-item"> News & Comment </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/video" data-track="click" data-track-action="videos" data-track-label="link" data-test="explore-nav-item"> Videos </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/collections" data-track="click" data-track-action="collections" data-track-label="link" data-test="explore-nav-item"> Collections </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/browse-subjects" data-track="click" data-track-action="subjects" data-track-label="link" data-test="explore-nav-item"> Subjects </a> </li> </ul> <ul class="c-header__list c-header__list--js-stack"> <li class="c-header__item"> <a class="c-header__link" href="https://www.facebook.com/NatureCommunications" data-track="click" data-track-action="facebook" data-track-label="link">Follow us on Facebook </a> </li> <li class="c-header__item"> <a class="c-header__link" href="https://twitter.com/NatureComms" data-track="click" data-track-action="twitter" data-track-label="link">Follow us on Twitter </a> </li> <li class="c-header__item c-header__item--hide-lg"> <a class="c-header__link" href="https://www.nature.com/my-account/alerts/subscribe-journal?list-id=264" rel="nofollow" data-track="click" data-track-action="Sign up for alerts" data-track-external data-track-label="link (mobile dropdown)">Sign up for alerts<svg role="img" aria-hidden="true" focusable="false" height="18" viewBox="0 0 18 18" width="18" xmlns="http://www.w3.org/2000/svg"><path d="m4 10h2.5c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-3.08578644l-1.12132034 1.1213203c-.18753638.1875364-.29289322.4418903-.29289322.7071068v.1715729h14v-.1715729c0-.2652165-.1053568-.5195704-.2928932-.7071068l-1.7071068-1.7071067v-3.4142136c0-2.76142375-2.2385763-5-5-5-2.76142375 0-5 2.23857625-5 5zm3 4c0 1.1045695.8954305 2 2 2s2-.8954305 2-2zm-5 0c-.55228475 0-1-.4477153-1-1v-.1715729c0-.530433.21071368-1.0391408.58578644-1.4142135l1.41421356-1.4142136v-3c0-3.3137085 2.6862915-6 6-6s6 2.6862915 6 6v3l1.4142136 1.4142136c.3750727.3750727.5857864.8837805.5857864 1.4142135v.1715729c0 .5522847-.4477153 1-1 1h-4c0 1.6568542-1.3431458 3-3 3-1.65685425 0-3-1.3431458-3-3z" fill="#fff"/></svg> </a> </li> <li class="c-header__item c-header__item--hide-lg"> <a class="c-header__link" href="https://www.nature.com/ncomms.rss" data-track="click" data-track-action="rss feed" data-track-label="link"> <span>RSS feed</span> </a> </li> </ul> </div> </nav> <nav class="c-header__dropdown" aria-labelledby="About-the-journal" id="about-the-journal" data-test="about-the-journal" data-track-component="nature-150-split-header"> <div class="c-header__container"> <h2 id="About-the-journal" class="c-header__heading c-header__heading--js-hide">About the journal</h2> <ul class="c-header__list c-header__list--js-stack"> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/aims" data-track="click" data-track-action="aims & scope" data-track-label="link"> Aims & Scope </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/editors" data-track="click" data-track-action="editors" data-track-label="link"> Editors </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/journal-information" data-track="click" data-track-action="journal information" data-track-label="link"> Journal Information </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/open-access" data-track="click" data-track-action="open access fees and funding" data-track-label="link"> Open Access Fees and Funding </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/calls-for-papers" data-track="click" data-track-action="calls for papers" data-track-label="link"> Calls for Papers </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/editorial-values-statement" data-track="click" data-track-action="editorial values statement" data-track-label="link"> Editorial Values Statement </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/journal-impact" data-track="click" data-track-action="journal metrics" data-track-label="link"> Journal Metrics </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/editorshighlights" data-track="click" data-track-action="editors' highlights" data-track-label="link"> Editors' Highlights </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/contact" data-track="click" data-track-action="contact" data-track-label="link"> Contact </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/editorial-policies" data-track="click" data-track-action="editorial policies" data-track-label="link"> Editorial policies </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/top-articles" data-track="click" data-track-action="top articles" data-track-label="link"> Top Articles </a> </li> </ul> </div> </nav> <nav class="c-header__dropdown" aria-labelledby="Publish-with-us-label" id="publish-with-us" data-test="publish-with-us" data-track-component="nature-150-split-header"> <div class="c-header__container"> <h2 id="Publish-with-us-label" class="c-header__heading c-header__heading--js-hide">Publish with us</h2> <ul class="c-header__list c-header__list--js-stack"> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/submit" data-track="click" data-track-action="for authors" data-track-label="link"> For authors </a> </li> <li class="c-header__item"> <a class="c-header__link" href="/ncomms/for-reviewers" data-track="click" data-track-action="for reviewers" data-track-label="link"> For Reviewers </a> </li> <li class="c-header__item"> <a class="c-header__link" data-test="nature-author-services" data-track="nav_language_services" data-track-context="header publish with us dropdown menu" data-track-action="manuscript author services" data-track-label="link manuscript author services" href="https://authorservices.springernature.com/go/sn/?utm_source=For+Authors&utm_medium=Website_Nature&utm_campaign=Platform+Experimentation+2022&utm_id=PE2022"> Language editing services </a> </li> <li class="c-header__item c-header__item--keyline"> <a class="c-header__link" href="https://mts-ncomms.nature.com/" data-track="click_submit_manuscript" data-track-context="submit link in Nature header dropdown menu" data-track-action="submit manuscript" data-track-label="link (publish with us dropdown menu)" data-track-external>Submit manuscript<svg role="img" aria-hidden="true" focusable="false" height="18" viewBox="0 0 18 18" width="18" xmlns="http://www.w3.org/2000/svg"><path d="m15 0c1.1045695 0 2 .8954305 2 2v5.5c0 .27614237-.2238576.5-.5.5s-.5-.22385763-.5-.5v-5.5c0-.51283584-.3860402-.93550716-.8833789-.99327227l-.1166211-.00672773h-9v3c0 1.1045695-.8954305 2-2 2h-3v10c0 .5128358.38604019.9355072.88337887.9932723l.11662113.0067277h7.5c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-7.5c-1.1045695 0-2-.8954305-2-2v-10.17157288c0-.53043297.21071368-1.0391408.58578644-1.41421356l3.82842712-3.82842712c.37507276-.37507276.88378059-.58578644 1.41421356-.58578644zm-.5442863 8.18867991 3.3545404 3.35454039c.2508994.2508994.2538696.6596433.0035959.909917-.2429543.2429542-.6561449.2462671-.9065387-.0089489l-2.2609825-2.3045251.0010427 7.2231989c0 .3569916-.2898381.6371378-.6473715.6371378-.3470771 0-.6473715-.2852563-.6473715-.6371378l-.0010428-7.2231995-2.2611222 2.3046654c-.2531661.2580415-.6562868.2592444-.9065605.0089707-.24295423-.2429542-.24865597-.6576651.0036132-.9099343l3.3546673-3.35466731c.2509089-.25090888.6612706-.25227691.9135302-.00001728zm-.9557137-3.18867991c.2761424 0 .5.22385763.5.5s-.2238576.5-.5.5h-6c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5zm-8.5-3.587-3.587 3.587h2.587c.55228475 0 1-.44771525 1-1zm8.5 1.587c.2761424 0 .5.22385763.5.5s-.2238576.5-.5.5h-6c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5z" fill="#fff"/></svg> </a> </li> </ul> </div> </nav> <div id="search-menu" class="c-header__dropdown c-header__dropdown--full-width" data-track-component="nature-150-split-header"> <div class="c-header__container"> <h2 class="c-header__visually-hidden">Search</h2> <form class="c-header__search-form" action="/search" method="get" role="search" autocomplete="off" data-test="inline-search"> <label class="c-header__heading" for="keywords">Search articles by subject, keyword or author</label> <div class="c-header__search-layout c-header__search-layout--max-width"> <div> <input type="text" required="" class="c-header__input" id="keywords" name="q" value=""> </div> <div class="c-header__search-layout"> <div> <label for="results-from" class="c-header__visually-hidden">Show results from</label> <select id="results-from" name="journal" class="c-header__select"> <option value="" selected>All journals</option> <option value="ncomms">This journal</option> </select> </div> <div> <button type="submit" class="c-header__search-button">Search</button> </div> </div> </div> </form> <div class="c-header__flush"> <a class="c-header__link" href="/search/advanced" data-track="click" data-track-action="advanced search" data-track-label="link"> Advanced search </a> </div> <h3 class="c-header__heading c-header__heading--keyline">Quick links</h3> <ul class="c-header__list"> <li><a class="c-header__link" href="/subjects" data-track="click" data-track-action="explore articles by subject" data-track-label="link">Explore articles by subject</a></li> <li><a class="c-header__link" href="/naturecareers" data-track="click" data-track-action="find a job" data-track-label="link">Find a job</a></li> <li><a class="c-header__link" href="/authors/index.html" data-track="click" data-track-action="guide to authors" data-track-label="link">Guide to authors</a></li> <li><a class="c-header__link" href="/authors/editorial_policies/" data-track="click" data-track-action="editorial policies" data-track-label="link">Editorial policies</a></li> </ul> </div> </div> <footer class="composite-layer" itemscope itemtype="http://schema.org/Periodical"> <meta itemprop="publisher" content="Springer Nature"> <div class="u-mt-16 u-mb-16"> <div class="u-container"> <div class="u-display-flex u-flex-wrap u-justify-content-space-between"> <p class="c-meta u-ma-0 u-flex-shrink"> <span class="c-meta__item"> Nature Communications (<i>Nat Commun</i>) </span> <span class="c-meta__item"> <abbr title="International Standard Serial Number">ISSN</abbr> <span itemprop="onlineIssn">2041-1723</span> (online) </span> </p> </div> </div> </div> <div class="c-footer"> <div class="u-hide-print" data-track-component="footer"> <h2 class="u-visually-hidden">nature.com sitemap</h2> <div class="c-footer__container"> <div class="c-footer__grid c-footer__group--separator"> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">About Nature Portfolio</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/npg_/company_info/index.html" data-track="click" data-track-action="about us" data-track-label="link">About us</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/npg_/press_room/press_releases.html" data-track="click" data-track-action="press releases" data-track-label="link">Press releases</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://press.nature.com/" data-track="click" data-track-action="press