TESSA: Scalable AAV Production

<!DOCTYPE html> <html lang="en"> <head><title> TESSA: Scalable AAV Production - OXGENE </title><meta property="og:title" content="TESSA: Scalable AAV Production" /><meta property="og:site:name" content="OXGENE" /><meta property="og:url" content="https://oxgene.com/gene_therapy/tessa_scalable_aav_production.aspx" /><meta property="og:type" content="article" /><meta property="article:published:time" content="2021-08-16T11:19Z" /><meta property="article:modified:time" content="2024-07-01T20:08Z" /><meta property="og:locale" content="en-GB" /><meta property="twitter:title" content="TESSA: Scalable AAV Production" /><meta property="twitter:url" content="https://oxgene.com/gene_therapy/tessa_scalable_aav_production.aspx" /><meta property="twitter:card" content="summary" /><link rel="canonical" href="https://oxgene.com/gene_therapy/tessa_scalable_aav_production.aspx" /><meta id="responsive_meta" name="viewport" content="user-scalable=no, initial-scale=1, maximum-scale=1.0, 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li_nav_careers"><a class=" nav_careers" href='https://careers-oxgene.icims.com/jobs/intro'>Careers <b class="glyphicon glyphicon-chevron-right visible-xs-inline"></b></a></li><li class=" li_nav_contact"><a class=" nav_contact" href='/about/contact.aspx'>Contact <b class="glyphicon glyphicon-chevron-right visible-xs-inline"></b></a></li></ul></li><li class=" li_nav_search"><span class="search_btn"></span></li></ul> </div> </div> </nav> </div> </header> <section id="page"> <article id="content"> <div class="container"> </div> <div class="hd_container container intro_strapline">  <h2 class="alternative"><a href="/gene_therapy/default.aspx">GENE THERAPY SOLUTIONS</a></h2>  </div> <div class="container intro_head"> <ul class='side_menu hidden-xs '><li class=" li_nav_plasmids_and_cell_lines"><a class=" nav_plasmids_and_cell_lines" href='/gene_therapy/plasmids_and_cell_lines.aspx'>Plasmids and Cell Lines</a></li><li class=" li_nav_lv_packaging_producer_cell_lines"><a class=" nav_lv_packaging_producer_cell_lines" href='/gene_therapy/transient_aav_production.aspx'>Transient AAV Production</a></li><li class=" li_nav_adenoviral_production_systems"><a class=" nav_adenoviral_production_systems" href='/gene_therapy/tessa_scalable_aav_production.aspx'>TESSA: Scalable AAV Production</a></li></ul>  <h1>TESSA: SCALABLE AAV<br /> <strong>PRODUCTION</strong></h1> <p class="lg_p">Our proprietary self-silencing adenoviral vectors deliver high titre, high quality AAV to gene therapy pioneers. We engineer <strong>TESSA™</strong> vectors to encode your gene or capsid of interest for high quality manufacture of AAV seed stocks. before process development and rAAV manufacture at any grade or scale.</p>  </div> <div class="container-fluid">  <section class="img_divider_widget"> <div class="img_divider_image"> <div class="container"> <p><img src="/_userfiles/pages/images/gene_therapy/lentiviral_plasmids.jpg" style="width: 90%; height: 90%;" /></p> </div> </div> <div class="img_divider_content"> <div class="container"> <h2 class="alternative">"Overall, TESSA™ enables us to test new constructs much faster and cheaper now which is why we like to keep using it."</h2> <h3 style="text-align: right;">Dr Harald Hartweger, The Rockefeller University</h3> </div> </div> </section> <section class="paragraph_widget"> <div class="container"> <h3>Tetracycline enabled self silencing adenovirus (TESSA™) technology</h3> <p class="lg_p">TESSA™ vectors deliver a step-change in AAV manufacture; an easily scalable, contaminant-, chemical- and transfection free technology.</p> <p class="lg_p">TESSA™ vectors take advantage of the adenoviral lifecycle, which contains two temporal phases; early and late. The early phase provides all the adenoviral help needed for high fidelity, high titre AAV manufacture. The late phase leads to adenovirus contamination. We’ve engineered supremely tight regulation of the adenoviral late region, which is wholly responsible for producing adenovirus structural proteins. Using TESSA™ technology, full early region expression, which provides all the help needed for AAV production, is still enabled, but the late region gene expression is turned off, meaning there is no adenoviral structural protein production. This represses adenovirus production by 99.99999 -100% during an AAV manufacturing run, while increasing rAAV yield, packaging efficiency and infectivity compared to both Ad5 helper plasmid or wild-type adenovirus.