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PBS-3 | PBS Biotech | PBS Biotech

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class="margin-bottom product-page-name">PBS-3 Vertical-Wheel Bioreactor </h2><h3 class="margin-bottom margin-custom2">The new standard of single-use bioreactor for cell therapy applications </h3><p class="text-color-blue margin-bottom margin-custom1">The PBS-3 Vertical-Wheel bioreactor is designed to take your cell culture process to the next level. Process development and optimization started in PBS-Minis can be scaled up into this self-contained bioreactor with customizable control of process parameters.</p><a href="#quote-button-stop" class="button w-button">Request Quote</a></div></div><div class="product-highlights-wrapper"><h3 class="heading-22">Highlights:</h3><div class="rich-text-block-2 w-richtext"><ul role="list"><li>Single-use vessels and software qualified for use in clinical trials, including first-in-human studies</li><li>Touchscreen interface for intuitive process customization, control, and reporting</li><li>Configurations available for use with single-use or reusable DO and pH sensors</li><li>Installation and operations qualification protocols available for sale</li></ul></div></div></div></div></section><section class="section-catalague"><div class="page-padding"><div class="conferences-container w-container"><div class="catalague-component"><h2 class="product-resources-header">Product Catalogue</h2><div class="rich-text w-richtext"><div class="w-embed"><table id="mini"> <tr> <th>Product Type</th> <th>Product Name</th> <th>Product Number</th> </tr> <tr> <td rowspan="4">Bioreactors</td> <td>PBS-3 Vertical-Wheel Bioreactor (120 V) </td> <td> IA-3-B-501 </td> </tr> <tr> <td> PBS-3 Vertical-Wheel Bioreactor (240 V) </td> <td> IA-3-B-502</td> </tr> <tr> <td> PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors (120 V) </td> <td> IA-3-B-701</td> </tr> <tr> <td> PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors (240 V) </td> <td>IA-3-B-702</td> </tr> <tr> <td rowspan="4">Single-Use Vessels</td> <td>PBS-3 Vertical-Wheel Single-Use Vessel (Floored Wheel) <td>FA-3-D-504 </td> <tr><td> PBS-3 Vertical-Wheel Single-Use Vessel (Floorless Wheel) </td><td>FA-3-D-506 </td> </tr><tr><td> PBS-3 Vertical-Wheel Single-Use Vessel, Single-Use Sensors (Floored Wheel) </td> <td>FA-3-D-704-L </td> </tr><tr> <td> PBS-3 Vertical-Wheel Single-Use Vessel, Single-Use Sensors (Floorless Wheel) </td> <td>FA-3-D-706-L </td> </tr> <tr> <td rowspan="8">Accessories</td> <td>PBS-3 Reusable Thermal Well </td><td>IA-3-BA-006 </td> <tr><td> PBS-3 Dip Tube Assembly </td><td>IA-3-BA-007 </td> </tr> <tr><td> PBS Autoclavable Pouch, 4" x 15" (pack of 2) </td> <td>IA-UNI-DA-003 </td> </tr> <tr><td> PBS Single-Syringe Sampling Line, 20mL </td><td> IA-UNI-DA-006 </td> </tr><tr><td> PBS Autoclavable Pouch, 8" x 18" (pack of 2) </td><td>IA-UNI-DA-007 </td> </tr> <tr><td> Reusable DO Sensor, 12mm OD x 220mm L with Right-Angle </td> <td>IC00468 </td> </tr> <tr><td> Reusable pH Sensor, 12mm OD x 225mm L </td> <td>IC00469 </td> </tr> <tr><td> 60 Micron Plastic (PE) Retention Filters (pack of 5) </td> <td>IC01036 </td> </tr> <tr> <td rowspan="1"> Software </td> <td> PBS-3 Vertical-Wheel Bioreactor Software 3.0 (21 CFR Part 11 cGMP Upgrade) </td> <td>IA-3-SW-001 </td> </tr> <tr> <td rowspan="2">Qualification Packages</td> <td> PBS-3 Vertical-Wheel Bioreactor IQ/OQ Protocols </td> <td>IA-3-BQ-004 </td> <tr> <td> PBS-3 Vertical-Wheel Bioreactor IQ/OQ Configuration Sheet </td> <td>IA-3-BQ-005 </td> </tr> </table> <style> #mini { font-family: Karla; /* border: none; */ width: 100%; } td{ font-family: Karla; font-size: 16px; font-weight: 700; line-height: 24px; letter-spacing: 0px; text-align: left; } #mini td, #mini th { /* border: 1px solid #ddd; */ padding: 8px; } #mini th { padding-top: 13px; padding-bottom: 12px; text-align: center; background-color: #233B5C; color: white; border: 1px solid #C8CCD8; border-left: none; } table, th, td { border: 1px solid #C8CCD8; border-collapse: collapse; } td { border: 1px solid #C8CCD8; } #mini td { background-color: White; } </style></div></div></div></div></div></section><section class="section-technicaal"><div class="page-padding"><div class="conferences-container w-container"><div class="technical-specs-wrapper"><h3 class="tech-spec-title margin-small">Technical Specifications:</h3><img alt="" loading="lazy" width="699" src="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63c1bb3795309bee306fa9b3_PBS-3%20spec%20table.jpg" sizes="100vw" srcset="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63c1bb3795309bee306fa9b3_PBS-3%20spec%20table-p-500.jpg 