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tissue engineering</title> <meta name="description" content="Search results for: adipose tissue engineering"> <meta name="keywords" content="adipose tissue engineering"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="adipose tissue engineering"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4408</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: adipose tissue engineering</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4408</span> The Influence of Alginate Microspheres Modified with DAT on the Proliferation and Adipogenic Differentiation of ASCs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shin-Yi%20Mao">Shin-Yi Mao</a>, <a href="https://publications.waset.org/abstracts/search?q=Jiashing%20Yu"> Jiashing Yu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Decellularized adipose tissue (DAT) has received lots of attention as biological scaffolds recently. DAT that extracted from the extracellular matrix (ECM) of adipose tissues holds great promise as a xenogeneic biomaterial for tissue engineering and regenerative medicine. In our study, 2-D DATsol film was fabricated to enhance cell adhesion, proliferation, and differentiation of ASCs in vitro. DAT was also used to modify alginate for improvement of cell adhesion. Alginate microspheres modified with DAT were prepared by Nisco. These microspheres could provide a highly supportive 3-D environment for ASCs. In our works, ASCs were immobilized in alginate microspheres modified with DAT to promoted cell adhesion and adipogenic differentiation. Accordingly, we hypothesize that tissue regeneration in vivo could be promoted with the aid of modified microspheres in future. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adipose%20stem%20cells" title="adipose stem cells">adipose stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=decellularize%20adipose%20tissue" title=" decellularize adipose tissue"> decellularize adipose tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=Alginate" title=" Alginate"> Alginate</a>, <a href="https://publications.waset.org/abstracts/search?q=microcarries" title=" microcarries"> microcarries</a> </p> <a href="https://publications.waset.org/abstracts/13276/the-influence-of-alginate-microspheres-modified-with-dat-on-the-proliferation-and-adipogenic-differentiation-of-ascs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13276.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">444</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4407</span> Vascularized Adipose Tissue Engineering by Using Adipose ECM/Fibroin Hydrogel</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alisan%20Kayabolen">Alisan Kayabolen</a>, <a href="https://publications.waset.org/abstracts/search?q=Dilek%20Keskin"> Dilek Keskin</a>, <a href="https://publications.waset.org/abstracts/search?q=Ferit%20Avcu"> Ferit Avcu</a>, <a href="https://publications.waset.org/abstracts/search?q=Andac%20Aykan"> Andac Aykan</a>, <a href="https://publications.waset.org/abstracts/search?q=Fatih%20Zor"> Fatih Zor</a>, <a href="https://publications.waset.org/abstracts/search?q=Aysen%20Tezcaner"> Aysen Tezcaner</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Adipose tissue engineering is a promising field for regeneration of soft tissue defects. However, only very thin implants can be used in vivo since vascularization is still a problem for thick implants. Another problem is finding a biocompatible scaffold with good mechanical properties. In this study, the aim is to develop a thick vascularized adipose tissue that will integrate with the host, and perform its in vitro and in vivo characterizations. For this purpose, a hydrogel of decellularized adipose tissue (DAT) and fibroin was produced, and both endothelial cells and adipocytes that were differentiated from adipose derived stem cells were encapsulated in this hydrogel. Mixing DAT with fibroin allowed rapid gel formation by vortexing. It also provided to adjust mechanical strength by changing fibroin to DAT ratio. Based on compression tests, gels of DAT/fibroin ratio with similar mechanical properties to adipose tissue was selected for cell culture experiments. In vitro characterizations showed that DAT is not cytotoxic; on the contrary, it has many natural ECM components which provide biocompatibility and bioactivity. Subcutaneous implantation of hydrogels resulted with no immunogenic reaction or infection. Moreover, localized empty hydrogels gelled successfully around host vessel with required shape. Implantations of cell encapsulated hydrogels and histological analyses are under study. It is expected that endothelial cells inside the hydrogel will form a capillary network and they will bind to the host vessel passing through hydrogel. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adipose%20tissue%20engineering" title="adipose tissue engineering">adipose tissue engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=decellularization" title=" decellularization"> decellularization</a>, <a href="https://publications.waset.org/abstracts/search?q=encapsulation" title=" encapsulation"> encapsulation</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrogel" title=" hydrogel"> hydrogel</a>, <a href="https://publications.waset.org/abstracts/search?q=vascularization" title=" vascularization"> vascularization</a> </p> <a href="https://publications.waset.org/abstracts/19805/vascularized-adipose-tissue-engineering-by-using-adipose-ecmfibroin-hydrogel" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19805.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">528</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4406</span> The Effect of 8 Weeks Endurance Training and L-NAME on Apelin in Adipose Tissue, Glucose and Insulin in Elderly Male's Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asieh%20Abbassi%20Daloii">Asieh Abbassi Daloii</a>, <a href="https://publications.waset.org/abstracts/search?q=Fatemeh%20Fani"> Fatemeh Fani</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmad%20Abdi"> Ahmad Abdi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: The aim of this study was to determine the effect of 8 weeks endurance training and L-NAME on apelin in adipose tissue, glucose and insulin in elderly male’s rats. Methods: For this purpose, 24 vistar elderly rats with average 20 months old purchased from Razi Institute and transferred to Research Center were randomly divided into four groups: 1. control, 2. training, 3.training and L-NAME and 4. L-NAME. Training protocol performed for 8 weeks and 5 days a week with 75-80 VO2 max. All rats were killed 72 hours after the final training session and after 24 hours of fasting adipose tissue samples were collected and kept in -80. Also, Data was analyzed with One way ANOVA and Tucky in p < 0/05. Results: The results showed that the inhibition of nitric oxide on apelin in adipose tissue of adult male rats after eight weeks of endurance training increased significantly compared to the control group (p < 0.00). Also, the results showed no significant difference between the levels of insulin and glucose groups. Conclusion: It is likely that the increased apelin in adipose tissue in mice independent of insulin and glucose. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endurance%20training" title="endurance training">endurance training</a>, <a href="https://publications.waset.org/abstracts/search?q=L-NAME" title=" L-NAME"> L-NAME</a>, <a href="https://publications.waset.org/abstracts/search?q=apelin%20in%20adipose%20tissue" title=" apelin in adipose tissue"> apelin in adipose tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=elderly%20male%20rats" title=" elderly male rats"> elderly male rats</a> </p> <a href="https://publications.waset.org/abstracts/35354/the-effect-of-8-weeks-endurance-training-and-l-name-on-apelin-in-adipose-tissue-glucose-and-insulin-in-elderly-males-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35354.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">460</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4405</span> Stroma-Providing Activity of Adipose Derived Mesenchymal Stromal Cells in Tissue-Related O2 Microenvironment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20I.%20Bobyleva">P. I. Bobyleva</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20R.%20Andreeva"> E. R. Andreeva</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20V.%20Andrianova"> I. V. Andrianova</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20V.%20Maslova"> E. V. Maslova</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20B.%20Buravkova"> L. B. Buravkova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work studied the ability of adipose tissue-derived mesenchymal stromal cells (MSCs) to form stroma for expansion of cord blood hematopoietic cells. We showed that 72-hour interaction of MSCs with cord blood mononuclear cells (MNCs) in vitro at atmospheric (20%) and low (5%) O2 conditions increased the expression of ICAM-1, HCAM (at the beginning of interaction) on MSCs. Viability of MSCs and MNCs were maintained at high level. Adhesion of MNCs to MSCs was faster at 20% O2. MSCs promoted the proliferation of adhered MNCs to form the suspension containing great number of hematopoietic colony-forming units, and this effect was more pronounced at 5% O2. Thus, adipose-derived MSCs supplied sufficient stromal support to cord blood MNCs both at 20% and 5% О2, providing their adhesion with further expansion of new generation of different hematopoietic lineages. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hematopoietic%20stem%20and%20progenitor%20cells" title="hematopoietic stem and progenitor cells">hematopoietic stem and progenitor cells</a>, <a href="https://publications.waset.org/abstracts/search?q=mesenchymal%20stromal%20cells" title=" mesenchymal stromal cells"> mesenchymal stromal cells</a>, <a href="https://publications.waset.org/abstracts/search?q=tissue-related%20oxygen" title=" tissue-related oxygen"> tissue-related oxygen</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose%20tissue" title=" adipose tissue"> adipose tissue</a> </p> <a href="https://publications.waset.org/abstracts/13129/stroma-providing-activity-of-adipose-derived-mesenchymal-stromal-cells-in-tissue-related-o2-microenvironment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13129.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">418</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4404</span> Epigenetics Regulation Play Role in the Pathogenesis of Adipose Tissue Disorder, Lipedema</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Musarat%20Ishaq">Musarat Ishaq</a>, <a href="https://publications.waset.org/abstracts/search?q=Tara%20Karnezis"> Tara Karnezis</a>, <a href="https://publications.waset.org/abstracts/search?q=Ramin%20Shayan"> Ramin Shayan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lipedema, a poorly understood chronic disease of adipose hyper-deposition, is often mistaken for obesity and causes significant impairment to mobility and quality-of-life. To identify molecular mechanisms underpinning lipedema, we employed comprehensive omics-based comparative analyses of whole tissue, adipocyte precursors (adipose-derived stem cells (ADSCs)), and adipocytes from patients with or without lipedema. Transcriptional profiling revealed significant differences in lipedema tissue, adipocytes, and ADSCs, with altered levels of mRNAs involved inproliferation and cell adhesion. One highly up-regulated gene in lipedema adipose tissue, adipocytes and ADSCs, ZIC4, encodes Zinc Finger Protein ZIC 4, a class of transcription factor which may be involved in regulating metabolism and adipogenesis. ZIC4 inhibition impaired the adipogenesis of ADSCs into mature adipocytes. Epigenetic regulation study revealed overexpression of ZIC4 is involved in decreased promoter DNA methylation and subsequent decrease in adipogenesis. These epigenetic modifications can alter adipocytes microenvironment and adipocytes differentiation. Our study show that epigenetic events regulate the ability of ADSCs to commit and differentiate into mature adipocytes by modulating ZIC4. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lipedema" title="lipedema">lipedema</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose-derived%20stem%20cells" title=" adipose-derived stem cells"> adipose-derived stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose%20tisue" title=" adipose tisue"> adipose tisue</a>, <a href="https://publications.waset.org/abstracts/search?q=adipocytes" title=" adipocytes"> adipocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=zinc%20finger%20protein" title=" zinc finger protein"> zinc finger protein</a>, <a href="https://publications.waset.org/abstracts/search?q=epigenetic" title=" epigenetic"> epigenetic</a> </p> <a href="https://publications.waset.org/abstracts/143812/epigenetics-regulation-play-role-in-the-pathogenesis-of-adipose-tissue-disorder-lipedema" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143812.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">175</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4403</span> Regulation on Macrophage and Insulin Resistance after Aerobic Exercise in High-Fat Diet Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Qiaofeng%20Guo">Qiaofeng Guo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aims: Obesity is often accompanied by insulin resistance (IR) and whole-body inflammation. Aerobic exercise is an effective treatment to improve insulin resistance and inflammation. However, the anti-inflammatory mechanisms of exercise on epididymal and subcutaneous adipose remain to be elucidated. Here, we compared the macrophage polarization between epididymal and subcutaneous adipose after aerobic exercise. Methods: Male C57BL/6 mice were fed a normal diet group or a high-fat diet group for 12 weeks and performed aerobic training on a treadmill at 55%~65% VO₂ max for eight weeks. Food intake, body weight, and fasting blood glucose levels were monitored weekly. The intraperitoneal glucose tolerance test was to evaluate the insulin resistance model. Fat mass, blood lipid profile, serum IL-1β, TNF-α levels, and CD31/CD206 rates were analysed after the intervention. Results: FBG (P<0.01), AUCIPGTT (P<0.01), and HOMA-IR (P<0.01) increased significantly for a high-fat diet and decreased significantly after the exercise. Eight weeks of aerobic exercise attenuated HFD-induced weight gain and glucose intolerance and improved insulin sensitivity. Serum IL-1β, TNF-α, CD11C/CD206 expression in subcutaneous adipose tissue were not changed before and after exercise, but not in epididymal adipose tissue (P<0.01). Conclusion: Insulin resistance is not accompanied by chronic inflammation and M1 polarization of subcutaneous adipose tissue macrophages in high-fat diet mice. Aerobic exercise effectively improved lipid metabolism and insulin sensitivity, which may be closely associated with reduced M1 polarization of epididymal adipose macrophages. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aerobic%20exercise" title="aerobic exercise">aerobic exercise</a>, <a href="https://publications.waset.org/abstracts/search?q=insulin%20resistance" title=" insulin resistance"> insulin resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=chronic%20inflammation" title=" chronic inflammation"> chronic inflammation</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose" title=" adipose"> adipose</a>, <a href="https://publications.waset.org/abstracts/search?q=macrophage%20polarization" title=" macrophage polarization"> macrophage polarization</a> </p> <a href="https://publications.waset.org/abstracts/161042/regulation-on-macrophage-and-insulin-resistance-after-aerobic-exercise-in-high-fat-diet-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/161042.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">78</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4402</span> Analysis of Adipose Tissue-Derived Mesenchymal Stem Cells under Atherosclerosis Microenvironment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Do%20Khanh%20Vy">Do Khanh Vy</a>, <a href="https://publications.waset.org/abstracts/search?q=Vuong%20Cat%20Khanh"> Vuong Cat Khanh</a>, <a href="https://publications.waset.org/abstracts/search?q=Osamu%20Ohneda"> Osamu Ohneda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> During atherosclerosis (AS) progression, perivascular adipose tissue-derived mesenchymal stem cells (PVAT-MSCs) are exposed to the hypoxic environment due to the oxygenic deprivation which might influence the adipose tissue-derived mesenchymal stem cells (AT-MSCs) function. Additionally, it has been reported that the angiogenic ability of subcutaneous AT-MSCs (SAT-MSCs) was impaired in the AS patients. However, up to now, the effects of AS on the characteristics and function of PVAT-MSCs have not been clarified yet. In the present study, we analyzed the AS microenvironment effects on the characteristics and function of AT-MSCs. We found that there was no significant difference in cellular morphology and differentiation ability between SAT-MSCs and PVAT-MSCs in AS patients. However, the proliferation of AS-derived PVAT-MSCs was less than those of AS-derived SAT-MSCs. Importantly, the migration of AS-derived PVAT-MSCs was faster than AS-derived SAT-MSCs. Of note, AS-derived PVAT-MSCs showed the upregulation of SDF1, which is related to the homing, and VEGF, which is related to the angiogenesis compared to those of AS-derived SAT-MSCs. Consistent with these results, AS-derived PVAT-MSCs showed the higher ability to recruit EPCs and ECs than AS-derived SAT-MSCs. In addition, EPCs and ECs which cultured in the presence of AS-derived PVAT-MSC conditioned medium showed the higher angiogenic function of the tube formation compared to those cultured in AS-derived SAT-MSC conditioned medium. This result suggests that the higher paracrine effects of AS-derived PVAT-MSCs support the angiogenic function of the target cells. Our data showed the different characteristics and functions of AT-MSCs derived from different sources of tissues. Under the AS microenvironment, it seems that the characteristics and functions of PVAT-MSCs might reflect the progression of AS. Further study will be necessary to clarify the mechanism in the future. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=atherosclerosis" title="atherosclerosis">atherosclerosis</a>, <a href="https://publications.waset.org/abstracts/search?q=mesenchymal%20stem%20cells" title=" mesenchymal stem cells"> mesenchymal stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=perivascular%20adipose%20tissue" title=" perivascular adipose tissue"> perivascular adipose tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=subcutaneous%20adipose%20tissue" title=" subcutaneous adipose tissue"> subcutaneous adipose tissue</a> </p> <a href="https://publications.waset.org/abstracts/101561/analysis-of-adipose-tissue-derived-mesenchymal-stem-cells-under-atherosclerosis-microenvironment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/101561.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">161</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4401</span> Morphological Evaluation of Mesenchymal Stem Cells Derived from Adipose Tissue of Dog Treated with Different Concentrations of Nano-Hydroxy Apatite</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Barbaro">K. Barbaro</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Di%20Egidio"> F. Di Egidio</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Amaddeo"> A. Amaddeo</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Lupoli"> G. Lupoli</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Eramo"> S. Eramo</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Barraco"> G. Barraco</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Amaddeo"> D. Amaddeo</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Gallottini"> C. Gallottini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, we wanted to evaluate the effects of nano-hydroxy apatite (NHA) on mesenchymal stem cells extracted from subcutaneous adipose tissue of the dog. The stem cells were divided into 6 experimental groups at different concentrations of NHA. The comparison was made with a control group of stem cell grown in standard conditions without NHA. After 1 week, the cells were fixed with 10% buffered formalin for 1 hour at room temperature and stained with Giemsa, measured at the inverted optical microscope. The morphological evaluation of the control samples and those treated showed that stem cells adhere to the substrate and proliferate in the presence of nanohydroxy apatite at different concentrations showing no detectable toxic effects. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nano-hydroxy%20apatite" title="nano-hydroxy apatite">nano-hydroxy apatite</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose%20mesenchymal%20stem%20cells" title=" adipose mesenchymal stem cells"> adipose mesenchymal stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=dog" title=" dog"> dog</a>, <a href="https://publications.waset.org/abstracts/search?q=morphological%20evaluation" title=" morphological evaluation"> morphological evaluation</a> </p> <a href="https://publications.waset.org/abstracts/12800/morphological-evaluation-of-mesenchymal-stem-cells-derived-from-adipose-tissue-of-dog-treated-with-different-concentrations-of-nano-hydroxy-apatite" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12800.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">473</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4400</span> Burn/Traumatic Scar Maturation Using Autologous Fat Grafts + SVF</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ashok%20K.%20Gupta">Ashok K. Gupta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Over the past few decades, since the bio-engineering revolution, autologous cell therapy (ACT) has become a rapidly evolving field. Currently, this form of therapy has broad applications in modern medicine and plastic surgery, ranging from the treatment/improvement of wound healing to life-saving operations. A study was conducted on 50 patients having to disfigure, and deform post burn scars and was treated by injection of extracted, refined adipose tissue grafts with their unique stem cell properties. To compare the outcome, a control of 20 such patients was treated with conventional skin or soft-tissue flaps or skin grafting, and a control of 10 was treated with more advanced microsurgical techniques such as Pre-fabricated flaps/pre laminated flaps / free flaps. Assessment of fat volume and survival post- follow up period was done by radiological aid, using MRI and clinically (Survival of the autograft and objective parameters for scar elasticity were evaluated skin elasticity parameters 3 to 9 months postoperatively). Recently, an enzyme that is involved in collagen crosslinking in fibrotic tissue, lysyl hydroxylase (LH2), was identified. This enzyme is normally active in bone and cartilage but hardly in the skin. It has been found that this enzyme is highly expressed in scar tissue and subcutaneous fat; this is in contrast to the dermis, where the enzyme is hardly expressed. Adipose tissue-derived stem cell injections are an effective method in the treatment of various extensive post-burn scar deformities that makes it possible to re-create the lost sub-dermal tissue for improvement in the function of involved joint movements. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adipose%20tissue-derived%20stem%20cell%20injections" title="adipose tissue-derived stem cell injections">adipose tissue-derived stem cell injections</a>, <a href="https://publications.waset.org/abstracts/search?q=treatment%20of%20various%20extensive%20post-burn%20scar%20deformities" title=" treatment of various extensive post-burn scar deformities"> treatment of various extensive post-burn scar deformities</a>, <a href="https://publications.waset.org/abstracts/search?q=re-create%20the%20lost%20sub-dermal%20tissue" title=" re-create the lost sub-dermal tissue"> re-create the lost sub-dermal tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=improvement%20in%20function%20of%20involved%20joint%20movements" title=" improvement in function of involved joint movements"> improvement in function of involved joint movements</a> </p> <a href="https://publications.waset.org/abstracts/164272/burntraumatic-scar-maturation-using-autologous-fat-grafts-svf" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164272.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">67</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4399</span> A Secreted Protein Can Attenuate High Fat Diet Induced Obesity and Metabolic Syndrome in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdul%20Soofi">Abdul Soofi</a>, <a href="https://publications.waset.org/abstracts/search?q=Katherine%20Wolf"> Katherine Wolf</a>, <a href="https://publications.waset.org/abstracts/search?q=Egon%20Ranghini"> Egon Ranghini</a>, <a href="https://publications.waset.org/abstracts/search?q=Gregory%20Dressler"> Gregory Dressler</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Obesity and its associated complications, such as insulin resistance and non-alcoholic fatty liver disease, are reaching epidemic proportions. In mice, the TGF-β superfamily is implicated in the regulation of white and brown adipose tissues differentiation. The Kielin/Chordin-like Protein (KCP) is a secreted regulator of the TGF-β superfamily pathways that can inhibit both TGF-β and Activin signals while enhancing the Bone Morphogenetic protein (BMP) signaling. However, the effects of KCP on metabolism and obesity have not been studied in animal models. Thus, we examined the effects of KCP loss or gain of function in mice that were maintained on either a regular or a high fat diet. Loss of KCP sensitized mice to obesity and associated complications such as hepatic steatosis and glucose intolerance. In contrast, transgenic mice that expressed KCP in the kidney, liver and adipose tissues were resistant to developing high fat diet induced obesity and had significantly reduced white adipose tissue. KCP over-expression was able to shift the pattern of Smad signaling in vivo, to increase the levels of P-Smad1 and decrease P-Smad3, resulting in resistance to high fat diet induced hepatic steatosis and glucose intolerance. In aging mice, loss of KCP promoted liver pathology even when mice were fed a normal diet. The data demonstrate that shifting the TGF-β superfamily signaling with a secreted inhibitor or enhancer can alter the physiology of adipose tissue to reduce obesity and can inhibit the initiation and progression of hepatic steatosis to significantly reduce the effects of high fat diet induced metabolic disease. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adipose%20tissue" title="adipose tissue">adipose tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=KCP" title=" KCP"> KCP</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=TGF-%CE%B2" title=" TGF-β"> TGF-β</a>, <a href="https://publications.waset.org/abstracts/search?q=BMP" title=" BMP"> BMP</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatic%20steatosis" title=" hepatic steatosis"> hepatic steatosis</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolic%20syndrome" title=" metabolic syndrome"> metabolic syndrome</a> </p> <a href="https://publications.waset.org/abstracts/38090/a-secreted-protein-can-attenuate-high-fat-diet-induced-obesity-and-metabolic-syndrome-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/38090.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">353</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4398</span> Contour Defects of Face with Hyperpigmentation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Afzaal%20Bashir">Afzaal Bashir</a>, <a href="https://publications.waset.org/abstracts/search?q=Sunaina%20Afzaal"> Sunaina Afzaal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Facial contour deformities associated with pigmentary changes are of major concern for plastic surgeons, both being important and difficult in treating such issues. No definite ideal treatment option is available to simultaneously address both the contour defect as well as related pigmentation. Objectives: The aim of the current study is to compare the long-term effects of conventional adipose tissue grafting and ex-vivo expanded Mesenchymal Stem Cells enriched adipose tissue grafting for the treatment of contour deformities with pigmentary changes on the face. Material and Methods: In this study, eighty (80) patients with contour deformities of the face with hyperpigmentation were recruited after informed consent. Two techniques i.e., conventional fat grafting (C-FG) and fat grafts enriched with expanded adipose stem cells (FG-ASCs), were used to address the pigmentation. Both techniques were explained to patients, and enrolled patients were divided into two groups i.e., C-FG and FG-ASCS, per patients’ choice and satisfaction. Patients of the FG-ASCs group were treated with fat grafts enriched with expanded adipose stem cells, while patients of the C-FGs group were treated with conventional fat grafting. Patients were followed for 12 months, and improvement in face pigmentation was assessed clinically as well as measured objectively. Patient satisfaction was also documented as highly satisfied, satisfied, and unsatisfied. Results: Mean age of patients was 24.42(±4.49), and 66 patients were females. The forehead was involved in 61.20% of cases, the cheek in 21.20% of cases, the chin in 11.20% of cases, and the nose in 6.20% of cases. In the GF-ASCs group, the integrated color density (ICD) was decreased (1.08×10⁶ ±4.64×10⁵) as compared to the C-FG group (2.80×10⁵±1.69×10⁵). Patients treated with fat grafts enriched with expanded adipose stem cells were significantly more satisfied as compared to patients treated with conventional fat grafting only. Conclusion: Mesenchymal stem cell-enriched autologous fat grafting is a preferred option for improving the contour deformities related to increased pigmentation of face skin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hyperpigmentation" title="hyperpigmentation">hyperpigmentation</a>, <a href="https://publications.waset.org/abstracts/search?q=color%20density" title=" color density"> color density</a>, <a href="https://publications.waset.org/abstracts/search?q=enriched%20adipose%20tissue%20graft" title=" enriched adipose tissue graft"> enriched adipose tissue graft</a>, <a href="https://publications.waset.org/abstracts/search?q=fat%20grafting" title=" fat grafting"> fat grafting</a>, <a href="https://publications.waset.org/abstracts/search?q=contour%20deformities" title=" contour deformities"> contour deformities</a>, <a href="https://publications.waset.org/abstracts/search?q=Image%20J" title=" Image J"> Image J</a> </p> <a href="https://publications.waset.org/abstracts/162936/contour-defects-of-face-with-hyperpigmentation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162936.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">110</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4397</span> 12-Week Comparative Clinical Trial with Low Dose Phentermine/Topiramate with Liraglutide on Obesity in Korea</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kyu%20Rae%20Lee">Kyu Rae Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of the study is to investigate the clinical efficacy of combination therapeutic modalities using liraglutide (1.2mg/d) add on low-dose phentermine (7.5 mg/d)/topiramate (50mg/d) medication on the obese patient in the bariatric clinic. We assessed the retrospective cohort clinical analyses to the clinical efficacy of medication and combination in the patients who visited the bariatric clinic. We measured all participants’ body fat (bioelectric impedance analysis), weight, height, and the cross-sectional areas of adipose tissues (umbilicus level) after keep fasting for 8 hours at 0, 4, 12 weeks. The design of the study was opened, paired t-test and Wilcoxon test were performed using SPSS for windows (ver.18, IL, USA) for comparison of weight, body fat, and adipose tissues. The participants were one hundred twenty-eight subjects aged 44.67 (1.18) years, 28.95 (0.39) kg/m², and female (82.7%). Their body fat was 40.57 (2.23%), and waist to hip ratio was 0.96 (0.01). The mean cross-sectional area of visceral adipose tissue was 142.59 (7.06) mm², and that of subcutaneous adipose was 274.37 (9.18) mm². 73 of them (57.5%) took medication only, 54 of them took medication with liraglutide for 12 weeks. The subjects in the medication group lost 5.4165 kg, 6.8069%, and those of the combination group did 6.2481 kg, 3.564%. The mean cross-sectional areas of visceral, subcutaneous adipose tissue in the medication group significantly decreased (p=.043), even more in the combination group. (p=.028). Further controlled clinical trials should be considered in the future. We conclude that the low dose of phentermine/topiramate with liraglutide therapeutic modalities would be more effective than phentermine/topiramate medication only in obesity treatment for 12 weeks. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=low%20dose%20phentermine" title="low dose phentermine">low dose phentermine</a>, <a href="https://publications.waset.org/abstracts/search?q=topiramate" title=" topiramate"> topiramate</a>, <a href="https://publications.waset.org/abstracts/search?q=liraglutide" title=" liraglutide"> liraglutide</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=efficacy" title=" efficacy"> efficacy</a> </p> <a href="https://publications.waset.org/abstracts/145671/12-week-comparative-clinical-trial-with-low-dose-phenterminetopiramate-with-liraglutide-on-obesity-in-korea" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/145671.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">158</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4396</span> The Cut-Off Value of TG/HDL Ratio of High Pericardial Adipose Tissue</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nam-Seok%20Joo">Nam-Seok Joo</a>, <a href="https://publications.waset.org/abstracts/search?q=Da-Eun%20Jung"> Da-Eun Jung</a>, <a href="https://publications.waset.org/abstracts/search?q=Beom-Hee%20Choi"> Beom-Hee Choi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background and Objectives: Recently, the triglyceride/high-density lipoprotine cholesterol (TG/HDL) ratio and pericardial adipose tissue (PAT) has gained attention as an indicator related to metabolic syndrome (MS). To date, there has been no research on the relationship between TG/HDL and PAT, we aimed to investigate the association between the TG/HDL and PAT. Methods: In this cross-sectional study, we investigated 627 patients who underwent coronary multidetector computed tomography and metabolic parameters. We divided subjects into two groups according to the cut-off PAT volume associated with MS, which is 142.2 cm³, and we compared metabolic parameters between those groups. We divided the TG/HDL ratio into tertiles according to Log(TG/HDL) and compared PAT-related parameters by analysis of variance. Finally, we applied logistic regression analysis to obtain the odds ratio of high PAT (PAT volume≥142.2 cm³) in each tertile, and we performed receiver operating characteristic (ROC) analysis to get the cut-off of TG/HDL ratio according to high PAT. Results: The mean TG/ HDL ratio of the high PAT volume group was 3.6, and TG/ HDL ratio had a strong positive correlation with various metabolic parameters. In addition, in the Log (TG/HDL) tertile groups, the higher tertile had more metabolic derangements, including PAT, and showed higher odds ratios of having high PAT (OR=4.10 in the second tertile group and OR=5.06 in their third tertile group, respectively) after age, sex, smoking adjustments. TG/HDL ratio according to the having increased PAT by ROC curve showed 1.918 (p < 0.001). Conclusion: TG/HDL ratio and high PAT volume have a significant positive correlation, and higher TG/HDL ratio showed high PAT. The cut-off value of the TG/HDL ratio was 1.918 to have a high PAT. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=triglyceride" title="triglyceride">triglyceride</a>, <a href="https://publications.waset.org/abstracts/search?q=high-density%20lipoprotein" title=" high-density lipoprotein"> high-density lipoprotein</a>, <a href="https://publications.waset.org/abstracts/search?q=pericardial%20adipose%20tissue" title=" pericardial adipose tissue"> pericardial adipose tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=cut-off%20value" title=" cut-off value"> cut-off value</a> </p> <a href="https://publications.waset.org/abstracts/192981/the-cut-off-value-of-tghdl-ratio-of-high-pericardial-adipose-tissue" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/192981.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">15</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4395</span> Effects of Pterostilbene in Brown Adipose Tissue from Obese Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Leixuri%20Aguirre">Leixuri Aguirre</a>, <a href="https://publications.waset.org/abstracts/search?q=I%C3%B1aki%20Milton-Laskibar"> Iñaki Milton-Laskibar</a>, <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20Hijona"> Elizabeth Hijona</a>, <a href="https://publications.waset.org/abstracts/search?q=Luis%20Bujanda"> Luis Bujanda</a>, <a href="https://publications.waset.org/abstracts/search?q=Agnes%20M.%20Rimando"> Agnes M. Rimando</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20P.%20Portillo"> Maria P. Portillo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: In recent years great attention has been paid by scientific community to phenolic compounds as active biomolecules naturally present in foodstuffs due to their beneficial effects on health. Pterostilbene is a resveratrol dimethylether derivative which shows higher biodisponibility. Objective. To analyze the effects of two doses of pterostilbene on several markers of thermogenic capacity in a model of genetic obesity, which shows reduced thermogenesis. Methods: The experiment was conducted with thirty Zucker (fa/fa) rats that were distributed in 3 experimental groups, the control group and two groups orally administered with pterostilbene at 15 and 30 mg/kg body weight/day for 6 weeks. Gene expression of Ucp1, Pgc-1α, Cpt1b, Pparα, Nfr1, Tfam and Cox-2 were assessed by RT-PCR, protein expression of UCP1 and GLUT4 by western blot and enzyme activity of carnitine palmitoyl transferase 1b and citrate synthase by spectrophotometry in interscapular brown adipose tissue (iBAT). Statistical analysis was performed by using one way ANOVA and Newman-Keuls as post-hoc test. Results: Pterostilbene did not change gene expression of Pgc-1α. However, significant increases were found in the expression of Ucp1, Pparα, Nfr-1 and Cox-2. Protein expression of UCP1 and GLUT4 was increased in animals treated with pterostilbene, as well as the activities of CPT-1b and CS. These effects were observed with both doses of pterostilbene, without differences between them. Conclusions: These results show that pterostilbene increases thermogenic and oxidative capacity of brown adipose tissue in obese rats. Whether these effects effectively contribute to the anti-obesity properties of these compound needs further research. Acknowledgments: MINECO-FEDER (AGL2015-65719-R), Basque Government (IT-572-13), University of the Basque Country (ELDUNANOTEK UFI11/32), Institut of Health Carlos III (CIBERobn). Iñaki Milton is a fellowship from the Basque Government. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=brown%20adipose%20tissue" title="brown adipose tissue">brown adipose tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=pterostilbene" title=" pterostilbene"> pterostilbene</a>, <a href="https://publications.waset.org/abstracts/search?q=thermogenesis" title=" thermogenesis"> thermogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=uncoupling%20protein%201" title=" uncoupling protein 1"> uncoupling protein 1</a> </p> <a href="https://publications.waset.org/abstracts/61153/effects-of-pterostilbene-in-brown-adipose-tissue-from-obese-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61153.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">297</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4394</span> Implementation of Autologous Adipose Graft from the Abdomen for Complete Fat Pad Loss of the Heel Following a Traumatic Open Fracture Secondary to a Motor Vehicle Accident: A Case Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ahmad%20Saad">Ahmad Saad</a>, <a href="https://publications.waset.org/abstracts/search?q=Shuja%20Abbas"> Shuja Abbas</a>, <a href="https://publications.waset.org/abstracts/search?q=Breanna%20Marine"> Breanna Marine</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: This study explores the potential applications of autologous pedal fat pad grafting as a minimally invasive therapeutic strategy for addressing pedal fat pad loss. Without adequate shock absorbing tissue, a patient can experience functional deficits, ulcerations, loss of quality of life, and significant limitations with ambulation. This study details a novel technique involving autologous adipose grafting from the abdomen to enhance plantar fat pad thickness in a patient involved in a severe motor vehicle accident which resulted in total fat pad loss of the heel. Autologous adipose grafting (AAG) was used following adipose allografting in an effort to recreate a normal shock absorbing surface to allow return to activities of daily living and painless ambulation. Methods: A 46-year-old male sustained multiple open pedal fractures and necrosis to the heel fat pad after a motorcycle accident, which resulted in complete loss of the calcaneal fat pad. The patient underwent serial debridement’s, utilization of wound vac therapy and split thickness skin grafting to accomplish complete closure, despite complete loss of adipose to area. Patient presented with complaints of pain on ambulation, inability to bear weight on the heel, recurrent ulcerations, admitted had not been ambulating for two years. Clinical exam demonstrated complete loss of the plantar fat pad with a thin layer of epithelial tissue overlying the calcaneal bone, allowing visibility of the osseous contour of the calcaneus. Scar tissue had formed in place of the fat pad, with thickened epithelial tissue extending from the midfoot to the calcaneus. After conservative measures were exhausted, the patient opted for initial management by adipose allograft matrix (AAM) injections. Post operative X-ray imaging revealed noticeable improvement in calcaneal fat pad thickness. At 1 year follow up, the patient was able to ambulate without assistive devices. The fat pad at this point was significantly thicker than it was pre-operatively, but the thickness did not restore to pre-accident thickness. In order to compare the take of allograft versus autografting of adipose tissue, the decision to use adipose autograft through abdominal liposuction harvesting was deemed suitable. A general surgeon completed harvesting of adipose cells from the patient’s abdomen via liposuction, and a podiatric surgeon performed the AAG injection into the heel. Total of 15 cc’s of autologous adipose tissue injected to the calcaneus. Results: There was a visual increase in the calcaneal fat pad thickness both clinically and radiographically. At the 6-week follow up, imaging revealed retention of the calcaneal fat pad thickness. Three months postop, patient returned to activities of daily living and increased quality of life due to their increased ability to ambulate. Discussion: AAG is a novel treatment for pedal fat pad loss. These treatments may be viable and reproducible therapeutic choices for patients suffering from fat pad atrophy, fat pad loss, and/or plantar ulcerations. Both treatments of AAM and AAG exhibited similar therapeutic results by providing pain relief for ambulation and allowing for patients to return to their quality of life. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=podiatry" title="podiatry">podiatry</a>, <a href="https://publications.waset.org/abstracts/search?q=wound" title=" wound"> wound</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose" title=" adipose"> adipose</a>, <a href="https://publications.waset.org/abstracts/search?q=allograft" title=" allograft"> allograft</a>, <a href="https://publications.waset.org/abstracts/search?q=autograft" title=" autograft"> autograft</a>, <a href="https://publications.waset.org/abstracts/search?q=wound%20care" title=" wound care"> wound care</a>, <a href="https://publications.waset.org/abstracts/search?q=limb%20reconstruction" title=" limb reconstruction"> limb reconstruction</a>, <a href="https://publications.waset.org/abstracts/search?q=injection" title=" injection"> injection</a>, <a href="https://publications.waset.org/abstracts/search?q=limb%20salvage" title=" limb salvage"> limb salvage</a> </p> <a href="https://publications.waset.org/abstracts/170743/implementation-of-autologous-adipose-graft-from-the-abdomen-for-complete-fat-pad-loss-of-the-heel-following-a-traumatic-open-fracture-secondary-to-a-motor-vehicle-accident-a-case-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/170743.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">82</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4393</span> Advancement in Adhesion and Osteogenesis of Stem Cells with Histatin Coated 3D-Printed Bio-Ceramics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Haiyan%20Wang">Haiyan Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Dongyun%20Wang"> Dongyun Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yongyong%20Yan"> Yongyong Yan</a>, <a href="https://publications.waset.org/abstracts/search?q=Richard%20T.%20Jaspers"> Richard T. Jaspers</a>, <a href="https://publications.waset.org/abstracts/search?q=Gang%20Wu"> Gang Wu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mesenchymal stem cell and 3D printing-based bone tissue engineering present a promising technique to repair large-volume bone defects. Its success is highly dependent on cell attachment, spreading, osteogenic differentiation, and in vivo survival of stem cells on 3D-printed scaffolds. In this study, human salivary histatin-1 (Hst1) was utilized to enhance the interactions between human adipose-derived stem cells (hASCs) and 3D-printed β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Fluorescent images showed that Hst1 significantly enhanced the adhesion of hASCs to both bioinert glass and 3D-printed β-TCP scaffold. In addition, Hst1 was associated with significantly higher proliferation and osteogenic differentiation of hASCs on 3D-printed β-TCP scaffolds. Moreover, coating 3D-printed β-TCP scaffolds with histatin significantly promotes the survival of hASCs in vivo. The ERK and p38 but not JNK signaling was found to be involved in the superior adhesion of hASCs to β-TCP scaffolds with the aid of Hst1. In conclusion, Hst1 could significantly promote the adhesion, spreading, osteogenic differentiation, and in vivo survival of hASCs on 3D-printed β-TCP scaffolds, bearing a promising application in stem cell/3D printing-based constructs for bone tissue engineering. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=3d%20printing" title="3d printing">3d printing</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose-derived%20stem%20cells" title=" adipose-derived stem cells"> adipose-derived stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=bone%20tissue%20engineering" title=" bone tissue engineering"> bone tissue engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=histatin-1" title=" histatin-1"> histatin-1</a>, <a href="https://publications.waset.org/abstracts/search?q=osteogenesis" title=" osteogenesis"> osteogenesis</a> </p> <a href="https://publications.waset.org/abstracts/183798/advancement-in-adhesion-and-osteogenesis-of-stem-cells-with-histatin-coated-3d-printed-bio-ceramics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/183798.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">63</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4392</span> Septin 11, Cytoskeletal Protein Involved in the Regulation of Lipid Metabolism in Adipocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Moreno-Castellanos">Natalia Moreno-Castellanos</a>, <a href="https://publications.waset.org/abstracts/search?q=Amaia%20Rodriguez"> Amaia Rodriguez</a>, <a href="https://publications.waset.org/abstracts/search?q=Gema%20Fr%C3%BChbeck"> Gema Frühbeck</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: In adipocytes, the cytoskeleton undergoes important expression and distribution in adipocytes rearrangements during adipogenesis and in obesity. Indeed, a role for these proteins in the regulation of adipocyte differentiation and response to insulin has been demonstrated. Recently, septins have been considered as new components of the cytoskeletal network that interact with other cytoskeletal elements (actin and tubulin) profoundly modifying their dynamics. However, these proteins have not been characterized as yet in adipose tissue. In this work, were examined the cellular, molecular and functional features of a member of this family, septin 11 (SEPT11), in adipocytes and evaluated the impact of obesity on the expression of this protein in human adipose tissue. Methods: Adipose gene and protein expression levels of SEPT11 were analysed in human samples. SEPT11 distribution was evaluated by immunocytochemistry, electronic microscopy, and subcellular fractionation techniques. GST-pull down, immunoprecipitation and a Yeast-Two Hybrid (Y2H) screening were used to identify the SEPT11 interactome. Gene silencing was employed to assess the role of SEPT11 in the regulation of insulin signaling and lipid metabolism in adipocytes. Results: SEPT11 is expressed in human adipocytes, and its levels increased in both omental and subcutaneous adipose tissue in obesity, with SEPT11 mRNA content positively correlating with parameters of insulin resistance in subcutaneous fat. In non-stimulated adipocytes, SEPT11 immunoreactivity showed a ring-like distribution at the cell surface and associated to caveolae. Biochemical analyses showed that SEPT11 interacted with the main component of caveolae, caveolin-1 (CAV1) as well as with the fatty acid-binding protein, FABP5. Notably, the three proteins redistributed and co-localized at the surface of lipid droplets upon exposure of adipocytes to oleate. In this line, SEPT11 silencing in 3T3-L1 adipocytes impaired insulin signaling and decreased insulin-induced lipogenesis. Conclusions: Those findings demonstrate that SEPT11 is a novel component of the adipocyte cytoskeleton that plays an important role in the regulation of lipid traffic, metabolism and can thus represent a potential biomarker of insulin resistance in obesity in adipocytes through its interaction with both CAV1 and FABP5. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=caveolae" title="caveolae">caveolae</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20metabolism" title=" lipid metabolism"> lipid metabolism</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=septins" title=" septins"> septins</a> </p> <a href="https://publications.waset.org/abstracts/79045/septin-11-cytoskeletal-protein-involved-in-the-regulation-of-lipid-metabolism-in-adipocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/79045.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">214</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4391</span> Effect of Dietary Supplementation of Allium Hookeri Root and Processed Sulfur on the Growth Performance of Guinea Pigs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nayeon">Nayeon</a>, <a href="https://publications.waset.org/abstracts/search?q=Lee"> Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Won-Young"> Won-Young</a>, <a href="https://publications.waset.org/abstracts/search?q=Cho"> Cho</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyun%20Joo"> Hyun Joo</a>, <a href="https://publications.waset.org/abstracts/search?q=Jang"> Jang</a>, <a href="https://publications.waset.org/abstracts/search?q=Chi-Ho"> Chi-Ho</a>, <a href="https://publications.waset.org/abstracts/search?q=Lee"> Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study investigated the effects of the dietary supplementation of the <em>Allium hookeri </em>root, and processed sulfur, on the growth performance of guinea pigs. The guinea pigs were fed a control diet (CON), as well as the control diet including 1% freeze-dried <em>Allium hookeri </em>root (AH), or 0.1% processed sulfur (S), or including both the freeze-dried <em>Allium hookeri </em>root and the processed sulfur (AHS). The weight of perirenal adipose tissue (PAT) and the epididymal adipose tissue (EAT) in the AH were significantly lower than CON (p < 0.05). The serum cholesterols levels of the AH and the AHS were significantly lower than the S (p < 0.05). While the total saturated fatty acid content in the serum of the AH and AHS groups showed a tendency to decrease, the total monounsaturated fatty acid increased. The results of this study suggested that dietary consumption of <em>Allium hookeri</em> root may help to decrease fat accumulation, lower serum cholesterol levels, and control serum free fatty acid contents in the guinea pigs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Allium%20hookeri" title="Allium hookeri">Allium hookeri</a>, <a href="https://publications.waset.org/abstracts/search?q=dietary%20supplementation" title=" dietary supplementation"> dietary supplementation</a>, <a href="https://publications.waset.org/abstracts/search?q=growth%20performance" title=" growth performance"> growth performance</a>, <a href="https://publications.waset.org/abstracts/search?q=processed%20sulfur" title=" processed sulfur"> processed sulfur</a>, <a href="https://publications.waset.org/abstracts/search?q=Guinea%20pig" title=" Guinea pig"> Guinea pig</a> </p> <a href="https://publications.waset.org/abstracts/77113/effect-of-dietary-supplementation-of-allium-hookeri-root-and-processed-sulfur-on-the-growth-performance-of-guinea-pigs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77113.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">269</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4390</span> Molecular Pathogenesis of NASH through the Dysregulation of Metabolic Organ Network in the NASH-HCC Model Mouse Treated with Streptozotocin-High Fat Diet</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bui%20Phuong%20Linh">Bui Phuong Linh</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuki%20Sakakibara"> Yuki Sakakibara</a>, <a href="https://publications.waset.org/abstracts/search?q=Ryuto%20Tanaka"> Ryuto Tanaka</a>, <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20H.%20Pigney"> Elizabeth H. Pigney</a>, <a href="https://publications.waset.org/abstracts/search?q=Taishi%20Hashiguchi"> Taishi Hashiguchi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> NASH is an increasingly prevalent chronic liver disease that can progress to hepatocellular carcinoma and now is attracting interest worldwide. The STAM™ model is a clinically-correlated murine NASH model which shows the same pathological progression as NASH patients and has been widely used for pharmacological and basic research. The multiple parallel hits hypothesis suggests abnormalities in adipocytokines, intestinal microflora, and endotoxins are intertwined and could contribute to the development of NASH. In fact, NASH patients often exhibit gut dysbiosis and dysfunction in adipose tissue and metabolism. However, the analysis of the STAM™ model has only focused on the liver. To clarify whether the STAM™ model can also mimic multiple pathways of NASH progression, we analyzed the organ crosstalk interactions between the liver and the gut and the phenotype of adipose tissue in the STAM™ model. NASH was induced in male mice by a single subcutaneous injection of 200 µg streptozotocin 2 days after birth and feeding with high-fat diet after 4 weeks of age. The mice were sacrificed at NASH stage. Colon samples were snap-frozen in liquid nitrogen and stored at -80˚C for tight junction-related protein analysis. Adipose tissue was prepared into paraffin blocks for HE staining. Blood adiponectin was analyzed to confirm changes in the adipocytokine profile. Tight junction-related proteins in the intestine showed that expression of ZO-1 decreased with the progression of the disease. Increased expression of endotoxin in the blood and decreased expression of Adiponectin were also observed. HE staining revealed hypertrophy of adipocytes. Decreased expression of ZO-1 in the intestine of STAM™ mice suggests the occurrence of leaky gut, and abnormalities in adipocytokine secretion were also observed. Together with the liver, phenotypes in these organs are highly similar to human NASH patients and might be involved in the pathogenesis of NASH. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Non-alcoholic%20steatohepatitis" title="Non-alcoholic steatohepatitis">Non-alcoholic steatohepatitis</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatocellular%20carcinoma" title=" hepatocellular carcinoma"> hepatocellular carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=fibrosis" title=" fibrosis"> fibrosis</a>, <a href="https://publications.waset.org/abstracts/search?q=organ%20crosstalk" title=" organ crosstalk"> organ crosstalk</a>, <a href="https://publications.waset.org/abstracts/search?q=leaky%20gut" title=" leaky gut"> leaky gut</a> </p> <a href="https://publications.waset.org/abstracts/143590/molecular-pathogenesis-of-nash-through-the-dysregulation-of-metabolic-organ-network-in-the-nash-hcc-model-mouse-treated-with-streptozotocin-high-fat-diet" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143590.