office" data-track-label="link">Press office</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://support.nature.com/support/home" data-track="click" data-track-action="contact us" data-track-label="link">Contact us</a></li> </ul> </div> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">Discover content</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/siteindex" data-track="click" data-track-action="journals a-z" data-track-label="link">Journals A-Z</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/subjects" data-track="click" data-track-action="article by subject" data-track-label="link">Articles by subject</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.protocols.io/" data-track="click" data-track-action="protocols.io" data-track-label="link">protocols.io</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.natureindex.com/" data-track="click" data-track-action="nature index" data-track-label="link">Nature Index</a></li> </ul> </div> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">Publishing policies</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/authors/editorial_policies" data-track="click" data-track-action="Nature portfolio policies" data-track-label="link">Nature portfolio policies</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/nature-research/open-access" data-track="click" data-track-action="open access" data-track-label="link">Open access</a></li> </ul> </div> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">Author & Researcher services</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/reprints" data-track="click" data-track-action="reprints and permissions" data-track-label="link">Reprints & permissions</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.springernature.com/gp/authors/research-data" data-track="click" data-track-action="data research service" data-track-label="link">Research data</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://authorservices.springernature.com/language-editing/" data-track="click" data-track-action="language editing" data-track-label="link">Language editing</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://authorservices.springernature.com/scientific-editing/" data-track="click" data-track-action="scientific editing" data-track-label="link">Scientific editing</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://masterclasses.nature.com/" data-track="click" data-track-action="nature masterclasses" data-track-label="link">Nature Masterclasses</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://solutions.springernature.com/" data-track="click" data-track-action="research solutions" data-track-label="link">Research Solutions</a></li> </ul> </div> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">Libraries & institutions</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.springernature.com/gp/librarians/tools-services" data-track="click" data-track-action="librarian service and tools" data-track-label="link">Librarian service & tools</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.springernature.com/gp/librarians/manage-your-account/librarianportal" data-track="click" data-track-action="librarian portal" data-track-label="link">Librarian portal</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/openresearch/about-open-access/information-for-institutions" data-track="click" data-track-action="open research" data-track-label="link">Open research</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.springernature.com/gp/librarians/recommend-to-your-library" data-track="click" data-track-action="Recommend to library" data-track-label="link">Recommend to library</a></li> </ul> </div> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">Advertising & partnerships</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://partnerships.nature.com/product/digital-advertising/" data-track="click" data-track-action="advertising" data-track-label="link">Advertising</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://partnerships.nature.com/" data-track="click" data-track-action="partnerships and services" data-track-label="link">Partnerships & Services</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://partnerships.nature.com/media-kits/" data-track="click" data-track-action="media kits" data-track-label="link">Media kits</a> </li> <li class="c-footer__item"><a class="c-footer__link" href="https://partnerships.nature.com/product/branded-content-native-advertising/" data-track-action="branded content" data-track-label="link">Branded content</a></li> </ul> </div> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">Professional development</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/naturecareers/" data-track="click" data-track-action="nature careers" data-track-label="link">Nature Careers</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://conferences.nature.com" data-track="click" data-track-action="nature conferences" data-track-label="link">Nature<span class="u-visually-hidden"> </span> Conferences</a></li> </ul> </div> <div class="c-footer__group"> <h3 class="c-footer__heading u-mt-0">Regional websites</h3> <ul class="c-footer__list"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/natafrica" data-track="click" data-track-action="nature africa" data-track-label="link">Nature Africa</a></li> <li class="c-footer__item"><a class="c-footer__link" href="http://www.naturechina.com" data-track="click" data-track-action="nature china" data-track-label="link">Nature China</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/nindia" data-track="click" data-track-action="nature india" data-track-label="link">Nature India</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/natitaly" data-track="click" data-track-action="nature Italy" data-track-label="link">Nature Italy</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.natureasia.com/ja-jp" data-track="click" data-track-action="nature japan" data-track-label="link">Nature Japan</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/nmiddleeast" data-track="click" data-track-action="nature middle east" data-track-label="link">Nature Middle East</a></li> </ul> </div> </div> </div> <div class="c-footer__container"> <ul class="c-footer__links"> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/info/privacy" data-track="click" data-track-action="privacy policy" data-track-label="link">Privacy Policy</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/info/cookies" data-track="click" data-track-action="use of cookies" data-track-label="link">Use of cookies</a></li> <li class="c-footer__item"> <button class="optanon-toggle-display c-footer__link" onclick="javascript:;" data-cc-action="preferences" data-track="click" data-track-action="manage cookies" data-track-label="link">Your privacy choices/Manage cookies </button> </li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/info/legal-notice" data-track="click" data-track-action="legal notice" data-track-label="link">Legal notice</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/info/accessibility-statement" data-track="click" data-track-action="accessibility statement" data-track-label="link">Accessibility statement</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.nature.com/info/terms-and-conditions" data-track="click" data-track-action="terms and conditions" data-track-label="link">Terms & Conditions</a></li> <li class="c-footer__item"><a class="c-footer__link" href="https://www.springernature.com/ccpa" data-track="click" data-track-action="california privacy statement" data-track-label="link">Your US state privacy rights</a></li> </ul> </div> </div> <div class="c-footer__container"> <a href="https://www.springernature.com/" class="c-footer__link"> <img src="/static/images/logos/sn-logo-white-ea63208b81.svg" alt="Springer Nature" loading="lazy" width="200" height="20"/> </a> <p class="c-footer__legal" data-test="copyright">© 2024 Springer Nature Limited</p> </div> </div> <div class="u-visually-hidden" aria-hidden="true"> <?xml version="1.0" encoding="UTF-8"?><!DOCTYPE svg PUBLIC "-//W3C//DTD SVG 1.1//EN" "http://www.w3.org/Graphics/SVG/1.1/DTD/svg11.dtd"><svg xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><defs><path id="a" d="M0 .74h56.72v55.24H0z"/></defs><symbol id="icon-access" viewBox="0 0 18 18"><path d="m14 8c.5522847 0 1 .44771525 1 1v7h2.5c.2761424 0 .5.2238576.5.5v1.5h-18v-1.5c0-.2761424.22385763-.5.5-.5h2.5v-7c0-.55228475.44771525-1 1-1s1 .44771525 1 1v6.9996556h8v-6.9996556c0-.55228475.4477153-1 1-1zm-8 0 2 1v5l-2 1zm6 0v7l-2-1v-5zm-2.42653766-7.59857636 7.03554716 4.92488299c.4162533.29137735.5174853.86502537.226108 1.28127873-.1721584.24594054-.4534847.39241464-.7536934.39241464h-14.16284822c-.50810197 0-.92-.41189803-.92-.92 0-.30020869.1464741-.58153499.39241464-.75369337l7.03554714-4.92488299c.34432015-.2410241.80260453-.2410241 1.14692468 0zm-.57346234 2.03988748-3.65526982 2.55868888h7.31053962z" fill-rule="evenodd"/></symbol><symbol id="icon-account" viewBox="0 0 18 18"><path d="m10.2379028 16.9048051c1.3083556-.2032362 2.5118471-.7235183 3.5294683-1.4798399-.8731327-2.5141501-2.0638925-3.935978-3.7673711-4.3188248v-1.27684611c1.1651924-.41183641 2-1.52307546 2-2.82929429 0-1.65685425-1.3431458-3-3-3-1.65685425 0-3 1.34314575-3 3 0 1.30621883.83480763 2.41745788 2 2.82929429v1.27684611c-1.70347856.3828468-2.89423845 1.8046747-3.76737114 4.3188248 1.01762123.7563216 2.22111275 1.2766037 3.52946833 1.4798399.40563808.0629726.81921174.0951949 1.23790281.0951949s.83226473-.0322223 1.2379028-.0951949zm4.3421782-2.1721994c1.4927655-1.4532925 2.419919-3.484675 2.419919-5.7326057 0-4.418278-3.581722-8-8-8s-8 3.581722-8 8c0 2.2479307.92715352 4.2793132 2.41991895 5.7326057.75688473-2.0164459 1.83949951-3.6071894 3.48926591-4.3218837-1.14534283-.70360829-1.90918486-1.96796271-1.90918486-3.410722 0-2.209139 1.790861-4 4-4s4 1.790861 4 4c0 1.44275929-.763842 2.70711371-1.9091849 3.410722 1.6497664.7146943 2.7323812 2.3054378 3.4892659 4.3218837zm-5.580081 3.2673943c-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9 4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9z" fill-rule="evenodd"/></symbol><symbol id="icon-alert" viewBox="0 0 18 18"><path d="m4 10h2.5c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-3.08578644l-1.12132034 1.1213203c-.18753638.1875364-.29289322.4418903-.29289322.7071068v.1715729h14v-.1715729c0-.2652165-.1053568-.5195704-.2928932-.7071068l-1.7071068-1.7071067v-3.4142136c0-2.76142375-2.2385763-5-5-5-2.76142375 0-5 2.23857625-5 5zm3 4c0 1.1045695.8954305 2 2 2s2-.8954305 2-2zm-5 0c-.55228475 0-1-.4477153-1-1v-.1715729c0-.530433.21071368-1.0391408.58578644-1.4142135l1.41421356-1.4142136v-3c0-3.3137085 2.6862915-6 6-6s6 2.6862915 6 6v3l1.4142136 1.4142136c.3750727.3750727.5857864.8837805.5857864 1.4142135v.1715729c0 .5522847-.4477153 1-1 1h-4c0 1.6568542-1.3431458 3-3 3-1.65685425 0-3-1.3431458-3-3z" fill-rule="evenodd"/></symbol><symbol