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class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by ridwan hussein</h3></div><div class="js-work-strip profile--work_container" data-work-id="124005394"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/124005394/Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons"><img alt="Research paper thumbnail of Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons" class="work-thumbnail" src="https://attachments.academia-assets.com/118311567/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/124005394/Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons">Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A plants tissue lodge bacteria that do not harm plants body but supports its living, these are en...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cce190988947ff8a65a980eab4298c49" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":118311567,"asset_id":124005394,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/118311567/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="124005394"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="124005394"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 124005394; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=124005394]").text(description); $(".js-view-count[data-work-id=124005394]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 124005394; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='124005394']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 124005394, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cce190988947ff8a65a980eab4298c49" } } $('.js-work-strip[data-work-id=124005394]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":124005394,"title":"Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons","translated_title":"","metadata":{"abstract":"A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs."},"translated_abstract":"A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.","internal_url":"https://www.academia.edu/124005394/Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons","translated_internal_url":"","created_at":"2024-09-19T06:56:57.660-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":324796980,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":118311567,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/118311567/thumbnails/1.jpg","file_name":"bio.20221003.12.pdf","download_url":"https://www.academia.edu/attachments/118311567/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Studies_of_Lodge_Bacteria_on_Lablab_purp.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/118311567/bio.20221003.12-libre.pdf?1726756686=\u0026response-content-disposition=attachment%3B+filename%3DStudies_of_Lodge_Bacteria_on_Lablab_purp.pdf\u0026Expires=1733913315\u0026Signature=Th0Xurubz9u3RlPIYAg4savFIxb63YMX1b7~X~cF8yXw8-AuFTnZor94joCiYtfSCWIqOK0v1umYm4NkBJCbRuMznJHaukxrz96rfa0g3GOBGIuWNIxchVPFSZVlbB-sCpunY1nl57jmFFPYvR6xgVjSUsRrKa26mnbkqkQtLZ2QghIttEAprFw7nbqaAvtDIHMmKwp9YXJrJwZ~tGvEVZNNJE3wNLibC3IYBh82-IdAR6WSx4~dA8gNl2I2lE2o~mD8ApCvKGSQ77WpJSQhGmKtrzRLdCE9rP3AnWVfHml4Fb1aCCz1e~LaCwvcQNAqTpf~MRcXfHDOWQTzupSgsQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.","owner":{"id":324796980,"first_name":"ridwan","middle_initials":null,"last_name":"hussein","page_name":"ridwanhussein13","domain_name":"independent","created_at":"2024-09-16T10:23:04.339-07:00","display_name":"ridwan hussein","url":"https://independent.academia.edu/ridwanhussein13"},"attachments":[{"id":118311567,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/118311567/thumbnails/1.jpg","file_name":"bio.20221003.12.pdf","download_url":"https://www.academia.edu/attachments/118311567/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Studies_of_Lodge_Bacteria_on_Lablab_purp.