</p> <p class="lg_p">Stable integration of AAV Rep and AAV Cap into the TESSA™ vector allows efficient and simultaneous delivery of all the necessary components for AAV replication in a single agent.</p> </div> </section> <section class="divider_widget blue"> <div class="container"> <div class="left_col"> <h2 class="alternative">Evaluate TESSA™ technology</h2> </div> <div class="right_col"> <p class="lg_p" style="text-align: right;"><a class="orange_btn" href="/forms/order_tessa.aspx">ORDER NOW</a></p> </div> </div> </section> <p> </p> <section class="quote_widget"> <div class="container"> <div class="quote_block"> <p>All of this [TESSA + plasmid vs TESSA + AAV] was way better than helper free triple transfection in the same experiment, which gave us 26-180 times less yield depending on what you compare.</p> </div> </div> </section> <p> </p> <section class="paragraph_widget"> <div class="container"> <h3>How it works</h3> <p class="lg_p">There are two stages to AAV manufacture with TESSA™. The first is to produce initial AAV seed stock. This can be done by plasmid transfection, for example with our transient AAV system, with a second TESSA™ vector encoding the AAV genome and gene of interest (a process we call TESSA 2.0 and which gives more efficient delivery than transfection), or any alternative existing approach.</p> <p class="lg_p">Overall, producing AAV2 using two TESSA™ vectors gives approximately a 40-fold increase<br /> in AAV particle yields compared to the helper free system. We also observe a >2400 fold cumulative increase in AAV2 infectious yield (1 in 6 genome-containing AAV2 particles is infectious from TESSA, compared to 1 in 1200 from plasmid based transfection; see data below), and an increased percentage of full capsids from 2-5% up to 70%.</p> <p class="lg_p">In the second stage, called TESSA Pro, this AAV seed stock and another TESSA-AAV-Rep-Cap vector can be used to co-infect HEK293 cells. This leads to further AAV replication, so increasing the final AAV yield. Interestingly, whether you use AAV seed stock generated using two TESSA vectors or three plasmid transfection, the final AAV yield is comparable (see data below).</p> <h3>Advantages</h3> <p class="lg_p">TESSA™ technology is fully scalable and significantly reduces the number of input materials required for AAV manufacture. It’s ideally suited for rapid and continuous production of GMP quality AAV, reducing the potential for batch to batch variation. Ultimately, TESSA™ technology will result in improved cost of goods, and improved safety of gene therapies.</p> </div> </section> <div class="editable_expandable spacer"> <div aria-expanded="false" class="expandable_heading collapsed" data-target="#panel118" data-toggle="collapse"> <h4 class="expandable_title">Adenoviral structural genes are regulated by negative feedback loop</h4> </div> <div aria-expanded="false" class="expandable_collapse collapse" id="panel118" style="height: 0px;"> <div class="expandable_body"> <p><img alt="" src="/_userfiles/pages/images/gene_therapy/tessafeedbackloop_1080x517.png" style="width: 100%;" /></p> <p>A Tet repressor (TetR) binding site is inserted into the Major late promoter, while the TetR gene itself is encoded within the late region. This creates a negative feedback loop, where adenoviral structural proteins can only be produced in the presence of doxycycline.