id="icon-arrow-broad" viewBox="0 0 16 16"><path d="m6.10307866 2.97190702v7.69043288l2.44965196-2.44676915c.38776071-.38730439 1.0088052-.39493524 1.38498697-.01919617.38609051.38563612.38643641 1.01053024-.00013864 1.39665039l-4.12239817 4.11754683c-.38616704.3857126-1.01187344.3861062-1.39846576-.0000311l-4.12258206-4.11773056c-.38618426-.38572979-.39254614-1.00476697-.01636437-1.38050605.38609047-.38563611 1.01018509-.38751562 1.4012233.00306241l2.44985644 2.4469734v-8.67638639c0-.54139983.43698413-.98042709.98493125-.98159081l7.89910522-.0043627c.5451687 0 .9871152.44142642.9871152.98595351s-.4419465.98595351-.9871152.98595351z" fill-rule="evenodd" transform="matrix(-1 0 0 -1 14 15)"/></symbol><symbol id="icon-arrow-down" viewBox="0 0 16 16"><path d="m3.28337502 11.5302405 4.03074001 4.176208c.37758093.3912076.98937525.3916069 1.367372-.0000316l4.03091977-4.1763942c.3775978-.3912252.3838182-1.0190815.0160006-1.4001736-.3775061-.39113013-.9877245-.39303641-1.3700683.003106l-2.39538585 2.4818345v-11.6147896l-.00649339-.11662112c-.055753-.49733869-.46370161-.88337888-.95867408-.88337888-.49497246 0-.90292107.38604019-.95867408.88337888l-.00649338.11662112v11.6147896l-2.39518594-2.4816273c-.37913917-.39282218-.98637524-.40056175-1.35419292-.0194697-.37750607.3911302-.37784433 1.0249269.00013556 1.4165479z" fill-rule="evenodd"/></symbol><symbol id="icon-arrow-left" viewBox="0 0 16 16"><path d="m4.46975946 3.28337502-4.17620792 4.03074001c-.39120768.37758093-.39160691.98937525.0000316 1.367372l4.1763942 4.03091977c.39122514.3775978 1.01908149.3838182 1.40017357.0160006.39113012-.3775061.3930364-.9877245-.00310603-1.3700683l-2.48183446-2.39538585h11.61478958l.1166211-.00649339c.4973387-.055753.8833789-.46370161.8833789-.95867408 0-.49497246-.3860402-.90292107-.8833789-.95867408l-.1166211-.00649338h-11.61478958l2.4816273-2.39518594c.39282216-.37913917.40056173-.98637524.01946965-1.35419292-.39113012-.37750607-1.02492687-.37784433-1.41654791.00013556z" fill-rule="evenodd"/></symbol><symbol id="icon-arrow-right" viewBox="0 0 16 16"><path d="m11.5302405 12.716625 4.176208-4.03074003c.3912076-.37758093.3916069-.98937525-.0000316-1.367372l-4.1763942-4.03091981c-.3912252-.37759778-1.0190815-.38381821-1.4001736-.01600053-.39113013.37750607-.39303641.98772445.003106 1.37006824l2.4818345 2.39538588h-11.6147896l-.11662112.00649339c-.49733869.055753-.88337888.46370161-.88337888.95867408 0 .49497246.38604019.90292107.88337888.95867408l.11662112.00649338h11.6147896l-2.4816273 2.39518592c-.39282218.3791392-.40056175.9863753-.0194697 1.3541929.3911302.3775061 1.0249269.3778444 1.4165479-.0001355z" fill-rule="evenodd"/></symbol><symbol id="icon-arrow-sub" viewBox="0 0 16 16"><path d="m7.89692134 4.97190702v7.69043288l-2.44965196-2.4467692c-.38776071-.38730434-1.0088052-.39493519-1.38498697-.0191961-.38609047.3856361-.38643643 1.0105302.00013864 1.3966504l4.12239817 4.1175468c.38616704.3857126 1.01187344.3861062 1.39846576-.0000311l4.12258202-4.1177306c.3861843-.3857298.3925462-1.0047669.0163644-1.380506-.3860905-.38563612-1.0101851-.38751563-1.4012233.0030624l-2.44985643 2.4469734v-8.67638639c0-.54139983-.43698413-.98042709-.98493125-.98159081l-7.89910525-.0043627c-.54516866 0-.98711517.44142642-.98711517.98595351s.44194651.98595351.98711517.98595351z" fill-rule="evenodd"/></symbol><symbol id="icon-arrow-up" viewBox="0 0 16 16"><path d="m12.716625 4.46975946-4.03074003-4.17620792c-.37758093-.39120768-.98937525-.39160691-1.367372.0000316l-4.03091981 4.1763942c-.37759778.39122514-.38381821 1.01908149-.01600053 1.40017357.37750607.39113012.98772445.3930364 1.37006824-.00310603l2.39538588-2.48183446v11.61478958l.00649339.1166211c.055753.4973387.46370161.8833789.95867408.8833789.49497246 0 .90292107-.3860402.95867408-.8833789l.00649338-.1166211v-11.61478958l2.39518592 2.4816273c.3791392.39282216.9863753.40056173 1.3541929.01946965.3775061-.39113012.3778444-1.02492687-.0001355-1.41654791z" fill-rule="evenodd"/></symbol><symbol id="icon-article" viewBox="0 0 18 18"><path d="m13 15v-12.9906311c0-.0073595-.0019884-.0093689.0014977-.0093689l-11.00158888.00087166v13.00506804c0 .5482678.44615281.9940603.99415146.9940603h10.27350412c-.1701701-.2941734-.2675644-.6357129-.2675644-1zm-12 .0059397v-13.00506804c0-.5562408.44704472-1.00087166.99850233-1.00087166h11.00299537c.5510129 0 .9985023.45190985.9985023 1.0093689v2.9906311h3v9.9914698c0 1.1065798-.8927712 2.0085302-1.9940603 2.0085302h-12.01187942c-1.09954652 0-1.99406028-.8927712-1.99406028-1.9940603zm13-9.0059397v9c0 .5522847.4477153 1 1 1s1-.4477153 1-1v-9zm-10-2h7v4h-7zm1 1v2h5v-2zm-1 4h7v1h-7zm0 2h7v1h-7zm0 2h7v1h-7z" fill-rule="evenodd"/></symbol><symbol id="icon-audio" viewBox="0 0 18 18"><path d="m13.0957477 13.5588459c-.195279.1937043-.5119137.193729-.7072234.0000551-.1953098-.193674-.1953346-.5077061-.0000556-.7014104 1.0251004-1.0168342 1.6108711-2.3905226 1.6108711-3.85745208 0-1.46604976-.5850634-2.83898246-1.6090736-3.85566829-.1951894-.19379323-.1950192-.50782531.0003802-.70141028.1953993-.19358497.512034-.19341614.7072234.00037709 1.2094886 1.20083761 1.901635 2.8250555 1.901635 4.55670148 0 1.73268608-.6929822 3.35779608-1.9037571 4.55880738zm2.1233994 2.1025159c-.195234.193749-.5118687.1938462-.7072235.0002171-.1953548-.1936292-.1954528-.5076613-.0002189-.7014104 1.5832215-1.5711805 2.4881302-3.6939808 2.4881302-5.96012998 0-2.26581266-.9046382-4.3883241-2.487443-5.95944795-.1952117-.19377107-.1950777-.50780316.0002993-.70141031s.5120117-.19347426.7072234.00029682c1.7683321 1.75528196 2.7800854 4.12911258 2.7800854 6.66056144 0 2.53182498-1.0120556 4.90597838-2.7808529 6.66132328zm-14.21898205-3.6854911c-.5523759 0-1.00016505-.4441085-1.00016505-.991944v-3.96777631c0-.54783558.44778915-.99194407 1.00016505-.99194407h2.0003301l5.41965617-3.8393633c.44948677-.31842296 1.07413994-.21516983 1.39520191.23062232.12116339.16823446.18629727.36981184.18629727.57655577v12.01603479c0 .5478356-.44778914.9919441-1.00016505.9919441-.20845738 0-.41170538-.0645985-.58133413-.184766l-5.41965617-3.8393633zm0-.991944h2.32084805l5.68047235 4.0241292v-12.01603479l-5.68047235 4.02412928h-2.32084805z" fill-rule="evenodd"/></symbol><symbol id="icon-block" viewBox="0 0 24 24"><path d="m0 0h24v24h-24z" fill-rule="evenodd"/></symbol><symbol id="icon-book" viewBox="0 0 18 18"><path d="m4 13v-11h1v11h11v-11h-13c-.55228475 0-1 .44771525-1 1v10.2675644c.29417337-.1701701.63571286-.2675644 1-.2675644zm12 1h-13c-.55228475 0-1 .4477153-1 1s.44771525 1 1 1h13zm0 3h-13c-1.1045695 0-2-.8954305-2-2v-12c0-1.1045695.8954305-2 2-2h13c.5522847 0 1 .44771525 1 1v14c0 .5522847-.4477153 1-1 1zm-8.5-13h6c.2761424 0 .5.22385763.5.5s-.2238576.5-.5.5h-6c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5zm1 2h4c.2761424 0 .5.22385763.5.5s-.2238576.5-.5.5h-4c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5z" fill-rule="evenodd"/></symbol><symbol id="icon-broad" viewBox="0 0 24 24"><path d="m9.18274226 7.81v7.7999954l2.48162734-2.4816273c.3928221-.3928221 1.0219731-.4005617 1.4030652-.0194696.3911301.3911301.3914806 1.0249268-.0001404 1.4165479l-4.17620796 4.1762079c-.39120769.3912077-1.02508144.3916069-1.41671995-.0000316l-4.1763942-4.1763942c-.39122514-.3912251-.39767006-1.0190815-.01657798-1.4001736.39113012-.3911301 1.02337106-.3930364 1.41951349.0031061l2.48183446 2.4818344v-8.7999954c0-.54911294.4426881-.99439484.99778758-.99557515l8.00221246-.00442485c.5522847 0 1 .44771525 1 1s-.4477153 1-1 1z" fill-rule="evenodd" transform="matrix(-1 0 0 -1 20.182742 24.805206)"/></symbol><symbol id="icon-calendar" viewBox="0 0 18 18"><path d="m12.5 0c.2761424 0 .5.21505737.5.49047852v.50952148h2c1.1072288 0 2 .89451376 2 2v12c0 1.1072288-.8945138 2-2 2h-12c-1.1072288 0-2-.8945138-2-2v-12c0-1.1072288.89451376-2 2-2h1v1h-1c-.55393837 0-1 .44579254-1 1v3h14v-3c0-.55393837-.4457925-1-1-1h-2v1.50952148c0 .27088381-.2319336.49047852-.5.49047852-.2761424 0-.5-.21505737-.5-.49047852v-3.01904296c0-.27088381.2319336-.49047852.5-.49047852zm3.5 7h-14v8c0 .5539384.44579254 1 1 1h12c.5539384 0 1-.4457925 1-1zm-11 6v1h-1v-1zm3 0v1h-1v-1zm3 0v1h-1v-1zm-6-2v1h-1v-1zm3 0v1h-1v-1zm6 0v1h-1v-1zm-3 0v1h-1v-1zm-3-2v1h-1v-1zm6 0v1h-1v-1zm-3 0v1h-1v-1zm-5.5-9c.27614237 0 .5.21505737.5.49047852v.50952148h5v1h-5v1.50952148c0 .27088381-.23193359.49047852-.5.49047852-.27614237 0-.5-.21505737-.5-.49047852v-3.01904296c0-.27088381.23193359-.49047852.5-.49047852z" fill-rule="evenodd"/></symbol><symbol id="icon-cart" viewBox="0 0 18 18"><path d="m5 14c1.1045695 0 2 .8954305 2 2s-.8954305 2-2 2-2-.8954305-2-2 .8954305-2 2-2zm10 0c1.1045695 0 2 .8954305 2 2s-.8954305 2-2 2-2-.8954305-2-2 .8954305-2 2-2zm-10 1c-.55228475 0-1 .4477153-1 1s.44771525 1 1 1 1-.4477153 1-1-.44771525-1-1-1zm10 0c-.5522847 0-1 .4477153-1 1s.4477153 1 1 1 1-.4477153 1-1-.4477153-1-1-1zm-12.82032249-15c.47691417 0 .88746157.33678127.98070211.80449199l.23823144 1.19501025 13.36277974.00045554c.5522847.00001882.9999659.44774934.9999659 1.00004222 0 .07084994-.0075361.14150708-.022474.2107727l-1.2908094 5.98534344c-.1007861.46742419-.5432548.80388386-1.0571651.80388386h-10.24805106c-.59173366 0-1.07142857.4477153-1.07142857 1 0 .5128358.41361449.9355072.94647737.9932723l.1249512.0067277h10.35933776c.2749512 0 .4979349.2228539.4979349.4978051 0 .2749417-.2227336.4978951-.4976753.4980063l-10.35959736.0041886c-1.18346732 0-2.14285714-.8954305-2.14285714-2 0-.6625717.34520317-1.24989198.87690425-1.61383592l-1.63768102-8.19004794c-.01312273-.06561364-.01950005-.131011-.0196107-.19547395l-1.71961253-.00064219c-.27614237 0-.5-.22385762-.5-.5 0-.27614237.22385763-.5.5-.5zm14.53193359 2.99950224h-13.11300004l1.20580469 6.02530174c.11024034-.0163252.22327998-.02480398.33844139-.02480398h10.27064786z"/></symbol><symbol id="icon-chevron-less" viewBox="0 0 10 10"><path d="m5.58578644 4-3.29289322-3.29289322c-.39052429-.39052429-.39052429-1.02368927 0-1.41421356s1.02368927-.39052429 1.41421356 0l4 4c.39052429.39052429.39052429 1.02368927 0 1.41421356l-4 4c-.39052429.39052429-1.02368927.39052429-1.41421356 0s-.39052429-1.02368927 0-1.41421356z" fill-rule="evenodd" transform="matrix(0 -1 -1 0 9 9)"/></symbol><symbol id="icon-chevron-more" viewBox="0 0 10 10"><path d="m5.58578644 6-3.29289322-3.29289322c-.39052429-.39052429-.39052429-1.02368927 0-1.41421356s1.02368927-.39052429 1.41421356 0l4 4c.39052429.39052429.39052429 1.02368927 0 1.41421356l-4 4.00000002c-.39052429.3905243-1.02368927.3905243-1.41421356 0s-.39052429-1.02368929 0-1.41421358z" fill-rule="evenodd" transform="matrix(0 1 -1 0 11 1)"/></symbol><symbol id="icon-chevron-right" viewBox="0 0 10 10"><path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/></symbol><symbol id="icon-circle-fill" viewBox="0 0 16 16"><path d="m8 14c-3.3137085 0-6-2.6862915-6-6s2.6862915-6 6-6 6 2.6862915 6 6-2.6862915 6-6 6z" fill-rule="evenodd"/></symbol><symbol id="icon-circle" viewBox="0 0 16 16"><path d="m8 12c2.209139 0 4-1.790861 4-4s-1.790861-4-4-4-4 1.790861-4 4 1.790861 4 4 4zm0 2c-3.3137085 0-6-2.6862915-6-6s2.6862915-6 