Tracking protein-protein interactions by NMR: conformational selection in human steroidogenic cytochrome P450 CYP17A1 induced by cytochrome b5

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new collection</label> </li> <li> <input type="radio" id="collections-action-panel-existing" class="collections-existing" name="collections" value="existing" checked="true" data-ga-category="save_share" data-ga-action="collections" data-ga-label="collections_radio_existing"> <label for="collections-action-panel-existing">Add to an existing collection</label> </li> </ul> </div> <div class="controls-wrapper"> <div class="action-panel-control-wrap new-collections-controls"> <label for="collections-action-panel-add-to-new" class="action-panel-label required-field-asterisk"> Name your collection: </label> <input type="text" name="add-to-new-collection" id="collections-action-panel-add-to-new" class="collections-action-add-to-new" pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/" maxlength="100" data-ga-category="save_share" data-ga-action="create_collection" data-ga-label="non_favorties_collection"> <div class="collections-new-name-too-long usa-input-error-message selection-validation-message"> Name must be less than 100 characters </div> </div> <div class="action-panel-control-wrap existing-collections-controls"> <label for="collections-action-panel-add-to-existing" class="action-panel-label"> Choose a collection: </label> <select id="collections-action-panel-add-to-existing" class="action-panel-selector collections-action-add-to-existing" data-ga-category="save_share" data-ga-action="select_collection" data-ga-label="($('#collections-action-add-to-existing').val() === 'Favorites') ? 'Favorites' : 'non_favorites_collection'"> </select> <div class="collections-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your collection due to an error<br> <a href="#">Please try again</a> </div> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore data-ga-category="save_share" data-ga-action="collections" data-ga-label="add"> Add </button> <button class="action-panel-cancel" aria-label="Close 'Add to Collections' panel" ref="linksrc=close_collections_panel" aria-controls="collections-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="collections" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="bibliography-action-panel" class="bibliography-action-panel action-panel in-progress-dots-panel" aria-hidden="true" data-bibliography-open-panel-enabled="false" data-bibliography-open-panel-url-hash="#open-bibliography-panel"> <div class="inner-wrap"> <h3 class="action-panel-heading"> Add to My Bibliography </h3> <form id="bibliography-action-panel-form" class="bibliography-action-panel-form action-panel-content action-form action-panel-smaller-selectors" data-add-to-bibliography-max-amount="100" data-add-to-bibliography-batch-size="10" data-bibliography-delegates-url="/list-bibliography-delegates/" data-add-to-bibliography-url="/add-to-bibliography/" data-get-article-ids-by-search-url="/get-article-ids-by-search/" data-mybib-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/mybibliography/"> <input type="hidden" name="csrfmiddlewaretoken" value="eIuCQYwz1dqHsdoBTdf8gs2n5VMuNMwNglN76LYtPYmjXIWYj2qd4wgmS7dsPJQ7"> <div class="action-panel-control-wrap bibliographies-controls"> <div class="choice-group"> <ul class="bibliographies-action-add radio-group-items"> <li> <input name="bibliography" id="my-bibliography" class="my-bibliography" type="radio" checked/> <label for="my-bibliography">My Bibliography</label> </li> </ul> </div> </div> <div class="bibliographies-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your delegates due to an error<br> <a href="#">Please try again</a> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore> Add </button> <button class="action-panel-cancel" aria-label="Close 'Add to bibliography' panel" ref="linksrc=close_bibliography_panel" aria-controls="bibliography-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="mybib" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="saved-search-action-panel" class="saved-search-action-panel action-panel " aria-hidden="true" data-saved-search-open-panel-enabled="false" data-saved-search-open-panel-url-hash="#open-saved-search-panel"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Your saved search </h2> <form id="saved-search-action-panel-form" class="saved-search-action-panel-form action-panel-content action-form" data-create-saved-search-url="/create-saved-search/" data-try-search-terms-url="/try-search-term/" data-saved-search-root-url="https://www.ncbi.nlm.nih.gov/myncbi/searches/"> <input type="hidden" name="csrfmiddlewaretoken" value="eIuCQYwz1dqHsdoBTdf8gs2n5VMuNMwNglN76LYtPYmjXIWYj2qd4wgmS7dsPJQ7"> <div class="action-panel-control-wrap"> <label for="saved-search-name" class="action-panel-label saved-search-name-label required-field-asterisk"> Name of saved search: </label> <input maxlength="200" type="text" name="saved-search-name" id="saved-search-name" class="saved-search-name" value="" required pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/"> </div> <div class="action-panel-control-wrap"> <label for="saved-search-term" class="action-panel-label required-field-asterisk"> Search terms: </label> <textarea name="saved-search-term" id="saved-search-term" class="saved-search-term" required></textarea> </div> <div class="test-search-term-wrap"> <a href="#" class="try-search-term">Test search terms</a> </div> <div class="choice-group action-panel-extra-margin-top"> <span class="action-panel-label" id="fieldset-label"> Would you like email updates of new search results? </span> <fieldset id="saved-search-alert" aria-describedby="fieldset-label"> <legend class="usa-sr-only">Saved Search Alert Radio Buttons</legend> <ul class="radio-group-items"> <li> <input type="radio" id="saved-search-alert-yes" class="saved-search-alert-yes" name="saved-search-alert" value="yes" checked> <label for="saved-search-alert-yes" class="action-panel-label">Yes</label> </li> <li> <input aria-label="No radio