Immune Cell Therapy GMP Reagents, Assays, and Instruments

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Areas</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-therapeutic-manufacturing" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Therapeutic Manufacturing <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-therapeutic-manufacturing" aria-labelledby="navbarDropdown-therapeutic-manufacturing"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Reagents & Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/reagents/cell-culture-reagents/serum-free-animal-free" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3576">Animal-Free Cell Culture</a> <a href="/reagents/elisa-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/176">ELISA Kits</a> <a 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href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a 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aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom 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Bio-Techne" data-drupal-link-system-path="node/1136">Custom Gene Engineering Services</a> <a href="/services/custom-luminex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/271">Custom Luminex Services</a> <a href="/services/custom-protein-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1441">Custom Protein Services</a> <a href="/services/custom-simple-plex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span 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</div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" 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class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_h1"> <div><h1>Immune Cell Therapy</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Mitigate risk and embed flexibility into your immune cell therapy workflow.</p></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_call_to_action_global"> <a href="#workflow" class="btn btn-primary" type="link"> Explore the Workflow </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/tcell-cars-foreground-525x525.png" alt="T Cells with Cars" 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and clinical development programs. The key factors for bringing therapeutics to successful commercialization are:</p> <ul> <li>Lot-to-lot consistency, reproducibility, and scalability</li> <li>Understanding your therapy’s mechanism of action and its interactions in the tumor microenvironment</li> <li>Product characterization, defining the right critical quality attributes and the ability to measure them</li> <li>Accurate efficacy and toxicity testing for the validation of preclinical models </li> </ul> <p>We understand the challenges you face in immune cell therapy development because we’ve been closely collaborating in the field for over 45 years. We commit to working in partnership with you, providing innovative solutions that support you throughout the immune cell therapy workflow. </p> <p>Our pioneering technologies are designed to mitigate risks, boost reproducibility, and enable you to draw the meaningful conclusions you need to drive clinical advances.</p> <p> </p> <p><a name="workflow"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Immune Cell Therapy Workflow</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/ict-workflow-graphic-v2.png" alt="Immune Cell Therapy Workflow" loading="lazy" > </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-immune-cell-culture-and-activation4" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Immune Cell Culture and Activation</h5> </a> </div> <div class="collapse" id="expPara-immune-cell-culture-and-activation4" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><h2>Immune Cell Culture and Activation</h2><p>When looking to expand isolated leukocyte populations to scale, how can you ensure optimal growth conditions for consistent, reproducible lots? The best place to start is by using a reliable supplier that can provide a consistent supply of high-quality cell culture media, additives, and activators. Unlike many others, we manufacture 90% of our products in-house, letting us tightly control the supply chain to help you achieve lot consistency at scale.</p><h3>Featured Solutions</h3><h4>Optimize Your Immune Cell Culture</h4><p>Boost reproducibility in your cell culture with ExCellerate™ media. This highly-defined cell culture medium is optimized for immune cell culture, and is serum- and animal component-free for maximum lot consistency.</p><p><a href="/reagents/cell-culture-reagents/excellerate">View Immune Cell Culture Media</a></p><h4>Animal-Free RUO and GMP Cytokines</h4><p>Accelerate your time to market by improving your culture expansion consistency. We manufacture our animal-free RUO cytokines and growth factors using the same process as our GMP cytokines to ensure they exhibit identical bioactivity to minimize process disruption during your transition to GMP.</p><p><a href="/reagents/proteins/animal-free-proteins">Browse Animal-Free RUO and GMP Cytokines</a></p><h4>GMP Antibodies</h4><p>Unconjugated and unbound anti-CD3 and anti-CD28 offer the necessary flexibility to adapt to various cell therapy systems and ensure optimal phenotype.