6-6 6 2.6862915 6 6-2.6862915 6-6 6z" fill-rule="evenodd"/></symbol><symbol id="icon-citation" viewBox="0 0 18 18"><path d="m8.63593473 5.99995183c2.20913897 0 3.99999997 1.79084375 3.99999997 3.99996146 0 1.40730761-.7267788 2.64486871-1.8254829 3.35783281 1.6240224.6764218 2.8754442 2.0093871 3.4610603 3.6412466l-1.0763845.000006c-.5310008-1.2078237-1.5108121-2.1940153-2.7691712-2.7181346l-.79002167-.329052v-1.023992l.63016577-.4089232c.8482885-.5504661 1.3698342-1.4895187 1.3698342-2.51898361 0-1.65683828-1.3431457-2.99996146-2.99999997-2.99996146-1.65685425 0-3 1.34312318-3 2.99996146 0 1.02946491.52154569 1.96851751 1.36983419 2.51898361l.63016581.4089232v1.023992l-.79002171.329052c-1.25835905.5241193-2.23817037 1.5103109-2.76917113 2.7181346l-1.07638453-.000006c.58561612-1.6318595 1.8370379-2.9648248 3.46106024-3.6412466-1.09870405-.7129641-1.82548287-1.9505252-1.82548287-3.35783281 0-2.20911771 1.790861-3.99996146 4-3.99996146zm7.36897597-4.99995183c1.1018574 0 1.9950893.89353404 1.9950893 2.00274083v5.994422c0 1.10608317-.8926228 2.00274087-1.9950893 2.00274087l-3.0049107-.0009037v-1l3.0049107.00091329c.5490631 0 .9950893-.44783123.9950893-1.00275046v-5.994422c0-.55646537-.4450595-1.00275046-.9950893-1.00275046h-14.00982141c-.54906309 0-.99508929.44783123-.99508929 1.00275046v5.9971821c0 .66666024.33333333.99999036 1 .99999036l2-.00091329v1l-2 .0009037c-1 0-2-.99999041-2-1.99998077v-5.9971821c0-1.10608322.8926228-2.00274083 1.99508929-2.00274083zm-8.5049107 2.9999711c.27614237 0 .5.22385547.5.5 0 .2761349-.22385763.5-.5.5h-4c-.27614237 0-.5-.2238651-.5-.5 0-.27614453.22385763-.5.5-.5zm3 0c.2761424 0 .5.22385547.5.5 0 .2761349-.2238576.5-.5.5h-1c-.27614237 0-.5-.2238651-.5-.5 0-.27614453.22385763-.5.5-.5zm4 0c.2761424 0 .5.22385547.5.5 0 .2761349-.2238576.5-.5.5h-2c-.2761424 0-.5-.2238651-.5-.5 0-.27614453.2238576-.5.5-.5z" fill-rule="evenodd"/></symbol><symbol id="icon-close" viewBox="0 0 16 16"><path d="m2.29679575 12.2772478c-.39658757.3965876-.39438847 1.0328109-.00062148 1.4265779.39651227.3965123 1.03246768.3934888 1.42657791-.0006214l4.27724782-4.27724787 4.2772478 4.27724787c.3965876.3965875 1.0328109.3943884 1.4265779.0006214.3965123-.3965122.3934888-1.0324677-.0006214-1.4265779l-4.27724787-4.2772478 4.27724787-4.27724782c.3965875-.39658757.3943884-1.03281091.0006214-1.42657791-.3965122-.39651226-1.0324677-.39348875-1.4265779.00062148l-4.2772478 4.27724782-4.27724782-4.27724782c-.39658757-.39658757-1.03281091-.39438847-1.42657791-.00062148-.39651226.39651227-.39348875 1.03246768.00062148 1.42657791l4.27724782 4.27724782z" fill-rule="evenodd"/></symbol><symbol id="icon-collections" viewBox="0 0 18 18"><path d="m15 4c1.1045695 0 2 .8954305 2 2v9c0 1.1045695-.8954305 2-2 2h-8c-1.1045695 0-2-.8954305-2-2h1c0 .5128358.38604019.9355072.88337887.9932723l.11662113.0067277h8c.5128358 0 .9355072-.3860402.9932723-.8833789l.0067277-.1166211v-9c0-.51283584-.3860402-.93550716-.8833789-.99327227l-.1166211-.00672773h-1v-1zm-4-3c1.1045695 0 2 .8954305 2 2v9c0 1.1045695-.8954305 2-2 2h-8c-1.1045695 0-2-.8954305-2-2v-9c0-1.1045695.8954305-2 2-2zm0 1h-8c-.51283584 0-.93550716.38604019-.99327227.88337887l-.00672773.11662113v9c0 .5128358.38604019.9355072.88337887.9932723l.11662113.0067277h8c.5128358 0 .9355072-.3860402.9932723-.8833789l.0067277-.1166211v-9c0-.51283584-.3860402-.93550716-.8833789-.99327227zm-1.5 7c.27614237 0 .5.22385763.5.5s-.22385763.5-.5.5h-5c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5zm0-2c.27614237 0 .5.22385763.5.5s-.22385763.5-.5.5h-5c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5zm0-2c.27614237 0 .5.22385763.5.5s-.22385763.5-.5.5h-5c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5z" fill-rule="evenodd"/></symbol><symbol id="icon-compare" viewBox="0 0 18 18"><path d="m12 3c3.3137085 0 6 2.6862915 6 6s-2.6862915 6-6 6c-1.0928452 0-2.11744941-.2921742-2.99996061-.8026704-.88181407.5102749-1.90678042.8026704-3.00003939.8026704-3.3137085 0-6-2.6862915-6-6s2.6862915-6 6-6c1.09325897 0 2.11822532.29239547 3.00096303.80325037.88158756-.51107621 1.90619177-.80325037 2.99903697-.80325037zm-6 1c-2.76142375 0-5 2.23857625-5 5 0 2.7614237 2.23857625 5 5 5 .74397391 0 1.44999672-.162488 2.08451611-.4539116-1.27652344-1.1000812-2.08451611-2.7287264-2.08451611-4.5460884s.80799267-3.44600721 2.08434391-4.5463015c-.63434719-.29121054-1.34037-.4536985-2.08434391-.4536985zm6 0c-.7439739 0-1.4499967.16248796-2.08451611.45391156 1.27652341 1.10008123 2.08451611 2.72872644 2.08451611 4.54608844s-.8079927 3.4460072-2.08434391 4.5463015c.63434721.2912105 1.34037001.4536985 2.08434391.4536985 2.7614237 0 5-2.2385763 5-5 0-2.76142375-2.2385763-5-5-5zm-1.4162763 7.0005324h-3.16744736c.15614659.3572676.35283837.6927622.58425872 1.0006671h1.99892988c.23142036-.3079049.42811216-.6433995.58425876-1.0006671zm.4162763-2.0005324h-4c0 .34288501.0345146.67770871.10025909 1.0011864h3.79948181c.0657445-.32347769.1002591-.65830139.1002591-1.0011864zm-.4158423-1.99953894h-3.16831543c-.13859957.31730812-.24521946.651783-.31578599.99935097h3.79988742c-.0705665-.34756797-.1771864-.68204285-.315786-.99935097zm-1.58295822-1.999926-.08316107.06199199c-.34550042.27081213-.65446126.58611297-.91825862.93727862h2.00044041c-.28418626-.37830727-.6207872-.71499149-.99902072-.99927061z" fill-rule="evenodd"/></symbol><symbol id="icon-download-file" viewBox="0 0 18 18"><path d="m10.0046024 0c.5497429 0 1.3179837.32258606 1.707238.71184039l4.5763192 4.57631922c.3931386.39313859.7118404 1.16760135.7118404 1.71431368v8.98899651c0 1.1092806-.8945138 2.0085302-1.9940603 2.0085302h-12.01187942c-1.10128908 0-1.99406028-.8926228-1.99406028-1.9950893v-14.00982141c0-1.10185739.88743329-1.99508929 1.99961498-1.99508929zm0 1h-7.00498742c-.55709576 0-.99961498.44271433-.99961498.99508929v14.00982141c0 .5500396.44491393.9950893.99406028.9950893h12.01187942c.5463747 0 .9940603-.4506622.9940603-1.0085302v-8.98899651c0-.28393444-.2150684-.80332809-.4189472-1.0072069l-4.5763192-4.57631922c-.2038461-.20384606-.718603-.41894717-1.0001312-.41894717zm-1.5046024 4c.27614237 0 .5.21637201.5.49209595v6.14827645l1.7462789-1.77990922c.1933927-.1971171.5125222-.19455839.7001689-.0069117.1932998.19329992.1910058.50899492-.0027774.70277812l-2.59089271 2.5908927c-.19483374.1948337-.51177825.1937771-.70556873-.0000133l-2.59099079-2.5909908c-.19484111-.1948411-.19043735-.5151448-.00279066-.70279146.19329987-.19329987.50465175-.19237083.70018565.00692852l1.74638684 1.78001764v-6.14827695c0-.27177709.23193359-.49209595.5-.49209595z" fill-rule="evenodd"/></symbol><symbol id="icon-download" viewBox="0 0 16 16"><path d="m12.9975267 12.999368c.5467123 0 1.0024733.4478567 1.0024733 1.000316 0 .5563109-.4488226 1.000316-1.0024733 1.000316h-9.99505341c-.54671233 0-1.00247329-.4478567-1.00247329-1.000316 0-.5563109.44882258-1.000316 1.00247329-1.000316zm-4.9975267-11.999368c.55228475 0 1 .44497754 1 .99589209v6.80214418l2.4816273-2.48241149c.3928222-.39294628 1.0219732-.4006883 1.4030652-.01947579.3911302.39125371.3914806 1.02525073-.0001404 1.41699553l-4.17620792 4.17752758c-.39120769.3913313-1.02508144.3917306-1.41671995-.0000316l-4.17639421-4.17771394c-.39122513-.39134876-.39767006-1.01940351-.01657797-1.40061601.39113012-.39125372 1.02337105-.3931606 1.41951349.00310701l2.48183446 2.48261871v-6.80214418c0-.55001601.44386482-.99589209 1-.99589209z" fill-rule="evenodd"/></symbol><symbol id="icon-editors" viewBox="0 0 18 18"><path d="m8.72592184 2.54588137c-.48811714-.34391207-1.08343326-.54588137-1.72592184-.54588137-1.65685425 0-3 1.34314575-3 3 0 1.02947485.5215457 1.96853646 1.3698342 2.51900785l.6301658.40892721v1.02400182l-.79002171.32905522c-1.93395773.8055207-3.20997829 2.7024791-3.20997829 4.8180274v.9009805h-1v-.9009805c0-2.5479714 1.54557359-4.79153984 3.82548288-5.7411543-1.09870406-.71297106-1.82548288-1.95054399-1.82548288-3.3578652 0-2.209139 1.790861-4 4-4 1.09079823 0 2.07961816.43662103 2.80122451 1.1446278-.37707584.09278571-.7373238.22835063-1.07530267.40125357zm-2.72592184 14.45411863h-1v-.9009805c0-2.5479714 1.54557359-4.7915398 3.82548288-5.7411543-1.09870406-.71297106-1.82548288-1.95054399-1.82548288-3.3578652 0-2.209139 1.790861-4 4-4s4 1.790861 4 4c0 1.40732121-.7267788 2.64489414-1.8254829 3.3578652 2.2799093.9496145 3.8254829 3.1931829 3.8254829 5.7411543v.9009805h-1v-.9009805c0-2.1155483-1.2760206-4.0125067-3.2099783-4.8180274l-.7900217-.3290552v-1.02400184l.6301658-.40892721c.8482885-.55047139 1.3698342-1.489533 1.3698342-2.51900785 0-1.65685425-1.3431458-3-3-3-1.65685425 0-3 1.34314575-3 3 0 1.02947485.5215457 1.96853646 1.3698342 2.51900785l.6301658.40892721v1.02400184l-.79002171.3290552c-1.93395773.8055207-3.20997829 2.7024791-3.20997829 4.8180274z" fill-rule="evenodd"/></symbol><symbol id="icon-email" viewBox="0 0 18 18"><path d="m16.0049107 2c1.1018574 0 1.9950893.89706013 1.9950893 2.00585866v9.98828264c0 1.1078052-.8926228 2.0058587-1.9950893 2.0058587h-14.00982141c-1.10185739 0-1.99508929-.8970601-1.99508929-2.0058587v-9.98828264c0-1.10780515.8926228-2.00585866 1.99508929-2.00585866zm0 1h-14.00982141c-.54871518 0-.99508929.44887827-.99508929 1.00585866v9.98828264c0 .5572961.44630695 1.0058587.99508929 1.0058587h14.00982141c.5487152 0 .9950893-.4488783.9950893-1.0058587v-9.98828264c0-.55729607-.446307-1.00585866-.9950893-1.00585866zm-.0049107 2.55749512v1.44250488l-7 4-7-4v-1.44250488l7 4z" fill-rule="evenodd"/></symbol><symbol id="icon-error" viewBox="0 0 18 18"><path d="m9 0c4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9zm2.8630343 4.71100931-2.8630343 2.86303426-2.86303426-2.86303426c-.39658757-.39658757-1.03281091-.39438847-1.4265779-.00062147-.39651227.39651226-.39348876 1.03246767.00062147 1.4265779l2.86303426 2.86303426-2.86303426 2.8630343c-.39658757.3965875-.39438847 1.0328109-.00062147 1.4265779.39651226.3965122 1.03246767.3934887 1.4265779-.0006215l2.86303426-2.8630343 2.8630343 2.8630343c.3965875.3965876 1.0328109.3943885 1.4265779.0006215.3965122-.3965123.3934887-1.0324677-.0006215-1.4265779l-2.8630343-2.8630343 2.8630343-2.86303426c.3965876-.39658757.3943885-1.03281091.0006215-1.4265779-.3965123-.39651227-1.0324677-.39348876-1.4265779.00062147z" fill-rule="evenodd"/></symbol><symbol id="icon-ethics" viewBox="0 0 18 18"><path d="m6.76384967 1.41421356.83301651-.8330165c.77492941-.77492941 2.03133823-.77492941 2.80626762 0l.8330165.8330165c.3750728.37507276.8837806.58578644 1.4142136.58578644h1.3496361c1.1045695 0 2 .8954305 2 2v1.34963611c0 .53043298.2107137 1.03914081.5857864 1.41421356l.8330165.83301651c.7749295.77492941.7749295 2.03133823 0 2.80626762l-.8330165.8330165c-.3750727.3750728-.5857864.8837806-.5857864 1.4142136v1.3496361c0 1.1045695-.8954305 2-2 2h-1.3496361c-.530433 0-1.0391408.2107137-1.4142136.5857864l-.8330165.8330165c-.77492939.7749295-2.03133821.7749295-2.80626762 0l-.83301651-.8330165c-.37507275-.3750727-.88378058-.5857864-1.41421356-.5857864h-1.34963611c-1.1045695 0-2-.8954305-2-2v-1.3496361c0-.530433-.21071368-1.0391408-.58578644-1.4142136l-.8330165-.8330165c-.77492941-.77492939-.77492941-2.03133821 