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Ghavamzadeh</title> <meta name="description" content="Search results for: Ardeshir Ghavamzadeh"> <meta name="keywords" content="Ardeshir Ghavamzadeh"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Ardeshir Ghavamzadeh"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 7</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Ardeshir Ghavamzadeh</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Potential Drug-Drug Interactions at a Referral Hematology-Oncology Ward in Iran: A Cross-Sectional Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sara%20Ataei">Sara Ataei</a>, <a href="https://publications.waset.org/abstracts/search?q=Molouk%20Hadjibabaie"> Molouk Hadjibabaie</a>, <a href="https://publications.waset.org/abstracts/search?q=Shirinsadat%20Badri"> Shirinsadat Badri</a>, <a href="https://publications.waset.org/abstracts/search?q=Amirhossein%20Moslehi"> Amirhossein Moslehi</a>, <a href="https://publications.waset.org/abstracts/search?q=Iman%20Karimzadeh"> Iman Karimzadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Ardeshir%20Ghavamzadeh"> Ardeshir Ghavamzadeh </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: To assess the pattern and probable risk factors for moderate and major drug–drug interactions in a referral hematology-oncology ward in Iran. Methods: All patients admitted to hematology–oncology ward of Dr. Shariati Hospital during a 6-month period and received at least two anti-cancer or non-anti-cancer medications simultaneously were included. All being scheduled anti-cancer and non-anti-cancer medications both prescribed and administered during ward stay were considered for drug–drug interaction screening by Lexi-Interact On- Desktop software. Results: One hundred and eighty-five drug–drug interactions with moderate or major severity were detected from 83 patients. Most of drug–drug interactions (69.73 %) were classified as pharmacokinetics. Fluconazole (25.95 %) was the most commonly offending medication in drug–drug interactions. Interaction of sulfamethoxazole-trimethoprim with fluconazole was the most common drug–drug interaction (27.27 %). Vincristine with imatinib was the only identified interaction between two anti-cancer agents. The number of administered medications during ward stay was considered as an independent risk factor for developing a drug–drug interaction. Conclusions: Potential moderate or major drug–drug interactions occur frequently in patients with hematological malignancies or related diseases. Performing larger standard studies are required to assess the real clinical and economical effects of drug–drug interactions on patients with hematological and non-hematological malignancies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%E2%80%93drug%20interactions" title="drug–drug interactions">drug–drug interactions</a>, <a href="https://publications.waset.org/abstracts/search?q=hematology%E2%80%93oncology%20ward" title=" hematology–oncology ward"> hematology–oncology ward</a>, <a href="https://publications.waset.org/abstracts/search?q=hematological%20malignancies" title=" hematological malignancies "> hematological malignancies </a> </p> <a href="https://publications.waset.org/abstracts/17983/potential-drug-drug-interactions-at-a-referral-hematology-oncology-ward-in-iran-a-cross-sectional-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17983.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">454</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> A Double-Blind, Randomized, Controlled Trial on N-Acetylcysteine for the Prevention of Acute Kidney Injury in Patients Undergoing Allogeneic Hematopoietic Stem Cell Transplantation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sara%20Ataei">Sara Ataei</a>, <a href="https://publications.waset.org/abstracts/search?q=Molouk%20Hadjibabaie"> Molouk Hadjibabaie</a>, <a href="https://publications.waset.org/abstracts/search?q=Amirhossein%20Moslehi"> Amirhossein Moslehi</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Taghizadeh-Ghehi"> Maryam Taghizadeh-Ghehi</a>, <a href="https://publications.waset.org/abstracts/search?q=Asieh%20Ashouri"> Asieh Ashouri</a>, <a href="https://publications.waset.org/abstracts/search?q=Elham%20Amini"> Elham Amini</a>, <a href="https://publications.waset.org/abstracts/search?q=Kheirollah%20Gholami"> Kheirollah Gholami</a>, <a href="https://publications.waset.org/abstracts/search?q=Alireza%20Hayatshahi"> Alireza Hayatshahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Vaezi"> Mohammad Vaezi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ardeshir%20Ghavamzadeh">Ardeshir Ghavamzadeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acute kidney injury (AKI) is one of the complications of hematopoietic stem cell transplantation and is associated with increased mortality. N-acetylcysteine (NAC) is a thiol compound with