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/118311567/bio.20221003.12-libre.pdf?1726756686=\u0026response-content-disposition=attachment%3B+filename%3DStudies_of_Lodge_Bacteria_on_Lablab_purp.pdf\u0026Expires=1733913315\u0026Signature=Th0Xurubz9u3RlPIYAg4savFIxb63YMX1b7~X~cF8yXw8-AuFTnZor94joCiYtfSCWIqOK0v1umYm4NkBJCbRuMznJHaukxrz96rfa0g3GOBGIuWNIxchVPFSZVlbB-sCpunY1nl57jmFFPYvR6xgVjSUsRrKa26mnbkqkQtLZ2QghIttEAprFw7nbqaAvtDIHMmKwp9YXJrJwZ~tGvEVZNNJE3wNLibC3IYBh82-IdAR6WSx4~dA8gNl2I2lE2o~mD8ApCvKGSQ77WpJSQhGmKtrzRLdCE9rP3AnWVfHml4Fb1aCCz1e~LaCwvcQNAqTpf~MRcXfHDOWQTzupSgsQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="124005301"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/124005301/Studies_on_the_Potential_of_Rhizopus_species_from_raw_food_and_soil_for_Amylase_Enzyme_Production"><img alt="Research paper thumbnail of Studies on the Potential of Rhizopus species from raw food and soil for Amylase Enzyme Production" class="work-thumbnail" src="https://attachments.academia-assets.com/118311483/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/124005301/Studies_on_the_Potential_of_Rhizopus_species_from_raw_food_and_soil_for_Amylase_Enzyme_Production">Studies on the Potential of Rhizopus species from raw food and soil for Amylase Enzyme Production</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Rhizopus species from potato, millet and soil samples were isolated and screened for their abilit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Rhizopus species from potato, millet and soil samples were isolated and screened for their ability to produce enzyme amylase. Potato and millet were dried, grinded and the samples including soil were serially diluted and spread plated (0.1ml) on sterile potato dextrose agar (PDA) plates incubated at 35掳C for 5days. Colonies were repetitively sub-cultured in order for pure cultures. Fungi Rhizopus was macroscopically and microscopically identified based on standard procedures. Rhizopus species were screened using agar plate method at which hydrolysis zones were observed and measured in millimeter (mm) by meter rule. Enzyme was quantified by solid state fermentation (SSF) during which wheat bran (the substrate/medium) and time (96hrs) was used for production. For enzyme extraction, the mixture of fermented medium and tween 80 (0.1%) was shaken by rotary shaker, squeezed by muslin cloth and filtered through filter paper (whatman No. 1). For enzyme activity determination; crude extract of enzyme was mixed with starch, Sodium Chloride(NaCl) and phosphate buffer and the mixture was incubated in a water bath for 30mins at 40掳C, DNSA was added to stop the reaction, heating the mixture again for 5mins after which distilled water was added and the absorbance 540nm was taken using spectrophotometer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e80b8b3bedf1c7f5f3685b44496e77e3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":118311483,"asset_id":124005301,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/118311483/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="124005301"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="124005301"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 124005301; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=124005301]").text(description); $(".js-view-count[data-work-id=124005301]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 124005301; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='124005301']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 124005301, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e80b8b3bedf1c7f5f3685b44496e77e3" } } $('.js-work-strip[data-work-id=124005301]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":124005301,"title":"Studies on the Potential of Rhizopus species from raw food and soil for Amylase Enzyme Production","translated_title":"","metadata":{"abstract":"Rhizopus species from potato, millet and soil samples were isolated and screened for their ability to produce enzyme amylase. Potato and millet were dried, grinded and the samples including soil were serially diluted and spread plated (0.1ml) on sterile potato dextrose agar (PDA) plates incubated at 35掳C for 5days. Colonies were repetitively sub-cultured in order for pure cultures. Fungi Rhizopus was macroscopically and microscopically identified based on standard procedures. Rhizopus species were screened using agar plate method at which hydrolysis zones were observed and measured in millimeter (mm) by meter rule. Enzyme was quantified by solid state fermentation (SSF) during which wheat bran (the substrate/medium) and time (96hrs) was used for production. For enzyme extraction, the mixture of fermented medium and tween 80 (0.1%) was shaken by rotary shaker, squeezed by muslin cloth and filtered through filter paper (whatman No. 1). For enzyme activity determination; crude extract of enzyme was mixed with starch, Sodium Chloride(NaCl) and phosphate buffer and the mixture was incubated in a water bath for 30mins at 40掳C, DNSA was added to stop the reaction, heating the mixture again for 5mins after which distilled water was added and the absorbance 540nm was taken using spectrophotometer."