CINXE.COM

<!DOCTYPE html> <html lang="en-US"> <head > <meta charset="UTF-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <meta name='robots' content='index, follow, max-image-preview:large, max-snippet:-1, max-video-preview:-1' />  <title>OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins</title> <meta name="description" content="Identifying and quantifying redox proteins uses biotin-tagging, iTRAQ-labeling, and higher energy collisional dissociation-collision-induced dissociation." /> <link rel="canonical" href="https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/" /> <meta property="og:locale" content="en_US" /> <meta property="og:type" content="article" /> <meta property="og:title" content="OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins" /> <meta property="og:description" content="Identifying and quantifying redox proteins uses biotin-tagging, iTRAQ-labeling, and higher energy collisional dissociation-collision-induced dissociation." /> <meta property="og:url" content="https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/" /> <meta property="og:site_name" content="Accelerating Proteomics" /> <meta property="article:publisher" content="https://www.facebook.com/thermofisher" /> <meta property="article:published_time" content="2014-03-28T11:00:15+00:00" /> <meta name="author" content="Melissa J. Mayer" /> <meta name="twitter:card" content="summary_large_image" /> <meta name="twitter:creator" content="@thermofisher" /> <meta name="twitter:site" content="@thermofisher" /> <meta name="twitter:label1" content="Written by" /> <meta name="twitter:data1" content="Melissa J. Mayer" /> <meta name="twitter:label2" content="Est. reading time" /> <meta name="twitter:data2" content="4 minutes" /> <script type="application/ld+json" class="yoast-schema-graph">{"@context":"https://schema.org","@graph":[{"@type":"WebPage","@id":"https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/","url":"https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/","name":"OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins","isPartOf":{"@id":"https://www.thermofisher.com/blog/proteomics/#website"},"datePublished":"2014-03-28T11:00:15+00:00","dateModified":"2014-03-28T11:00:15+00:00","author":{"@id":"https://www.thermofisher.com/blog/proteomics/#/schema/person/7344c200488dd2eefb46d830c8ac4dbb"},"description":"Identifying and quantifying redox proteins uses biotin-tagging, iTRAQ-labeling, and higher energy collisional dissociation-collision-induced dissociation.","breadcrumb":{"@id":"https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/"]}]},{"@type":"BreadcrumbList","@id":"https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.thermofisher.com/blog/proteomics/"},{"@type":"ListItem","position":2,"name":"OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins"}]},{"@type":"WebSite","@id":"https://www.thermofisher.com/blog/proteomics/#website","url":"https://www.thermofisher.com/blog/proteomics/","name":"Accelerating Proteomics","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.thermofisher.com/blog/proteomics/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https://www.thermofisher.com/blog/proteomics/#/schema/person/7344c200488dd2eefb46d830c8ac4dbb","name":"Melissa J. Mayer","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/proteomics/#/schema/person/image/","url":"https://secure.gravatar.com/avatar/6e214524310ece2814a29b7af5adaff9?s=96&d=mm&r=g","contentUrl":"https://secure.gravatar.com/avatar/6e214524310ece2814a29b7af5adaff9?s=96&d=mm&r=g","caption":"Melissa J. Mayer"},"url":"https://www.thermofisher.com/blog/author/melissaj-mayer/"}]}</script>  <link rel='dns-prefetch' href='//www.thermofisher.com' /> <link rel='dns-prefetch' href='//use.fontawesome.com' /> <link rel="alternate" type="application/rss+xml" title="Accelerating Proteomics » Feed" href="https://www.thermofisher.com/blog/proteomics/feed/" /> <link rel="alternate" type="application/rss+xml" title="Accelerating Proteomics » Comments Feed" href="https://www.thermofisher.com/blog/proteomics/comments/feed/" /> <link rel="alternate" type="application/rss+xml" title="Accelerating Proteomics » OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins Comments Feed" href="https://www.thermofisher.com/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/feed/" />  <script src="https://cdn.optimizely.com/js/8156647427.js"></script>  <script> (function(h,o,t,j,a,r){ h.hj=h.hj||function(){(h.hj.q=h.hj.q||[]).push(arguments)}; h._hjSettings={hjid:373708,hjsv:5}; a=o.getElementsByTagName('head')[0]; r=o.createElement('script');r.async=1; r.src=t+h._hjSettings.hjid+j+h._hjSettings.hjsv; a.appendChild(r); })(window,document,'//static.hotjar.com/c/hotjar-','.js?sv='); </script>  <script src="//www.googletagmanager.com/gtag/js?id=G-RHDLB4CBWB" data-cfasync="false" data-wpfc-render="false" type="text/javascript" async></script> <script data-cfasync="false" data-wpfc-render="false" type="text/javascript"> var mi_version = '9.2.0'; var mi_track_user = true; var mi_no_track_reason = ''; var MonsterInsightsDefaultLocations = {"page_location":"https:\/\/admin.acceleratingscience.com\/proteomics\/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins\/"}; if ( typeof MonsterInsightsPrivacyGuardFilter === 'function' ) { var MonsterInsightsLocations = (typeof MonsterInsightsExcludeQuery === 'object') ? MonsterInsightsPrivacyGuardFilter( MonsterInsightsExcludeQuery ) : MonsterInsightsPrivacyGuardFilter( MonsterInsightsDefaultLocations ); } else { var MonsterInsightsLocations = (typeof MonsterInsightsExcludeQuery === 'object') ? MonsterInsightsExcludeQuery : MonsterInsightsDefaultLocations; } var disableStrs = [ 'ga-disable-G-RHDLB4CBWB', ]; /* Function to detect opted out users */ function __gtagTrackerIsOptedOut() { for (var index = 0; index < disableStrs.length; index++) { if (document.cookie.indexOf(disableStrs[index] + '=true') > -1) { return true; } } return false; } /* Disable tracking if the opt-out cookie exists. */ if (__gtagTrackerIsOptedOut()) { for (var index = 0; index < disableStrs.length; index++) { window[disableStrs[index]] = true; } } /* Opt-out function */ function __gtagTrackerOptout() { for (var index = 0; index < disableStrs.length; index++) { document.cookie = disableStrs[index] + '=true; expires=Thu, 31 Dec 2099 23:59:59 UTC; path=/'; window[disableStrs[index]] = true; } } if ('undefined' === typeof gaOptout) { function gaOptout() { __gtagTrackerOptout(); } } window.dataLayer = window.dataLayer || []; window.MonsterInsightsDualTracker = { helpers: {}, trackers: {}, }; if (mi_track_user) { function __gtagDataLayer() { dataLayer.push(arguments); } function __gtagTracker(type, name, parameters) { if (!parameters) { parameters = {}; } if (parameters.send_to) { __gtagDataLayer.apply(null, arguments); return; } if (type === 'event') { parameters.send_to = monsterinsights_frontend.v4_id; var hookName = name; if (typeof parameters['event_category'] !== 'undefined') { hookName = parameters['event_category'] + ':' + name; } if (typeof MonsterInsightsDualTracker.trackers[hookName] !== 'undefined') { MonsterInsightsDualTracker.trackers[hookName](parameters); } else { __gtagDataLayer('event', name, parameters); } } else { __gtagDataLayer.apply(null, arguments); } } __gtagTracker('js', new Date()); __gtagTracker('set', { 'developer_id.dZGIzZG': true, }); if ( MonsterInsightsLocations.page_location ) { __gtagTracker('set', MonsterInsightsLocations); } __gtagTracker('config', 'G-RHDLB4CBWB', {"use_amp_client_id":"true","forceSSL":"true","author":"Melissa Mayer","category":"non-human-proteomics","published_at":"2014-03-28T07:00:15+00:00","tags":"oxidative stress,OxiTRAQ,Redox"} ); window.gtag = __gtagTracker; (function () { /* https://developers.google.com/analytics/devguides/collection/analyticsjs/ */ /* ga and __gaTracker compatibility shim. */ var noopfn = function () { return null; }; var newtracker = function () { return new Tracker(); }; var Tracker = function () { return null; }; var p = Tracker.prototype; p.get = noopfn; p.set = noopfn; p.send = function () { var args = Array.prototype.slice.call(arguments); args.unshift('send'); __gaTracker.apply(null, args); }; var __gaTracker = function () { var len = arguments.length; if (len === 0) { return; } var f = arguments[len - 1]; if (typeof f !== 'object' || f === null || typeof f.hitCallback !== 'function') { if ('send' === arguments[0]) { var hitConverted, hitObject = false, action; if ('event' === arguments[1]) { if ('undefined' !== typeof arguments[3]) { hitObject = { 'eventAction': arguments[3], 'eventCategory': arguments[2], 'eventLabel': arguments[4], 'value': arguments[5] ? arguments[5] : 1, } } } if ('pageview' === arguments[1]) { if ('undefined' !== typeof arguments[2]) { hitObject = { 'eventAction': 'page_view', 'page_path': arguments[2], } } } if (typeof arguments[2] === 'object') { hitObject = arguments[2]; } if (typeof arguments[5] === 'object') { Object.assign(hitObject, arguments[5]); } if ('undefined' !== typeof arguments[1].hitType) { hitObject = arguments[1]; if ('pageview' === hitObject.hitType) { hitObject.eventAction = 'page_view'; } } if (hitObject) { action = 'timing' === arguments[1].hitType ? 'timing_complete' : hitObject.eventAction; hitConverted = mapArgs(hitObject); __gtagTracker('event', action, hitConverted); } } return; } function mapArgs(args) { var arg, hit = {}; var gaMap = { 'eventCategory': 'event_category', 'eventAction': 'event_action', 'eventLabel': 'event_label', 'eventValue': 'event_value', 'nonInteraction': 'non_interaction', 'timingCategory': 'event_category', 'timingVar': 'name', 'timingValue': 'value', 'timingLabel': 'event_label', 'page': 'page_path', 'location': 'page_location', 'title': 'page_title', 'referrer' : 'page_referrer', }; for (arg in args) { if (!