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quantification</title> <meta name="description" content="Search results for: quantification"> <meta name="keywords" content="quantification"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="quantification"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 528</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: quantification</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">528</span> Toward Subtle Change Detection and Quantification in Magnetic Resonance Neuroimaging</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Esmaeilpour">Mohammad Esmaeilpour</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One of the important open problems in the field of medical image processing is detection and quantification of small changes. In this poster, we try to investigate that, how the algebraic decomposition techniques can be used for semiautomatically detecting and quantifying subtle changes in Magnetic Resonance (MR) neuroimaging volumes. We mostly focus on the low-rank values of the matrices achieved from decomposing MR image pairs during a period of time. Besides, a skillful neuroradiologist will help the algorithm to distinguish between noises and small changes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=magnetic%20resonance%20neuroimaging" title="magnetic resonance neuroimaging">magnetic resonance neuroimaging</a>, <a href="https://publications.waset.org/abstracts/search?q=subtle%20change%20detection%20and%20quantification" title=" subtle change detection and quantification"> subtle change detection and quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=algebraic%20decomposition" title=" algebraic decomposition"> algebraic decomposition</a>, <a href="https://publications.waset.org/abstracts/search?q=basis%20functions" title=" basis functions"> basis functions</a> </p> <a href="https://publications.waset.org/abstracts/32372/toward-subtle-change-detection-and-quantification-in-magnetic-resonance-neuroimaging" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32372.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">474</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">527</span> Theater Metaphor in Event Quantification: A Corpus Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zhuo%20Jing-Schmidt">Zhuo Jing-Schmidt</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun%20Lang"> Jun Lang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Numeral classifiers are common in Asian languages. Research on numeral classifiers primarily focuses on noun classifiers that quantify and individuate nominal referents. There is a scarcity of research on event quantification using verb classifiers. This study aims to understand the semantic and conceptual basis of event quantification in Chinese. From a usage-based Construction Grammar perspective, this study presents a corpus analysis of event quantification in Chinese. Drawing on a large balanced corpus of contemporary Chinese, we analyze 667 NOUN col-lexemes totaling 31136 tokens of a productive numeral classifier construction in Chinese. Using collostructional analysis of the collexemes, the results show that the construction quantifies and classifies dramatic events using a theater-based conceptual metaphor. We argue that the usage patterns reflect the cultural entrenchment of theater as in Chinese conceptualization and the construal of theatricality in linguistic expression. The study has implications for cognitive semantics and construction grammar. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=event%20quantification" title="event quantification">event quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=classifier" title=" classifier"> classifier</a>, <a href="https://publications.waset.org/abstracts/search?q=corpus" title=" corpus"> corpus</a>, <a href="https://publications.waset.org/abstracts/search?q=metaphor" title=" metaphor"> metaphor</a> </p> <a href="https://publications.waset.org/abstracts/171981/theater-metaphor-in-event-quantification-a-corpus-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171981.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">85</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">526</span> CO2 Emissions Quantification of the Modular Bridge Superstructure</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chanhyuck%20Jeon">Chanhyuck Jeon</a>, <a href="https://publications.waset.org/abstracts/search?q=Jongho%20Park"> Jongho Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Jinwoong%20Choi"> Jinwoong Choi</a>, <a href="https://publications.waset.org/abstracts/search?q=Sungnam%20Hong"> Sungnam Hong</a>, <a href="https://publications.waset.org/abstracts/search?q=Sun-Kyu%20Park"> Sun-Kyu Park</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Many industries put emphasis on environmentally-friendliness as environmental problems are on the rise all over the world. Among themselves, the Modular Bridge research is going on. Also performing cross-section optimization and duration reducing, this research aims at developing the modular bridge with Environment-Friendliness and economic feasibility. However, the difficulty lies in verifying environmental effectiveness because there are no field applications of the modular bridge until now. Therefore, this thesis is categorized according to the form of the modular bridge superstructure and assessed CO₂ emission quantification per work types and materials according to each form to verify the environmental effectiveness of the modular bridge. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=modular%20bridge" title="modular bridge">modular bridge</a>, <a href="https://publications.waset.org/abstracts/search?q=CO2%20emission" title=" CO2 emission"> CO2 emission</a>, <a href="https://publications.waset.org/abstracts/search?q=environmentally%20friendly" title=" environmentally friendly"> environmentally friendly</a>, <a href="https://publications.waset.org/abstracts/search?q=quantification" title=" quantification"> quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=carbon%20emission%20factor" title=" carbon emission factor"> carbon emission factor</a>, <a href="https://publications.waset.org/abstracts/search?q=LCA%20%28Life%20Cycle%20Assessment%29" title=" LCA (Life Cycle Assessment)"> LCA (Life Cycle Assessment)</a> </p> <a href="https://publications.waset.org/abstracts/28224/co2-emissions-quantification-of-the-modular-bridge-superstructure" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28224.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">555</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">525</span> Simultaneous Quantification of Glycols in New and Recycled Anti-Freeze Liquids by GC-MS</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=George%20Madalin%20Danila">George Madalin Danila</a>, <a href="https://publications.waset.org/abstracts/search?q=Mihaiella%20Cretu"> Mihaiella Cretu</a>, <a href="https://publications.waset.org/abstracts/search?q=Cristian%20Puscasu"> Cristian Puscasu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Glycol-based anti-freeze liquids, commonly composed of ethylene glycol or propylene glycol, have important uses in automotive cooling, but they should be handled with care due to their toxicity; ethylene glycol is highly toxic to humans and animals. A fast, accurate, precise, and robust method was developed for the simultaneous quantification of 7 most important glycols and their isomers. Glycols were analyzed from diluted sample solution of coolants using gas-chromatography coupled with mass spectrometry in single ion monitoring mode. Results: The method was developed and validated for 7 individual glycols (ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, propylene glycol, dipropylene glycol and tripropylene glycol). Limits of detection (1-2 μg/mL) and limit of quantification (10 μg/mL) obtained were appropriate. The present method was applied for the determination of glycols in 10 different anti-freeze liquids commercially available on the Romanian market, proving to be reliable. A method that requires only a two-step dilution of anti-freeze samples combined with direct liquid injection GC-MS was validated for the simultaneous quantification of 7 glycols (and their isomers) in 10 different types of anti-freeze liquids. The results obtained in the validation procedure proved that the GC-MS method is sensitive and precise for the quantification of glycols. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=glycols" title="glycols">glycols</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-freeze" title=" anti-freeze"> anti-freeze</a>, <a href="https://publications.waset.org/abstracts/search?q=gas-chromatography" title=" gas-chromatography"> gas-chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=validation" title=" validation"> validation</a>, <a href="https://publications.waset.org/abstracts/search?q=recycle" title=" recycle"> recycle</a> </p> <a href="https://publications.waset.org/abstracts/178902/simultaneous-quantification-of-glycols-in-new-and-recycled-anti-freeze-liquids-by-gc-ms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/178902.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">66</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">524</span> Quantification and Thermal Behavior of Rice Bran Oil, Sunflower Oil and Their Model Blends </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Harish%20Kumar%20Sharma">Harish Kumar Sharma</a>, <a href="https://publications.waset.org/abstracts/search?q=Garima%20Sengar"> Garima Sengar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rice bran oil is considered comparatively nutritionally superior than different fats/oils. Therefore, model blends prepared from pure rice bran oil (RBO) and sunflower oil (SFO) were explored for changes in the different physicochemical parameters. Repeated deep fat frying process was carried out by using dried potato in order to study the thermal behaviour of pure rice bran oil, sunflower oil and their model blends. Pure rice bran oil and sunflower oil had shown good thermal stability during the repeated deep fat frying cycles. Although, the model blends constituting 60% RBO + 40% SFO showed better suitability during repeated deep fat frying than the remaining blended oils. The quantification of pure rice bran oil in the blended oils, physically refined rice bran oil (PRBO): SnF (sunflower oil) was carried by different methods. The study revealed that regression equations based on the oryzanol content, palmitic acid composition and iodine value can be used for the quantification. The rice bran oil can easily be quantified in the blended oils based on the oryzanol content by HPLC even at 1% level. The palmitic acid content in blended oils can also be used as an indicator to quantify rice bran oil at or above 20% level in blended oils whereas the method based on ultrasonic velocity, acoustic impedance and relative association showed initial promise in the quantification. