Assessing the Antimicrobial Activity of Chitosan Nanoparticles by Fluorescence-Labeling

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Activity of Chitosan Nanoparticles by Fluorescence-Labeling</title> <meta name="description" content="Assessing the Antimicrobial Activity of Chitosan Nanoparticles by Fluorescence-Labeling"> <meta name="keywords" content="chitosan nanoparticles, dynamic light scattering, zeta potential, confocal microscopy, antibacterial activity"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" 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</div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Assessing the Antimicrobial Activity of Chitosan Nanoparticles by Fluorescence-Labeling</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Laidson%20P.%20Gomes">Laidson P. Gomes</a>, <a href="https://publications.waset.org/abstracts/search?q=Cristina%20T.%20Andrade"> Cristina T. Andrade</a>, <a href="https://publications.waset.org/abstracts/search?q=Eduardo%20M.%20Del%20Aguila"> Eduardo M. Del Aguila</a>, <a href="https://publications.waset.org/abstracts/search?q=Cameron%20Alexander"> Cameron Alexander</a>, <a href="https://publications.waset.org/abstracts/search?q=V%C3%A2nia%20M.%20F.%20Paschoalin"> V芒nia M. F. Paschoalin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chitosan is a natural polysaccharide prepared by the N-deacetylation of chitin. In this study, the physicochemical and antibacterial properties of chitosan nanoparticles, produced by ultrasound irradiation, were evaluated. The physicochemical properties of the nanoparticles were determined by dynamic light scattering and zeta potential analysis. Chitosan nanoparticles inhibited the growth of <em>E. coli</em>. The minimum inhibitory concentration (MIC) values were lower than 0.5 mg/mL, and the minimum bactericidal concentration (MBC) values were similar or higher than MIC values. Confocal laser scanning micrographs (CLSM) were used to observe the interaction between <em>E. coli </em>suspensions mixed with FITC-labeled chitosan polymers and nanoparticles. <iframe src="https://publications.waset.org/abstracts/84752.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chitosan%20nanoparticles" title="chitosan nanoparticles">chitosan nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=dynamic%20light%20scattering" title=" dynamic light scattering"> dynamic light scattering</a>, <a href="https://publications.waset.org/abstracts/search?q=zeta%20potential" title=" zeta potential"> zeta potential</a>, <a href="https://publications.waset.org/abstracts/search?q=confocal%20microscopy" title=" confocal microscopy"> confocal microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=antibacterial%20activity" title=" antibacterial activity"> antibacterial activity</a> </p> <a href="https://publications.waset.org/abstracts/84752/assessing-the-antimicrobial-activity-of-chitosan-nanoparticles-by-fluorescence-labeling" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84752.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">501</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates 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Assessing the Antimicrobial Activity of Chitosan Nanoparticles by Fluorescence-Labeling