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Dipòsit Digital de la Universitat de Barcelona: The immunohistochemical expression of programmed death ligand 1 (PD-L1) is affected by sample overfixation
<!DOCTYPE html> <html> <head> <title>Dipòsit Digital de la Universitat de Barcelona: The immunohistochemical expression of programmed death ligand 1 (PD-L1) is affected by sample overfixation</title> <base href="https://diposit.ub.edu" /> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> <meta name="Generator" content="DSpace 5.8" /> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <link rel="shortcut icon" href="/dspace/favicon.ico" type="image/x-icon"/> <link rel="stylesheet" href="/dspace/static/css/jquery-ui-1.10.3.custom/redmond/jquery-ui-1.10.3.custom.css" type="text/css" /> <link rel="stylesheet" href="/dspace/static/css/bootstrap/bootstrap.min.css" type="text/css" /> <link rel="stylesheet" href="/dspace/static/css/bootstrap/bootstrap-theme.min.css" type="text/css" /> <link rel="stylesheet" href="/dspace/static/css/bootstrap/dspace-theme.css" type="text/css" /> <link rel="search" type="application/opensearchdescription+xml" href="/dspace/open-search/description.xml" title="DSpace"/> <link rel="schema.DCTERMS" href="http://purl.org/dc/terms/" /> <link rel="schema.DC" href="http://purl.org/dc/elements/1.1/" /> <meta name="DC.creator" content="Barber脿, Angels" /> <meta name="DC.creator" content="Marginet-Flinch, Ruth" /> <meta name="DC.creator" content="Martin, Montserrat" /> <meta name="DC.creator" content="Mate, Jos茅 L." /> <meta name="DC.creator" content="Oriol, Albert" /> <meta name="DC.creator" content="Mart铆nez Soler, Fina" /> <meta name="DC.creator" content="Santaluc铆a Albi, Tom脿s" /> <meta name="DC.creator" content="Fern谩ndez, Pedro L." /> <meta name="DCTERMS.dateAccepted" content="2024-09-16T14:52:06Z" scheme="DCTERMS.W3CDTF" /> <meta name="DCTERMS.available" content="2024-09-16T14:52:06Z" scheme="DCTERMS.W3CDTF" /> <meta name="DCTERMS.issued" content="2020-03-03" scheme="DCTERMS.W3CDTF" /> <meta name="DC.identifier" content="1541-2016" /> <meta name="DC.identifier" content="https://hdl.handle.net/2445/215182" scheme="DCTERMS.URI" /> <meta name="DCTERMS.abstract" content="Humanized antibodies targeting programmed death receptor 1 (PD-1) or its ligand (PD-L1) have been approved for the treatment of different cancers. Some of these antibodies show a correlation between the tissue expression of PD-L1 and response. Evaluation of PD-L1 expression presents multiple challenges, but some preanalytical issues such as tissue fixation have been scarcely evaluated. With the hypothesis that immunohistochemical staining of PD-L1 may be impacted by the time of specimen fixation, we evaluated differences in its expression in tonsil samples exposed to predefined fixation times. Random nontumoral tonsillectomy specimens were blindly evaluated in tissue microarray slides after staining with SP142 and SP263 antibodies. With fixation times ranging from 12 to 72 hours, between 2.8% and 6.1% of the samples were considered to be suboptimally stained, with no differences between the 2 antibodies within these fixation times. A significantly higher proportion of samples exposed to a fixation time of 96 hours presented suboptimal immunostaining (15.6%, P<0.0001). In addition, suboptimally stained spots were 20.8% using SP142 and 10.4% using SP263 after 96 hours of fixation (P=0.046). In conclusion, the quality of staining for PD-L1 in tonsil samples decreased with overfixation of the specimen at times >72 hours. Samples exposed to formaldehyde for longer periods presented suboptimal results for both clones, but the SP142 antibody presented a significantly lower tolerance to formalin overexposure than SP263. These results indicate the relevance of a controlled preanalytical processing of samples and particularly the length of fixation of tumor specimens." /> <meta name="DCTERMS.extent" content="6 p." /> <meta name="DC.format" content="application/pdf" /> <meta name="DC.language" content="eng" scheme="DCTERMS.RFC1766" /> <meta name="DC.publisher" content="Wolters Kluwer Health" /> <meta name="DCTERMS.isFormatOf" content="Versi贸 postprint del document publicat a: https://doi.org/10.1097/PAI.0000000000000847" /> <meta name="DCTERMS.isPartOf" content="Applied Immunohistochemistry & Molecular Morphology, 2020, vol. 29, num.1, p. 76-81" /> <meta