</p><p><a href="/gmp-products/gmp-antibodies">Learn More About GMP Antibodies</a></p><h4>Verify with Immunoassays</h4><p>Deliver accurate, high-quality data consistently with our highly sensitive and specific immunoassay workflow solutions. Whether you’re quantifying cytokine activation with Ella™ or detecting the activation of intracellular signaling with our Simple Western™ systems, you can count on our systems to ensure the most accurate and reliable results.</p><p><a href="/applications/immunoassays">Discover Immunoassay Solutions</a></p><h4>QC Testing of GMP Proteins</h4><p>Characterization of the quality of raw materials and reagents used during manufacturing is key to ensuring the safety and efficacy of your cell therapy products. Maurice™, an automated capillary electrophoresis instrument, streamlines the characterization of GMP proteins, saving you time, sample, and labor.</p><p><a href="/instruments/ice">Meet Maurice</a></p><h3>Resources</h3><ul><li><a href="/resources/literature/analyze-gmp-proteins-for-cell-therapy-with-maurice">Application Note: Simplify the Qualification of Your GMP Proteins with Maurice</a></li><li><a href="/gmp-products/gmp-facility-tour-request">Video: GMP Manufacturing Facility Virtual Tour</a></li><li><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/fl-key-considerations-for-cytokine-supplier-selection.pdf">Article: Key Considerations for Cytokine Supplier Selection for Cell Therapies</a></li></ul></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-immune-cell-engineering1" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Immune Cell Engineering</h5> </a> </div> <div class="collapse" id="expPara-immune-cell-engineering1" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><h2>Immune Cell Engineering</h2> <p>Cell engineering is one of the most demanding and time-consuming steps of immune cell therapy development. From payload delivery to generating stable protein expression, the cell engineering stage can be a bottleneck. Save time, embed scalability into your process, and move forward with confidence with our cell engineering solutions.</p> <h3>Featured Solutions</h3> <h4>Rapid Non-Viral Gene Delivery</h4> <p>Speed up gene delivery with the transposon-based TcBuster™. This non-viral system enables transgenes up to 10 kb to be quickly generated and electroporated into target cells – a valuable tool for both proof-of-concept and manufacturing process development.</p> <p><a href="/services/gene-engineering-services">Find Out More About TcBuster</a></p> <h4>Reliably Quantify CAR+ Cells </h4> <p>Easily evaluate your percentage of successfully engineered cells with Fluorokines™ - fluorescent-labeled recombinant proteins. Directly stain, detect and quantify CAR expression on your cells with high specificity by flow cytometry.</p> <p><a href="/reagents/proteins/fluorokines-fluorescent-labeled-proteins">Learn More About Fluorokines</a></p> <h4>Cell Dispensing Made Easy</h4> <p>Dispense single cells and achieve optimal clonality with Namocell Single Cell Dispensers, enabling highly efficient and precise clone selection for cell engineering.</p> <p><a href="/instruments/single-cell-dispensers">Discover Namocell Single Cell Dispensers</a></p> <h4>Automated Protein Expression Quantification</h4> <p>Easily quantify protein expression and confirm knock-out following gene editing with Simple Western – the only fully automated Western blot system on the market. Simple Western instruments combine capillary electrophoresis with sensitive immunodetection, allowing you to quantify target proteins in 3 hours, using just 3uL of sample.</p> <p><a href="/instruments/simple-western">Learn About Simple Western</a></p> <h3>Resources</h3> <ul> <li><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/c-bio-204wt-1018609-cgti2022152-v5.pdf">Article: Accelerating Cell Therapy Discovery & Development with Non-Viral Gene Engineering</a></li> <li><a href="/resources/literature/lentiviral-vector-analysis-cell-and-gene-therapy-made-simple">Application Note: Lentiviral Vector Analysis for Cell and Gene Therapy Made Simple</a></li> <li><a href="https://www.namocell.com/resource-dr-perez-from-your-peers/" target="_blank">From Your Peers: Accelerate the Development of CRISPR Cell Line Engineering</a></li> </ul></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-immune-cell-expansion0" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Immune Cell Expansion</h5> </a> </div> <div class="collapse" id="expPara-immune-cell-expansion0" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><h2>Immune Cell Expansion</h2> <p>Obtaining robust cell expansion is important for downstream processing steps and meeting therapeutic dosing requirements. Scalability and lot consistency are the main challenges to overcome at this stage, and that’s why we provide high-quality bioreactors, media, and cytokines to help you generate large and consistent batches in every single run.</p> <h3>Featured Solutions</h3> <h4>Quality Cytokines & Growth Factors</h4> <p>Increase safety, ensure bioactivity, and minimize variability with high quality Animal-Free RUO and GMP proteins. We ensure lot-to-lot consistency in both purity and bioactivity with our robust quality management system.