0-2.80626762l.8330165-.83301651c.37507276-.37507275.58578644-.88378058.58578644-1.41421356v-1.34963611c0-1.1045695.8954305-2 2-2h1.34963611c.53043298 0 1.03914081-.21071368 1.41421356-.58578644zm-1.41421356 1.58578644h-1.34963611c-.55228475 0-1 .44771525-1 1v1.34963611c0 .79564947-.31607052 1.55871121-.87867966 2.12132034l-.8330165.83301651c-.38440512.38440512-.38440512 1.00764896 0 1.39205408l.8330165.83301646c.56260914.5626092.87867966 1.3256709.87867966 2.1213204v1.3496361c0 .5522847.44771525 1 1 1h1.34963611c.79564947 0 1.55871121.3160705 2.12132034.8786797l.83301651.8330165c.38440512.3844051 1.00764896.3844051 1.39205408 0l.83301646-.8330165c.5626092-.5626092 1.3256709-.8786797 2.1213204-.8786797h1.3496361c.5522847 0 1-.4477153 1-1v-1.3496361c0-.7956495.3160705-1.5587112.8786797-2.1213204l.8330165-.83301646c.3844051-.38440512.3844051-1.00764896 0-1.39205408l-.8330165-.83301651c-.5626092-.56260913-.8786797-1.32567087-.8786797-2.12132034v-1.34963611c0-.55228475-.4477153-1-1-1h-1.3496361c-.7956495 0-1.5587112-.31607052-2.1213204-.87867966l-.83301646-.8330165c-.38440512-.38440512-1.00764896-.38440512-1.39205408 0l-.83301651.8330165c-.56260913.56260914-1.32567087.87867966-2.12132034.87867966zm3.58698944 11.4960218c-.02081224.002155-.04199226.0030286-.06345763.002542-.98766446-.0223875-1.93408568-.3063547-2.75885125-.8155622-.23496767-.1450683-.30784554-.4531483-.16277726-.688116.14506827-.2349677.45314827-.3078455.68811595-.1627773.67447084.4164161 1.44758575.6483839 2.25617384.6667123.01759529.0003988.03495764.0017019.05204365.0038639.01713363-.0017748.03452416-.0026845.05212715-.0026845 2.4852814 0 4.5-2.0147186 4.5-4.5 0-1.04888973-.3593547-2.04134635-1.0074477-2.83787157-.1742817-.21419731-.1419238-.5291218.0722736-.70340353.2141973-.17428173.5291218-.14192375.7034035.07227357.7919032.97327203 1.2317706 2.18808682 1.2317706 3.46900153 0 3.0375661-2.4624339 5.5-5.5 5.5-.02146768 0-.04261937-.0013529-.06337445-.0039782zm1.57975095-10.78419583c.2654788.07599731.419084.35281842.3430867.61829728-.0759973.26547885-.3528185.419084-.6182973.3430867-.37560116-.10752146-.76586237-.16587951-1.15568824-.17249193-2.5587807-.00064534-4.58547766 2.00216524-4.58547766 4.49928198 0 .62691557.12797645 1.23496.37274865 1.7964426.11035133.2531347-.0053975.5477984-.25853224.6581497-.25313473.1103514-.54779841-.0053975-.65814974-.2585322-.29947131-.6869568-.45606667-1.43097603-.45606667-2.1960601 0-3.05211432 2.47714695-5.50006595 5.59399617-5.49921198.48576182.00815502.96289603.0795037 1.42238033.21103795zm-1.9766658 6.41091303 2.69835-2.94655317c.1788432-.21040373.4943901-.23598862.7047939-.05714545.2104037.17884318.2359886.49439014.0571454.70479387l-3.01637681 3.34277395c-.18039088.1999106-.48669547.2210637-.69285412.0478478l-1.93095347-1.62240047c-.21213845-.17678204-.24080048-.49206439-.06401844-.70420284.17678204-.21213844.49206439-.24080048.70420284-.06401844z" fill-rule="evenodd"/></symbol><symbol id="icon-expand"><path d="M7.498 11.918a.997.997 0 0 0-.003-1.411.995.995 0 0 0-1.412-.003l-4.102 4.102v-3.51A1 1 0 0 0 .98 10.09.992.992 0 0 0 0 11.092V17c0 .554.448 1.002 1.002 1.002h5.907c.554 0 1.002-.45 1.002-1.003 0-.539-.45-.978-1.006-.978h-3.51zm3.005-5.835a.997.997 0 0 0 .003 1.412.995.995 0 0 0 1.411.003l4.103-4.103v3.51a1 1 0 0 0 1.001 1.006A.992.992 0 0 0 18 6.91V1.002A1 1 0 0 0 17 0h-5.907a1.003 1.003 0 0 0-1.002 1.003c0 .539.45.978 1.006.978h3.51z" fill-rule="evenodd"/></symbol><symbol id="icon-explore" viewBox="0 0 18 18"><path d="m9 17c4.418278 0 8-3.581722 8-8s-3.581722-8-8-8-8 3.581722-8 8 3.581722 8 8 8zm0 1c-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9 4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9zm0-2.5c-.27614237 0-.5-.2238576-.5-.5s.22385763-.5.5-.5c2.969509 0 5.400504-2.3575119 5.497023-5.31714844.0090007-.27599565.2400359-.49243782.5160315-.48343711.2759957.0090007.4924378.2400359.4834371.51603155-.114093 3.4985237-2.9869632 6.284554-6.4964916 6.284554zm-.29090657-12.99359748c.27587424-.01216621.50937715.20161139.52154336.47748563.01216621.27587423-.20161139.50937715-.47748563.52154336-2.93195733.12930094-5.25315116 2.54886451-5.25315116 5.49456849 0 .27614237-.22385763.5-.5.5s-.5-.22385763-.5-.5c0-3.48142406 2.74307146-6.34074398 6.20909343-6.49359748zm1.13784138 8.04763908-1.2004882-1.20048821c-.19526215-.19526215-.19526215-.51184463 0-.70710678s.51184463-.19526215.70710678 0l1.20048821 1.2004882 1.6006509-4.00162734-4.50670359 1.80268144-1.80268144 4.50670359zm4.10281269-6.50378907-2.6692597 6.67314927c-.1016411.2541026-.3029834.4554449-.557086.557086l-6.67314927 2.6692597 2.66925969-6.67314926c.10164107-.25410266.30298336-.45544495.55708602-.55708602z" fill-rule="evenodd"/></symbol><symbol id="icon-filter" viewBox="0 0 16 16"><path d="m14.9738641 0c.5667192 0 1.0261359.4477136 1.0261359 1 0 .24221858-.0902161.47620768-.2538899.65849851l-5.6938314 6.34147206v5.49997973c0 .3147562-.1520673.6111434-.4104543.7999971l-2.05227171 1.4999945c-.45337535.3313696-1.09655869.2418269-1.4365902-.1999993-.13321514-.1730955-.20522717-.3836284-.20522717-.5999978v-6.99997423l-5.69383133-6.34147206c-.3731872-.41563511-.32996891-1.0473954.09653074-1.41107611.18705584-.15950448.42716133-.2474224.67571519-.2474224zm-5.9218641 8.5h-2.105v6.491l.01238459.0070843.02053271.0015705.01955278-.0070558 2.0532976-1.4990996zm-8.02585008-7.5-.01564945.00240169 5.83249953 6.49759831h2.313l5.836-6.499z"/></symbol><symbol id="icon-home" viewBox="0 0 18 18"><path d="m9 5-6 6v5h4v-4h4v4h4v-5zm7 6.5857864v4.4142136c0 .5522847-.4477153 1-1 1h-5v-4h-2v4h-5c-.55228475 0-1-.4477153-1-1v-4.4142136c-.25592232 0-.51184464-.097631-.70710678-.2928932l-.58578644-.5857864c-.39052429-.3905243-.39052429-1.02368929 0-1.41421358l8.29289322-8.29289322 8.2928932 8.29289322c.3905243.39052429.3905243 1.02368928 0 1.41421358l-.5857864.5857864c-.1952622.1952622-.4511845.2928932-.7071068.2928932zm-7-9.17157284-7.58578644 7.58578644.58578644.5857864 7-6.99999996 7 6.99999996.5857864-.5857864z" fill-rule="evenodd"/></symbol><symbol id="icon-image" viewBox="0 0 18 18"><path d="m10.0046024 0c.5497429 0 1.3179837.32258606 1.707238.71184039l4.5763192 4.57631922c.3931386.39313859.7118404 1.16760135.7118404 1.71431368v8.98899651c0 1.1092806-.8945138 2.0085302-1.9940603 2.0085302h-12.01187942c-1.10128908 0-1.99406028-.8926228-1.99406028-1.9950893v-14.00982141c0-1.10185739.88743329-1.99508929 1.99961498-1.99508929zm-3.49645283 10.1752453-3.89407257 6.7495552c.11705545.048464.24538859.0751995.37998328.0751995h10.60290092l-2.4329715-4.2154691-1.57494129 2.7288098zm8.49779013 6.8247547c.5463747 0 .9940603-.4506622.9940603-1.0085302v-8.98899651c0-.28393444-.2150684-.80332809-.4189472-1.0072069l-4.5763192-4.57631922c-.2038461-.20384606-.718603-.41894717-1.0001312-.41894717h-7.00498742c-.55709576 0-.99961498.44271433-.99961498.99508929v13.98991071l4.50814957-7.81026689 3.08089884 5.33809539 1.57494129-2.7288097 3.5875735 6.2159812zm-3.0059397-11c1.1045695 0 2 .8954305 2 2s-.8954305 2-2 2-2-.8954305-2-2 .8954305-2 2-2zm0 1c-.5522847 0-1 .44771525-1 1s.4477153 1 1 1 1-.44771525 1-1-.4477153-1-1-1z" fill-rule="evenodd"/></symbol><symbol id="icon-info" viewBox="0 0 18 18"><path d="m9 0c4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9zm0 7h-1.5l-.11662113.00672773c-.49733868.05776511-.88337887.48043643-.88337887.99327227 0 .47338693.32893365.86994729.77070917.97358929l.1126697.01968298.11662113.00672773h.5v3h-.5l-.11662113.0067277c-.42082504.0488782-.76196299.3590206-.85696816.7639815l-.01968298.1126697-.00672773.1166211.00672773.1166211c.04887817.4208251.35902055.761963.76398144.8569682l.1126697.019683.11662113.0067277h3l.1166211-.0067277c.4973387-.0577651.8833789-.4804365.8833789-.9932723 0-.4733869-.3289337-.8699473-.7707092-.9735893l-.1126697-.019683-.1166211-.0067277h-.5v-4l-.00672773-.11662113c-.04887817-.42082504-.35902055-.76196299-.76398144-.85696816l-.1126697-.01968298zm0-3.25c-.69035594 0-1.25.55964406-1.25 1.25s.55964406 1.25 1.25 1.25 1.25-.55964406 1.25-1.25-.55964406-1.25-1.25-1.25z" fill-rule="evenodd"/></symbol><symbol id="icon-institution" viewBox="0 0 18 18"><path d="m7 16.9998189v-2.0003623h4v2.0003623h2v-3.0005434h-8v3.0005434zm-3-10.00181122h-1.52632364c-.27614237 0-.5-.22389817-.5-.50009056 0-.13995446.05863589-.27350497.16166338-.36820841l1.23156713-1.13206327h-2.36690687v12.00217346h3v-2.0003623h-3v-1.0001811h3v-1.0001811h1v-4.00072448h-1zm10 0v2.00036224h-1v4.00072448h1v1.0001811h3v1.0001811h-3v2.0003623h3v-12.00217346h-2.3695309l1.2315671 1.13206327c.2033191.186892.2166633.50325042.0298051.70660631-.0946863.10304615-.2282126.16169266-.3681417.16169266zm3-3.00054336c.5522847 0 1 .44779634 1 1.00018112v13.00235456h-18v-13.00235456c0-.55238478.44771525-1.00018112 1-1.00018112h3.45499992l4.20535144-3.86558216c.19129876-.17584288.48537447-.17584288.67667324 0l4.2053514 3.86558216zm-4 3.00054336h-8v1.00018112h8zm-2 6.00108672h1v-4.00072448h-1zm-1 0v-4.00072448h-2v4.00072448zm-3 0v-4.00072448h-1v4.00072448zm8-4.00072448c.5522847 0 1 .44779634 1 1.00018112v2.00036226h-2v-2.00036226c0-.55238478.4477153-1.00018112 1-1.00018112zm-12 0c.55228475 0 1 .44779634 1 1.00018112v2.00036226h-2v-2.00036226c0-.55238478.44771525-1.00018112 1-1.00018112zm5.99868798-7.81907007-5.24205601 4.81852671h10.48411203zm.00131202 3.81834559c-.55228475 0-1-.44779634-1-1.00018112s.44771525-1.00018112 1-1.00018112 1 .44779634 1 1.00018112-.44771525 1.00018112-1 1.00018112zm-1 11.00199236v1.0001811h2v-1.0001811z" fill-rule="evenodd"/></symbol><symbol id="icon-location" viewBox="0 0 18 18"><path d="m9.39521328 16.2688008c.79596342-.7770119 1.59208152-1.6299956 2.33285652-2.5295081 1.4020032-1.7024324 2.4323601-3.3624519 2.9354918-4.871847.2228715-.66861448.3364384-1.29323246.3364384-1.8674457 0-3.3137085-2.6862915-6-6-6-3.36356866 0-6 2.60156856-6 6 0 .57421324.11356691 1.19883122.3364384 1.8674457.50313169 1.5093951 1.53348863 3.1694146 2.93549184 4.871847.74077492.8995125 1.53689309 1.7524962 2.33285648 2.5295081.13694479.1336842.26895677.2602648.39521328.3793207.12625651-.1190559.25826849-.2456365.39521328-.3793207zm-.39521328 1.7311992s-7-6-7-11c0-4 3.13400675-7 7-7 3.8659932 0 7 3.13400675 7 7 0 5-7 11-7 11zm0-8c-1.65685425 0-3-1.34314575-3-3s1.34314575-3 3-3c1.6568542 0 3 1.34314575 3 3s-1.3431458 3-3 3zm0-1c1.1045695 0 2-.8954305 2-2s-.8954305-2-2-2-2 .8954305-2 2 .8954305 2 2 2z" fill-rule="evenodd"/></symbol><symbol id="icon-minus" viewBox="0 0 16 16"><path d="m2.00087166 7h11.99825664c.5527662 0 1.0008717.44386482 1.0008717 1 0 .55228475-.4446309 1-1.0008717 1h-11.99825664c-.55276616 0-1.00087166-.44386482-1.00087166-1 0-.55228475.44463086-1 1.00087166-1z" fill-rule="evenodd"/></symbol><symbol id="icon-newsletter" viewBox="0 0 18 18"><path d="m9 11.8482489 2-1.1428571v-1.7053918h-4v1.7053918zm-3-1.7142857v-2.1339632h6v2.1339632l3-1.71428574v-6.41967746h-12v6.41967746zm10-5.3839632 1.5299989.95624934c.2923814.18273835.4700011.50320827.4700011.8479983v8.44575236c0 1.1045695-.8954305 2-2 