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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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We provide options for all experience levels when it comes to designing your flow cytometry panel.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsystextimage_8b9"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.full.high.jpg/1688676978392.jpg' media="(min-width: 1px)"><img class='textimage-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.full.high.jpg/1688676978392.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.320.high.jpg/1688676978392.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.480.high.jpg/1688676978392.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.320.medium.jpg/1688676978392.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.620.high.jpg/1688676978392.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.320.low.jpg/1688676978392.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_8b9/image.img.480.medium.jpg/1688676978392.jpg" alt='We provide free flow cytometry panel design service for all customers' title='Free panel design service'></picture></div><style> #cq-textimage-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsystextimage_8b9 { max-width: 100%; } #cq-textimage-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsystextimage_8b9 .image-container { max-width: 100%; width: px; height: px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3>Getting started with our <b>free panel design service</b></h3><p>We have a team of technical support scientists available to help you. Our team provides this<b> Panel Design Service</b> for those who want help with building their panel every step of the way.</p><p><a class="btn btn-featured" href="/sg/en/home/global/forms/life-science/help-flow-cytometry-immunophenotyping-panel.html">Request Panel Design with a Scientist</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsystextimage_51c7"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.full.high.jpg/1688676978426.jpg' media="(min-width: 1px)"><img class='textimage-cover' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.full.high.jpg/1688676978426.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.320.high.jpg/1688676978426.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.480.high.jpg/1688676978426.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.320.medium.jpg/1688676978426.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.620.high.jpg/1688676978426.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.320.low.jpg/1688676978426.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/textimage_51c7/image.img.480.medium.jpg/1688676978426.jpg" alt='We provide free flow cytometry panel builder tool for self-panel design' title='Free panel builder webtool'></picture></div><style> #cq-textimage-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsystextimage_51c7 { max-width: 100%; } #cq-textimage-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsystextimage_51c7 .image-container { max-width: 100%; width: px; height: px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3>Getting started with the <b>flow cytometry panel builder tool</b></h3><p>Choosing the optimal combination of fluorochromes can be simplified with a guided method. The <b>Flow Panel Builder</b> offers a curated and customizable approach to the panel building process.<br></p><p><a class="btn btn-featured" href="/order/panel-builder/#!/">Go to Panel Builder Tool</a></p></div></div><div class="clearfix"></div></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><div class="table-component-wrapper"><table border="1" cellspacing="0" cellpadding="0" width="623" class="rte-table" id="table--1477728838"><tbody><tr><th> </th><th><b><a href="/sg/en/home/global/forms/life-science/help-flow-cytometry-immunophenotyping-panel.html">Panel Design Service</a></b></th><th><b><a href="/order/panel-builder/#!/">Panel Builder Tool</a></b></th></tr><tr><td>What</td><td>Take advantage of expert insight when: <ul><li>Designing a new panel<br></li><li>Troubleshooting an existing panel<br></li><li>Revising and optimizing an existing panel with new markers</li></ul></td><td>Intuitive interface that: <ul><li>Organizes fluorochrome marker selections</li><li>Reports predicted spillover values</li><li>Shows fluorescence spectra according to your cytometer configuration</li></ul></td></tr><tr><td>Target users</td><td>All flow cytometry users (all users—from naïve to experienced—are welcome to use)</td><td>All flow cytometry users confident in their ability to design/optimize panels on their own</td></tr><tr><td>Time frame</td><td>Initial response: within 24 hours</td><td>Instant</td></tr><tr><td>Who</td><td>Live scientists with panel design expertise</td><td>Webtool</td></tr><tr><td>Product purchase</td><td colspan="2" style="text-align: center;">Not needed</td></tr><tr><td>Cost</td><td colspan="2" style="text-align: center;">FREE (multiple-time usage, no catch)</td></tr><tr><td>What to expect</td><td>Panel design, panel optimization, complete end-to-end service with final panel design; review with a minimum of 2 experts</td><td>Design panels in 5 easy steps<br><br></td></tr><tr><td>Mode of communication</td><td>First, complete the <a href="/sg/en/home/global/forms/life-science/help-flow-cytometry-immunophenotyping-panel.html">panel design service request form</a>; our experts will then contact you by email/phone as needed</td><td>Saved panels can still be used to communicate with the experts for “Free Panel Design Service”</td></tr></tbody></table></div></div><div class="text parbase section"><ul><li><a href="#steps">Get your flow cytometry panels designed in 4 easy steps</a></li><li><a href="#berencia">Be the spark story - Berencia</a></li><li><a href="#tool">Getting started with the flow cytometry panel builder tool</a></li><li><a href="#tips-tricks">Tips and Tricks for Panel Design</a></li></ul></div><div class="heading section"><h2 id="steps"><br>Get your flow cytometry panels designed in 4 easy steps </h2></div><div class="image parbase section"><div id="cq-image-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsysimage_cc2"><a class="image-enlarge-link" data-lightbox="image_cc2" href="/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/_jcr_content/MainParsys/image_cc2/backgroundimg.img.jpg/1688676978495.