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4389</span> Properties of Adipose Tissue Derived Mesenchymal Stem Cells with Long-Term Cryopreservation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jienny%20Lee"> Jienny Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=In-Soo%20Cho"> In-Soo Cho</a>, <a href="https://publications.waset.org/abstracts/search?q=Sang-Ho%20Cha"> Sang-Ho Cha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Adult mesenchymal stem cells (MSCs) have been investigated using preclinical approaches for tissue regeneration. Porcine MSCs (pMSCs) are capable of growing and attaching to plastic with a fibroblast-like morphology and then differentiating into bone, adipose, and cartilage tissues <em>in vitro</em>. This study was conducted to investigate the proliferating abilities, differentiation potentials, and multipotency of miniature pig adipose tissue-derived MSCs (mpAD-MSCs) with or without long-term cryopreservation, considering that cryostorage has the potential for use in clinical applications. After confirming the characteristics of the mpAD-MSCs, we examined the effect of long-term cryopreservation (> 2 years) on expression of cell surface markers (CD34, CD90 and CD105), proliferating abilities (cumulative population doubling level, doubling time, colony-forming unit, and MTT assay) and differentiation potentials into mesodermal cell lineages. As a result, the expression of cell surface markers is similar between thawed and fresh mpAD-MSCs. However, long-term cryopreservation significantly lowered the differentiation potentials (adipogenic, chondrogenic, and osteogenic) of mpAD-MSCs. When compared with fresh mpAD-MSCs, thawed mpAD-MSCs exhibited lower expression of mesodermal cell lineage-related genes such as peroxisome proliferator-activated receptor-g2, lipoprotein lipase, collagen Type II alpha 1, osteonectin, and osteocalcin. Interestingly, long-term cryostoraged mpAD-MSCs exhibited significantly higher cell viability than the fresh mpAD-MSCs. Long-term cryopreservation induced a 30% increase in the cell viability of mpAD-MSCs when compared with the fresh mpAD-MSCs at 5 days after thawing. However, long-term cryopreservation significantly lowered expression of stemness markers such as Oct3/4, Sox2, and Nanog. Furthermore, long-term cryopreservation negatively affected expression of senescence-associated genes such as telomerase reverse transcriptase and heat shock protein 90 of mpAD-MSCs when compared with the fresh mpAD-MSCs. The results from this study might be important for the successful application of MSCs in clinical trials after long-term cryopreservation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mesenchymal%20stem%20cells" title="mesenchymal stem cells">mesenchymal stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=cryopreservation" title=" cryopreservation"> cryopreservation</a>, <a href="https://publications.waset.org/abstracts/search?q=stemness" title=" stemness"> stemness</a>, <a href="https://publications.waset.org/abstracts/search?q=senescence" title=" senescence"> senescence</a> </p> <a href="https://publications.waset.org/abstracts/45070/properties-of-adipose-tissue-derived-mesenchymal-stem-cells-with-long-term-cryopreservation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/45070.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">235</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4388</span> Histological Changes in the Culex pipiens Mosquito Larvae Treated by the Entomopathogenic Fungus Beauveria bassiana</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fatma%20Sahir-%20Halouane">Fatma Sahir- Halouane</a>, <a href="https://publications.waset.org/abstracts/search?q=Sonia%20Hamid"> Sonia Hamid</a>, <a href="https://publications.waset.org/abstracts/search?q=Farida%20Tihar-Benzina"> Farida Tihar-Benzina</a>, <a href="https://publications.waset.org/abstracts/search?q=Fatiha%20Bouhlali"> Fatiha Bouhlali</a>, <a href="https://publications.waset.org/abstracts/search?q=Souad%20Lourchane"> Souad Lourchane</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Culicidae are biting insects, the most harmful to people, they are almost all bloodsuckers, and they are responsible of the spread of many important diseases such as malaria, yellow fever, and elephantiasis. Entomopathogenic microorganisms occupy an important place among the alternative methods of fighting against pests insect. The fungus Beauveria bassiana is an entomopathogenic agent naturally present in the ecosystems. It offers a very interesting potential for controlling populations of mosquitoes. This study aimed to show the histological changes that occured in Culex pipiens larvae infected with Beauveria bassiana. The 4th instar larvae were infected with B. bassiana in 10-7 spore/ml dilution, the histological section was studied showing that the fungi infected all the body parts specially Cuticle, Epiderms, fat bodies and midgut. After then the insect have a white appearance and covered with a thick coat of hyphea. The obtained results show that the application of Beauveria bassiana on cuticle of the fourth stage larvae of Culex pipiens was dependent of an apparent disturbance on the structure of the cuticle or there has been the degeneration of its different parts, infection of the fungus does not stop at the body walls. Therefore, it affects even the Adipose tissue, epidermal cells and intestine. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Culex%20pipiens" title="Culex pipiens">Culex pipiens</a>, <a href="https://publications.waset.org/abstracts/search?q=Beauveria%20bassiana" title=" Beauveria bassiana"> Beauveria bassiana</a>, <a href="https://publications.waset.org/abstracts/search?q=histological%20changes" title=" histological changes"> histological changes</a>, <a href="https://publications.waset.org/abstracts/search?q=cuticle" title=" cuticle"> cuticle</a>, <a href="https://publications.waset.org/abstracts/search?q=intestine%20and%20adipose%20tissue" title=" intestine and adipose tissue "> intestine and adipose tissue </a> </p> <a href="https://publications.waset.org/abstracts/12650/histological-changes-in-the-culex-pipiens-mosquito-larvae-treated-by-the-entomopathogenic-fungus-beauveria-bassiana" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12650.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4387</span> Serum Neurotrophins in Different Metabolic Types of Obesity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Irina%20M.%20Kolesnikova">Irina M. Kolesnikova</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrey%20M.%20Gaponov"> Andrey M. Gaponov</a>, <a href="https://publications.waset.org/abstracts/search?q=Sergey%20A.%20Roumiantsev"> Sergey A. Roumiantsev</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatiana%20V.%20Grigoryeva"> Tatiana V. Grigoryeva</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexander%20V.%20Laikov"> Alexander V. Laikov</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexander%20V.%20Shestopalov"> Alexander V. Shestopalov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background. Neuropathy is a common complication of obesity. In this regard, the content of neurotrophins in such patients is of particular interest. Neurotrophins are the proteins that regulate neuron survival and neuroplasticity and include brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). However, the risk of complications depends on the metabolic type of obesity. Metabolically unhealthy obesity (MUHO) is associated with a high risk of complications, while this is not the case with metabolically healthy obesity (MHO). Therefore, the aim of our work was to study the effect of the obesity metabolic type on serum neurotrophins levels. Patients, materials, methods. The study included 134 healthy donors and 104 obese patients. Depending on the metabolic type of obesity, the obese patients were divided into subgroups with MHO (n=40) and MUHO (n=55). In the blood serum, the concentration of BDNF and NGF was determined. In addition, the content of adipokines (leptin, asprosin, resistin, adiponectin), myokines (irisin, myostatin, osteocrin), indicators of carbohydrate, and lipid metabolism were measured. Correlation analysis revealed the relationship between the studied parameters. Results. We found that serum BDNF concentration was not different between obese patients and healthy donors, regardless of obesity metabolic type. At the same time, in obese patients, there was a decrease in serum NGF level versus control. A similar trend was characteristic of both MHO and MUHO. However, MUHO patients had a higher NGF level than MHO patients. The literature indicates that obesity is associated with an increase in the plasma concentration of NGF. It can be assumed that in obesity, there is a violation of NGF storage in platelets, which accelerates neurotrophin degradation. We found that BDNF concentration correlated with irisin levels in MUHO patients. Healthy donors had a weak association between NGF and VEGF levels. No such association was found in obese patients, but there was an association between NGF and leptin concentrations. In MHO, the concentration of NHF correlated with the content of leptin, irisin, osteocrin, insulin, and the HOMA-IR index. But in MUHO patients, we found only the relationship between NGF and adipokines (leptin, asprosin). It can be assumed that in patients with MHO, the replenishment of serum NGF occurs under the influence of muscle and adipose tissue. In the MUHO patients only the effect of adipose tissue on NGF was observed. Conclusion. Obesity, regardless of metabolic type, is associated with a decrease in serum NGF concentration. We showed that muscle and adipose tissues make a significant contribution to the serum NGF pool in the MHO patients. In MUHO there is no effect of muscle on the NGF level, but the effect of adipose tissue remains. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=neurotrophins" title="neurotrophins">neurotrophins</a>, <a href="https://publications.waset.org/abstracts/search?q=nerve%20growth%20factor" title=" nerve growth factor"> nerve growth factor</a>, <a href="https://publications.waset.org/abstracts/search?q=NGF" title=" NGF"> NGF</a>, <a href="https://publications.waset.org/abstracts/search?q=brain-derived%20neurotrophic%20factor" title=" brain-derived neurotrophic factor"> brain-derived neurotrophic factor</a>, <a href="https://publications.waset.org/abstracts/search?q=BDNF" title=" BDNF"> BDNF</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolically%20healthy%20obesity" title=" metabolically healthy obesity"> metabolically healthy obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolically%20unhealthy%20obesity" title=" metabolically unhealthy obesity"> metabolically unhealthy obesity</a> </p> <a href="https://publications.waset.org/abstracts/145328/serum-neurotrophins-in-different-metabolic-types-of-obesity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/145328.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">100</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4386</span> Management and Evaluating Technologies of Tissue Engineering Various Fields of Bone</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arash%20Sepehri%20Bonab">Arash Sepehri Bonab</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Techniques to switch cells between development and differentiation, which tend to be commonly exclusive, are utilized in arrange to supply an expansive cell mass that can perform particular separated capacities required for the tissue to develop. Approaches to tissue engineering center on the have to give signals to cell populaces to advance cell multiplication and separation. Current tissue regenerative procedures depend primarily on tissue repair by transplantation of synthetic/natural inserts. In any case, restrictions on the existing procedures have expanded the request for tissue designing approaches. Tissue engineering innovation and stem cell investigation based on tissue building have made awesome advances in overcoming the issues of tissue and organ damage, useful loss, and surgical complications. Bone tissue has the capability to recover itself; in any case, surrenders of a basic estimate anticipate the bone from recovering and require extra support. The advancement of bone tissue building has been utilized to form useful options to recover the bone. This paper primarily portrays current advances in tissue engineering in different fields of bone and talks about the long-term trend of tissue designing innovation in the treatment of complex diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=tissue%20engineering" title="tissue engineering">tissue engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=bone" title=" bone"> bone</a>, <a href="https://publications.waset.org/abstracts/search?q=technologies" title=" technologies"> technologies</a>, <a href="https://publications.waset.org/abstracts/search?q=treatment" title=" treatment"> treatment</a> </p> <a href="https://publications.waset.org/abstracts/149864/management-and-evaluating-technologies-of-tissue-engineering-various-fields-of-bone" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/149864.