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Krieger and Dougherty expression</title> <meta name="description" content="Search results for: modified Krieger and Dougherty expression"> <meta name="keywords" content="modified Krieger and Dougherty expression"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open 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class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="modified Krieger and Dougherty expression"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4426</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: modified Krieger and Dougherty expression</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4426</span> Rheological and Morphological Properties of Investment Casting Pattern Material Based on Paraffin Wax Fortified with Linear Low-Density Polyethylene and Filled with Poly Methyl Methacrylate</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Robert%20Kimutai%20Tewo">Robert Kimutai Tewo</a>, <a href="https://publications.waset.org/abstracts/search?q=Hilary%20Limo%20Rutto"> Hilary Limo Rutto</a>, <a href="https://publications.waset.org/abstracts/search?q=Tumisang%20Seodigeng"> Tumisang Seodigeng</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The rheological and morphological properties of paraffin wax, linear low-density polyethylene (LLDPE), and poly (methyl methacrylate) (PMMA) microbeads formulations were prepared via an extrusion process. The blends were characterized by rheometry, scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopy. The results indicated that the viscosity of the blends increased as compared to that of neat wax. SEM confirmed that LLDPE alters the wax crystal habit at higher concentrations. The rheological experimental data fitted with predicted data using the modified Krieger and Dougherty expression. The SEM micrograph of wax/LLDPE/PMMA revealed a near-perfect spherical nature for the filler particles in the wax/EVA polymer matrix. The FT-IR spectra show the deformation vibrations stretch of a long-chain aliphatic hydrocarbon (C-H) and also the presence of carbonyls absorption group denoted by -C=O- stretch. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=investment%20casting%20pattern" title="investment casting pattern">investment casting pattern</a>, <a href="https://publications.waset.org/abstracts/search?q=paraffin%20wax" title=" paraffin wax"> paraffin wax</a>, <a href="https://publications.waset.org/abstracts/search?q=LLDPE" title=" LLDPE"> LLDPE</a>, <a href="https://publications.waset.org/abstracts/search?q=PMMA" title=" PMMA"> PMMA</a>, <a href="https://publications.waset.org/abstracts/search?q=rheological%20properties" title=" rheological properties"> rheological properties</a>, <a href="https://publications.waset.org/abstracts/search?q=modified%20Krieger%20and%20Dougherty%20expression" title=" modified Krieger and Dougherty expression"> modified Krieger and Dougherty expression</a> </p> <a href="https://publications.waset.org/abstracts/116320/rheological-and-morphological-properties-of-investment-casting-pattern-material-based-on-paraffin-wax-fortified-with-linear-low-density-polyethylene-and-filled-with-poly-methyl-methacrylate" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116320.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">170</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4425</span> Influence of the Granular Mixture Properties on the Rheological Properties of Concrete: Yield Stress Determination Using Modified Chateau et al. Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rachid%20Zentar">Rachid Zentar</a>, <a href="https://publications.waset.org/abstracts/search?q=Mokrane%20Bala"> Mokrane Bala</a>, <a href="https://publications.waset.org/abstracts/search?q=Pascal%20Boustingorry"> Pascal Boustingorry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The prediction of the rheological behavior of concrete is at the center of current concerns of the concrete industry for different reasons. The shortage of good quality standard materials combined with variable properties of available materials imposes to improve existing models to take into account these variations at the design stage of concrete. The main reasons for improving the predictive models are, of course, saving time and cost at the design stage as well as to optimize concrete performances. In this study, we will highlight the different properties of the granular mixtures that affect the rheological properties of concrete. Our objective is to identify the intrinsic parameters of the aggregates which make it possible to predict the yield stress of concrete. The work was done using two typologies of grains: crushed and rolled aggregates. The experimental results have shown that the rheology of concrete is improved by increasing the packing density of the granular mixture using rolled aggregates. The experimental program realized allowed to model the yield stress of concrete by a modified model of Chateau et al. through a dimensionless parameter following Krieger-Dougherty law. The modelling confirms that the yield stress of concrete depends not only on the properties of cement paste but also on the packing density of the granular skeleton and the shape of grains. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=crushed%20aggregates" title="crushed aggregates">crushed aggregates</a>, <a href="https://publications.waset.org/abstracts/search?q=intrinsic%20viscosity" title=" intrinsic viscosity"> intrinsic viscosity</a>, <a href="https://publications.waset.org/abstracts/search?q=packing%20density" title=" packing density"> packing density</a>, <a href="https://publications.waset.org/abstracts/search?q=rolled%20aggregates" title=" rolled aggregates"> rolled aggregates</a>, <a href="https://publications.waset.org/abstracts/search?q=slump" title=" slump"> slump</a>, <a href="https://publications.waset.org/abstracts/search?q=yield%20stress%20of%20concrete" title=" yield stress of concrete"> yield stress of concrete</a> </p> <a href="https://publications.waset.org/abstracts/108020/influence-of-the-granular-mixture-properties-on-the-rheological-properties-of-concrete-yield-stress-determination-using-modified-chateau-et-al-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/108020.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">127</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4424</span> Time-Domain Expressions for Bridge Self-Excited Aerodynamic Forces by Modified Particle Swarm Optimizer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hao-Su%20Liu">Hao-Su Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun-Qing%20Lei"> Jun-Qing Lei</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study introduces the theory of modified particle swarm optimizer and its application in time-domain expressions for bridge self-excited aerodynamic forces. Based on the indicial function expression and the rational function expression in time-domain expression for bridge self-excited aerodynamic forces, the characteristics of the two methods, i.e. the modified particle swarm optimizer and conventional search method, are compared in flutter derivatives’ fitting process. Theoretical analysis and numerical results indicate that adopting whether the indicial function expression or the rational function expression, the fitting flutter derivatives obtained by modified particle swarm optimizer have better goodness of fit with ones obtained from experiment. As to the flutter derivatives which have higher nonlinearity, the self-excited aerodynamic forces, using the flutter derivatives obtained through modified particle swarm optimizer fitting process, are much closer to the ones simulated by the experimental. The modified particle swarm optimizer was used to recognize the parameters of time-domain expressions for flutter derivatives of an actual long-span highway-railway truss bridge with double decks at the wind attack angle of 0°, -3° and +3°. It was found that this method could solve the bounded problems of attenuation coefficient effectively in conventional search method, and had the ability of searching in unboundedly area. Accordingly, this study provides a method for engineering industry to frequently and efficiently obtain the time-domain expressions for bridge self-excited aerodynamic forces. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=time-domain%20expressions" title="time-domain expressions">time-domain expressions</a>, <a href="https://publications.waset.org/abstracts/search?q=bridge%20self-excited%20aerodynamic%20forces" title=" bridge self-excited aerodynamic forces"> bridge self-excited aerodynamic forces</a>, <a href="https://publications.waset.org/abstracts/search?q=modified%20particle%20swarm%20optimizer" title=" modified particle swarm optimizer"> modified particle swarm optimizer</a>, <a href="https://publications.waset.org/abstracts/search?q=long-span%20highway-railway%20truss%20bridge" title=" long-span highway-railway truss bridge"> long-span highway-railway truss bridge</a> </p> <a href="https://publications.waset.org/abstracts/69848/time-domain-expressions-for-bridge-self-excited-aerodynamic-forces-by-modified-particle-swarm-optimizer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/69848.