GDP-Azido-Fucose (ES101) by R&D Systems, Part of Bio-Techne

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<div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" title="Our capillary electrophoresis instruments (icIEF and CE-SDS) provide reproducible quantitative analysis for thorough therapeutic protein characterization." class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single-Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> <a href="/applications/crispr" class="dropdown-item text-wrap" 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data-drupal-link-system-path="node/1452">Organoid and 3-D Culture</a> <a href="/research-areas/stem-cells" class="dropdown-item text-wrap" data-drupal-link-system-path="node/161">Stem Cells</a> <a href="/research-areas/targeted-protein-degradation" class="dropdown-item text-wrap" data-drupal-link-system-path="node/376">Targeted Protein Degradation</a> <a href="/research-areas" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6741">View All Research Areas</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-therapeutic-manufacturing" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Therapeutic Manufacturing <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-therapeutic-manufacturing" aria-labelledby="navbarDropdown-therapeutic-manufacturing"> <div class="row"> <div class="col 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Proteins</a> <a href="/gmp-products/gmp-small-molecules" class="dropdown-item text-wrap" data-drupal-link-system-path="node/366">GMP Small Molecules</a> <a href="/gmp-products/gmp-antibodies" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13266">GMP Antibodies</a> <a href="/gmp-products/gmp-capabilities" class="dropdown-item text-wrap" title="GMP Capabilities" data-drupal-link-system-path="node/1071">GMP Capabilities</a> <a href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2066">Immuno-Oncology</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-diagnostics" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Diagnostics <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-diagnostics" aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1281">Custom Cell & Gene Therapy Services</a> <a href="/services/custom-compound-library-service" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2126">Custom Compound Library Services</a> <a href="/services/custom-elisa-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/261">Custom ELISA Services</a> <a href="/services/gene-engineering-services-tcbuster" class="dropdown-item text-wrap" title="Gene Engineering Services: Bio-Techne" data-drupal-link-system-path="node/1136">Custom Gene Engineering Services</a> <a href="/services/custom-luminex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/271">Custom Luminex Services</a> <a href="/services/custom-protein-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1441">Custom Protein Services</a> <a href="/services/custom-simple-plex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/certified-service-provider-program" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5446">Certified Service Provider (CSP) Program</a> <a href="/resources/instrument-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5471">Contract Services</a> <a href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup Tool</a> <a href="/resources/rnascope-citations" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/rnascope-citations">RNAscope Citations</a> <a href="/resources/simple-plex-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-plex-panel-builder">Simple Plex Panel Builder</a> <a href="/resources/simple-western-kit-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-kit-builder">Simple Western Kit Builder</a> <a href="/resources/simple-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-antibody-database">Simple Western Antibody Database</a> <a href="/resources/single-cell-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/single-cell-western-antibody-database">Single-Cell Western Antibody Database</a> <a href="/resources/spectra-viewer" class="dropdown-item text-wrap" 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class="sr-only">Loading...