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.320.low.jpg/1688676978477.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.320.medium.jpg/1688676978477.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.480.medium.jpg/1688676978477.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.620.high.jpg/1688676978477.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.full.high.jpg/1688676978477.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.full.high.jpg/1688676978477.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.320.high.jpg/1688676978477.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.480.high.jpg/1688676978477.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.320.medium.jpg/1688676978477.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.620.high.jpg/1688676978477.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.320.low.jpg/1688676978477.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/image_cc2/foregroundimg.img.480.medium.jpg/1688676978477.jpg" alt='Request panel design service in 4 easy steps' title='Panel Design request in 4 steps'></picture><style> #cq-image-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsysimage_cc2 img{ width:100%; max-width: 1200px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsysimage_cc2 .image-contain" + "," + "#cq-image-jsp-"+"9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsysimage_cc2 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class="icon-zoom-in"> </span>Click image to enlarge</small></div></div></div><div class="heading section"><h2 id="berencia"> Be the spark story - Berencia </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="text parbase section"><p>Inspiring flow cytometry scientists, we create new multicolor panels at no cost for you.</p></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="brightcoveplayer section"><div data-video="cdd459e9c084"><script type="text/javascript"> var loadEME = true; 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The Invitrogen Flow Panel Builder offers a customizable panel building process to fit your flow cytometry experimental needs, whatever your experience level.</p></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="brightcoveplayer section"><div data-video="6ff21a0e9da6"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-6ff21a0e9da6', 10); </script></div><div class="video-container video-single"><div id="span-video-6ff21a0e9da6" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-6ff21a0e9da6" class="brightcove-container"><script>document.createElement('video')</script><video id="video-6ff21a0e9da6" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6206990159001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder", "description": "The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/32a566b4-49b1-4022-a443-dbea3a48221d/89b7faf9-c831-4c32-8023-e1753d74a709/160x90/match/image.jpg"], "uploadDate": "2020-11-04T09:45:33.225Z", "duration": "PT8M21.269S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6206990159001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6206990159001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6206990159001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="heading section"><h3> Video: how to design spectral flow cytometry experiments </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="text parbase section"><p>Watch the video to create a panel for your spectral instrument</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><h4><a id="fluorophores-table"></a>List of available fluorophores based on their usage, benefits, and intended applications with <a href="/sg/en/home/life-science/cell-analysis/fluorophores.html">Flurophore Selection Guide</a>.</h4></div><div class="text parbase section"><h4>See individual excitation and emission plots on <a href="/sg/en/home/life-science/cell-analysis/fluorophores/guide-fluorescence-spectraviewer.html">the Invitrogen SpectraViewer</a>.</h4></div><div class="heading section"><h3 id="tips-tricks"> Tips and Tricks for Panel Design </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="brightcoveplayer section"><div data-video="38a2c96eca28"><script type="text/javascript"> var loadEME = true; 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var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="heading section"><h4> Tip 1: Minimize spill-over by using the correct and separate channel </h4></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="accordion section"><div class="accordion" id="17ebe594a8ed4d7eb2982f7beba31006" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#17ebe594a8ed4d7eb2982f7beba31006" href="#accordion1"><span class="caret"></span> Transcript </a></div><div class="accordion-body collapse " id="accordion1"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>Any time you have markers that you know will be co-expressed on your cells of interest, make sure to space them out into separate channels. If you will need to use any adjacent channels, that's where you would put any markers that are mutually exclusive so that they'll still be easy to distinguish.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#17ebe594a8ed4d7eb2982f7beba31006').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#17ebe594a8ed4d7eb2982f7beba31006').