</p> <p><a href="/gmp-products/gmp-proteins">Browse Animal-Free and GMP Cytokines</a></p> <h4>Scale Up Your Process</h4> <p>Scale up and scale out with practicality, speed, and consistency via our ScaleReady partnership. G-Rex™ is a flexible, scalable gas-permeable cell culture bioreactor that delivers rapid cell expansion. G-Rex overcomes the limits of traditional plates and flasks with extended nutrient delivery and no added labor, pumps, mixing or shaking.</p> <p><a href="https://www.scaleready.com/product/g-rex/" target="_blank">Discover G-Rex at ScaleReady</a></p> <h4>Optimize Your Immune Cell Cultures</h4> <p>Ensure optimal consistency in supply, formulation, quality, and lot consistency with our ExCellerate™ media. With formulations optimized for T cell or NK cell cultures, these media are both serum- and xeno-free.</p> <p><a href="/reagents/cell-culture-reagents/excellerate">View Immune Cell Media</a></p> <h4>Monitoring Cell Expansion</h4> <p>Our immunoassay workflow solutions enable sensitive and specific cell expansion analysis, whilst conserving precious samples. Using Simple Western™ systems, you can combine protein size or charge separation with a quantitative immunoassay readout, producing accurate, reproducible, and highly specific analytical-grade protein expression potency assays.</p> <p><a href="/applications/immunoassays">Discover Immunoassay Solutions</a></p> <h3>Resources</h3> <ul> <li><a href="/resources/literature/simple-western-brochure">Brochure: Simple Western Product Guide</a></li> <li><a href="/gmp-products/ruo-to-gmp">Web: Transition to GMP for Cell Therapy Manufacturing</a></li> <li><a href="/gmp-products/gmp-capabilities">Web: GMP Manufacturing Capabilities</a></li> </ul></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-final-product-characterization4" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Final Product Characterization</h5> </a> </div> <div class="collapse" id="expPara-final-product-characterization4" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><h2>Final Product Characterization</h2> <p>Ensuring that your therapy meets all critical quality attributes (CQAs) requires assays and equipment with high specificity and sensitivity. Confirm that your finished product is functional, safe and consistent with customizable, flexible, and automated assay and analytics systems that provide you with the reliable, reproducible results you need.</p> <h3>Featured Solutions</h3> <h4>Detect CARs with High Specificity</h4> <p>Discover a full suite of high-specificity Flow Cytometry antibodies, as well as Fluorokines™, fluorescent-labeled recombinant proteins, that enable highly specific detection of cell markers, CARs, immune checkpoint receptors, and more by flow cytometry.</p> <p><a href="/applications/flow-cytometry">Discover Flow Cytometry Reagents</a></p> <h4>Automate Your Cytokine Release Assays</h4> <p>Ella™ is a benchtop automated ELISA platform that delivers consistent, reproducible data with no manual steps. With Ella, you can perform highly specific and reliable potency assays of your final products, allowing you to detect key markers of cytotoxic activity such as IFN-γ.</p> <p><a href="/research-areas/immune-cell-therapy/ifn-gamma-assay-qc-release-testing-ella">Ella IFN-gamma Assay for QC Release Testing</a></p> <h3>Resources</h3> <ul> <li><a href="https://www.rndsystems.com/resources/articles/fluorokine-app-note" target="_blank">Application Note: A Simple Flow Cytometry-Based Assay for Detecting CAR Expression Using Fluorokines</a></li> <li><a href="/resources/literature/ella-your-immunoassay-problem-solver">Brochure: Ella Product Guide</a></li> <li><a href="/resources/instrument-applications/simple-plex-cell-therapy">Web: Simple Plex Assays for Cell Therapy Research and Development</a></li> </ul></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-monitoring13" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Monitoring</h5> </a> </div> <div class="collapse" id="expPara-monitoring13" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><h2>Monitoring</h2><p>It’s crucial to monitor engineered cells after infusion to validate the efficacy and safety of your cell therapy. Our monitoring solutions can track diverse metrics like cell trafficking, persistence, and activation, cytokine release syndrome (CRS), and disease biomarkers, to enhance your patient outcomes.</p><h3>Featured Solutions</h3><h4>Verify Cell Therapy Delivery and Response with High Specificity</h4><p>RNAscope ISH assays enable spatial detection of cell therapies in preclinical tissues and biopsy samples. The single cell and single molecule sensitivity enables robust analysis of therapeutic biodistribution, safety and efficacy assessment. Combining RNAscope ISH with Immunohistochemistry/Immunofluorescence allows detection of immune cells and their activation states within the tissue by visualizing cytokine expression in therapeutic and endogenous immune cells.