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elementor-col-100 elementor-top-column elementor-element elementor-element-28aaea8" data-id="28aaea8" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-155d00a elementor-widget elementor-widget-heading" data-id="155d00a" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <style>/*! elementor - v3.18.0 - 20-12-2023 */ .elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}</style><h1 class="elementor-heading-title elementor-size-default">What is QF-Pro®?</h1> </div> </div> <div class="elementor-element elementor-element-b503531 elementor-widget elementor-widget-text-editor" data-id="b503531" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <style>/*! elementor - v3.18.0 - 20-12-2023 */ .elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}</style> <p>The spatial biology technology with proven clinical value</p> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-fb49a83 elementor-section-content-middle elementor-reverse-tablet elementor-reverse-mobile elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="fb49a83" data-element_type="section" data-settings="{"background_background":"classic"}"> <div class="elementor-container elementor-column-gap-no"> <div class="elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-af7301d" data-id="af7301d" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-fe8d3a5 elementor-widget elementor-widget-image" data-id="fe8d3a5" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img decoding="async" width="800" height="131" src="https://www.hawkbiosystems.com/wp-content/uploads/2024/03/QF-Pro-RGB-Full-Colour-1024x168.png" class="attachment-large size-large wp-image-2224" alt="" srcset="https://www.hawkbiosystems.com/wp-content/uploads/2024/03/QF-Pro-RGB-Full-Colour-1024x168.png 1024w, https://www.hawkbiosystems.com/wp-content/uploads/2024/03/QF-Pro-RGB-Full-Colour-300x49.png 300w, https://www.hawkbiosystems.com/wp-content/uploads/2024/03/QF-Pro-RGB-Full-Colour-768x126.png 768w, https://www.hawkbiosystems.com/wp-content/uploads/2024/03/QF-Pro-RGB-Full-Colour-1536x252.png 1536w, https://www.hawkbiosystems.com/wp-content/uploads/2024/03/QF-Pro-RGB-Full-Colour-2048x336.png 2048w" sizes="(max-width: 800px) 100vw, 800px" /> </div> </div> <div class="elementor-element elementor-element-4e21ceb elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="4e21ceb" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <style>/*! elementor - v3.18.0 - 20-12-2023 */ .elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#0c0d0e;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon .elementor-divider-separator:before,.elementor-widget-divider--view-line_text .elementor-divider-separator:after,.elementor-widget-divider--view-line_text .elementor-divider-separator:before{display:block;content:"";border-bottom:0;flex-grow:1;border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--element-align-left .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-left .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-left .elementor-divider__element{margin-left:0}.elementor-widget-divider--element-align-right .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-right .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-right .elementor-divider__element{margin-right:0}.elementor-widget-divider:not(.elementor-widget-divider--view-line_text):not(.elementor-widget-divider--view-line_icon) .elementor-divider-separator{border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--separator-type-pattern{--divider-border-style:none}.elementor-widget-divider--separator-type-pattern.elementor-widget-divider--view-line .elementor-divider-separator,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:after,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:before,.elementor-widget-divider--separator-type-pattern:not([class*=elementor-widget-divider--view]) .elementor-divider-separator{width:100%;min-height:var(--divider-pattern-height);-webkit-mask-size:var(--divider-pattern-size) 100%;mask-size:var(--divider-pattern-size) 100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-con-inner>.elementor-widget-divider,.e-con>.elementor-widget-divider{width:var(--container-widget-width,100%);--flex-grow:var(--container-widget-flex-grow)}</style> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-fdfdb61 elementor-widget elementor-widget-text-editor" data-id="fdfdb61" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Quantifying Functions in Proteins (<strong>QF-Pro®</strong>) is a state-of-the-art bio-imaging platform able to spatially quantify functional proteomic events within fixed patient samples (FFPE, cell lines, PBMC, etc.), such as protein-protein interactions and protein post-translational modifications, at an unrivalled resolution and dynamic range. It does this by using an adaptation of Förster Resonance Energy transfer (FRET). This patented adaptation allows FRET to work reliably in patient tissue samples, with a high signal to noise ratio, for the first time.