input" type="radio" id="saved-search-alert-no" class="saved-search-alert-no" name="saved-search-alert" value="no"> <label for="saved-search-alert-no" class="action-panel-label">No</label> </li> </ul> </fieldset> </div> <div class="alert-schedule-wrap"> <div class="action-panel-control-wrap"> <label class="action-panel-label"> Email: </label> <span aria-label="Email address" id="saved-search-email" class="action-panel-label"><span class="action-panel-label-bold"></span> (<a class="myncbi-account-settings" href="https://www.ncbi.nlm.nih.gov/account/settings/">change</a>)</span> </div> <div class="action-panel-control-wrap action-panel-extra-margin-top"> <label for="saved-search-frequency" class="action-panel-label"> Frequency: </label> <select id="saved-search-frequency" class="no-border-panel-selector saved-search-frequency"> <option value="monthly">Monthly</option> <option value="weekly">Weekly</option> <option value="daily">Daily</option> </select> </div> <div class="action-panel-control-wrap saved-search-monthly-additional"> <label for="saved-search-monthly-on-day" class="action-panel-label"> Which day? </label> <select id="saved-search-monthly-on-day" class="no-border-panel-selector"> <option value="Sunday">The first Sunday</option> <option value="Monday">The first Monday</option> <option value="Tuesday">The first Tuesday</option> <option value="Wednesday">The first Wednesday</option> <option value="Thursday">The first Thursday</option> <option value="Friday">The first Friday</option> <option value="Saturday">The first Saturday</option> <option value="day">The first day</option> <option value="weekday">The first weekday</option> </select> </div> <div class="action-panel-control-wrap saved-search-weekly-additional"> <label for="saved-search-weekly-on-day" class="action-panel-label"> Which day? </label> <select id="saved-search-weekly-on-day" class="no-border-panel-selector saved-search-weekly-on-day"> <option value="Sunday">Sunday</option> <option value="Monday">Monday</option> <option value="Tuesday">Tuesday</option> <option value="Wednesday">Wednesday</option> <option value="Thursday">Thursday</option> <option value="Friday">Friday</option> <option value="Saturday">Saturday</option> </select> </div> <div class="action-panel-control-wrap"> <label for="saved-search-report" class="action-panel-label"> Report format: </label> <select id="saved-search-report" class="no-border-panel-selector saved-search-report"> <option value="DocSum">Summary</option> <option value="DocSumText">Summary (text)</option> <option value="Abstract">Abstract</option> <option value="AbstractText">Abstract (text)</option> <option value="MEDLINE">PubMed</option> </select> </div> <div class="action-panel-control-wrap"> <label for="saved-search-amount" class="action-panel-label"> Send at most: </label> <select id="saved-search-amount" class="no-border-panel-selector saved-search-amount"> <option value="1">1 item</option> <option value="5" selected>5 items</option> <option value="10">10 items</option> <option value="20">20 items</option> <option value="50">50 items</option> <option value="100">100 items</option> <option value="200">200 items</option> </select> </div> <div> <input type="checkbox" id="saved-search-send-if-no-result" class="saved-search-send-if-no-result" name="saved-search-send-if-no-result"> <label for="saved-search-send-if-no-result" class="action-panel-label smaller-checkbox"> Send even when there aren't any new results </label> </div> <div class="action-panel-control-wrap option-text-in-email-wrap"> <label for="saved-search-email-text" class="action-panel-label"> Optional text in email: </label> <textarea name="saved-search-email-text" id="saved-search-email-text" class="saved-search-email-text"></textarea> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Saving..." data-ga-category="save_share" data-ga-action="alert" data-ga-label="save"> Save </button> <button class="action-panel-cancel" aria-label="Close 'Your saved search' panel" ref="linksrc=close_saved_search_panel" aria-controls="saved-search-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="alert" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="citation-manager-action-panel" class="citation-manager-action-panel action-panel" aria-hidden="true"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Create a file for external citation management software </h2> <form id="citation-manager-action-panel-form" class="action-panel-content action-form" action="/results-export-ids/" data-by-search-action="/results-export-search-data/" data-by-ids-action="/results-export-ids/" method="post" data-by-search-method="post" data-by-ids-method="post"> <input type="hidden" name="csrfmiddlewaretoken" value="eIuCQYwz1dqHsdoBTdf8gs2n5VMuNMwNglN76LYtPYmjXIWYj2qd4wgmS7dsPJQ7"> <input name="results-format" type="hidden" value="pubmed"/> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Sending..." data-ga-category="save_share" data-ga-action="citation_manager" data-ga-label="save"> Create file </button> <button class="action-panel-cancel" aria-label="Close 'Send citations to citation manager' panel" ref="linksrc=close_citation_manager_panel" aria-controls="citation-manager-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="citation_manager" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="rss-action-panel" class="rss-action-panel action-panel " aria-hidden="true"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Your RSS Feed </h2> <form id="rss-action-panel-form" class="rss-action-panel-form action-panel-content action-form" data-create-rss-feed-url="/create-rss-feed-url/" data-search-form-term-value=""> <input type="hidden" name="csrfmiddlewaretoken" value="eIuCQYwz1dqHsdoBTdf8gs2n5VMuNMwNglN76LYtPYmjXIWYj2qd4wgmS7dsPJQ7"> <div class="action-panel-control-wrap"> <label for="rss-name" class="action-panel-label required-field-asterisk"> Name of RSS Feed: </label> <input maxlength="200" placeholder="Name" type="text" name="rss-name" id="rss-name" class="rss-name" value='' required pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/"> </div> <div class="rss-limit-wrap"> <div class="action-panel-control-wrap