</span></div> </div> </div> <div id="video-tile-wrapper"><article class="product-data-wrapper"> <div class="video_tile_wrapper"> <article> <div> <lite-youtube videoid="9QE6w3gImsM" style="background-image: url('https://img.youtube.com/vi/9QE6w3gImsM/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/9QE6w3gImsM?autoplay=1" class="lty-playbtn" title="Setting the standard in quality research reagents"> <span class="lyt-visually-hidden">Setting the standard in quality research reagents</span> </a> </lite-youtube> </div> </article> </div> </article> </div> <div id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> </div> <div class="tab-content rounded-bottom border-0 mb-3 shadow"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="technical-data-tab-tab" href="#technical-data-tab" data-toggle="tab" role="tab" 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jump-anchor active" href="#product-summary" id="product-summary_li" >Product Summary</a> </li> <li id="product-specifications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#product-specifications" id="product-specifications_li" >Product Specifications</a> </li> <li id="preparation--storage_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#preparation--storage" id="preparation--storage_li" >Preparation & Storage</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="product-summary" class="nav-link px-0" data-anchor-label="Product Summary"><div id="product_title_summary"><h3>Summary for GDP-Azido-Fucose</h3><article class="product-data-wrapper"> <html><head><style type="text/css">.divTextMargin {display:none} img {border: none;} .psp-header > div {float:left; width:48%;} </style> </head><body><div class="psp-header"> <div class="psp-benefits"><img alt="GDP-Azido-Fucose Structure" src="//resources.rndsystems.com/images/site/gdp-azido-fucose-structure.png" title="GDP-Azido-Fucose Structure" width="250"></div> <div> <table cellpadding="0" cellspacing="5" width="100%"> <tbody> <tr> <td> <p><strong>Formula</strong></p> </td> <td> <p>C<sub>16</sub>H<sub>24</sub>N<sub>8</sub>O<sub>15</sub>P<sub>2</sub></p> </td> </tr> <tr> <td> <p><strong>Molecular Weight</strong></p> </td> <td> <p>630.35 Da</p> </td> </tr> <tr> <td> <p><strong>Formulation</strong></p> </td> <td> <p>1 mM Provided in 20 mM Tris, 50 mM NaBorate, pH 9.0</p> </td> </tr> </tbody> </table> </div> </div> <div style="clear: both;"> </div> <p>Incorporate or detect fucose without expensive, specialized equipment!</p> <h4>Applications</h4> <ul> <li>For <em>in vitro</em> enzymatic incorporation of azido-sugars into specific, targeted glycans.</li> <li>Detecting the presence or absence of fucosylated glycans.</li> <li>Tagging bio-molecules through fucosylation.</li> <li>Assessing the level of fucosylation on target molecules.</li> </ul> <h4>Key Features and Benefits</h4> <ul> <li>Can assess for the presence of terminal fucosylation (e.g. core fucose) without expensive, specialized equipment.</li> <li>Can be introduced to proteins and lipids that have fucosylation sites via fucosyltransferases.</li> <li>Can be conjugated to desired reporter molecules via click chemistry.</li> <li>Can be detected via Western blot, ELISA, and flow cytometry, depending on the type of reporter molecule.</li> <li>Contains the smallest possible orthogonal functional group.</li> <li>Has minimal side effects on target molecules.</li> <li>User-friendly.</li> </ul> <h4>For Details:<br> <a href="https://www.sciencedirect.com/science/article/pii/S0008621515001330" target="_blank" class="a___link-external">Wu, ZL et al., (2015) Carbohydrate Res. 412:1-6.