antioxidant and vasodilatory properties that has been investigated for the prevention of AKI in several clinical settings. In the present study, we evaluated the effects of intravenous NAC on the prevention of AKI in allogeneic hematopoietic stem cell transplantation patients. A double-blind randomized placebo-controlled trial was conducted, and 80 patients were recruited to receive 100 mg/kg/day NAC or placebo as intermittent intravenous infusion from day -6 to day +15. AKI was determined on the basis of the Risk-Injury-Failure-Loss-Endstage renal disease and AKI Network criteria as the primary outcome. We assessed urine neutrophil gelatinase-associated lipocalin (uNGAL) on days -6, -3, +3, +9, and +15 as the secondary outcome. Moreover, transplant-related outcomes and NAC adverse reactions were evaluated during the study period. Statistical analysis was performed using appropriate parametric and non-parametric methods including Kaplan–Meier for AKI and generalized estimating equation for uNGAL. At the end of the trial, data from 72 patients were analyzed (NAC: 33 patients and placebo: 39 patients). Participants of each group were not different considering baseline characteristics. AKI was observed in 18% of NAC recipients and 15% of placebo group patients, and the occurrence pattern was not significantly different (p = 0.73). Moreover, no significant difference was observed between groups for uNGAL measures (p = 0.10). Transplant-related outcomes were similar for both groups, and all patients had successful engraftment. Three patients did not tolerate NAC because of abdominal pain, shortness of breath and rash with pruritus and were dropped from the intervention group before transplantation. However, the frequency of adverse reactions was not significantly different between groups. In conclusion, our findings could not show any clinical benefits from high-dose NAC particularly for AKI prevention in allogeneic hematopoietic stem cell transplantation patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acute%20kidney%20injury" title="acute kidney injury">acute kidney injury</a>, <a href="https://publications.waset.org/abstracts/search?q=N-acetylcysteine" title=" N-acetylcysteine"> N-acetylcysteine</a>, <a href="https://publications.waset.org/abstracts/search?q=hematopoietic%20stem%20cell%20transplantation" title=" hematopoietic stem cell transplantation"> hematopoietic stem cell transplantation</a>, <a href="https://publications.waset.org/abstracts/search?q=urine%20neutrophil%20gelatinase-associated%20lipocalin" title=" urine neutrophil gelatinase-associated lipocalin"> urine neutrophil gelatinase-associated lipocalin</a>, <a href="https://publications.waset.org/abstracts/search?q=randomized%20controlled%20trial" title=" randomized controlled trial"> randomized controlled trial</a> </p> <a href="https://publications.waset.org/abstracts/17971/a-double-blind-randomized-controlled-trial-on-n-acetylcysteine-for-the-prevention-of-acute-kidney-injury-in-patients-undergoing-allogeneic-hematopoietic-stem-cell-transplantation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17971.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">433</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> A Low Insertion Loss Variation 10-35 GHz Phase Shifter</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Soroush%20Rasti%20Boroujeni">Soroush Rasti Boroujeni</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Hassan%20Mousavi"> S. Hassan Mousavi</a>, <a href="https://publications.waset.org/abstracts/search?q=Javad%20Ebrahimizadeh"> Javad Ebrahimizadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Ardeshir%20Palizban"> Ardeshir Palizban</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad-Reza%20Nezhad-Ahmadi"> Mohammad-Reza Nezhad-Ahmadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Safieddin%20Safavi-Naeini"> Safieddin Safavi-Naeini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper presents a wideband True Time Delay (TTD) phase shifter with low insertion loss variation. The circuit benefits from a controllable resistive load shunt with transmission line segments to optimize return loss variations, addressing the unbalanced capacitive nature of the varactor. The phase shifter reduces the complexity of the calibration process because the dependency of insertion loss on voltage controls is improved up to 3 dB. The TTD phase shifter provides a continuous changing delay time of 6.4 ps with low insertion loss (IL) in the 10-35 GHz frequency range. The proposed circuit benefits from lowloss phase shifters with a small footprint. Fabricated using a 65 nm CMOC process, the TTD phase shifter occupies only 388 × 615 μm² of chip area, achieving a 20% improvements compared to conventional TTD phase shifters. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=millimeter-wave%20phased-array" title="millimeter-wave phased-array">millimeter-wave phased-array</a>, <a href="https://publications.waset.org/abstracts/search?q=true%20time%20delay%20phase%20shifter" title=" true time delay phase shifter"> true time delay phase shifter</a>, <a href="https://publications.waset.org/abstracts/search?q=insertion%20loss%20variation" title=" insertion loss variation"> insertion loss variation</a>, <a href="https://publications.waset.org/abstracts/search?q=compact%20size" title=" compact size"> compact size</a> </p> <a href="https://publications.waset.org/abstracts/193319/a-low-insertion-loss-variation-10-35-ghz-phase-shifter" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193319.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">7</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Sensitivity Analysis of Oil Spills Modeling with ADIOS II for Iranian Fields in Persian Gulf</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Farzingohar%20Mehrnaz">Farzingohar Mehrnaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Yasemi%20Mehran"> Yasemi Mehran</a>, <a href="https://publications.waset.org/abstracts/search?q=Esmaili%20Zinat"> Esmaili Zinat</a>, <a href="https://publications.waset.org/abstracts/search?q=Baharlouian%20Maedeh"> Baharlouian Maedeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aboozar (Ardeshir) and Bahregansar are the two important Iranian oilfields in Persian Gulf waters. The operation activities cause to create spills which impacted on the marine environment. Assumed spills are molded by ADIOS II (Automated Data Inquiry for Oil Spills) which is NOAA’s weathering oil software. Various atmospheric and marine data with different oil types are used for the modeling. Numerous scenarios for 100 bbls with mean daily air temperature and wind speed are input for 5 days. To find the model sensitivity in each setting, one parameter is changed, but the others stayed constant. In both fields, the evaporated and dispersed output values increased hence the remaining rate is reduced. The results clarified that wind speed first, second air temperature and finally oil type respectively were the most effective factors on the oil weathering process. The obtained results can help the emergency systems to predict the floating (dispersed and remained) volume spill in order to find the suitable cleanup tools and methods. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ADIOS" title="ADIOS">ADIOS</a>, <a href="https://publications.waset.org/abstracts/search?q=modeling" title=" modeling"> modeling</a>, <a href="https://publications.waset.org/abstracts/search?q=oil%20spill" title=" oil spill"> oil spill</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity%20analysis" title=" sensitivity analysis"> sensitivity analysis</a> </p> <a href="https://publications.waset.org/abstracts/46836/sensitivity-analysis-of-oil-spills-modeling-with-adios-ii-for-iranian-fields-in-persian-gulf" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46836.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">299</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Major Dietary Patterns in Relationship with Anthropometric Indices in North West of Iran</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arezou%20Rezazadeh">Arezou Rezazadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Nasrin%20Omidvar"> Nasrin Omidvar</a>, <a href="https://publications.waset.org/abstracts/search?q=Hassan%20Eini-Zinab"> Hassan Eini-Zinab</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmoud%20Ghazi-Tabatabaie"> Mahmoud Ghazi-Tabatabaie</a>, <a href="https://publications.waset.org/abstracts/search?q=Reza%20Majdzadeh"> Reza Majdzadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Saeid%20Ghavamzadeh"> Saeid Ghavamzadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Sakineh%20Nouri-Saeidlou"> Sakineh Nouri-Saeidlou</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dietary pattern analysis method can reflect more information about the nutritional etiology of chronic diseases such as obesity. The aim of this study was to determine the relationship between major dietary patterns and anthropometric measures in men and women living in the city of Urmia. In this cross-sectional study, 723 participants (427 women and 296 men), aged 20–64 in Urmia city were selected from all four zones of Urmia city, in the north-west of Iran. Anthropometrics (weight, height, waist and hip circumference) were measured with standard methods. Body Mass Index (BMI) was calculated by dividing weight (in kilograms) by the square of height (in meter). Dietary intake information was collected by a semi-quantitative food frequency questionnaire in the last year. Dietary patterns were determined using principal component analysis. The relationship between dietary patterns and obesity was analyzed by logistic regression. Three major dietary patterns (DPs) were identified that were named ‘Traditional