2h-14c-1.1045695 0-2-.8954305-2-2v-8.44575236c0-.34479003.1776197-.66525995.47000106-.8479983l1.52999894-.95624934v-2.75c0-.55228475.44771525-1 1-1h12c.5522847 0 1 .44771525 1 1zm0 1.17924764v3.07075236l-7 4-7-4v-3.07075236l-1 .625v8.44575236c0 .5522847.44771525 1 1 1h14c.5522847 0 1-.4477153 1-1v-8.44575236zm-10-1.92924764h6v1h-6zm-1 2h8v1h-8z" fill-rule="evenodd"/></symbol><symbol id="icon-orcid" viewBox="0 0 18 18"><path d="m9 1c4.418278 0 8 3.581722 8 8s-3.581722 8-8 8-8-3.581722-8-8 3.581722-8 8-8zm-2.90107518 5.2732337h-1.41865256v7.1712107h1.41865256zm4.55867178.02508949h-2.99247027v7.14612121h2.91062487c.7673039 0 1.4476365-.1483432 2.0410182-.445034s1.0511995-.7152915 1.3734671-1.2558144c.3222677-.540523.4833991-1.1603247.4833991-1.85942385 0-.68545815-.1602789-1.30270225-.4808414-1.85175082-.3205625-.54904856-.7707074-.97532211-1.3504481-1.27883343-.5797408-.30351132-1.2413173-.45526471-1.9847495-.45526471zm-.1892674 1.07933542c.7877654 0 1.4143875.22336734 1.8798852.67010873.4654977.44674138.698243 1.05546001.698243 1.82617415 0 .74343221-.2310402 1.34447791-.6931277 1.80315511-.4620874.4586773-1.0750688.6880124-1.8389625.6880124h-1.46810075v-4.98745039zm-5.08652545-3.71099194c-.21825533 0-.410525.08444276-.57681478.25333081-.16628977.16888806-.24943341.36245684-.24943341.58071218 0 .22345188.08314364.41961891.24943341.58850696.16628978.16888806.35855945.25333082.57681478.25333082.233845 0 .43390938-.08314364.60019916-.24943342.16628978-.16628977.24943342-.36375592.24943342-.59240436 0-.233845-.08314364-.43131115-.24943342-.59240437s-.36635416-.24163862-.60019916-.24163862z" fill-rule="evenodd"/></symbol><symbol id="icon-plus" viewBox="0 0 16 16"><path d="m2.00087166 7h4.99912834v-4.99912834c0-.55276616.44386482-1.00087166 1-1.00087166.55228475 0 1 .44463086 1 1.00087166v4.99912834h4.9991283c.5527662 0 1.0008717.44386482 1.0008717 1 0 .55228475-.4446309 1-1.0008717 1h-4.9991283v4.9991283c0 .5527662-.44386482 1.0008717-1 1.0008717-.55228475 0-1-.4446309-1-1.0008717v-4.9991283h-4.99912834c-.55276616 0-1.00087166-.44386482-1.00087166-1 0-.55228475.44463086-1 1.00087166-1z" fill-rule="evenodd"/></symbol><symbol id="icon-print" viewBox="0 0 18 18"><path d="m16.0049107 5h-14.00982141c-.54941618 0-.99508929.4467783-.99508929.99961498v6.00077002c0 .5570958.44271433.999615.99508929.999615h1.00491071v-3h12v3h1.0049107c.5494162 0 .9950893-.4467783.9950893-.999615v-6.00077002c0-.55709576-.4427143-.99961498-.9950893-.99961498zm-2.0049107-1v-2.00208688c0-.54777062-.4519464-.99791312-1.0085302-.99791312h-7.9829396c-.55661731 0-1.0085302.44910695-1.0085302.99791312v2.00208688zm1 10v2.0018986c0 1.103521-.9019504 1.9981014-2.0085302 1.9981014h-7.9829396c-1.1092806 0-2.0085302-.8867064-2.0085302-1.9981014v-2.0018986h-1.00491071c-1.10185739 0-1.99508929-.8874333-1.99508929-1.999615v-6.00077002c0-1.10435686.8926228-1.99961498 1.99508929-1.99961498h1.00491071v-2.00208688c0-1.10341695.90195036-1.99791312 2.0085302-1.99791312h7.9829396c1.1092806 0 2.0085302.89826062 2.0085302 1.99791312v2.00208688h1.0049107c1.1018574 0 1.9950893.88743329 1.9950893 1.99961498v6.00077002c0 1.1043569-.8926228 1.999615-1.9950893 1.999615zm-1-3h-10v5.0018986c0 .5546075.44702548.9981014 1.0085302.9981014h7.9829396c.5565964 0 1.0085302-.4491701 1.0085302-.9981014zm-9 1h8v1h-8zm0 2h5v1h-5zm9-5c-.5522847 0-1-.44771525-1-1s.4477153-1 1-1 1 .44771525 1 1-.4477153 1-1 1z" fill-rule="evenodd"/></symbol><symbol id="icon-search" viewBox="0 0 22 22"><path d="M21.697 20.261a1.028 1.028 0 01.01 1.448 1.034 1.034 0 01-1.448-.01l-4.267-4.267A9.812 9.811 0 010 9.812a9.812 9.811 0 1117.43 6.182zM9.812 18.222A8.41 8.41 0 109.81 1.403a8.41 8.41 0 000 16.82z" fill-rule="evenodd"/></symbol><symbol id="icon-social-facebook" viewBox="0 0 24 24"><path d="m6.00368507 20c-1.10660471 0-2.00368507-.8945138-2.00368507-1.9940603v-12.01187942c0-1.10128908.89451376-1.99406028 1.99406028-1.99406028h12.01187942c1.1012891 0 1.9940603.89451376 1.9940603 1.99406028v12.01187942c0 1.1012891-.88679 1.9940603-2.0032184 1.9940603h-2.9570132v-6.1960818h2.0797387l.3114113-2.414723h-2.39115v-1.54164807c0-.69911803.1941355-1.1755439 1.1966615-1.1755439l1.2786739-.00055875v-2.15974763l-.2339477-.02492088c-.3441234-.03134957-.9500153-.07025255-1.6293054-.07025255-1.8435726 0-3.1057323 1.12531866-3.1057323 3.19187953v1.78079225h-2.0850778v2.414723h2.0850778v6.1960818z" fill-rule="evenodd"/></symbol><symbol id="icon-social-twitter" viewBox="0 0 24 24"><path d="m18.8767135 6.87445248c.7638174-.46908424 1.351611-1.21167363 1.6250764-2.09636345-.7135248.43394112-1.50406.74870123-2.3464594.91677702-.6695189-.73342162-1.6297913-1.19486605-2.6922204-1.19486605-2.0399895 0-3.6933555 1.69603749-3.6933555 3.78628909 0 .29642457.0314329.58673729.0942985.8617704-3.06469922-.15890802-5.78835241-1.66547825-7.60988389-3.9574208-.3174714.56076194-.49978171 1.21167363-.49978171 1.90536824 0 1.31404706.65223085 2.47224203 1.64236444 3.15218497-.60350999-.0198635-1.17401554-.1925232-1.67222562-.47366811v.04583885c0 1.83355406 1.27302891 3.36609966 2.96411421 3.71294696-.31118484.0886217-.63651445.1329326-.97441718.1329326-.2357461 0-.47149219-.0229194-.69466516-.0672303.47149219 1.5065703 1.83253297 2.6036468 3.44975116 2.632678-1.2651707 1.0160946-2.85724264 1.6196394-4.5891906 1.6196394-.29861172 0-.59093688-.0152796-.88011875-.0504227 1.63450624 1.0726291 3.57548241 1.6990934 5.66104951 1.6990934 6.79263079 0 10.50641749-5.7711113 10.50641749-10.7751859l-.0094298-.48894775c.7229547-.53478659 1.3516109-1.20250585 1.8419628-1.96190282-.6632323.30100846-1.3751855.50422736-2.1217148.59590507z" fill-rule="evenodd"/></symbol><symbol id="icon-social-youtube" viewBox="0 0 24 24"><path d="m10.1415 14.3973208-.0005625-5.19318431 4.863375 2.60554491zm9.963-7.92753362c-.6845625-.73643756-1.4518125-.73990314-1.803375-.7826454-2.518875-.18714178-6.2971875-.18714178-6.2971875-.18714178-.007875 0-3.7861875 0-6.3050625.18714178-.352125.04274226-1.1188125.04620784-1.8039375.7826454-.5394375.56084773-.7149375 1.8344515-.7149375 1.8344515s-.18 1.49597903-.18 2.99138042v1.4024082c0 1.495979.18 2.9913804.18 2.9913804s.1755 1.2736038.7149375 1.8344515c.685125.7364376 1.5845625.7133337 1.9850625.7901542 1.44.1420891 6.12.1859866 6.12.1859866s3.78225-.005776 6.301125-.1929178c.3515625-.0433198 1.1188125-.0467854 1.803375-.783223.5394375-.5608477.7155-1.8344515.7155-1.8344515s.18-1.4954014.18-2.9913804v-1.4024082c0-1.49540139-.18-2.99138042-.18-2.99138042s-.1760625-1.27360377-.7155-1.8344515z" fill-rule="evenodd"/></symbol><symbol id="icon-subject-medicine" viewBox="0 0 18 18"><path d="m12.5 8h-6.5c-1.65685425 0-3 1.34314575-3 3v1c0 1.6568542 1.34314575 3 3 3h1v-2h-.5c-.82842712 0-1.5-.6715729-1.5-1.5s.67157288-1.5 1.5-1.5h1.5 2 1 2c1.6568542 0 3-1.34314575 3-3v-1c0-1.65685425-1.3431458-3-3-3h-2v2h1.5c.8284271 0 1.5.67157288 1.5 1.5s-.6715729 1.5-1.5 1.5zm-5.5-1v-1h-3.5c-1.38071187 0-2.5-1.11928813-2.5-2.5s1.11928813-2.5 2.5-2.5h1.02786405c.46573528 0 .92507448.10843528 1.34164078.31671843l1.13382424.56691212c.06026365-1.05041141.93116291-1.88363055 1.99667093-1.88363055 1.1045695 0 2 .8954305 2 2h2c2.209139 0 4 1.790861 4 4v1c0 2.209139-1.790861 4-4 4h-2v1h2c1.1045695 0 2 .8954305 2 2s-.8954305 2-2 2h-2c0 1.1045695-.8954305 2-2 2s-2-.8954305-2-2h-1c-2.209139 0-4-1.790861-4-4v-1c0-2.209139 1.790861-4 4-4zm0-2v-2.05652691c-.14564246-.03538148-.28733393-.08714006-.42229124-.15461871l-1.15541752-.57770876c-.27771087-.13885544-.583937-.21114562-.89442719-.21114562h-1.02786405c-.82842712 0-1.5.67157288-1.5 1.5s.67157288 1.5 1.5 1.5zm4 1v1h1.5c.2761424 0 .5-.22385763.5-.5s-.2238576-.5-.5-.5zm-1 1v-5c0-.55228475-.44771525-1-1-1s-1 .44771525-1 1v5zm-2 4v5c0 .5522847.44771525 1 1 1s1-.4477153 1-1v-5zm3 2v2h2c.5522847 0 1-.4477153 1-1s-.4477153-1-1-1zm-4-1v-1h-.5c-.27614237 0-.5.2238576-.5.5s.22385763.5.5.5zm-3.5-9h1c.27614237 0 .5.22385763.5.5s-.22385763.5-.5.5h-1c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5z" fill-rule="evenodd"/></symbol><symbol id="icon-success" viewBox="0 0 18 18"><path d="m9 0c4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9zm3.4860198 4.98163161-4.71802968 5.50657859-2.62834168-2.02300024c-.42862421-.36730544-1.06564993-.30775346-1.42283677.13301307-.35718685.44076653-.29927542 1.0958383.12934879 1.46314377l3.40735508 2.7323063c.42215801.3385221 1.03700951.2798252 1.38749189-.1324571l5.38450527-6.33394549c.3613513-.43716226.3096573-1.09278382-.115462-1.46437175-.4251192-.37158792-1.0626796-.31842941-1.4240309.11873285z" fill-rule="evenodd"/></symbol><symbol id="icon-table" viewBox="0 0 18 18"><path d="m16.0049107 2c1.1018574 0 1.9950893.89706013 1.9950893 2.00585866v9.98828264c0 1.1078052-.8926228 2.0058587-1.9950893 2.0058587l-4.0059107-.001.001.001h-1l-.001-.001h-5l.001.001h-1l-.001-.001-3.00391071.001c-1.10185739 0-1.99508929-.8970601-1.99508929-2.0058587v-9.98828264c0-1.10780515.8926228-2.00585866 1.99508929-2.00585866zm-11.0059107 5h-3.999v6.9941413c0 .5572961.44630695 1.0058587.99508929 1.0058587h3.00391071zm6 0h-5v8h5zm5.0059107-4h-4.0059107v3h5.001v1h-5.001v7.999l4.0059107.001c.5487152 0 .9950893-.4488783.9950893-1.0058587v-9.98828264c0-.55729607-.446307-1.00585866-.9950893-1.00585866zm-12.5049107 9c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-1c-.27614237 0-.5-.2238576-.5-.5s.22385763-.5.5-.5zm12 0c.2761424 0 .5.2238576.5.5s-.2238576.5-.5.5h-2c-.2761424 0-.5-.2238576-.5-.5s.2238576-.5.5-.5zm-6 0c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-2c-.27614237 0-.5-.2238576-.5-.5s.22385763-.5.5-.5zm-6-2c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-1c-.27614237 0-.5-.2238576-.5-.5s.22385763-.5.5-.5zm12 0c.2761424 0 .5.2238576.5.5s-.2238576.5-.5.5h-2c-.2761424 0-.5-.2238576-.5-.5s.2238576-.5.5-.5zm-6 0c.27614237 0 .5.2238576.5.5s-.22385763.5-.5.5h-2c-.27614237 0-.5-.2238576-.5-.5s.22385763-.5.5-.5zm-6-2c.27614237 0 .5.22385763.5.5s-.22385763.5-.5.5h-1c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5zm12 0c.2761424 0 .5.22385763.5.5s-.2238576.5-.5.5h-2c-.2761424 0-.5-.22385763-.5-.5s.2238576-.5.5-.5zm-6 0c.27614237 0 .5.22385763.5.5s-.22385763.5-.5.5h-2c-.27614237 0-.5-.22385763-.5-.5s.22385763-.5.5-.5zm1.499-5h-5v3h5zm-6 0h-3.00391071c-.54871518 0-.99508929.44887827-.99508929 1.00585866v1.99414134h3.999z" fill-rule="evenodd"/></symbol><symbol id="icon-tick-circle" viewBox="0 0 24 24"><path d="m12 2c5.5228475 0 10 4.4771525 10 10s-4.4771525 10-10 10-10-4.4771525-10-10 4.4771525-10 10-10zm0 1c-4.97056275 0-9 4.02943725-9 9 0 4.9705627 4.02943725 9 9 9 4.9705627 0 9-4.0294373 9-9 0-4.97056275-4.0294373-9-9-9zm4.2199868 5.36606669c.3613514-.43716226.9989118-.49032077 1.424031-.11873285s.4768133 1.02720949.115462 1.46437175l-6.093335 6.94397871c-.3622945.4128716-.9897871.4562317-1.4054264.0971157l-3.89719065-3.3672071c-.42862421-.3673054-.48653564-1.0223772-.1293488-1.4631437s.99421256-.5003185 1.42283677-.1330131l3.11097438 2.6987741z" fill-rule="evenodd"/></symbol><symbol id="icon-tick" viewBox="0 0 16 16"><path d="m6.76799012 