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="brightcoveplayer section"><div data-video="4616bf09802c"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-4616bf09802c', 10); </script></div><div class="video-container video-single"><div id="span-video-4616bf09802c" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-4616bf09802c" class="brightcove-container"><script>document.createElement('video')</script><video id="video-4616bf09802c" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6050082192001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Pro tips to staining intracellular targets for flow cytometry", "description": "Learn about buffers and what role they play in fixing cells and permeabilizing membranes", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/6d751a65-9b46-4eb1-9ad4-0d3c87e18cb9/50f3ec49-210b-4923-8a6e-a7d0634e60ec/160x90/match/image.jpg"], "uploadDate": "2019-06-19T17:46:44.350Z", "duration": "PT39.104S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6050082192001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050082192001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050082192001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="heading section"><h4> Tip 2: Intracellular targets need special buffer for fixation and permeabilization for staining </h4></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="accordion section"><div class="accordion" id="3e7289bbb8c04b84bbc6f21b061b4e9d" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#3e7289bbb8c04b84bbc6f21b061b4e9d" href="#accordion2"><span class="caret"></span> Transcript </a></div><div class="accordion-body collapse " id="accordion2"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>You'll also want to keep in mind the buffer that you're using to fix and permeabilize your cells, as we have several options. When you're looking at cytoplasmic targets, what the buffer is appropriate may not be the same as when you're looking at nuclear targets, because you want to make sure that you still have access to your antigens without over-fixing your epitopes.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#3e7289bbb8c04b84bbc6f21b061b4e9d').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#3e7289bbb8c04b84bbc6f21b061b4e9d').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="brightcoveplayer section"><div data-video="92c20383e9d8"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-92c20383e9d8', 10); </script></div><div class="video-container video-single"><div id="span-video-92c20383e9d8" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-92c20383e9d8" class="brightcove-container"><script>document.createElement('video')</script><video id="video-92c20383e9d8" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6050075509001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Why use a viability dye?", "description": "A tip to finding your live cell population by incorporating a viability dye", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/3bf8ab94-696e-4dd0-8617-df0470609248/e6948b9f-0b84-4f7d-8d6f-9a90037cbd83/160x90/match/image.jpg"], "uploadDate": "2019-06-19T17:25:52.311Z", "duration": "PT39.104S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6050075509001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050075509001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050075509001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="heading section"><h4> Tip 3: Viability dyes are required to find live cells </h4></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="accordion section"><div class="accordion" id="0a9cef13d04849f599e0eed93f06e92f" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#0a9cef13d04849f599e0eed93f06e92f" href="#accordion3"><span class="caret"></span> Transcript </a></div><div class="accordion-body collapse " id="accordion3"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>A third tip I wanted to share with you is to always include a viability dye in your staining panel. This will help eliminate any false positives that are caused by dead cells or debris, because those can be sticky. You have a lot of options for choosing a viability dye, so you don't need to design your panel around them. You can build out the rest of your panel and optimize your core markers, and then fit in a viability dye in an empty channel.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#0a9cef13d04849f599e0eed93f06e92f').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#0a9cef13d04849f599e0eed93f06e92f').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="brightcoveplayer section"><div data-video="c36924879db2"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-c36924879db2', 10); </script></div><div class="video-container video-single"><div id="span-video-c36924879db2" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-c36924879db2" class="brightcove-container"><script>document.createElement('video')</script><video id="video-c36924879db2" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6050083126001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Tips on picking dyes for your flow cytometry experiment", "description": "Tip to find your low expressing markers for a flow cytometry experiment", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/d8cb8800-20e1-44f5-8f3c-f8e5a93e2a72/867fab31-0120-4213-a5cb-da704abe018e/160x90/match/image.jpg"], "uploadDate": "2019-06-19T17:37:35.373Z", "duration": "PT30.677S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6050083126001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050083126001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050083126001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="heading section"><h4> Tip 4: Save your bright fluorochromes for dim targets </h4></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="accordion section"><div class="accordion" id="6e94573deabe454db25dd3a54ad63012" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#6e94573deabe454db25dd3a54ad63012" href="#accordion4"><span class="caret"></span> Transcript </a></div><div class="accordion-body collapse " id="accordion4"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>As you're building out your basic panel and you want to incorporate some more antigens, make sure you're keeping the density of your antigen expression in mind. So if you have antigens with low or unknown expression, those would be ones that you want to assign to your brightest dyes, such as PE or APC.