(!args.hasOwnProperty(arg) || !gaMap.hasOwnProperty(arg))) { hit[gaMap[arg]] = args[arg]; } else { hit[arg] = args[arg]; } } return hit; } try { f.hitCallback(); } catch (ex) { } }; __gaTracker.create = newtracker; __gaTracker.getByName = newtracker; __gaTracker.getAll = function () { return []; }; __gaTracker.remove = noopfn; __gaTracker.loaded = true; window['__gaTracker'] = __gaTracker; })(); } else { console.log(""); (function () { function __gtagTracker() { return null; } window['__gtagTracker'] = __gtagTracker; window['gtag'] = __gtagTracker; })(); } </script>  <link rel='stylesheet' id='jetpack_related-posts-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/jetpack/modules/related-posts/related-posts.css?ver=20240116' type='text/css' media='all' /> <link rel='stylesheet' id='accelerating-science-blogs-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/themes/accelerating-science-blogs/style.css?ver=1.2.9.3' type='text/css' media='all' /> <link rel='stylesheet' id='wp-block-library-css' href='https://www.thermofisher.com/blog//wp-includes/css/dist/block-library/style.min.css?ver=6.6.1' type='text/css' media='all' /> <link rel='stylesheet' id='mediaelement-css' href='https://www.thermofisher.com/blog//wp-includes/js/mediaelement/mediaelementplayer-legacy.min.css?ver=4.2.17' type='text/css' media='all' /> <link rel='stylesheet' id='wp-mediaelement-css' href='https://www.thermofisher.com/blog//wp-includes/js/mediaelement/wp-mediaelement.min.css?ver=6.6.1' type='text/css' media='all' /> <style id='jetpack-sharing-buttons-style-inline-css' type='text/css'> .jetpack-sharing-buttons__services-list{display:flex;flex-direction:row;flex-wrap:wrap;gap:0;list-style-type:none;margin:5px;padding:0}.jetpack-sharing-buttons__services-list.has-small-icon-size{font-size:12px}.jetpack-sharing-buttons__services-list.has-normal-icon-size{font-size:16px}.jetpack-sharing-buttons__services-list.has-large-icon-size{font-size:24px}.jetpack-sharing-buttons__services-list.has-huge-icon-size{font-size:36px}@media print{.jetpack-sharing-buttons__services-list{display:none!important}}.editor-styles-wrapper .wp-block-jetpack-sharing-buttons{gap:0;padding-inline-start:0}ul.jetpack-sharing-buttons__services-list.has-background{padding:1.25em 2.375em} </style> <style id='classic-theme-styles-inline-css' type='text/css'> /*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} </style> <style id='global-styles-inline-css' type='text/css'> :root{--wp--preset--aspect-ratio--square: 1;--wp--preset--aspect-ratio--4-3: 4/3;--wp--preset--aspect-ratio--3-4: 3/4;--wp--preset--aspect-ratio--3-2: 3/2;--wp--preset--aspect-ratio--2-3: 2/3;--wp--preset--aspect-ratio--16-9: 16/9;--wp--preset--aspect-ratio--9-16: 9/16;--wp--preset--color--black: #000000;--wp--preset--color--cyan-bluish-gray: #abb8c3;--wp--preset--color--white: #ffffff;--wp--preset--color--pale-pink: #f78da7;--wp--preset--color--vivid-red: #cf2e2e;--wp--preset--color--luminous-vivid-orange: #ff6900;--wp--preset--color--luminous-vivid-amber: #fcb900;--wp--preset--color--light-green-cyan: #7bdcb5;--wp--preset--color--vivid-green-cyan: #00d084;--wp--preset--color--pale-cyan-blue: #8ed1fc;--wp--preset--color--vivid-cyan-blue: #0693e3;--wp--preset--color--vivid-purple: #9b51e0;--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple: linear-gradient(135deg,rgba(6,147,227,1) 0%,rgb(155,81,224) 100%);--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan: linear-gradient(135deg,rgb(122,220,180) 0%,rgb(0,208,130) 100%);--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange: linear-gradient(135deg,rgba(252,185,0,1) 0%,rgba(255,105,0,1) 100%);--wp--preset--gradient--luminous-vivid-orange-to-vivid-red: linear-gradient(135deg,rgba(255,105,0,1) 0%,rgb(207,46,46) 100%);--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray: linear-gradient(135deg,rgb(238,238,238) 0%,rgb(169,184,195) 100%);--wp--preset--gradient--cool-to-warm-spectrum: linear-gradient(135deg,rgb(74,234,220) 0%,rgb(151,120,209) 20%,rgb(207,42,186) 40%,rgb(238,44,130) 60%,rgb(251,105,98) 80%,rgb(254,248,76) 100%);--wp--preset--gradient--blush-light-purple: linear-gradient(135deg,rgb(255,206,236) 0%,rgb(152,150,240) 100%);--wp--preset--gradient--blush-bordeaux: linear-gradient(135deg,rgb(254,205,165) 0%,rgb(254,45,45) 50%,rgb(107,0,62) 100%);--wp--preset--gradient--luminous-dusk: linear-gradient(135deg,rgb(255,203,112) 0%,rgb(199,81,192) 50%,rgb(65,88,208) 100%);--wp--preset--gradient--pale-ocean: linear-gradient(135deg,rgb(255,245,203) 0%,rgb(182,227,212) 50%,rgb(51,167,181) 100%);--wp--preset--gradient--electric-grass: linear-gradient(135deg,rgb(202,248,128) 0%,rgb(113,206,126) 100%);--wp--preset--gradient--midnight: linear-gradient(135deg,rgb(2,3,129) 0%,rgb(40,116,252) 100%);--wp--preset--font-size--small: 13px;--wp--preset--font-size--medium: 20px;--wp--preset--font-size--large: 36px;--wp--preset--font-size--x-large: 42px;--wp--preset--spacing--20: 0.44rem;--wp--preset--spacing--30: 0.67rem;--wp--preset--spacing--40: 1rem;--wp--preset--spacing--50: 1.5rem;--wp--preset--spacing--60: 2.25rem;--wp--preset--spacing--70: 3.38rem;--wp--preset--spacing--80: 5.06rem;--wp--preset--shadow--natural: 6px 6px 9px rgba(0, 0, 0, 0.2);--wp--preset--shadow--deep: 12px 12px 50px rgba(0, 0, 0, 0.4);--wp--preset--shadow--sharp: 6px 6px 0px rgba(0, 0, 0, 0.2);--wp--preset--shadow--outlined: 6px 6px 0px -3px rgba(255, 255, 255, 1), 6px 6px rgba(0, 0, 0, 1);--wp--preset--shadow--crisp: 6px 6px 0px rgba(0, 0, 0, 1);}:where(.is-layout-flex){gap: 0.5em;}:where(.is-layout-grid){gap: 0.5em;}body .is-layout-flex{display: flex;}.is-layout-flex{flex-wrap: wrap;align-items: center;}.is-layout-flex > :is(*, div){margin: 0;}body .is-layout-grid{display: grid;}.is-layout-grid > :is(*, div){margin: 0;}:where(.wp-block-columns.is-layout-flex){gap: 2em;}:where(.wp-block-columns.is-layout-grid){gap: 2em;}:where(.wp-block-post-template.is-layout-flex){gap: 1.25em;}:where(.wp-block-post-template.is-layout-grid){gap: 1.25em;}.has-black-color{color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-color{color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-color{color: var(--wp--preset--color--white) !important;}.has-pale-pink-color{color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-color{color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-color{color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-color{color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-color{color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-color{color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-color{color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-color{color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-color{color: var(--wp--preset--color--vivid-purple) !important;}.has-black-background-color{background-color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-background-color{background-color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-background-color{background-color: var(--wp--preset--color--white) !important;}.has-pale-pink-background-color{background-color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-background-color{background-color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-background-color{background-color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-background-color{background-color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-background-color{background-color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-background-color{background-color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-background-color{background-color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-background-color{background-color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-background-color{background-color: var(--wp--preset--color--vivid-purple) !important;}.has-black-border-color{border-color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-border-color{border-color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-border-color{border-color: var(--wp--preset--color--white) !important;}.has-pale-pink-border-color{border-color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-border-color{border-color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-border-color{border-color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-border-color{border-color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-border-color{border-color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-border-color{border-color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-border-color{border-color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-border-color{border-color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-border-color{border-color: var(--wp--preset--color--vivid-purple) !important;}.has-vivid-cyan-blue-to-vivid-purple-gradient-background{background: var(--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple) !important;}.has-light-green-cyan-to-vivid-green-cyan-gradient-background{background: var(--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan) !important;}.has-luminous-vivid-amber-to-luminous-vivid-orange-gradient-background{background: var(--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange) !important;}.has-luminous-vivid-orange-to-vivid-red-gradient-background{background: var(--wp--preset--gradient--luminous-vivid-orange-to-vivid-red) !important;}.has-very-light-gray-to-cyan-bluish-gray-gradient-background{background: var(--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray) !important;}.has-cool-to-warm-spectrum-gradient-background{background: var(--wp--preset--gradient--cool-to-warm-spectrum) !important;}.has-blush-light-purple-gradient-background{background: var(--wp--preset--gradient--blush-light-purple) !important;}.has-blush-bordeaux-gradient-background{background: var(--wp--preset--gradient--blush-bordeaux) !important;}.has-luminous-dusk-gradient-background{background: var(--wp--preset--gradient--luminous-dusk) !important;}.has-pale-ocean-gradient-background{background: var(--wp--preset--gradient--pale-ocean) !important;}.has-electric-grass-gradient-background{background: var(--wp--preset--gradient--electric-grass) !important;}.has-midnight-gradient-background{background: var(--wp--preset--gradient--midnight) !important;}.has-small-font-size{font-size: var(--wp--preset--font-size--small) !important;}.has-medium-font-size{font-size: var(--wp--preset--font-size--medium) !important;}.has-large-font-size{font-size: var(--wp--preset--font-size--large) !important;}.has-x-large-font-size{font-size: var(--wp--preset--font-size--x-large) !important;} :where(.wp-block-post-template.is-layout-flex){gap: 1.25em;}:where(.wp-block-post-template.is-layout-grid){gap: 1.25em;} :where(.wp-block-columns.is-layout-flex){gap: 2em;}:where(.wp-block-columns.is-layout-grid){gap: 2em;} :root :where(.wp-block-pullquote){font-size: 1.5em;line-height: 1.6;} </style> <link rel='stylesheet' id='mashsb-styles-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/mashsharer/assets/css/mashsb.min.css?ver=4.0.47' type='text/css' media='all' /> <style id='mashsb-styles-inline-css' type='text/css'> .mashsb-count {color:#555759;}[class^="mashicon-"] .text, [class*=" mashicon-"] .text{ text-indent: -9999px !important; line-height: 0px; display: block; } [class^="mashicon-"] .text:after, [class*=" mashicon-"] .text:after { content: "" !important; text-indent: 0; font-size:13px; display: block !important; } [class^="mashicon-"], [class*=" mashicon-"] { width:25%; text-align: center !important; } [class^="mashicon-"] .icon:before, [class*=" mashicon-"] .icon:before { float:none; margin-right: 0; } .mashsb-buttons a{ margin-right: 3px; margin-bottom:3px; min-width: 0; width: 41px; } .onoffswitch, .onoffswitch-inner:before, .onoffswitch-inner:after .onoffswitch2, .onoffswitch2-inner:before, .onoffswitch2-inner:after { margin-right: 0px; width: 41px; line-height: 41px; } </style> <link rel='stylesheet' id='mashnet-styles-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/mashshare-networks/assets/css/mashnet.min.css?ver=2.5.3' type='text/css' media='all' /> <link rel='stylesheet' id='mashsas-styles-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/mashshare-select-and-share/assets/css/mashsas.min.css?ver=1.0.5' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_reset_css-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/legacy/css/formreset.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_formsmain_css-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/legacy/css/formsmain.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_ready_class_css-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/legacy/css/readyclass.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_browsers_css-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/legacy/css/browsers.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='globals-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/themes/accelerating-science-blogs/global-style.css?ver=1.2.9.3' type='text/css' media='all' /> <link rel='stylesheet' id='bfa-font-awesome-css' href='https://use.fontawesome.com/releases/v5.15.4/css/all.css?ver=2.0.3' type='text/css' media='all' /> <link rel='stylesheet' id='bfa-font-awesome-v4-shim-css' href='https://use.fontawesome.com/releases/v5.15.4/css/v4-shims.css?ver=2.0.3' type='text/css' media='all' /> <style id='bfa-font-awesome-v4-shim-inline-css' type='text/css'> @font-face { font-family: 'FontAwesome'; src: url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.eot'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.eot?#iefix') format('embedded-opentype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.woff2') format('woff2'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.woff') format('woff'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.ttf') format('truetype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-brands-400.svg#fontawesome') format('svg'); } @font-face { font-family: 'FontAwesome'; src: url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.eot'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.eot?#iefix') format('embedded-opentype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.woff2') format('woff2'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.woff') format('woff'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.ttf') format('truetype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-solid-900.svg#fontawesome') format('svg'); } @font-face { font-family: 'FontAwesome'; src: url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.eot'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.eot?#iefix') format('embedded-opentype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.woff2') format('woff2'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.woff') format('woff'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.ttf') format('truetype'), url('https://use.fontawesome.com/releases/v5.15.4/webfonts/fa-regular-400.svg#fontawesome') format('svg'); unicode-range: U+F004-F005,U+F007,U+F017,U+F022,U+F024,U+F02E,U+F03E,U+F044,U+F057-F059,U+F06E,U+F070,U+F075,U+F07B-F07C,U+F080,U+F086,U+F089,U+F094,U+F09D,U+F0A0,U+F0A4-F0A7,U+F0C5,U+F0C7-F0C8,U+F0E0,U+F0EB,U+F0F3,U+F0F8,U+F0FE,U+F111,U+F118-F11A,U+F11C,U+F133,U+F144,U+F146,U+F14A,U+F14D-F14E,U+F150-F152,U+F15B-F15C,U+F164-F165,U+F185-F186,U+F191-F192,U+F1AD,U+F1C1-F1C9,U+F1CD,U+F1D8,U+F1E3,U+F1EA,U+F1F6,U+F1F9,U+F20A,U+F247-F249,U+F24D,U+F254-F25B,U+F25D,U+F267,U+F271-F274,U+F279,U+F28B,U+F28D,U+F2B5-F2B6,U+F2B9,U+F2BB,U+F2BD,U+F2C1-F2C2,U+F2D0,U+F2D2,U+F2DC,U+F2ED,U+F328,U+F358-F35B,U+F3A5,U+F3D1,U+F410,U+F4AD; } </style> <link rel='stylesheet' id='boxzilla-css' href='https://www.thermofisher.com/blog/proteomics/wp-content/plugins/boxzilla/assets/css/styles.css?ver=3.3.1' type='text/css' media='all' /> <script type="text/javascript" id="jetpack_related-posts-js-extra"> /* <![CDATA[ */ var related_posts_js_options = {"post_heading":"h4"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/jetpack/_inc/build/related-posts/related-posts.min.js?ver=20240116" id="jetpack_related-posts-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/google-analytics-premium/assets/js/frontend-gtag.min.js?ver=9.2.0" id="monsterinsights-frontend-script-js" async="async" data-wp-strategy="async"></script> <script data-cfasync="false" data-wpfc-render="false" type="text/javascript" id='monsterinsights-frontend-script-js-extra'>/* <![CDATA[ */ var monsterinsights_frontend = {"js_events_tracking":"true","download_extensions":"doc,pdf,ppt,zip,xls,docx,pptx,xlsx","inbound_paths":"[]","home_url":"https:\/\/admin.acceleratingscience.com\/proteomics","hash_tracking":"false","v4_id":"G-RHDLB4CBWB"};/* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/jquery.min.js?ver=3.7.1" id="jquery-core-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/jquery-migrate.min.js?ver=3.4.1" id="jquery-migrate-js"></script> <script type="text/javascript" id="mashsb-js-extra"> /* <![CDATA[ */ var mashsb = {"shares":"10","round_shares":"1","animate_shares":"0","dynamic_buttons":"0","share_url":"https:\/\/www.thermofisher.com\/blog\/proteomics\/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins\/","title":"OxiTRAQ%3A+A+Novel+Approach+to+Identify+Redox-Sensitive+Proteins","image":null,"desc":"When biological stresses induce the overproduction of reactive oxygen within the cell, redox-sensitive proteins may undergo oxidative modifications. The result may be oxidative damage to cellular components, including lipids, proteins and DNA. The modifications may \u2026","hashtag":"thermofisher","subscribe":"content","subscribe_url":"","activestatus":"1","singular":"1","twitter_popup":"1","refresh":"1","nonce":"01c3793ea9","postid":"2476","servertime":"1732381502","ajaxurl":"https:\/\/www.thermofisher.com\/blog\/wp-admin\/admin-ajax.php"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/mashsharer/assets/js/mashsb.min.js?ver=4.0.47" id="mashsb-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/mashshare-google-analytics/assets/js/mashga.min.js?ver=1.0.9" id="mashga-js"></script> <script type="text/javascript" id="mashnet-js-extra"> /* <![CDATA[ */ var mashnet = {"body":"Check out this article I just read:","subject":"Check out this article from Accelerating Science","pinterest_select":"1","pinterest_image":"","pinterest_desc":"When biological stresses induce the overproduction of reactive oxygen within the cell, redox-sensitive proteins may undergo oxidative modifications. The result may be oxidative damage to cellular components, including lipids, proteins and DNA. The modifications may \u2026"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/mashshare-networks/assets/js/mashnet.min.js?ver=2.5.3" id="mashnet-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/underscore.min.js?ver=1.13.4" id="underscore-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/backbone.min.js?ver=1.5.0" id="backbone-js"></script> <script type="text/javascript" id="wp-api-js-js-extra"> /* <![CDATA[ */ var WP_API_Settings = {"root":"https:\/\/admin.acceleratingscience.com\/proteomics","nonce":"ba522f8159"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/wp-search-live/public/assets/js/util--wp-api.js?ver=0.9" id="wp-api-js-js"></script> <script type="text/javascript" id="mashsas-js-extra"> /* <![CDATA[ */ var mashsas = {"minchars":"10","appid":"1603510369972799","facebook":"1","twitter":"1","mail":"1"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/mashshare-select-and-share/assets/js/mashsas.min.js?ver=1.0.5" id="mashsas-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/js/jquery.json.min.js?ver=2.8.17" id="gform_json-js"></script> <script type="text/javascript" id="gform_gravityforms-js-extra"> /* <![CDATA[ */ var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gform_i18n = {"datepicker":{"days":{"monday":"Mo","tuesday":"Tu","wednesday":"We","thursday":"Th","friday":"Fr","saturday":"Sa","sunday":"Su"},"months":{"january":"January","february":"February","march":"March","april":"April","may":"May","june":"June","july":"July","august":"August","september":"September","october":"October","november":"November","december":"December"},"firstDay":1,"iconText":"Select date"}}; var gf_legacy_multi = {"1":"1","2":"1"}; var gform_gravityforms = {"strings":{"invalid_file_extension":"This type of file is not allowed. Must be one of the following:","delete_file":"Delete this