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rice%20bran%20oil" title="rice bran oil">rice bran oil</a>, <a href="https://publications.waset.org/abstracts/search?q=sunflower%20oil" title=" sunflower oil"> sunflower oil</a>, <a href="https://publications.waset.org/abstracts/search?q=frying" title=" frying"> frying</a>, <a href="https://publications.waset.org/abstracts/search?q=quantification" title=" quantification"> quantification</a> </p> <a href="https://publications.waset.org/abstracts/50592/quantification-and-thermal-behavior-of-rice-bran-oil-sunflower-oil-and-their-model-blends" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/50592.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">308</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">523</span> Disposable PANI-CeO2 Sensor for the Electrocatalytic Simultaneous Quantification of Amlodipine and Nebivolol </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nimisha%20Jadon">Nimisha Jadon</a>, <a href="https://publications.waset.org/abstracts/search?q=Rajeev%20Jain"> Rajeev Jain</a>, <a href="https://publications.waset.org/abstracts/search?q=Swati%20Sharma"> Swati Sharma</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A chemically modified carbon paste sensor has been developed for the simultaneous determination of amlodipine (AML) and nebivolol (NBV). Carbon paste electrode (CPE) was fabricated by the addition of Gr/PANI-CeO2. Gr/PANI-CeO2/CPE has achieved excellent electrocatalytic activity and sensitivity. AML and NBV exhibited oxidation peaks at 0.70 and 0.90 V respectively on Gr/ PANI-CeO2/CPE. The linearity range of AML and NBV was 0.1 to 1.6 μgmL-1 in BR buffer (pH 8.0). The Limit of detection (LOD) was 20.0 ngmL-1 for AML and 30.0 ngmL-1 for NBV and limit of quantification (LOQ) was 80.0 ngmL-1 for AML and 100 ngmL-1 for NBV respectively. These analyses were also determined in pharmaceutical formulation and human serum and good recovery was obtained for the developed method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=amlodipine" title="amlodipine">amlodipine</a>, <a href="https://publications.waset.org/abstracts/search?q=nebivolol" title=" nebivolol"> nebivolol</a>, <a href="https://publications.waset.org/abstracts/search?q=square%20wave%20voltammetry" title=" square wave voltammetry"> square wave voltammetry</a>, <a href="https://publications.waset.org/abstracts/search?q=carbon%20paste%20electrode" title=" carbon paste electrode"> carbon paste electrode</a>, <a href="https://publications.waset.org/abstracts/search?q=simultaneous%20quantification" title=" simultaneous quantification"> simultaneous quantification</a> </p> <a href="https://publications.waset.org/abstracts/80595/disposable-pani-ceo2-sensor-for-the-electrocatalytic-simultaneous-quantification-of-amlodipine-and-nebivolol" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80595.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">354</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">522</span> Identification and Quantification of Phenolic Compounds In Cassia tora Collected from Three Different Locations Using Ultra High Performance Liquid Chromatography – Electro Spray Ionization – Mass Spectrometry (UHPLC-ESI-MS-MS)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shipra%20Shukla">Shipra Shukla</a>, <a href="https://publications.waset.org/abstracts/search?q=Gaurav%20Chaudhary"> Gaurav Chaudhary</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20K.%20Tewari"> S. K. Tewari</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahesh%20Pal"> Mahesh Pal</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20K.%20Upreti"> D. K. Upreti</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cassia tora L. is widely distributed in tropical Asian countries, commonly known as sickle pod. Various parts of the plant are reported for their medicinal value due to presence of anthraquinones, phenolic compounds, emodin, β-sitosterol, and chrysophanol. Therefore a sensitive analytical procedure using UHPLC-ESI-MS/MS was developed and validated for simultaneous quantification of five phenolic compounds in leaf, stem and root extracts of Cassia tora. Rapid chromatographic separation of compounds was achieved on Acquity UHPLC BEH C18 column (50 mm×2.1 mm id, 1.7µm) column in 2.5 min. Quantification was carried out using negative electrospray ionization in multiple-reaction monitoring mode. The method was validated as per ICH guidelines and showed good linearity (r2 ≥ 0.9985) over the concentration range of 0.5-200 ng/mL. The intra- and inter-day precisions and accuracy were within RSDs ≤ 1.93% and ≤ 1.90%, respectively. The developed method was applied to investigate variation of five phenolic compounds in the three geographical collections. Results indicated significant variation among analyzed samples collected from different locations in India. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cassia%20tora" title="Cassia tora">Cassia tora</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20compounds" title=" phenolic compounds"> phenolic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=quantification" title=" quantification"> quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=UHPLC-ESI-MS%2FMS" title=" UHPLC-ESI-MS/MS"> UHPLC-ESI-MS/MS</a> </p> <a href="https://publications.waset.org/abstracts/56791/identification-and-quantification-of-phenolic-compounds-in-cassia-tora-collected-from-three-different-locations-using-ultra-high-performance-liquid-chromatography-electro-spray-ionization-mass-spectrometry-uhplc-esi-ms-ms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56791.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">269</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">521</span> Method Development and Validation for Quantification of Active Content and Impurities of Clodinafop Propargyl and Its Enantiomeric Separation by High-Performance Liquid Chromatography</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kamlesh%20Vishwakarma">Kamlesh Vishwakarma</a>, <a href="https://publications.waset.org/abstracts/search?q=Bipul%20Behari%20Saha"> Bipul Behari Saha</a>, <a href="https://publications.waset.org/abstracts/search?q=Sunilkumar%20Sing"> Sunilkumar Sing</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhishek%20Mishra"> Abhishek Mishra</a>, <a href="https://publications.waset.org/abstracts/search?q=Sreenivas%20Rao"> Sreenivas Rao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A rapid, sensitive and inexpensive method has been developed for complete analysis of Clodinafop Propargyl. Clodinafop Propargyl enantiomers were separated on chiral column, Chiral Pak AS-H (250 mm. 4.6mm x 5µm) with mobile phase n-hexane: IPA (96:4) at flow rate 1.5 ml/min. The effluent was monitored by UV detector at 230 nm. Clodinafop Propagyl content and impurity quantification was done with reverse phase HPLC. The present study describes a HPLC method using simple mobile phase for the quantification of Clodinafop Propargyl and its impurities. The method was validated and found to be accurate, precise, convenient and effective. Moreover, the lower solvent consumption along with short analytical run time led to a cost effective analytical method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Clodinafop%20Propargyl" title="Clodinafop Propargyl">Clodinafop Propargyl</a>, <a href="https://publications.waset.org/abstracts/search?q=method" title=" method"> method</a>, <a href="https://publications.waset.org/abstracts/search?q=validation" title=" validation"> validation</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC-UV" title=" HPLC-UV"> HPLC-UV</a> </p> <a href="https://publications.waset.org/abstracts/63561/method-development-and-validation-for-quantification-of-active-content-and-impurities-of-clodinafop-propargyl-and-its-enantiomeric-separation-by-high-performance-liquid-chromatography" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63561.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">371</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">520</span> Quantification of Uncertainties Related to the Implementation of Reverse Logistics Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dnaya%20Soukaina">Dnaya Soukaina</a> </p> <p class="card-text"><strong>Abstract:</strong></p> It’s over six decades that Reverse logistics had appeared as a research area, and it is emerging again and again in the scientific fields. As reverse logistics presents real potential for value recovery and environmental impacts decrease, it’s still necessary to extend this concept more in the industrial and commercial field especially in developing countries. The process of reverse logistics is a progression of steps beginning with the customer and finishing with the organization or even the customer, however the issue is that this cycle must be adjustable to the organization concerned, in addition of legislative, operational, financial and social obstacles. Literature had demonstrated that there are many other uncertainties while the implementation of this process that vary in function of the sector concerned and the kind of activity. Besides, even if literature is developing this topic over the last years, reseraches about uncertainties quantification in reverse logistics process still being few. the paper has the objective to fill this gap, and carry out a study to identify sustainable strategies that can be adapted to different industrial or commercial sectors to facilitate the implementation of reverse logistics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=reverse%20logistics" title="reverse logistics">reverse logistics</a>, <a href="https://publications.waset.org/abstracts/search?q=implementation" title=" implementation"> implementation</a>, <a href="https://publications.waset.org/abstracts/search?q=unceratinties%20quantification" title=" unceratinties quantification"> unceratinties quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=mathematical%20model" title=" mathematical model"> mathematical model</a> </p> <a href="https://publications.waset.org/abstracts/192110/quantification-of-uncertainties-related-to-the-implementation-of-reverse-logistics-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/192110.