</p><p>Unlike competing technologies, such as the proximity ligation assay (PLA) or colocalisation experiments, QF-Pro® precisely measures these events at a ≤10nm resolution. Quantifying protein functions at this resolution yields mechanistic insights into signalling pathways and drug targets directly within samples.</p> </div> </div> <div class="elementor-element elementor-element-463fc80 elementor-mobile-align-center elementor-tablet-align-center elementor-widget elementor-widget-button" data-id="463fc80" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm" href="#elementor-action%3Aaction%3Dpopup%3Aopen%26settings%3DeyJpZCI6IjUzNzciLCJ0b2dnbGUiOmZhbHNlfQ%3D%3D"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">Download brochure</span> </span> </a> </div> </div> </div> </div> </div> <div class="elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-4ad74df" data-id="4ad74df" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-6c66c5b elementor-widget elementor-widget-image" data-id="6c66c5b" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img decoding="async" width="800" height="532" src="https://www.hawkbiosystems.com/wp-content/uploads/2024/04/what-is-qf-pro-1024x681.jpg" class="attachment-large size-large wp-image-3193" alt="" srcset="https://www.hawkbiosystems.com/wp-content/uploads/2024/04/what-is-qf-pro-1024x681.jpg 1024w, https://www.hawkbiosystems.com/wp-content/uploads/2024/04/what-is-qf-pro-300x200.jpg 300w, https://www.hawkbiosystems.com/wp-content/uploads/2024/04/what-is-qf-pro-768x511.jpg 768w, https://www.hawkbiosystems.com/wp-content/uploads/2024/04/what-is-qf-pro.jpg 1500w" sizes="(max-width: 800px) 100vw, 800px" /> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-a2ae6c2 elementor-section-content-middle elementor-reverse-tablet elementor-reverse-mobile elementor-section-full_width elementor-section-height-default elementor-section-height-default" data-id="a2ae6c2" data-element_type="section" data-settings="{"background_background":"classic"}"> <div class="elementor-container elementor-column-gap-no"> <div class="elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-b09d208" data-id="b09d208" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-dc9d605 elementor-widget elementor-widget-heading" data-id="dc9d605" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">QF-Pro® reagent kits</h3> </div> </div> <div class="elementor-element elementor-element-857fe3d elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="857fe3d" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-2aa2fce elementor-widget elementor-widget-text-editor" data-id="2aa2fce" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Realise the potential of QF-Pro® with our flexible reagent kits.</p> </div> </div> <div class="elementor-element elementor-element-459765d elementor-mobile-align-center elementor-tablet-align-center elementor-align-center elementor-widget elementor-widget-button" data-id="459765d" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm" href="https://www.hawkbiosystems.com/qf-pro-reagent-kits/"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">Read more</span> </span> </a> </div> </div> </div> </div> </div> <div class="elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-d2ab6b8" data-id="d2ab6b8" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-760c980 elementor-widget elementor-widget-heading" data-id="760c980" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Partnerships</h3> </div> </div> <div class="elementor-element elementor-element-8ae4185 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="8ae4185" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-b4a5229 elementor-widget elementor-widget-text-editor" data-id="b4a5229" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Discover our academic or industrial partnership opportunities</p> </div> </div> <div class="elementor-element elementor-element-4682d4f elementor-mobile-align-center elementor-tablet-align-center elementor-align-center elementor-widget elementor-widget-button" data-id="4682d4f" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm" href="https://www.hawkbiosystems.com/partnerships/"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">Read