file","in_progress":"in progress","file_exceeds_limit":"File exceeds size limit","illegal_extension":"This type of file is not allowed.","max_reached":"Maximum number of files reached","unknown_error":"There was a problem while saving the file on the server","currently_uploading":"Please wait for the uploading to complete","cancel":"Cancel","cancel_upload":"Cancel this upload","cancelled":"Cancelled"},"vars":{"images_url":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms\/images"}}; /* ]]> */ </script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/js/gravityforms.min.js?ver=2.8.17" id="gform_gravityforms-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/js/chosen.jquery.min.js?ver=2.8.17" id="gform_chosen-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/assets/js/dist/utils.min.js?ver=50c7bea9c2320e16728e44ae9fde5f26" id="gform_gravityforms_utils-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/themes/accelerating-science-blogs/js/single-scripts.js?ver=1.2.9.3" id="single-scripts-js"></script> <link rel="https://api.w.org/" href="https://www.thermofisher.com/blog/proteomics/wp-json/" /><link rel="alternate" title="JSON" type="application/json" href="https://www.thermofisher.com/blog/proteomics/wp-json/wp/v2/posts/2476" /><link rel="EditURI" type="application/rsd+xml" title="RSD" href="https://www.thermofisher.com/blog/xmlrpc.php?rsd" /> <link rel='shortlink' href='https://www.thermofisher.com/blog/proteomics/?p=2476' /> <link rel="alternate" title="oEmbed (JSON)" type="application/json+oembed" href="https://www.thermofisher.com/blog/proteomics/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fproteomics%2Foxitraq-a-novel-approach-to-identify-redox-sensitive-proteins%2F" /> <link rel="alternate" title="oEmbed (XML)" type="text/xml+oembed" href="https://www.thermofisher.com/blog/proteomics/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fproteomics%2Foxitraq-a-novel-approach-to-identify-redox-sensitive-proteins%2F&format=xml" /> <meta property="fb:pages" content="258357757657262" />  <link rel="icon" href="https://www.thermofisher.com/blog/proteomics/wp-content/themes/accelerating-science-blogs/images/favicon.ico" />  <script> !function(f,b,e,v,n,t,s){if(f.fbq)return;n=f.fbq=function(){n.callMethod? n.callMethod.apply(n,arguments):n.queue.push(arguments)};if(!f._fbq)f._fbq=n; n.push=n;n.loaded=!0;n.version='2.0';n.queue=[];t=b.createElement(e);t.async=!0; t.src=v;s=b.getElementsByTagName(e)[0];s.parentNode.insertBefore(t,s)}(window, document,'script','https://connect.facebook.net/en_US/fbevents.js'); fbq('init', '305572349637096'); fbq('track', "PageView");</script> <noscript><img height="1" width="1" style="display:none" src="https://www.facebook.com/tr?id=305572349637096&ev=PageView&noscript=1" /></noscript>  <script type="application/ld+json"> {"@context":"http:\/\/schema.org","@type":"BlogPosting","mainEntityofPage":{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/proteomics\/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins\/"},"headline":"OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins","datePublished":"03\/28\/2014","dateModified":"03\/28\/2014","publisher":{"@type":"Organization","name":"Thermo Fisher Scientific","URL":"https:\/\/www.thermofisher.com\/us\/en\/home.html","logo":{"@type":"ImageObject","url":"https:\/\/www.thermofisher.com\/content\/dam\/LifeTech\/Images\/Header\/logo-color.png"}},"keywords":"oxidative stress, OxiTRAQ, Redox, ","author":{"@type":"Person","name":"Melissa J. Mayer","image":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/uploads\/2016\/04\/Melissa-80x80.jpg"}} </script> <script type="text/javascript" src="https://www.thermofisher.com/etc/designs/lifetech/clientlibs/digitaldata.js" defer="defer"></script> <script id="tagmanager" type="text/javascript" src='https://assets.adobedtm.com/launch-ENeecb77f1160a4928a492ff040f7110c7.min.js' defer="defer"></script> <script> (function($) { $(document).ready(function() { digitalData.setPageType('blog'); }) })(jQuery); </script> </head> <body class="post-template-default single single-post postid-2476 single-format-standard header-image full-width-content genesis-breadcrumbs-visible"><div class="site-container"><header class="site-header"><div class="wrap"><div class="title-area"><p class="site-title"><a href="https://www.thermofisher.com/" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></p></div><div class="widget-area header-widget-area"><section id="nav_menu-2" class="widget widget_nav_menu"><div class="widget-wrap"><nav class="nav-header"><ul id="menu-main-menu" class="menu genesis-nav-menu"><li id="menu-item-7952" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-7952"><a><span >Categories</span></a> <ul class="sub-menu"> <li id="menu-item-7959" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-7959"><a href="https://www.thermofisher.com/blog/materials/"><span >Advancing Materials</span></a></li> <li id="menu-item-7958" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-7958"><a href="https://www.thermofisher.com/blog/mining/"><span >Advancing Mining</span></a></li> <li id="menu-item-9337" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9337"><a href="https://www.thermofisher.com/blog/analyteguru/"><span >AnalyteGuru</span></a></li> <li id="menu-item-7957" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-7957"><a href="https://www.thermofisher.com/blog/metals/"><span >Analyzing Metals</span></a></li> <li id="menu-item-9312" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9312"><a href="https://www.thermofisher.com/blog/ask-a-scientist/"><span >Ask a Scientist</span></a></li> <li id="menu-item-7953" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-7953"><a href="https://www.thermofisher.com/blog/behindthebench/"><span >Behind the Bench</span></a></li> <li id="menu-item-9335" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9335"><a href="https://www.thermofisher.com/blog/biotechnology/"><span >Biotech at Scale</span></a></li> <li id="menu-item-9318" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9318"><a href="https://www.thermofisher.com/blog/clinical-conversations/"><span >Clinical Conversations</span></a></li> <li id="menu-item-7956" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-7956"><a href="https://www.thermofisher.com/blog/food/"><span >Examining Food</span></a></li> <li id="menu-item-9287" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9287"><a href="https://www.thermofisher.com/blog/identifying-threats/"><span >Identifying Threats</span></a></li> <li id="menu-item-9324" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9324"><a href="https://www.thermofisher.com/blog/semiconductors/"><span >Illuminating Semiconductors</span></a></li> <li id="menu-item-9325" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9325"><a href="https://www.thermofisher.com/blog/atomic-resolution/"><span >Life in Atomic Resolution</span></a></li> <li id="menu-item-9284" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9284"><a href="https://www.thermofisher.com/blog/life-in-the-lab/"><span >Life in the Lab</span></a></li> <li id="menu-item-9319" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9319"><a href="https://www.thermofisher.com/blog/oempowered/"><span >OEMpowered</span></a></li> <li id="menu-item-9308" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-9308"><a href="https://www.thermofisher.com/blog/connectedlab/"><span >The Connected Lab</span></a></li> </ul> </li> <li id="menu-item-7961" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-7961"><a href="https://www.thermofisher.com/blog/about-us/"><span >About Us</span></a></li> <li id="menu-item-7962" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-7962"><a href="https://www.thermofisher.com/blog/contact-us/?site=proteomics"><span >Contact</span></a></li> </ul></nav></div></section> </div><div class="breadcrumb"><span class="breadcrumb-link-wrap"><a href="https://www.thermofisher.com/blog/?">Accelerating Science</a></span><span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/proteomics/"><span class="breadcrumb-link-text-wrap">Accelerating Proteomics</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/proteomics/non-human-proteomics/"><span class="breadcrumb-link-text-wrap">Non-Human Proteomics</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-2476 post type-post status-publish format-standard category-non-human-proteomics tag-oxidative-stress tag-oxitraq tag-redox entry" aria-label="OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins"><header class="entry-header"><h1 class="entry-title">OxiTRAQ: A Novel Approach to Identify Redox-Sensitive Proteins</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/melissaj-mayer/" title="Posts by Melissa J. Mayer" rel="author">Melissa J. Mayer</a> </span> <span class="post-info-date"> 03.28.2014 </span> </header><div class="entry-content"><p><span>When biological stresses induce the overproduction of reactive oxygen within the cell, redox-<span>sensitive proteins </span>may undergo oxidative modifications. The result may be oxidative damage to cellular components, including lipids, proteins and DNA. <span>The modifications may also play a role in regulating signal transduction pathways and gene expression</span>.<sup>1</sup> F</span>or many plants, including <em>Arabidopsis</em> species, the thiol group of cysteine residues is especially prone to stress-triggered oxidation and reduction.<sup><span style="font-size: xx-small">2,3</span></sup></p> <p>Researchers have relied upon fluorescent labeling and gel electrophoresis for the identification of most redox-sensitive proteins; however, low resolution and inadequate sensitivity render this approach ineffective for the identification of low-abundance proteins.<sup><span style="font-size: xx-small">4.5</span></sup> Other approaches, including tandem mass tagging (TMT) and isotope coded affinity tagging (ICAT), couple isotope labeling with mass spectrometry (MS). The expense associated with these methods, however, limits their applicability for whole-proteome analysis.