action-panel-extra-margin-top"> <label for="rss-limit" class="action-panel-label"> Number of items displayed: </label> <select id="rss-limit" class="no-border-panel-selector rss-limit"> <option value="5">5</option> <option value="10">10</option> <option value="15" selected="selected">15</option> <option value="20">20</option> <option value="50">50</option> <option value="100">100</option> </select> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Creating..." data-ga-category="save_share" data-ga-action="alert" data-ga-label="save"> Create RSS </button> <button class="action-panel-cancel" aria-label="Close 'Your RSS' panel" ref="linksrc=close_rss_panel" aria-controls="rss-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="alert" data-ga-label="cancel"> Cancel </button> </div> <div class="action-panel-control-wrap rss-link-copy-wrap"> <label for="rss-link" class="usa-sr-only">RSS Link</label> <input placeholder="Your RSS Feed Link" type="text" name="rss-link" id="rss-link" class="rss-link" title="RSS Link"> <button type="button" disabled class="rss-link-copy-button disabled" data-ga-category="save_share" data-ga-action="rss" data-ga-label="copy"> Copy </button> </div> </form> </div> </div> </div> </div> <div class="article-page" id="article-page" data-article-pmid="38842434"> <aside class="page-sidebar"> <div class="inner-wrap"> <div class="full-text-links"> <div class="full-view"> <h3 class="title"> Full text links </h3> <div class="full-text-links-list"> <a class="link-item dialog-focus" href="https://doi.org/10.1039/d4cp01268b" target="_blank" rel="noopener" ref="linksrc=fulltextorjournal_fulltext&is_pmc=False&PrId=4828&itool=Abstract-def&log$=linkouticon&uid=38842434&db=pubmed&nlmid=100888160" title="See full text options at Royal Society of Chemistry" data-ga-category="full_text" data-ga-action="Royal Society of Chemistry" data-ga-label="38842434" ><img src="https://cdn.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.rsc-cdn.org-oxygen-assets-logo-rsc-logo-btn-small.gif" alt="Royal Society of Chemistry full text link"><span class="text"> Royal Society of Chemistry </span></a><a class="link-item pmc " href="https://pmc.ncbi.nlm.nih.gov/articles/pmid/38842434/" target="_blank" rel="noopener" ref="linksrc=fulltextorjournal_fulltext&is_pmc=True&PrId=3494&itool=Abstract-def&log$=linkouticon&uid=38842434&db=pubmed&nlmid=100888160" title="Free full text at PubMed Central" data-ga-category="full_text" data-ga-action="PMC" data-ga-label="38842434" ><span class="text"> Free 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name="csrfmiddlewaretoken" value="eIuCQYwz1dqHsdoBTdf8gs2n5VMuNMwNglN76LYtPYmjXIWYj2qd4wgmS7dsPJQ7"><div class="choice-group" role="radiogroup"><ul class="radio-group-items"><li><input type="radio" id="collections-action-dialog-new" class="collections-new" name="collections" value="new" data-ga-category="collections_button" data-ga-action="click" data-ga-label="collections_radio_new"><label for="collections-action-dialog-new">Create a new collection</label></li><li><input type="radio" id="collections-action-dialog-existing" class="collections-existing" name="collections" value="existing" checked="true" data-ga-category="collections_button" data-ga-action="click" data-ga-label="collections_radio_existing"><label for="collections-action-dialog-existing">Add to an existing collection</label></li></ul></div><div class="controls-wrapper"><div class="action-panel-control-wrap new-collections-controls"><label for="collections-action-dialog-add-to-new" class="action-panel-label required-field-asterisk"> Name your collection: </label><input type="text" name="add-to-new-collection" id="collections-action-dialog-add-to-new" class="collections-action-add-to-new" pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/" maxlength="100" data-ga-category="collections_button" data-ga-action="create_collection" data-ga-label="non_favorties_collection"><div class="collections-new-name-too-long usa-input-error-message selection-validation-message"> Name must be less than 100 characters </div></div><div class="action-panel-control-wrap existing-collections-controls"><label for="collections-action-dialog-add-to-existing" class="action-panel-label"> Choose a collection: </label><select id="collections-action-dialog-add-to-existing" class="action-panel-selector collections-action-add-to-existing" data-ga-category="collections_button" data-ga-action="select_collection" data-ga-label="($('.collections-action-add-to-existing').val() === 'Favorites') ? 'Favorites' : 'non_favorites_collection'"></select><div class="collections-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your collection due to an error<br><a href="#">Please try again</a></div></div></div><div class="action-panel-actions"><button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore data-ga-category="collections_button" data-ga-action="click" data-ga-label="add"> Add </button><button class="action-panel-cancel" aria-label="Close 'Add to Collections' panel" ref="linksrc=close_collections_panel" aria-controls="collections-action-panel" aria-expanded="false" data-ga-category="collections_button" data-ga-action="click" data-ga-label="cancel"> Cancel </button></div></form></div></div></div></div><button class="more-actions-button more-actions-dialog-trigger" title="Open dialog with more actions to take" ref="linksrc=more_actions_btn"></button><div class="display-options" 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ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Alaina M Richard">Alaina M Richard</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Chemical Biology Program, University of Michigan, Ann Arbor, MI 48109, USA." href="#full-view-affiliation-1" ref="linksrc=author_aff"> 1 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Estrada+DF&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="D Fernando Estrada">D Fernando Estrada</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, Kansas 66045, USA." href="#full-view-affiliation-2" ref="linksrc=author_aff"> 2 