},"translated_abstract":"Rhizopus species from potato, millet and soil samples were isolated and screened for their ability to produce enzyme amylase. Potato and millet were dried, grinded and the samples including soil were serially diluted and spread plated (0.1ml) on sterile potato dextrose agar (PDA) plates incubated at 35掳C for 5days. Colonies were repetitively sub-cultured in order for pure cultures. Fungi Rhizopus was macroscopically and microscopically identified based on standard procedures. Rhizopus species were screened using agar plate method at which hydrolysis zones were observed and measured in millimeter (mm) by meter rule. Enzyme was quantified by solid state fermentation (SSF) during which wheat bran (the substrate/medium) and time (96hrs) was used for production. For enzyme extraction, the mixture of fermented medium and tween 80 (0.1%) was shaken by rotary shaker, squeezed by muslin cloth and filtered through filter paper (whatman No. 1). 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Potato and millet were dried, grinded and the samples including soil were serially diluted and spread plated (0.1ml) on sterile potato dextrose agar (PDA) plates incubated at 35掳C for 5days. Colonies were repetitively sub-cultured in order for pure cultures. Fungi Rhizopus was macroscopically and microscopically identified based on standard procedures. Rhizopus species were screened using agar plate method at which hydrolysis zones were observed and measured in millimeter (mm) by meter rule. Enzyme was quantified by solid state fermentation (SSF) during which wheat bran (the substrate/medium) and time (96hrs) was used for production. For enzyme extraction, the mixture of fermented medium and tween 80 (0.1%) was shaken by rotary shaker, squeezed by muslin cloth and filtered through filter paper (whatman No. 1). For enzyme activity determination; crude extract of enzyme was mixed with starch, Sodium Chloride(NaCl) and phosphate buffer and the mixture was incubated in a water bath for 30mins at 40掳C, DNSA was added to stop the reaction, heating the mixture again for 5mins after which distilled water was added and the absorbance 540nm was taken using spectrophotometer.","owner":{"id":324796980,"first_name":"ridwan","middle_initials":null,"last_name":"hussein","page_name":"ridwanhussein13","domain_name":"independent","created_at":"2024-09-16T10:23:04.339-07:00","display_name":"ridwan hussein","url":"https://independent.academia.edu/ridwanhussein13"},"attachments":[{"id":118311483,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/118311483/thumbnails/1.jpg","file_name":"3_252_Yusuf.pdf","download_url":"https://www.academia.edu/attachments/118311483/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Studies_on_the_Potential_of_Rhizopus_spe.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/118311483/3_252_Yusuf-libre.pdf?1726756700=\u0026response-content-disposition=attachment%3B+filename%3DStudies_on_the_Potential_of_Rhizopus_spe.pdf\u0026Expires=1733913315\u0026Signature=cWgG3iCqwB38mbxH2txSWw2chYp4iJKUBxLrQf8Qh74EcqgdImD3cd927bPxCcT8krSa8VbQj~2C0LV-yzioUx0yK60PeeLvqyolPLF2F0qgH-fDmMFSTXOgKZBbY~Xa~c9VVMSyX2cEKY8Ry7XDQtWU8STM483XTDG~RsfxGUSNEFsKW9F1~JAur9jH0LC~CTP88Opto1CsMMDLPDBBWhDO2i8Dxb7klDab9S2fQyglkaP0PmrJWVh3b7ym4ERFAVw-WLIhMJfy3FV9iJs5QuxYpeiViQGLaRlXETD5NrEVIBmqYj-u4hrwsy6rClVtF3UYUY8eQksb6QGJ9-x1BA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":61742,"name":"Microbial Enzymes","url":"https://www.academia.edu/Documents/in/Microbial_Enzymes"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="20014564" id="papers"><div class="js-work-strip profile--work_container" data-work-id="124005394"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/124005394/Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons"><img alt="Research paper thumbnail of Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons" class="work-thumbnail" src="https://attachments.academia-assets.com/118311567/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/124005394/Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons">Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A plants tissue lodge bacteria that do not harm plants body but supports its living, these are en...