</p> <p>In this study, Liu et al. (2013) describe OxiTRAQ, a high-throughput quantitative approach in which biotin-tagged, iTRAQ-labeled peptides are analyzed with parallel higher energy collisional dissociation–collision-induced dissociation (HCD-CID) on an LTQ-Orbitrap mass spectrometer (Thermo Scientific).<sup>6</sup> Using this technology, the researchers isolated 195 cysteine-containing peptides from 179 oxidatively modified <em>Arabidopsis</em> proteins, including 151 redox-sensitive proteins that had not been previously identified using other methods.</p> <p>To perform OxiTRAQ, Liu et al. used hydrogen peroxide to induce oxidative stress in <em>Arabidopsis</em> cells prior to extracting proteins in the presence of free thiol-blocking N-ethylmaleimide (NEM). They then reduced reversibly oxidized thiols (including disulfide bonds, sulfenic acid, and S-nitrosylated thiols) with dithiothreitol (DTT) before tagging with biotin-HPDP. After trypsin digestion and affinity purification, they cleaved the disulfide bonds between the tags and the cysteine residues and eluted the peptides for identification by MS. Following HCD-CID, the researchers analyzed the mass spectral data with Proteome Discoverer Software (Thermo Scientific) and verified their results using multiple reaction monitoring (MRM).</p> <p>In two identical MS runs, the researchers identified 1,327 and 1,226 cysteine-containing peptides with a false discovery rate of less than 1% for both proteins and peptides. They isolated 1,098 cysteine-containing peptides derived from 820 distinct proteins for further analysis. Of these, 195 peptides met the <span>researchers’</span> criteria for identification as redox-sensitive. These included 164 cysteines from 150 proteins that became more oxidized in the treated sample and 31 cysteines from 29 proteins that became more reduced in the treated sample. The researchers note that their previous study using gel-based analysis identified only 20 redox-sensitive proteins,<sup>7</sup> indicating that the OxiTRAQ approach is more sensitive and capable of identifying low-abundant transcription factors.</p> <p>The research team observed that redox-sensitive proteins were less likely to be extracellular and that <span>31% were located in the chloroplasts and 26% were located in the mitochondria</span> (as compared to 28% and 16%, respectively, for total proteins). They also noted that the identified redox-sensitive proteins operate in various systems, including transcription regulation, protein synthesis and metabolism.</p> <p><span><span>Liu et al. assert that the OxiTRAQ approach may be used to isolate oxidatively modified proteins in plants that have undergone physiological stress, such as infection. However, stress treatment that alters the abundance of any given redox-sensitive protein may complicate results. The researchers indicate that tissue samples undergoing extended stress treatment may require parallel proteomic evaluation to accurately determine protein abundance. Overall, they offer OxiTRAQ as a sensitive, cost-effective proteomic solution for the quantitation of redox-sensitive proteins across various plant species.</span></span></p> <p><strong>References</strong></p> <p><span>1. Apel, K., and Hirt, H. (2004) “<a href="http://www.ncbi.nlm.nih.gov/pubmed/15377225" target="_blank">Reactive oxygen species: Metabolism, oxidative stress, and signal </a></span><a href="http://www.ncbi.nlm.nih.gov/pubmed/15377225" target="_blank">transduction</a>,” Annual Review of Plant Biology, 55 (pp. 373–99).</p> <p>2. Møller, I.M., et al. (2007) “<a href="http://www.ncbi.nlm.nih.gov/pubmed/17288534" target="_blank">Oxidative modifications to cellular components in plants</a>,” Annual Review of Plant Biology, 58 (pp. 459–81).</p> <p>3. Foyer, C.H., and Noctor, G. (2005) “<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167537/" target="_blank">Redox homeostasis and antioxidant signaling: A metabolic interface between stress perception and physiological responses</a>,” Plant Cell, 17 (pp. 1866–75).</p> <p>4. Gygi, S.P., et al. (2000) “<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC16874/" target="_blank">Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology</a>,” Proceedings of the National Academy of Sciences, 97 (pp. 9390–5).</p> <p>5. Ong, S.E., and Pandey, A. (2001) “<a href="http://www.ncbi.nlm.nih.gov/pubmed/11911086" target="_blank">An evaluation of the use of two-dimensional gel electrophoresis in proteomics</a>,” Biomolecular Engineering, 18 (pp. 195–205).</p> <p>6. Liu, P., et al. (2013) “<a href="http://onlinelibrary.wiley.com/doi/10.1002/pmic.201300307/abstract" target="_blank">Identification of redox-sensitive cysteines in the Arabidopsis proteome using OxiTRAQ, a quantitative redox proteomics method</a>,” Proteomics, doi: 10.1002/pmic.201300307.</p> <p><span><span><span><span>7. Wang, H., et al. (2012) “<a href="http://www.ncbi.nlm.nih.gov/pubmed/22050424" target="_blank">Proteomic analysis of early-responsive redox-sensitive proteins in Arabidopsis</a>,” Journal of Proteome Research, 11 (pp. 412–24).</span></span></span></span></p> <p><i>Post Author: Melissa J. Mayer. Melissa is a freelance writer who specializes in science journalism. She possesses passion for and experience in the fields of proteomics, cellular/molecular biology, microbiology, biochemistry, and immunology. Melissa is also bilingual (Spanish) and holds a teaching certificate with a biology endorsement.</i></p> <aside class="mashsb-container mashsb-main "><div class="mashsb_above_buttons">Share this article </div><div class="mashsb-box"><div class="mashsb-count mash-large" style="float:left"><div class="counts mashsbcount">10</div><span class="mashsb-sharetext">SHARES</span></div><div class="mashsb-buttons"><a class="mashicon-facebook mash-large mashsb-noshadow" href="https://www.facebook.com/sharer.php?u=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fproteomics%2Foxitraq-a-novel-approach-to-identify-redox-sensitive-proteins%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Facebook</span></a><a class="mashicon-linkedin mash-large mashsb-noshadow" href="https://www.linkedin.com/shareArticle?trk=OxiTRAQ%3A+A+Novel+Approach+to+Identify+Redox-Sensitive+Proteins&url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fproteomics%2Foxitraq-a-novel-approach-to-identify-redox-sensitive-proteins%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Linkedin</span></a><div class="onoffswitch2 mash-large mashsb-noshadow" style="display:none"></div></div> </div> <div style="clear:both"></div><div class="mashsb-toggle-container"></div></aside> </div></article> <div id="respond" class="comment-respond"> <h3 id="reply-title" class="comment-reply-title">Leave a Reply <small><a rel="nofollow" id="cancel-comment-reply-link" href="/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/#respond" style="display:none;">Cancel reply</a></small></h3><form action="https://www.thermofisher.com/blog/proteomics/wp-comments-post.php" method="post" id="commentform" class="comment-form" novalidate><p class="comment-notes"><span id="email-notes">Your email address will not be published.</span> <span class="required-field-message">Required fields are marked <span class="required">*</span></span></p><p class="comment-form-comment"><label for="comment">Comment <span class="required">*</span></label> <textarea id="comment" name="comment" cols="45" rows="8" maxlength="65525" required></textarea></p><p class="comment-form-author"><label for="author">Name <span class="required">*</span></label> <input id="author" name="author" type="text" value="" size="30" maxlength="245" autocomplete="name" required /></p> <p class="comment-form-email"><label for="email">Email <span class="required">*</span></label> <input id="email" name="email" type="email" value="" size="30" maxlength="100" aria-describedby="email-notes" autocomplete="email" required /></p> <p class="comment-form-url"><label for="url">Website</label> <input id="url" name="url" type="url" value="" size="30" maxlength="200" autocomplete="url" /></p> <p class="form-submit"><input name="submit" type="submit" id="submit" class="submit" value="Post Comment" /> <input type='hidden' name='comment_post_ID' value='2476' id='comment_post_ID' /> <input type='hidden' name='comment_parent' id='comment_parent' value='0' /> </p><p style="display: none;"><input type="hidden" id="akismet_comment_nonce" name="akismet_comment_nonce" value="cd70d6bff7" /></p><p style="display: none !important;" class="akismet-fields-container" data-prefix="ak_"><label>Δ<textarea name="ak_hp_textarea" cols="45" rows="8" maxlength="100"></textarea></label><input type="hidden" id="ak_js_2" name="ak_js" value="225"/><script>document.getElementById( "ak_js_2" ).setAttribute( "value", ( new Date() ).getTime() );</script></p></form> </div> <div class="adjacent-entry-pagination pagination"><div class="pagination-previous alignleft"><i class="fa fa-icon fa-chevron-left"></i> <a href="https://www.thermofisher.com/blog/proteomics/combined-omics-approach-identifies-functional-receptor-for-hepatitis-b-virus/" rel="prev">Combined 鈥搊mics Approach Identifies Functional Receptor for Hepatitis B Virus</a></div><div class="pagination-next alignright"><a href="https://www.thermofisher.com/blog/proteomics/examining-chromatin-binding-proteins-from-colon-adenomas-and-carcinomas/" rel="next">Examining Chromatin-binding Proteins from Colon Adenomas and Carcinomas</a> <i class="fa fa-icon fa-chevron-right"></i></div></div></main></div></div><footer class="site-footer"><div class="wrap"><div class="creds"><p class="footer-links"><a href="https://corporate.thermofisher.com/en/footer/privacy-statement.html" target="_blank" rel="noopener noreferrer">Privacy Statement</a><a href="https://corporate.thermofisher.com/en/footer/terms-and-conditions.html" target="_blank" rel="noopener noreferrer">Terms & Conditions</a><a href="/blog/locations/">Locations</a><a href="/blog/sitemap/">Sitemap</a></p><p>© 2024 <a href="https://www.thermofisher.com">Thermo Fisher Scientific</a>. All Rights Reserved.