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Flynn+L&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Liam Flynn">Liam Flynn</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu." href="#full-view-affiliation-3" ref="linksrc=author_aff"> 3 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Pochapsky+SS&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Susan Sondej Pochapsky">Susan Sondej Pochapsky</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu." href="#full-view-affiliation-3" ref="linksrc=author_aff"> 3 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Scott+EE&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Emily E Scott">Emily E Scott</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Chemical Biology Program, University of Michigan, Ann Arbor, MI 48109, USA." href="#full-view-affiliation-1" ref="linksrc=author_aff"> 1 </a><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, Kansas 66045, USA." href="#full-view-affiliation-2" ref="linksrc=author_aff"> 2 </a><span class="author-sup-separator"> </span><a class="affiliation-link" title="Departments of Medicinal Chemistry, Pharmacology, and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA." href="#full-view-affiliation-4" ref="linksrc=author_aff"> 4 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Pochapsky+TC&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Thomas C Pochapsky">Thomas C Pochapsky</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu." href="#full-view-affiliation-3" ref="linksrc=author_aff"> 3 </a><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Biochemistry and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA." href="#full-view-affiliation-5" 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66045, USA.</li> <li data-affiliation-id="full-view-affiliation-3" id="full-view-affiliation-3" ><sup class="key">3</sup> Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu.</li> <li data-affiliation-id="full-view-affiliation-4" id="full-view-affiliation-4" ><sup class="key">4</sup> Departments of Medicinal Chemistry, Pharmacology, and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.</li> <li data-affiliation-id="full-view-affiliation-5" id="full-view-affiliation-5" ><sup class="key">5</sup> Department of Biochemistry and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA.</li> </ul> </div> </div> </div> <ul class="identifiers" id="full-view-identifiers"> <li> <span class="identifier pubmed"> <span class="id-label"> PMID: </span> <strong class="current-id" title="PubMed ID">38842434</strong> </span> </li> <li> <span class="identifier pmc"> 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class="authors-list"> <span class="authors-list-item "><span class="full-name">Alaina M Richard</span><span class="citation-separator"> et al.</span></span> </span> <span class="citation-journal"> Phys Chem Chem Phys<span class="citation-separator">.</span> </span> <span class="date"> <time class="citation-year" datetime="2024">2024</time><span class="citation-separator">.</span> </span> </div> <div class="more-article-details-section with-display-settings"> <button class="more-article-details" id="toggle-article-details" data-alt-text="Hide details" ref="linksrc=toggle_article_details" aria-controls="full-article-details" aria-expanded="false" data-fetch-url="/38842434/long-authors/" data-id-prefix=""> <span class="title">Show details</span> </button> <div class="display-options" data-format-key="format" data-default-format-value="abstract" data-current-format-value="abstract" role="region"> <button class="trigger usa-button" title="Change format" data-ga-category="display_options" 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class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Chemical Biology Program, University of Michigan, Ann Arbor, MI 48109, USA." href="#short-view-affiliation-1" ref="linksrc=author_aff"> 1 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Estrada+DF&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="D Fernando Estrada">D Fernando Estrada</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, Kansas 66045, USA." href="#short-view-affiliation-2" ref="linksrc=author_aff"> 2 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Flynn+L&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Liam Flynn">Liam Flynn</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu." href="#short-view-affiliation-3" ref="linksrc=author_aff"> 3 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Pochapsky+SS&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Susan Sondej Pochapsky">Susan Sondej Pochapsky</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu." href="#short-view-affiliation-3" ref="linksrc=author_aff"> 3 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Scott+EE&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Emily E Scott">Emily E Scott</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Chemical Biology Program, University of Michigan, Ann Arbor, MI 48109, USA." href="#short-view-affiliation-1" ref="linksrc=author_aff"> 1 </a><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, Kansas 66045, USA." href="#short-view-affiliation-2" ref="linksrc=author_aff"> 2 </a><span class="author-sup-separator"> </span><a class="affiliation-link" title="Departments of Medicinal Chemistry, Pharmacology, and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA." href="#short-view-affiliation-4" ref="linksrc=author_aff"> 4 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Pochapsky+TC&cauthor_id=38842434" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Thomas C Pochapsky">Thomas C Pochapsky</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu." href="#short-view-affiliation-3" ref="linksrc=author_aff"> 3 </a><span class="author-sup-separator"> </span><a class="affiliation-link" title="Department of Biochemistry and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA." href="#short-view-affiliation-5" ref="linksrc=author_aff"> 5 </a></sup></span> </div> </div> <div class="affiliations"> <h3 class="title"> Affiliations </h3> <ul class="item-list"> <li data-affiliation-id="short-view-affiliation-1" id="short-view-affiliation-1" ><sup class="key">1</sup> Chemical Biology Program, University of Michigan, Ann Arbor, MI 48109, USA.