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">314</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4423</span> Cloning, Expression and N-Terminal Pegylation of Human Interferon Alpha-2b Analogs and Their Cytotoxic Evaluation against Cancer Cell Lines</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Syeda%20Kiran%20Shahzadi">Syeda Kiran Shahzadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Nasir%20Mahmood"> Nasir Mahmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Abdul%20Qadir"> Muhammad Abdul Qadir</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the current research, three recombinant human interferon alpha-2b proteins (two modified and one normal form) were produced and Pegylated with an aim to produce more effective drugs against viral infections and cancers. The modified recombinant human interferon alpha-2b proteins were produced by site-directed modifications of interferon alpha 2b gene, targeting the amino acids at positions ‘R23’ and ‘H34’. The resulting chemically modified and unmodified forms of human interferon alpha 2b were conjugated with methoxy-polyethylene glycol propanealdehyde (400 KDa) and methoxy-polyethylene glycol succinimidyl succinate (400 KDa). Pegylation of normal and modified forms of Interferon alpha-2b prolong their release time and enhance their efficacy. The conjugation of PEG with modified and unmodified human interferon alpha 2b protein drugs was also characterized with 1H-NMR, HPLC, and SDS-PAGE. Antiproliferative assays of modified and unmodified forms of drugs were performed in cell based bioassays using MDBK cell lines. The results indicated that experimentally produced recombinant human interferon alpha-2b proteins were biologically active and resulted in significant inhibition of cell growth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=protein%20refolding" title="protein refolding">protein refolding</a>, <a href="https://publications.waset.org/abstracts/search?q=antiproliferative%20activities" title=" antiproliferative activities"> antiproliferative activities</a>, <a href="https://publications.waset.org/abstracts/search?q=biomedical%20applications" title=" biomedical applications"> biomedical applications</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20interferon%20alpha-2b" title=" human interferon alpha-2b"> human interferon alpha-2b</a>, <a href="https://publications.waset.org/abstracts/search?q=pegylation" title=" pegylation"> pegylation</a>, <a href="https://publications.waset.org/abstracts/search?q=mPEG-propionaldehyde" title=" mPEG-propionaldehyde"> mPEG-propionaldehyde</a>, <a href="https://publications.waset.org/abstracts/search?q=site%20directed%20mutagenesis" title=" site directed mutagenesis"> site directed mutagenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20coli%20expression" title=" E. coli expression"> E. coli expression</a> </p> <a href="https://publications.waset.org/abstracts/83956/cloning-expression-and-n-terminal-pegylation-of-human-interferon-alpha-2b-analogs-and-their-cytotoxic-evaluation-against-cancer-cell-lines" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/83956.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">177</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4422</span> Chemical Synthesis of a cDNA and Its Expression Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Salman%20Akrokayan">Salman Akrokayan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Synthetic cDNA (ScDNA) of granulocyte colony-stimulating factor (G-CSF) was constructed using a DNA synthesizer with the aim to increase its expression level. 5' end of the ScDNA of G-CSF coding region was modified by decreasing the GC content without altering the predicted amino acids sequence. The identity of the resulting protein from ScDNA was confirmed by the highly specific enzyme-linked immunosorbent assay. In conclusion, a synthetic G-CSF cDNA in combination with the recombinant DNA protocol offers a rapid and reliable strategy for synthesizing the target protein. However, the commercial utilization of this methodology requires rigorous validation and quality control. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=synthetic%20cDNA" title="synthetic cDNA">synthetic cDNA</a>, <a href="https://publications.waset.org/abstracts/search?q=recombinant%20G-CSF" title=" recombinant G-CSF"> recombinant G-CSF</a>, <a href="https://publications.waset.org/abstracts/search?q=cloning" title=" cloning"> cloning</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20expression" title=" gene expression"> gene expression</a> </p> <a href="https://publications.waset.org/abstracts/59150/chemical-synthesis-of-a-cdna-and-its-expression-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59150.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">284</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4421</span> Rheological Study of Natural Sediments: Application in Filling of Estuaries</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Serhal">S. Serhal</a>, <a href="https://publications.waset.org/abstracts/search?q=Y.%20Melinge"> Y. Melinge</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Rangeard"> D. Rangeard</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Hage%20Chehadeh"> F. Hage Chehadeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Filling of estuaries is an international problem that can cause economic and environmental damage. This work aims the study of the rheological structuring mechanisms of natural sedimentary liquid-solid mixture in estuaries in order to better understand their filling. The estuary of the Rance river, located in Brittany, France is particularly targeted by the study. The aim is to provide answers on the rheological behavior of natural sediments by detecting structural factors influencing the rheological parameters. So we can better understand the fillings estuarine areas and especially consider sustainable solutions of ‘cleansing’ of these areas. The sediments were collected from the trap of Lyvet in Rance estuary. This trap was created by the association COEUR (Comité Opérationnel des Elus et Usagers de la Rance) in 1996 in order to facilitate the cleansing of the estuary. It creates a privileged area for the deposition of sediments and consequently makes the cleansing of the estuary easier. We began our work with a preliminary study to establish the trend of the rheological behavior of the suspensions and to specify the dormant phase which precedes the beginning of the biochemical reactivity of the suspensions. Then we highlight the visco-plastic character at younger age using the Kinexus rheometer, plate-plate geometry. This rheological behavior of suspensions is represented by the Bingham model using dynamic yield stress and viscosity which can be a function of volume fraction, granular extent, and chemical reactivity. The evolution of the viscosity as a function of the solid volume fraction is modeled by the Krieger-Dougherty model. On the other hand, the analysis of the dynamic yield stress showed a fairly functional link with the solid volume fraction. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=estuaries" title="estuaries">estuaries</a>, <a href="https://publications.waset.org/abstracts/search?q=rheological%20behavior" title=" rheological behavior"> rheological behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=sediments" title=" sediments"> sediments</a>, <a href="https://publications.waset.org/abstracts/search?q=Kinexus%20rheometer" title=" Kinexus rheometer"> Kinexus rheometer</a>, <a href="https://publications.waset.org/abstracts/search?q=Bingham%20model" title=" Bingham model"> Bingham model</a>, <a href="https://publications.waset.org/abstracts/search?q=viscosity" title=" viscosity"> viscosity</a>, <a href="https://publications.waset.org/abstracts/search?q=yield%20stress" title=" yield stress"> yield stress</a> </p> <a href="https://publications.waset.org/abstracts/97288/rheological-study-of-natural-sediments-application-in-filling-of-estuaries" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/97288.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">160</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4420</span> Electrospun Nanofibrous Scaffolds Modified with Collagen-I and Fibronectin with LX-2 Cells to Study Liver Fibrosis in vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Prativa%20Das">Prativa Das</a>, <a href="https://publications.waset.org/abstracts/search?q=Lay%20Poh%20Tan"> Lay Poh Tan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Three-dimensional microenvironment is a need to study the event cascades of liver fibrosis in vitro. Electrospun nanofibers modified with essential extracellular matrix proteins can closely mimic the random fibrous structure of native liver extracellular matrix (ECM). In this study, we fabricate a series of 3D electrospun scaffolds by wet electrospinning process modified with different ratios of collagen-I to fibronectin to achieve optimized distribution of these two ECM proteins on the fiber surface. A ratio of 3:1 of collagen-I to fibronectin was found to be optimum for surface modification of electrospun poly(lactic-co-glycolic acid) (PLGA) fibers by chemisorption process. In 3:1 collagen-I to fibronectin modified scaffolds the total protein content increased by ~2 fold compared to collagen-I modified and ~1.5 fold compared to 1:1/9:1 collagen-I to fibronectin modified scaffolds. We have cultured LX-2 cells on this scaffold over 14 days and found that LX-2 cells acquired more quiescent phenotype throughout the culture period and shown significantly lower expression of alpha smooth muscle actin and collagen-I. Thus, this system can be used as a model to study liver fibrosis by using different fibrogenic mediators in vitro. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electrospinning" title="electrospinning">electrospinning</a>, <a href="https://publications.waset.org/abstracts/search?q=collagen-I%20and%20fibronectin" title=" collagen-I and fibronectin"> collagen-I and fibronectin</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20modification%20of%20fiber" title=" surface modification of fiber"> surface modification of fiber</a>, <a href="https://publications.waset.org/abstracts/search?q=LX-2%20cells" title=" LX-2 cells"> LX-2 cells</a>, <a href="https://publications.waset.org/abstracts/search?q=liver%20fibrosis" title=" liver fibrosis"> liver fibrosis</a> </p> <a href="https://publications.waset.org/abstracts/104340/electrospun-nanofibrous-scaffolds-modified-with-collagen-i-and-fibronectin-with-lx-2-cells-to-study-liver-fibrosis-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104340.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">126</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4419</span> Macronutrients and the FTO Gene Expression in Hypothalamus: A Systematic Review of Experimental Studies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saeid%20Doaei">Saeid Doaei</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The various studies have examined the relationship between FTO gene expression and macronutrients levels. In order to obtain better viewpoint from this interactions, all of the existing studies were reviewed systematically. All published papers have been obtained and reviewed using standard and sensitive keywords from databases such as CINAHL, Embase, PubMed, PsycInfo, and the Cochrane, from 1990 to 2016. The results indicated that all of 6 studies that met the inclusion criteria (from a total of 428 published article) found FTO gene expression changes at short-term follow-ups. Four of six studies found an increased FTO gene expression after calorie restriction, while two of them indicated decreased FTO gene expression. The effect of protein, carbohydrate and fat were separately assessed and suggested by all of six studies. In conclusion, the level of FTO gene expression in hypothalamus is related to macronutrients levels. Future research should evaluate the long-term impact of dietary interventions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=obesity" title="obesity">obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20expression" title=" gene expression"> gene expression</a>, <a href="https://publications.waset.org/abstracts/search?q=FTO" title=" FTO"> FTO</a>, <a href="https://publications.waset.org/abstracts/search?q=macronutrients" title=" macronutrients"> macronutrients</a> </p> <a href="https://publications.waset.org/abstracts/71018/macronutrients-and-the-fto-gene-expression-in-hypothalamus-a-systematic-review-of-experimental-studies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/71018.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">267</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4418</span> Practical Methods for Automatic MC/DC Test Cases Generation of Boolean Expressions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sekou%20Kangoye">Sekou Kangoye</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexis%20Todoskoff"> Alexis Todoskoff</a>, <a href="https://publications.waset.org/abstracts/search?q=Mihaela%20Barreau"> Mihaela Barreau</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Modified Condition/Decision Coverage (MC/DC) is a structural coverage criterion that aims to prove that all conditions involved in a Boolean expression can influence the result of that expression. In the context of automotive, MC/DC is highly recommended and even required for most security and safety applications testing. However, due to complex Boolean expressions that often embedded in those applications, generating a set of MC/DC compliant test cases for any of these expressions is a nontrivial task and can be time consuming for testers. In this paper we present an approach to automatically generate MC/DC test cases for any Boolean expression. We introduce novel techniques, essentially based on binary trees to quickly and optimally generate MC/DC test cases for the expressions. Thus, the approach can be used to reduce the manual testing effort of testers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=binary%20trees" title="binary trees">binary trees</a>, <a href="https://publications.waset.org/abstracts/search?q=MC%2FDC" title=" MC/DC"> MC/DC</a>, <a href="https://publications.waset.org/abstracts/search?q=test%20case%20generation" title=" test case generation"> test case generation</a>, <a href="https://publications.waset.org/abstracts/search?q=nontrivial%20task" title=" nontrivial task"> nontrivial task</a> </p> <a href="https://publications.waset.org/abstracts/29442/practical-methods-for-automatic-mcdc-test-cases-generation-of-boolean-expressions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29442.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">447</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4417</span> Effect of Extraction Methods on the Fatty Acids and Physicochemical Properties of Serendipity Berry Seed Oil</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Olufunmilola%20A.%20Abiodun">Olufunmilola A. Abiodun</a>, <a href="https://publications.waset.org/abstracts/search?q=Adegbola%20O.%20Dauda"> Adegbola O. Dauda</a>, <a href="https://publications.waset.org/abstracts/search?q=Ayobami%20Ojo"> Ayobami Ojo</a>, <a href="https://publications.waset.org/abstracts/search?q=Samson%20A.%20Oyeyinka"> Samson A. Oyeyinka</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Serendipity berry (Dioscoreophyllum cumminsii diel) is a tropical dioecious rainforest vine and native to tropical Africa. The vine grows during the raining season and is used mainly as sweetener. The sweetener in the berry is known as monellin which is sweeter than sucrose. The sweetener is extracted from the fruits and the seed is discarded. The discarded seeds contain bitter principles but had high yield of oil. Serendipity oil was extracted using three methods (N-hexane, expression and expression/n-hexane). Fatty acids and physicochemical properties of the oil obtained were determined. The oil obtained was clear, liquid and have odour similar to hydrocarbon. The percentage oil yield was 38.59, 12.34 and 49.57% for hexane, expression and expression-hexane method respectively. The seed contained high percentage of oil especially using combination of expression and hexane. Low percentage of oil was obtained using expression method. The refractive index values obtained were 1.443, 1.442 and 1.478 for hexane, expression and expression-hexane methods respectively. Peroxide value obtained for expression-hexane was higher than those for hexane and expression. The viscosities of the oil were 125.8, 128.76 and 126.87 cm³/s for hexane, expression and expression-hexane methods respectively which showed that the oil from expression method was more viscous than the other oils. The major fatty acids in serendipity seed oil were oleic acid (62.81%), linoleic acid (22.65%), linolenic (6.11%), palmitic acid (5.67%), stearic acid (2.21%) in decreasing order. Oleic acid which is monounsaturated fatty acid had the highest value. Total unsaturated fatty acids were 91.574, 92.256 and 90.426% for hexane, expression, and expression-hexane respectively. Combination of expression and hexane for extraction of serendipity oil produced high yield of oil. The oil could be refined for food and non-food application. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=serendipity%20seed%20oil" title="serendipity seed oil">serendipity seed oil</a>, <a href="https://publications.waset.org/abstracts/search?q=expression%20method" title=" expression method"> expression method</a>, <a href="https://publications.waset.org/abstracts/search?q=fatty%20acid" title=" fatty acid"> fatty acid</a>, <a href="https://publications.waset.org/abstracts/search?q=hexane" title=" hexane"> hexane</a> </p> <a href="https://publications.waset.org/abstracts/79242/effect-of-extraction-methods-on-the-fatty-acids-and-physicochemical-properties-of-serendipity-berry-seed-oil" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/79242.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">273</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4416</span> Freedom of Expression and Its Restriction in Audiovisual Media</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sevil%20Yildiz">Sevil Yildiz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Audio visual communication is a type of collective expression. Collective expression activity informs the masses, gives direction to opinions and establishes public opinion. Due to these characteristics, audio visual communication must be subjected to special restrictions. This has been stipulated in both the Constitution and the European Human Rights Agreement. This paper aims to review freedom of expression and its restriction in audio visual media. For this purpose, the authorisation of the Radio and Television Supreme Council to impose sanctions as an independent administrative authority empowered to regulate the field of audio visual communication has been reviewed with regard to freedom of expression and its limits. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=audio%20visual%20media" title="audio visual media">audio visual media</a>, <a href="https://publications.waset.org/abstracts/search?q=freedom%20of%20expression" title=" freedom of expression"> freedom of expression</a>, <a href="https://publications.waset.org/abstracts/search?q=its%20limits" title=" its limits"> its limits</a>, <a href="https://publications.waset.org/abstracts/search?q=radio%20and%20television%20supreme%20council" title=" radio and television supreme council"> radio and television supreme council</a> </p> <a href="https://publications.waset.org/abstracts/39325/freedom-of-expression-and-its-restriction-in-audiovisual-media" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39325.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">326</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4415</span> Bcl-2: A Molecule to Detect Oral Cancer and Precancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vandana%20Singh">Vandana Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Subash%20Singh"> Subash Singh </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Oral squamous cell carcinoma is the most common malignant tumor of the oral cavity. Normally the death of cell and the growth are active processes and depend not only on external factors but also on the expression of genes like Bcl-2, which activate and inhibit apoptosis. The term Bcl-2 is an acronym for B-cell lymphoma/ leukemia -2 genes. Objectives: An attempt was made to evaluate Bcl-2 oncoprotein expression in patients with oral precancer and cancer and to assess possible correlation between Bcl-2 oncoprotein expression and clinicopathological features of oral precancer and cancer. Material and Methods: This is a selective prospective clinical and immunohistochemical study. Clinicopathological examination is correlated with immunohistochemical findings. The immunolocalization of Bcl-2 protein is performed using the labeled streptavidin biotin (LSAB) method. To visualize the reaction, 3, 3-diaminobenzidine (DAB) is used. Results: Bcl-2 expression was positive in 11 [36.66 %, low Bcl-2 expression 3 (10.00 %), moderate Bcl-2 expression 7 (23.33 %), and high Bcl-2 expression 1 (3.33 %)] oral cancer cases and in 14 [87.50 %, low expression 8 (50 %), moderate expression 6 (37.50 %)] precancer cases. Conclusion: On the basis of the results of our study we conclude that positive Bcl-2 expression may be an indicator of poor prognosis in oral cancer and precancer. Relevance: It has been reported that there is deregulation of Bcl-2 expression during progression from oral epithelial dysplasia to squamous cell carcinoma. It can be used for revealing progression of epithelial dysplasia to malignancy and as a prognostic marker in oral precancer and cancer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=BcL-2" title="BcL-2">BcL-2</a>, <a href="https://publications.waset.org/abstracts/search?q=immunohistochemistry" title=" immunohistochemistry"> immunohistochemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=oral%20cancer" title=" oral cancer"> oral cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=oral%20precancer" title=" oral precancer"> oral precancer</a> </p> <a href="https://publications.waset.org/abstracts/79418/bcl-2-a-molecule-to-detect-oral-cancer-and-precancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/79418.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">269</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4414</span> Expression of Interferon-Lambda Receptor-(IFN-λRα) in Mononuclear Phagocyte Cells (MPCs) Is Influenced by the Levels of Newly Discovered Type III IFN-λ4 in Vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hashaam%20Akhtar">Hashaam Akhtar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> IFNλR1 and IL10R2 collectively construct a heterodimer, which is an acknowledged functional receptor for all type III interferons (IFNs). Expression of IFNλR1 is highly tissue specific, which can help in making type III IFNs a drug of choice as comparable to its analogue, type I IFNs, for treating hepatitis C in the near future. Although, expression of IFNλR1 also varies with the concentration of type I IFNs, but in this study it was shown that the expression of IFNλR1 varies with the protein titers of IFN-α, IFN-λ3 and the newly discovered IFN-λ4. High dosage of IFN-α reduces the expression of IFNλR1 in HepG2 cells, which can affect the antiviral activity of type III IFNs in vivo. We premeditated an experimental strategy to differentiate monocytes into dendritic cells (DCs), type I and type II macrophages in vitro and quantified the expression of the IFNλR1 by qPCR. The exposure of newly discovered IFN-λ4 to macrophages and DCs also raised the expression of its own receptor, which shows that expression of IFN-λ4 protein in hepatitis C patient may augment type I treatment and help ease off viral titers. The results of this study may contribute in some understanding towards the mechanisms involved in the selective expression of IFNLR1 and exceptionalities associated with the receptor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=IFNLR1" title="IFNLR1">IFNLR1</a>, <a href="https://publications.waset.org/abstracts/search?q=Interferon%20Lambda%204%20%28IFN-%CE%BB4%29" title=" Interferon Lambda 4 (IFN-λ4)"> Interferon Lambda 4 (IFN-λ4)</a>, <a href="https://publications.waset.org/abstracts/search?q=Mononuclear%20Phagocyte%20Cells%20%28MPCs%29" title=" Mononuclear Phagocyte Cells (MPCs)"> Mononuclear Phagocyte Cells (MPCs)</a>, <a href="https://publications.waset.org/abstracts/search?q=expression" title=" expression"> expression</a> </p> <a href="https://publications.waset.org/abstracts/17385/expression-of-interferon-lambda-receptor-ifn-lra-in-mononuclear-phagocyte-cells-mpcs-is-influenced-by-the-levels-of-newly-discovered-type-iii-ifn-l4-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17385.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">385</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4413</span> MicroRNA 200c-3p Regulates Autophagy Mediated Upregulation of Endoplasmic Reticulum Stress in PC-3 Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eun%20Jung%20Sohn">Eun Jung Sohn</a>, <a href="https://publications.waset.org/abstracts/search?q=Hwan%20Tae%20Park"> Hwan Tae Park</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Autophagy is a cellular response to stress or environment on cell survival. Here, we investigated the role of ectopic expression of miR 200c-3p in autophagy. Ectopic expression of miR 200c-3p increased the expression of IRE1alpha, ATF6 and CHOP by western blot and RT-qPCR. Furthermore, the level of microRNA 200c-3p was enhanced by treatment of TG or overexpression of GRP 78. Also, ectopic expression of miR200c-3p increased the LC3 II expression by western blot and RT-qPCR. Also, we found that western blot assay showed that miR200c-3p inhibitor was blocked the starvation–induced LC3II levels. Furthermore, starvation stress increased the level of miR200c-3p in different kinetics. Ectopic expression of miR200c-3p attenuated LC3II expression in IRE1 siRNA transfected PC3 cells. Here, we first demonstrate that miR200c-3p regulates autophagy via ER stress pathway. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Autophagy" title="Autophagy">Autophagy</a>, <a href="https://publications.waset.org/abstracts/search?q=ER%20stress" title=" ER stress"> ER stress</a>, <a href="https://publications.waset.org/abstracts/search?q=LC3II" title=" LC3II"> LC3II</a>, <a href="https://publications.waset.org/abstracts/search?q=miR200c-3p" title=" miR200c-3p"> miR200c-3p</a> </p> <a href="https://publications.waset.org/abstracts/75721/microrna-200c-3p-regulates-autophagy-mediated-upregulation-of-endoplasmic-reticulum-stress-in-pc-3-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75721.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">287</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4412</span> Finding Bicluster on Gene Expression Data of Lymphoma Based on Singular Value Decomposition and Hierarchical Clustering</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alhadi%20Bustaman">Alhadi Bustaman</a>, <a href="https://publications.waset.org/abstracts/search?q=Soeganda%20Formalidin"> Soeganda Formalidin</a>, <a href="https://publications.waset.org/abstracts/search?q=Titin%20Siswantining"> Titin Siswantining</a> </p> <p class="card-text"><strong>Abstract:</strong></p> DNA microarray technology is used to analyze thousand gene expression data simultaneously and a very important task for drug development and test, function annotation, and cancer diagnosis. Various clustering methods have been used for analyzing gene expression data. However, when analyzing very large and heterogeneous collections of gene expression data, conventional clustering methods often cannot produce a satisfactory solution. Biclustering algorithm has been used as an alternative approach to identifying structures from gene expression data. In this paper, we introduce a transform technique based on singular value decomposition to identify normalized matrix of gene expression data followed by Mixed-Clustering algorithm and the Lift algorithm, inspired in the node-deletion and node-addition phases proposed by Cheng and Church based on Agglomerative Hierarchical Clustering (AHC). Experimental study on standard datasets demonstrated the effectiveness of the algorithm in gene expression data. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=agglomerative%20hierarchical%20clustering%20%28AHC%29" title="agglomerative hierarchical clustering (AHC)">agglomerative hierarchical clustering (AHC)</a>, <a href="https://publications.waset.org/abstracts/search?q=biclustering" title=" biclustering"> biclustering</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20expression%20data" title=" gene expression data"> gene expression data</a>, <a href="https://publications.waset.org/abstracts/search?q=lymphoma" title=" lymphoma"> lymphoma</a>, <a href="https://publications.waset.org/abstracts/search?q=singular%20value%20decomposition%20%28SVD%29" title=" singular value decomposition (SVD)"> singular value decomposition (SVD)</a> </p> <a href="https://publications.waset.org/abstracts/72889/finding-bicluster-on-gene-expression-data-of-lymphoma-based-on-singular-value-decomposition-and-hierarchical-clustering" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72889.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">278</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4411</span> Soil Rehabilitation Using Modified Diatomite: Assessing Chemical Properties, Enzymatic Reactions and Heavy Metal Immobilization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Samani.%20Ahmad%20Golchin.%20Hosseinali%20Alikkani.%20Ahmad%20Baybordi">Maryam Samani. Ahmad Golchin. Hosseinali Alikkani. Ahmad Baybordi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Natural diatomite was modified by grinding and acid treatment to increase surface area and to decrease the impurities. Surface area and pore volume of the modified diatomite were 67.45 m² g-1 and 0.105 cm³ g-¹ respectively, and used to immobilize Pb, Zn and Cu in an urban soil. The modified diatomite was added to soil samples at the rates of 2.5, 5, 7.5 and 10% and the samples incubated for 60 days. The addition of modified diatomite increased SSA of the soil. The SSAs of soils with 2.5, 5.0, 7.5 and 10% modified diatomite were 20.82, 22.02, 23.21 and 24.41 m² g-¹ respectively. Increasing the SSAs of the soils by the application of modified diatomite reduced the DTPA extractable concentrations of heavy metals compared with un-amendment control. The concentration of Pb, Zn and Cu were reduced by 91.1%, 82% and 91.1% respectively. Modified diatomite reduced the concentration of Exchangeable and Carbonate bounded species of Pb, Zn and Cu, compared with the control. Also significantly increased the concentration of Fe Mn- OX (Fe-Mn Oxides) and OM (Organic Matter) bound and Res (Residual) fraction. Modified diatomite increased the urease, dehydrogenase and alkaline phosphatase activity by 52%, 57% and 56.6% respectively. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=modified%20diatomite" title="modified diatomite">modified diatomite</a>, <a href="https://publications.waset.org/abstracts/search?q=chemical%20specifications" title=" chemical specifications"> chemical specifications</a>, <a href="https://publications.waset.org/abstracts/search?q=specific%20surface%20area" title=" specific surface area"> specific surface area</a>, <a href="https://publications.waset.org/abstracts/search?q=enzyme%20activity" title=" enzyme activity"> enzyme activity</a>, <a href="https://publications.waset.org/abstracts/search?q=immobilization" title=" immobilization"> immobilization</a>, <a href="https://publications.waset.org/abstracts/search?q=heavy%20metal" title=" heavy metal"> heavy metal</a>, <a href="https://publications.waset.org/abstracts/search?q=soil%20remediation" title=" soil remediation"> soil remediation</a> </p> <a href="https://publications.waset.org/abstracts/180554/soil-rehabilitation-using-modified-diatomite-assessing-chemical-properties-enzymatic-reactions-and-heavy-metal-immobilization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/180554.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">63</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4410</span> Freedom with Limitations: The Nature of Free Expression in the European Case-Law</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Laszlo%20Vari">Laszlo Vari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the digital age, the spread of the mobile world and the nature of the cyberspace, offers many new opportunities for the prevalence of the fundamental right to free expression, and therefore, for free speech and freedom of the press; however, these new information communication technologies carry many new challenges. Defamation, censorship, fake news, misleading information, hate speech, breach of copyright etc., are only some of the violations, all of which can be derived from the harmful exercise of freedom of expression, all which become more salient in the internet. Here raises the question: how can we eliminate these problems, and practice our fundamental freedom rightfully? To answer this question, we should understand the elements and the characteristic of the nature of freedom of expression, and the role of the actors whose duties and responsibilities are crucial in the prevalence of this fundamental freedom. To achieve this goal, this paper will explore the European practice to understand instructions found in the case-law of the European Court of Human rights for the rightful exercise of freedom of expression. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=collision%20of%20rights" title="collision of rights">collision of rights</a>, <a href="https://publications.waset.org/abstracts/search?q=European%20case-law" title=" European case-law"> European case-law</a>, <a href="https://publications.waset.org/abstracts/search?q=freedom%20opinion%20and%20expression" title=" freedom opinion and expression"> freedom opinion and expression</a>, <a href="https://publications.waset.org/abstracts/search?q=media%20law" title=" media law"> media law</a>, <a href="https://publications.waset.org/abstracts/search?q=freedom%20of%20information" title=" freedom of information"> freedom of information</a>, <a href="https://publications.waset.org/abstracts/search?q=online%20expression" title=" online expression"> online expression</a> </p> <a href="https://publications.waset.org/abstracts/104514/freedom-with-limitations-the-nature-of-free-expression-in-the-european-case-law" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104514.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4409</span> Recognition of New Biomarkers in the Epigenetic Pathway of Breast Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fatemeh%20Zeinali%20Sehrig">Fatemeh Zeinali Sehrig</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study aimed to evaluate the expression of miR-299-3p, DNMT1, DNMT3A, and DNMT3B in breast cancer samples and investigate their diagnostic significance. Using the GSE40525 and GSE45666, the miR-299-3p expression level was studied in breast cancer tissues. Also, the expression levels of DNMT1, DNMT3A, and DNMT3B were investigated by analyzing GSE61725, GSE86374, and GSE37751 datasets. The target genes were studied in terms of biological processes of molecular functions and cellular components. Consistent with the in silico results, miR-299-3p expression was substantially decreased in breast cancer tissues, and the expression levels of DNMT1, DNMT3A, and DNMT3B were considerably upregulated in breast cancer samples. It was found that the expression levels of miR-299-3p and DNMT1, DNMT3A, and DNMT3B could be valuable diagnostic tools for detecting breast cancer. Also, miR-299-3p downregulation may play a role in DNMT1, DNMT3A, and DNMT3B upregulation in breast cancer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title="breast cancer">breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=miR-299-3p" title=" miR-299-3p"> miR-299-3p</a>, <a href="https://publications.waset.org/abstracts/search?q=DNMTs" title=" DNMTs"> DNMTs</a>, <a href="https://publications.waset.org/abstracts/search?q=GEO%20database" title=" GEO database"> GEO database</a> </p> <a href="https://publications.waset.org/abstracts/188484/recognition-of-new-biomarkers-in-the-epigenetic-pathway-of-breast-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188484.