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#6e94573deabe454db25dd3a54ad63012').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#6e94573deabe454db25dd3a54ad63012').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="brightcoveplayer section"><div data-video="33c7b8e53ca1"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-33c7b8e53ca1', 10); </script></div><div class="video-container video-single"><div id="span-video-33c7b8e53ca1" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-33c7b8e53ca1" class="brightcove-container"><script>document.createElement('video')</script><video id="video-33c7b8e53ca1" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6050068864001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "What's a dump channel and why use it?", "description": "This video describes the benefit of pooling your negative markers together in one channel", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/2957bfd8-fc4a-462d-87ef-2b1a5ec98af0/09d1f6d8-0d68-4b78-b431-2a6fdf978516/160x90/match/image.jpg"], "uploadDate": "2019-06-19T17:10:03.074Z", "duration": "PT40.107S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6050068864001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050068864001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6050068864001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="heading section"><h4> Tip 5: Try to combine negative markers in one channel (dump channel) to save space on your panel </h4></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="accordion section"><div class="accordion" id="b1b1efc39a774c85b87f2a581b3e7452" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#b1b1efc39a774c85b87f2a581b3e7452" href="#accordion5"><span class="caret"></span> Transcript </a></div><div class="accordion-body collapse " id="accordion5"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>A helpful trick when you want to exclude a lot of cell types at once without having to suck up multiple channels for that would be to use a dump channel. This is where you're placing all the antibodies that identify your cells that are not of interest into the same channel with the same fluorochrome, and then those can be easily gated out and all of the cells negative for the dump channel would be those that you use for your analysis going forward.</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#b1b1efc39a774c85b87f2a581b3e7452').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#b1b1efc39a774c85b87f2a581b3e7452').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsyswell_db04uiparimage_9f97"><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-learning-center/immunology-at-work.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.320.high.jpg/1688676978961.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.480.high.jpg/1688676978961.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.full.high.jpg/1688676978961.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.320.high.jpg/1688676978961.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.480.high.jpg/1688676978961.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.320.medium.jpg/1688676978961.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.620.high.jpg/1688676978961.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.320.low.jpg/1688676978961.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_db04/uipar/image_9f97/foregroundimg.img.480.medium.jpg/1688676978961.jpg" alt='Promotional image for Immunology at Work Resource Center' title='Immunology at work'></picture><style> #cq-image-jsp-9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsyswell_db04uiparimage_9f97 img{ width:100%; max-width: 550px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsyswell_db04uiparimage_9f97 .image-contain" + "," + "#cq-image-jsp-"+"9e2eca3e-4b41-4744-9a1b-ed832db99bc0MainParsyswell_db04uiparimage_9f97 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><h4>Immunology at work</h4><p>Learn immune cell type markers and protocols. The Invitrogen Immunology at Work Resource Center is a learning center with technical content designed for new and experienced life scientists alike exploring the field of immunology.</p><p><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-learning-center/immunology-at-work.html" class="cta-link">Get started on your experiments</a></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><h2> Resources </h2></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><h3>Fluorophores excited by laser</h3><ul class="the-icons"><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/primary-antibodies-violet-laser.html">Primary antibodies for the violet laser</a></li><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/primary-antibodies-blue-laser.html">Primary antibodies for the blue laser</a></li><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/primary-antibodies-red-laser.html">Primary antibodies for the red laser</a></li><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/primary-antibodies-green-yellow-lasers.html">Primary antibodies for green and yellow lasers</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="A3"><div class="text parbase section"><h3>Related products</h3><ul class="the-icons"><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/phospho-specific-antibodies-flow-cytometry.html">Phospho-specific antibodies for flow