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">17</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">519</span> Simultaneous Extraction and Estimation of Steroidal Glycosides and Aglycone of Solanum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Karishma%20Chester">Karishma Chester</a>, <a href="https://publications.waset.org/abstracts/search?q=Sarvesh%20Paliwal"> Sarvesh Paliwal</a>, <a href="https://publications.waset.org/abstracts/search?q=Sayeed%20Ahmad"> Sayeed Ahmad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Solanumnigrum L. (Family: Solanaceae), is an important Indian medicinal plant and have been used in various traditional formulations for hepato-protection. It has been reported to contain significant amount of steroidal glycosides such as solamargine and solasonine as well as their aglycone part solasodine. Being important pharmacologically active metabolites of several members of Solanaceae these markers have been attempted various times for their extraction and quantification but separately for glycoside and aglycone part because of their opposite polarity. Here, we propose for the first time simultaneous extraction and quantification of aglycone (solasodine)and glycosides (solamargine and solasonine) inleaves and berries of S.nigrumusing solvent extraction followed by HPTLC analysis. Simultaneous extraction was carried out by sonication in mixture of chloroform and methanol as solvent. The quantification was done using silica gel 60F254HPTLC plates as stationary phase and chloroform: methanol: acetone: 0.5 % ammonia (7: 2.5: 1: 0.4 v/v/v/v) as mobile phaseat 400 nm, after derivatization with an isaldehydesul furic acid reagent. The method was validated as per ICH guideline for calibration, linearity, precision, recovery, robustness, specificity, LOD, and LOQ. The statistical data obtained for validation showed that method can be used routinely for quality control of various solanaceous drugs reported for these markers as well as traditional formulations containing those plants as an ingredient. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solanumnigrum" title="solanumnigrum">solanumnigrum</a>, <a href="https://publications.waset.org/abstracts/search?q=solasodine" title=" solasodine"> solasodine</a>, <a href="https://publications.waset.org/abstracts/search?q=solamargine" title=" solamargine"> solamargine</a>, <a href="https://publications.waset.org/abstracts/search?q=solasonine" title=" solasonine"> solasonine</a>, <a href="https://publications.waset.org/abstracts/search?q=quantification" title=" quantification"> quantification</a> </p> <a href="https://publications.waset.org/abstracts/3489/simultaneous-extraction-and-estimation-of-steroidal-glycosides-and-aglycone-of-solanum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3489.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">329</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">518</span> Quantification of Lustre in Textile Fibers by Image Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Neelesh%20Bharti%20Shukla">Neelesh Bharti Shukla</a>, <a href="https://publications.waset.org/abstracts/search?q=Suvankar%20Dutta"> Suvankar Dutta</a>, <a href="https://publications.waset.org/abstracts/search?q=Esha%20Sharma"> Esha Sharma</a>, <a href="https://publications.waset.org/abstracts/search?q=Shrikant%20Ralebhat"> Shrikant Ralebhat</a>, <a href="https://publications.waset.org/abstracts/search?q=Gurudatt%20Krishnamurthy"> Gurudatt Krishnamurthy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A key component of the physical attribute of textile fibers is lustre. It is a complex phenomenon arising from the interaction of light with fibers, yarn and fabrics. It is perceived as the contrast difference between the bright areas (specular reflection) and duller backgrounds (diffused reflection). Lustre of fibers is affected by their surface structure, morphology, cross-section profile as well as the presence of any additives/registrants. Due to complexities in measurements, objective measurements such as gloss meter do not give reproducible quantification of lustre. Other instruments such as SAMBA hair systems are expensive. In light of this, lustre quantification has largely remained subjective, judged visually by experts, but prone to errors. In this development, a physics-based approach was conceptualized and demonstrated. We have developed an image analysis based technique to quantify visually observed differences in lustre of fibers. Cellulosic fibers, produced with different approaches, with visually different levels of lustre were photographed under controlled optics. These images were subsequently analyzed using a configured software system. The ratio of Intensity of light from bright (specular reflection) and dull (diffused reflection) areas was used to numerically represent lustre. In the next step, the set of samples that were not visually distinguishable easily were also evaluated by the technique and it was established that quantification of lustre is feasible. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lustre" title="lustre">lustre</a>, <a href="https://publications.waset.org/abstracts/search?q=fibre" title=" fibre"> fibre</a>, <a href="https://publications.waset.org/abstracts/search?q=image%20analysis" title=" image analysis"> image analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=measurement" title=" measurement"> measurement</a> </p> <a href="https://publications.waset.org/abstracts/117528/quantification-of-lustre-in-textile-fibers-by-image-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/117528.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">169</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">517</span> Development and Validation of a Liquid Chromatographic Method for the Quantification of Related Substance in Gentamicin Drug Substances</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sofiqul%20Islam">Sofiqul Islam</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Murugan"> V. Murugan</a>, <a href="https://publications.waset.org/abstracts/search?q=Prema%20Kumari"> Prema Kumari</a>, <a href="https://publications.waset.org/abstracts/search?q=Hari"> Hari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gentamicin is a broad spectrum water-soluble aminoglycoside antibiotics produced by the fermentation process of microorganism known as Micromonospora purpurea. It is widely used for the treatment of infection caused by both gram positive and gram negative bacteria. Gentamicin consists of a mixture of aminoglycoside components like C1, C1a, C2a, and C2. The molecular structure of Gentamicin and its related substances showed that it has lack of presence of chromophore group in the molecule due to which the detection of such components were quite critical and challenging. In this study, a simple Reversed Phase-High Performance Liquid Chromatographic (RP-HPLC) method using ultraviolet (UV) detector was developed and validated for quantification of the related substances present in Gentamicin drug substances. The method was achieved by using Thermo Scientific Hypersil Gold analytical column (150 x 4.6 mm, 5 µm particle size) with isocratic elution composed of methanol: water: glacial acetic acid: sodium hexane sulfonate in the ratio 70:25:5:3 % v/v/v/w as a mobile phase at a flow rate of 0.5 mL/min, column temperature was maintained at 30 °C and detection wavelength of 330 nm. The four components of Gentamicin namely Gentamicin C1, C1a, C2a, and C2 were well separated along with the related substance present in Gentamicin. The Limit of Quantification (LOQ) values were found to be at 0.0075 mg/mL. The accuracy of the method was quite satisfactory in which the % recovery was resulted between 95-105% for the related substances. The correlation coefficient (≥ 0.995) shows the linearity response against concentration over the range of Limit of Quantification (LOQ). Precision studies showed the % Relative Standard Deviation (RSD) values less than 5% for its related substance. The method was validated in accordance with the International Conference of Harmonization (ICH) guideline with various parameters like system suitability, specificity, precision, linearity, accuracy, limit of quantification, and robustness. This proposed method was easy and suitable for use for the quantification of related substances in routine analysis of Gentamicin formulations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=reversed%20phase-high%20performance%20liquid%20chromatographic%20%28RP-HPLC%29" title="reversed phase-high performance liquid chromatographic (RP-HPLC)">reversed phase-high performance liquid chromatographic (RP-HPLC)</a>, <a href="https://publications.waset.org/abstracts/search?q=high%20performance%20liquid%20chromatography" title=" high performance liquid chromatography"> high performance liquid chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=gentamicin" title=" gentamicin"> gentamicin</a>, <a href="https://publications.waset.org/abstracts/search?q=isocratic" title=" isocratic"> isocratic</a>, <a href="https://publications.waset.org/abstracts/search?q=ultraviolet" title=" ultraviolet"> ultraviolet</a> </p> <a href="https://publications.waset.org/abstracts/97993/development-and-validation-of-a-liquid-chromatographic-method-for-the-quantification-of-related-substance-in-gentamicin-drug-substances" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/97993.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">160</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">516</span> Quality by Design in the Optimization of a Fast HPLC Method for Quantification of Hydroxychloroquine Sulfate</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pedro%20J.%20Rolim-Neto">Pedro J. Rolim-Neto</a>, <a href="https://publications.waset.org/abstracts/search?q=Leslie%20R.%20M.%20Ferraz"> Leslie R. M. Ferraz</a>, <a href="https://publications.waset.org/abstracts/search?q=Fabiana%20L.%20A.%20Santos"> Fabiana L. A. Santos</a>, <a href="https://publications.waset.org/abstracts/search?q=Pablo%20A.%20Ferreira"> Pablo A. Ferreira</a>, <a href="https://publications.waset.org/abstracts/search?q=Ricardo%20T.%20L.%20Maia-Jr."