9.21106946-3.1109744-2.58349728c-.42862421-.35161617-1.06564993-.29460792-1.42283677.12733148s-.29927541 1.04903009.1293488 1.40064626l3.91576307 3.23873978c.41034319.3393961 1.01467563.2976897 1.37450571-.0948578l6.10568327-6.660841c.3613513-.41848908.3096572-1.04610608-.115462-1.4018218-.4251192-.35571573-1.0626796-.30482786-1.424031.11366122z" fill-rule="evenodd"/></symbol><symbol id="icon-update" viewBox="0 0 18 18"><path d="m1 13v1c0 .5522847.44771525 1 1 1h14c.5522847 0 1-.4477153 1-1v-1h-1v-10h-14v10zm16-1h1v2c0 1.1045695-.8954305 2-2 2h-14c-1.1045695 0-2-.8954305-2-2v-2h1v-9c0-.55228475.44771525-1 1-1h14c.5522847 0 1 .44771525 1 1zm-1 0v1h-4.5857864l-1 1h-2.82842716l-1-1h-4.58578644v-1h5l1 1h2l1-1zm-13-8h12v7h-12zm1 1v5h10v-5zm1 1h4v1h-4zm0 2h4v1h-4z" fill-rule="evenodd"/></symbol><symbol id="icon-upload" viewBox="0 0 18 18"><path d="m10.0046024 0c.5497429 0 1.3179837.32258606 1.707238.71184039l4.5763192 4.57631922c.3931386.39313859.7118404 1.16760135.7118404 1.71431368v8.98899651c0 1.1092806-.8945138 2.0085302-1.9940603 2.0085302h-12.01187942c-1.10128908 0-1.99406028-.8926228-1.99406028-1.9950893v-14.00982141c0-1.10185739.88743329-1.99508929 1.99961498-1.99508929zm0 1h-7.00498742c-.55709576 0-.99961498.44271433-.99961498.99508929v14.00982141c0 .5500396.44491393.9950893.99406028.9950893h12.01187942c.5463747 0 .9940603-.4506622.9940603-1.0085302v-8.98899651c0-.28393444-.2150684-.80332809-.4189472-1.0072069l-4.5763192-4.57631922c-.2038461-.20384606-.718603-.41894717-1.0001312-.41894717zm-1.85576936 4.14572769c.19483374-.19483375.51177826-.19377714.70556874.00001334l2.59099082 2.59099079c.1948411.19484112.1904373.51514474.0027906.70279143-.1932998.19329987-.5046517.19237083-.7001856-.00692852l-1.74638687-1.7800176v6.14827687c0 .2717771-.23193359.492096-.5.492096-.27614237 0-.5-.216372-.5-.492096v-6.14827641l-1.74627892 1.77990922c-.1933927.1971171-.51252214.19455839-.70016883.0069117-.19329987-.19329988-.19100584-.50899493.00277731-.70277808z" fill-rule="evenodd"/></symbol><symbol id="icon-video" viewBox="0 0 18 18"><path d="m16.0049107 2c1.1018574 0 1.9950893.89706013 1.9950893 2.00585866v9.98828264c0 1.1078052-.8926228 2.0058587-1.9950893 2.0058587h-14.00982141c-1.10185739 0-1.99508929-.8970601-1.99508929-2.0058587v-9.98828264c0-1.10780515.8926228-2.00585866 1.99508929-2.00585866zm0 1h-14.00982141c-.54871518 0-.99508929.44887827-.99508929 1.00585866v9.98828264c0 .5572961.44630695 1.0058587.99508929 1.0058587h14.00982141c.5487152 0 .9950893-.4488783.9950893-1.0058587v-9.98828264c0-.55729607-.446307-1.00585866-.9950893-1.00585866zm-8.30912922 2.24944486 4.60460462 2.73982242c.9365543.55726659.9290753 1.46522435 0 2.01804082l-4.60460462 2.7398224c-.93655425.5572666-1.69578148.1645632-1.69578148-.8937585v-5.71016863c0-1.05087579.76670616-1.446575 1.69578148-.89375851zm-.67492769.96085624v5.5750128c0 .2995102-.10753745.2442517.16578928.0847713l4.58452283-2.67497259c.3050619-.17799716.3051624-.21655446 0-.39461026l-4.58452283-2.67497264c-.26630747-.15538481-.16578928-.20699944-.16578928.08477139z" fill-rule="evenodd"/></symbol><symbol id="icon-warning" viewBox="0 0 18 18"><path d="m9 11.75c.69035594 0 1.25.5596441 1.25 1.25s-.55964406 1.25-1.25 1.25-1.25-.5596441-1.25-1.25.55964406-1.25 1.25-1.25zm.41320045-7.75c.55228475 0 1.00000005.44771525 1.00000005 1l-.0034543.08304548-.3333333 4c-.043191.51829212-.47645714.91695452-.99654578.91695452h-.15973424c-.52008864 0-.95335475-.3986624-.99654576-.91695452l-.33333333-4c-.04586475-.55037702.36312325-1.03372649.91350028-1.07959124l.04148683-.00259031zm-.41320045 14c-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9 4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9z" fill-rule="evenodd"/></symbol><symbol id="icon-checklist-banner" viewBox="0 0 56.69 56.69"><path style="fill:none" d="M0 0h56.69v56.69H0z"/><clipPath id="b"><use xlink:href="#a" style="overflow:visible"/></clipPath><path d="M21.14 34.46c0-6.77 5.48-12.26 12.24-12.26s12.24 5.49 12.24 12.26-5.48 12.26-12.24 12.26c-6.76-.01-12.24-5.49-12.24-12.26zm19.33 10.66 10.23 9.22s1.21 1.09 2.3-.12l2.09-2.32s1.09-1.21-.12-2.3l-10.23-9.22m-19.29-5.92c0-4.38 3.55-7.94 7.93-7.94s7.93 3.55 7.93 7.94c0 4.38-3.55 7.94-7.93 7.94-4.38-.01-7.93-3.56-7.93-7.94zm17.58 12.99 4.14-4.81" style="clip-path:url(#b);fill:none;stroke:#01324b;stroke-width:2;stroke-linecap:round"/><path d="M8.26 9.75H28.6M8.26 15.98H28.6m-20.34 6.2h12.5m14.42-5.2V4.86s0-2.93-2.93-2.93H4.13s-2.93 0-2.93 2.93v37.57s0 2.93 2.93 2.93h15.01M8.26 9.75H28.6M8.26 15.98H28.6m-20.34 6.2h12.5" style="clip-path:url(#b);fill:none;stroke:#01324b;stroke-width:2;stroke-linecap:round;stroke-linejoin:round"/></symbol><symbol id="icon-chevron-down" viewBox="0 0 16 16"><path d="m5.58578644 3-3.29289322-3.29289322c-.39052429-.39052429-.39052429-1.02368927 0-1.41421356s1.02368927-.39052429 1.41421356 0l4 4c.39052429.39052429.39052429 1.02368927 0 1.41421356l-4 4c-.39052429.39052429-1.02368927.39052429-1.41421356 0s-.39052429-1.02368927 0-1.41421356z" fill-rule="evenodd" transform="matrix(0 1 -1 0 11 1)"/></symbol><symbol id="icon-eds-i-arrow-right-medium" viewBox="0 0 24 24"><path d="m12.728 3.293 7.98 7.99a.996.996 0 0 1 .281.561l.011.157c0 .32-.15.605-.384.788l-7.908 7.918a1 1 0 0 1-1.416-1.414L17.576 13H4a1 1 0 0 1 0-2h13.598l-6.285-6.293a1 1 0 0 1-.082-1.32l.083-.095a1 1 0 0 1 1.414.001Z"/></symbol><symbol id="icon-eds-i-chevron-down-medium" viewBox="0 0 16 16"><path d="m2.00087166 7h4.99912834v-4.99912834c0-.55276616.44386482-1.00087166 1-1.00087166.55228475 0 1 .44463086 1 1.00087166v4.99912834h4.9991283c.5527662 0 1.0008717.44386482 1.0008717 1 0 .55228475-.4446309 1-1.0008717 1h-4.9991283v4.9991283c0 .5527662-.44386482 1.0008717-1 1.0008717-.55228475 0-1-.4446309-1-1.0008717v-4.9991283h-4.99912834c-.55276616 0-1.00087166-.44386482-1.00087166-1 0-.55228475.44463086-1 1.00087166-1z" fill-rule="evenodd"/></symbol><symbol id="icon-eds-i-chevron-down-small" viewBox="0 0 16 16"><path d="M13.692 5.278a1 1 0 0 1 .03 1.414L9.103 11.51a1.491 1.491 0 0 1-2.188.019L2.278 6.692a1 1 0 0 1 1.444-1.384L8 9.771l4.278-4.463a1 1 0 0 1 1.318-.111l.096.081Z"/></symbol><symbol id="icon-eds-i-chevron-right-medium" viewBox="0 0 10 10"><path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/></symbol><symbol id="icon-eds-i-chevron-right-small" viewBox="0 0 10 10"><path d="m5.96738168 4.70639573 2.39518594-2.41447274c.37913917-.38219212.98637524-.38972225 1.35419292-.01894278.37750606.38054586.37784436.99719163-.00013556 1.37821513l-4.03074001 4.06319683c-.37758093.38062133-.98937525.38100976-1.367372-.00003075l-4.03091981-4.06337806c-.37759778-.38063832-.38381821-.99150444-.01600053-1.3622839.37750607-.38054587.98772445-.38240057 1.37006824.00302197l2.39538588 2.4146743.96295325.98624457z" fill-rule="evenodd" transform="matrix(0 -1 1 0 0 10)"/></symbol><symbol id="icon-eds-i-chevron-up-medium" viewBox="0 0 16 16"><path d="m2.00087166 7h11.99825664c.5527662 0 1.0008717.44386482 1.0008717 1 0 .55228475-.4446309 1-1.0008717 1h-11.99825664c-.55276616 0-1.00087166-.44386482-1.00087166-1 0-.55228475.44463086-1 1.00087166-1z" fill-rule="evenodd"/></symbol><symbol id="icon-eds-i-close-medium" viewBox="0 0 16 16"><path d="m2.29679575 12.2772478c-.39658757.3965876-.39438847 1.0328109-.00062148 1.4265779.39651227.3965123 1.03246768.3934888 1.42657791-.0006214l4.27724782-4.27724787 4.2772478 4.27724787c.3965876.3965875 1.0328109.3943884 1.4265779.0006214.3965123-.3965122.3934888-1.0324677-.0006214-1.4265779l-4.27724787-4.2772478 4.27724787-4.27724782c.3965875-.39658757.3943884-1.03281091.0006214-1.42657791-.3965122-.39651226-1.0324677-.39348875-1.4265779.00062148l-4.2772478 4.27724782-4.27724782-4.27724782c-.39658757-.39658757-1.03281091-.39438847-1.42657791-.00062148-.39651226.39651227-.39348875 1.03246768.00062148 1.42657791l4.27724782 4.27724782z" fill-rule="evenodd"/></symbol><symbol id="icon-eds-i-download-medium" viewBox="0 0 16 16"><path d="m12.9975267 12.999368c.5467123 0 1.0024733.4478567 1.0024733 1.000316 0 .5563109-.4488226 1.000316-1.0024733 1.000316h-9.99505341c-.54671233 0-1.00247329-.4478567-1.00247329-1.000316 0-.5563109.44882258-1.000316 1.00247329-1.000316zm-4.9975267-11.999368c.55228475 0 1 .44497754 1 .99589209v6.80214418l2.4816273-2.48241149c.3928222-.39294628 1.0219732-.4006883 1.4030652-.01947579.3911302.39125371.3914806 1.02525073-.0001404 1.41699553l-4.17620792 4.17752758c-.39120769.3913313-1.02508144.3917306-1.41671995-.0000316l-4.17639421-4.17771394c-.39122513-.39134876-.39767006-1.01940351-.01657797-1.40061601.39113012-.39125372 1.02337105-.3931606 1.41951349.00310701l2.48183446 2.48261871v-6.80214418c0-.55001601.44386482-.99589209 1-.99589209z" fill-rule="evenodd"/></symbol><symbol id="icon-eds-i-info-filled-medium" viewBox="0 0 18 18"><path d="m9 0c4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9zm0 7h-1.5l-.11662113.00672773c-.49733868.05776511-.88337887.48043643-.88337887.99327227 0 .47338693.32893365.86994729.77070917.97358929l.1126697.01968298.11662113.00672773h.5v3h-.5l-.11662113.0067277c-.42082504.0488782-.76196299.3590206-.85696816.7639815l-.01968298.1126697-.00672773.1166211.00672773.1166211c.04887817.4208251.35902055.761963.76398144.8569682l.1126697.019683.11662113.0067277h3l.1166211-.0067277c.4973387-.0577651.8833789-.4804365.8833789-.9932723 0-.4733869-.3289337-.8699473-.7707092-.9735893l-.1126697-.019683-.1166211-.0067277h-.5v-4l-.00672773-.11662113c-.04887817-.42082504-.35902055-.76196299-.76398144-.85696816l-.1126697-.01968298zm0-3.25c-.69035594 0-1.25.55964406-1.25 1.25s.55964406 1.25 1.25 1.25 1.25-.55964406 1.25-1.25-.55964406-1.25-1.25-1.25z" fill-rule="evenodd"/></symbol><symbol id="icon-eds-i-mail-medium" viewBox="0 0 24 24"><path d="m19.462 0c1.413 0 2.538 1.184 2.538 2.619v12.762c0 1.435-1.125 2.619-2.538 2.619h-16.924c-1.413 0-2.538-1.184-2.538-2.619v-12.762c0-1.435 1.125-2.619 2.538-2.619zm.538 5.158-7.378 6.258a2.549 2.549 0 0 1 -3.253-.008l-7.369-6.248v10.222c0 .353.253.619.538.619h16.924c.285 0 .538-.266.538-.619zm-.538-3.158h-16.924c-.264 0-.5.228-.534.542l8.65 7.334c.2.165.492.165.684.007l8.656-7.342-.001-.025c-.044-.3-.274-.516-.531-.516z"/></symbol><symbol id="icon-eds-i-menu-medium" viewBox="0 0 24 24"><path d="M21 4a1 1 0 0 1 0 2H3a1 1 0 1 1 0-2h18Zm-4 7a1 1 0 0 1 0 2H3a1 1 0 0 1 0-2h14Zm4 7a1 1 0 0 1 0 2H3a1 1 0 0 1 0-2h18Z"/></symbol><symbol id="icon-eds-i-search-medium" viewBox="0 0 24 24"><path d="M11 1c5.523 0 10 4.477 10 10 0 2.4-.846 4.604-2.256 6.328l3.963 3.965a1 1 0 0 1-1.414 1.414l-3.965-3.963A9.959 9.959 0 0 1 11 21C5.477 21 1 16.523 1 11S5.477 1 11 1Zm0 2a8 8 0 1 0 0 16 8 8 0 0 0 0-16Z"/></symbol><symbol id="icon-eds-i-user-single-medium" viewBox="0 0 24 24"><path d="M12 1a5 5 0 1 1 0 10 5 5 0 0 1 0-10Zm0 2a3 3 0 1 0 0 6 3 3 0 0 0 0-6Zm-.406 9.008a8.965 8.965 0 0 1 6.596 2.494A9.161 9.161 0 0 1 21 21.025V22a1 1 0 0 1-1 1H4a1 1 0 0 1-1-1v-.985c.05-4.825 3.815-8.777 8.594-9.007Zm.39 1.992-.299.006c-3.63.175-6.518 3.127-6.678 6.775L5 21h13.998l-.009-.268a7.157 7.157 0 0 0-1.97-4.573l-.214-.213A6.967 6.967 0 0 0 11.984 14Z"/></symbol><symbol