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cce190988947ff8a65a980eab4298c49" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":118311567,"asset_id":124005394,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/118311567/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="124005394"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="124005394"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 124005394; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=124005394]").text(description); $(".js-view-count[data-work-id=124005394]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 124005394; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='124005394']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 124005394, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "cce190988947ff8a65a980eab4298c49" } } $('.js-work-strip[data-work-id=124005394]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":124005394,"title":"Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons","translated_title":"","metadata":{"abstract":"A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs."},"translated_abstract":"A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.","internal_url":"https://www.academia.edu/124005394/Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons","translated_internal_url":"","created_at":"2024-09-19T06:56:57.660-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":324796980,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":118311567,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/118311567/thumbnails/1.jpg","file_name":"bio.20221003.12.pdf","download_url":"https://www.academia.edu/attachments/118311567/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Studies_of_Lodge_Bacteria_on_Lablab_purp.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/118311567/bio.20221003.12-libre.pdf?1726756686=\u0026response-content-disposition=attachment%3B+filename%3DStudies_of_Lodge_Bacteria_on_Lablab_purp.pdf\u0026Expires=1733913315\u0026Signature=Th0Xurubz9u3RlPIYAg4savFIxb63YMX1b7~X~cF8yXw8-AuFTnZor94joCiYtfSCWIqOK0v1umYm4NkBJCbRuMznJHaukxrz96rfa0g3GOBGIuWNIxchVPFSZVlbB-sCpunY1nl57jmFFPYvR6xgVjSUsRrKa26mnbkqkQtLZ2QghIttEAprFw7nbqaAvtDIHMmKwp9YXJrJwZ~tGvEVZNNJE3wNLibC3IYBh82-IdAR6WSx4~dA8gNl2I2lE2o~mD8ApCvKGSQ77WpJSQhGmKtrzRLdCE9rP3AnWVfHml4Fb1aCCz1e~LaCwvcQNAqTpf~MRcXfHDOWQTzupSgsQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Studies_of_Lodge_Bacteria_on_Lablab_purpureau_and_Pennisetum_hybridum_for_Potential_Degradation_of_Polycyclic_Aromatic_Hydrocarbons","translated_slug":"","page_count":5,"language":"en","content_type":"Work","summary":"A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0脳10 4 cfu/g and Pennisetum hybridum (KgG) 3.2脳10 5 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36卤1掳C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.","owner":{"id":324796980,"first_name":"ridwan","middle_initials":null,"last_name":"hussein","page_name":"ridwanhussein13","domain_name":"independent","created_at":"2024-09-16T10:23:04.339-07:00","display_name":"ridwan hussein","url":"https://independent.academia.edu/ridwanhussein13"},"attachments":[{"id":118311567,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/118311567/thumbnails/1.jpg","file_name":"bio.20221003.12.pdf","download_url":"https://www.academia.edu/attachments/118311567/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Studies_of_Lodge_Bacteria_on_Lablab_purp.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/118311567/bio.20221003.12-libre.pdf?1726756686=\u0026response-content-disposition=attachment%3B+filename%3DStudies_of_Lodge_Bacteria_on_Lablab_purp.pdf\u0026Expires=1733913315\u0026Signature=Th0Xurubz9u3RlPIYAg4savFIxb63YMX1b7~X~cF8yXw8-AuFTnZor94joCiYtfSCWIqOK0v1umYm4NkBJCbRuMznJHaukxrz96rfa0g3GOBGIuWNIxchVPFSZVlbB-sCpunY1nl57jmFFPYvR6xgVjSUsRrKa26mnbkqkQtLZ2QghIttEAprFw7nbqaAvtDIHMmKwp9YXJrJwZ~tGvEVZNNJE3wNLibC3IYBh82-IdAR6WSx4~dA8gNl2I2lE2o~mD8ApCvKGSQ77WpJSQhGmKtrzRLdCE9rP3AnWVfHml4Fb1aCCz1e~LaCwvcQNAqTpf~MRcXfHDOWQTzupSgsQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="124005301"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/124005301/Studies_on_the_Potential_of_Rhizopus_species_from_raw_food_and_soil_for_Amylase_Enzyme_Production"><img alt="Research paper thumbnail of Studies on the Potential of Rhizopus species from raw food and soil for Amylase Enzyme Production" class="work-thumbnail" src="https://attachments.academia-assets.com/118311483/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/124005301/Studies_on_the_Potential_of_Rhizopus_species_from_raw_food_and_soil_for_Amylase_Enzyme_Production">Studies