Higher SES (THS)’, ‘Traditional Low SES (TLS)’ and ‘Transitional’. THS DP was positively and Transitional DP was negatively associated with BMI and waist circumference (W.C), however, after adjusting for confounding variables (age, gender, ethnicity, energy intake, physical activity and SES), the associations were not significant. The TLS was not significantly associated with BMI, but after adjusting for confounders, a significant positive association was detected with W.C and Waist to hip ratio (WHR). Findings showed that both traditional patterns were positively and the western type transitional pattern was reversely associated with anthropometric indices. But this relationship was highly affected by demographic, socioeconomic and energy input and output determinants. The results indicate the inevitable effect of environmental factors on the relationship between dietary patterns and anthropometric indices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anthropometric%20indices" title="anthropometric indices">anthropometric indices</a>, <a href="https://publications.waset.org/abstracts/search?q=dietary%20pattern" title=" dietary pattern"> dietary pattern</a>, <a href="https://publications.waset.org/abstracts/search?q=Iran" title=" Iran"> Iran</a>, <a href="https://publications.waset.org/abstracts/search?q=North-west" title=" North-west"> North-west</a> </p> <a href="https://publications.waset.org/abstracts/96680/major-dietary-patterns-in-relationship-with-anthropometric-indices-in-north-west-of-iran" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96680.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">165</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Analysis of the Current and Ideal Situation of Iran’s Football Talent Management Process from the Perspective of the Elites</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mehran%20Nasiri">Mehran Nasiri</a>, <a href="https://publications.waset.org/abstracts/search?q=Ardeshir%20Poornemat"> Ardeshir Poornemat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study was to investigate the current and ideal situations of the process of talent identification in Iranian football from the point of view of Iranian instructors of the Asian Football Confederation (AFC). This research was a descriptive-analytical study; in data collection phase a questionnaire was used, whose face validity was confirmed by experts of Physical Education and Sports Science. The reliability of questionnaire was estimated through the use of Cronbach's alpha method (0.91). This study involved 122 participants of Iranian instructors of the AFC who were selected based on stratified random sampling method. Descriptive statistics were used to describe the variables and inferential statistics (Chi-square) were used to test the hypotheses of the study at significant level (p ≤ 0.05). The results of Chi-square test related to the point of view of Iranian instructors of the AFC showed that the grass-roots scientific method was the best way to identify football players (0.001), less than 10 years old were the best ages for talent identification (0.001), the Football Federation was revealed to be the most important organization in talent identification (0.002), clubs were shown to be the most important institution in developing talents (0.001), trained scouts of Football Federation were demonstrated to be the best and most appropriate group for talent identification (0.001), and being referred by the football academy coaches was shown to be the best way to attract talented football players in Iran (0.001). It was also found that there was a huge difference between the current and ideal situation of the process of talent identification in Iranian football from the point of view of Iranian instructors of the AFC. Hence, it is recommended that the policy makers of talent identification for Iranian football provide a comprehensive, clear and systematic model of talent identification and development processes for the clubs and football teams, so that the talent identification process helps to nurture football talents more efficiently. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=current%20situation" title="current situation">current situation</a>, <a href="https://publications.waset.org/abstracts/search?q=talent%20finding" title=" talent finding"> talent finding</a>, <a href="https://publications.waset.org/abstracts/search?q=ideal%20situation" title=" ideal situation"> ideal situation</a>, <a href="https://publications.waset.org/abstracts/search?q=instructors%20%28AFC%29" title=" instructors (AFC)"> instructors (AFC)</a> </p> <a href="https://publications.waset.org/abstracts/69682/analysis-of-the-current-and-ideal-situation-of-irans-football-talent-management-process-from-the-perspective-of-the-elites" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/69682.