</a><br> <a href="https://academic.oup.com/glycob/article/30/12/970/5815178?login=true">Wu, ZL. et al., (2020) Glycobiology 30:970.</a></h4> <h3>Related Reagents</h3> <h4>Click Chemistry</h4> <ul> <li><a href="https://www.rndsystems.com/products/es100" target="_blank" class="a___link-external">Biotinylated Alkyne</a></li> <li><a href="https://www.rndsystems.com/products/es102" target="_blank" class="a___link-external">CMP-Azido-Sialic Acid</a></li> <li><a href="https://www.rndsystems.com/products/es103" target="_blank" class="a___link-external">UDP-Azido-GalNAc</a></li> <li><a href="https://www.rndsystems.com/products/es104" target="_blank" class="a___link-external">UDP-Azido-GlcNAc</a></li> </ul> <p> </p> <h4>Enzymes and Detection Reagents for GDP-Azido-Fucose, ES101</h4> <table cellpadding="3" cellspacing="0" class="border" width="100%"> <tbody> <tr> <td class="blue_high" width="40%"> <p><strong>Fucosyltransferases</strong></p> </td> <td class="blue_high" width="30%"> <p><strong>Fucosylation<br> Specificity/Linkage</strong></p> </td> <td class="blue_high" width="30%"> <p><strong>Notes</strong></p> </td> </tr> <tr> <td><a href="/search?keywords=protein%20o-fucosyltransferase%201/pofut1">Protein O-Fucosyltransferase 1/POFUT1</a></td> <td>Adds Fucose to Ser/Thr</td> <td>Important for Notch signaling</td> </tr> <tr class="lt_gray"> <td><a href="/search?keywords=fucosyltransferase%202/fut2">Fucosyltransferase 2/FUT2</a></td> <td>Adds (terminal) Fucose to Galactose</td> <td>Generates H antigen</td> </tr> <tr> <td><a href="/search?keywords=fucosyltransferase%203/fut3">Fucosyltransferase 3/FUT3</a></td> <td>Adds (terminal) Fucose to GlcNAc</td> <td>Generates Le<sup>a</sup>, Le<sup>b</sup> antigens</td> </tr> <tr class="lt_gray"> <td><a href="/search?keywords=fucosyltransferase%205/fut5">Fucosyltransferase 5/FUT5</a></td> <td>Adds (terminal) Fucose to Galactose</td> <td>Generates sLe<sup>x</sup> antigen</td> </tr> <tr> <td> <p>Fucosyltransferase 6/FUT6</p> </td> <td> </td> <td>Generates sLe<sup>x</sup> antigen</td> </tr> <tr class="lt_gray"> <td><a href="/search?keywords=fucosyltransferase%207/fut7">Fucosyltransferase 7/FUT7</a></td> <td>Adds (terminal) Fucose to GlcNAc</td> <td>Generates sLe<sup>x</sup> antigen</td> </tr> <tr> <td><a href="/search?keywords=fucosyltransferase%208/fut8">Fucosyltransferase 8/FUT8</a></td> <td>Adds (non-terminal) Fucose to GlcNAc</td> <td> </td> </tr> <tr class="lt_gray"> <td> <p>Fucosyltransferase 9/FUT9</p> </td> <td> </td> <td>Generates sLe<sup>x</sup> antigen</td> </tr> <tr> <td><a href="/search?keywords=fucosyltransferase%2011/fut11">Fucosyltransferase 11/FUT11</a></td> <td>Adds (non-terminal) Fucose to GlcNAc</td> <td> </td> </tr> </tbody> </table> <p> </p> <ul> <li><a href="/search?keywords=alpha-l-fucosidase">alpha-L-Fucosidase</a></li> <li><a href="/search?keywords=tissue%20alpha-l-fucosidase/fuca1">Tissue alpha-L-Fucosidase/FUCA1</a></li> <li><a href="/p/elisa-kits/streptavidin-hrp_dy998">Streptavidin-HRP</a></li> </ul> <div class="clear"> </div> <p> </p> <h3><strong>Schematic</strong></h3> <table cellspacing="0"> <tbody> <tr> <td valign="top"> <p><a class="a___link-external" data-gallery="cm-data" data-title="GDP-Azido-Fucose" data-toggle="lightbox" href="//resources.rndsystems.com/images/site/gdp-azido-fucose2-schematicv2.png?v=2" target="_blank"><img alt="GDP-Azido-Fucose" onclick="event.preventDefault();" src="//resources.rndsystems.com/images/site/gdp-azido-fucose2-schematicv2.png?v=2" width="232"><br> </a></p> </td> <td style="border: currentColor; border-image: none;" valign="top"> <p><strong>The terminal fucose</strong> can be removed using a Fucosidase. Azido-fucose can then be added to open sites with specific fucosyltransferases and detected using <a href="https://www.rndsystems.com/products/es100" target="_blank" class="a___link-external">Biotinylated Alkyne</a> in a click chemistry reaction.