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">95</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4385</span> Hydroxyapatite Based Porous Scaffold for Tooth Tissue Engineering</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pakize%20Neslihan%20Tasl%C4%B1">Pakize Neslihan Taslı</a>, <a href="https://publications.waset.org/abstracts/search?q=Alev%20Cumbul"> Alev Cumbul</a>, <a href="https://publications.waset.org/abstracts/search?q=Gul%20Merve%20Yalc%C4%B1n"> Gul Merve Yalcın</a>, <a href="https://publications.waset.org/abstracts/search?q=Fikrettin%20Sahin"> Fikrettin Sahin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A key experimental trial in the regeneration of large oral and craniofacial defects is the neogenesis of osseous and ligamentous interfacial structures. Currently, oral regenerative medicine strategies are unpredictable for repair of tooth supporting tissues destroyed as a consequence of trauma, chronic infection or surgical resection. A different approach combining the gel-casting method with Hydroxy Apatite HA-based scaffold and different cell lineages as a hybrid system leads to successively mimic the early stage of tooth development, in vitro. HA is widely accepted as a bioactive material for guided bone and tooth regeneration. In this study, it was reported that, HA porous scaffold preparation, characterization and evaluation of structural and chemical properties. HA is the main factor that exists in tooth and it is in harmony with structural, biological, and mechanical characteristics. Here, this study shows mimicking immature tooth at the late bell stage design and construction of HA scaffolds for cell transplantation of human Adipose Stem Cells (hASCs), human Bone Marrow Stem Cells (hBMSCs) and Gingival Epitelial cells for the formation of human tooth dentin-pulp-enamel complexes in vitro. Scaffold characterization was demonstrated by SEM, FTIR and pore size and density measurements. The biological contraction of dental tissues against each other was demonstrated by mRNA gene expressions, histopatologic observations and protein release profile by ELISA tecnique. The tooth shaped constructs with a pore size ranging from 150 to 300 µm arranged by gathering right amounts of materials provide interconnected macro-porous structure. The newly formed tissue like structures that grow and integrate within the HA designed constructs forming tooth cementum like tissue, pulp and bone structures. These findings are important as they emphasize the potential biological effect of the hybrid scaffold system. In conclusion, this in vitro study clearly demonstrates that designed 3D scaffolds shaped as a immature tooth at the late bell stage were essential to form enamel-dentin-pulp interfaces with an appropriate cell and biodegradable material combination. The biomimetic architecture achieved here is providing a promising platform for dental tissue engineering. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=tooth%20regeneration" title="tooth regeneration">tooth regeneration</a>, <a href="https://publications.waset.org/abstracts/search?q=tissue%20engineering" title=" tissue engineering"> tissue engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=adipose%20stem%20cells" title=" adipose stem cells"> adipose stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=hydroxyapatite%20tooth%20engineering" title=" hydroxyapatite tooth engineering"> hydroxyapatite tooth engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=porous%20scaffold" title=" porous scaffold"> porous scaffold</a> </p> <a href="https://publications.waset.org/abstracts/54703/hydroxyapatite-based-porous-scaffold-for-tooth-tissue-engineering" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54703.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">234</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4384</span> Mediterranean Diet-Driven Changes in Gut Microbiota Decrease the Infiltration of Inflammatory Myeloid Cells into the Intestinal Tissue</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gema%20Go%CC%81mez-Casado">Gema Gómez-Casado</a>, <a href="https://publications.waset.org/abstracts/search?q=Alba%20Rodri%CC%81guez-Mun%CC%83oz"> Alba Rodríguez-Muñoz</a>, <a href="https://publications.waset.org/abstracts/search?q=Virginia%20Mela-Rivas"> Virginia Mela-Rivas</a>, <a href="https://publications.waset.org/abstracts/search?q=Pallavi%20Kompella"> Pallavi Kompella</a>, <a href="https://publications.waset.org/abstracts/search?q=Francisco%20Jose%CC%81%20Tinahones-Maduen%CC%83a"> Francisco José Tinahones-Madueña</a>, <a href="https://publications.waset.org/abstracts/search?q=Isabel%20Moreno-Indias"> Isabel Moreno-Indias</a>, <a href="https://publications.waset.org/abstracts/search?q=Almudena%20Ortega-Go%CC%81mez"> Almudena Ortega-Gómez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Obesity is a high-priority health problem worldwide due to its high prevalence. The proportion of obese and overweight subjects in industrialized countries exceeds half of the population in most cases. Beyond the metabolic problem, obesity boosts inflammation levels in the organism. The gut microbiota, considered an organ by itself, controls a high variety of processes at a systemic level. In fact, the microbiota interacts closely with the immune system, being crucial in determining the maturation state of neutrophils, key effectors of the innate immune response. It is known that changes in the diet exert strong effects on the variety and activity of the gut microbiota. The effect that those changes have on the axis microbiota-immune response is an unexplored field. In this study, 10 patients with obesity (weight 114,3 ± 14,5Kg, BMI 40,47±3,66) followed a Mediterranean-hypocaloric diet for 3 months, reducing their initial weight by 12,71 ± 3%. A transplant of microbiota from these patients before and after the diet was performed into wild type “germ-free” mice (n=10/group), treated with antibiotics. Six weeks after the transplant, mice were euthanized, and the presence of cells from the innate immune system were analysed in different organs (bone marrow, blood, spleen, visceral adipose tissue, and intestine) by flow cytometry. No differences were observed in the number of myeloid cells in bone marrow, blood, spleen, or visceral adipose tissue of mice transplanted with patient’s microbiota before and after following the Mediterranean diet. However, the intestine of mice that received post-diet microbiota presented a marked decrease in the number of neutrophils (whose presence is associated with tissue inflammation), as well as macrophages. In line with these findings, intestine monocytes from mice with post-diet microbiota showed a less inflammatory profile (lower Ly6Gˡᵒʷ proportion of cells). These results point toward a decrease in the inflammatory state of the intestinal tissue, derived from changes in the gut microbiota, which occurred after a 3-month Mediterranean diet. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=obesity" title="obesity">obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=nutrition" title=" nutrition"> nutrition</a>, <a href="https://publications.waset.org/abstracts/search?q=Mediterranean%20diet" title=" Mediterranean diet"> Mediterranean diet</a>, <a href="https://publications.waset.org/abstracts/search?q=gut%20microbiota" title=" gut microbiota"> gut microbiota</a>, <a href="https://publications.waset.org/abstracts/search?q=immune%20system" title=" immune system"> immune system</a> </p> <a href="https://publications.waset.org/abstracts/157501/mediterranean-diet-driven-changes-in-gut-microbiota-decrease-the-infiltration-of-inflammatory-myeloid-cells-into-the-intestinal-tissue" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/157501.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">127</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4383</span> Relationship between Conjugated Linoleic Acid Intake, Biochemical Parameters and Body Fat among Adults and Elderly</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marcela%20Menah%20de%20Sousa%20Lima">Marcela Menah de Sousa Lima</a>, <a href="https://publications.waset.org/abstracts/search?q=Victor%20Ushijima%20Leone"> Victor Ushijima Leone</a>, <a href="https://publications.waset.org/abstracts/search?q=Natasha%20Aparecida%20Grande%20de%20Franca"> Natasha Aparecida Grande de Franca</a>, <a href="https://publications.waset.org/abstracts/search?q=Barbara%20Santarosa%20Emo%20Peters"> Barbara Santarosa Emo Peters</a>, <a href="https://publications.waset.org/abstracts/search?q=Ligia%20Araujo%20Martini"> Ligia Araujo Martini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Conjugated linoleic acid (CLA) intake has been constantly related to benefits to human health since having a positive effect on reducing body fat. The aim of the present study was to investigate the association between CLA intake and biochemical measurements and body composition of adults and the elderly. Subjects/Methods: 287 adults and elderly participants in an epidemiological study in Sao Paulo Brazil, were included in the present study. Participants had their dietary data obtained by two non-consecutive 24HR, a body composition assessed by dual-energy absorptiometry exam (DXA), and a blood collection. Mean differences and a correlation test was performed. For all statistical tests, a significance of 5% was considered. Results: CLA intake showed a positive correlation with HDL-c levels (r = 0.149; p = 0.011) and negative with VLDL-c levels (r = -0.134; p = 0.023), triglycerides (r = -0.135; p = 0.023) and glycemia (r = -0.171; p = 0.004), as well as negative correlation with visceral adipose tissue (VAT) (r = -0.124, p = 0.036). Evaluating individuals in two groups according to VAT values, a significant difference in CLA intake was observed (p = 0.041), being the group with the highest VAT values, the one with the lowest fatty acid intake. Conclusions: This study suggests that CLA intake is associated with a better lipid profile and lower visceral adipose tissue volume, which contributes to the investigation of the effects of CLA on obesity parameters. However, it is necessary to investigate the effects of CLA from milk and dairy products in the control adiposity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adiposity" title="adiposity">adiposity</a>, <a href="https://publications.waset.org/abstracts/search?q=dairy%20products" title=" dairy products"> dairy products</a>, <a href="https://publications.waset.org/abstracts/search?q=diet" title=" diet"> diet</a>, <a href="https://publications.waset.org/abstracts/search?q=fatty%20acids" title=" fatty acids"> fatty acids</a> </p> <a href="https://publications.waset.org/abstracts/116449/relationship-between-conjugated-linoleic-acid-intake-biochemical-parameters-and-body-fat-among-adults-and-elderly" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116449.