CINXE.COM

<!DOCTYPE html> <html lang="en" dir="ltr"> <head>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script>  <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript>    <title>Search results for: four-point probe</title> <meta name="description" content="Search results for: four-point probe"> <meta name="keywords" content="four-point probe"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="four-point probe" name="q" aria-label="Search"> <button class="btn btn-light my-2 my-sm-0" type="submit"><i class="fas fa-search"></i></button> </form> </div> <div class="collapse navbar-collapse mt-1" id="navbarMenu"> <ul class="navbar-nav ml-auto align-items-center" id="mainNavMenu"> <li class="nav-item"> <a class="nav-link" href="https://waset.org/conferences" title="Conferences in 2024/2025/2026">Conferences</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/disciplines" title="Disciplines">Disciplines</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/committees" rel="nofollow">Committees</a> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownPublications" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Publications </a> <div class="dropdown-menu" aria-labelledby="navbarDropdownPublications"> <a class="dropdown-item" href="https://publications.waset.org/abstracts">Abstracts</a> <a class="dropdown-item" href="https://publications.waset.org">Periodicals</a> <a class="dropdown-item" href="https://publications.waset.org/archive">Archive</a> </div> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/page/support" title="Support">Support</a> </li> </ul> </div> </div> </nav> </div> </header> <main> <div class="container mt-4"> <div class="row"> <div class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="four-point probe"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 436</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: four-point probe</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">406</span> Measuring Oxygen Transfer Coefficients in Multiphase Bioprocesses: The Challenges and the Solution</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Peter%20G.%20Hollis">Peter G. Hollis</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20G.%20Clarke"> Kim G. Clarke</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Accurate quantification of the overall volumetric oxygen transfer coefficient (KLa) is ubiquitously measured in bioprocesses by analysing the response of dissolved oxygen (DO) to a step change in the oxygen partial pressure in the sparge gas using a DO probe. Typically, the response lag (τ) of the probe has been ignored in the calculation of KLa when τ is less than the reciprocal KLa, failing which a constant τ has invariably been assumed. These conventions have now been reassessed in the context of multiphase bioprocesses, such as a hydrocarbon-based system. Here, significant variation of τ in response to changes in process conditions has been documented. Experiments were conducted in a 5 L baffled stirred tank bioreactor (New Brunswick) in a simulated hydrocarbon-based bioprocess comprising a C14-20 alkane-aqueous dispersion with suspended non-viable Saccharomyces cerevisiae solids. DO was measured with a polarographic DO probe fitted with a Teflon membrane (Mettler Toledo). The DO concentration response to a step change in the sparge gas oxygen partial pressure was recorded, from which KLa was calculated using a first order model (without incorporation of τ) and a second order model (incorporating τ). τ was determined as the time taken to reach 63.2% of the saturation DO after the probe was transferred from a nitrogen saturated vessel to an oxygen saturated bioreactor and is represented as the inverse of the probe constant (KP). The relative effects of the process parameters on KP were quantified using a central composite design with factor levels typical of hydrocarbon bioprocesses, namely 1-10 g/L yeast, 2-20 vol% alkane and 450-1000 rpm. A response surface was fitted to the empirical data, while ANOVA was used to determine the significance of the effects with a 95% confidence interval. KP varied with changes in the system parameters with the impact of solid loading statistically significant at the 95% confidence level. Increased solid loading reduced KP consistently, an effect which was magnified at high alkane concentrations, with a minimum KP of 0.024 s-1 observed at the highest solids loading of 10 g/L. This KP was 2.8 fold lower that the maximum of 0.0661 s-1 recorded at 1 g/L solids, demonstrating a substantial increase in τ from 15.1 s to 41.6 s as a result of differing process conditions. Importantly, exclusion of KP in the calculation of KLa was shown to under-predict KLa for all process conditions, with an error up to 50% at the highest KLa values. Accurate quantification of KLa, and therefore KP, has far-reaching impact on industrial bioprocesses to ensure these systems are not transport limited during scale-up and operation. This study has shown the incorporation of τ to be essential to ensure KLa measurement accuracy in multiphase bioprocesses. Moreover, since τ has been conclusively shown to vary significantly with process conditions, it has also been shown that it is essential for τ to be determined individually for each set of process conditions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=effect%20of%20process%20conditions" title="effect of process conditions">effect of process conditions</a>, <a href="https://publications.waset.org/abstracts/search?q=measuring%20oxygen%20transfer%20coefficients" title=" measuring oxygen transfer coefficients"> measuring oxygen transfer coefficients</a>, <a href="https://publications.waset.org/abstracts/search?q=multiphase%20bioprocesses" title=" multiphase bioprocesses"> multiphase bioprocesses</a>, <a href="https://publications.waset.org/abstracts/search?q=oxygen%20probe%20response%20lag" title=" oxygen probe response lag"> oxygen probe response lag</a> </p> <a href="https://publications.waset.org/abstracts/57297/measuring-oxygen-transfer-coefficients-in-multiphase-bioprocesses-the-challenges-and-the-solution" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57297.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">266</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">405</span> Vibroacoustic Modulation with Chirp Signal</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dong%20Liu">Dong Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> By sending a high-frequency probe wave and a low-frequency pump wave to a specimen, the vibroacoustic method evaluates the defect’s severity according to the modulation index of the received signal. Many studies experimentally proved the significant sensitivity of the modulation index to the tiny contact type defect. However, it has also been found that the modulation index was highly affected by the frequency of probe or pump waves. Therefore, the chirp signal has been introduced to the VAM method since it can assess multiple frequencies in a relatively short time duration, so the robustness of the VAM method could be enhanced. Consequently, the signal processing method needs to be modified accordingly. Various studies utilized different algorithms or combinations of algorithms for processing the VAM signal method by chirp excitation. These signal process methods were compared and used for processing a VAM signal acquired from the steel samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=vibroacoustic%20modulation" title="vibroacoustic modulation">vibroacoustic modulation</a>, <a href="https://publications.waset.org/abstracts/search?q=nonlinear%20acoustic%20modulation" title=" nonlinear acoustic modulation"> nonlinear acoustic modulation</a>, <a href="https://publications.waset.org/abstracts/search?q=nonlinear%20acoustic%20NDT%26E" title=" nonlinear acoustic NDT&E"> nonlinear acoustic NDT&E</a>, <a href="https://publications.waset.org/abstracts/search?q=signal%20processing" title=" signal processing"> signal processing</a>, <a href="https://publications.waset.org/abstracts/search?q=structural%20health%20monitoring" title=" structural health monitoring"> structural health monitoring</a> </p> <a href="https://publications.waset.org/abstracts/155764/vibroacoustic-modulation-with-chirp-signal" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/155764.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">99</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">404</span> Numerical Simulation of Phase Transfer during Cryosurgery for an Irregular Tumor Using Hybrid Approach</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rama%20Bhargava">Rama Bhargava</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the current paper, numerical simulation has been performed for the two-dimensional time dependent Pennes’ heat transfer model which is solved for irregular diseased tumor cells. An elliptic cryoprobe of varying sizes is taken at the center of the computational domain in such a manner that the location of the probe is fixed throughout the computation. The phase transition occurs due to the effect of probe with infusion of different nanoparticles Au, Al₂O₃, Fe₃O₄. The cooling performance of these nanoparticles injected at very low temperature, has been studied by implementing a hybrid FEM/EFGM method in which the whole domain is decomposed into two subdomains. The results are shown in terms of temperature profile inside the computational domain. Rate of cooling is obtained for various nanoparticles and it is observed that infusion of Au nanoparticles is very much efficient in increasing the heating rate than other nanoparticles. Such numerical scheme has direct applications where the domain is irregular. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cryosurgery" title="cryosurgery">cryosurgery</a>, <a href="https://publications.waset.org/abstracts/search?q=hybrid%20EFGM%2FFEM" title=" hybrid EFGM/FEM"> hybrid EFGM/FEM</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=simulation" title=" simulation"> simulation</a> </p> <a href="https://publications.waset.org/abstracts/141329/numerical-simulation-of-phase-transfer-during-cryosurgery-for-an-irregular-tumor-using-hybrid-approach" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141329.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">243</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">403</span> Integrated Microsystem for Multiplexed Genosensor Detection of Biowarfare Agents</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Samuel%20B.%20Dulay">Samuel B. Dulay</a>, <a href="https://publications.waset.org/abstracts/search?q=Sandra%20Julich"> Sandra Julich</a>, <a href="https://publications.waset.org/abstracts/search?q=Herbert%20Tomaso"> Herbert Tomaso</a>, <a href="https://publications.waset.org/abstracts/search?q=Ciara%20K.%20O%27Sullivan"> Ciara K. O'Sullivan </a> </p> <p class="card-text"><strong>Abstract:</strong></p> An early, rapid and definite detection for the presence of biowarfare agents, pathogens, viruses and toxins is required in different situations which include civil rescue and security units, homeland security, military operations, public transportation securities such as airports, metro and railway stations due to its harmful effect on the human population. In this work, an electrochemical genosensor array that allows simultaneous detection of different biowarfare agents within an integrated microsystem that provides an easy handling of the technology which combines a microfluidics setup with a multiplexing genosensor array has been developed and optimised for the following targets: Bacillus anthracis, Brucella abortis and melitensis, Bacteriophage lambda, Francisella tularensis, Burkholderia mallei and pseudomallei, Coxiella burnetii, Yersinia pestis, and Bacillus thuringiensis. The electrode array was modified via co-immobilisation of a 1:100 (mol/mol) mixture of a thiolated probe and an oligoethyleneglycol-terminated monopodal thiol. PCR products from these relevant biowarfare agents were detected reproducibly through a sandwich assay format with the target hybridised between a surface immobilised probe into the electrode and a horseradish peroxidase-labelled secondary reporter probe, which provided an enzyme based electrochemical signal. The potential of the designed microsystem for multiplexed genosensor detection and cross-reactivity studies over potential interfering DNA sequences has demonstrated high selectivity using the developed platform producing high-throughput. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biowarfare%20agents" title="biowarfare agents">biowarfare agents</a>, <a href="https://publications.waset.org/abstracts/search?q=genosensors" title=" genosensors"> genosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=multipled%20detection" title=" multipled detection"> multipled detection</a>, <a href="https://publications.waset.org/abstracts/search?q=microsystem" title=" microsystem"> microsystem</a> </p> <a href="https://publications.waset.org/abstracts/42951/integrated-microsystem-for-multiplexed-genosensor-detection-of-biowarfare-agents" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42951.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">272</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">402</span> N400 Investigation of Semantic Priming Effect to Symbolic Pictures in Text</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Ousterhout">Thomas Ousterhout</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The purpose of this study was to investigate if incorporating meaningful pictures of gestures and facial expressions in short sentences of text could supplement the text with enough semantic information to produce and N400 effect when probe words incongruent to the picture were subsequently presented. Event-related potentials (ERPs) were recorded from a 14-channel commercial grade EEG headset while subjects performed congruent/incongruent reaction time discrimination tasks. Since pictures of meaningful gestures have been shown to be semantically processed in the brain in a similar manner as words are, it is believed that pictures will add supplementary information to text just as the inclusion of their equivalent synonymous word would. The hypothesis is that when subjects read the text/picture mixed sentences, they will process the images and words just like in face-to-face communication and therefore probe words incongruent to the image will produce an N400. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=EEG" title="EEG">EEG</a>, <a href="https://publications.waset.org/abstracts/search?q=ERP" title=" ERP"> ERP</a>, <a href="https://publications.waset.org/abstracts/search?q=N400" title=" N400"> N400</a>, <a href="https://publications.waset.org/abstracts/search?q=semantics" title=" semantics"> semantics</a>, <a href="https://publications.waset.org/abstracts/search?q=congruency" title=" congruency"> congruency</a>, <a href="https://publications.waset.org/abstracts/search?q=facilitation" title=" facilitation"> facilitation</a>, <a href="https://publications.waset.org/abstracts/search?q=Emotiv" title=" Emotiv"> Emotiv</a> </p> <a href="https://publications.waset.org/abstracts/48705/n400-investigation-of-semantic-priming-effect-to-symbolic-pictures-in-text" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/48705.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">258</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">401</span> Rapid Detection of Cocaine Using Aggregation-Induced Emission and Aptamer Combined Fluorescent Probe</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jianuo%20Sun">Jianuo Sun</a>, <a href="https://publications.waset.org/abstracts/search?q=Jinghan%20Wang"> Jinghan Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Sirui%20Zhang"> Sirui Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Chenhan%20Xu"> Chenhan Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongxia%20Hao"> Hongxia Hao</a>, <a href="https://publications.waset.org/abstracts/search?q=Hong%20Zhou"> Hong Zhou</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In recent years, the diversification and industrialization of drug-related crimes have posed significant threats to public health and safety globally. The widespread and increasingly younger demographics of drug users and the persistence of drug-impaired driving incidents underscore the urgency of this issue. Drug detection, a specialized forensic activity, is pivotal in identifying and analyzing substances involved in drug crimes. It relies on pharmacological and chemical knowledge and employs analytical chemistry and modern detection techniques. However, current drug detection methods are limited by their inability to perform semi-quantitative, real-time field analyses. They require extensive, complex laboratory-based preprocessing, expensive equipment, and specialized personnel and are hindered by long processing times. This study introduces an alternative approach using nucleic acid aptamers and Aggregation-Induced Emission (AIE) technology. Nucleic acid aptamers, selected artificially for their specific binding to target molecules and stable spatial structures, represent a new generation of biosensors following antibodies. Rapid advancements in AIE technology, particularly in tetraphenyl ethene-based luminous, offer simplicity in synthesis and versatility in modifications, making them ideal for fluorescence analysis. This work successfully synthesized, isolated, and purified an AIE molecule and constructed a probe comprising the AIE molecule, nucleic acid aptamers, and exonuclease for cocaine detection. The probe demonstrated significant relative fluorescence intensity changes and selectivity towards cocaine over other drugs. Using 4-Butoxytriethylammonium Bromide Tetraphenylethene (TPE-TTA) as the fluorescent probe, the aptamer as the recognition unit, and Exo I as an auxiliary, the system achieved rapid detection of cocaine within 5 mins in aqueous and urine, with detection limits of 1.0 and 5.0 µmol/L respectively. The probe-maintained stability and interference resistance in urine, enabling quantitative cocaine detection within a certain concentration range. This fluorescent sensor significantly reduces sample preprocessing time, offers a basis for rapid onsite cocaine detection, and promises potential for miniaturized testing setups. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20detection" title="drug detection">drug detection</a>, <a href="https://publications.waset.org/abstracts/search?q=aggregation-induced%20emission%20%28AIE%29" title=" aggregation-induced emission (AIE)"> aggregation-induced emission (AIE)</a>, <a href="https://publications.waset.org/abstracts/search?q=nucleic%20acid%20aptamer" title=" nucleic acid aptamer"> nucleic acid aptamer</a>, <a href="https://publications.waset.org/abstracts/search?q=exonuclease" title=" exonuclease"> exonuclease</a>, <a href="https://publications.waset.org/abstracts/search?q=cocaine" title=" cocaine"> cocaine</a> </p> <a href="https://publications.waset.org/abstracts/181415/rapid-detection-of-cocaine-using-aggregation-induced-emission-and-aptamer-combined-fluorescent-probe" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/181415.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">62</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">400</span> Instrumental Characterization of Cyanobacteria as Polyhydroxybutyrate Producer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eva%20Slaninova">Eva Slaninova</a>, <a href="https://publications.waset.org/abstracts/search?q=Diana%20Cernayova"> Diana Cernayova</a>, <a href="https://publications.waset.org/abstracts/search?q=Zuzana%20Sedrlova"> Zuzana Sedrlova</a>, <a href="https://publications.waset.org/abstracts/search?q=Katerina%20Mrazova"> Katerina Mrazova</a>, <a href="https://publications.waset.org/abstracts/search?q=Petr%20Sedlacek"> Petr Sedlacek</a>, <a href="https://publications.waset.org/abstracts/search?q=Jana%20Nebesarova"> Jana Nebesarova</a>, <a href="https://publications.waset.org/abstracts/search?q=Stanislav%20Obruca"> Stanislav Obruca</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cyanobacteria are gram-negative prokaryotes belonging to a group of photosynthetic bacteria. In comparison with heterotrophic microorganisms, cyanobacteria utilize atmospheric nitrogen and carbon dioxide without any additional substrates. This ability of these microorganisms could be employed in biotechnology for the production of bioplastics, concretely polyhydroxyalkanoates (PHAs) which are primarily accumulated as a storage material in cells in the form of intracellular granules. In this study, there two cyanobacterial cultures from genera Synechocystis were used, namely Synechocystic sp. PCC 6803 and Synechocystis salina CCALA 192. There were optimized and used several various approaches, including microscopic techniques such as cryo-scanning electron microscopy (Cryo-SEM) and transmission electron microscopy (TEM), and fluorescence lifetime imaging microscopy using Nile red as a fluorescent probe (FLIM). Due to these instrumental techniques, the morphology of intracellular space and surface of cells were characterized. The next group of methods which were employed was spectroscopic techniques such as UV-Vis spectroscopy measured in two modes (turbidimetry and integration sphere) and Fourier transform infrared spectroscopy (FTIR). All these diverse techniques were used for the detection and characterization of pigments (chlorophylls, carotenoids, phycocyanin, etc.) and PHAs, in our case poly (3-hydroxybutyrate) (P3HB). To verify results, gas chromatography (GC) was employed concretely for the determination of the amount of P3HB in biomass. Cyanobacteria were also characterized as polyhydroxybutyrate producers by flow cytometer, which could count cells and at the same time distinguish cells including P3HB and without due to fluorescent probe called BODIPY and live/dead fluorescent probe SYTO Blue. Based on results, P3HB content in cyanobacteria cells was determined, as also the overall fitness of the cells. Acknowledgment: Funding: This study was partly funded by the projectGA19-29651L of the Czech Science Foundation (GACR) and partly funded by the Austrian Science Fund (FWF), project I 4082-B25. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cyanobacteria" title="cyanobacteria">cyanobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescent%20probe" title=" fluorescent probe"> fluorescent probe</a>, <a href="https://publications.waset.org/abstracts/search?q=microscopic%20techniques" title=" microscopic techniques"> microscopic techniques</a>, <a href="https://publications.waset.org/abstracts/search?q=poly%283hydroxybutyrate%29" title=" poly(3hydroxybutyrate)"> poly(3hydroxybutyrate)</a>, <a href="https://publications.waset.org/abstracts/search?q=spectroscopy" title=" spectroscopy"> spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=chromatography" title=" chromatography"> chromatography</a> </p> <a href="https://publications.waset.org/abstracts/139809/instrumental-characterization-of-cyanobacteria-as-polyhydroxybutyrate-producer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139809.