more</span> </span> </a> </div> </div> </div> </div> </div> <div class="elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-00aa52d" data-id="00aa52d" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-b20bebe elementor-widget elementor-widget-heading" data-id="b20bebe" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Violet 3.0</h3> </div> </div> <div class="elementor-element elementor-element-80338c6 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="80338c6" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-6935f0b elementor-widget elementor-widget-text-editor" data-id="6935f0b" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Simplifying FRET/FLIM to empower life science research.</p> </div> </div> <div class="elementor-element elementor-element-9d3d86e elementor-mobile-align-center elementor-tablet-align-center elementor-align-center elementor-widget elementor-widget-button" data-id="9d3d86e" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm" href="https://www.hawkbiosystems.com/violet-3/"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">Read more</span> </span> </a> </div> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-3639307 elementor-section-content-middle elementor-reverse-tablet elementor-reverse-mobile elementor-section-full_width elementor-section-height-default elementor-section-height-default" data-id="3639307" data-element_type="section" data-settings="{"background_background":"classic"}"> <div class="elementor-container elementor-column-gap-no"> <div class="elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-803babe" data-id="803babe" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-20054f2 elementor-widget elementor-widget-heading" data-id="20054f2" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Protein-Protein Interactions<br>(PPIs)</h3> </div> </div> <div class="elementor-element elementor-element-b543202 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="b543202" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-e9f8c82 elementor-widget elementor-widget-text-editor" data-id="e9f8c82" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>QF-Pro® can be used in order to quantify a range of protein-protein interactions (PPIs) within tissue samples.</p><p><span style="font-family: var( --e-global-typography-8b7e80d-font-family ), Sans-serif; font-size: var( --e-global-typography-8b7e80d-font-size ); font-weight: var( --e-global-typography-8b7e80d-font-weight ); letter-spacing: var( --e-global-typography-8b7e80d-letter-spacing ); word-spacing: var( --e-global-typography-8b7e80d-word-spacing );">This includes intracellular PPIs such as HER2/HER3 dimerisation or Akt/PDK1 interaction states. QF-Pro® can also quantify intercellular interaction states such as those of immune checkpoints, i.e., PD-1/PD-L1, CTLA-4/CD80, TIGIT/CD155.</span></p><p><span style="font-family: var( --e-global-typography-8b7e80d-font-family ), Sans-serif; font-size: var( --e-global-typography-8b7e80d-font-size ); font-weight: var( --e-global-typography-8b7e80d-font-weight ); letter-spacing: var( --e-global-typography-8b7e80d-letter-spacing ); word-spacing: var( --e-global-typography-8b7e80d-word-spacing );">These intercellular PPIs are quantified in-situ without the need for cell permeabilisation. Other interaction states that can be quantified include DNA-protein interactions.</span></p> </div> </div> </div> </div> <div class="elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-0cde4ed" data-id="0cde4ed" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-c72bfbd elementor-widget elementor-widget-heading" data-id="c72bfbd" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Post-Translational Modifications<br>(PTMs)</h3> </div> </div> <div class="elementor-element elementor-element-3c52d3e elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="3c52d3e" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-98c3e5d elementor-widget elementor-widget-text-editor" data-id="98c3e5d" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Our intracellular PTM assays quantify events such as the phosphorylation or methylation states of intracellular proteins. The spatial mapping of these PTMs allows the user to quantify the functional states (e.g. activation) of proteins within samples. PTMs that can be quantified by QF-Pro® also include glycosylation and acetylation events. These readouts are particularly useful for gaining insights on intracellular signalling pathway dynamics and designing small molecule therapeutics.</p><p>Optimised biomarkers include Akt activation state, PD1 activation state and STAT3 (Y705 or S727) activation state quantification.