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">37</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4408</span> Real Time PCR Analysis of microRNA Expression in Oral Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Karl%20Kingsley">Karl Kingsley</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Many mechanisms are involved in the control of cellular differentiation and growth, which are often dysregulated in many cancers. Many distinct pathways are involved in these mechanisms of control, including deoxyribonuclease (DNA) methyltransferase and histone deacetylase (HDAC) activation that controls both genetic and epigenetic modifications and micro ribonucleic acid (RNA) expression. Less is known about the expression of DNA methyltransferase (DNMT) and HDAC in oral cancers and the effect on microRNA expression. The primary objective of this study was to evaluate the expression of DNMT and HDAC family members in oral cancer and the concomitant expression of cancer-associated microRNAs. Using commercially available oral cancers, including squamous cell carcinoma (SCC)-4, SCC-9, SCC-15, and SCC-25, RNA was extracted and screened for DNMT, HDAC, and microRNA expression using highly-specific primers and quantitative polymerase chain reaction (qPCR). These data revealed low or absent expression of DNMT-1, which is associated with cellular differentiation but increased expression of DNMT-3a and DNMT-3b in all SCC cell lines compared with normal non-cancerous cell controls. In addition, no expression of HDAC1 and HDAC2 expression was found among the normal, non-cancerous cells but was highly expressed in each of the SCC cell lines examined. Differential expression of oncogenic and cancer-associated microRNAs was also observed among the SCC cell lines, including miR-21, miR-133, miR-149, miR-155, miR-365, and miR-720. These findings also appeared to vary according to observed growth rates among these cells. These data may be the first to demonstrate the expression and association between HDAC and DNMT3 family members among oral cancers. In addition, the differential expression of these epigenetic modifiers may be associated with the expression of specific microRNAs in these cancers, which have not previously been observed to the best of the author's knowledge. In addition, some associations and relationships may exist between the expression of these biomarkers and the rates of growth and proliferation, which may suggest that these expression patterns might represent potentially useful biomarkers to determine tumor aggressiveness and other phenotypic behaviors among oral cancers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=oral%20cancer" title="oral cancer">oral cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20methyltransferase" title=" DNA methyltransferase"> DNA methyltransferase</a>, <a href="https://publications.waset.org/abstracts/search?q=histone%20deacetylase" title=" histone deacetylase"> histone deacetylase</a>, <a href="https://publications.waset.org/abstracts/search?q=microRNA" title=" microRNA"> microRNA</a> </p> <a href="https://publications.waset.org/abstracts/114439/real-time-pcr-analysis-of-microrna-expression-in-oral-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/114439.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4407</span> Autonomy and Other Variables Related to the Expression of Love among Saudi Couples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reshaa%20Alruwaili">Reshaa Alruwaili</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The primary aim of this study was to examine the hypothesis presented by Self Determination theory which suggests that autonomy impacts positively the expression of love. Other hypotheses were also examined which suggest that other variables explain the expression of love, including: dyadic adjustment (dyadic consensus, dyadic satisfaction and dyadic cohesion), couple satisfaction, age, gender, the length of marriage, number of children and attachment styles. The participants were Saudi couples, which provided the opportunity to consider the influence of Saudi culture on the expression of love. A questionnaire was employed to obtain measures of all the relevant variables, including a measure of expression of love that was built from 27 items, constituting verbal, physical and caring features, and a measure of autonomy based on three features: authorship, interest-taking and susceptibility. Data were collected from both members of 34 Saudi couples. Descriptive analysis of both expression of love and autonomy was conducted. Correlation and regression were used to assess the relationships between expression of love and autonomy and other variables. Results indicated that Saudi couples who most often express their love tend to be more than somewhat autonomous. Not much difference was found between husbands and wives in expressing love, although wives were slightly more autonomous than husbands. Expression of love was enhanced by the autonomy of the participants to a greater extent when dyadic satisfaction was controlled, since the latter was negatively correlated with autonomy and had no effect on the expression of love. Basic psychological needs, dyadic consensus and dismissive-avoidant attachment improve the expression of love, while it is decreased by the number of children. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=autonomy" title="autonomy">autonomy</a>, <a href="https://publications.waset.org/abstracts/search?q=determination%20theory" title=" determination theory"> determination theory</a>, <a href="https://publications.waset.org/abstracts/search?q=expression%20of%20love" title=" expression of love"> expression of love</a>, <a href="https://publications.waset.org/abstracts/search?q=dyadic%20adjustment" title=" dyadic adjustment "> dyadic adjustment </a> </p> <a href="https://publications.waset.org/abstracts/73995/autonomy-and-other-variables-related-to-the-expression-of-love-among-saudi-couples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/73995.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">232</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4406</span> Cellular Degradation Activity is Activated by Ambient Temperature Reduction in an Annual Fish (Nothobranchius rachovii)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cheng-Yen%20Lu">Cheng-Yen Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Chin-Yuan%20Hsu"> Chin-Yuan Hsu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ambient temperature reduction (ATR) can extend the lifespan of an annual fish (Nothobranchius rachovii), but the underlying mechanism is unknown. In this study, the expression, concentration, and activity of cellular-degraded molecules were evaluated in the muscle of N. rachovii reared under high (30 °C), moderate (25 °C), and low (20 °C) ambient temperatures by biochemical techniques. The results showed that (i) the activity of the 20S proteasome, the expression of microtubule-associated protein 1 light chain 3-II (LC3-II), the expression of lysosome-associated membrane protein type 2a (Lamp 2a), and lysosome activity increased with ATR; (ii) the expression of the 70 kD heat shock cognate protein (Hsc 70) decreased with ATR; (iii) the expression of the 20S proteasome, the expression of lysosome-associated membrane protein type 1 (Lamp 1), the expression of molecular target of rapamycin (mTOR), the expression of phosphorylated mTOR (p-mTOR), and the p-mTOR/mTOR ratio did not change with ATR. These findings indicated that ATR activated the activity of proteasome, macroautophagy, and chaperone-mediated autophagy. Taken together these data reveal that ATR likely activates cellular degradation activity to extend the lifespan of N. rachovii. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ambient%20temperature%20reduction" title="ambient temperature reduction">ambient temperature reduction</a>, <a href="https://publications.waset.org/abstracts/search?q=autophagy" title=" autophagy"> autophagy</a>, <a href="https://publications.waset.org/abstracts/search?q=degradation%20activity" title=" degradation activity"> degradation activity</a>, <a href="https://publications.waset.org/abstracts/search?q=lifespan" title=" lifespan"> lifespan</a>, <a href="https://publications.waset.org/abstracts/search?q=proteasome" title=" proteasome"> proteasome</a> </p> <a href="https://publications.waset.org/abstracts/23211/cellular-degradation-activity-is-activated-by-ambient-temperature-reduction-in-an-annual-fish-nothobranchius-rachovii" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23211.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">459</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4405</span> Biophysically Motivated Phylogenies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Catherine%20Felce">Catherine Felce</a>, <a href="https://publications.waset.org/abstracts/search?q=Lior%20Pachter"> Lior Pachter</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Current methods for building phylogenetic trees from gene expression data consider mean expression levels. With single-cell technologies, we can leverage more information about cell dynamics by considering the entire distribution of gene expression across cells. Using biophysical modeling, we propose a method for constructing phylogenetic trees from scRNA-seq data, building on Felsenstein's method of continuous characters. This method can highlight genes whose level of expression may be unchanged between species, but whose rates of transcription/decay may have evolved over time. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phylogenetics" title="phylogenetics">phylogenetics</a>, <a href="https://publications.waset.org/abstracts/search?q=single-cell" title=" single-cell"> single-cell</a>, <a href="https://publications.waset.org/abstracts/search?q=biophysical%20modeling" title=" biophysical modeling"> biophysical modeling</a>, <a href="https://publications.waset.org/abstracts/search?q=transcription" title=" transcription"> transcription</a> </p> <a href="https://publications.waset.org/abstracts/186016/biophysically-motivated-phylogenies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186016.