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">229</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">399</span> Atom Probe Study of Early Stage of Precipitation on Binary Al-Li, Al-Cu Alloys and Ternary Al-Li-Cu Alloys</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muna%20Khushaim">Muna Khushaim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aluminum-based alloys play a key role in modern engineering, especially in the aerospace industry. Introduction of solute atoms such as Li and Cu is the main approach to improve the strength in age-hardenable Al alloys via the precipitation hardening phenomenon. Knowledge of the decomposition process of the microstructure during the precipitation reaction is particularly important for future technical developments. The objective of this study is to investigate the nano-scale chemical composition in the Al-Cu, Al-Li and Al-Li-Cu during the early stage of the precipitation sequence and to describe whether this compositional difference correlates with variations in the observed precipitation kinetics. Comparing the random binomial frequency distribution and the experimental frequency distribution of concentrations in atom probe tomography data was used to investigate the early stage of decomposition in the different binary and ternary alloys which were experienced different heat treatments. The results show that an Al-1.7 at.% Cu alloy requires a long ageing time of approximately 8 h at 160 °C to allow the diffusion of Cu atoms into Al matrix. For the Al-8.2 at.% Li alloy, a combination of both the natural ageing condition (48 h at room temperature) and a short artificial ageing condition (5 min at 160 °C) induces increasing on the number density of the Li clusters and hence increase number of precipitated δ' particles. Applying this combination of natural ageing and short artificial ageing conditions onto the ternary Al-4 at.% Li-1.7 at.% Cu alloy induces the formation of a Cu-rich phase. Increasing the Li content in the ternary alloy up to 8 at.% and increasing the ageing time to 30 min resulted in the precipitation processes ending with δ' particles. Thus, the results contribute to the understanding of Al-alloy design. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aluminum%20alloy" title="aluminum alloy">aluminum alloy</a>, <a href="https://publications.waset.org/abstracts/search?q=atom%20probe%20tomography" title=" atom probe tomography"> atom probe tomography</a>, <a href="https://publications.waset.org/abstracts/search?q=early%20stage" title=" early stage"> early stage</a>, <a href="https://publications.waset.org/abstracts/search?q=decomposition" title=" decomposition"> decomposition</a> </p> <a href="https://publications.waset.org/abstracts/43640/atom-probe-study-of-early-stage-of-precipitation-on-binary-al-li-al-cu-alloys-and-ternary-al-li-cu-alloys" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43640.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">343</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">398</span> Portable Palpation Probe for Diabetic Foot Ulceration Monitoring</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bummo%20Ahn">Bummo Ahn</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Palpation is widely used to measure soft tissue firmness or stiffness in the living condition in order to apply detection, diagnosis, and treatment of tumors, scar tissue, abnormal muscle tone, or muscle spasticity. Since these methods are subjective and depend on the proficiency level, it is concluded that there are other diagnoses depending on the condition of the experts and the results are not objective. The mechanical property obtained by using the elasticity of the tissue is important to calculate a predictive variable for monitoring abnormal tissues. If the mechanical load such as reaction force on the foot increases in the same region under the same conditions, the mechanical property of the tissue is changed. Therefore, objective diagnosis is possible not only for experts but also for patients using this quantitative information. Furthermore, the portable system also allows non-experts to easily diagnose at home, not in hospitals or institutions. In this paper, we introduce a portable palpation system that can be used to measure the mechanical properties of human tissue, which can be applied to monitor diabetic foot ulceration patients with measuring the mechanical property change of foot tissue. The system was designed to be smaller and portable in comparison with the conventional palpation systems. It is consists of the probe, the force sensor, linear actuator, micro control unit, the display module, battery, and housing. Using this system, we performed validation experiments by applying different palpations (3 and 5 mm) to soft tissue (silicone rubber) and measured reaction forces. In addition, we estimated the elastic moduli of the soft tissue against different palpations and compare the estimated elastic moduli that show similar value even if the palpation depths are different. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=palpation%20probe" title="palpation probe">palpation probe</a>, <a href="https://publications.waset.org/abstracts/search?q=portable" title=" portable"> portable</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetic%20foot%20ulceration" title=" diabetic foot ulceration"> diabetic foot ulceration</a>, <a href="https://publications.waset.org/abstracts/search?q=monitoring" title=" monitoring"> monitoring</a>, <a href="https://publications.waset.org/abstracts/search?q=mechanical%20property" title=" mechanical property"> mechanical property</a> </p> <a href="https://publications.waset.org/abstracts/91632/portable-palpation-probe-for-diabetic-foot-ulceration-monitoring" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91632.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">120</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">397</span> Comparison and Validation of a dsDNA biomimetic Quality Control Reference for NGS based BRCA CNV analysis versus MLPA</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Delimitsou">A. Delimitsou</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Gouedard"> C. Gouedard</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Konstanta"> E. Konstanta</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Koletis"> A. Koletis</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Patera"> S. Patera</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Manou"> E. Manou</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Spaho"> K. Spaho</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Murray"> S. Murray</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: There remains a lack of International Standard Control Reference materials for Next Generation Sequencing-based approaches or device calibration. We have designed and validated dsDNA biomimetic reference materials for targeted such approaches incorporating proprietary motifs (patent pending) for device/test calibration. They enable internal single-sample calibration, alleviating sample comparisons to pooled historical population-based data assembly or statistical modelling approaches. We have validated such an approach for BRCA Copy Number Variation analytics using iQRS™-CNVSUITE versus Mixed Ligation-dependent Probe Amplification. Methods: Standard BRCA Copy Number Variation analysis was compared between mixed ligation-dependent probe amplification and next generation sequencing using a cohort of 198 breast/ovarian cancer patients. Next generation sequencing based copy number variation analysis of samples spiked with iQRS™ dsDNA biomimetics were analysed using proprietary CNVSUITE software. Mixed ligation-dependent probe amplification analyses were performed on an ABI-3130 Sequencer and analysed with Coffalyser software. Results: Concordance of BRCA – copy number variation events for mixed ligation-dependent probe amplification and CNVSUITE indicated an overall sensitivity of 99.88% and specificity of 100% for iQRS™-CNVSUITE. The negative predictive value of iQRS-CNVSUITE™ for BRCA was 100%, allowing for accurate exclusion of any event. The positive predictive value was 99.88%, with no discrepancy between mixed ligation-dependent probe amplification and iQRS™-CNVSUITE. For device calibration purposes, precision was 100%, spiking of patient DNA demonstrated linearity to 1% (±2.5%) and range from 100 copies. Traditional training was supplemented by predefining the calibrator to sample cut-off (lock-down) for amplicon gain or loss based upon a relative ratio threshold, following training of iQRS™-CNVSUITE using spiked iQRS™ calibrator and control mocks. BRCA copy number variation analysis using iQRS™-CNVSUITE™ was successfully validated and ISO15189 accredited and now enters CE-IVD performance evaluation. Conclusions: The inclusion of a reference control competitor (iQRS™ dsDNA mimetic) to next generation sequencing-based sequencing offers a more robust sample-independent approach for the assessment of copy number variation events compared to mixed ligation-dependent probe amplification. The approach simplifies data analyses, improves independent sample data analyses, and allows for direct comparison to an internal reference control for sample-specific quantification. Our iQRS™ biomimetic reference materials allow for single sample copy number variation analytics and further decentralisation of diagnostics to single patient sample assessment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=validation" title="validation">validation</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=oncology" title=" oncology"> oncology</a>, <a href="https://publications.waset.org/abstracts/search?q=copy%20number%20variation" title=" copy number variation"> copy number variation</a>, <a href="https://publications.waset.org/abstracts/search?q=reference%20material" title=" reference material"> reference material</a>, <a href="https://publications.waset.org/abstracts/search?q=calibration" title=" calibration"> calibration</a> </p> <a href="https://publications.waset.org/abstracts/171085/comparison-and-validation-of-a-dsdna-biomimetic-quality-control-reference-for-ngs-based-brca-cnv-analysis-versus-mlpa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171085.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">66</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">396</span> Quantitative Evaluation of Efficiency of Surface Plasmon Excitation with Grating-Assisted Metallic Nanoantenna</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Almaz%20R.%20Gazizov">Almaz R. Gazizov</a>, <a href="https://publications.waset.org/abstracts/search?q=Sergey%20S.%20Kharintsev"> Sergey S. Kharintsev</a>, <a href="https://publications.waset.org/abstracts/search?q=Myakzyum%20Kh.%20Salakhov"> Myakzyum Kh. Salakhov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work deals with background signal suppression in tip-enhanced near-field optical microscopy (TENOM). The background appears because an optical signal is detected not only from the subwavelength area beneath the tip but also from a wider diffraction-limited area of laser’s waist that might contain another substance. The background can be reduced by using a taper probe with a grating on its lateral surface where an external illumination causes surface plasmon excitation. It requires the grating with parameters perfectly matched with a given incident light for effective light coupling. This work is devoted to an analysis of the light-grating coupling and a quest of grating parameters to enhance a near-field light beneath the tip apex. The aim of this work is to find the figure of merit of plasmon excitation depending on grating period and location of grating in respect to the apex. In our consideration the metallic grating on the lateral surface of the tapered plasmonic probe is illuminated by a plane wave, the electric field is perpendicular to the sample surface. Theoretical model of efficiency of plasmon excitation and propagation toward the apex is tested by fdtd-based numerical simulation. An electric field of the incident light is enhanced on the grating by every single slit due to lightning rod effect. Hence, grating causes amplitude and phase modulation of the incident field in various ways depending on geometry and material of grating. The phase-modulating grating on the probe is a sort of metasurface that provides manipulation by spatial frequencies of the incident field. The spatial frequency-dependent electric field is found from the angular spectrum decomposition. If one of the components satisfies the phase-matching condition then one can readily calculate the figure of merit of plasmon excitation, defined as a ratio of the intensities of the surface mode and the incident light. During propagation towards the apex, surface wave undergoes losses in probe material, radiation losses, and mode compression. There is an optimal location of the grating in respect to the apex. One finds the value by matching quadratic law of mode compression and the exponential law of light extinction. Finally, performed theoretical analysis and numerical simulations of plasmon excitation demonstrate that various surface waves can be effectively excited by using the overtones of a period of the grating or by phase modulation of the incident field. The gratings with such periods are easy to fabricate. Tapered probe with the grating effectively enhances and localizes the incident field at the sample. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=angular%20spectrum%20decomposition" title="angular spectrum decomposition">angular spectrum decomposition</a>, <a href="https://publications.waset.org/abstracts/search?q=efficiency" title=" efficiency"> efficiency</a>, <a href="https://publications.waset.org/abstracts/search?q=grating" title=" grating"> grating</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20plasmon" title=" surface plasmon"> surface plasmon</a>, <a href="https://publications.waset.org/abstracts/search?q=taper%20nanoantenna" title=" taper nanoantenna"> taper nanoantenna</a> </p> <a href="https://publications.waset.org/abstracts/63233/quantitative-evaluation-of-efficiency-of-surface-plasmon-excitation-with-grating-assisted-metallic-nanoantenna" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63233.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">283</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">395</span> Human Vibrotactile Discrimination Thresholds for Simultaneous and Sequential Stimuli</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Joanna%20Maj">Joanna Maj</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Body machine interfaces (BMIs) afford users a non-invasive way coordinate movement. Vibrotactile stimulation has been incorporated into BMIs to allow feedback in real-time and guide movement control to benefit patients with cognitive deficits, such as stroke survivors. To advance research in this area, we examined vibrational discrimination thresholds at four body locations to determine suitable application sites for future multi-channel BMIs using vibration cues to guide movement planning and control. Twelve healthy adults had a pair of small vibrators (tactors) affixed to the skin at each location: forearm, shoulders, torso, and knee. A "standard" stimulus (186 Hz; 750 ms) and "probe" stimuli (11 levels ranging from 100 Hz to 235 Hz; 750 ms) were delivered. Probe and test stimulus pairs could occur sequentially or simultaneously (timing). Participants verbally indicated which stimulus felt more intense. Stimulus order was counterbalanced across tactors and body locations. Probabilities that probe stimuli felt more intense than the standard stimulus were computed and fit with a cumulative Gaussian function; the discrimination threshold was defined as one standard deviation of the underlying distribution. Threshold magnitudes depended on stimulus timing and location. Discrimination thresholds were better for stimuli applied sequentially vs. simultaneously at the torso as well as the knee. Thresholds were small (better) and relatively insensitive to timing differences for vibrations applied at the shoulder. BMI applications requiring multiple channels of simultaneous vibrotactile stimulation should therefore consider the shoulder as a deployment site for a vibrotactile BMI interface. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electromyography" title="electromyography">electromyography</a>, <a href="https://publications.waset.org/abstracts/search?q=electromyogram" title=" electromyogram"> electromyogram</a>, <a href="https://publications.waset.org/abstracts/search?q=neuromuscular%20disorders" title=" neuromuscular disorders"> neuromuscular disorders</a>, <a href="https://publications.waset.org/abstracts/search?q=biomedical%20instrumentation" title=" biomedical instrumentation"> biomedical instrumentation</a>, <a href="https://publications.waset.org/abstracts/search?q=controls%20engineering" title=" controls engineering"> controls engineering</a> </p> <a href="https://publications.waset.org/abstracts/156951/human-vibrotactile-discrimination-thresholds-for-simultaneous-and-sequential-stimuli" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156951.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">64</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">394</span> Development of Folding Based Aptasensor for Ochratoxin a Using Different Pulse Voltammetry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rupesh%20K.%20Mishra">Rupesh K. Mishra</a>, <a href="https://publications.waset.org/abstracts/search?q=Ga%C3%ABlle%20Catanante"> Gaëlle Catanante</a>, <a href="https://publications.waset.org/abstracts/search?q=Akhtar%20Hayat"> Akhtar Hayat</a>, <a href="https://publications.waset.org/abstracts/search?q=Jean-Louis%20Marty"> Jean-Louis Marty</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ochratoxins (OTA) are secondary metabolites present in a wide variety of food stuff. They are dangerous by-products mainly produced by several species of storage fungi including the Aspergillus and Penicillium genera. OTA is known to have nephrotoxic, immunotoxic, teratogenic and carcinogenic effects. Thus, needs a special attention for a highly sensitive and selective detection system that can quantify these organic toxins in various matrices such as cocoa beans. This work presents a folding based aptasensors by employing an aptamer conjugated redox probe (methylene blue) specifically designed for OTA. The aptamers were covalently attached to the screen printed carbon electrodes using diazonium grafting. Upon sensing the OTA, it binds with the immobilized aptamer on the electrode surface, which induces the conformational changes of the aptamer, consequently increased in the signal. This conformational change of the aptamer before and after biosensing of target OTA could produce the distinguishable electrochemical signal. The obtained limit of detection was 0.01 ng/ml for OTA samples with recovery of up to 88% in contaminated cocoa samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ochratoxin%20A" title="ochratoxin A">ochratoxin A</a>, <a href="https://publications.waset.org/abstracts/search?q=cocoa" title=" cocoa"> cocoa</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20aptamer" title=" DNA aptamer"> DNA aptamer</a>, <a href="https://publications.waset.org/abstracts/search?q=labelled%20probe" title=" labelled probe"> labelled probe</a> </p> <a href="https://publications.waset.org/abstracts/43825/development-of-folding-based-aptasensor-for-ochratoxin-a-using-different-pulse-voltammetry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43825.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">285</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">393</span> South African Breast Cancer Mutation Spectrum: Pitfalls to Copy Number Variation Detection Using Internationally Designed Multiplex Ligation-Dependent Probe Amplification and Next Generation Sequencing Panels </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jaco%20Oosthuizen">Jaco Oosthuizen</a>, <a href="https://publications.waset.org/abstracts/search?q=Nerina%20C.%20Van%20Der%20Merwe"> Nerina C. Van Der Merwe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The National Health Laboratory Services in Bloemfontien has been the diagnostic testing facility for 1830 patients for familial breast cancer since 1997. From the cohort, 540 were comprehensively screened using High-Resolution Melting Analysis or Next Generation Sequencing for the presence of point mutations and/or indels. Approximately 90% of these patients stil remain undiagnosed as they are BRCA1/2 negative. Multiplex ligation-dependent probe amplification was initially added to screen for copy number variation detection, but with the introduction of next generation sequencing in 2017, was substituted and is currently used as a confirmation assay. The aim was to investigate the viability of utilizing internationally designed copy number variation detection assays based on mostly European/Caucasian genomic data for use within a South African context. The multiplex ligation-dependent probe amplification technique is based on the hybridization and subsequent ligation of multiple probes to a targeted exon. The ligated probes are amplified using conventional polymerase chain reaction, followed by fragment analysis by means of capillary electrophoresis. The experimental design of the assay was performed according to the guidelines of MRC-Holland. For BRCA1 (P002-D1) and BRCA2 (P045-B3), both multiplex assays were validated, and results were confirmed using a secondary probe set for each gene. The next generation sequencing technique is based on target amplification via multiplex polymerase chain reaction, where after the amplicons are sequenced parallel on a semiconductor chip. Amplified read counts are visualized as relative copy numbers to determine the median of the absolute values of all pairwise differences. Various experimental parameters such as DNA quality, quantity, and signal intensity or read depth were verified using positive and negative patients previously tested internationally. DNA quality and quantity proved to be the critical factors during the verification of both assays. The quantity influenced the relative copy number frequency directly whereas the quality of the DNA and its salt concentration influenced denaturation consistency in both assays. Multiplex ligation-dependent probe amplification produced false positives due to ligation failure when ligation was inhibited due to a variant present within the ligation site. Next generation sequencing produced false positives due to read dropout when primer sequences did not meet optimal multiplex binding kinetics due to population variants in the primer binding site. The analytical sensitivity and specificity for the South African population have been proven. Verification resulted in repeatable reactions with regards to the detection of relative copy number differences. Both multiplex ligation-dependent probe amplification and next generation sequencing multiplex panels need to be optimized to accommodate South African polymorphisms present within the genetically diverse ethnic groups to reduce the false copy number variation positive rate and increase performance efficiency. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=familial%20breast%20cancer" title="familial breast cancer">familial breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=multiplex%20ligation-dependent%20probe%20amplification" title=" multiplex ligation-dependent probe amplification"> multiplex ligation-dependent probe amplification</a>, <a href="https://publications.waset.org/abstracts/search?q=next%20generation%20sequencing" title=" next generation sequencing"> next generation sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=South%20Africa" title=" South Africa"> South Africa</a> </p> <a href="https://publications.waset.org/abstracts/79169/south-african-breast-cancer-mutation-spectrum-pitfalls-to-copy-number-variation-detection-using-internationally-designed-multiplex-ligation-dependent-probe-amplification-and-next-generation-sequencing-panels" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/79169.