> Ricardo T. L. Maia-Jr.</a>, <a href="https://publications.waset.org/abstracts/search?q=Magaly%20A.%20M.%20Lyra"> Magaly A. M. Lyra</a>, <a href="https://publications.waset.org/abstracts/search?q=Danilo%20A%20F.%20Fonte"> Danilo A F. Fonte</a>, <a href="https://publications.waset.org/abstracts/search?q=Salvana%20P.%20M.%20Costa"> Salvana P. M. Costa</a>, <a href="https://publications.waset.org/abstracts/search?q=Amanda%20C.%20Q.%20M.%20Vieira"> Amanda C. Q. M. Vieira</a>, <a href="https://publications.waset.org/abstracts/search?q=Larissa%20A.%20Rolim"> Larissa A. Rolim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Initially developed as an antimalarial agent, hydroxychloroquine (HCQ) sulfate is often used as a slow-acting antirheumatic drug in the treatment of disorders of connective tissue. The United States Pharmacopeia (USP) 37 provides a reversed-phase HPLC method for quantification of HCQ. However, this method was not reproducible, producing asymmetric peaks in a long analysis time. The asymmetry of the peak may cause an incorrect calculation of the concentration of the sample. Furthermore, the analysis time is unacceptable, especially regarding the routine of a pharmaceutical industry. The aiming of this study was to develop a fast, easy and efficient method for quantification of HCQ sulfate by High Performance Liquid Chromatography (HPLC) based on the Quality by Design (QbD) methodology. This method was optimized in terms of peak symmetry using the surface area graphic as the Design of Experiments (DoE) and the tailing factor (TF) as an indicator to the Design Space (DS). The reference method used was that described at USP 37 to the quantification of the drug. For the optimized method, was proposed a 33 factorial design, based on the QbD concepts. The DS was created with the TF (in a range between 0.98 and 1.2) in order to demonstrate the ideal analytical conditions. Changes were made in the composition of the USP mobile-phase (USP-MP): USP-MP: Methanol (90:10 v/v, 80:20 v/v and 70:30 v/v), in the flow (0.8, 1.0 and 1.2 mL) and in the oven temperature (30, 35, and 40ºC). The USP method allowed the quantification of drug in a long time (40-50 minutes). In addition, the method uses a high flow rate (1,5 mL.min-1) which increases the consumption of expensive solvents HPLC grade. The main problem observed was the TF value (1,8) that would be accepted if the drug was not a racemic mixture, since the co-elution of the isomers can become an unreliable peak integration. Therefore, the optimization was suggested in order to reduce the analysis time, aiming a better peak resolution and TF. For the optimization method, by the analysis of the surface-response plot it was possible to confirm the ideal setting analytical condition: 45 °C, 0,8 mL.min-1 and 80:20 USP-MP: Methanol. The optimized HPLC method enabled the quantification of HCQ sulfate, with a peak of high resolution, showing a TF value of 1,17. This promotes good co-elution of isomers of the HCQ, ensuring an accurate quantification of the raw material as racemic mixture. This method also proved to be 18 times faster, approximately, compared to the reference method, using a lower flow rate, reducing even more the consumption of the solvents and, consequently, the analysis cost. Thus, an analytical method for the quantification of HCQ sulfate was optimized using QbD methodology. This method proved to be faster and more efficient than the USP method, regarding the retention time and, especially, the peak resolution. The higher resolution in the chromatogram peaks supports the implementation of the method for quantification of the drug as racemic mixture, not requiring the separation of isomers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=analytical%20method" title="analytical method">analytical method</a>, <a href="https://publications.waset.org/abstracts/search?q=hydroxychloroquine%20sulfate" title=" hydroxychloroquine sulfate"> hydroxychloroquine sulfate</a>, <a href="https://publications.waset.org/abstracts/search?q=quality%20by%20design" title=" quality by design"> quality by design</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20area%20graphic" title=" surface area graphic"> surface area graphic</a> </p> <a href="https://publications.waset.org/abstracts/25658/quality-by-design-in-the-optimization-of-a-fast-hplc-method-for-quantification-of-hydroxychloroquine-sulfate" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25658.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">639</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">515</span> Dow Polyols near Infrared Chemometric Model Reduction Based on Clustering: Reducing Thirty Global Hydroxyl Number (OH) Models to Less Than Five</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wendy%20Flory">Wendy Flory</a>, <a href="https://publications.waset.org/abstracts/search?q=Kazi%20Czarnecki"> Kazi Czarnecki</a>, <a href="https://publications.waset.org/abstracts/search?q=Matthijs%20Mercy"> Matthijs Mercy</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20Joswiak"> Mark Joswiak</a>, <a href="https://publications.waset.org/abstracts/search?q=Mary%20Beth%20Seasholtz"> Mary Beth Seasholtz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Polyurethane Materials are present in a wide range of industrial segments such as Furniture, Building and Construction, Composites, Automotive, Electronics, and more. Dow is one of the leaders for the manufacture of the two main raw materials, Isocyanates and Polyols used to produce polyurethane products. Dow is also a key player for the manufacture of Polyurethane Systems/Formulations designed for targeted applications. In 1990, the first analytical chemometric models were developed and deployed for use in the Dow QC labs of the polyols business for the quantification of OH, water, cloud point, and viscosity. Over the years many models have been added; there are now over 140 models for quantification and hundreds for product identification, too many to be reasonable for support. There are 29 global models alone for the quantification of OH across > 70 products at many sites. An attempt was made to consolidate these into a single model. While the consolidated model proved good statistics across the entire range of OH, several products had a bias by ASTM E1655 with individual product validation. This project summary will show the strategy for global model updates for OH, to reduce the number of models for quantification from over 140 to 5 or less using chemometric methods. In order to gain an understanding of the best product groupings, we identify clusters by reducing spectra to a few dimensions via Principal Component Analysis (PCA) and Uniform Manifold Approximation and Projection (UMAP). Results from these cluster analyses and a separate validation set allowed dow to reduce the number of models for predicting OH from 29 to 3 without loss of accuracy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hydroxyl" title="hydroxyl">hydroxyl</a>, <a href="https://publications.waset.org/abstracts/search?q=global%20model" title=" global model"> global model</a>, <a href="https://publications.waset.org/abstracts/search?q=model%20maintenance" title=" model maintenance"> model maintenance</a>, <a href="https://publications.waset.org/abstracts/search?q=near%20infrared" title=" near infrared"> near infrared</a>, <a href="https://publications.waset.org/abstracts/search?q=polyol" title=" polyol"> polyol</a> </p> <a href="https://publications.waset.org/abstracts/107706/dow-polyols-near-infrared-chemometric-model-reduction-based-on-clustering-reducing-thirty-global-hydroxyl-number-oh-models-to-less-than-five" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/107706.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">135</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">514</span> Approach to Quantify Groundwater Recharge Using GIS Based Water Balance Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20S.%20Rwanga">S. S. Rwanga</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20M.%20Ndambuki"> J. M. Ndambuki</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Groundwater quantification needs a method which is not only flexible but also reliable in order to accurately quantify its spatial and temporal variability. As groundwater is dynamic and interdisciplinary in nature, an integrated approach of remote sensing (RS) and GIS technique is very useful in various groundwater management studies. Thus, the GIS water balance model (WetSpass) together with remote sensing (RS) can be used to quantify groundwater recharge. This paper discusses the concept of WetSpass in combination with GIS on the quantification of recharge with a view to managing water resources in an integrated framework. The paper presents the simulation procedures and expected output after simulation. Preliminary data are presented from GIS output only. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=groundwater" title="groundwater">groundwater</a>, <a href="https://publications.waset.org/abstracts/search?q=recharge" title=" recharge"> recharge</a>, <a href="https://publications.waset.org/abstracts/search?q=GIS" title=" GIS"> GIS</a>, <a href="https://publications.waset.org/abstracts/search?q=WetSpass" title=" WetSpass"> WetSpass</a> </p> <a href="https://publications.waset.org/abstracts/33834/approach-to-quantify-groundwater-recharge-using-gis-based-water-balance-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/33834.