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GhostKOALA
<!DOCTYPE html> <html lang="en"> <head> <meta charset="utf-8"> <title>GhostKOALA</title> <link rel="stylesheet" href="/css/kegg25.css"> <script> <!-- function submit_check(form) { //input_file var ent = form.input_file.value; ent = ent.replace( /\s+$/, "" ); ent = ent.replace( /^\s+/, "" ); var ent2 = form.sequence_data.value; ent2 = ent2.replace( /\s+$/, "" ); ent2 = ent2.replace( /^\s+/, "" ); if(ent == "" && ent2 == "") { alert('sequence data is empty'); return false; } //email ent = form.email.value; ent = ent.replace( /\s+$/, "" ); ent = ent.replace( /^\s+/, "" ); if(ent == "") { alert('email is empty'); return false; } form.action='/tool-bin/blastkoala_request'; form.submit(); return true; } --> </script> <style> td.td10 {text-align:center; padding:4px;} </style> </head> <body> <div id="header"> <div class="logo"> <a href="/kegg/"><img src="/Fig/kegg128.gif" alt="KEGG icon"></a> </div> <div class="title"> <h2 class="txt1">GhostKOALA</h2> <h5>Automatic KO assignment and KEGG mapping service</h5> </div> </div> <div style="width:704px; margin:4px 20px; border:2px solid #f0e0e4;"> <table style="width:700px; border-collapse:collapse; background-color:#f0e0e4;"> <tr> <td class="td10"><a href="../blastkoala/"><b>BlastKOALA</b></a></td> <td class="td10"><span class="current"><b>GhostKOALA</b></span></td> <td class="td10"><a href="https://www.genome.jp/tools/kofamkoala/"><b>KofamKOALA</b></a></td> </tr> </table> <div class="main"> <br> <!-- <div class="bor1"> <span style="color:red;">message.</span> </div> <br> --> <div class="frame1" style="text-align:center;"> <b class="title10">KOALA job status</b> 2025/02/18 00:54:43 (GMT+9) <table align="center"> <tr><td></td><td>Blast</td><td>Ghost</td><td>Kofam</td></tr> <tr><td>Number of jobs in the queue</td><td>0</td><td>1</td><td>1</td></tr> <tr><td>Submission of last completed job</td><td>2025/02/18 00:43:55</td><td>2025/02/18 00:48:55</td><td>2025/02/18 00:39:59</td></tr> </table> </div> <br> GhostKOALA assigns K numbers to the user's sequence data by GHOSTX search against reference GENES datasets, the same datasets used by BlastKOALA. See <a href="/blastkoala/help_ghostkoala.html">Step-by-step Instructions</a>. <br><br> <hr class="frame1"> <br> <form enctype="multipart/form-data" method="post" name="show" action="/tool-bin/blastkoala_request"> <b>Upload query amino acid sequences in FASTA format</b><br> <div class="indent2"> Enter FASTA sequences<br> <textarea id="s_q" name="sequence_data" cols="80" rows="5"></textarea> <br> Or upload file: <input type="file" name="input_file" size="34"> </div> <div class="indent"> Your query data consisting of multiple amino acid sequences will be given K numbers by GhostKOALA.<br> The file size of up to 300 MB with the limit of 500,000 sequences may be uploaded. </div> <br> <b>Select reference GENES datasets to be searched</b><br> <div class="indent2"> <input type="radio" name="db_type" value="refeuk+refprok+viruses"> Eukaryotes + Prokaryotes + Viruses<br> <input type="radio" name="db_type" value="refprok+viruses"> Prokaryotes + Viruses </div> <div class="indent"> Eukaryotes and prokaryotes contain sequences of KEGG reference genomes and sequences linked from pubmed records of KO data. Viruses contain all sequences of KEGG viruses. </div> <br> <b>Enter your email address</b> (institutional only, no free emails)<br> <div class="indent2"> <input type="text" name="email" size="60" value=""> <div class="indent2"> <input type="button" onclick="submit_check(this.form);" value="Request for email confirmation"> <input type="reset" value="Clear"> <input type="hidden" name="type" value="ghostkoala"> </div> </div> <div class="indent"> An email will be sent to you for confirmation of your input data. You will have to click on the link in the email to initiate your job. When the job is finished, you will receive another email for browsing the result and performing KEGG Mapper analysis. You cannot request another job until the current one is finished or canceled. <i>Notice: Your email address will not be used for any other purpose.</i> </div> </form> <br> <b>Reference</b> <ol> <li>Kanehisa, M., Sato, Y., and Morishima, K.; BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences. J. Mol. Biol. 428, 726-731 (2016). [<a href="http://www.ncbi.nlm.nih.gov/pubmed/26585406">pubmed</a>] [<a href="https://doi.org/10.1016/j.jmb.2015.11.006">pdf</a>] </li> </ol> <br> <div style="float:left;"> <i>Last updated: December 1, 2024</i> </div> <div style="float:right;"> <a href="javascript:void(window.open('../blastkoala/version_blastkoala.html','BlastKOALA_Version','toolbar=no,location=no,directories=no,width=720,height=640,resizable=yes,scrollbars=yes'))"><i>Version 3.1</i></a> </div> <div style="clear:both;"></div> </div> </div> </body> </html>