500w, https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63c1bb3795309bee306fa9b3_PBS-3%20spec%20table.jpg 588w" class="spec-table-image"/></div></div></div></section><div class="section-library"><div class="page-padding"><div class="conferences-container w-container"><div class="library-component"><div class="case-study-component"><h2 class="product-resources-header">Case Studies</h2><div class="case-study-cms w-dyn-list"><div role="list" class="case-study-cms-list w-dyn-items"><div role="listitem" class="w-dyn-item"><div class="case-study-wrap"><div class="case-study-text-wrapper"><div class="case-study-text">Defined Biomatrices Accelerate MSC Manufacturing Across Scales</div></div><a href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/6434a6a639b63b4b7a7ac231_2023%20Defined%20Biomatrices%20Accelerate%20MSC%20Manufacturing%20Across%20Scales.pdf" target="_blank" class="product-link-wrapper w-inline-block"><div class="heading-small text-color-yellow hover">Download</div></a></div></div><div role="listitem" class="w-dyn-item"><div class="case-study-wrap"><div class="case-study-text-wrapper"><div class="case-study-text">Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High-Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors</div></div><a href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63b68833b19e11c7b880dca7_2021%20Borys%20et%20al.%20-%20Overcoming%20Bioprocess%20Bottlenecks%20in%20Large-Scale%20Expansion.pdf" target="_blank" class="product-link-wrapper w-inline-block"><div class="heading-small text-color-yellow hover">Download</div></a></div></div><div role="listitem" class="w-dyn-item"><div class="case-study-wrap"><div class="case-study-text-wrapper"><div class="case-study-text">Initial Power Measurements for a Family of Novel Bioreactors</div></div><a href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63b684a592188ebf9a76f829_2022%20Croughan%20et%20al.%20-%20Initial%20Power%20Measurements%20for%20a%20Family%20of%20Novel%20Bioreactors.pdf" target="_blank" class="product-link-wrapper w-inline-block"><div class="heading-small text-color-yellow hover">Download</div></a></div></div></div></div></div><div class="resources-component"><div class="resources-wrapper"><h2 class="mini-resources-header">User Resources</h2></div><div class="resources-cms-wrapper w-dyn-list"><div role="list" class="resources-cms-list w-dyn-items"><div role="listitem" class="w-dyn-item"><div class="resource-wrap"><div class="case-study-text-wrapper"><div class="resouces-list-text">PBS-3 Single-Use Bioreactor User Manual (Single-Use Sensors, v4 Software) [P/Ns: IA-3-B-711 &amp; -712]</div></div><a href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/660c1858a87bd3c38ebc587f_IC03946%20Rev%20A_digital.pdf" target="_blank" class="product-link-wrapper w-inline-block"><div class="heading-small text-color-yellow hover">Download</div></a></div></div><div role="listitem" class="w-dyn-item"><div class="resource-wrap"><div class="case-study-text-wrapper"><div class="resouces-list-text">PBS-3 Single-Use Bioreactor User Manual (Reusable Sensors, v4 Software) [P/Ns: IA-3-B-511 &amp; -512]</div></div><a href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/660ba621707daf20c0c112ef_IC03945%20Rev%20A_digital.pdf" target="_blank" class="product-link-wrapper w-inline-block"><div class="heading-small text-color-yellow hover">Download</div></a></div></div><div role="listitem" class="w-dyn-item"><div class="resource-wrap"><div class="case-study-text-wrapper"><div class="resouces-list-text">PBS-3 Datasheet</div></div><a href="https://cdn.prod.website-files.com/62cc627d7fe730df74484ebd/63c9e3ee8032c7a5b43f7e40_3L%20Datasheet_print%20v.221007.pdf" target="_blank" class="product-link-wrapper w-inline-block"><div class="heading-small text-color-yellow hover">Download</div></a></div></div></div></div></div></div></div></div></div><div class="section-library"><div class="page-padding"><div class="conferences-container w-container"><div class="library-component"><div class="products-faq-component"><div class="faq-content-wrapper"><h2 class="product-resources-header margin-bottom margin-large">FAQ</h2><div class="w-dyn-list"><div role="list" class="w-dyn-items"><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">What is the role of the 3L Vertical-Wheel bioreactor in process scale up?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">Successful transition of a small scale cell culture process designed in PBS Minis into the 3L bioreactor represents the first step in achieving true scalability of manufacturing therapeutic cells. The 3L can meet production needs for early stage clinical studies and also serves as stepping stone for continued scale up into the 15L bioreactor.