</p> </div> </div> <div class="clearer"> </div> </div> <div class="editable_expandable spacer"> <div aria-expanded="false" class="expandable_heading collapsed" data-target="#panel512" data-toggle="collapse"> <h4 class="expandable_title">Using two TESSA™ vectors improves rAAV yield and infectivity</h4> </div> <div aria-expanded="false" class="expandable_collapse collapse" id="panel512" style="height: 0px;"> <div class="expandable_body"> <p><img alt="" src="/_userfiles/pages/images/gene_therapy/tessaproducesmore_1080x532.png" style="width: 100%;" /></p> <p>Left: using two TESSA vectors (blue) to produce rAAV results in a 42-fold improvement in rAAV2 yield compared to the helper free system (grey). Right: rAAV2 produced using two TESSA vectors (blue) is also significantly more infectious than rAAV2 produced using the helper free system (grey) by TICD50. In fact, 1 in 6 rAAV2 particles containing a genome made from TESSA vectors is infectious compared to 1 in 1200 from plasmids (grey).</p> </div> </div> <div class="clearer"> </div> </div> <div class="editable_expandable spacer"> <div aria-expanded="false" class="expandable_heading collapsed" data-target="#panel765" data-toggle="collapse"> <h4 class="expandable_title">Adding TESSA™ to rAAV seed stock continues rAAV propagation</h4> </div> <div aria-expanded="false" class="expandable_collapse collapse" id="panel765" style="height: 0px;"> <div class="expandable_body"> <p><img alt="" src="/_userfiles/pages/images/gene_therapy/tessaaavpropagation_1080x707.png" style="width: 100%;" /></p> <p>Two viruses in, one virus out: The initial AAV seed stock can be produced from either a normal 3-plasmid transient system, a TESSA™ vector plus gene of interest (GOI) plasmid, or two TESSA™ vectors (right). Once the initial AAV seed stock has been produced, this can be harvested and used alongside more TESSA™ vector to co-infect cells and produce more of the AAV. This step could be repeated as part of a continuous manufacturing process.</p> </div> </div> <div class="clearer"> </div> </div> <div class="editable_expandable spacer"> <div aria-expanded="false" class="expandable_heading collapsed" data-target="#panel671" data-toggle="collapse"> <h4 class="expandable_title">Source of rAAV seed stock does not affect final yield</h4> </div> <div aria-expanded="false" class="expandable_collapse collapse" id="panel671" style="height: 0px;"> <div class="expandable_body"> <p><img alt="" src="/_userfiles/pages/images/gene_therapy/sourceofaavstartingmaterial_1080x532.png" style="width: 100%;" /></p> <p>Left: Despite giving a higher initial yield (column 3, blue) than the helper free system (column 5, grey), when that rAAV seed stock is used to co-infect HEK293 cells with a second TESSA vector, the resulting rAAV2 yields are comparable (columns 1 and 2, blue). Right: However, while still higher than with helper free production (grey), infectious AAV2 yields are slightly lower after a second round of rAAV2 production (columns 1 and 2, vs column 3).</p> </div> </div> <div class="clearer"> </div> </div> <p> </p> <section class="divider_widget grey"> <div class="container"> <div class="left_col"> <h2 class="alternative">Are you ready for GMP Manufacture?</h2> <p>WuXi Advanced Therapies is a global Contract Testing, Development<br /> and Manufacturing Organization (CTDMO) dedicated to accelerating<br /> progress and time to market for cell and gene therapies.</p> </div> <div class="right_col"> <p style="text-align: right;"><a class="orange_btn" href="/gmp_manufacture_testing.aspx">GMP MANUFACTURE</a></p> </div> </div> </section>  </div> </article> </section> <footer id="footer"> <div class="hd_container container"> <div class="row"> <div class="col-xs-12"> <div class="footer_logo"> <svg xmlns="http://www.w3.org/2000/svg" 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document.all["ctl25_RFVFirstName"] : document.getElementById("ctl25_RFVFirstName"); ctl25_RFVFirstName.controltovalidate = "ctl25_TBFirstName"; ctl25_RFVFirstName.errormessage = "Please enter your first name"; ctl25_RFVFirstName.display = "Dynamic"; ctl25_RFVFirstName.validationGroup = "leads"; ctl25_RFVFirstName.evaluationfunction = "RequiredFieldValidatorEvaluateIsValid"; ctl25_RFVFirstName.initialvalue = ""; var ctl25_RFVLastName = document.all ? document.all["ctl25_RFVLastName"] : document.getElementById("ctl25_RFVLastName"); ctl25_RFVLastName.controltovalidate = "ctl25_TBLastName"; ctl25_RFVLastName.errormessage = "Please enter your last name"; ctl25_RFVLastName.display = "Dynamic"; ctl25_RFVLastName.validationGroup = "leads"; ctl25_RFVLastName.evaluationfunction = "RequiredFieldValidatorEvaluateIsValid"; ctl25_RFVLastName.initialvalue = ""; var ctl25_RFVCompany = document.all ? document.all["ctl25_RFVCompany"] : document.getElementById("ctl25_RFVCompany"); ctl25_RFVCompany.controltovalidate = "ctl25_TBCompany"; ctl25_RFVCompany.errormessage = "Please enter your company name"; ctl25_RFVCompany.display = "Dynamic"; ctl25_RFVCompany.validationGroup = "leads"; ctl25_RFVCompany.evaluationfunction = "RequiredFieldValidatorEvaluateIsValid"; ctl25_RFVCompany.initialvalue = ""; var ctl25_RFVEmail = document.all ? document.all["ctl25_RFVEmail"] : document.getElementById("ctl25_RFVEmail"); ctl25_RFVEmail.controltovalidate = "ctl25_TBEmail"; ctl25_RFVEmail.errormessage = "Please enter your email address"; ctl25_RFVEmail.display = "Dynamic"; ctl25_RFVEmail.validationGroup = "leads"; ctl25_RFVEmail.evaluationfunction = "RequiredFieldValidatorEvaluateIsValid"; ctl25_RFVEmail.initialvalue = ""; var ctl25_REVEmail = document.all ? document.all["ctl25_REVEmail"] : document.getElementById("ctl25_REVEmail"); ctl25_REVEmail.controltovalidate = "ctl25_TBEmail"; ctl25_REVEmail.errormessage = "<span class=\'help-block\'>Invalid email address</span>"; ctl25_REVEmail.display = "Dynamic"; ctl25_REVEmail.validationGroup = "leads"; ctl25_REVEmail.evaluationfunction = "RegularExpressionValidatorEvaluateIsValid"; ctl25_REVEmail.validationexpression = "^\\w+([-+.\']\\w+)*@\\w+([-.]\\w+)*\\.\\w+([-.]\\w+)*$"; var ctl25_RFVLocation = document.all ? document.all["ctl25_RFVLocation"] : document.getElementById("ctl25_RFVLocation"); ctl25_RFVLocation.controltovalidate = "ctl25_DDLLocation"; ctl25_RFVLocation.errormessage = "Please select your location"; ctl25_RFVLocation.display = "Dynamic"; ctl25_RFVLocation.validationGroup = "leads"; ctl25_RFVLocation.evaluationfunction = "RequiredFieldValidatorEvaluateIsValid"; ctl25_RFVLocation.initialvalue = ""; var ctl25_ctl01_BotCheckBoxValidator = document.all ? document.all["ctl25_ctl01_BotCheckBoxValidator"] : document.getElementById("ctl25_ctl01_BotCheckBoxValidator"); ctl25_ctl01_BotCheckBoxValidator.errormessage = "Please uncheck this box to continue"; ctl25_ctl01_BotCheckBoxValidator.validationGroup = "leads"; ctl25_ctl01_BotCheckBoxValidator.evaluationfunction = "CustomValidatorEvaluateIsValid"; ctl25_ctl01_BotCheckBoxValidator.clientvalidationfunction = "validateCheckboxctl25_ctl01_CBBot"; var ctl25_ctl01_BotValidator = document.all ? document.all["ctl25_ctl01_BotValidator"] : document.getElementById("ctl25_ctl01_BotValidator"); ctl25_ctl01_BotValidator.validationGroup = "leads"; ctl25_ctl01_BotValidator.evaluationfunction = "CustomValidatorEvaluateIsValid"; //]]> </script> <script type="text/javascript"> //<![CDATA[ var Page_ValidationActive = false; if (typeof(ValidatorOnLoad) == "function") { ValidatorOnLoad(); } function ValidatorOnSubmit() { if (Page_ValidationActive) { return ValidatorCommonOnSubmit(); } else { return true; } } //]]> </script> </form> <script src="/_resources/js/master/master.min.v20220920171055.js" type="text/javascript"></script> <script src="/_resources/js/include_masterpage.min.v20210903123328.js" type="text/javascript"></script>  <div id="blueimp-gallery" class="blueimp-gallery">  <div class="slides"></div>  <h3 class="title"></h3> <a class="prev">‹</a> <a class="next">›</a> <a class="close">×</a> <a class="play-pause"></a> <ol class="indicator"></ol>  <div class="modal fade"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" aria-hidden="true">×</button> <h4 class="modal-title"></h4> </div> <div class="modal-body next"></div> <div class="modal-footer"> <button type="button" class="btn btn-default pull-left prev"> <i class="glyphicon glyphicon-chevron-left"></i> Previous </button> <button type="button" class="btn btn-primary next"> Next <i class="glyphicon glyphicon-chevron-right"></i> </button> </div> </div> </div> </div> </div> </body> </html>

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