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">450</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">513</span> Comparison Between a Droplet Digital PCR and Real Time PCR Method in Quantification of HBV DNA</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Surangrat%20Srisurapanon">Surangrat Srisurapanon</a>, <a href="https://publications.waset.org/abstracts/search?q=Chatchawal%20Wongjitrat"> Chatchawal Wongjitrat</a>, <a href="https://publications.waset.org/abstracts/search?q=Navin%20Horthongkham"> Navin Horthongkham</a>, <a href="https://publications.waset.org/abstracts/search?q=Ruengpung%20Sutthent"> Ruengpung Sutthent </a> </p> <p class="card-text"><strong>Abstract:</strong></p> HBV infection causes a potential serious public health problem. The ability to detect the HBV DNA concentration is of the importance and improved continuously. By using quantitative Polymerase Chain Reaction (qPCR), several factors in standardized; source of material, calibration standard curve and PCR efficiency are inconsistent. Digital PCR (dPCR) is an alternative PCR-based technique for absolute quantification using Poisson's statistics without requiring a standard curve. Therefore, the aim of this study is to compare the data set of HBV DNA generated between dPCR and qPCR methods. All samples were quantified by Abbott’s real time PCR and 54 samples with 2 -6 log10 HBV DNA were selected for comparison with dPCR. Of these 54 samples, there were two outlier samples defined as negative by dPCR. Of these two, samples were defined as negative by dPCR, whereas 52 samples were positive by both the tests. The difference between the two assays was less than 0.25 log IU/mL in 24/52 samples (46%) of paired samples; less than 0.5 log IU/mL in 46/52 samples (88%) and less than 1 log in 50/52 samples (96%). The correlation coefficient was r=0.788 and P-value <0.0001. Comparison to qPCR, data generated by dPCR tend to be the overestimation in the sample with low HBV DNA concentration and underestimated in the sample with high viral load. The variation in DNA by dPCR measurement might be due to the pre-amplification bias, template. Moreover, a minor drawback of dPCR is the large quantity of DNA had to be used when compare to the qPCR. Since the technology is relatively new, the limitations of this assay will be improved. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hepatitis%20B%20virus" title="hepatitis B virus">hepatitis B virus</a>, <a href="https://publications.waset.org/abstracts/search?q=real%20time%20PCR" title=" real time PCR"> real time PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=digital%20PCR" title=" digital PCR"> digital PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20quantification" title=" DNA quantification"> DNA quantification</a> </p> <a href="https://publications.waset.org/abstracts/25135/comparison-between-a-droplet-digital-pcr-and-real-time-pcr-method-in-quantification-of-hbv-dna" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25135.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">481</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">512</span> Development of Colorimetric Based Microfluidic Platform for Quantification of Fluid Contaminants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sangeeta%20Palekar">Sangeeta Palekar</a>, <a href="https://publications.waset.org/abstracts/search?q=Mahima%20Rana"> Mahima Rana</a>, <a href="https://publications.waset.org/abstracts/search?q=Jayu%20Kalambe"> Jayu Kalambe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, a microfluidic-based platform for the quantification of contaminants in the water is proposed. The proposed system uses microfluidic channels with an embedded environment for contaminants detection in water. Microfluidics-based platforms present an evident stage of innovation for fluid analysis, with different applications advancing minimal efforts and simplicity of fabrication. Polydimethylsiloxane (PDMS)-based microfluidics channel is fabricated using a soft lithography technique. Vertical and horizontal connections for fluid dispensing with the microfluidic channel are explored. The principle of colorimetry, which incorporates the use of Griess reagent for the detection of nitrite, has been adopted. Nitrite has high water solubility and water retention, due to which it has a greater potential to stay in groundwater, endangering aquatic life along with human health, hence taken as a case study in this work. The developed platform also compares the detection methodology, containing photodetectors for measuring absorbance and image sensors for measuring color change for quantification of contaminants like nitrite in water. The utilization of image processing techniques offers the advantage of operational flexibility, as the same system can be used to identify other contaminants present in water by introducing minor software changes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=colorimetric" title="colorimetric">colorimetric</a>, <a href="https://publications.waset.org/abstracts/search?q=fluid%20contaminants" title=" fluid contaminants"> fluid contaminants</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrite%20detection" title=" nitrite detection"> nitrite detection</a>, <a href="https://publications.waset.org/abstracts/search?q=microfluidics" title=" microfluidics"> microfluidics</a> </p> <a href="https://publications.waset.org/abstracts/141028/development-of-colorimetric-based-microfluidic-platform-for-quantification-of-fluid-contaminants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141028.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">198</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">511</span> Quantification of Learned Non-Use of the Upper-Limb After a Stroke</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20K.%20A.%20Bakhti">K. K. A. Bakhti</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Mottet"> D. Mottet</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Froger"> J. Froger</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Laffont"> I. Laffont</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: After a cerebrovascular accident (or stroke), many patients use excessive trunk movements to move their paretic hand towards a target (while the elbow is maintained flexed) even though they can use the upper-limb when the trunk is restrained. This phenomenon is labelled learned non-use and is known to be detrimental to neuroplasticity and recovery. Objective: The aim of this study is to quantify learned non-use of the paretic upper limb during a hand reaching task using 3D movement analysis. Methods: Thirty-four participants post supratentorial stroke were asked to reach a cone placed in front of them at 80% of their arm length. The reaching movement was repeated 5 times with the paretic hand, and then 5 times with the less-impaired hand. This sequence was first performed with the trunk free, then with the trunk restrained. Learned non-use of the upper-limb (LNUUL) was obtained from the difference of the amount of trunk compensation between the free trunk condition and the restrained trunk condition. Results: LNUUL was significantly higher for the paretic hand, with individual values ranging from 1% to 43%, and one-half of the patients with an LNUUL higher than 15%. Conclusions: Quantification of LNUUL can be used to objectively diagnose patients who need trunk rehabilitation. It can be also used for monitoring the rehabilitation progress. Quantification of LNUUL may guide upper-limb rehabilitation towards more optimal motor recovery avoiding maladaptive trunk compensation and its consequences on neuroplasticity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=learned%20non-use" title="learned non-use">learned non-use</a>, <a href="https://publications.waset.org/abstracts/search?q=rehabilitation" title=" rehabilitation"> rehabilitation</a>, <a href="https://publications.waset.org/abstracts/search?q=stroke" title=" stroke"> stroke</a>, <a href="https://publications.waset.org/abstracts/search?q=upper%20limb" title=" upper limb"> upper limb</a> </p> <a href="https://publications.waset.org/abstracts/32555/quantification-of-learned-non-use-of-the-upper-limb-after-a-stroke" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32555.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">238</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">510</span> HPTLC Based Qualitative and Quantitative Evaluation of Uraria picta Desv: A Dashmool Species</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hari%20O.%20Saxena">Hari O. Saxena</a>, <a href="https://publications.waset.org/abstracts/search?q=Ganesh"> Ganesh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present investigation, chemical fingerprints of methanolic extracts of roots, stem and leaves of Uraria picta were developed using HPTLC technique. These fingerprints will be useful for authentication as well as in differentiating the species from adulterants. These will also serve as a biochemical marker for this valuable species in pharmaceutical industries and plant systemic studies. Roots, stem and leaves of Uraria picta were further evaluated for quantification of an active ingredient lupeol to find out alternatives to roots. Results showed more content of lupeol in stem (0.048%, dry wt.) as compare to roots (0.017%, dry wt.) suggesting the utilization of stem in place of roots. It will avoid uprooting of this prestigious plant which ultimately will promote its conservation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chemical%20fingerprints" title="chemical fingerprints">chemical fingerprints</a>, <a href="https://publications.waset.org/abstracts/search?q=lupeol" title=" lupeol"> lupeol</a>, <a href="https://publications.waset.org/abstracts/search?q=quantification" title=" quantification"> quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=Uraria%20picta" title=" Uraria picta"> Uraria picta</a> </p> <a href="https://publications.waset.org/abstracts/54865/hptlc-based-qualitative-and-quantitative-evaluation-of-uraria-picta-desv-a-dashmool-species" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54865.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">255</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">509</span> Investigation of Complexity Dynamics in a DC Glow Discharge Magnetized Plasma Using Recurrence Quantification Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vramori%20Mitra">Vramori Mitra</a>, <a href="https://publications.waset.org/abstracts/search?q=Bornali%20Sarma"> Bornali Sarma</a>, <a href="https://publications.waset.org/abstracts/search?q=Arun%20K.