</p></div><span class="social-links"><p>Talk to us</p><a href="https://www.facebook.com/thermofisher" class="facebook" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-facebook"></i></a><a href="https://www.linkedin.com/company/thermo-fisher-scientific" class="linkedin" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-linkedin"></i></a><a href="https://twitter.com/thermofisher" class="twitter" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-twitter"></i></a><a href="https://www.youtube.com/c/thermofisher" class="youtube" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-youtube"></i></a></span></div></footer></div><div style="display: none;"><div id="boxzilla-box-9233-content"><p><h4>Get news and research reviews on the topic of your choice, right in your inbox.</h4> </p> <p><a id="subscribe" class="button" href="#">Subscribe Now</a></p> <div id="subscribe-form"> <div class='gf_browser_ie gf_browser_ie7 gform_wrapper gform_legacy_markup_wrapper gform-theme--no-framework' data-form-theme='legacy' data-form-index='1' id='gform_wrapper_1' ><form method='post' enctype='multipart/form-data' id='gform_1' action='/blog/proteomics/oxitraq-a-novel-approach-to-identify-redox-sensitive-proteins/' formid='1'> <div class='gform-body gform_body'><ul id='gform_fields_1' class='gform_fields top_label form_sublabel_above description_below validation_below'><li id="field_1_4" class="gfield gfield--type-text first-name field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_4" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_4'>First Name</label><div class='ginput_container ginput_container_text'><input name='input_4' id='input_1_4' type='text' value='' class='large' placeholder='First Name' aria-invalid="false" /> </div></li><li id="field_1_5" class="gfield gfield--type-text last-name field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_5" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_5'>Last Name</label><div class='ginput_container ginput_container_text'><input name='input_5' id='input_1_5' type='text' value='' class='large' placeholder='Last Name' aria-invalid="false" /> </div></li><li id="field_1_2" class="gfield gfield--type-email email gfield_contains_required field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_2" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_2'>Email<span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></label><div class='ginput_container ginput_container_email'> <input name='input_2' id='input_1_2' type='email' value='' class='large' placeholder='Email' aria-required="true" aria-invalid="false" /> </div></li><li id="field_1_6" class="gfield gfield--type-text company field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_6" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_6'>Company</label><div class='ginput_container ginput_container_text'><input name='input_6' id='input_1_6' type='text' value='' class='large' placeholder='Company' aria-invalid="false" /> </div></li><li id="field_1_7" class="gfield gfield--type-select country gfield_contains_required field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_7" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_7'>Select your country<span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></label><div class='ginput_container ginput_container_select'><select name='input_7' id='input_1_7' class='large gfield_select' aria-required="true" aria-invalid="false" ><option value='' selected='selected' class='gf_placeholder'>Select your country</option><option value='United States' >United States</option><option value='Canada' >Canada</option><option value='Afghanistan' >Afghanistan</option><option value='Albania' >Albania</option><option value='Algeria' >Algeria</option><option value='American Samoa' >American Samoa</option><option value='Andorra' >Andorra</option><option value='Angola' >Angola</option><option value='Anguilla' >Anguilla</option><option value='Antarctica' >Antarctica</option><option value='Antigua and Barbuda' >Antigua and Barbuda</option><option value='Argentina' >Argentina</option><option value='Armenia' >Armenia</option><option value='Aruba' >Aruba</option><option value='Australia' >Australia</option><option value='Austria' >Austria</option><option value='Azerbaijan' >Azerbaijan</option><option value='Bahamas' >Bahamas</option><option value='Bahrain' >Bahrain</option><option value='Bangladesh' >Bangladesh</option><option value='Barbados' >Barbados</option><option value='Belarus' >Belarus</option><option value='Belgium' >Belgium</option><option value='Belize' >Belize</option><option value='Benin' >Benin</option><option value='Bermuda' >Bermuda</option><option value='Bhutan' >Bhutan</option><option value='Bolivia' >Bolivia</option><option value='Bosnia and Herzegovina' >Bosnia and Herzegovina</option><option value='Botswana' >Botswana</option><option value='Bouvet Island' >Bouvet Island</option><option value='Brazil' >Brazil</option><option value='British Indian Ocean Territory' >British Indian Ocean Territory</option><option value='Brunei Darussalam' >Brunei Darussalam</option><option value='Bulgaria' >Bulgaria</option><option value='Burkina Faso' >Burkina Faso</option><option value='Burundi' >Burundi</option><option value='Cambodia' >Cambodia</option><option value='Cameroon' >Cameroon</option><option value='Cape Verde' >Cape Verde</option><option value='Cayman Islands' >Cayman Islands</option><option value='Central African Republic' >Central African Republic</option><option value='Chad' >Chad</option><option value='Chile' >Chile</option><option value='China' >China</option><option value='Christmas Island' >Christmas Island</option><option value='Cocos (Keeling) Islands' >Cocos (Keeling) Islands</option><option value='Colombia' >Colombia</option><option value='Comoros' >Comoros</option><option value='Congo' >Congo</option><option value='Congo, The Democratic Republic of' >Congo, The Democratic Republic of</option><option value='Cook Islands' >Cook Islands</option><option value='Costa Rica' >Costa Rica</option><option value='Cote D'Ivoire' >Cote D'Ivoire</option><option value='Croatia' >Croatia</option><option value='Cuba' >Cuba</option><option value='Cyprus' >Cyprus</option><option value='Czech Republic' >Czech Republic</option><option value='Denmark' >Denmark</option><option value='Djibouti' >Djibouti</option><option value='Dominica' >Dominica</option><option value='Dominican Republic' >Dominican Republic</option><option value='East Timor' >East Timor</option><option value='Ecuador' >Ecuador</option><option value='Egypt' >Egypt</option><option value='El Salvador' >El Salvador</option><option value='Equatorial Guinea' >Equatorial Guinea</option><option value='Eritrea' >Eritrea</option><option value='Estonia' >Estonia</option><option value='Ethiopia' >Ethiopia</option><option value='Falkland Islands (Malvinas)' >Falkland Islands (Malvinas)</option><option value='Faroe Islands' >Faroe Islands</option><option value='Fiji' >Fiji</option><option value='Finland' >Finland</option><option value='Fmr Yugoslav Rep of Macedonia' >Fmr Yugoslav Rep of Macedonia</option><option value='France' >France</option><option value='French Guiana' >French Guiana</option><option value='French Polynesia' >French Polynesia</option><option value='French Southern Territories' >French Southern Territories</option><option value='Gabon' >Gabon</option><option value='Gambia' >Gambia</option><option value='Georgia' >Georgia</option><option value='Germany' >Germany</option><option value='Ghana' >Ghana</option><option value='Gibraltar' >Gibraltar</option><option value='Greece' >Greece</option><option value='Greenland' >Greenland</option><option value='Grenada' >Grenada</option><option value='Guadeloupe' >Guadeloupe</option><option value='Guam' >Guam</option><option value='Guatemala' >Guatemala</option><option value='Guinea' >Guinea</option><option value='Guinea-Bissau' >Guinea-Bissau</option><option value='Guyana' >Guyana</option><option value='Haiti' >Haiti</option><option value='Heard and McDonald Islands' >Heard and McDonald Islands</option><option value='Holy See (Vatican City State)' >Holy See (Vatican City State)</option><option value='Honduras' >Honduras</option><option value='Hong Kong' >Hong Kong</option><option value='Hungary' >Hungary</option><option value='Iceland' >Iceland</option><option value='India' >India</option><option value='Indonesia' >Indonesia</option><option value='Iran (Islamic Republic Of)' >Iran (Islamic Republic Of)</option><option value='Iraq' >Iraq</option><option value='Ireland' >Ireland</option><option value='Israel' >Israel</option><option value='Italy' >Italy</option><option value='Jamaica' >Jamaica</option><option value='Japan' >Japan</option><option value='Jordan' >Jordan</option><option value='Kazakstan' >Kazakstan</option><option value='Kenya' >Kenya</option><option value='Kiribati' >Kiribati</option><option value='Korea, Democratic People's Rep' >Korea, Democratic People's Rep</option><option value='Korea, Republic of' >Korea, Republic of</option><option value='Kuwait' >Kuwait</option><option value='Kyrgyzstan' >Kyrgyzstan</option><option value='Lao People's Democratic Rep' >Lao People's Democratic Rep</option><option value='Latvia' >Latvia</option><option value='Lebanon' >Lebanon</option><option value='Lesotho' >Lesotho</option><option value='Liberia' >Liberia</option><option value='Libyan Arab Jamahiriya' >Libyan Arab Jamahiriya</option><option value='Liechtenstein' >Liechtenstein</option><option value='Lithuania' >Lithuania</option><option value='Luxembourg' >Luxembourg</option><option value='Macau' >Macau</option><option value='Madagascar' >Madagascar</option><option value='Malawi' >Malawi</option><option value='Malaysia' >Malaysia</option><option value='Maldives' >Maldives</option><option value='Mali' >Mali</option><option value='Malta' >Malta</option><option value='Marshall Islands' >Marshall Islands</option><option value='Martinique' >Martinique</option><option value='Mauritania' >Mauritania</option><option value='Mauritius' >Mauritius</option><option