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">What is the difference between floored and floorless Vertical-Wheel impellers and what kind of cell culture processes are each suited for?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">The floored version has more panels around the circumference of the Vertical-Wheel that create cup-like sections and is better suited for cells grown on microcarriers or as single cells. The floorless version is nearly identical but with less panels and allows more fluid flow towards the axle, and is better suited for cell aggregate applications. Both versions have nearly identical hydrodynamic performance.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">Are single-use dissolved oxygen and pH sensors available for use with all Vertical-Wheel bioreactors?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">The 3L, 15L, and 80L bioreactors are available in one version that accommodates single-use sensors and another version that is designed for reusable sensors (no cross-compatibility). The housing for the reusable sensor version can be modified to solely accommodate single-use sensors; please contact our Applications Engineering team for the service details.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">What are the recommended sampling and harvesting methods?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">For Mini vessels, the cap on top can be unscrewed for pipetting in a biosafety hood. For the 3L bioreactor, media can be withdrawn from a sample line connected to the vessel via an automated pump or manually. For the 15L and 80L bioreactors, the adjustable height harvest valve can fulfill both functions.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">How can I monitor process parameters during a cell culture run?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">The 3L bioreactor’s software allows for customizable programming of process controls and real-time monitoring of parameters using various sensors. Feedback loops can be used to pause a run based on deviations from a set range. </p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">Can I control and monitor my cell culture process remotely?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">Yes, the software on 3L and larger size bioreactors allows for remote process monitoring, adjustments, and alarm reporting over a secure connection to various devices.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">How is process-related data stored and accessed?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">The software can customize how often and what kind of data will be stored on the bioreactor’s on-board hard drive. Automatic downloading of data can be directed to a local computer with direct LAN connection. All user logins, process changes, and data histories follow 21 CFR Part 11 requirements for cGMP.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">Can the 3L Vertical-Wheel bioreactor fit on top of a standard size lab bench?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">Yes, the 3L bioreactor is designed to be place on a lab bench, rolling cart, or similar platform. Technical specifications such as dimensions and weight are available on the 3L product page.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">How do I know the number and types of Vertical-Wheel bioreactors I will need?</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);transform-style:preserve-3d" class="faq-circle"><img src="https://cdn.prod.website-files.com/62cc627d7fe73059b1484e97/62cc627d7fe730298c484ee4_faq-icon.svg" loading="lazy" alt="faq icon" class="faq-arrow"/></div></div><div style="height:0px" class="faq-content"><p class="faq-answer text-color-blue margin-bottom margin-custom2">Please use the Contact form to describe your process requirements and our Applications Engineering team will be able to assist with your planning.</p></div></div></div><div role="listitem" class="w-dyn-item"><div data-w-id="2e0c66ad-efa6-366a-fe09-74dc162eff99" class="faq-wrapper"><div class="faq-question-bar"><div class="faq-question text-weight-light margin-right margin-xxsmall">I have other questions regarding usage or troubleshooting of the PBS Mini.</div><div style="-webkit-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-moz-transform:translate3d(0, 0, 0) scale3d(1, 1, 1) rotateX(null) rotateY(null) rotateZ(180deg) skew(0, 0);-ms-transform:translate3d(0, 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