%20Sarma"> Arun K. Sarma</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recurrence is a ubiquitous feature of any real dynamical system. The states in phase space trajectory of a system have an inherent tendency to return to the same state or its close state after certain time laps. Recurrence quantification analysis technique, based on this fundamental feature of a dynamical system, detects evaluation of state under variation of control parameter of the system. The paper presents the investigation of nonlinear dynamical behavior of plasma floating potential fluctuations obtained by using a Langmuir probe in different magnetic field under the variation of discharge voltages. The main measures of recurrence quantification analysis are considered as determinism, linemax and entropy. The increment of the DET and linemax variables asserts that the predictability and periodicity of the system is increasing. The variable linemax indicates that the chaoticity is being diminished with the slump of magnetic field while increase of magnetic field enhancing the chaotic behavior. Fractal property of the plasma time series estimated by DFA technique (Detrended fluctuation analysis) reflects that long-range correlation of plasma fluctuations is decreasing while fractal dimension is increasing with the enhancement of magnetic field which corroborates the RQA analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=detrended%20fluctuation%20analysis" title="detrended fluctuation analysis">detrended fluctuation analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=chaos" title=" chaos"> chaos</a>, <a href="https://publications.waset.org/abstracts/search?q=phase%20space" title=" phase space"> phase space</a>, <a href="https://publications.waset.org/abstracts/search?q=recurrence" title=" recurrence"> recurrence</a> </p> <a href="https://publications.waset.org/abstracts/42471/investigation-of-complexity-dynamics-in-a-dc-glow-discharge-magnetized-plasma-using-recurrence-quantification-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42471.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">328</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">508</span> Video-Based System for Support of Robot-Enhanced Gait Rehabilitation of Stroke Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Matja%C5%BE%20Divjak">Matjaž Divjak</a>, <a href="https://publications.waset.org/abstracts/search?q=Simon%20Zeli%C4%8D"> Simon Zelič</a>, <a href="https://publications.waset.org/abstracts/search?q=Ale%C5%A1%20Holobar"> Aleš Holobar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We present a dedicated video-based monitoring system for quantification of patient’s attention to visual feedback during robot assisted gait rehabilitation. Two different approaches for eye gaze and head pose tracking are tested and compared. Several metrics for assessment of patient’s attention are also presented. Experimental results with healthy volunteers demonstrate that unobtrusive video-based gaze tracking during the robot-assisted gait rehabilitation is possible and is sufficiently robust for quantification of patient’s attention and assessment of compliance with the rehabilitation therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=video-based%20attention%20monitoring" title="video-based attention monitoring">video-based attention monitoring</a>, <a href="https://publications.waset.org/abstracts/search?q=gaze%20estimation" title=" gaze estimation"> gaze estimation</a>, <a href="https://publications.waset.org/abstracts/search?q=stroke%20rehabilitation" title=" stroke rehabilitation"> stroke rehabilitation</a>, <a href="https://publications.waset.org/abstracts/search?q=user%20compliance" title=" user compliance"> user compliance</a> </p> <a href="https://publications.waset.org/abstracts/11930/video-based-system-for-support-of-robot-enhanced-gait-rehabilitation-of-stroke-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/11930.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">424</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">507</span> Microstructural Investigation and Fatigue Damage Quantification of Anisotropic Behavior in AA2017 Aluminum Alloy under Cyclic Loading</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdelghani%20May">Abdelghani May</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper reports on experimental investigations concerning the underlying reasons for the anisotropic behavior observed during the cyclic loading of AA2017 aluminum alloy. Initially, we quantified the evolution of fatigue damage resulting from controlled proportional cyclic loadings along the axial and shear directions. Our primary objective at this stage was to verify the anisotropic mechanical behavior recently observed. To accomplish this, we utilized various models of fatigue damage quantification and conducted a comparative study of the obtained results. Our analysis confirmed the anisotropic nature of the material under investigation. In the subsequent step, we performed microstructural investigations aimed at understanding the origins of the anisotropic mechanical behavior. To this end, we utilized scanning electron microscopy to examine the phases and precipitates in both the transversal and longitudinal sections. Our findings indicate that the structure and morphology of these entities are responsible for the anisotropic behavior observed in the aluminum alloy. Furthermore, results obtained from Kikuchi diagrams, pole figures, and inverse pole figures have corroborated these conclusions. These findings demonstrate significant differences in the crystallographic texture of the material. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microstructural%20investigation" title="microstructural investigation">microstructural investigation</a>, <a href="https://publications.waset.org/abstracts/search?q=fatigue%20damage%20quantification" title=" fatigue damage quantification"> fatigue damage quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=anisotropic%20behavior" title=" anisotropic behavior"> anisotropic behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=AA2017%20aluminum%20alloy" title=" AA2017 aluminum alloy"> AA2017 aluminum alloy</a>, <a href="https://publications.waset.org/abstracts/search?q=cyclic%20loading" title=" cyclic loading"> cyclic loading</a>, <a href="https://publications.waset.org/abstracts/search?q=crystallographic%20texture" title=" crystallographic texture"> crystallographic texture</a>, <a href="https://publications.waset.org/abstracts/search?q=scanning%20electron%20microscopy" title=" scanning electron microscopy"> scanning electron microscopy</a> </p> <a href="https://publications.waset.org/abstracts/165414/microstructural-investigation-and-fatigue-damage-quantification-of-anisotropic-behavior-in-aa2017-aluminum-alloy-under-cyclic-loading" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165414.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">506</span> Fast Prototyping of Precise, Flexible, Multiplexed, Printed Electrochemical Enzyme-Linked Immunosorbent Assay System for Point-of-Care Biomarker Quantification</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zahrasadat%20Hosseini">Zahrasadat Hosseini</a>, <a href="https://publications.waset.org/abstracts/search?q=Jie%20Yuan"> Jie Yuan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Point-of-care (POC) diagnostic devices based on lab-on-a-chip (LOC) technology have the potential to revolutionize medical diagnostics. However, the development of an ideal microfluidic system based on LOC technology for diagnostics purposes requires overcoming several obstacles, such as improving sensitivity, selectivity, portability, cost-effectiveness, and prototyping methods. While numerous studies have introduced technologies and systems that advance these criteria, existing systems still have limitations. Electrochemical enzyme-linked immunosorbent assay (e-ELISA) in a LOC device offers numerous advantages, including enhanced sensitivity, decreased turnaround time, minimized sample and analyte consumption, reduced cost, disposability, and suitability for miniaturization, integration, and multiplexing. In this study, we present a novel design and fabrication method for a microfluidic diagnostic platform that integrates screen-printed electrochemical carbon/silver chloride electrodes on flexible printed circuit boards with flexible, multilayer, polydimethylsiloxane (PDMS) microfluidic networks to accurately manipulate and pre-immobilize analytes for performing electrochemical enzyme-linked immunosorbent assay (e-ELISA) for multiplexed quantification of blood serum biomarkers. We further demonstrate fast, cost-effective prototyping, as well as accurate and reliable detection performance of this device for quantification of interleukin-6-spiked samples through electrochemical analytics methods. We anticipate that our invention represents a significant step towards the development of user-friendly, portable, medical-grade, POC diagnostic devices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lab-on-a-chip" title="lab-on-a-chip">lab-on-a-chip</a>, <a href="https://publications.waset.org/abstracts/search?q=point-of-care%20diagnostics" title=" point-of-care diagnostics"> point-of-care diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20ELISA" title=" electrochemical ELISA"> electrochemical ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=biomarker%20quantification" title=" biomarker quantification"> biomarker quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=fast%20prototyping" title=" fast prototyping"> fast prototyping</a> </p> <a href="https://publications.waset.org/abstracts/169509/fast-prototyping-of-precise-flexible-multiplexed-printed-electrochemical-enzyme-linked-immunosorbent-assay-system-for-point-of-care-biomarker-quantification" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/169509.