on the Potential of Rhizopus species from raw food and soil for Amylase Enzyme Production</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Rhizopus species from potato, millet and soil samples were isolated and screened for their abilit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Rhizopus species from potato, millet and soil samples were isolated and screened for their ability to produce enzyme amylase. Potato and millet were dried, grinded and the samples including soil were serially diluted and spread plated (0.1ml) on sterile potato dextrose agar (PDA) plates incubated at 35掳C for 5days. Colonies were repetitively sub-cultured in order for pure cultures. Fungi Rhizopus was macroscopically and microscopically identified based on standard procedures. Rhizopus species were screened using agar plate method at which hydrolysis zones were observed and measured in millimeter (mm) by meter rule. Enzyme was quantified by solid state fermentation (SSF) during which wheat bran (the substrate/medium) and time (96hrs) was used for production. For enzyme extraction, the mixture of fermented medium and tween 80 (0.1%) was shaken by rotary shaker, squeezed by muslin cloth and filtered through filter paper (whatman No. 1). For enzyme activity determination; crude extract of enzyme was mixed with starch, Sodium Chloride(NaCl) and phosphate buffer and the mixture was incubated in a water bath for 30mins at 40掳C, DNSA was added to stop the reaction, heating the mixture again for 5mins after which distilled water was added and the absorbance 540nm was taken using spectrophotometer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e80b8b3bedf1c7f5f3685b44496e77e3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":118311483,"asset_id":124005301,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/118311483/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="124005301"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="124005301"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 124005301; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=124005301]").text(description); $(".js-view-count[data-work-id=124005301]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 124005301; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='124005301']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 124005301, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e80b8b3bedf1c7f5f3685b44496e77e3" } } $('.js-work-strip[data-work-id=124005301]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":124005301,"title":"Studies on the Potential of Rhizopus species from raw food and soil for Amylase Enzyme Production","translated_title":"","metadata":{"abstract":"Rhizopus species from potato, millet and soil samples were isolated and screened for their ability to produce enzyme amylase. Potato and millet were dried, grinded and the samples including soil were serially diluted and spread plated (0.1ml) on sterile potato dextrose agar (PDA) plates incubated at 35掳C for 5days. Colonies were repetitively sub-cultured in order for pure cultures. Fungi Rhizopus was macroscopically and microscopically identified based on standard procedures. Rhizopus species were screened using agar plate method at which hydrolysis zones were observed and measured in millimeter (mm) by meter rule. Enzyme was quantified by solid state fermentation (SSF) during which wheat bran (the substrate/medium) and time (96hrs) was used for production. For enzyme extraction, the mixture of fermented medium and tween 80 (0.1%) was shaken by rotary shaker, squeezed by muslin cloth and filtered through filter paper (whatman No. 1). For enzyme activity determination; crude extract of enzyme was mixed with starch, Sodium Chloride(NaCl) and phosphate buffer and the mixture was incubated in a water bath for 30mins at 40掳C, DNSA was added to stop the reaction, heating the mixture again for 5mins after which distilled water was added and the absorbance 540nm was taken using spectrophotometer."