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">231</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">392</span> Imaging 255nm Tungsten Thin Film Adhesion with Picosecond Ultrasonics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Abbas">A. Abbas</a>, <a href="https://publications.waset.org/abstracts/search?q=X.%20Tridon"> X. Tridon</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Michelon"> J. Michelon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the electronic or in the photovoltaic industries, components are made from wafers which are stacks of thin film layers of a few nanometers to serval micrometers thickness. Early evaluation of the bounding quality between different layers of a wafer is one of the challenges of these industries to avoid dysfunction of their final products. Traditional pump-probe experiments, which have been developed in the 70’s, give a partial solution to this problematic but with a non-negligible drawback. In fact, on one hand, these setups can generate and detect ultra-high ultrasounds frequencies which can be used to evaluate the adhesion quality of wafer layers. But, on the other hand, because of the quiet long acquisition time they need to perform one measurement, these setups remain shut in punctual measurement to evaluate global sample quality. This last point can lead to bad interpretation of the sample quality parameters, especially in the case of inhomogeneous samples. Asynchronous Optical Sampling (ASOPS) systems can perform sample characterization with picosecond acoustics up to 106 times faster than traditional pump-probe setups. This last point allows picosecond ultrasonic to unlock the acoustic imaging field at the nanometric scale to detect inhomogeneities regarding sample mechanical properties. This fact will be illustrated by presenting an image of the measured acoustical reflection coefficients obtained by mapping, with an ASOPS setup, a 255nm thin-film tungsten layer deposited on a silicone substrate. Interpretation of the coefficient reflection in terms of bounding quality adhesion will also be exposed. Origin of zones which exhibit good and bad quality bounding will be discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adhesion" title="adhesion">adhesion</a>, <a href="https://publications.waset.org/abstracts/search?q=picosecond%20ultrasonics" title=" picosecond ultrasonics"> picosecond ultrasonics</a>, <a href="https://publications.waset.org/abstracts/search?q=pump-probe" title=" pump-probe"> pump-probe</a>, <a href="https://publications.waset.org/abstracts/search?q=thin%20film" title=" thin film"> thin film</a> </p> <a href="https://publications.waset.org/abstracts/83530/imaging-255nm-tungsten-thin-film-adhesion-with-picosecond-ultrasonics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/83530.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">391</span> Probing Neuron Mechanics with a Micropipette Force Sensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Madeleine%20Anthonisen">Madeleine Anthonisen</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Hussain%20Sangji"> M. Hussain Sangji</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Monserratt%20Lopez-Ayon"> G. Monserratt Lopez-Ayon</a>, <a href="https://publications.waset.org/abstracts/search?q=Margaret%20Magdesian"> Margaret Magdesian</a>, <a href="https://publications.waset.org/abstracts/search?q=Peter%20Grutter"> Peter Grutter</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Advances in micromanipulation techniques and real-time particle tracking with nanometer resolution have enabled biological force measurements at scales relevant to neuron mechanics. An approach to precisely control and maneuver neurite-tethered polystyrene beads is presented. Analogous to an Atomic Force Microscope (AFM), this multi-purpose platform is a force sensor with imaging acquisition and manipulation capabilities. A mechanical probe composed of a micropipette with its tip fixed to a functionalized bead is used to incite the formation of a neurite in a sample of rat hippocampal neurons while simultaneously measuring the tension in said neurite as the sample is pulled away from the beaded tip. With optical imaging methods, a force resolution of 12 pN is achieved. Moreover, the advantages of this technique over alternatives such as AFM, namely ease of manipulation which ultimately allows higher throughput investigation of the mechanical properties of neurons, is demonstrated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=axonal%20growth" title="axonal growth">axonal growth</a>, <a href="https://publications.waset.org/abstracts/search?q=axonal%20guidance" title=" axonal guidance"> axonal guidance</a>, <a href="https://publications.waset.org/abstracts/search?q=force%20probe" title=" force probe"> force probe</a>, <a href="https://publications.waset.org/abstracts/search?q=pipette%20micromanipulation" title=" pipette micromanipulation"> pipette micromanipulation</a>, <a href="https://publications.waset.org/abstracts/search?q=neurite%20tension" title=" neurite tension"> neurite tension</a>, <a href="https://publications.waset.org/abstracts/search?q=neuron%20mechanics" title=" neuron mechanics"> neuron mechanics</a> </p> <a href="https://publications.waset.org/abstracts/62618/probing-neuron-mechanics-with-a-micropipette-force-sensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/62618.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">367</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">390</span> Development of an Instrument for Measurement of Thermal Conductivity and Thermal Diffusivity of Tropical Fruit Juice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20Ewetumo">T. Ewetumo</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20D.%20Adedayo"> K. D. Adedayo</a>, <a href="https://publications.waset.org/abstracts/search?q=Festus%20Ben"> Festus Ben</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Knowledge of the thermal properties of foods is of fundamental importance in the food industry to establish the design of processing equipment. However, for tropical fruit juice, there is very little information in literature, seriously hampering processing procedures. This research work describes the development of an instrument for automated thermal conductivity and thermal diffusivity measurement of tropical fruit juice using a transient thermal probe technique based on line heat principle. The system consists of two thermocouple sensors, constant current source, heater, thermocouple amplifier, microcontroller, microSD card shield and intelligent liquid crystal. A fixed distance of 6.50mm was maintained between the two probes. When heat is applied, the temperature rise at the heater probe measured with time at time interval of 4s for 240s. The measuring element conforms as closely as possible to an infinite line source of heat in an infinite fluid. Under these conditions, thermal conductivity and thermal diffusivity are simultaneously measured, with thermal conductivity determined from the slope of a plot of the temperature rise of the heating element against the logarithm of time while thermal diffusivity was determined from the time it took the sample to attain a peak temperature and the time duration over a fixed diffusivity distance. A constant current source was designed to apply a power input of 16.33W/m to the probe throughout the experiment. The thermal probe was interfaced with a digital display and data logger by using an application program written in C++. Calibration of the instrument was done by determining the thermal properties of distilled water. Error due to convection was avoided by adding 1.5% agar to the water. The instrument has been used for measurement of thermal properties of banana, orange and watermelon. Thermal conductivity values of 0.593, 0.598, 0.586 W/m^o C and thermal diffusivity values of 1.053 ×〖10〗^(-7), 1.086 ×〖10〗^(-7), and 0.959 ×〖10〗^(-7) 〖m/s〗^2 were obtained for banana, orange and water melon respectively. Measured values were stored in a microSD card. The instrument performed very well as it measured the thermal conductivity and thermal diffusivity of the tropical fruit juice samples with statistical analysis (ANOVA) showing no significant difference (p>0.05) between the literature standards and estimated averages of each sample investigated with the developed instrument. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=thermal%20conductivity" title="thermal conductivity">thermal conductivity</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20diffusivity" title=" thermal diffusivity"> thermal diffusivity</a>, <a href="https://publications.waset.org/abstracts/search?q=tropical%20fruit%20juice" title=" tropical fruit juice"> tropical fruit juice</a>, <a href="https://publications.waset.org/abstracts/search?q=diffusion%20equation" title=" diffusion equation"> diffusion equation</a> </p> <a href="https://publications.waset.org/abstracts/41922/development-of-an-instrument-for-measurement-of-thermal-conductivity-and-thermal-diffusivity-of-tropical-fruit-juice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41922.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">357</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">389</span> Temperature Dependence of Relative Permittivity: A Measurement Technique Using Split Ring Resonators</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sreedevi%20P.%20Chakyar">Sreedevi P. Chakyar</a>, <a href="https://publications.waset.org/abstracts/search?q=Jolly%20Andrews"> Jolly Andrews</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20P.%20Joseph"> V. P. Joseph </a> </p> <p class="card-text"><strong>Abstract:</strong></p> A compact method for measuring the relative permittivity of a dielectric material at different temperatures using a single circular Split Ring Resonator (SRR) metamaterial unit working as a test probe is presented in this paper. The dielectric constant of a material is dependent upon its temperature and the <em>LC</em> resonance of the SRR depends on its dielectric environment. Hence, the temperature of the dielectric material in contact with the resonator influences its resonant frequency. A single SRR placed between transmitting and receiving probes connected to a Vector Network Analyser (VNA) is used as a test probe. The dependence of temperature between 30 <sup>o</sup>C and 60 <sup>o</sup>C on resonant frequency of SRR is analysed. Relative permittivities ‘ε’ of test samples for different temperatures are extracted from a calibration graph drawn between the relative permittivity of samples of known dielectric constant and their corresponding resonant frequencies. This method is found to be an easy and efficient technique for analysing the temperature dependent permittivity of different materials. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=metamaterials" title="metamaterials">metamaterials</a>, <a href="https://publications.waset.org/abstracts/search?q=negative%20permeability" title=" negative permeability"> negative permeability</a>, <a href="https://publications.waset.org/abstracts/search?q=permittivity%20measurement%20techniques" title=" permittivity measurement techniques"> permittivity measurement techniques</a>, <a href="https://publications.waset.org/abstracts/search?q=split%20ring%20resonators" title=" split ring resonators"> split ring resonators</a>, <a href="https://publications.waset.org/abstracts/search?q=temperature%20dependent%20dielectric%20constant" title=" temperature dependent dielectric constant"> temperature dependent dielectric constant</a> </p> <a href="https://publications.waset.org/abstracts/50478/temperature-dependence-of-relative-permittivity-a-measurement-technique-using-split-ring-resonators" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/50478.