</li> <li data-affiliation-id="short-view-affiliation-2" id="short-view-affiliation-2" ><sup class="key">2</sup> Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, Kansas 66045, USA.</li> <li data-affiliation-id="short-view-affiliation-3" id="short-view-affiliation-3" ><sup class="key">3</sup> Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA. pochapsk@brandeis.edu.</li> <li data-affiliation-id="short-view-affiliation-4" id="short-view-affiliation-4" ><sup class="key">4</sup> Departments of Medicinal Chemistry, Pharmacology, and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.</li> <li data-affiliation-id="short-view-affiliation-5" id="short-view-affiliation-5" ><sup class="key">5</sup> Department of Biochemistry and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South St., Waltham, MA 02454-9110, USA.</li> </ul> </div> </div> <ul class="identifiers" id="short-view-identifiers"> <li> <span class="identifier pubmed"> <span class="id-label"> PMID: </span> <strong class="current-id" title="PubMed ID">38842434</strong> </span> </li> <li> <span class="identifier pmc"> <span class="id-label"> PMCID: </span> <a class="id-link" target="_blank" rel="noopener" ref="linksrc=article_id_link&article_id=PMC11186455&id_type=PMC" href="https://pmc.ncbi.nlm.nih.gov/articles/PMC11186455/" data-ga-category="full_text" data-ga-action="PMCID" > PMC11186455 </a> </span> </li> <li> <span class="identifier doi"> <span class="id-label"> DOI: </span> <a class="id-link" target="_blank" rel="noopener" ref="linksrc=article_id_link&article_id=10.1039/d4cp01268b&id_type=DOI" href="https://doi.org/10.1039/d4cp01268b" data-ga-category="full_text" data-ga-action="DOI" > 10.1039/d4cp01268b </a> </span> </li> </ul> <div class="in-clipboard-label " hidden> Item in Clipboard </div> </div> </div> </header> <div class="actions-buttons inline"><div class="inner-wrap"><button class="full-text-links-button full-text-links-dialog-trigger" title="Open dialog with full text links" ref="linksrc=full_text_links"> Full text links </button><button class="citation-button citation-dialog-trigger" aria-label="Open dialog with citation text in different styles" data-ga-category="save_share" data-ga-action="cite" data-ga-label="open" data-all-citations-url="/38842434/citations/" data-citation-style="nlm" data-pubmed-format-link="/38842434/export/"><span class="button-label">Cite</span></button><button class="more-actions-button more-actions-dialog-trigger" title="Open dialog with more actions to take" ref="linksrc=more_actions_btn"></button><div class="display-options" data-format-key="format" data-default-format-value="abstract" data-current-format-value="abstract" role="region"><button class="trigger usa-button" title="Change format" data-ga-category="display_options" data-ga-action="display_options_dialog"><span class="button-label">Display options</span></button><div class="dropdown" hidden><div class="title"> Display options </div><div class="content"><div class="format-container"><span class="option-label"> Format </span><select name="format" data-initial-value="abstract" aria-label="Display format" id="inline-actions-display-format"><option value="abstract">Abstract</option><option value="pubmed">PubMed</option><option value="pmid">PMID</option></select></div></div></div></div></div></div> <div class="abstract" id="abstract"> <h2 class="title"> Abstract </h2> <div class="abstract-content selected" id="eng-abstract"> <p> The human steroidogenic cytochrome P450 CYP17A1 catalyzes two types of reactions in the biosynthetic pathway leading from pregnenolone to testosterone and several other steroid hormones. The first is the hydroxylation of pregnenolone or progesterone to the corresponding 17α-hydroxy steroid, followed by a lyase reaction that converts these 17α-hydroxy intermediates to the androgens dehydroepiandrosterone and androstenedione, respectively. cytochrome <i>b</i><sub>5</sub> (cyt<i>b</i><sub>5</sub>) is known to act as both an effector and electron donor for the lyase oxidations, markedly stimulating the rate of the lyase reaction in its presence relative to the rate in its absence. Extensive sequential backbone <sup>1</sup>H,<sup>15</sup>N and <sup>13</sup>C nuclear magnetic resonance assignments have now been made for oxidized CYP17A1 bound to the prostate cancer drug and inhibitor abiraterone. This is the first eukaryotic P450 for which such assignments are now available. These assignments allow more complete interpretation of the structural perturbations observed upon cyt<i>b</i><sub>5</sub> addition. Possible mechanism(s) for the effector activity of cyt<i>b</i><sub>5</sub> are discussed in light of this new information. </p> </div> </div> <p class="disclaimer" id="disclaimer"> <a href="/disclaimer/" target="_blank" data-ga-category="literature_resources" data-ga-action="disclaimer_link" data-ga-label="under_abstract">PubMed Disclaimer</a> </p> <div class="conflict-of-interest" id="conflict-of-interest"> <h2 class="title"> Conflict of interest statement </h2> <div class="statement"> <p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one">There are no conflicts to declare.</p> </div> </div> <div class="figures" id="figures"> <h2 class="title"> Figures </h2> <div class="figures-list" id="slides-container" itemscope itemtype="http://schema.org/ImageGallery"> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="0" data-label-slug="fig-1"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/8ee6bb4e74da/d4cp01268b-f1.jpg" itemprop="contentUrl" aria-describedby="figure-caption-0" role="button" data-image-width="784" data-image-height="1042" data-image-alt="Fig. 1" data-pmc-id="PMC11186455" data-figure-id="fig1"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-0" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/f15a1b46b528/d4cp01268b-f1.gif" alt="Fig. 1" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="784"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1042"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 1. Roles of CYP17A1 in steroid… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 1. Roles of CYP17A1 in steroid biosynthetic pathway leading to testosterone, estrogens, glucocorticoids, and… </p> </strong> </div> <figcaption id="figure-caption-0" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 1. Roles of CYP17A1 in steroid biosynthetic pathway leading to testosterone, estrogens, glucocorticoids, and mineralocorticoids. 