cytometry</a></li><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometry-assays-reagents.html">Flow cytometry assays & reagents</a></li><li><a href="/sg/en/home/life-science/antibodies/antibody-labeling.html">Antibody and protein labeling kits</a></li><li><a href="/sg/en/home/global/forms/life-science/ebio-custom-quote-request.html">Custom antibody service</a></li><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/spectral-flow-cytometry-assays-reagents.html">Spectral flow cytometry signature guide</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="A4"><div class="text parbase section"><h3>Flow cytometers</h3><ul class="the-icons"><li><a href="/sg/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/attune-nxt-flow-cytometer.html">Attune NxT Flow Cytometer</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><h2> Support </h2></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><ul class="the-icons"><li><a href="/sg/en/home/global/forms/life-science/help-flow-cytometry-immunophenotyping-panel.html">Request help from one of our specialists to build a panel</a></li><li><a href="/sg/en/home/technical-resources/contact-us.html">Contact us</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="text parbase section"><h3>Learning</h3></div></div><div container-id="cq-colctrl-lt0" comp-id="B4"><div class="brightcoveplayer section"><div data-video="1fb4a46bd2e1"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-1fb4a46bd2e1', 10); </script><div id="1fb4a46bd2e1-Modal" class="modal modal-video" tabindex="-1" role="dialog" aria-labelledby="1fb4a46bd2e1-Modal-Label" aria-hidden="true" style="display: none;"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">x</button></div><div data-video-modal-body="1fb4a46bd2e1" class="modal-body"></div></div><script> $(document).ready(function(){ $('#1fb4a46bd2e1').bind('click', function () { $.ajax({url: '/content/lifetech/ipac/en-sg/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_72ba/uipar/brightcoveplayer_cc7.ajax.html?cached=false', data: { account: '3663210762001', videoPlayer: '6153900853001', playerPath: '' }, success: function(result) { $('[data-video-modal-body*=1fb4a46bd2e1]').html(result); }}); }); $('#1fb4a46bd2e1-Modal').bind('show', function () { $.ajax({url: '/content/lifetech/ipac/en-sg/home/life-science/cell-analysis/flow-cytometry/antibodies-for-flow-cytometry/flow-cytometry-panel-builder/jcr:content/MainParsys/well_72ba/uipar/brightcoveplayer_cc7.ajax.html?cached=false', data: { account: '3663210762001', videoPlayer: '6153900853001', playerPath: '' }, success: function(result) { $('[data-video-modal-body*=1fb4a46bd2e1]').html(result); }}); }); }); $('#1fb4a46bd2e1-Modal').bind('hide', function () { var htmlVideoId = $('[data-video-id*=6153900853001]').attr('id'); try { videojs(htmlVideoId).dispose(); } catch (e) { console.log('Unable to destroy videojs player.'); } $('[data-video-modal-body*=1fb4a46bd2e1]').html(''); }); </script></div><button id="1fb4a46bd2e1" type="button" class="btn-link normal-whitespace" data-toggle="modal" data-target="#1fb4a46bd2e1-Modal">Webinar: Panel Building Tools and Techniques for Flow Cytometry</button><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Panel Building Tools and Techniques for Flow Cytometry", "description": "Learn about: Panel design, compensation beads, autofluorescence, back-gating, methods and tools to help easier use of your flow cytometer", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/jit/3663210762001/192c47b3-af0f-4d06-a0e9-8e41ad725fc8/main/160x90/28m54s272ms/match/image.jpg"], "uploadDate": "2020-05-04T01:32:25.781Z", "duration": "PT57M48.544S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6153900853001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6153900853001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6153900853001&_="+new Date().getTime() }; 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div><div container-id="cq-colctrl-lt0" comp-id="B5"><div class="text parbase section"><h3>Literature</h3><ul class="the-icons clearfix"><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/flow-cytometry-panel-builder-flyer.pdf" target="_blank">Flyer: Flow Cytometry Panel Design</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/novafluor-dyes-high-dimensional-design-flyer.pdf" target="_blank">Flyer: NovaFluor dyes for high-dimensional panel design</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/brilliant-ultra-violet-polymer-dyes-ebioscience-antibodies-flyer.pdf" target="_blank">Flyer: Brilliant Ultra Violet and Brilliant Violet polymer dyes conjugated to eBioscience antibodies</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/dna-based-dye-nanostructures-enable-directions-spectral-flow-cytometry-poster.pdf" target="_blank">Poster: DNA-Based Dye Nanostructures Enable New Directions in Spectral Flow Cytometry</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/leveraging-dna-fluorescent-technology-plex-flow-cytometry-poster.pdf" target="_blank">Poster: Leveraging DNA in fluorescent reporter technology to enable higher plex flow cytometry</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/designing-spectrally-clean-fluorescent-dyes-high-dimensional-biological-analysis-poster.pdf" target="_blank">Poster: Designing spectrally clean fluorescent dyes for high dimensional biological analysis</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><p><small class="muted">Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.<br> Brilliant Violet and PE CF dyes are subject to proprietary rights of Becton, Dickinson and Company.<br> Cy™ is a trademark of Amersham Biosciences Corp. Cy dyes are subject to proprietary rights of Amersham Biosciences Corp and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in vitro diagnostic use.<br> BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. 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