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">213</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Comparative Proteomic Profiling of Planktonic and Biofilms from Staphylococcus aureus Using Tandem Mass Tag-Based Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arifur%20Rahman">Arifur Rahman</a>, <a href="https://publications.waset.org/abstracts/search?q=Ardeshir%20Amirkhani"> Ardeshir Amirkhani</a>, <a href="https://publications.waset.org/abstracts/search?q=Honghua%20Hu"> Honghua Hu</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20Molloy"> Mark Molloy</a>, <a href="https://publications.waset.org/abstracts/search?q=Karen%20Vickery"> Karen Vickery</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction and Objectives: Staphylococcus aureus and coagulase-negative staphylococci comprises approximately 65% of infections associated with medical devices and are well known for their biofilm formatting ability. Biofilm-related infections are extremely difficult to eradicate owing to their high tolerance to antibiotics and host immune defences. Currently, there is no efficient method for early biofilm detection. A better understanding to enable detection of biofilm specific proteins in vitro and in vivo can be achieved by studying planktonic and different growth phases of biofilms using a proteome analysis approach. Our goal was to construct a reference map of planktonic and biofilm associated proteins of S. aureus. Methods: S. aureus reference strain (ATCC 25923) was used to grow 24 hours planktonic, 3-day wet biofilm (3DWB), and 12-day wet biofilm (12DWB). Bacteria were grown in tryptic soy broth (TSB) liquid medium. Planktonic growth was used late logarithmic bacteria, and the Centres for Disease Control (CDC) biofilm reactor was used to grow 3 days, and 12-day hydrated biofilms, respectively. Samples were subjected to reduction, alkylation and digestion steps prior to Multiplex labelling using Tandem Mass Tag (TMT) 10-plex reagent (Thermo Fisher Scientific). The labelled samples were pooled and fractionated by high pH RP-HPLC which followed by loading of the fractions on a nanoflow UPLC system (Eksigent UPLC system, AB SCIEX). Mass spectrometry (MS) data were collected on an Orbitrap Elite (Thermo Fisher Scientific) Mass Spectrometer. Protein identification and relative quantitation of protein levels were performed using Proteome Discoverer (version 1.3, Thermo Fisher Scientific). After the extraction of protein ratios with Proteome Discoverer, additional processing, and statistical analysis was done using the TMTPrePro R package. Results and Discussion: The present study showed that a considerable proteomic difference exists among planktonic and biofilms from S. aureus. We identified 1636 total extracellular secreted proteins, of which 350 and 137 proteins of 3DWB and 12DWB showed significant abundance variation from planktonic preparation, respectively. Of these, simultaneous up-regulation in between 3DWB and 12DWB proteins such as extracellular matrix-binding protein ebh, enolase, transketolase, triosephosphate isomerase, chaperonin, peptidase, pyruvate kinase, hydrolase, aminotransferase, ribosomal protein, acetyl-CoA acetyltransferase, DNA gyrase subunit A, glycine glycyltransferase and others we found in this biofilm producer. On the contrary, simultaneous down-regulation in between 3DWB and 12DWB proteins such as alpha and delta-hemolysin, lipoteichoic acid synthase, enterotoxin I, serine protease, lipase, clumping factor B, regulatory protein Spx, phosphoglucomutase, and others also we found in this biofilm producer. In addition, we also identified a big percentage of hypothetical proteins including unique proteins. Therefore, a comprehensive knowledge of planktonic and biofilm associated proteins identified by S. aureus will provide a basis for future studies on the development of vaccines and diagnostic biomarkers. Conclusions: In this study, we constructed an initial reference map of planktonic and various growth phase of biofilm associated proteins which might be helpful to diagnose biofilm associated infections. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacterial%20biofilms" title="bacterial biofilms">bacterial biofilms</a>, <a href="https://publications.waset.org/abstracts/search?q=CDC%20bioreactor" title=" CDC bioreactor"> CDC bioreactor</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20aureus" title=" S. aureus"> S. aureus</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=TMT" title=" TMT"> TMT</a> </p> <a href="https://publications.waset.org/abstracts/77519/comparative-proteomic-profiling-of-planktonic-and-biofilms-from-staphylococcus-aureus-using-tandem-mass-tag-based-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77519.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">171</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th 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