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">50</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4404</span> Comparison of Silica-Filled Rubber Compound Prepared from Unmodified and Modified Silica</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thirawudh%20Pongprayoon">Thirawudh Pongprayoon</a>, <a href="https://publications.waset.org/abstracts/search?q=Watcharin%20Rassamee"> Watcharin Rassamee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Silica-filled natural rubber compounds were prepared from unmodified and surface-modified silica. The modified silica was coated by ultrathin film of polyisoprene by admicellar polymerization. FTIR and SEM were applied to characterize the modified silica. The cure, mechanic, and dynamics properties were investigated with the comparison of the compounds. Cure characterization of modified silica rubber compound was shorter than that of unmodified silica compound. Strength and abrasion resistance of modified silica compound were better than those of unmodified silica rubber compound. Wet grip and rolling resistance analyzed by DMA from tanδ at 0°C and 60°C using 5 Hz were also better than those of unmodified silica rubber compound. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=silica" title="silica">silica</a>, <a href="https://publications.waset.org/abstracts/search?q=admicellar%20polymerization" title=" admicellar polymerization"> admicellar polymerization</a>, <a href="https://publications.waset.org/abstracts/search?q=rubber%20compounds" title=" rubber compounds"> rubber compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=mechanical%20properties" title=" mechanical properties"> mechanical properties</a>, <a href="https://publications.waset.org/abstracts/search?q=dynamic%20properties" title=" dynamic properties"> dynamic properties</a> </p> <a href="https://publications.waset.org/abstracts/12331/comparison-of-silica-filled-rubber-compound-prepared-from-unmodified-and-modified-silica" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12331.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">350</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4403</span> Using Gene Expression Programming in Learning Process of Rough Neural Networks</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sanaa%20Rashed%20Abdallah">Sanaa Rashed Abdallah</a>, <a href="https://publications.waset.org/abstracts/search?q=Yasser%20F.%20Hassan"> Yasser F. Hassan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The paper will introduce an approach where a rough sets, gene expression programming and rough neural networks are used cooperatively for learning and classification support. The Objective of gene expression programming rough neural networks (GEP-RNN) approach is to obtain new classified data with minimum error in training and testing process. Starting point of gene expression programming rough neural networks (GEP-RNN) approach is an information system and the output from this approach is a structure of rough neural networks which is including the weights and thresholds with minimum classification error. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rough%20sets" title="rough sets">rough sets</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20expression%20programming" title=" gene expression programming"> gene expression programming</a>, <a href="https://publications.waset.org/abstracts/search?q=rough%20neural%20networks" title=" rough neural networks"> rough neural networks</a>, <a href="https://publications.waset.org/abstracts/search?q=classification" title=" classification"> classification</a> </p> <a href="https://publications.waset.org/abstracts/41805/using-gene-expression-programming-in-learning-process-of-rough-neural-networks" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41805.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">383</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4402</span> Incorporation of Noncanonical Amino Acids into Hard-to-Express Antibody Fragments: Expression and Characterization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hana%20Hanaee-Ahvaz">Hana Hanaee-Ahvaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Monika%20Cserjan-Puschmann"> Monika Cserjan-Puschmann</a>, <a href="https://publications.waset.org/abstracts/search?q=Christopher%20Tauer"> Christopher Tauer</a>, <a href="https://publications.waset.org/abstracts/search?q=Gerald%20Striedner"> Gerald Striedner</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Incorporation of noncanonical amino acids (ncAA) into proteins has become an interesting topic as proteins featured with ncAAs offer a wide range of different applications. Nowadays, technologies and systems exist that allow for the site-specific introduction of ncAAs in vivo, but the efficient production of proteins modified this way is still a big challenge. This is especially true for 'hard-to-express' proteins where low yields are encountered even with the native sequence. In this study, site-specific incorporation of azido-ethoxy-carbonyl-Lysin (azk) into an anti-tumor-necrosis-factor-α-Fab (FTN2) was investigated. According to well-established parameters, possible site positions for ncAA incorporation were determined, and corresponding FTN2 genes were constructed. Each of the modified FTN2 variants has one amber codon for azk incorporated either in its heavy or light chain. The expression level for all variants produced was determined by ELISA, and all azk variants could be produced with a satisfactory yield in the range of 50-70% of the original FTN2 variant. In terms of expression yield, neither the azk incorporation position nor the subunit modified (heavy or light chain) had a significant effect. We confirmed correct protein processing and azk incorporation by mass spectrometry analysis, and antigen-antibody interaction was determined by surface plasmon resonance analysis. The next step is to characterize the effect of azk incorporation on protein stability and aggregation tendency via differential scanning calorimetry and light scattering, respectively. In summary, the incorporation of ncAA into our Fab candidate FTN2 worked better than expected. The quantities produced allowed a detailed characterization of the variants in terms of their properties, and we can now turn our attention to potential applications. By using click chemistry, we can equip the Fabs with additional functionalities and make them suitable for a wide range of applications. We will now use this option in a first approach and develop an assay that will allow us to follow the degradation of the recombinant target protein in vivo. Special focus will be laid on the proteolytic activity in the periplasm and how it is influenced by cultivation/induction conditions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=degradation" title="degradation">degradation</a>, <a href="https://publications.waset.org/abstracts/search?q=FTN2" title=" FTN2"> FTN2</a>, <a href="https://publications.waset.org/abstracts/search?q=hard-to-express%20protein" title=" hard-to-express protein"> hard-to-express protein</a>, <a href="https://publications.waset.org/abstracts/search?q=non-canonical%20amino%20acids" title=" non-canonical amino acids"> non-canonical amino acids</a> </p> <a href="https://publications.waset.org/abstracts/138030/incorporation-of-noncanonical-amino-acids-into-hard-to-express-antibody-fragments-expression-and-characterization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/138030.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">232</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4401</span> A Novel PfkB Gene Cloning and Characterization for Expression in Potato Plants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arfan%20Ali">Arfan Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Idrees%20Ahmad%20Nasir"> Idrees Ahmad Nasir</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Potato (Solanum tuberosum) is an important cash crop and popular vegetable in Pakistan and throughout the world. Cold storage of potatoes accelerates the conversion of starch into reduced sugars (glucose and fructose). This process causes dry mass and bitter taste in the potatoes that are not acceptable to end consumers. In the current study, the phosphofructokinase B gene was cloned into the pET-30 vector for protein expression and the pCambia-1301 vector for plant expression. Amplification of a 930bp product from an E. coli strain determined the successful isolation of the phosphofructokinase B gene. Restriction digestion using NcoI and BglII along with the amplification of the 930bp product using gene specific primers confirmed the successful cloning of the PfkB gene in both vectors. The protein was expressed as a His-PfkB fusion protein. Western blot analysis confirmed the presence of the 35 Kda PfkB protein when hybridized with anti-His antibodies. The construct Fani-01 was evaluated transiently using a histochemical gus assay. The appearance of blue color in the agroinfiltrated area of potato leaves confirmed the successful expression of construct Fani-01. Further, the area displaying gus expression was evaluated for PfkB expression using ELISA. Moreover, PfkB gene expression evaluated through transient expression determined successful gene expression and highlighted its potential utilization for stable expression in potato to reduce sweetening due to long-term storage. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=potato" title="potato">potato</a>, <a href="https://publications.waset.org/abstracts/search?q=Solanum%20tuberosum" title=" Solanum tuberosum"> Solanum tuberosum</a>, <a href="https://publications.waset.org/abstracts/search?q=transformation" title=" transformation"> transformation</a>, <a href="https://publications.waset.org/abstracts/search?q=PfkB" title=" PfkB"> PfkB</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-sweetening" title=" anti-sweetening "> anti-sweetening </a> </p> <a href="https://publications.waset.org/abstracts/24921/a-novel-pfkb-gene-cloning-and-characterization-for-expression-in-potato-plants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24921.