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">412</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">388</span> Effect of Ultrasonic Treatment on the Suspension Stability, Zeta Potential and Contact Angle of Celestite</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kiraz%20Esmeli">Kiraz Esmeli</a>, <a href="https://publications.waset.org/abstracts/search?q=Alper%20Ozkan"> Alper Ozkan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, firstly, the effect of ultrasonic treatment on the stability of celestite suspension was investigated. In this context, the variations of the suspension stability with ultrasonic power, treatment time, immersion depth of ultrasonic probe, and treatment regime (batch and continuous) were determined. The experimental results showed that the suspension stability and zeta potential of celestite decreased with ultrasonic treatment. Also, the treatment time, immersion depth of probe, and treatment regime affected the stability of celestite suspension. Secondly, the effect of pre-treatment of the suspension with the ultrasonic process on the shear flocculation of celestite using sodium dodecyl sulfate (SDS) was studied and the variations of the flocculation, zeta potential, and contact angle of the mineral with SDS concentration were presented. It was found that the ultrasonic pre-treatment slightly improved the shear flocculation of celestite particles in accordance with the increase in the contact angles. In addition, the ultrasonic process again relatively reduced the magnitude of the negative potential of celestite particles in the presence of SDS. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=celestite" title="celestite">celestite</a>, <a href="https://publications.waset.org/abstracts/search?q=contact%20angle" title=" contact angle"> contact angle</a>, <a href="https://publications.waset.org/abstracts/search?q=suspension%20stability" title=" suspension stability"> suspension stability</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasonic%20treatment" title=" ultrasonic treatment"> ultrasonic treatment</a>, <a href="https://publications.waset.org/abstracts/search?q=zeta%20potential" title=" zeta potential"> zeta potential</a> </p> <a href="https://publications.waset.org/abstracts/89475/effect-of-ultrasonic-treatment-on-the-suspension-stability-zeta-potential-and-contact-angle-of-celestite" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/89475.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">226</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">387</span> Developing Motorized Spectroscopy System for Tissue Scanning </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tuba%20Denkceken">Tuba Denkceken</a>, <a href="https://publications.waset.org/abstracts/search?q=Ayse%20Nur%20Sar%C4%B1"> Ayse Nur Sarı</a>, <a href="https://publications.waset.org/abstracts/search?q=Volkan%20Ihsan%20Tore"> Volkan Ihsan Tore</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahmut%20Denkceken"> Mahmut Denkceken</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of the presented study was to develop a newly motorized spectroscopy system. Our system is composed of probe and motor parts. The probe part consists of bioimpedance and fiber optic components that include two platinum wires (each 25 micrometer in diameter) and two fiber cables (each 50 micrometers in diameter) respectively. Probe was examined on tissue phantom (polystyrene microspheres with different diameters). In the bioimpedance part of the probe current was transferred to the phantom and conductivity information was obtained. Adjacent two fiber cables were used in the fiber optic part of the system. Light was transferred to the phantom by fiber that was connected to the light source and backscattered light was collected with the other adjacent fiber for analysis. It is known that the nucleus expands and the nucleus-cytoplasm ratio increases during the cancer progression in the cell and this situation is one of the most important criteria for evaluating the tissue for pathologists. The sensitivity of the probe to particle (nucleus) size in phantom was tested during the study. Spectroscopic data obtained from our system on phantom was evaluated by multivariate statistical analysis. Thus the information about the particle size in the phantom was obtained. Bioimpedance and fiber optic experiments results which were obtained from polystyrene microspheres showed that the impedance value and the oscillation amplitude were increasing while the size of particle was enlarging. These results were compatible with the previous studies. In order to motorize the system within the motor part, three driver electronic circuits were designed primarily. In this part, supply capacitors were placed symmetrically near to the supply inputs which were used for balancing the oscillation. Female capacitors were connected to the control pin. Optic and mechanic switches were made. Drivers were structurally designed as they could command highly calibrated motors. It was considered important to keep the drivers’ dimension as small as we could (4.4x4.4x1.4 cm). Then three miniature step motors were connected to each other along with three drivers. Since spectroscopic techniques are quantitative methods, they yield more objective results than traditional ones. In the future part of this study, it is planning to get spectroscopic data that have optic and impedance information from the cell culture which is normal, low metastatic and high metastatic breast cancer. In case of getting high sensitivity in differentiated cells, it might be possible to scan large surface tissue areas in a short time with small steps. By means of motorize feature of the system, any region of the tissue will not be missed, in this manner we are going to be able to diagnose cancerous parts of the tissue meticulously. This work is supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK) through 3001 project (115E662). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=motorized%20spectroscopy" title="motorized spectroscopy">motorized spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=phantom" title=" phantom"> phantom</a>, <a href="https://publications.waset.org/abstracts/search?q=scanning%20system" title=" scanning system"> scanning system</a>, <a href="https://publications.waset.org/abstracts/search?q=tissue%20scanning" title=" tissue scanning"> tissue scanning</a> </p> <a href="https://publications.waset.org/abstracts/74692/developing-motorized-spectroscopy-system-for-tissue-scanning" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74692.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">191</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">386</span> Dispersion-Less All Reflective Split and Delay Unit for Ultrafast Metrology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Akansha%20Tyagi">Akansha Tyagi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mehar%20S.%20Sidhu"> Mehar S. Sidhu</a>, <a href="https://publications.waset.org/abstracts/search?q=Ankur%20Mandal"> Ankur Mandal</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanjay%20Kapoor"> Sanjay Kapoor</a>, <a href="https://publications.waset.org/abstracts/search?q=Sunil%20Dahiya"> Sunil Dahiya</a>, <a href="https://publications.waset.org/abstracts/search?q=Jan%20M.%20Rost"> Jan M. Rost</a>, <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Pfeifer"> Thomas Pfeifer</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamal%20P.%20Singh"> Kamal P. Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An all-reflective split and delay unit is designed for dispersion free measurement of broadband ultrashort pulses using a pair of reflective knife edge prism for splitting and recombining of the measuring pulse. It is based on symmetrical wavefront splitting of the measuring pulse having two separate arms to independently shape both split parts. We have validated our delay line with NIR –femtosecond pulse measurement centered at 800 nm using second harmonic-Interferometric frequency resolved optical gating (SH-IFROG). The delay line is compact, easy to align and provides attosecond stability and precision and thus make it more versatile for wide range of applications in ultrafast measurements. We envision that the present delay line will find applications in IR-IR controlling for high harmonic generation (HHG) and attosecond IR-XUV pump-probe measurements with solids and gases providing attosecond resolution and wide delay range. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=HHG" title="HHG">HHG</a>, <a href="https://publications.waset.org/abstracts/search?q=nonlinear%20optics" title=" nonlinear optics"> nonlinear optics</a>, <a href="https://publications.waset.org/abstracts/search?q=pump-probe%20spectroscopy" title=" pump-probe spectroscopy"> pump-probe spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrafast%20metrology" title=" ultrafast metrology"> ultrafast metrology</a> </p> <a href="https://publications.waset.org/abstracts/147793/dispersion-less-all-reflective-split-and-delay-unit-for-ultrafast-metrology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/147793.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">200</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">385</span> Probing Syntax Information in Word Representations with Deep Metric Learning</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bowen%20Ding">Bowen Ding</a>, <a href="https://publications.waset.org/abstracts/search?q=Yihao%20Kuang"> Yihao Kuang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In recent years, with the development of large-scale pre-trained lan-guage models, building vector representations of text through deep neural network models has become a standard practice for natural language processing tasks. From the performance on downstream tasks, we can know that the text representation constructed by these models contains linguistic information, but its encoding mode and extent are unclear. In this work, a structural probe is proposed to detect whether the vector representation produced by a deep neural network is embedded with a syntax tree. The probe is trained with the deep metric learning method, so that the distance between word vectors in the metric space it defines encodes the distance of words on the syntax tree, and the norm of word vectors encodes the depth of words on the syntax tree. The experiment results on ELMo and BERT show that the syntax tree is encoded in their parameters and the word representations they produce. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=deep%20metric%20learning" title="deep metric learning">deep metric learning</a>, <a href="https://publications.waset.org/abstracts/search?q=syntax%20tree%20probing" title=" syntax tree probing"> syntax tree probing</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20language%20processing" title=" natural language processing"> natural language processing</a>, <a href="https://publications.waset.org/abstracts/search?q=word%20representations" title=" word representations"> word representations</a> </p> <a href="https://publications.waset.org/abstracts/173855/probing-syntax-information-in-word-representations-with-deep-metric-learning" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/173855.