name="DC.relation" content="https://doi.org/10.1097/PAI.0000000000000847" scheme="DCTERMS.URI" /> <meta name="DC.rights" content="(c) Wolters Kluwer Health, 2020" /> <meta name="DC.source" content="Articles publicats en revistes (Infermeria Fonamental i Cl铆nica)" /> <meta name="DC.subject" content="Anticossos monoclonals" /> <meta name="DC.subject" content="Regulaci贸 gen猫tica" /> <meta name="DC.subject" content="Bios铆ntesi" /> <meta name="DC.subject" content="Monoclonal antibodies" /> <meta name="DC.subject" content="Genetic regulation" /> <meta name="DC.subject" content="Biosynthesis" /> <meta name="DC.title" content="The immunohistochemical expression of programmed death ligand 1 (PD-L1) is affected by sample overfixation" /> <meta name="DC.type" content="info:eu-repo/semantics/article" /> <meta name="DC.type" content="info:eu-repo/semantics/acceptedVersion" /> <meta name="DC.identifier" content="700429" /> <meta name="DC.date" content="2024-09-16T14:52:06Z" scheme="DCTERMS.W3CDTF" /> <meta name="DC.rights" content="info:eu-repo/semantics/openAccess" /> <meta name="DC.identifier" content="32134754" /> <meta name="citation_keywords" content="info:eu-repo/semantics/article; info:eu-repo/semantics/acceptedVersion" /> <meta name="citation_title" content="The immunohistochemical expression of programmed death ligand 1 (PD-L1) is affected by sample overfixation" /> <meta name="citation_issn" content="1541-2016" /> <meta name="citation_publisher" content="Wolters Kluwer Health" /> <meta name="citation_language" content="eng" /> <meta name="citation_author" content="Barber脿, Angels" /> <meta name="citation_author" content="Marginet-Flinch, Ruth" /> <meta name="citation_author" content="Martin, Montserrat" /> <meta name="citation_author" content="Mate, Jos茅 L." /> <meta name="citation_author" content="Oriol, Albert" /> <meta name="citation_author" content="Mart铆nez Soler, Fina" /> <meta name="citation_author" content="Santaluc铆a Albi, Tom脿s" /> <meta name="citation_author" content="Fern谩ndez, Pedro L." /> <meta name="citation_pdf_url" content="https://diposit.ub.edu/dspace/bitstream/2445/215182/1/228242.pdf" /> <meta name="citation_date" content="2020-03-03" /> <meta name="citation_abstract_html_url" content="https://diposit.ub.edu/dspace/handle/2445/215182" /> <script type='text/javascript' src="/dspace/static/js/jquery/jquery-1.10.2.min.js"></script> <script type='text/javascript' src='/dspace/static/js/jquery/jquery-ui-1.10.3.custom.min.js'></script> <script type='text/javascript' src='/dspace/static/js/bootstrap/bootstrap.min.js'></script> <script type='text/javascript' src='/dspace/static/js/holder.js'></script> <script type="text/javascript" src="/dspace/utils.js"></script> <script type="text/javascript" src="/dspace/static/js/choice-support.js"> </script> <!-- /* <script type="text/javascript"> var _gaq = _gaq || []; _gaq.push(['_setAccount', 'UA-9816681-2']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 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Some of these antibodies show a correlation between the tissue expression of PD-L1 and response. Evaluation of PD-L1 expression presents multiple challenges, but some preanalytical issues such as tissue fixation have been scarcely evaluated. With the hypothesis that immunohistochemical staining of PD-L1 may be impacted by the time of specimen fixation, we evaluated differences in its expression in tonsil samples exposed to predefined fixation times. Random nontumoral tonsillectomy specimens were blindly evaluated in tissue microarray slides after staining with SP142 and SP263 antibodies. With fixation times ranging from 12 to 72 hours, between 2.8% and 6.1% of the samples were considered to be suboptimally stained, with no differences between the 2 antibodies within these fixation times. A significantly higher proportion of samples exposed to a fixation time of 96 hours presented suboptimal immunostaining (15.6%, P<0.0001). In addition, suboptimally stained spots were 20.8% using SP142 and 10.4% using SP263 after 96 hours of fixation (P=0.046). In conclusion, the quality of staining for PD-L1 in tonsil samples decreased with overfixation of the specimen at times >72 hours. Samples exposed to formaldehyde for longer periods presented suboptimal results for both clones, but the SP142 antibody presented a significantly lower tolerance to formalin overexposure than SP263. These results indicate the relevance of a controlled preanalytical processing of samples and particularly the length of fixation of tumor specimens.