3β-HSD (3-beta-hydroxysteroid dehydrogenase) converts 1̲ to 4̲. </strong> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="1" data-label-slug="fig-2"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/1e3214c7219e/d4cp01268b-f2.jpg" itemprop="contentUrl" aria-describedby="figure-caption-1" role="button" data-image-width="784" data-image-height="855" data-image-alt="Fig. 2" data-pmc-id="PMC11186455" data-figure-id="fig2"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-1" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/f107eef79a14/d4cp01268b-f2.gif" alt="Fig. 2" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="784"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="855"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 2. Strip from <sup> 15 </sup> N-edited <sup> 1… </sup> </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 2. Strip from <sup> 15 </sup> N-edited <sup> 1 </sup> H, <sup> 1 </sup> H NOESY of <sup> 2 </sup> H, <sup> 13… </sup> </p> </strong> </div> <figcaption id="figure-caption-1" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 2. Strip from <sup>15</sup>N-edited <sup>1</sup>H, <sup>1</sup>H NOESY of <sup>2</sup>H, <sup>13</sup>C, <sup>15</sup>N CYP17A1/abiraterone showing NOE connectivity between the amide NH resonance of I493 and those of residues G162, Q163 and I165 in the β5 sheet. Positions of those residues in the <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="3RUK">3RUK</ext-link> structure as well as N–N distances are shown at right. </strong> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="2" data-label-slug="fig-3"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/f47ae60cc923/d4cp01268b-f3.jpg" itemprop="contentUrl" aria-describedby="figure-caption-2" role="button" data-image-width="720" data-image-height="858" data-image-alt="Fig. 3" data-pmc-id="PMC11186455" data-figure-id="fig3"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-2" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/94e94a0499a6/d4cp01268b-f3.gif" alt="Fig. 3" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="720"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="858"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 3. CYP17A1 (PDB entry <ext-link ext-link-type="uri" xlink:href="3RUK" xmlns:xlink="http://www.w3.org/1999/xlink"> 3RUK </ext-link> )… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 3. CYP17A1 (PDB entry <ext-link ext-link-type="uri" xlink:href="3RUK" xmlns:xlink="http://www.w3.org/1999/xlink"> 3RUK </ext-link> ) with abiraterone bound, showing distribution of sequential backbone… </p> </strong> </div> <figcaption id="figure-caption-2" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 3. CYP17A1 (PDB entry <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="3RUK">3RUK</ext-link>) with abiraterone bound, showing distribution of sequential backbone resonance assignments. Assignments for regions in green are supported by multiple forms of data (see text for details), regions in blue are provisionally assigned based on fewer data. Those shown in grey are not assigned. Secondary structures were assigned by DSSP analysis of PDB <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="3RUK">3RUK</ext-link> (see ESI‡) are labeled as first described by Poulos. Helices: A, M49–Y60; B, H80–L86; B′, A100–A105; C, A119–A133; D, L142–T159; E, S168–F184; F, E194–L209; G′, W120–I123; G, T228–K253; H, M262–N272; I, D289–H320; J, P322–N335; K, L351–L363; K′, L396–H401; K′′, P414–F417; L, E445–R462. Strands: β1, I63–M68, K71–V76, E391–I394, H373–K374; β2, S379–I381, F384–386; β3, F463–E466, K492–V495; β4, I479–P480, F484–L385; β5, F463–Q466, V491–495. Abiraterone is shown in thin red lines, heme is shown in heavier pink lines. </strong> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="3" data-label-slug="fig-4"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/6888b0cccf35/d4cp01268b-f4.jpg" itemprop="contentUrl" aria-describedby="figure-caption-3" role="button" data-image-width="750" data-image-height="507" data-image-alt="Fig. 4" data-pmc-id="PMC11186455" data-figure-id="fig4"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-3" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/53e2ec5164bc/d4cp01268b-f4.gif" alt="Fig. 4" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="750"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="507"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 4. (left) Distribution of doubled resonances… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 4. (left) Distribution of doubled resonances (in purple) indicating slow exchange of two conformational… </p> </strong> </div> <figcaption id="figure-caption-3" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 4. (left) Distribution of doubled resonances (in purple) indicating slow exchange of two conformational ensembles mapped on the CYP17A1/abiraterone X-ray structure (<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="3RUK">3RUK</ext-link>). The F–G loop and K′ helix shown in orange show intermediate exchange, as evidenced by significant broadening of resonances. (right) Examples of doubling of correlations in <sup>1</sup>H, <sup>15</sup>N-TROSY HSQC spectrum of CYP17A1/abiraterone, Asp 139 (C–D loop), Ser 187 (E–F loop) and Trp 499 (β5 strand). </strong> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="4" data-label-slug="fig-5"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/7e4d44f52ff4/d4cp01268b-f5.jpg" itemprop="contentUrl" aria-describedby="figure-caption-4" role="button" data-image-width="784" data-image-height="522" data-image-alt="Fig. 