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">472</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4400</span> Difference in the Expression of CIRBP, RBM3 and HSP70 in the Myocardium and Cerebellum after Death by Hypothermi a and Carbon Monoxide Poisoning</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Satoshi%20Furukawa">Satoshi Furukawa</a>, <a href="https://publications.waset.org/abstracts/search?q=Satomu%20Morita"> Satomu Morita</a>, <a href="https://publications.waset.org/abstracts/search?q=Lisa%20Wingenfeld"> Lisa Wingenfeld</a>, <a href="https://publications.waset.org/abstracts/search?q=Katsuji%20Nishi"> Katsuji Nishi</a>, <a href="https://publications.waset.org/abstracts/search?q=Masahito%20Hitosugi"> Masahito Hitosugi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We studied the expression of hypoxia-related antigens (e.g., cold-inducible antigens and apoptotic antigens) in the myocardium and the cerebellumthat were obtained from individuals after death by carbon monoxide or hypothermia. The immunohistochemistry results revealed that expression of cold-inducible RNA binding protein (CIRBP) and RNA-binding protein 3 (RBM3) may be associated with hpyothermic and the hypoxic conditions. The expression of CIRBP and RBM3 in the myocardium was different from their expression in the cerebellum, especially in the Purkinje cells. The results indicate that agonal duration influences antigen expression. In the hypothermic condition, the myocardium uses more ATP since the force of the excitation-contraction coupling of the myocardium increases by more than 400% when the experimental temperature is reduced from 35°C to 25°C. The results obtained in this study indicate that physicians should pay attention to the myocardium when cooling the patient’s body to protect the brain. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=carbon%20monoxide%20death" title="carbon monoxide death">carbon monoxide death</a>, <a href="https://publications.waset.org/abstracts/search?q=cerebellum" title=" cerebellum"> cerebellum</a>, <a href="https://publications.waset.org/abstracts/search?q=CIRBP" title=" CIRBP"> CIRBP</a>, <a href="https://publications.waset.org/abstracts/search?q=hypothermic%20death" title=" hypothermic death"> hypothermic death</a>, <a href="https://publications.waset.org/abstracts/search?q=myocardium" title=" myocardium"> myocardium</a>, <a href="https://publications.waset.org/abstracts/search?q=RBM3" title=" RBM3"> RBM3</a> </p> <a href="https://publications.waset.org/abstracts/13000/difference-in-the-expression-of-cirbp-rbm3-and-hsp70-in-the-myocardium-and-cerebellum-after-death-by-hypothermi-a-and-carbon-monoxide-poisoning" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13000.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">363</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4399</span> Innate Immune Expression in Heterophils in Response to LPS</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rohita%20Gupta">Rohita Gupta</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20S.%20Brah"> G. S. Brah</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Verma"> R. Verma</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20S.%20Mukhopadhayay"> C. S. Mukhopadhayay</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Although chicken strains show differences in susceptibility to a number of diseases, the underlying immunological basis is yet to be elucidated. In the present study, heterophils were subjected to LPS stimulation and total RNA extraction, further differential gene expression was studied in broiler, layer and indigenous Aseel strain by Real Time RT-PCR at different time periods before and after induction. The expression of the 14 AvBDs and chTLR 1, 2, 3, 4, 5, 7, 15 and 21 was detectable in heterophils. The expression level of most of the AvBDs significantly increased (P<0.05) 3 hours post in vitro lipopolysaccharide challenge. Higher expression level and stronger activation of most AvBDs, NFkB-1 and IRF-3 in heterophils was observed with the stimulation of LPS in layer compared to broiler, and in Aseel compared to both layer and broiler. This investigation will allow more refined interpretation of immuno-genetic basis of the variable disease resistance/susceptibility in divergent stock of chicken including indigenous breed. Moreover, this study will be helpful in formulation of strategy for isolation of antimicrobial peptides from heterophils. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=differential%20expression" title="differential expression">differential expression</a>, <a href="https://publications.waset.org/abstracts/search?q=heterophils" title=" heterophils"> heterophils</a>, <a href="https://publications.waset.org/abstracts/search?q=cytokines" title=" cytokines"> cytokines</a>, <a href="https://publications.waset.org/abstracts/search?q=defensin" title=" defensin"> defensin</a>, <a href="https://publications.waset.org/abstracts/search?q=TLR" title=" TLR"> TLR</a> </p> <a href="https://publications.waset.org/abstracts/10174/innate-immune-expression-in-heterophils-in-response-to-lps" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10174.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">497</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4398</span> Modified Preputial Urethrostomy as a Salvage Procedure in Four Dogs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Giansetto">Thomas Giansetto</a>, <a href="https://publications.waset.org/abstracts/search?q=Olivier%20Broux"> Olivier Broux</a>, <a href="https://publications.waset.org/abstracts/search?q=G%C3%A9raldine%20Bolen"> Géraldine Bolen</a>, <a href="https://publications.waset.org/abstracts/search?q=St%C3%A9phanie%20Claeys"> Stéphanie Claeys</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: To describe a modified preputial urethrostomy in four dogs. Study design: Short case series. Animals: Four male dogs were treated by the modified preputial urethrostomy, three because of urethral stenosis, and one because of severe complications following perineal urethrostomy. Methods: Four dogs were presented for dysuria and urinary obstruction. secondary to urethral stenosis or tear. Obstruction was treated with a modified preputial urethrostomy. Results: Four dogs had resolution of dysuria with a modified preputial urethrostomy without penile amputation. None of the dogs presented signs of dysuria, urinary tract infection, or dermatitis postoperatively. Two dogs showed signs of urinary incontinence 15 days and one month postoperatively. Conclusion: The modified preputial urethrostomy resulted in a favorable prognosis and may be an alternative to prepubic urethrostomy in male dogs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=urethrostomy" title="urethrostomy">urethrostomy</a>, <a href="https://publications.waset.org/abstracts/search?q=preputial" title=" preputial"> preputial</a>, <a href="https://publications.waset.org/abstracts/search?q=technique" title=" technique"> technique</a>, <a href="https://publications.waset.org/abstracts/search?q=urogenital" title=" urogenital"> urogenital</a> </p> <a href="https://publications.waset.org/abstracts/149472/modified-preputial-urethrostomy-as-a-salvage-procedure-in-four-dogs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/149472.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">168</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4397</span> Using Support Vector Machines for Measuring Democracy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tommy%20Krieger">Tommy Krieger</a>, <a href="https://publications.waset.org/abstracts/search?q=Klaus%20Gruendler"> Klaus Gruendler </a> </p> <p class="card-text"><strong>Abstract:</strong></p> We present a novel approach for measuring democracy, which enables a very detailed and sensitive index. This method is based on Support Vector Machines, a mathematical algorithm for pattern recognition. Our implementation evaluates 188 countries in the period between 1981 and 2011. The Support Vector Machines Democracy Index (SVMDI) is continuously on the 0-1-Interval and robust to variations in the numerical process parameters. The algorithm introduced here can be used for every concept of democracy without additional adjustments, and due to its flexibility it is also a valuable tool for comparison studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=democracy" title="democracy">democracy</a>, <a href="https://publications.waset.org/abstracts/search?q=democracy%20index" title=" democracy index"> democracy index</a>, <a href="https://publications.waset.org/abstracts/search?q=machine%20learning" title=" machine learning"> machine learning</a>, <a href="https://publications.waset.org/abstracts/search?q=support%20vector%20machines" title=" support vector machines"> support vector machines</a> </p> <a href="https://publications.waset.org/abstracts/31697/using-support-vector-machines-for-measuring-democracy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31697.pdf" target="_blank" class="btn btn-primary 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