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class="content"><article class="post-484 post type-post status-publish format-standard has-post-thumbnail category-automation category-chromatography-data-system category-gc-ms tag-automation tag-chromatography-data-system tag-gc-ms tag-mosh-moah entry" aria-label="A Fully Automated MOSH/MOAH Workflow for Reliable Results More Easily"><header class="entry-header"><h1 class="entry-title">A Fully Automated MOSH/MOAH Workflow for Reliable Results More Easily</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/analyteguru/tf-author/petra-gerhards/" title="Posts by Petra Gerhards" rel="author">Petra Gerhards, </a> </span> <span class="post-info-date"> 07.25.2022 </span> </header><div class="entry-content"><div class="lia-message-body-content"> <p id="toc-hId-551948183"><em>Jorn Hofsteenge, SampleQ, The Netherlands, GC, GC-MS Application Specialist, contributed to this article.</em></p> <p>If the analysis of MOSH/MOAH (Mineral Oil Saturated Hydrocarbons and Mineral Oil Aromatic Hydrocarbons) is a challenge in your lab, you know how complicated the sample preparation can be.</p> <p data-unlink="true"><img decoding="async" class="alignnone wp-image-13086" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-MOSHMOAH-1-300x200.jpg" alt="" width="871" height="581" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-MOSHMOAH-1-300x200.jpg 300w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-MOSHMOAH-1-768x512.jpg 768w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-MOSHMOAH-1-96x65.jpg 96w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-MOSHMOAH-1.jpg 999w" sizes="(max-width: 871px) 100vw, 871px" />The EN 16995:2017describes: Foodstuff – Vegetable oils and foodstuff on basis of vegetable oils – Determination of MOSH/MOAH with on-line HPLC-GC-FID analysis (from C10 – C50). This European standard was approved by CEN (The European Committee for Standardisation) on March 10, 2017.[1]</p> <p>“The method has been tested in an interlaboratory study via the analysis of both naturally contaminated and spiked vegetable oil samples and mayonnaise and margarine samples, ranging from 4mg/kg to 196 mg/kg for MOSH, and from 2 mg/kg for MOAH. According to the results in the interlaboratory studies, the method has been proven suitable for MOSH and MOAH mass concentrations each above 10 mg/kg. In the case of suspected interferences from natural sources, the fossil origin of the MOSH and MOAH fraction can be verified by examination of the pattern by GC-MS.” [1]</p> <h3 id="toc-hId-1154349646"><strong>Definitions</strong></h3> <p>The following hydrocarbons can be analytically determined within the scope of this test specification:</p> <ul> <li>Mineral oil saturated hydrocarbons (MOSH): saturated hydrocarbons from mineral oil. These consist of kerosenes (open-chain hydrocarbons) and naphthenes (cyclic hydrocarbons), most of which are highly alkylated and either come directly from petroleum or are formed by the hydrogenation of aromatics and other conversion processes during refining. Much MOSH/MOAH also comes from contact with contaminated packaging material, exhaust fumes or even chemicals used in the extraction process.</li> </ul> <h3 id="toc-hId-2041853327"><strong>How do mineral oils get into food?</strong></h3> <p>Foodstuffs, however, can also contain residues of mineral oils even before packaging. On one hand, contamination can occur during the transport of foodstuffs in jute sacks contaminated with mineral oils. On the other hand, there is the possibility of mineral oil contamination during food production, e.g., through oily machine parts or through lubricating greases used during maintenance or cleaning. Mineral oil-containing wax coatings applied directly to foodstuffs or mineral oil-containing food additives used for surface treatment can also cause mineral oil contamination. Jute bags contaminated with mineral oil are still widely used in many countries.</p> <p>Furthermore, an environmental “basic contamination” of food raw materials with mineral oil hydrocarbons is possible, e.g., through exhaust gas from gasoline engines, emissions from energy supply and industrial plants as well as fine dust from asphalted roads. [3] There also can be intentional contamination (food fraud), as with Ukrainian sunflower oil in the 1990s.