5" data-pmc-id="PMC11186455" data-figure-id="fig5"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-4" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/4cf673dae7e3/d4cp01268b-f5.gif" alt="Fig. 5" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="784"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="522"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 5. (left) Conformational exchange detected in… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 5. (left) Conformational exchange detected in <sup> 15 </sup> N-edited NOESY of CYP17A1/abiraterone for Gly 138… </p> </strong> </div> <figcaption id="figure-caption-4" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 5. (left) Conformational exchange detected in <sup>15</sup>N-edited NOESY of CYP17A1/abiraterone for Gly 138 (C–D loop). Autocorrelation peaks for G138 and G138′ are indicated, as are exchange cross peaks. (right) Collapse of states observed in CYP17A1/abiraterone <sup>1</sup>H, <sup>15</sup>N-TROSY HSQC for Gly 138 in the absence of cyt<i>b</i><sub>5</sub> (blue) to a single conformation (red) upon addition of 0.4 equivalents of cyt<i>b</i><sub>5</sub>. </strong> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="5" data-label-slug="fig-6"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/d1e18536fee3/d4cp01268b-f6.jpg" itemprop="contentUrl" aria-describedby="figure-caption-5" role="button" data-image-width="784" data-image-height="1626" data-image-alt="Fig. 6" data-pmc-id="PMC11186455" data-figure-id="fig6"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-5" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/6084d7203c78/d4cp01268b-f6.gif" alt="Fig. 6" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="784"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1626"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 6. Preferential population of CYP17A1/abiraterone major… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 6. Preferential population of CYP17A1/abiraterone major conformation upon binding of cyt <i> b </i> <sub> 5 </sub> .… </p> </strong> </div> <figcaption id="figure-caption-5" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 6. Preferential population of CYP17A1/abiraterone major conformation upon binding of cyt<i>b</i><sub>5</sub>. <sup>1</sup>H–<sup>15</sup>N correlations for Asp 139 (C–D loop), Thr 423 (β-meander) and Trp 499 (β5 strand) in the absence (blue) and presence (red) of 0.4 equivalents of cyt<i>b</i><sub>5</sub>. </strong> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="6" data-label-slug="fig-7"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/a55a5c8eb11b/d4cp01268b-f7.jpg" itemprop="contentUrl" aria-describedby="figure-caption-6" role="button" data-image-width="720" data-image-height="709" data-image-alt="Fig. 7" data-pmc-id="PMC11186455" data-figure-id="fig7"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-6" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/2a4c58fa782d/d4cp01268b-f7.gif" alt="Fig. 7" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="720"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="709"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 7. Distribution of regions (in red)… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 7. Distribution of regions (in red) showing conformational selection in CYP17A1/abiraterone upon binding of… </p> </strong> </div> <figcaption id="figure-caption-6" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 7. Distribution of regions (in red) showing conformational selection in CYP17A1/abiraterone upon binding of cyt<i>b</i><sub>5</sub>. The view is of the proximal surface, rotated ∼60° counterclockwise from Fig. 3. Regions shown in orange are tentative, either due to spectral overlap or provisional resonance assignment. The side chains of arginine residues (R347, R358 and R449) implicated in cyt<i>b</i><sub>5</sub> binding are shown in red. </strong> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="7" data-label-slug="fig-8"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/b2470410a8d6/d4cp01268b-f8.jpg" itemprop="contentUrl" aria-describedby="figure-caption-7" role="button" data-image-width="784" data-image-height="660" data-image-alt="Fig. 8" data-pmc-id="PMC11186455" data-figure-id="fig8"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-7" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/951e7bfc061c/d4cp01268b-f8.gif" alt="Fig. 8" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="784"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="660"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 8. (right) Overlay of <sup> 1 </sup> H–… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 8. (right) Overlay of <sup> 1 </sup> H– <sup> 15 </sup> N correlation for Arg 347 in the… </p> </strong> </div> <figcaption id="figure-caption-7" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 8. (right) Overlay of <sup>1</sup>H–<sup>15</sup>N correlation for Arg 347 in the absence (light contours) and presence (dark contours) of 0.4 equivalents of cyt<i>b</i><sub>5</sub>. (left) Spectral subtraction of the same correlation with black contours representing loss of signal and orange showing increased intensity upon addition of cyt<i>b</i><sub>5</sub>. </strong> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="8" data-label-slug="fig-9"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/5f87997ad449/d4cp01268b-f9.jpg" itemprop="contentUrl" aria-describedby="figure-caption-8" role="button" data-image-width="783" data-image-height="667" data-image-alt="Fig. 9" data-pmc-id="PMC11186455" data-figure-id="fig9"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-8" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db66/11186455/488fd8fc0229/d4cp01268b-f9.gif" alt="Fig. 9" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="783"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="667"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Fig. 9. Model for a complex formed… </p> </strong> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Fig. 