value='Mayotte' >Mayotte</option><option value='Mexico' >Mexico</option><option value='Micronesia, Federated States' >Micronesia, Federated States</option><option value='Moldova, Republic of' >Moldova, Republic of</option><option value='Monaco' >Monaco</option><option value='Mongolia' >Mongolia</option><option value='Montserrat' >Montserrat</option><option value='Morocco' >Morocco</option><option value='Mozambique' >Mozambique</option><option value='Myanmar' >Myanmar</option><option value='Namibia' >Namibia</option><option value='Nauru' >Nauru</option><option value='Nepal' >Nepal</option><option value='Netherlands' >Netherlands</option><option value='Netherlands Antilles' >Netherlands Antilles</option><option value='New Caledonia' >New Caledonia</option><option value='New Zealand' >New Zealand</option><option value='Nicaragua' >Nicaragua</option><option value='Niger' >Niger</option><option value='Nigeria' >Nigeria</option><option value='Niue' >Niue</option><option value='Norfolk Island' >Norfolk Island</option><option value='Northern Mariana Islands' >Northern Mariana Islands</option><option value='Norway' >Norway</option><option value='Oman' >Oman</option><option value='Pakistan' >Pakistan</option><option value='Palau' >Palau</option><option value='Panama' >Panama</option><option value='Papua New Guinea' >Papua New Guinea</option><option value='Paraguay' >Paraguay</option><option value='Peru' >Peru</option><option value='Philippines' >Philippines</option><option value='Pitcairn' >Pitcairn</option><option value='Poland' >Poland</option><option value='Portugal' >Portugal</option><option value='Puerto Rico' >Puerto Rico</option><option value='Qatar' >Qatar</option><option value='Reunion' >Reunion</option><option value='Romania' >Romania</option><option value='Russian Federation' >Russian Federation</option><option value='Rwanda' >Rwanda</option><option value='Saint Helena' >Saint Helena</option><option value='Saint Kitts and Nevis' >Saint Kitts and Nevis</option><option value='Saint Lucia' >Saint Lucia</option><option value='Saint Pierre and Miquelon' >Saint Pierre and Miquelon</option><option value='Samoa' >Samoa</option><option value='San Marino' >San Marino</option><option value='Sao Tome and Principe' >Sao Tome and Principe</option><option value='Saudi Arabia' >Saudi Arabia</option><option value='Senegal' >Senegal</option><option value='Seychelles' >Seychelles</option><option value='Sierra Leone' >Sierra Leone</option><option value='Singapore' >Singapore</option><option value='Slovakia' >Slovakia</option><option value='Slovenia' >Slovenia</option><option value='Solomon Islands' >Solomon Islands</option><option value='Somalia' >Somalia</option><option value='South Africa' >South Africa</option><option value='Spain' >Spain</option><option value='Sri Lanka' >Sri Lanka</option><option value='Sth Georgia & Sth Sandwich Is' >Sth Georgia & Sth Sandwich Is</option><option value='St Vincent and the Grenadines' >St Vincent and the Grenadines</option><option value='Sudan' >Sudan</option><option value='Suriname' >Suriname</option><option value='Svalbard and Jan Mayen' >Svalbard and Jan Mayen</option><option value='Swaziland' >Swaziland</option><option value='Sweden' >Sweden</option><option value='Switzerland' >Switzerland</option><option value='Syrian Arab Republic' >Syrian Arab Republic</option><option value='Taiwan, Province of China' >Taiwan, Province of China</option><option value='Tajikistan' >Tajikistan</option><option value='Tanzania, United Republic of' >Tanzania, United Republic of</option><option value='Thailand' >Thailand</option><option value='Togo' >Togo</option><option value='Tokelau' >Tokelau</option><option value='Tonga' >Tonga</option><option value='Trinidad and Tobago' >Trinidad and Tobago</option><option value='Tunisia' >Tunisia</option><option value='Turkey' >Turkey</option><option value='Turkmenistan' >Turkmenistan</option><option value='Turks and Caicos Islands' >Turks and Caicos Islands</option><option value='Tuvalu' >Tuvalu</option><option value='Uganda' >Uganda</option><option value='Ukraine' >Ukraine</option><option value='United Arab Emirates' >United Arab Emirates</option><option value='United Kingdom' >United Kingdom</option><option value='Uruguay' >Uruguay</option><option value='US Minor Outlying Islands' >US Minor Outlying Islands</option><option value='Uzbekistan' >Uzbekistan</option><option value='Vanuatu' >Vanuatu</option><option value='Venezuela' >Venezuela</option><option value='Vietnam' >Vietnam</option><option value='Virgin Islands (British)' >Virgin Islands (British)</option><option value='Virgin Islands (U.S.)' >Virgin Islands (U.S.)</option><option value='Wallis and Futuna Islands' >Wallis and Futuna Islands</option><option value='Western Sahara' >Western Sahara</option><option value='Yemen' >Yemen</option><option value='Yugoslavia' >Yugoslavia</option><option value='Zambia' >Zambia</option><option value='Zimbabwe' >Zimbabwe</option></select></div></li><li id="field_1_8" class="gfield gfield--type-select sub-category gfield_contains_required field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_8" ><label class='gfield_label gform-field-label screen-reader-text' for='input_1_8'>Pick a category<span class="gfield_required"><span class="gfield_required gfield_required_asterisk">*</span></span></label><div class='ginput_container ginput_container_select'><select name='input_8' id='input_1_8' class='large gfield_select' aria-required="true" aria-invalid="false" ><option value='' selected='selected' class='gf_placeholder'>Pick a category</option><option value='Accelerating Microscopy' >Accelerating Microscopy</option><option value='Accelerating Proteomics' selected='selected'>Accelerating Proteomics</option><option value='Advancing Materials' >Advancing Materials</option><option value='Advancing Mining' >Advancing Mining</option><option value='Analyzing Metals' >Analyzing Metals</option><option value='Behind the Bench' >Behind the Bench</option><option value='Examining Food' >Examining Food</option><option value='Identifying Threats' >Identifying Threats</option><option value='Life in the Lab' >Life in the Lab</option><option value='The Connected Lab' >The Connected Lab</option></select></div></li><li id="field_1_9" class="gfield gfield--type-checkbox gfield--type-choice field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_9" ><label class='gfield_label gform-field-label screen-reader-text gfield_label_before_complex' ></label><div class='ginput_container ginput_container_checkbox'><ul class='gfield_checkbox' id='input_1_9'><li class='gchoice gchoice_1_9_1'> <input class='gfield-choice-input' name='input_9.1' type='checkbox' value='1' id='choice_1_9_1' /> <label for='choice_1_9_1' id='label_1_9_1' class='gform-field-label gform-field-label--type-inline'>Please confirm you would like to receive marketing and promotional messages about Thermo Fisher Scientific products and services.</label> </li></ul></div></li><li id="field_1_10" class="gfield gfield--type-hidden gform_hidden field_sublabel_above gfield--no-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_10" ><div class='ginput_container ginput_container_text'><input name='input_10' id='input_1_10' type='hidden' class='gform_hidden' aria-invalid="false" value='Proteomics' /></div></li><li id="field_1_11" class="gfield gfield--type-honeypot gform_validation_container field_sublabel_above gfield--has-description field_description_below field_validation_below gfield_visibility_visible" data-js-reload="field_1_11" ><label class='gfield_label gform-field-label' for='input_1_11'>Phone</label><div class='ginput_container'><input name='input_11' id='input_1_11' type='text' value='' autocomplete='new-password'/></div><div class='gfield_description' id='gfield_description_1_11'>This field is for validation purposes and should be left unchanged.</div></li></ul></div> <div class='gform_footer top_label'> <input type='submit' id='gform_submit_button_1' class='gform_button button' value='Subscribe' onclick='if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} ' onkeypress='if( event.keyCode == 13 ){ if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} jQuery("#gform_1").trigger("submit",[true]); }' /> <input type='hidden' class='gform_hidden' name='is_submit_1' value='1' /> <input type='hidden' class='gform_hidden' name='gform_submit' value='1' /> <input type='hidden' class='gform_hidden' name='gform_unique_id' value='' /> <input type='hidden' class='gform_hidden' name='state_1' value='WyJbXSIsImFiYmI5YzY2YWJjNDlmYzYwZjUzMTBiZjQ0NGIwZGNmIl0=' /> <input type='hidden' class='gform_hidden' name='gform_target_page_number_1' id='gform_target_page_number_1' value='0' /> <input type='hidden' class='gform_hidden' name='gform_source_page_number_1' id='gform_source_page_number_1' value='1' /> <input type='hidden' name='gform_field_values' value='' /> </div> <p style="display: none !important;" class="akismet-fields-container" data-prefix="ak_"><label>Δ<textarea name="ak_hp_textarea" cols="45" rows="8" maxlength="100"></textarea></label><input type="hidden" id="ak_js_3" name="ak_js" value="82"/><script>document.getElementById( "ak_js_3" ).setAttribute( "value", ( new Date() ).getTime() );</script></p></form> </div><script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() {gformInitSpinner( 1, 'https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery('#gform_ajax_frame_1').on('load',function(){var contents = jQuery(this).contents().find('*').html();var is_postback = contents.indexOf('GF_AJAX_POSTBACK') >= 0;if(!is_postback){return;}var form_content = jQuery(this).contents().find('#gform_wrapper_1');var is_confirmation = jQuery(this).contents().find('#gform_confirmation_wrapper_1').length > 0;var is_redirect = contents.indexOf('gformRedirect(){') >= 0;var is_form = form_content.length > 0 && ! is_redirect && ! is_confirmation;var mt = parseInt(jQuery('html').css('margin-top'), 10) + parseInt(jQuery('body').css('margin-top'), 10) + 100;if(is_form){jQuery('#gform_wrapper_1').html(form_content.html());if(form_content.hasClass('gform_validation_error')){jQuery('#gform_wrapper_1').addClass('gform_validation_error');} else {jQuery('#gform_wrapper_1').removeClass('gform_validation_error');}setTimeout( function() { /* delay the scroll by 50 milliseconds to fix a bug in chrome */ }, 50 );if(window['gformInitDatepicker']) {gformInitDatepicker();}if(window['gformInitPriceFields']) {gformInitPriceFields();}var current_page = jQuery('#gform_source_page_number_1').val();gformInitSpinner( 1, 'https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery(document).trigger('gform_page_loaded', [1, current_page]);window['gf_submitting_1'] = false;}else if(!is_redirect){var confirmation_content = jQuery(this).contents().find('.GF_AJAX_POSTBACK').html();if(!confirmation_content){confirmation_content = contents;}jQuery('#gform_wrapper_1').replaceWith(confirmation_content);jQuery(document).trigger('gform_confirmation_loaded', [1]);window['gf_submitting_1'] = false;wp.a11y.speak(jQuery('#gform_confirmation_message_1').text());}else{jQuery('#gform_1').append(contents);if(window['gformRedirect']) {gformRedirect();}}jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "current_page", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, current_page] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: current_page } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } );} ); /* ]]> */ </script> </div> </div></div><script>if(window.location.search.indexOf('post_id')!