</p><p><a href="/reagents/rnascope-ish-technology">Learn More About RNAscope ISH Technology</a></p><h4>High-Throughput Monitoring of the Immune Response</h4><p>Our Luminex assays maximize multiplexing capacity and flexibility while maintaining assay specificity. These bead-based assays ensure robust and reproducible data, rapidly quantifying immune responses and cytokine release. Customize panels using the extensive mix-and-match menu, with up to 50 targets analyzed per sample. patient clinical immune monitoring</p><p><a href="/reagents/luminex-assays">Explore Luminex Assays</a></p><h4>Decoding Tumor Complexity</h4><p>By pairing the flexibility and specificity of Western blots with the analytical-grade quantitation of ELISA, Simple Western™ systems are ideal for shedding light on T-cell activation, exhaustion mechanisms, and the complexities of tumor microenvironments. For a more comprehensive understanding of cell heterogeneity, Single-Cell Westerns can analyze the tumor microenvironment at a single-cell level, revealing nuances in protein expression with unparalleled precision.</p><p><a href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions">Discover Simple Western</a></p><h3>Resources<br> </h3><ul><li><a href="https://resources.bio-techne.com/bio-techne-assets/images/resources/car-t-app-note-wr.pdf" target="_blank">Application Note: Preclinical CAR-T Cell Target Safety, Biodistribution, and Tumor Infiltration Analysis using the RNAscope ISH Assay</a></li><li><a href="/resources/literature/keeping-promise-of-immuno-oncology-simple-western-single-cell-western">Application Note: Keeping the Promise of Immuno-Oncology with Simple Western and Single-Cell Western</a></li><li><a href="https://acdbio.com/system/files_force/Cell_Therapy_Flyer.pdf?download=1">Flyer: T-Cell Therapy Trafficking and Activation Analysis Using RNAscope in situ Hybridization</a></li></ul></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Supporting Your Cell Therapy from Benchtop to Clinic</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Transitioning from research to the clinic can be challenging as you face more stringent qualification of raw materials. To help you move from RUO to GMP, we provide GMP cytokines and growth factors from our dedicated animal-free, GMP manufacturing facility. Each GMP product is directly comparable to our RUO animal-free version and is manufactured under ISO 9001 and 13485 Quality Management Systems, and to USP and European guidelines - so you can transition easily, with confidence in the supply and quality of your raw materials.</p><p>Our solutions can ease your transition from RUO to GMP:</p><ul><li>Scalable through commercialization</li><li>Large lot sizes mean less bridging</li><li>Full quality and regulatory support</li><li>Drug Master Files available</li></ul></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/gmp-products/ruo-to-gmp" class="btn btn-primary" type="link"> Streamline Your Transition to GMP </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/gmp-cell-therapy1050x700.png" alt="GMP Cell Therapy" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com/research-areas/immune-cell-therapy/scaleready" title="Learn More About ScaleReady" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/g-rex-cytokines-410x410-pink-fill.png" alt="G-Rex and GMP cytokines for transition to clinical manufacturing" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><h3> </h3><h3>ScaleReady™ Immune Cell Manufacturing Workflow</h3><p>Our ScaleReady partnership accelerates immune cell therapy manufacturing, enabling high throughput parallel processing within a small footprint – efficiently producing more with less.</p><p> </p></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/research-areas/immune-cell-therapy/scaleready" class="btn btn-primary" type="link"> Learn More About ScaleReady </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Custom Services for Immune Cell Therapy</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Can’t find what you’re looking for or have specific requirements? 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paragraph--view-mode--default card-para-default"> <h2>Immune Cell Therapy Resources</h2> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/t-cell-therapies-ebook" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/t-cell-based-therapies-thumbnail600x575.png" alt="T Cell Based Therapies Thumbnail" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/t-cell-therapies-ebook" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/t-cell-therapies-ebook" > eBook: T Cell-Based Therapies </a> </div> </div> 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<div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/lentiviral-transduction-non-viral-gene-delivery-car-t-manufacturing" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/tcb-vs-lenti-thumb-teaser.png" alt="Lentiviral vs. Non-Viral Gene Delivery for CAR-T Manufacturing App Note" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/lentiviral-transduction-non-viral-gene-delivery-car-t-manufacturing" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/lentiviral-transduction-non-viral-gene-delivery-car-t-manufacturing" > Application Note: Lentiviral vs. Non-Viral Gene Transfer </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/next-generation-analytical-solutions-cell-gene-therapy" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/next-gen-solutions-cgt-thumbnail600x575.png" alt="Next Generation Analytical Solutions for Cell & Gene Therapy Thumbnail" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/next-generation-analytical-solutions-cell-gene-therapy" > <h3 class="card_title_htag"></h3> </a> <div 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