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">83</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">505</span> Fast Prototyping of Precise, Flexible, Multiplexed, Printed Electrochemical Enzyme-Linked Immunosorbent Assay Platform for Point-of-Care Biomarker Quantification</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zahrasadat%20Hosseini">Zahrasadat Hosseini</a>, <a href="https://publications.waset.org/abstracts/search?q=Jie%20Yuan"> Jie Yuan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Point-of-care (POC) diagnostic devices based on lab-on-a-chip (LOC) technology have the potential to revolutionize medical diagnostics. However, the development of an ideal microfluidic system based on LOC technology for diagnostics purposes requires overcoming several obstacles, such as improving sensitivity, selectivity, portability, cost-effectiveness, and prototyping methods. While numerous studies have introduced technologies and systems that advance these criteria, existing systems still have limitations. Electrochemical enzyme-linked immunosorbent assay (e-ELISA) in a LOC device offers numerous advantages, including enhanced sensitivity, decreased turnaround time, minimized sample and analyte consumption, reduced cost, disposability, and suitability for miniaturization, integration, and multiplexing. In this study, we present a novel design and fabrication method for a microfluidic diagnostic platform that integrates screen-printed electrochemical carbon/silver chloride electrodes on flexible printed circuit boards with flexible, multilayer, polydimethylsiloxane (PDMS) microfluidic networks to accurately manipulate and pre-immobilize analytes for performing electrochemical enzyme-linked immunosorbent assay (e-ELISA) for multiplexed quantification of blood serum biomarkers. We further demonstrate fast, cost-effective prototyping, as well as accurate and reliable detection performance of this device for quantification of interleukin-6-spiked samples through electrochemical analytics methods. We anticipate that our invention represents a significant step towards the development of user-friendly, portable, medical-grade POC diagnostic devices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lab-on-a-chip" title="lab-on-a-chip">lab-on-a-chip</a>, <a href="https://publications.waset.org/abstracts/search?q=point-of-care%20diagnostics" title=" point-of-care diagnostics"> point-of-care diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20ELISA" title=" electrochemical ELISA"> electrochemical ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=biomarker%20quantification" title=" biomarker quantification"> biomarker quantification</a>, <a href="https://publications.waset.org/abstracts/search?q=fast%20prototyping" title=" fast prototyping"> fast prototyping</a> </p> <a href="https://publications.waset.org/abstracts/169508/fast-prototyping-of-precise-flexible-multiplexed-printed-electrochemical-enzyme-linked-immunosorbent-assay-platform-for-point-of-care-biomarker-quantification" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/169508.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">85</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">504</span> Standardization of a Methodology for Quantification of Antimicrobials Used for the Treatment of Multi-Resistant Bacteria Using Two Types of Biosensors and Production of Anti-Antimicrobial Antibodies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Garzon%20V.">Garzon V.</a>, <a href="https://publications.waset.org/abstracts/search?q=Bustos%20R."> Bustos R.</a>, <a href="https://publications.waset.org/abstracts/search?q=Salvador%20J.%20P."> Salvador J. P.</a>, <a href="https://publications.waset.org/abstracts/search?q=Marco%20M.%20P."> Marco M. P.</a>, <a href="https://publications.waset.org/abstracts/search?q=Pinacho%20D.%20G."> Pinacho D. G.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bacterial resistance to antimicrobial treatment has increased significantly in recent years, making it a public health problem. Large numbers of bacteria are resistant to all or nearly all known antimicrobials, creating the need for the development of new types of antimicrobials or the use of “last line” antimicrobial drug therapies for the treatment of multi-resistant bacteria. Some of the chemical groups of antimicrobials most used for the treatment of infections caused by multiresistant bacteria in the clinic are Glycopeptide (Vancomycin), Polymyxin (Colistin), Lipopeptide (Daptomycin) and Carbapenem (Meropenem). Molecules that require therapeutic drug monitoring (TDM). Due to the above, a methodology based on nanobiotechnology based on an optical and electrochemical biosensor is being developed, which allows the evaluation of the plasmatic levels of some antimicrobials such as glycopeptide, polymyxin, lipopeptide and carbapenem quickly, at a low cost, with a high specificity and sensitivity and that can be implemented in the future in public and private health hospitals. For this, the project was divided into five steps i) Design of specific anti-drug antibodies, produced in rabbits for each of the types of antimicrobials, evaluating the results by means of an immunoassay analysis (ELISA); ii) quantification by means of an electrochemical biosensor that allows quantification with high sensitivity and selectivity of the reference antimicrobials; iii) Comparison of antimicrobial quantification with an optical type biosensor; iv) Validation of the methodologies used with biosensor by means of an immunoassay. Finding as a result that it is possible to quantify antibiotics by means of the optical and electrochemical biosensor at concentrations on average of 1,000ng/mL, the antibodies being sensitive and specific for each of the antibiotic molecules, results that were compared with immunoassays and HPLC chromatography. Thus, contributing to the safe use of these drugs commonly used in clinical practice and new antimicrobial drugs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibiotics" title="antibiotics">antibiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20biosensor" title=" electrochemical biosensor"> electrochemical biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=optical%20biosensor" title=" optical biosensor"> optical biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=therapeutic%20drug%20monitoring" title=" therapeutic drug monitoring"> therapeutic drug monitoring</a> </p> <a href="https://publications.waset.org/abstracts/163528/standardization-of-a-methodology-for-quantification-of-antimicrobials-used-for-the-treatment-of-multi-resistant-bacteria-using-two-types-of-biosensors-and-production-of-anti-antimicrobial-antibodies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163528.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">82</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">503</span> Skull Extraction for Quantification of Brain Volume in Magnetic Resonance Imaging of Multiple Sclerosis Patients</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marcela%20De%20Oliveira">Marcela De Oliveira</a>, <a href="https://publications.waset.org/abstracts/search?q=Marina%20P.%20Da%20Silva"> Marina P. Da Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Fernando%20C.%20G.%20Da%20Rocha"> Fernando C. G. Da Rocha</a>, <a href="https://publications.waset.org/abstracts/search?q=Jorge%20M.%20Santos"> Jorge M. Santos</a>, <a href="https://publications.waset.org/abstracts/search?q=Jaime%20S.%20Cardoso"> Jaime S. Cardoso</a>, <a href="https://publications.waset.org/abstracts/search?q=Paulo%20N.%20Lisboa-Filho"> Paulo N. Lisboa-Filho</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Multiple Sclerosis (MS) is an immune-mediated disease of the central nervous system characterized by neurodegeneration, inflammation, demyelination, and axonal loss. Magnetic resonance imaging (MRI), due to the richness in the information details provided, is the gold standard exam for diagnosis and follow-up of neurodegenerative diseases, such as MS. Brain atrophy, the gradual loss of brain volume, is quite extensive in multiple sclerosis, nearly 0.5-1.35% per year, far off the limits of normal aging. Thus, the brain volume quantification becomes an essential task for future analysis of the occurrence atrophy. The analysis of MRI has become a tedious and complex task for clinicians, who have to manually extract important information. This manual analysis is prone to errors and is time consuming due to various intra- and inter-operator variability. Nowadays, computerized methods for MRI segmentation have been extensively used to assist doctors in quantitative analyzes for disease diagnosis and monitoring. Thus, the purpose of this work was to evaluate the brain volume in MRI of MS patients. We used MRI scans with 30 slices of the five patients diagnosed with multiple sclerosis according to the McDonald criteria. The computational methods for the analysis of images were carried out in two steps: segmentation of the brain and brain volume quantification. The first image processing step was to perform brain extraction by skull stripping from the original image. In the skull stripper for MRI images of the brain, the algorithm registers a grayscale atlas image to the grayscale patient image. The associated brain mask is propagated using the registration transformation. Then this mask is eroded and used for a refined brain extraction based on level-sets (edge of the brain-skull border with dedicated expansion, curvature, and advection terms). In the second step, the brain volume quantification was performed by counting the voxels belonging to the segmentation mask and converted in cc. We observed an average brain volume of 1469.5 cc. We concluded that the automatic method applied in this work can be used for the brain extraction process and brain volume quantification in MRI. The development and use of computer programs can contribute to assist health professionals in the diagnosis and monitoring of patients with neurodegenerative diseases. In future works, we expect to implement more automated methods for the assessment of cerebral atrophy and brain lesions quantification, including machine-learning approaches. Acknowledgements: This work was supported by a grant from Brazilian agency Fundação de Amparo à Pesquisa do Estado de São Paulo (number 2019/16362-5). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=brain%20volume" title="brain volume">brain volume</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic%20resonance%20imaging" title=" magnetic resonance imaging"> magnetic resonance imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=multiple%20sclerosis" title=" multiple sclerosis"> multiple sclerosis</a>, <a href="https://publications.waset.org/abstracts/search?q=skull%20stripper" title=" skull stripper"> skull stripper</a> </p> <a href="https://publications.waset.org/abstracts/127935/skull-extraction-for-quantification-of-brain-volume-in-magnetic-resonance-imaging-of-multiple-sclerosis-patients" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/127935.