=-1){ window.close();}jQuery(document).ready(function ($) { $('p').mashSelectionSharer();});</script>  <script type="text/html" id="wpls--tmpl"> <li class="search-result"> <a href="<%= post.link %>"> <div class="wpls--item-title-wrap"> <h4 class="wpls--item-title"><%= post.title %></h4> </div> </a> </li> </script> <script type="text/javascript"> /* MonsterInsights Scroll Tracking */ if ( typeof(jQuery) !== 'undefined' ) { jQuery( document ).ready(function(){ function monsterinsights_scroll_tracking_load() { if ( ( typeof(__gaTracker) !== 'undefined' && __gaTracker && __gaTracker.hasOwnProperty( "loaded" ) && __gaTracker.loaded == true ) || ( typeof(__gtagTracker) !== 'undefined' && __gtagTracker ) ) { (function(factory) { factory(jQuery); }(function($) { /* Scroll Depth */ "use strict"; var defaults = { percentage: true }; var $window = $(window), cache = [], scrollEventBound = false, lastPixelDepth = 0; /* * Plugin */ $.scrollDepth = function(options) { var startTime = +new Date(); options = $.extend({}, defaults, options); /* * Functions */ function sendEvent(action, label, scrollDistance, timing) { if ( 'undefined' === typeof MonsterInsightsObject || 'undefined' === typeof MonsterInsightsObject.sendEvent ) { return; } var paramName = action.toLowerCase(); var fieldsArray = { send_to: 'G-RHDLB4CBWB', non_interaction: true }; fieldsArray[paramName] = label; if (arguments.length > 3) { fieldsArray.scroll_timing = timing MonsterInsightsObject.sendEvent('event', 'scroll_depth', fieldsArray); } else { MonsterInsightsObject.sendEvent('event', 'scroll_depth', fieldsArray); } } function calculateMarks(docHeight) { return { '25%' : parseInt(docHeight * 0.25, 10), '50%' : parseInt(docHeight * 0.50, 10), '75%' : parseInt(docHeight * 0.75, 10), /* Cushion to trigger 100% event in iOS */ '100%': docHeight - 5 }; } function checkMarks(marks, scrollDistance, timing) { /* Check each active mark */ $.each(marks, function(key, val) { if ( $.inArray(key, cache) === -1 && scrollDistance >= val ) { sendEvent('Percentage', key, scrollDistance, timing); cache.push(key); } }); } function rounded(scrollDistance) { /* Returns String */ return (Math.floor(scrollDistance/250) * 250).toString(); } function init() { bindScrollDepth(); } /* * Public Methods */ /* Reset Scroll Depth with the originally initialized options */ $.scrollDepth.reset = function() { cache = []; lastPixelDepth = 0; $window.off('scroll.scrollDepth'); bindScrollDepth(); }; /* Add DOM elements to be tracked */ $.scrollDepth.addElements = function(elems) { if (typeof elems == "undefined" || !$.isArray(elems)) { return; } $.merge(options.elements, elems); /* If scroll event has been unbound from window, rebind */ if (!scrollEventBound) { bindScrollDepth(); } }; /* Remove DOM elements currently tracked */ $.scrollDepth.removeElements = function(elems) { if (typeof elems == "undefined" || !$.isArray(elems)) { return; } $.each(elems, function(index, elem) { var inElementsArray = $.inArray(elem, options.elements); var inCacheArray = $.inArray(elem, cache); if (inElementsArray != -1) { options.elements.splice(inElementsArray, 1); } if (inCacheArray != -1) { cache.splice(inCacheArray, 1); } }); }; /* * Throttle function borrowed from: * Underscore.js 1.5.2 * http://underscorejs.org * (c) 2009-2013 Jeremy Ashkenas, DocumentCloud and Investigative Reporters & Editors * Underscore may be freely distributed under the MIT license. */ function throttle(func, wait) { var context, args, result; var timeout = null; var previous = 0; var later = function() { previous = new Date; timeout = null; result = func.apply(context, args); }; return function() { var now = new Date; if (!previous) previous = now; var remaining = wait - (now - previous); context = this; args = arguments; if (remaining <= 0) { clearTimeout(timeout); timeout = null; previous = now; result = func.apply(context, args); } else if (!timeout) { timeout = setTimeout(later, remaining); } return result; }; } /* * Scroll Event */ function bindScrollDepth() { scrollEventBound = true; $window.on('scroll.scrollDepth', throttle(function() { /* * We calculate document and window height on each scroll event to * account for dynamic DOM changes. */ var docHeight = $(document).height(), winHeight = window.innerHeight ? window.innerHeight : $window.height(), scrollDistance = $window.scrollTop() + winHeight, /* Recalculate percentage marks */ marks = calculateMarks(docHeight), /* Timing */ timing = +new Date - startTime; checkMarks(marks, scrollDistance, timing); }, 500)); } init(); }; /* UMD export */ return $.scrollDepth; })); jQuery.scrollDepth(); } else { setTimeout(monsterinsights_scroll_tracking_load, 200); } } monsterinsights_scroll_tracking_load(); }); } /* End MonsterInsights Scroll Tracking */ </script><script type="text/javascript" id="wpls-script-js-extra"> /* <![CDATA[ */ var wp_search_vars = {"helperText":"one more character","api":"https:\/\/admin.acceleratingscience.com\/wp-json\/as\/v1\/all-posts\/2"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/wp-search-live/public/assets/js/wp-live-search.js?ver=0.9" id="wpls-script-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/comment-reply.min.js?ver=6.6.1" id="comment-reply-js" async="async" data-wp-strategy="async"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/dom-ready.min.js?ver=f77871ff7694fffea381" id="wp-dom-ready-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/hooks.min.js?ver=2810c76e705dd1a53b18" id="wp-hooks-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/i18n.min.js?ver=5e580eb46a90c2b997e6" id="wp-i18n-js"></script> <script type="text/javascript" id="wp-i18n-js-after"> /* <![CDATA[ */ wp.i18n.setLocaleData( { 'text direction\u0004ltr': [ 'ltr' ] } ); /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/a11y.min.js?ver=d90eebea464f6c09bfd5" id="wp-a11y-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/js/placeholders.jquery.min.js?ver=2.8.17" id="gform_placeholder-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/assets/js/dist/vendor-theme.min.js?ver=54e7080aa7a02c83aa61fae430b9d869" id="gform_gravityforms_theme_vendors-js"></script> <script type="text/javascript" id="gform_gravityforms_theme-js-extra"> /* <![CDATA[ */ var gform_theme_config = {"common":{"form":{"honeypot":{"version_hash":"4a792b070322e711c2521ee9802c04ea"}}},"hmr_dev":"","public_path":"https:\/\/admin.acceleratingscience.com\/proteomics\/wp-content\/plugins\/gravityforms\/assets\/js\/dist\/"}; /* ]]> */ </script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/gravityforms/assets/js/dist/scripts-theme.min.js?ver=bab19fd84843dabc070e73326d787910" id="gform_gravityforms_theme-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/ui/core.min.js?ver=1.13.3" id="jquery-ui-core-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/ui/tabs.min.js?ver=1.13.3" id="jquery-ui-tabs-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/themes/accelerating-science-blogs/js/scripts.js?ver=1.2.9.3" id="scripts-js"></script> <script type="text/javascript" id="boxzilla-js-extra"> /* <![CDATA[ */ var boxzilla_options = {"testMode":"","boxes":[{"id":9233,"icon":"×","content":"","css":{"position":"bottom-right"},"trigger":{"method":"percentage","value":35},"animation":"fade","cookie":{"triggered":0,"dismissed":0},"rehide":false,"position":"bottom-right","screenWidthCondition":null,"closable":true,"post":{"id":9233,"title":"Single Call to Action","slug":"single-call-to-action"}}]}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/boxzilla/assets/js/script.js?ver=3.3.1" id="boxzilla-js"></script> <script defer type="text/javascript" src="https://www.thermofisher.com/blog/proteomics/wp-content/plugins/akismet/_inc/akismet-frontend.js?ver=1722950842" id="akismet-frontend-js"></script> <script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() { jQuery(document).on('gform_post_render', function(event, formId, currentPage){if(formId == 1) {gformInitChosenFields('#input_1_7,#input_1_8','No results matched');if(typeof Placeholders != 'undefined'){ Placeholders.enable(); }} } );jQuery(document).on('gform_post_conditional_logic', function(event, formId, fields, isInit){gformInitChosenFields('#input_1_7,#input_1_8','No results matched');} ) } ); /* ]]> */ </script> <script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() {jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "1", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, 1] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: 1 } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } ); /* ]]> */ </script> <script type="text/javascript" src="/-JVF2Mt77XrPue0oWQ_DN-r_/XEYukNG8mrG8EimE/LFhk/MBsdTQN/rVQM"></script></body></html>