</p> <h3 id="toc-hId--1365610288"><strong>Actual situation</strong></h3> <p>MOSH/MOAH is an issue that has existed for many years.</p> <p><a href="https://www.foodwatch.org/fileadmin/-INT/mineral_oil/documents/2021-12-03_technical__minoil__project_report.pdf" target="_blank" rel="noopener nofollow noreferrer">Food Watch International</a> continuously monitors the MOSH/MOAH situation. There are many products that do not meet the criteria in the EU, which specifies a detection limit of 0.5 mg/kg.</p> <p>Recently, <a href="https://www.oekotest.de/essen-trinken/Olivenoel-Test-Bis-auf-eins-alle-mit-Mineraloel-verunreinigt_12637_1.html" target="_blank" rel="noopener nofollow noreferrer">German magazine</a> <em>Ökotest</em> analyzed 20 olive oils, and 19 of them were contaminated with MOSH and/or MOAH.</p> <p><a href="https://www.foodnavigator.com/Article/2021/12/16/MOSHs-or-MOAHs-in-food-Calls-for-zero-tolerance-as-toxic-mineral-oils-found-in-products-across-Europe-but-are-they-harming-consumers" target="_blank" rel="noopener nofollow noreferrer">Foodnavigator</a> also showed results for contaminated stock cubes.</p> <p>The <a href="https://www.foodpackagingforum.org/news/campaign-detects-mineral-oil-hydrocarbons-in-european-food-products" target="_blank" rel="noopener nofollow noreferrer">Foodpackagingforum</a> found products containing MOAH include bouillon powders, margarine, hazelnut spreads, cheese, cereal and chocolates. It also measured the presence of mineral oil saturated hydrocarbons (MOSH) in 92% of tested products, with concentrations ranging from 0.5 to 140 mg/kg.</p> <p>The list is endless and MOSH/MOAH becomes more and more of a problem in different matrices, further increasing the demand for a fully automated method.</p> <h3 id="toc-hId--478106607"><strong>What are the challenges?</strong></h3> <p>If you need to deliver a certificate of analysis before you can sell your product to the food industry, you already know the issues. However, there are a couple of challenges with determining MOSH/MOAH.</p> <ul> <li><strong>The k</strong>ey to success is using a heated line and heated valves, which helps avoid time-consuming cleaning procedures and the chance of unreliable results.</li> <li>Some people are still performing <strong>manual sample preparation</strong>. There are a lot of steps involved — and all can be prone to human error. Are you familiar with this situation in your lab? Look at our fully automated solution with the <a href="https://www.thermofisher.com/order/catalog/product/1R77010-2001" target="_blank" rel="nofollow noopener">Thermo Scientific™ TriPlus™ RSH™ SMART</a> autosampler.</li> <li>Are you still using up to <strong>four different software platforms</strong> within your complete process? If so, upgrade your MOSH/MOAH analysis to the next step. Learn how to automatically prep samples and report out using one solution — <a href="https://www.thermofisher.com/order/catalog/product/CHROMELEON7" target="_blank" rel="nofollow noopener">Thermo Scientific™Chromeleon™ Chromatography Data System (CDS)</a>software.</li> </ul> <p><span class="lia-inline-image-display-wrapper lia-image-align-center" style="width: 756px"><span class="lia-inline-image-caption"><img decoding="async" class="wp-image-13087 size-full aligncenter" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-1-1.png" alt="" width="756" height="404" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-1-1.png 756w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-1-1-300x160.png 300w" sizes="(max-width: 756px) 100vw, 756px" />Figure 1: FID chromatogram of a jute bag.</span></span></p> <p>Does this chromatogram look familiar to you? Are you still using Microsoft® Excel® for data treatment, leaving your software foundation and losing track of your data? We have a solution that is fully supported in Chromeleon CDS, and it doesn’t take hours to copy and paste data with our plugin solution. Minimize your errors, time and labor following our automated workflow.