</p> </td> </tr> </tbody> </table> <h3><strong>Sample Data</strong></h3> <table cellspacing="0"> <tbody> <tr> <td valign="top"> <p><a class="a___link-external" data-gallery="cm-data" data-title="Detecting Open Fucosylation Sites on the Acceptor Substrate Recombinant Drosophila Notch EGF20" data-toggle="lightbox" href="//resources.rndsystems.com/images/site/gdp-azido-fucose-fig-a.png?v=2" target="_blank"><font color="#333333"><img alt="Detecting Open Fucosylation Sites on the Acceptor Substrate Recombinant Drosophila Notch EGF20" onclick="event.preventDefault();" src="//resources.rndsystems.com/images/site/gdp-azido-fucose-fig-a.png?v=2" width="232"><br> </font></a></p> </td> <td style="border: currentColor; border-image: none;" valign="top"> <p>Detecting Open Fucosylation Sites on the Acceptor Substrate Recombinant Drosophila Notch EGF20. <a href="/p/proteins-enzymes/recombinant-human-protein-o-fucosyltransferase-1-pofut1-cf_7409-gt">rhPOFUT1</a> at 0.5 µg of was incubated at 37 °C for one hour with increasing amounts (0.5, 1, 2, 4 µg) of <a href="/p/proteins-enzymes/recombinant-drosophila-notch-egf20-protein-cf_8117-dn">rdNotch EGF20</a> repeat. The left side is a labeling reaction including GDP-Azido-Fucose. The right side serves as a negative control labeling reaction without GDP-Azido-Fucose.</p> <p>The results clearly show GDP-Azido-Fucose incorporation into the Notch EGF20 substrate on open sites for POFUT1-mediated fucosylation.</p> <p>The buffer used contained 25 mM HEPES pH 7.5, 150 mM NaCl, and 10 mM MnCl<sub>2</sub> for a total volume of 40 µl. The reactions were then conjugated for one hour with 1 nmol of <a href="https://www.rndsystems.com/products/es100" target="_blank" class="a___link-external">Biotinylated Alkyne</a> in the prescence of 100 µM CuCl<sub>2</sub> and 2 mM Ascorbic Acid for a final volume of 50 µl. All reactions were separated on 12% SDS-PAGE and then detected with <a href="/p/elisa-kits/streptavidin-hrp_dy998">Streptavidin-HRP</a>.</p> </td> </tr> </tbody> </table></body></html> </article> </div> </div> <div id="product-specifications" class="nav-link px-0" data-anchor-label="Product Specifications"><table class="tablesaw tablesaw-stack table-bordered table" id="product_specification_wrapper"><h3>Product Specifications for GDP-Azido-Fucose</h3><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Species</td> <td class="product-data-value p-3 px-4 text-break"> Multi-Species </td> </tr> </table> </div> <div id="preparation--storage" class="nav-link px-0" data-anchor-label="Preparation & Storage"><div id="cc_prep_storage"><h3>Preparation & Storage</h3><table class="table-bordered table" id="prep_storage_wrapper"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Shipping Conditions</td> <td class="product-data-value p-3 px-4 text-break"> The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store the unopened product at < -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Good for 12 months from date of receipt. </td> </tr> </table> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4 show" id="procedure-tab" data-tab-label="Procedure" role="tabpanel" aria-labelledby="procedure-tab-tab"><div id="cell_culture_assay_procedure"><h3>Assay Procedure</h3><article class="product-data-wrapper"> <html><head><meta charset="utf-8"> <title></title> <style type="text/css">.psp-procedure {margin:20px 0; clear:both; border-top:1px solid #efefef;} .psp-procedure > div {float:left; width:48%; padding:5px;} .psp-step </style> </head><body><div class="grid_11"> <p>Sample Protocol for Core-6 Fucose Labeling</p> <p><strong>Protocols are guidelines. Parameters need to be optimized by end users.</strong></p> </div> <div style="clear: both;"> </div> <div class="grid_11" style="margin-left: 2px;"> <h4>Materials</h4> <ul> <li>Assay Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM MnCl<sub>2</sub>, pH 7.5.