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">68</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">384</span> Mirror-Like Effect Based on Correlations among Atoms</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Qurrat-ul-Ain%20Gulfam">Qurrat-ul-Ain Gulfam</a>, <a href="https://publications.waset.org/abstracts/search?q=Zbigniew%20Ficek"> Zbigniew Ficek</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The novel idea to use single atoms as highly reflecting mirrors has recently gained much attention. Usually, to observe the reflective nature of an atom, it is required to couple the atom to an external medium such that a directional spontaneous emission could be realized. We propose an alternative way to achieve the directional emission by considering a system of correlated atoms in free space. It is well known that mutually interacting atoms have a strong tendency to emit the radiation along particular discrete directions. That relieves one from the stingy condition of associating the atomic system to another media and facilitates the experimental implementation to a large degree. Moreover, realistic 3-dimensional collective emission can be taken into account in the dynamics. Two interesting spatial setups have been considered; one where a probe atom is confined in a linear cavity formed by two atomic mirrors and, the other where a probe atom faces a chain of correlated atoms. We observe an evidence of the mirror-like effect in a simple system of a chain of three atoms. The angular distribution of the radiation intensity observed in the far field is greatly affected by the atomic interactions. Hence, suitable directions for enhanced reflectivity can be determined. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=atom-mirror%20effect" title="atom-mirror effect">atom-mirror effect</a>, <a href="https://publications.waset.org/abstracts/search?q=correlated%20system" title=" correlated system"> correlated system</a>, <a href="https://publications.waset.org/abstracts/search?q=dipole-dipole%20interactions" title=" dipole-dipole interactions"> dipole-dipole interactions</a>, <a href="https://publications.waset.org/abstracts/search?q=intensity" title=" intensity"> intensity</a> </p> <a href="https://publications.waset.org/abstracts/23129/mirror-like-effect-based-on-correlations-among-atoms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23129.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">549</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">383</span> Triplex Detection of Pistacia vera, Arachis hypogaea and Pisum sativum in Processed Food Products Using Probe Based PCR</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Erg%C3%BCn%20%C5%9Eakalar">Ergün Şakalar</a>, <a href="https://publications.waset.org/abstracts/search?q=%C5%9Eeyma%20%C3%96z%C3%A7irak%20Erg%C3%BCn"> Şeyma Özçirak Ergün</a>, <a href="https://publications.waset.org/abstracts/search?q=Emrah%20Yalazi%CC%87"> Emrah Yalazi̇</a>, <a href="https://publications.waset.org/abstracts/search?q=Emine%20Altinkaya"> Emine Altinkaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Cengiz%20Ata%C5%9Fo%C4%9Flu"> Cengiz Ataşoğlu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In recent years, food allergies which cause serious health problems affect to public health around the world. Foodstuffs which contain allergens are either intentionally used as ingredients or are encased as contaminant in food products. The prevalence of clinical allergy to peanuts and nuts is estimated at about 0.4%-1.1% of the adult population, representing the allergy to pistachio the 7% of the cases of tree nut causing allergic reactions. In order to protect public health and enforce the legislation, methods for sensitive analysis of pistachio and peanut contents in food are required. Pea, pistachio and peanut are used together, to reduce the cost in food production such as baklava, snack foods.DNA technology-based methods in food analysis are well-established and well-roundedtools for species differentiation, allergen detection. Especially, the probe-based TaqMan real-time PCR assay can amplify target DNA with efﬁciency, speciﬁcity, and sensitivity.In this study, pistachio, peanut and pea were ﬁnely ground and three separate series of triplet mixtures containing 0.1, 1, 10, 100, 1000, 10,000 and 100,000 mg kg-1 of each sample were prepared for each series, to a ﬁnal weight of 100 g. DNA from reference samples and industrial products was successfully extracted with the GIDAGEN® Multi-Fast DNA Isolation Kit. TaqMan probes were designed for triplex determination of ITS, Ara h 3 and pea lectin genes which are speciﬁc regions for identification pistachio, peanut and pea, respectively.The real-time PCR as quantitative detected pistachio, peanut and pea in these mixtures down to the lowest investigated level of 0.1, 0.1 and 1 mg kg-1, respectively. Also, the methods reported here are capable of detecting of as little as 0.001% level of peanut DNA, 0,000001% level of pistachio DNA and 0.000001% level of pea DNA. We accomplish that the quantitative triplex real-time PCR method developed in this study canbe applied to detect pistachio, peanut and peatraces for three allergens at once in commercial food products. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=allergens" title="allergens">allergens</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA" title=" DNA"> DNA</a>, <a href="https://publications.waset.org/abstracts/search?q=real-time%20PCR" title=" real-time PCR"> real-time PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=TaqMan%20probe" title=" TaqMan probe"> TaqMan probe</a> </p> <a href="https://publications.waset.org/abstracts/53016/triplex-detection-of-pistacia-vera-arachis-hypogaea-and-pisum-sativum-in-processed-food-products-using-probe-based-pcr" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53016.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">256</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">382</span> Deformation and Crystallization in a 7075-T651 Friction Stir Weld</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=C.%20S.%20Paglia">C. S. Paglia</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The deformation and the crystallization in a 7075-T651 friction stir weld, in particular for regions directly in contact with the mechanical action of the rotating probe, have been investigated by means of optical microscopy. The investigation enabled to identify regions of the weld differently affected by the deformation caused by the welding process. The highly deformed grains in the horizontal direction close to the plate margin were indicative of shear movements along the horizontal plane, while highly deformed grains along the plate margin in the vertical direction were indicative of vertical shear movements of opposite directions, which superimposed the shear movement along the horizontal plane. The vertical shear movements were not homogeneous through the plate thickness. The microstructure indicated that after the probe passes, the grain growth may take place under static conditions. The small grains microstructure of the nugget region, formed after the main dynamic recrystallization process, develops to an equiaxed microstructure. A material transport influenced by the rotating shoulder was also observed from the trailing to the advancing side of the weld. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=AA7075-T651" title="AA7075-T651">AA7075-T651</a>, <a href="https://publications.waset.org/abstracts/search?q=friction%20stir%20welding" title=" friction stir welding"> friction stir welding</a>, <a href="https://publications.waset.org/abstracts/search?q=deformation" title=" deformation"> deformation</a>, <a href="https://publications.waset.org/abstracts/search?q=crystallization" title=" crystallization"> crystallization</a> </p> <a href="https://publications.waset.org/abstracts/124625/deformation-and-crystallization-in-a-7075-t651-friction-stir-weld" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/124625.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">120</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">381</span> Achieving Shear Wave Elastography by a Three-element Probe for Wearable Human-machine Interface</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jipeng%20Yan">Jipeng Yan</a>, <a href="https://publications.waset.org/abstracts/search?q=Xingchen%20Yang"> Xingchen Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaowei%20Zhou"> Xiaowei Zhou</a>, <a href="https://publications.waset.org/abstracts/search?q=Mengxing%20Tang"> Mengxing Tang</a>, <a href="https://publications.waset.org/abstracts/search?q=Honghai%20Liu"> Honghai Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Shear elastic modulus of skeletal muscles can be obtained by shear wave elastography (SWE) and has been linearly related to muscle force. However, SWE is currently implemented using array probes. Price and volumes of these probes and their driving equipment prevent SWE from being used in wearable human-machine interfaces (HMI). Moreover, beamforming processing for array probes reduces the real-time performance. To achieve SWE by wearable HMIs, a customized three-element probe is adopted in this work, with one element for acoustic radiation force generation and the others for shear wave tracking. In-phase quadrature demodulation and 2D autocorrelation are adopted to estimate velocities of tissues on the sound beams of the latter two elements. Shear wave speeds are calculated by phase shift between the tissue velocities. Three agar phantoms with different elasticities were made by changing the weights of agar. Values of the shear elastic modulus of the phantoms were measured as 8.98, 23.06 and 36.74 kPa at a depth of 7.5 mm respectively. This work verifies the feasibility of measuring shear elastic modulus by wearable devices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=shear%20elastic%20modulus" title="shear elastic modulus">shear elastic modulus</a>, <a href="https://publications.waset.org/abstracts/search?q=skeletal%20muscle" title=" skeletal muscle"> skeletal muscle</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasound" title=" ultrasound"> ultrasound</a>, <a href="https://publications.waset.org/abstracts/search?q=wearable%20human-machine%20interface" title=" wearable human-machine interface"> wearable human-machine interface</a> </p> <a href="https://publications.waset.org/abstracts/127469/achieving-shear-wave-elastography-by-a-three-element-probe-for-wearable-human-machine-interface" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/127469.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">161</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">380</span> Hohmann Transfer and Bi-Elliptic Hohmann Transfer in TRAPPIST-1 System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jorge%20L.%20Nisperuza">Jorge L. Nisperuza</a>, <a href="https://publications.waset.org/abstracts/search?q=Wilson%20Sandoval"> Wilson Sandoval</a>, <a href="https://publications.waset.org/abstracts/search?q=Edward.%20A.%20Gil"> Edward. A. Gil</a>, <a href="https://publications.waset.org/abstracts/search?q=Johan%20A.%20Jimenez"> Johan A. Jimenez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In orbital mechanics, an active research topic is the calculation of interplanetary trajectories efficient in terms of energy and time. In this sense, this work concerns the calculation of the orbital elements for sending interplanetary probes in the extrasolar system TRAPPIST-1. Specifically, using the mathematical expressions of the circular and elliptical trajectory parameters, expressions for the flight time and the orbital transfer rate increase between orbits, the orbital parameters and the graphs of the trajectories of Hohmann and Hohmann bi-elliptic for sending a probe from the innermost planet to all the other planets of the studied system, are obtained. The relationship between the orbital transfer rate increments and the relationship between the flight times for the two transfer types is found. The results show that, for all cases under consideration, the Hohmann transfer results to be the least energy and temporary cost, a result according to the theory associated with Hohmann and Hohmann bi-elliptic transfers. Saving in the increase of the speed reaches up to 87% was found, and it happens for the transference between the two innermost planets, whereas the time of flight increases by a factor of up to 6.6 if one makes use of the bi-elliptic transfer, this for the case of sending a probe from the innermost planet to the outermost. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bi-elliptic%20Hohmann%20transfer" title="bi-elliptic Hohmann transfer">bi-elliptic Hohmann transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=exoplanet" title=" exoplanet"> exoplanet</a>, <a href="https://publications.waset.org/abstracts/search?q=extrasolar%20system" title=" extrasolar system"> extrasolar system</a>, <a href="https://publications.waset.org/abstracts/search?q=Hohmann%20transfer" title=" Hohmann transfer"> Hohmann transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=TRAPPIST-1" title=" TRAPPIST-1"> TRAPPIST-1</a> </p> <a href="https://publications.waset.org/abstracts/98728/hohmann-transfer-and-bi-elliptic-hohmann-transfer-in-trappist-1-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/98728.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">192</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">379</span> Malate Dehydrogenase Enabled ZnO Nanowires as an Optical Tool for Malic Acid Detection in Horticultural Products </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rana%20Tabassum">Rana Tabassum</a>, <a href="https://publications.waset.org/abstracts/search?q=Ravi%20Kant"> Ravi Kant</a>, <a href="https://publications.waset.org/abstracts/search?q=Banshi%20D.%20Gupta"> Banshi D. Gupta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Malic acid is an extensively distributed organic acid in numerous horticultural products in minute amounts which significantly contributes towards taste determination by balancing sugar and acid fractions. An enhanced concentration of malic acid is utilized as an indicator of fruit maturity. In addition, malic acid is also a crucial constituent of several cosmetics and pharmaceutical products. An efficient detection and quantification protocol for malic acid is thus highly demanded. In this study, we report a novel detection scheme for malic acid by synergistically collaborating fiber optic surface plasmon resonance (FOSPR) and distinctive features of nanomaterials favorable for sensing applications. The design blueprint involves the deposition of an assembly of malate dehydrogenase enzyme entrapped in ZnO nanowires forming the sensing route over silver coated central unclad core region of an optical fiber. The formation and subsequent decomposition of the enzyme-analyte complex on exposure of the sensing layer to malic acid solutions of diverse concentration results in modification of the dielectric function of the sensing layer which is manifested in terms of shift in resonance wavelength. Optimization of experimental variables such as enzyme concentration entrapped in ZnO nanowires, dip time of probe for deposition of sensing layer and working pH range of the sensing probe have been accomplished through SPR measurements. The optimized sensing probe displays high sensitivity, broad working range and a minimum limit of detection value and has been successfully tested for malic acid determination in real samples of fruit juices. The current work presents a novel perspective towards malic acid determination as the unique and cooperative combination of FOSPR and nanomaterials provides myriad advantages such as enhanced sensitivity, specificity, compactness together with the possibility of online monitoring and remote sensing. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=surface%20plasmon%20resonance" title="surface plasmon resonance">surface plasmon resonance</a>, <a href="https://publications.waset.org/abstracts/search?q=optical%20fiber" title=" optical fiber"> optical fiber</a>, <a href="https://publications.waset.org/abstracts/search?q=sensor" title=" sensor"> sensor</a>, <a href="https://publications.waset.org/abstracts/search?q=malic%20acid" title=" malic acid"> malic acid</a> </p> <a href="https://publications.waset.org/abstracts/73444/malate-dehydrogenase-enabled-zno-nanowires-as-an-optical-tool-for-malic-acid-detection-in-horticultural-products" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/73444.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">380</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">378</span> Blood Oxygen Saturation Measurement System Using Broad-Band Light Source with LabVIEW Program</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Myoung%20Ah%20Kim">Myoung Ah Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Dong%20Ho%20Sin"> Dong Ho Sin</a>, <a href="https://publications.waset.org/abstracts/search?q=Chul%20Gyu%20Song"> Chul Gyu Song</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Blood oxygen saturation system is a well-established, noninvasive photoplethysmographic method to monitor vital signs. Conventional blood oxygen saturation measurements for the two LED light source is the ambiguity of the oxygen saturation measurement principle and the measurement results greatly influenced and heat and motion artifact. A high accuracy in order to solve these problems blood oxygen saturation measuring method has been proposed using a broadband light source that can be easily understood by the algorithm. The measurement of blood oxygen saturation based on broad-band light source has advantage of simple testing facility and easy understanding. Broadband light source based on blood oxygen saturation measuring program proposed in this paper is a combination of LabVIEW and MATLAB. Using the wavelength range of 450 nm-750 nm using a floating light absorption of oxyhemoglobin and deoxyhemoglobin to measure the blood oxygen saturation. Hand movement is to fix the probe to the motor stage in order to prevent oxygen saturation measurement that affect the sample and probe kept constant interval. Experimental results show that the proposed method noticeably increases the accuracy and saves time compared with the conventional methods. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=oxygen%20saturation" title="oxygen saturation">oxygen saturation</a>, <a href="https://publications.waset.org/abstracts/search?q=broad-band%20light%20source" title=" broad-band light source"> broad-band light source</a>, <a href="https://publications.waset.org/abstracts/search?q=CCD" title=" CCD"> CCD</a>, <a href="https://publications.waset.org/abstracts/search?q=light%20reflectance%20theory" title=" light reflectance theory"> light reflectance theory</a> </p> <a href="https://publications.waset.org/abstracts/40627/blood-oxygen-saturation-measurement-system-using-broad-band-light-source-with-labview-program" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40627.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">459</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">377</span> Analysis of the Annual Proficiency Testing Procedure for Intermediate Reference Laboratories Conducted by the National Reference Laboratory from 2013 to 2017</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reena%20K.">Reena K.</a>, <a href="https://publications.waset.org/abstracts/search?q=Mamatha%20H.%20G."> Mamatha H. G.</a>, <a href="https://publications.waset.org/abstracts/search?q=Somshekarayya"> Somshekarayya</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Kumar"> P. Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objectives: The annual proficiency testing of intermediate reference laboratories is conducted by the National Reference Laboratory (NRL) to assess the efficiency of the laboratories to correctly identify Mycobacterium tuberculosis and to determine its drug susceptibility pattern. The proficiency testing results from 2013 to 2017 were analyzed to determine laboratories that were consistent in reporting quality results and those that had difficulty in doing so. Methods: A panel of twenty cultures were sent out to each of these laboratories. The laboratories were expected to grow the cultures in their own laboratories, set up drug susceptibly testing by all the methods they were certified for and report the results within the stipulated time period. The turnaround time for reporting results, specificity, sensitivity positive and negative predictive values and efficiency of the laboratory in identifying the cultures were analyzed. Results: Most of the laboratories had reported their results within the stipulated time period. However, there was enormous delay in reporting results from few of the laboratories. This was mainly due to improper functioning of the biosafety level III laboratory. Only 40% of the laboratories had 100% efficiency in solid culture using Lowenstein Jensen medium. This was expected as a solid culture, and drug susceptibility testing is not used for diagnosing drug resistance. Rapid molecular methods such as Line probe assay and Genexpert are used to determine drug resistance. Automated liquid culture system such as the Mycobacterial growth indicator tube is used to determine prognosis of the patient while on treatment. It was observed that 90% of the laboratories had achieved 100% in the liquid culture method. Almost all laboratories had achieved 100% efficiency in the line probe assay method which is the method of choice for determining drug-resistant tuberculosis. Conclusion: Since the liquid culture and line probe assay technologies are routinely used for the detection of drug-resistant tuberculosis the laboratories exhibited higher level of efficiency as compared to solid culture and drug susceptibility testing which are rarely used. The infrastructure of the laboratory should be maintained properly so that samples can be processed safely and results could be declared on time. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=annual%20proficiency%20testing" title="annual proficiency testing">annual proficiency testing</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20susceptibility%20testing" title=" drug susceptibility testing"> drug susceptibility testing</a>, <a href="https://publications.waset.org/abstracts/search?q=intermediate%20reference%20laboratory" title=" intermediate reference laboratory"> intermediate reference laboratory</a>, <a href="https://publications.waset.org/abstracts/search?q=national%20reference%20laboratory" title=" national reference laboratory"> national reference laboratory</a> </p> <a href="https://publications.waset.org/abstracts/82990/analysis-of-the-annual-proficiency-testing-procedure-for-intermediate-reference-laboratories-conducted-by-the-national-reference-laboratory-from-2013-to-2017" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/82990.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">182</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=1" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=1">1</a></li> <li class="page-item active"><span class="page-link">2</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=6">6</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=7">7</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=8">8</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=9">9</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=10">10</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=14">14</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=15">15</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=four-point%20probe&page=3" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>