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4382</span> Fatty Acid Translocase (Cd36), Energy Substrate Utilization, and Insulin Signaling in Brown Adipose Tissue in Spontaneously Hypertensive Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Michal%20Pravenec">Michal Pravenec</a>, <a href="https://publications.waset.org/abstracts/search?q=Miroslava%20Simakova"> Miroslava Simakova</a>, <a href="https://publications.waset.org/abstracts/search?q=Jan%20Silhavy"> Jan Silhavy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Brown adipose tissue (BAT) plays an important role in lipid and glucose metabolism in rodents and possibly also in humans. Recently, using systems genetics approach in the BAT from BXH/HXB recombinant inbred strains, derived from the SHR (spontaneously hypertensive rat) and BN (Brown Norway) progenitors, we identified Cd36 (fatty acid translocase) as the hub gene of co-expression module associated with BAT relative weight and function. An important aspect of BAT biology is to better understand the mechanisms regulating the uptake and utilization of fatty acids and glucose. Accordingly, BAT function in the SHR that harbors mutant nonfunctional Cd36 variant (hereafter referred to as SHR-Cd36⁻/⁻) was compared with SHR transgenic line expressing wild type Cd36 under control of a universal promoter (hereafter referred to as SHR-Cd36⁺/⁺). BAT was incubated in media containing insulin and 14C-U-glucose alone or 14C-U-glucose together with palmitate. Incorporation of glucose into BAT lipids was significantly higher in SHR-Cd36⁺/⁺ versus SHR-Cd36⁻/⁻ rats when incubation media contained glucose alone (SHR-Cd36⁻/⁻ 591 ± 75 vs. SHR-Cd36⁺/⁺ 1036 ± 135 nmol/gl./2h; P < 0.005). Adding palmitate into incubation media had no effect in SHR-Cd36⁻/⁻ rats but significantly reduced glucose incorporation into BAT lipids in SHR-Cd36⁺/⁺ (SHR-Cd36⁻/⁻ 543 ± 55 vs. SHR-Cd36⁺/⁺ 766 ± 75 nmol/gl./2h; P < 0.05 denotes significant Cd36 x palmitate interaction determined by two-way ANOVA). This Cd36-dependent reduced glucose uptake in SHR-Cd36⁺/⁺ BAT was likely secondary to increased palmitate incorporation and utilization due to the presence of wild type Cd36 fatty acid translocase in transgenic rats. This possibility is supported by increased incorporation of 14C-U-palmitate into BAT lipids in the presence of both palmitate and glucose in incubation media (palmitate alone: SHR-Cd36⁻/⁻ 870 ± 21 vs. SHR-Cd36⁺/⁺ 899 ± 42; glucose+palmitate: SHR-Cd36⁻/⁻ 899 ± 47 vs. SHR-Cd36⁺/⁺ 1460 ± 111 nmol/palm./2h; P < 0.05 denotes significant Cd36 x glucose interaction determined by two-way ANOVA). It is possible that addition of glucose into the incubation media increased palmitate incorporation into BAT lipids in SHR-Cd36⁺/⁺ rats because of glucose availability for glycerol phosphate production and increased triglyceride synthesis. These changes in glucose and palmitate incorporation into BAT lipids were associated with significant differential expression of Irs1, Irs2, Slc2a4 and Foxo1 genes involved in insulin signaling and glucose metabolism only in SHR-Cd36⁺/⁺ rats which suggests Cd36-dependent effects on insulin action. In conclusion, these results provide compelling evidence that Cd36 plays an important role in BAT insulin signaling and energy substrate utilization. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=brown%20adipose%20tissue" title="brown adipose tissue">brown adipose tissue</a>, <a href="https://publications.waset.org/abstracts/search?q=Cd36" title=" Cd36"> Cd36</a>, <a href="https://publications.waset.org/abstracts/search?q=energy%20substrate%20utilization" title=" energy substrate utilization"> energy substrate utilization</a>, <a href="https://publications.waset.org/abstracts/search?q=insulin%20signaling" title=" insulin signaling"> insulin signaling</a>, <a href="https://publications.waset.org/abstracts/search?q=spontaneously%20hypertensive%20rat" title=" spontaneously hypertensive rat"> spontaneously hypertensive rat</a> </p> <a href="https://publications.waset.org/abstracts/114284/fatty-acid-translocase-cd36-energy-substrate-utilization-and-insulin-signaling-in-brown-adipose-tissue-in-spontaneously-hypertensive-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/114284.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4381</span> The Ability of Adipose Derived Mesenchymal Stem Cells for Diabetes Mellitus Type 2 Treatment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Purwati">Purwati</a>, <a href="https://publications.waset.org/abstracts/search?q=Sony%20Wibisono"> Sony Wibisono</a>, <a href="https://publications.waset.org/abstracts/search?q=Ari%20Sutjahjo"> Ari Sutjahjo</a>, <a href="https://publications.waset.org/abstracts/search?q=Askandar%20T.%20J."> Askandar T. J.</a>, <a href="https://publications.waset.org/abstracts/search?q=Fedik%20A.%20Rantam"> Fedik A. Rantam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diabetes mellitus type 2 (T2DM), also known as hyperglycemia, results from insulin resistance and relative insulin deficiency. Diabetes mellitus is the main cause of premature death, particularly among individuals under the age of 70 years old. Mesenchymal stem cells (MSCs) can release bioactive molecules that promote tissue repair and regeneration. Hence, in this research, we evaluated the potential of autologous adipose-derived mesenchymal stem cells (AD-MSCs) in 40 patients of phase I clinical trial in T2DM with various ages between 30-79 years. AD-MSCs are transferred through catheterization. MSCs were validated by measures of CD105+ and CD34- expression. The result showed that after AD-MSCs transplantation, blood glucose levels (fasting and 2-hour postprandial) and insulin levels were significantly decreasing. Besides that, the level of HbA1c significantly decreased after three months of AD-MSCs injection and increasing level of c-peptide after injection. Thus, we conclude that AD-MSCs injection has the potential for T2DM therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=glucose" title="glucose">glucose</a>, <a href="https://publications.waset.org/abstracts/search?q=hyperglycemia" title=" hyperglycemia"> hyperglycemia</a>, <a href="https://publications.waset.org/abstracts/search?q=MSCs" title=" MSCs"> MSCs</a>, <a href="https://publications.waset.org/abstracts/search?q=T2DM" title=" T2DM"> T2DM</a> </p> <a href="https://publications.waset.org/abstracts/171671/the-ability-of-adipose-derived-mesenchymal-stem-cells-for-diabetes-mellitus-type-2-treatment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171671.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">81</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4380</span> Design and Fabrication of a Scaffold with Appropriate Features for Cartilage Tissue Engineering</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20S.%20Salehi">S. S. Salehi</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Shamloo"> A. Shamloo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Poor ability of cartilage tissue when experiencing a damage leads scientists to use tissue engineering as a reliable and effective method for regenerating or replacing damaged tissues. An artificial tissue should have some features such as biocompatibility, biodegradation and, enough mechanical properties like the original tissue. In this work, a composite hydrogel is prepared by using natural and synthetic materials that has high porosity. Mechanical properties of different combinations of polymers such as modulus of elasticity were tested, and a hydrogel with good mechanical properties was selected. Bone marrow derived mesenchymal stem cells were also seeded into the pores of the sponge, and the results showed the adhesion and proliferation of cells within the hydrogel after one month. In comparison with previous works, this study offers a new and efficient procedure for the fabrication of cartilage like tissue and further cartilage repair. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cartilage%20tissue%20engineering" title="cartilage tissue engineering">cartilage tissue engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrogel" title=" hydrogel"> hydrogel</a>, <a href="https://publications.waset.org/abstracts/search?q=mechanical%20strength" title=" mechanical strength"> mechanical strength</a>, <a href="https://publications.waset.org/abstracts/search?q=mesenchymal%20stem%20cell" title=" mesenchymal stem cell"> mesenchymal stem cell</a> </p> <a href="https://publications.waset.org/abstracts/65407/design-and-fabrication-of-a-scaffold-with-appropriate-features-for-cartilage-tissue-engineering" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/65407.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">300</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4379</span> Protein Feeding Pattern, Casein Feeding, or Milk-Soluble Protein Feeding did not Change the Evolution of Body Composition during a Short-Term Weight Loss Program</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Solange%20Adechian">Solange Adechian</a>, <a href="https://publications.waset.org/abstracts/search?q=Mich%C3%A8le%20Balage"> Michèle Balage</a>, <a href="https://publications.waset.org/abstracts/search?q=Didier%20Remond"> Didier Remond</a>, <a href="https://publications.waset.org/abstracts/search?q=Carole%20Mign%C3%A9"> Carole Migné</a>, <a href="https://publications.waset.org/abstracts/search?q=Annie%20Quignard-Boulang%C3%A9"> Annie Quignard-Boulangé</a>, <a href="https://publications.waset.org/abstracts/search?q=Agn%C3%A8s%20Marset-Baglieri"> Agnès Marset-Baglieri</a>, <a href="https://publications.waset.org/abstracts/search?q=Sylvie%20Rousset"> Sylvie Rousset</a>, <a href="https://publications.waset.org/abstracts/search?q=Yves%20Boirie"> Yves Boirie</a>, <a href="https://publications.waset.org/abstracts/search?q=Claire%20Gaudichon"> Claire Gaudichon</a>, <a href="https://publications.waset.org/abstracts/search?q=Dominique%20Dardevet"> Dominique Dardevet</a>, <a href="https://publications.waset.org/abstracts/search?q=Laurent%20Mosoni"> Laurent Mosoni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Studies have shown that timing of protein intake, leucine content, and speed of digestion significantly affect postprandial protein utilization. Our aim was to determine if one can spare lean body mass during energy restriction by varying the quality and the timing of protein intake. Obese volunteers followed a 6-wk restricted energy diet. Four groups were compared: casein pulse, casein spread, milk-soluble protein (MSP, = whey) pulse, and MSP spread (n = 10-11 per group). In casein groups, caseins were the only protein source; it was MSP in MSP groups. Proteins were distributed in four meals per day in the proportion 8:80:4:8% in the pulse groups; it was 25:25:25:25% in the spread groups. We measured weight, body composition, nitrogen balance, 3-methylhistidine excretion, perception of hunger, plasma parameters, adipose tissue metabolism, and whole body protein metabolism. Volunteers lost 7.5 ± 0.4 kg of weight, 5.1 ± 0.2 kg of fat, and 2.2 ± 0.2 kg of lean mass, with no difference between groups. In adipose tissue, cell size and mRNA expression of various genes were reduced with no difference between groups. Hunger perception was also never different between groups. In the last week, due to a higher inhibition of protein degradation and despite a lower stimulation of protein synthesis, postprandial balance between whole body protein synthesis and degradation was better with caseins than with MSP. It seems likely that the positive effect of caseins on protein balance occurred only at the end of the experiment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lean%20body%20mass" title="lean body mass">lean body mass</a>, <a href="https://publications.waset.org/abstracts/search?q=fat%20mass" title=" fat mass"> fat mass</a>, <a href="https://publications.waset.org/abstracts/search?q=casein" title=" casein"> casein</a>, <a href="https://publications.waset.org/abstracts/search?q=whey" title=" whey"> whey</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20metabolism" title=" protein metabolism"> protein metabolism</a> </p> <a href="https://publications.waset.org/abstracts/175765/protein-feeding-pattern-casein-feeding-or-milk-soluble-protein-feeding-did-not-change-the-evolution-of-body-composition-during-a-short-term-weight-loss-program" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175765.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">72</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=adipose%20tissue%20engineering&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=adipose%20tissue%20engineering&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=adipose%20tissue%20engineering&page=4">4</a></li> <li class="page-item"><a class="page-link" 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