</li> <li>Protein Sample</li> <li><a href="/p/proteins-enzymes/recombinant-human-fucosyltransferase-8-fut8-protein-cf_5768-gt">Recombinant Human Fucosyltransferase 8/FUT8 (R&D Systems, Catalog # 5768-GT)</a></li> <li>GDP-Azido-Fucose (R&D Systems, Catalog # ES101)</li> <li><a href="https://www.rndsystems.com/products/es100" target="_blank" class="a___link-external">Biotinylated Alkyne (R&D Systems, Catalog # ES100)</a></li> <li>CuCl<sub>2</sub>, 1 mM in deionized water</li> <li>Ascorbic Acid, 20 mM in deionized water</li> <li>SDS-PAGE and Western blot reagents or equivalent</li> <li>TBST buffer: 25 mM Tris, 137 mM NaCl, 0.1% Tween-20, pH 7.5</li> <li><a href="/p/elisa-kits/streptavidin-hrp_dy998">Streptavidin-HRP (R&D Systems, Catalog # DY998)</a></li> </ul> </div> <div class="grid_7" style="margin-left: 2px;"> <h3>Assay Procedure</h3> <div class="psp-procedure"> <p><strong>1. Prepare</strong> a reaction mixture by combining 5 µg of Protein Sample, 1 µg of rhFUT8 in the presence of 1 nmol GDP-Azido-Fucose in the Assay Buffer with the final volume of 25 µL.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>2. Prepare</strong> negative controls according to step 1 but omit Protein Sample or rhFUT8 or GDP-Azido-Fucose.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>3. Incubate</strong> all the reactions and the controls at 37°C for one hour.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>4. To each of the samples, add</strong> 5 µL of 1 mM CuCl<sub>2</sub>, 5 µL of 20 mM Ascorbic Acid, and 5 µL of 1 mM Biotinylated Alkyne. Mix with gentle tapping.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>5. Incubate</strong> all samples at room temperature for 1 hour.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>6. Separate</strong> the reactions and controls by 12% SDS-PAGE.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>7. Blot</strong> the gel to a nitrocellulose membrane.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>8. Block</strong> the blot with 10% fat-free milk for 5 minutes.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>9. Thoroughly wash</strong> the membrane with TBST buffer by changing buffer three times for a total of 45 minutes.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>10. Incubate</strong> the blot with 25 ng/mL Streptavidin-HRP in 30 mL TBST buffer for 30 minutes.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>11. Thoroughly wash</strong> the membrane with TBST buffer by changing buffer three times for a total of 45 minutes.</p> </div> <p> </p> <div class="psp-procedure"> <p><strong>12. Detect</strong> with commercial ECL (Enhanced Chemiluminescence) reagents.</p> </div> </div> <div class="grid_11" style="margin-left: 2px;"> <h3>Final Assay Conditions Per Reaction</h3> <ul> <li>GDP-Azido-Fucose: 1 nmol</li> <li>rhFUT8: 1 µg</li> <li>Protein Sample: 5 µg</li> <li>Reaction volume: 25 µl</li> </ul> <h3>Click Chemistry Reaction Conditions Per Reaction</h3> <ul> <li>CuCl<sub>2</sub>: 5 nmol</li> <li>Ascorbic Acid: 100 nmol</li> <li>Biotinylated Alkyne: 5 nmol</li> <li>Reaction volume: 40 µl</li> </ul> </div></body></html> </article> </div> </div> <div class="tab-pane fade mx-4 py-4 show" id="citations-reviews-tab" data-tab-label="Citations / Reviews" role="tabpanel" aria-labelledby="citations-reviews-tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#reviews"> Reviews </a> </li> </div> </div> <div id="citations"> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="ES101"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> </div> <div class="mt-4" id="reviews"> <h2 class="section-title"> Customer Reviews for GDP-Azido-Fucose </h2> <div class="pp_filter_list border-bottom mb-4 pb-3"> <div class="row mb-4 pb-4"> <div class="col-md-6"> <p class="mt-2"> There are currently no reviews for this product. 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