</p> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-426eee4 elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="426eee4" data-element_type="section" data-settings="{"background_background":"classic"}"> <div class="elementor-container elementor-column-gap-no"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3c8c5e7" data-id="3c8c5e7" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-26915eb elementor-widget elementor-widget-heading" data-id="26915eb" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">How does QF-Pro® work?</h2> </div> </div> <div class="elementor-element elementor-element-8f236f2 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="8f236f2" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-204fe4a uael-timeline--center uael-timeline-responsive-tablet uael-timeline-arrow-center elementor-widget elementor-widget-uael-timeline" data-id="204fe4a" data-element_type="widget" data-widget_type="uael-timeline.default"> <div class="elementor-widget-container"> <div class="uael-timeline-wrapper uael-timeline-node"> <div class="uael-timeline-main"> <div class="uael-days"> <div class="elementor-repeater-item-8dddf15 uael-timeline-field animate-border out-view"> <div class="uael-timeline-widget uael-timeline-right"> <div class="uael-timeline-marker"> <span class="timeline-icon-new out-view-timeline-icon"> <i aria-hidden="true" class="fas fa-circle"></i> </span> </div> <div class="uael-day-new uael-day-right"> <div class="uael-events-new"> <div class="uael-events-inner-new"> <div class="uael-content"> <div class="uael-timeline-heading-text"> <h4 class="uael-timeline-heading">QF-Pro® Assay Overview</h4> </div> <div class="uael-timeline-desc-content"><p>QF-Pro® has modified established technologies (FRET/FLIM) to create a patented assay which is able to spatially quantify protein functions in situ within FPPE tissue samples for the first time at an unprecedented resolution. This is a feat that is unachievable with existing technologies.</p></div> </div> <div class="uael-timeline-arrow"></div> </div> </div> </div> <div class="uael-timeline-date-new"> <div class="uael-date-new"><div class="inner-date-new"><div ></div></div> </div> </div> </div> </div> <div class="elementor-repeater-item-ca197ee uael-timeline-field animate-border out-view"> <div class="uael-timeline-widget uael-timeline-left"> <div class="uael-timeline-marker"> <span class="timeline-icon-new out-view-timeline-icon"> <i aria-hidden="true" class="fas fa-circle"></i> </span> </div> <div class="uael-day-new uael-day-left"> <div class="uael-events-new"> <div class="uael-events-inner-new"> <div class="uael-content"> <div class="uael-timeline-heading-text"> <h4 class="uael-timeline-heading">Utilisation of Advanced Technologies</h4> </div> <div class="uael-timeline-desc-content"><p>QF-Pro® utilises a two-site immunofluorescence-styled assay in conjunction with amplified Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM). Essentially operating as a “biochemical ruler”, QF-Pro® makes 1-10nm measurements within samples to quantify protein functional states. Our patented amplification step has allowed these readouts to perform in patient tissue samples, a feat that has been unachievable with previous FRET-based technologies.</p></div> </div> <div class="uael-timeline-arrow"></div> </div> </div> </div> <div class="uael-timeline-date-new"> <div class="uael-date-new"><div class="inner-date-new"><div ></div></div> </div> </div> </div> </div> <div class="elementor-repeater-item-dada308 uael-timeline-field animate-border out-view"> <div class="uael-timeline-widget uael-timeline-right"> <div class="uael-timeline-marker"> <span class="timeline-icon-new out-view-timeline-icon"> <i aria-hidden="true" class="fas fa-circle"></i> </span> </div> <div class="uael-day-new uael-day-right"> <div class="uael-events-new"> <div class="uael-events-inner-new"> <div class="uael-content"> <div class="uael-timeline-heading-text"> <h4 class="uael-timeline-heading">Antibody Labeling Strategy</h4> </div> <div class="uael-timeline-desc-content"><p>Two epitopes are labelled simultaneously using species-distinct antibodies. These are then detected with QF-Pro® secondary labelling reagents, one combined with a green chromophore (the donor) and the other to an amplified red chromophore (the acceptor).