</p> <p><span class="lia-inline-image-display-wrapper lia-image-align-center" style="width: 756px"><span class="lia-inline-image-caption"><img loading="lazy" decoding="async" class="wp-image-13083 size-full aligncenter" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-2-1.png" alt="" width="756" height="409" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-2-1.png 756w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-2-1-300x162.png 300w" sizes="(max-width: 756px) 100vw, 756px" />Figure 2: Dual channel FID chromatogram with MOAH in the 1st channel and MOSH in the 2nd channel.</span></span></p> <p><span class="lia-inline-image-display-wrapper lia-image-align-center" style="width: 756px"><span class="lia-inline-image-caption"><img loading="lazy" decoding="async" class="wp-image-13084 size-full aligncenter" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-3-1.png" alt="" width="756" height="277" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-3-1.png 756w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-3-1-300x110.png 300w" sizes="(max-width: 756px) 100vw, 756px" />Figure 3: Fully automated report in Chromeleon CDS.</span></span></p> <h3><strong>And now the question is, how do we get there?</strong></h3> <p>The new in combination with the new and the <a href="https://www.thermofisher.com/order/catalog/product/1R77010-2001" target="_blank" rel="nofollow noopener">Thermo Scientific™ TriPlus™ RSH SMART</a>, is your combination of choice to generate MOSH/MOAH data according to the EN 16995:2017 method.</p> <p>It can be that easy. You simply use <a href="https://www.thermofisher.com/de/de/home/industrial/chromatography/chromatography-data-systems-cds.html?cid=E.22CMD.DL102.05714.01&https://www.thermofisher.com/us/en/home/industrial/chromatography/chromatography-data-systems-cds.html?cid=E.22CMD.DL102.05714.01&ef_id=EAIaIQobChMItfqSuuOV9gIVE9d3Ch2XOQswEAAYASAAEgLBkfD_BwE:G:s&s_kwcid=AL!3652!3!580298157087!p!!g!!cds%20software&gclid=EAIaIQobChMItfqSuuOV9gIVE9d3Ch2XOQswEAAYASAAEgLBkfD_BwE" target="_blank" rel="nofollow noopener">Chromeleon CDS</a>, stay in the same software platform and within 10 seconds the quantification is performed, and the results are ready to export. They can be exported into the local LIMS system for data reporting and/or into Microsoft Excel for control. The process is fully automated, and the results are the same in either Microsoft Excel or Chromeleon CDS. This is a proven combination that is already installed in labs across the world.</p> <p><span class="lia-inline-image-display-wrapper lia-image-align-center" style="width: 999px"><span class="lia-inline-image-caption"><img loading="lazy" decoding="async" class="wp-image-13085 size-full aligncenter" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-4-1.jpg" alt="" width="999" height="749" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-4-1.jpg 999w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-4-1-300x225.jpg 300w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Figure-4-1-768x576.jpg 768w" sizes="(max-width: 999px) 100vw, 999px" />Figure 4 : Photo of the integrated system in the lab.</span></span></p> <h3 id="toc-hId-1296900755"><strong>Would you like to see how </strong><strong>all</strong><strong> the technology works together?</strong></h3> <p data-unlink="true"><strong>View this video </strong>from our partner, SampleQ, below. Or visit <a href="https://www.sampleq.com/food" target="_self" rel="nofollow noopener noreferrer">SampleQ</a></p> <p><a href="https://players.brightcove.net/3663210762001/default_default/index.html?videoId=6309380550112" target="_blank" rel="noopener" data-wplink-edit="true"><span class="lia-inline-image-display-wrapper lia-image-align-center" style="width: 756px"><img loading="lazy" decoding="async" class="aligncenter wp-image-13100 size-full" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Video-Image-1.png" alt="" width="756" height="382" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Video-Image-1.png 756w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/072522-Video-Image-1-300x152.png 300w" sizes="(max-width: 756px) 100vw, 756px" /></span></a></p> <p><strong>To see the recent webinar</strong>, Analysis of mineral oil saturated hydrocarbons and mineral oil aromatic hydrocarbons (MOSH/MOAH), <a id="link_6" class="page-link lia-link-navigation lia-custom-event" href="https://www.analyteguru.com/t5/webinar-library/analysis-of-mineral-oil-saturated-hydrocarbons-and-mineral-oil/ta-p/18025?ce=E.22CMD.EL104.09325.01&cid=E.22CMD.EL104.09325.01" target="_blank" rel="noopener">click here.