id="icon-eds-i-warning-filled-medium" viewBox="0 0 18 18"><path d="m9 11.75c.69035594 0 1.25.5596441 1.25 1.25s-.55964406 1.25-1.25 1.25-1.25-.5596441-1.25-1.25.55964406-1.25 1.25-1.25zm.41320045-7.75c.55228475 0 1.00000005.44771525 1.00000005 1l-.0034543.08304548-.3333333 4c-.043191.51829212-.47645714.91695452-.99654578.91695452h-.15973424c-.52008864 0-.95335475-.3986624-.99654576-.91695452l-.33333333-4c-.04586475-.55037702.36312325-1.03372649.91350028-1.07959124l.04148683-.00259031zm-.41320045 14c-4.97056275 0-9-4.0294373-9-9 0-4.97056275 4.02943725-9 9-9 4.9705627 0 9 4.02943725 9 9 0 4.9705627-4.0294373 9-9 9z" fill-rule="evenodd"/></symbol><symbol id="icon-expand-image" viewBox="0 0 18 18"><path d="m7.49754099 11.9178212c.38955542-.3895554.38761957-1.0207846-.00290473-1.4113089-.39324695-.3932469-1.02238878-.3918247-1.41130883-.0029047l-4.10273549 4.1027355.00055454-3.5103985c.00008852-.5603185-.44832171-1.006032-1.00155062-1.0059446-.53903074.0000852-.97857527.4487442-.97866268 1.0021075l-.00093318 5.9072465c-.00008751.553948.44841131 1.001882 1.00174994 1.0017946l5.906983-.0009331c.5539233-.0000875 1.00197907-.4486389 1.00206646-1.0018679.00008515-.5390307-.45026621-.9784332-1.00588841-.9783454l-3.51010549.0005545zm3.00571741-5.83449376c-.3895554.38955541-.3876196 1.02078454.0029047 1.41130883.393247.39324696 1.0223888.39182478 1.4113089.00290473l4.1027355-4.10273549-.0005546 3.5103985c-.0000885.56031852.4483217 1.006032 1.0015506 1.00594461.5390308-.00008516.9785753-.44874418.9786627-1.00210749l.0009332-5.9072465c.0000875-.553948-.4484113-1.00188204-1.0017499-1.00179463l-5.906983.00093313c-.5539233.00008751-1.0019791.44863892-1.0020665 1.00186784-.0000852.53903074.4502662.97843325 1.0058884.97834547l3.5101055-.00055449z" fill-rule="evenodd"/></symbol><symbol id="icon-github" viewBox="0 0 100 100"><path fill-rule="evenodd" clip-rule="evenodd" d="M48.854 0C21.839 0 0 22 0 49.217c0 21.756 13.993 40.172 33.405 46.69 2.427.49 3.316-1.059 3.316-2.362 0-1.141-.08-5.052-.08-9.127-13.59 2.934-16.42-5.867-16.42-5.867-2.184-5.704-5.42-7.17-5.42-7.17-4.448-3.015.324-3.015.324-3.015 4.934.326 7.523 5.052 7.523 5.052 4.367 7.496 11.404 5.378 14.235 4.074.404-3.178 1.699-5.378 3.074-6.6-10.839-1.141-22.243-5.378-22.243-24.283 0-5.378 1.94-9.778 5.014-13.2-.485-1.222-2.184-6.275.486-13.038 0 0 4.125-1.304 13.426 5.052a46.97 46.97 0 0 1 12.214-1.63c4.125 0 8.33.571 12.213 1.63 9.302-6.356 13.427-5.052 13.427-5.052 2.67 6.763.97 11.816.485 13.038 3.155 3.422 5.015 7.822 5.015 13.2 0 18.905-11.404 23.06-22.324 24.283 1.78 1.548 3.316 4.481 3.316 9.126 0 6.6-.08 11.897-.08 13.526 0 1.304.89 2.853 3.316 2.364 19.412-6.52 33.405-24.935 33.405-46.691C97.707 22 75.788 0 48.854 0z"/></symbol><symbol id="icon-springer-arrow-left"><path d="M15 7a1 1 0 000-2H3.385l2.482-2.482a.994.994 0 00.02-1.403 1.001 1.001 0 00-1.417 0L.294 5.292a1.001 1.001 0 000 1.416l4.176 4.177a.991.991 0 001.4.016 1 1 0 00-.003-1.42L3.385 7H15z"/></symbol><symbol id="icon-springer-arrow-right"><path d="M1 7a1 1 0 010-2h11.615l-2.482-2.482a.994.994 0 01-.02-1.403 1.001 1.001 0 011.417 0l4.176 4.177a1.001 1.001 0 010 1.416l-4.176 4.177a.991.991 0 01-1.4.016 1 1 0 01.003-1.42L12.615 7H1z"/></symbol><symbol id="icon-submit-open" viewBox="0 0 16 17"><path d="M12 0c1.10457 0 2 .895431 2 2v5c0 .276142-.223858.5-.5.5S13 7.276142 13 7V2c0-.512836-.38604-.935507-.883379-.993272L12 1H6v3c0 1.10457-.89543 2-2 2H1v8c0 .512836.38604.935507.883379.993272L2 15h6.5c.276142 0 .5.223858.5.5s-.223858.5-.5.5H2c-1.104569 0-2-.89543-2-2V5.828427c0-.530433.210714-1.039141.585786-1.414213L4.414214.585786C4.789286.210714 5.297994 0 5.828427 0H12Zm3.41 11.14c.250899.250899.250274.659726 0 .91-.242954.242954-.649606.245216-.9-.01l-1.863671-1.900337.001043 5.869492c0 .356992-.289839.637138-.647372.637138-.347077 0-.647371-.285256-.647371-.637138l-.001043-5.869492L9.5 12.04c-.253166.258042-.649726.260274-.9.01-.242954-.242954-.252269-.657731 0-.91l2.942184-2.951303c.250908-.250909.66127-.252277.91353-.000017L15.41 11.14ZM5 1.413 1.413 5H4c.552285 0 1-.447715 1-1V1.413ZM11 3c.276142 0 .5.223858.5.5s-.223858.5-.5.5H7.5c-.276142 0-.5-.223858-.5-.5s.223858-.5.5-.5H11Zm0 2c.276142 0 .5.223858.5.5s-.223858.5-.5.5H7.5c-.276142 0-.5-.223858-.5-.5s.223858-.5.5-.5H11Z" fill-rule="nonzero"/></symbol></svg> </div> </footer> <div class="c-site-messages message u-hide u-hide-print c-site-messages--nature-briefing c-site-messages--nature-briefing-email-variant c-site-messages--nature-briefing-redesign-2020 sans-serif " data-component-id="nature-briefing-banner" data-component-expirydays="30" data-component-trigger-scroll-percentage="15" data-track="in-view" data-track-action="in-view" data-track-category="nature briefing" data-track-label="Briefing banner visible: Flagship"> <div class="c-site-messages__banner-large"> <div class="c-site-messages__close-container"> <button class="c-site-messages__close" data-track="click" data-track-category="nature briefing" data-track-label="Briefing banner dismiss: Flagship"> <svg width="25px" height="25px" focusable="false" aria-hidden="true" viewBox="0 0 25 25" version="1.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"> <title>Close banner</title> <defs></defs> <g stroke="none" stroke-width="1" fill="none" fill-rule="evenodd"> <rect opacity="0" x="0" y="0" width="25" height="25"></rect> <path d="M6.29679575,16.2772478 C5.90020818,16.6738354 5.90240728,17.3100587 6.29617427,17.7038257 C6.69268654,18.100338 7.32864195,18.0973145 7.72275218,17.7032043 L12,13.4259564 L16.2772478,17.7032043 C16.6738354,18.0997918 17.3100587,18.0975927 17.7038257,17.7038257 C18.100338,17.3073135 18.0973145,16.671358 17.7032043,16.2772478 L13.4259564,12 L17.7032043,7.72275218 C18.0997918,7.32616461 18.0975927,6.68994127 17.7038257,6.29617427 C17.3073135,5.89966201 16.671358,5.90268552 16.2772478,6.29679575 L12,10.5740436 L7.72275218,6.29679575 C7.32616461,5.90020818 6.68994127,5.90240728 6.29617427,6.29617427 C5.89966201,6.69268654 5.90268552,7.32864195 6.29679575,7.72275218 L10.5740436,12 L6.29679575,16.2772478 Z" fill="#ffffff"></path> </g> </svg> <span class="visually-hidden">Close</span> </button> </div> <div class="c-site-messages__form-container"> <div class="grid grid-12 last"> <div class="grid grid-4"> <img alt="Nature Briefing" src="/static/images/logos/nature-briefing-logo-n150-white-d81c9da3ec.svg" width="250" height="40"> <p class="c-site-messages--nature-briefing__strapline extra-tight-line-height">Sign up for the <em>Nature Briefing</em> newsletter — what matters in science, free to your inbox daily.</p> </div> <div class="grid grid-8 last"> <form action="https://www.nature.com/briefing/briefing" method="post" data-location="banner" data-track="signup_nature_briefing_banner" data-track-action="transmit-form" data-track-category="nature briefing" data-track-label="Briefing banner submit: Flagship"> <input id="briefing-banner-signup-form-input-track-originReferralPoint" type="hidden" name="track_originReferralPoint" value="MainBriefingBanner"> <input id="briefing-banner-signup-form-input-track-formType" type="hidden" name="track_formType" value="DirectEmailBanner"> <input type="hidden" value="false" name="gdpr_tick" id="gdpr_tick_banner"> <input type="hidden" value="false" name="marketing" id="marketing_input_banner"> <input type="hidden" value="false" name="marketing_tick" id="marketing_tick_banner"> <input type="hidden" value="MainBriefingBanner" name="brieferEntryPoint" id="brieferEntryPoint_banner"> <label class="nature-briefing-banner__email-label" for="emailAddress">Email address</label> <div class="nature-briefing-banner__email-wrapper"> <input class="nature-briefing-banner__email-input box-sizing text14" type="email" id="emailAddress" name="emailAddress" value="" placeholder="e.g. jo.smith@university.ac.uk" required data-test-element="briefing-emailbanner-email-input"> <input type="hidden" value="true" name="N:nature_briefing_daily" id="defaultNewsletter_banner"> <button type="submit" class="nature-briefing-banner__submit-button box-sizing text14" data-test-element="briefing-emailbanner-signup-button">Sign up</button> </div> <div class="nature-briefing-banner__checkbox-wrapper grid grid-12 last"> <input class="nature-briefing-banner__checkbox-checkbox" id="gdpr-briefing-banner-checkbox" type="checkbox" name="gdpr" value="true" data-test-element="briefing-emailbanner-gdpr-checkbox" required> <label class="nature-briefing-banner__checkbox-label box-sizing text13 sans-serif block tighten-line-height" for="gdpr-briefing-banner-checkbox">I agree my information will be processed in accordance with the <em>Nature</em> and Springer Nature Limited <a href="https://www.nature.com/info/privacy">Privacy Policy</a>.</label> </div> </form> </div> </div> </div> </div> <div class="c-site-messages__banner-small"> <div class="c-site-messages__close-container"> <button class="c-site-messages__close" data-track="click" data-track-category="nature briefing" data-track-label="Briefing banner dismiss: Flagship"> <svg width="25px" height="25px" focusable="false" aria-hidden="true" viewBox="0 0 25 25" version="1.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"> <title>Close banner</title> <defs></defs> <g stroke="none" stroke-width="1" fill="none" fill-rule="evenodd"> <rect opacity="0" x="0" y="0" width="25" height="25"></rect> <path d="M6.29679575,16.2772478 C5.90020818,16.6738354 5.90240728,17.3100587 6.29617427,17.7038257 C6.69268654,18.100338 7.32864195,18.0973145 7.72275218,17.7032043 L12,13.4259564 L16.2772478,17.7032043 C16.6738354,18.0997918 17.3100587,18.0975927 17.7038257,17.7038257 C18.100338,17.3073135 18.0973145,16.671358 17.7032043,16.2772478 L13.4259564,12 L17.7032043,7.72275218 C18.0997918,7.32616461 18.0975927,6.68994127 17.7038257,6.29617427 C17.3073135,5.89966201 16.671358,5.90268552 16.2772478,6.29679575 L12,10.5740436 L7.72275218,6.29679575 C7.32616461,5.90020818 6.68994127,5.90240728 6.29617427,6.29617427 C5.89966201,6.69268654 5.90268552,7.32864195 6.29679575,7.72275218 L10.5740436,12 L6.29679575,16.2772478 Z" fill="#ffffff"></path> </g> </svg> <span class="visually-hidden">Close</span> </button> </div> <div class="c-site-messages__content text14"> <span class="c-site-messages--nature-briefing__strapline strong">Get the most important science stories of the day, free in your inbox.</span> <a class="nature-briefing__link text14 sans-serif" data-track="click" data-track-category="nature briefing" data-track-label="Small-screen banner CTA to site" data-test-element="briefing-banner-link" target="_blank" rel="noreferrer noopener" href="https://www.nature.com/briefing/signup/?brieferEntryPoint=MainBriefingBanner">Sign up for Nature Briefing </a> </div> </div> </div> <noscript> <img hidden src="https://verify.nature.com/verify/nature.png" width="0" height="0" style="display: none" alt=""> </noscript> <script src="//content.readcube.com/ping?doi=10.1038/s41467-023-43718-w&format=js&last_modified=2023-12-06" async></script> </body> </html>