9. Model for a complex formed by cyt <i> b </i> <sub> 5 </sub> on the proximal face… </p> </strong> </div> <figcaption id="figure-caption-8" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Fig. 9. Model for a complex formed by cyt<i>b</i><sub>5</sub> on the proximal face of CYP17A1, placing Arg 347, Arg 358 and Arg 449 adjacent to Glu 44, Glu 43 and a cyt<i>b</i><sub>5</sub> heme propionate, respectively. Both heme iron atoms are shown as orange spheres. CYP17A1 is rotated counterclockwise ∼90° and tilted clockwise ∼20° from the orientation in Fig. 3 membrane binding domains of both proteins are not shown, but are extensions of the C-term of cyt<i>b</i><sub>5</sub> and N-term of CYP17A1 that are oriented toward the heavy black line at the bottom of the figure. Crystal structures <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="1ICC">1ICC</ext-link> and <ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="3RUK">3RUK</ext-link> were used as templates. Model is courtesy of Prof. Eliana Asciutto, UNSAM, Buenos Aires, AG. </strong> </figcaption> </figure> </div> <div class="see-all actions-bar"> <button class="show-more" aria-controls="slides-container" data-ga-category="figure" data-ga-action="show_all"> All figures (9) </button> </div>  <div class="pswp figures-dialog" id="figures-dialog" tabindex="-1" role="dialog" aria-hidden="true" data-image-gallery-uid="article-figures" aria-label="Figures Dialog">  <div class="pswp__bg"></div>  <div class="pswp__scroll-wrap">  <div class="pswp__container"> <div class="pswp__item"></div> <div class="pswp__item"></div> <div class="pswp__item"></div> </div>  <div class="pswp__ui pswp__ui--hidden"> <div class="pswp__top-bar">  <div class="pswp__counter"></div> <button class="pswp__button pswp__button--close" title="Close (Esc)"></button> <button class="pswp__button pswp__button--share" title="Share"></button> <button class="pswp__button pswp__button--fs" title="Toggle fullscreen"></button> <button class="pswp__button pswp__button--zoom" title="Zoom in/out"></button>   <div class="pswp__preloader"> <div class="pswp__preloader__icn"> <div class="pswp__preloader__cut"> <div class="pswp__preloader__donut"></div> </div> </div> </div> </div> <div class="pswp__share-modal pswp__share-modal--hidden pswp__single-tap"> <div class="pswp__share-tooltip"></div> </div> <button class="pswp__button pswp__button--arrow--left" title="Previous (arrow left)"> </button> <button class="pswp__button pswp__button--arrow--right" title="Next (arrow right)"> </button> <div class="pswp__caption"> <div class="pswp__caption__center"></div> </div> </div> </div> </div> <div class="figure-dialog-caption"> <div class="figure-caption-link-container"> <a class="figure-caption-link" href="#">See this image and copyright information in PMC</a> </div> <div class="figure-caption-center" data-expand-title="click to show full caption" data-collapse-title="click to hide full caption"> </div> <button class="figure-caption-toggle-btn" aria-label="show full caption" data-expand-text="show full caption" data-collapse-text="hide full caption">  </button> </div> </div> <div class="similar-articles" id="similar"> <h2 class="title"> Similar articles </h2> <ul class="articles-list" id="similar-articles-list"> <li class="full-docsum"> <div class="docsum-content" style="display:inline;"> <a class="docsum-title" href="/24671419/" ref="article_id=24671419&linksrc=similar_articles_link&ordinalpos=1" data-ga-category="similar_article" data-ga-action="24671419" data-ga-label=""> Human cytochrome P450 17A1 conformational selection: modulation by ligand and cytochrome b5. </a> <div class="docsum-citation full-citation"> <span class="docsum-authors full-authors">Estrada DF, Skinner AL, Laurence JS, Scott EE.</span> <span class="docsum-authors short-authors">Estrada DF, et al.</span> <span class="docsum-journal-citation full-journal-citation">J Biol Chem. 2014 May 16;289(20):14310-20. doi: 10.1074/jbc.M114.560144. 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Cytochromes b5" tabindex="0" aria-controls="keyword-actions-substances-1" aria-expanded="false" data-pinger-ignore> Cytochromes b5 </button><div id="keyword-actions-substances-1" class="keyword-actions-dropdown dropdown dropdown-container" hidden aria-label="Dropdown menu for keyword Cytochromes b5"><div class="title">Actions</div><div class="content"><ul class="keyword-actions-links"><li><a class="search-in-pubmed-link dropdown-block-link" href="/?term=%22Cytochromes+b5%22%5Bnm%5D&sort=date&sort_order=desc" data-href="/?term=%22Cytochromes+b5%22%5Bnm%5D&sort=date&sort_order=desc" ref="linksrc=search_in_pubmed_keyword_link&keyword="Cytochromes b5"[nm]" data-ga-category="search" data-ga-action="substance" data-ga-label="Cytochromes b5"> Search in PubMed </a></li><li><a class="search-in-mesh-link dropdown-block-link" ref="linksrc=search_in_mesh_link" href="https://www.ncbi.nlm.nih.gov/mesh?term=%22Cytochromes+b5%22" data-ga-category="search_mesh" data-ga-action="substance_link" 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Nucleotide </a></li><li><a title="Published protein sequences" href="https://www.ncbi.nlm.nih.gov/protein?linkname=pubmed_protein&from_uid=38842434" ref="log$=recordlinks&logdbfrom=pubmed&ncbi_uid=38842434&ordinalpos=1&linkpos=5"> Protein </a></li><li><a title="Link to Protein RefSeqs" href="https://www.ncbi.nlm.nih.gov/protein?linkname=pubmed_protein_refseq&from_uid=38842434" ref="log$=recordlinks&logdbfrom=pubmed&ncbi_uid=38842434&ordinalpos=1&linkpos=6"> Protein (RefSeq) </a></li><li><a title="Related PubChem Compound via MeSH" href="https://www.ncbi.nlm.nih.gov/pccompound?linkname=pubmed_pccompound_mesh&from_uid=38842434" ref="log$=recordlinks&logdbfrom=pubmed&ncbi_uid=38842434&ordinalpos=1&linkpos=7"> PubChem Compound (MeSH Keyword) </a></li></ul> </div> <div id="grants" class="grants"> <h2 class="title"> Grants and funding </h2> <div class="grants-list"><ul id="grants-head-list"><li class="grant-item"><a title="All articles for grant P20 GM103420/GM/NIGMS NIH HHS/United States" 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