</a></p> <h3 id="toc-hId--2110562860"><strong>Conclusions</strong></h3> <p>Use an easy and proven plug-and-play method for the determination of MOSH/MOAH. This headache-free sample preparation is fully automated with the Tri Plus RSH SMART technology. With the newest standard in HPLC technology, the <a href="https://www.thermofisher.com/us/en/home/products-and-services/promotions/industrial/vanquish-hplc-uhplc-systems.html" target="_blank" rel="nofollow noopener">Thermo Scientific™ Vanquish™</a> normal phase system — in combination with the TRCE 1600 GC series — you are equipped with state-of-the-art instrument technology. And the nicest part is thatthis solution is all controlled from one software platform: <a href="https://www.thermofisher.com/order/catalog/product/CHROMELEON7" target="_blank" rel="nofollow noopener">Thermo Scientific™ Chromeleon™ Chromatography Data System</a> (CDS).You place your sample in the autosampler, press start and get a complete analysis for MOSH/MOAH according to the EN 16995:2017 method.</p> <p>With the right products, tools, and technology, you can get reliable results easily, saving time and reducing errors in your lab.</p> <p>To learn more about our automated solutions in cooperation with SampleQ, visit:</p> <p><a href="http://www.thermofisher.com/gc-automation" target="_blank" rel="nofollow noopener">www.thermofisher.com/gc-automation</a>.</p> <h3 id="toc-hId--1223059179"><strong>Resources</strong></h3> <p>[1] EN 16995:2917 (E)</p> <p>[2] Bestimmung von Kohlenwasserstoffen aus Mineralöl (MOSH und MOAH) oder Kunststoffen (POSH, PAO) in Verpackungsmaterialien und trockenen Lebensmitteln mittels Festphasenextraktion und GC-FID) – Analysenmethode (BfR)</p> <p>[3] <a href="https://www.lci-koeln.de/deutsch/veroeffentlichungen/lci-focus/mineraloelrueckstaende-in-lebensmitteln-mosh-und-moah" target="_blank" rel="noopener nofollow noreferrer">https://www.lci-koeln.de/deutsch/veroeffentlichungen/lci-focus/mineraloelrueckstaende-in-lebensmitte…</a></p> <h3 id="toc-hId-551948183"></h3> </div> </div></article><section class="author-box"><h4 class="author-box-title">Petra Gerhards</h4><div class="author-box-content" itemprop="description">Petra Gerhards, Dipl-Ing, is Regional Marketing Manager of GC and GC-MS for EMEA at Thermo Fisher Scientific. She has more than 29 years of experience in the fields of GC-MS, SPE and LC-MS. Since joining the regional team she has contributed to workflow solutions combining vials and closures with SPE solutions, GC-MS and LC-MS. She works with KOL's on data for regional specific marketing campaigns, organizes in-house seminars and works on customer specific solutions. Her main expertise is in the field of doping and drugs-of-abuse analysis.</div></section><div class="adjacent-entry-pagination pagination"><div class="pagination-previous alignleft"><i class="fa fa-icon fa-chevron-left"></i> <a href="https://www.thermofisher.com/blog/analyteguru/feeling-sample-prep-burnout-let-your-gc-autosampler-help-with/" rel="prev">Feeling Sample Prep Burnout? Let Your GC Autosampler Help With Your Workload</a></div><div class="pagination-next alignright"><a href="https://www.thermofisher.com/blog/analyteguru/determining-sorbitol-in-complex-food-samples-a-reliable-and/" rel="next">Determining Sorbitol in Complex Food Samples: A Reliable, Convenient HPAE-PAD Method</a> <i class="fa fa-icon fa-chevron-right"></i></div></div></main></div></div><footer class="site-footer"><div class="wrap"><div class="creds"><p class="footer-links"><a href="https://corporate.thermofisher.com/en/footer/privacy-statement.html" target="_blank" rel="noopener noreferrer">Privacy Statement</a><a href="https://corporate.thermofisher.com/en/footer/terms-and-conditions.html" target="_blank" rel="noopener noreferrer">Terms & Conditions</a><a href="/blog/locations/">Locations</a><a href="/blog/sitemap/">Sitemap</a></p><p>© 2024 <a href="https://www.thermofisher.com">Thermo Fisher Scientific</a>. 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