</td></tr> <tr><td class="metadataFieldLabel">Note: </td><td class="metadataFieldValue">Versi贸 postprint del document publicat a: https://doi.org/10.1097/PAI.0000000000000847</td></tr> <tr><td class="metadataFieldLabel">It is part of: </td><td class="metadataFieldValue">Applied Immunohistochemistry & Molecular Morphology, 2020, vol. 29, num.1, p. 76-81</td></tr> <tr><td class="metadataFieldLabel">URI: </td><td class="metadataFieldValue"><a href="https://hdl.handle.net/2445/215182">https://hdl.handle.net/2445/215182</a></td></tr> <tr><td class="metadataFieldLabel">Related resource: </td><td class="metadataFieldValue"><a href="https://doi.org/10.1097/PAI.0000000000000847">https://doi.org/10.1097/PAI.0000000000000847</a></td></tr> <tr><td class="metadataFieldLabel">ISSN: </td><td class="metadataFieldValue">1541-2016</td></tr> <tr><td class="metadataFieldLabel">Appears in Collections:</td><td class="metadataFieldValue"><a href="/dspace/handle/2445/18332">Articles publicats en revistes (Infermeria Fonamental i Cl铆nica)</a><br/></td></tr> </table><br/> <div class="panel panel-info"><div class="panel-heading">Files in This Item:</div> <table class="table panel-body"><tr><th id="t1" class="standard">File</th> <th id="t2" class="standard">Description</th> <th id="t3" class="standard">Size</th><th id="t4" class="standard">Format</th><th> </th></tr> <tr><td headers="t1" class="standard"><a target="_blank" href="/dspace/bitstream/2445/215182/1/228242.pdf">228242.pdf</a></td><td headers="t2" class="standard"></td><td headers="t3" class="standard">257.14 kB</td><td headers="t4" class="standard">Adobe PDF</td><td class="standard" align="center"><a class="btn btn-primary" target="_blank" href="/dspace/bitstream/2445/215182/1/228242.pdf">View/Open</a></td></tr></table> </div> <div class="container row"> <a class="btn btn-default" href="/dspace/handle/2445/215182?mode=full"> Show full item record </a> </div> <br/> <br/> <p class="submitFormHelp alert alert-info"> Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated. <!-- a href=""><img src="/dspace/image/cc-somerights.gif" border="0" alt="Creative Commons" style="margin-top: -5px;" class="pull-right"/></a --> <!-- %= cc_rdf % --> </p> <div class="addthis_sharing_toolbox"></div> </div> </main> <footer class="navbar navbar-inverse navbar-bottom" style='background:-webkit-linear-gradient(#6685a9,#406086);background:-o-linear-gradient(#6685a9,#406086);background:-moz-linear-gradient(#6685a9,#406086);background:linear-gradient(#6685a9,#406086);border-radius:0;border:0;width:100%'> <div id="designedby" class="container text-muted"> <!-- fmt:message key="jsp.layout.footer-default.theme-by"/> <a href="http://www.cineca.it"><img src="/dspace/image/logo-cineca-small.png" alt="Logo CINECA" /></a --> <div id="footer_feedback" class="pull-left"> <p class="text-muted"> <span style='color:white'>Coordination: </span> <a style='color:#000' onMouseOver="this.style.color='#fff'" onMouseOut="this.style.color='#000'" target='_blank' href='http://crai.ub.edu/en'> CRAI Universitat de Barcelona</a> <a style='color:#000' onMouseOver="this.style.color='#fff'" onMouseOut="this.style.color='#000'" target="_blank" href='http://www.ub.edu/web/ub/en/menu_peu/avislegal/avis_legal.html'>Legal notice</a> </p> </div> <div id="footer_feedback" class="pull-right"> <p class="text-muted"> <span style='color:white'>Metadata subject to: </span> <a target='_blank' href='http://creativecommons.org/publicdomain/zero/1.0/'> <img src="/dspace/image/logoCC.png" alt="Logo CC" /> </a> <span style='color:white'>Powered by: </span> <a style='color:#000' onMouseOver="this.style.color='#fff'" onMouseOut="this.style.color='#000'" target="_blank" href="http://dspace.org/">DSpace</a> <a style='color:#000' onMouseOver="this.style.color='#fff'" onMouseOut="this.style.color='#000'" target="_blank" href="/dspace/feedback">Contact us</a> </p> </div> <!-- div id="footer_feedback" class="pull-right"> <p class="text-muted"><a target="_blank" href="http://www.dspace.org/">DSpace Software</a> Copyright © 2002-2013 <a target="_blank" href="http://www.duraspace.org/">Duraspace</a> - <a target="_blank" href="/dspace/feedback">Contact us</a> <a href="/dspace/htmlmap"></a></p> </div --> </div> </footer> </body> </html>