},"translated_abstract":"Rhizopus species from potato, millet and soil samples were isolated and screened for their ability to produce enzyme amylase. Potato and millet were dried, grinded and the samples including soil were serially diluted and spread plated (0.1ml) on sterile potato dextrose agar (PDA) plates incubated at 35掳C for 5days. Colonies were repetitively sub-cultured in order for pure cultures. Fungi Rhizopus was macroscopically and microscopically identified based on standard procedures. Rhizopus species were screened using agar plate method at which hydrolysis zones were observed and measured in millimeter (mm) by meter rule. Enzyme was quantified by solid state fermentation (SSF) during which wheat bran (the substrate/medium) and time (96hrs) was used for production. For enzyme extraction, the mixture of fermented medium and tween 80 (0.1%) was shaken by rotary shaker, squeezed by muslin cloth and filtered through filter paper (whatman No. 1). For enzyme activity determination; crude extract of enzyme was mixed with starch, Sodium Chloride(NaCl) and phosphate buffer and the mixture was incubated in a water bath for 30mins at 40掳C, DNSA was added to stop the reaction, heating the mixture again for 5mins after which distilled water was added and the absorbance 540nm was taken using spectrophotometer.","internal_url":"https://www.academia.edu/124005301/Studies_on_the_Potential_of_Rhizopus_species_from_raw_food_and_soil_for_Amylase_Enzyme_Production","translated_internal_url":"","created_at":"2024-09-19T06:52:15.792-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":324796980,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":118311483,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/118311483/thumbnails/1.jpg","file_name":"3_252_Yusuf.pdf","download_url":"https://www.academia.edu/attachments/118311483/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Studies_on_the_Potential_of_Rhizopus_spe.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/118311483/3_252_Yusuf-libre.pdf?1726756700=\u0026response-content-disposition=attachment%3B+filename%3DStudies_on_the_Potential_of_Rhizopus_spe.pdf\u0026Expires=1733913315\u0026Signature=cWgG3iCqwB38mbxH2txSWw2chYp4iJKUBxLrQf8Qh74EcqgdImD3cd927bPxCcT8krSa8VbQj~2C0LV-yzioUx0yK60PeeLvqyolPLF2F0qgH-fDmMFSTXOgKZBbY~Xa~c9VVMSyX2cEKY8Ry7XDQtWU8STM483XTDG~RsfxGUSNEFsKW9F1~JAur9jH0LC~CTP88Opto1CsMMDLPDBBWhDO2i8Dxb7klDab9S2fQyglkaP0PmrJWVh3b7ym4ERFAVw-WLIhMJfy3FV9iJs5QuxYpeiViQGLaRlXETD5NrEVIBmqYj-u4hrwsy6rClVtF3UYUY8eQksb6QGJ9-x1BA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Studies_on_the_Potential_of_Rhizopus_species_from_raw_food_and_soil_for_Amylase_Enzyme_Production","translated_slug":"","page_count":6,"language":"en","content_type":"Work","summary":"Rhizopus species from potato, millet and soil samples were isolated and screened for their ability to produce enzyme amylase. 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For enzyme activity determination; crude extract of enzyme was mixed with starch, Sodium Chloride(NaCl) and phosphate buffer and the mixture was incubated in a water bath for 30mins at 40掳C, DNSA was added to stop the reaction, heating the mixture again for 5mins after which distilled water was added and the absorbance 540nm was taken using spectrophotometer.","owner":{"id":324796980,"first_name":"ridwan","middle_initials":null,"last_name":"hussein","page_name":"ridwanhussein13","domain_name":"independent","created_at":"2024-09-16T10:23:04.339-07:00","display_name":"ridwan hussein","url":"https://independent.academia.edu/ridwanhussein13"},"attachments":[{"id":118311483,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/118311483/thumbnails/1.jpg","file_name":"3_252_Yusuf.pdf","download_url":"https://www.academia.edu/attachments/118311483/download_file?st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&st=MTczMzkwOTcxNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Studies_on_the_Potential_of_Rhizopus_spe.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/118311483/3_252_Yusuf-libre.pdf?1726756700=\u0026response-content-disposition=attachment%3B+filename%3DStudies_on_the_Potential_of_Rhizopus_spe.pdf\u0026Expires=1733913315\u0026Signature=cWgG3iCqwB38mbxH2txSWw2chYp4iJKUBxLrQf8Qh74EcqgdImD3cd927bPxCcT8krSa8VbQj~2C0LV-yzioUx0yK60PeeLvqyolPLF2F0qgH-fDmMFSTXOgKZBbY~Xa~c9VVMSyX2cEKY8Ry7XDQtWU8STM483XTDG~RsfxGUSNEFsKW9F1~JAur9jH0LC~CTP88Opto1CsMMDLPDBBWhDO2i8Dxb7klDab9S2fQyglkaP0PmrJWVh3b7ym4ERFAVw-WLIhMJfy3FV9iJs5QuxYpeiViQGLaRlXETD5NrEVIBmqYj-u4hrwsy6rClVtF3UYUY8eQksb6QGJ9-x1BA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":61742,"name":"Microbial Enzymes","url":"https://www.academia.edu/Documents/in/Microbial_Enzymes"}],"urls":[]}, dispatcherData: dispatcherData }); 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