</p></div> </div> <div class="uael-timeline-arrow"></div> </div> </div> </div> <div class="uael-timeline-date-new"> <div class="uael-date-new"><div class="inner-date-new"><div ></div></div> </div> </div> </div> </div> <div class="elementor-repeater-item-bf97446 uael-timeline-field animate-border out-view"> <div class="uael-timeline-widget uael-timeline-left"> <div class="uael-timeline-marker"> <span class="timeline-icon-new out-view-timeline-icon"> <i aria-hidden="true" class="fas fa-circle"></i> </span> </div> <div class="uael-day-new uael-day-left"> <div class="uael-events-new"> <div class="uael-events-inner-new"> <div class="uael-content"> <div class="uael-timeline-heading-text"> <h4 class="uael-timeline-heading">Detection</h4> </div> <div class="uael-timeline-desc-content"><p>Violet 3.0 and our QF-Pro® software then automatically measures the distance between the donor and acceptor chromophore, based on the amount of energy given from the donor to the acceptor (this is FRET). This allows us to quantify the interaction or post-translational modification of a protein and spatially measure these events across a sample. Violet 3.0 makes FRET imaging easier and more accessible than ever before.</p></div> </div> <div class="uael-timeline-arrow"></div> </div> </div> </div> <div class="uael-timeline-date-new"> <div class="uael-date-new"><div class="inner-date-new"><div ></div></div> </div> </div> </div> </div> </div> <div class="uael-timeline__line"> <div class="uael-timeline__line__inner"></div> </div> </div> </div> </div> </div> </div> </div> </div> </section> <section class="elementor-section elementor-top-section elementor-element elementor-element-88dc772 elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default" data-id="88dc772" data-element_type="section" data-settings="{"background_background":"classic"}"> <div class="elementor-background-overlay"></div> <div class="elementor-container elementor-column-gap-no"> <div class="elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-d7724e7" data-id="d7724e7" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-e0ceb37 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="e0ceb37" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-d9bcc7d elementor-widget elementor-widget-heading" data-id="d9bcc7d" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <p class="elementor-heading-title elementor-size-default">Violet 3.0</p> </div> </div> <div class="elementor-element elementor-element-9c01ff4 elementor-widget elementor-widget-text-editor" data-id="9c01ff4" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Our flagship microscopy platform, Violet 3.0, simplifies FRET/FLIM to empower the use of QF-Pro<strong>®</strong>, removing all the complexity from this technology to make it accessible to researchers and clinicians of all backgrounds.</p> </div> </div> <div class="elementor-element elementor-element-ec3edcb elementor-mobile-align-center elementor-tablet-align-center elementor-widget elementor-widget-button" data-id="ec3edcb" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm" href="https://www.hawkbiosystems.com/violet-3/"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">Violet 3.0</span> </span> </a> </div> </div> </div> </div> </div> <div class="elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-946c769" data-id="946c769" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-c1d1822 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="c1d1822" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-4dff823 elementor-widget elementor-widget-heading" data-id="4dff823" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <p class="elementor-heading-title elementor-size-default">QF-Pro® Reagent Kits </p> </div> </div> <div class="elementor-element elementor-element-37a27b7 elementor-widget elementor-widget-text-editor" data-id="37a27b7" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Discover our flexible reagent kits which allow QF-Pro® to be used in any laboratory setting. Our kits contain everything you need to perform QF-Pro® analytics in house, and can be applied to any biomarker.</p> </div> </div> <div class="elementor-element elementor-element-ed17732 elementor-mobile-align-center elementor-tablet-align-center elementor-widget elementor-widget-button" data-id="ed17732" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm" href="https://www.hawkbiosystems.com/qf-pro-reagent-kits/"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">QF-Pro® Reagent Kits</span> </span> </a> </div> </div> </div> </div> </div> <div class="elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-6c484f6" data-id="6c484f6" data-element_type="column" data-settings="{"background_background":"classic"}"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-background-overlay"></div> <div class="elementor-element elementor-element-7bca95f elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="7bca95f" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-a9b1d17 elementor-widget elementor-widget-heading" data-id="a9b1d17" data-element_type="widget" data-settings="{"_animation":"none","_animation_tablet":"none"}" data-widget_type="heading.default"> <div class="elementor-widget-container"> <p class="elementor-heading-title elementor-size-default">Validated Biomarkers</p> </div> </div> <div class="elementor-element elementor-element-40e9592 elementor-widget elementor-widget-text-editor" data-id="40e9592" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Discover the wide range of QF-Pro® validated assays that we offer, ranging from immune checkpoints to kinase activation states. Our flexible technology can be applied to quantify the function of any biomarker in any disease setting.</p> </div> </div> <div class="elementor-element elementor-element-e90f5a0 elementor-mobile-align-center elementor-tablet-align-center elementor-widget elementor-widget-button" data-id="e90f5a0" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm" href="https://www.hawkbiosystems.com/validated-biomarkers/"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">Validated Biomarkers</span> </span> </a> </div> </div> </div> </div> </div> </div> </section> </div> <div data-elementor-type="footer" data-elementor-id="129" class="elementor elementor-129 elementor-location-footer" data-elementor-post-type="elementor_library"> <section class="elementor-section elementor-top-section elementor-element 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width="1em" viewBox="0 0 448 448"><title>Negative Contrast</title><path fill="currentColor" d="M416 240c-23.75-36.75-56.25-68.25-95.25-88.25 10 17 15.25 36.5 15.25 56.25 0 61.75-50.25 112-112 112s-112-50.25-112-112c0-19.75 5.25-39.25 15.25-56.25-39 20-71.5 51.5-95.25 88.25 42.75 66 111.75 112 192 112s149.25-46 192-112zM236 144c0-6.5-5.5-12-12-12-41.75 0-76 34.25-76 76 0 6.5 5.5 12 12 12s12-5.5 12-12c0-28.5 23.5-52 52-52 6.5 0 12-5.5 12-12zM448 240c0 6.25-2 12-5 17.25-46 75.75-130.25 126.75-219 126.75s-173-51.25-219-126.75c-3-5.25-5-11-5-17.25s2-12 5-17.25c46-75.5 130.25-126.75 219-126.75s173 51.25 219 126.75c3 5.25 5 11 5 17.25z"></path></svg></span><span class="pojo-a11y-toolbar-text">Negative Contrast</span> </a> </li> <li class="pojo-a11y-toolbar-item"> <a href="#" class="pojo-a11y-toolbar-link pojo-a11y-btn-background-group pojo-a11y-btn-light-background" data-action="light-background" data-action-group="schema" tabindex="-1" role="button"> <span class="pojo-a11y-toolbar-icon"><svg version="1.1" xmlns="http://www.w3.org/2000/svg" width="1em" viewBox="0 0 448 448"><title>Light Background</title><path fill="currentColor" d="M184 144c0 4.25-3.75 8-8 8s-8-3.75-8-8c0-17.25-26.75-24-40-24-4.25 0-8-3.75-8-8s3.75-8 8-8c23.25 0 56 12.25 56 40zM224 144c0-50-50.75-80-96-80s-96 30-96 80c0 16 6.5 32.75 17 45 4.75 5.5 10.25 10.75 15.25 16.5 17.75 21.25 32.75 46.25 35.25 74.5h57c2.5-28.25 17.5-53.25 35.25-74.5 5-5.75 10.5-11 15.25-16.5 10.5-12.25 17-29 17-45zM256 144c0 25.75-8.5 48-25.75 67s-40 45.75-42 72.5c7.25 4.25 11.75 12.25 11.75 20.5 0 6-2.25 11.75-6.25 16 4 4.25 6.25 10 6.25 16 0 8.25-4.25 15.75-11.25 20.25 2 3.5 3.25 7.75 3.25 11.75 0 16.25-12.75 24-27.25 24-6.5 14.5-21 24-36.75 24s-30.25-9.5-36.75-24c-14.5 0-27.25-7.75-27.25-24 0-4 1.25-8.25 3.25-11.75-7-4.5-11.25-12-11.25-20.25 0-6 2.25-11.75 6.25-16-4-4.25-6.25-10-6.25-16 0-8.25 4.5-16.25 11.75-20.5-2-26.75-24.75-53.5-42-72.5s-25.75-41.25-25.75-67c0-68 64.75-112 128-112s128 44 128 112z"></path></svg></span><span class="pojo-a11y-toolbar-text">Light Background</span> </a> </li> <li class="pojo-a11y-toolbar-item"> <a href="#" class="pojo-a11y-toolbar-link pojo-a11y-btn-links-underline" data-action="links-underline" data-action-group="toggle" tabindex="-1" role="button"> <span class="pojo-a11y-toolbar-icon"><svg version="1.1" xmlns="http://www.w3.org/2000/svg" width="1em" viewBox="0 0 448 448"><title>Links Underline</title><path fill="currentColor" d="M364 304c0-6.5-2.5-12.5-7-17l-52-52c-4.5-4.5-10.75-7-17-7-7.25 0-13 2.75-18 8 8.25 8.25 18 15.25 18 28 0 13.25-10.75 24-24 24-12.75 0-19.75-9.75-28-18-5.25 5-8.25 10.75-8.25 18.25 0 6.25 2.5 12.5 7 17l51.5 51.75c4.5 4.5 10.75 6.75 17 6.75s12.5-2.25 17-6.5l36.75-36.5c4.5-4.5 7-10.5 7-16.75zM188.25 127.75c0-6.25-2.5-12.5-7-17l-51.5-51.75c-4.5-4.5-10.75-7-17-7s-12.5 2.5-17 6.75l-36.75 36.5c-4.5 4.5-7 10.5-7 16.75 0 6.5 2.5 12.5 7 17l52 52c4.5 4.5 10.75 6.75 17 6.75 7.25 0 13-2.5 18-7.75-8.25-8.25-18-15.25-18-28 0-13.25 10.75-24 24-24 12.75 0 19.75 9.75 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