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">146</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">502</span> Extraction and Quantification of Triclosan in Wastewater Samples Using Molecularly Imprinted Membrane Adsorbent</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Siyabonga%20Aubrey%20Mhlongo">Siyabonga Aubrey Mhlongo</a>, <a href="https://publications.waset.org/abstracts/search?q=Linda%20Lunga%20Sibali"> Linda Lunga Sibali</a>, <a href="https://publications.waset.org/abstracts/search?q=Phumlane%20Selby%20Mdluli"> Phumlane Selby Mdluli</a>, <a href="https://publications.waset.org/abstracts/search?q=Peter%20Papoh%20Ndibewu"> Peter Papoh Ndibewu</a>, <a href="https://publications.waset.org/abstracts/search?q=Kholofelo%20Clifford%20Malematja"> Kholofelo Clifford Malematja</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper reports on the successful extraction and quantification of an antibacterial and antifungal agent present in some consumer products (Triclosan: C₁₂H₇Cl₃O₂)generally found in wastewater or effluents using molecularly imprinted membrane adsorbent (MIMs) followed by quantification and removal on a high-performance liquid chromatography (HPLC). Triclosan is an antibacterial and antifungal agent present in some consumer products like toothpaste, soaps, detergents, toys, and surgical cleaning treatments. The MIMs was fabricated usingpolyvinylidene fluoride (PVDF) polymer with selective micro composite particles known as molecularly imprinted polymers (MIPs)via a phase inversion by immersion precipitation technique. This resulted in an improved hydrophilicity and mechanical behaviour of the membranes. Wastewater samples were collected from the Umbogintwini Industrial Complex (UIC) (south coast of Durban, KwaZulu-Natal in South Africa). central UIC effluent treatment plant and pre-treated before analysis. Experimental parameters such as sample size, contact time, stirring speed were optimised. The resultant MIMs had an adsorption efficiency of 97% of TCS with reference to NIMs and bare membrane, which had 92%, 88%, respectively. The analytical method utilized in this review had limits of detection (LoD) and limits of quantification (LoQ) of 0.22, 0.71µgL-1 in wastewater effluent, respectively. The percentage recovery for the effluent samples was 68%. The detection of TCS was monitored for 10 consecutive days, where optimum TCS traces detected in the treated wastewater was 55.0μg/L inday 9 of the monitored days, while the lowest detected was 6.0μg/L. As the concentrations of analytefound in effluent water samples were not so diverse, this study suggested that MIMs could be the best potential adsorbent for the development and continuous progress in membrane technologyand environmental sciences, lending its capability to desalination. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=molecularly%20imprinted%20membrane" title="molecularly imprinted membrane">molecularly imprinted membrane</a>, <a href="https://publications.waset.org/abstracts/search?q=triclosan" title=" triclosan"> triclosan</a>, <a href="https://publications.waset.org/abstracts/search?q=phase%20inversion" title=" phase inversion"> phase inversion</a>, <a href="https://publications.waset.org/abstracts/search?q=wastewater" title=" wastewater"> wastewater</a> </p> <a href="https://publications.waset.org/abstracts/151834/extraction-and-quantification-of-triclosan-in-wastewater-samples-using-molecularly-imprinted-membrane-adsorbent" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/151834.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">123</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">501</span> Two-Dimensional Modeling of Spent Nuclear Fuel Using FLUENT</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Imane%20Khalil">Imane Khalil</a>, <a href="https://publications.waset.org/abstracts/search?q=Quinn%20Pratt"> Quinn Pratt</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In a nuclear reactor, an array of fuel rods containing stacked uranium dioxide pellets clad with zircalloy is the heat source for a thermodynamic cycle of energy conversion from heat to electricity. After fuel is used in a nuclear reactor, the assemblies are stored underwater in a spent nuclear fuel pool at the nuclear power plant while heat generation and radioactive decay rates decrease before it is placed in packages for dry storage or transportation. A computational model of a Boiling Water Reactor spent fuel assembly is modeled using FLUENT, the computational fluid dynamics package. Heat transfer simulations were performed on the two-dimensional 9x9 spent fuel assembly to predict the maximum cladding temperature for different input to the FLUENT model. Uncertainty quantification is used to predict the heat transfer and the maximum temperature profile inside the assembly. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=spent%20nuclear%20fuel" title="spent nuclear fuel">spent nuclear fuel</a>, <a href="https://publications.waset.org/abstracts/search?q=conduction" title=" conduction"> conduction</a>, <a href="https://publications.waset.org/abstracts/search?q=heat%20transfer" title=" heat transfer"> heat transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=uncertainty%20quantification" title=" uncertainty quantification"> uncertainty quantification</a> </p> <a href="https://publications.waset.org/abstracts/86958/two-dimensional-modeling-of-spent-nuclear-fuel-using-fluent" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86958.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">220</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">500</span> Quantification Model for Capability Evaluation of Optical-Based in-Situ Monitoring System for Laser Powder Bed Fusion (LPBF) Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Song%20Zhang">Song Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Hui%20Wang"> Hui Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Johannes%20Henrich%20Schleifenbaum"> Johannes Henrich Schleifenbaum</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Due to the increasing demand for quality assurance and reliability for additive manufacturing, the development of an advanced in-situ monitoring system is required to monitor the process anomalies as input for further process control. Optical-based monitoring systems, such as CMOS cameras and NIR cameras, are proved as effective ways to monitor the geometrical distortion and exceptional thermal distribution. Therefore, many studies and applications are focusing on the availability of the optical-based monitoring system for detecting varied types of defects. However, the capability of the monitoring setup is not quantified. In this study, a quantification model to evaluate the capability of the monitoring setups for the LPBF machine based on acquired monitoring data of a designed test artifact is presented, while the design of the relevant test artifacts is discussed. The monitoring setup is evaluated based on its hardware properties, location of the integration, and light condition. Methodology of data processing to quantify the capacity for each aspect is discussed. The minimal capability of the detectable size of the monitoring set up in the application is estimated by quantifying its resolution and accuracy. The quantification model is validated using a CCD camera-based monitoring system for LPBF machines in the laboratory with different setups. The result shows the model to quantify the monitoring system's performance, which makes the evaluation of monitoring systems with the same concept but different setups possible for the LPBF process and provides the direction to improve the setups. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=data%20processing" title="data processing">data processing</a>, <a href="https://publications.waset.org/abstracts/search?q=in-situ%20monitoring" title=" in-situ monitoring"> in-situ monitoring</a>, <a href="https://publications.waset.org/abstracts/search?q=LPBF%20process" title=" LPBF process"> LPBF process</a>, <a href="https://publications.waset.org/abstracts/search?q=optical%20system" title=" optical system"> optical system</a>, <a href="https://publications.waset.org/abstracts/search?q=quantization%20model" title=" quantization model"> quantization model</a>, <a href="https://publications.waset.org/abstracts/search?q=test%20artifact" title=" test artifact"> test artifact</a> </p> <a href="https://publications.waset.org/abstracts/139140/quantification-model-for-capability-evaluation-of-optical-based-in-situ-monitoring-system-for-laser-powder-bed-fusion-lpbf-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139140.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">197</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">499</span> Real-Time Episodic Memory Construction for Optimal Action Selection in Cognitive Robotics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Deon%20de%20Jager">Deon de Jager</a>, <a href="https://publications.waset.org/abstracts/search?q=Yahya%20Zweiri"> Yahya Zweiri</a>, <a href="https://publications.waset.org/abstracts/search?q=Dimitrios%20Makris"> Dimitrios Makris</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The three most important components in the cognitive architecture for cognitive robotics is memory representation, memory recall, and action-selection performed by the executive. In this paper, action selection, performed by the executive, is defined as a memory quantification and optimization process. The methodology describes the real-time construction of episodic memory through semantic memory optimization. The optimization is performed by set-based particle swarm optimization, using an adaptive entropy memory quantification approach for fitness evaluation. The performance of the approach is experimentally evaluated by simulation, where a UAV is tasked with the collection and delivery of a medical package. The experiments show that the UAV dynamically uses the episodic memory to autonomously control its velocity, while successfully completing its mission. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cognitive%20robotics" title="cognitive robotics">cognitive robotics</a>, <a href="https://publications.waset.org/abstracts/search?q=semantic%20memory" title=" semantic memory"> semantic memory</a>, <a href="https://publications.waset.org/abstracts/search?q=episodic%20memory" title=" episodic memory"> episodic memory</a>, <a href="https://publications.waset.org/abstracts/search?q=maximum%20entropy%20principle" title=" maximum entropy principle"> maximum entropy principle</a>, <a href="https://publications.waset.org/abstracts/search?q=particle%20swarm%20optimization" title=" particle swarm optimization"> particle swarm optimization</a> </p> <a href="https://publications.waset.org/abstracts/114710/real-time-episodic-memory-construction-for-optimal-action-selection-in-cognitive-robotics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/114710.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">156</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=quantification&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=quantification&page=3">3</a></li> <li class="page-item"><a class="page-link" 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