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Search results for: ELONA
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method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="ELONA"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: ELONA</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Isothermal Solid-Phase Amplification System for Detection of Yersinia pestis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Olena%20Mayboroda">Olena Mayboroda</a>, <a href="https://publications.waset.org/abstracts/search?q=Angel%20Gonzalez%20Benito"> Angel Gonzalez Benito</a>, <a href="https://publications.waset.org/abstracts/search?q=Jonathan%20Sabate%20Del%20Rio"> Jonathan Sabate Del Rio</a>, <a href="https://publications.waset.org/abstracts/search?q=Marketa%20Svobodova"> Marketa Svobodova</a>, <a href="https://publications.waset.org/abstracts/search?q=Sandra%20Julich"> Sandra Julich</a>, <a href="https://publications.waset.org/abstracts/search?q=Herbert%20Tomaso"> Herbert Tomaso</a>, <a href="https://publications.waset.org/abstracts/search?q=Ciara%20K.%20O%27Sullivan"> Ciara K. O'Sullivan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ioanis%20Katakis"> Ioanis Katakis</a> </p> <p class="card-text"><strong>Abstract:</strong></p> DNA amplification is required for most molecular diagnostic applications but conventional PCR has disadvantages for field testing. Isothermal amplification techniques are being developed to respond to this problem. One of them is the Recombinase Polymerase Amplification (RPA) that operates at isothermal conditions without sacrificing specificity and sensitivity in easy-to-use formats. In this work RPA was used for the optical detection of solid-phase amplification of the potential biowarfare agent Yersinia pestis. Thiolated forward primers were immobilized on the surface of maleimide-activated microtitre plates for the quantitative detection of synthetic and genomic DNA, with elongation occurring only in the presence of the specific template DNA and solution phase reverse primers. Quantitative detection was achieved via the use of biotinylated reverse primers and post-amplification addition of streptavidin-HRP conjugate. The overall time of amplification and detection was less than 1 hour at a constant temperature of 37oC. Single-stranded and double-stranded DNA sequences were detected achieving detection limits of 4.04*10-13 M and 3.14*10-16 M, respectively. The system demonstrated high specificity with negligible responses to non-specific targets. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=recombinase%20polymerase%20amplification" title="recombinase polymerase amplification">recombinase polymerase amplification</a>, <a href="https://publications.waset.org/abstracts/search?q=Yersinia%20pestis" title=" Yersinia pestis"> Yersinia pestis</a>, <a href="https://publications.waset.org/abstracts/search?q=solid-phase%20detection" title=" solid-phase detection"> solid-phase detection</a>, <a href="https://publications.waset.org/abstracts/search?q=ELONA" title=" ELONA"> ELONA</a> </p> <a href="https://publications.waset.org/abstracts/42960/isothermal-solid-phase-amplification-system-for-detection-of-yersinia-pestis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42960.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">303</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Legal Warranty in Real Estate Registry in Albania</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Elona%20Saliaj">Elona Saliaj</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The registration of real estate in Albania after the 90's has been a long process in time and with high cost for the country. Passing the registration system from a centralized system to a free market private system, it鈥檚 accompanied by legal uncertainties that have led to economic instability. The reforms that have been undertaken in terms of property rights have been numerous and continuous throughout the years. But despite the reforms, the system of registration of real estate, has failed to be standards requirements established by the European Union. The completion of initial registration of real estate, legal treatment of previous owners or legalization of illegal constructions remain among the main problems that prevent the development of the country in its economic sector. The performance of the registration of real estate system and dealing with issues that have appeared in the Court of First Instance, the civil section of the Albanian constitute the core of handling this analysis. This paper presents a detailed analysis on the registration system that is chosen to be applied in our country for real estate. In its content it is also determined the institution that administrates these properties, the management technique and the law that determinate its functionality. The strategy is determined for creating a modern and functional registration system and for the country remains a challenge to achieve. Identifying practical problems and providing their solutions are also the focus of reference in order to improve and modernize this important system to a state law that aims to become a member of the European Union. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=real%20estates%20registration%20system" title="real estates registration system">real estates registration system</a>, <a href="https://publications.waset.org/abstracts/search?q=comparative%20aspects" title=" comparative aspects"> comparative aspects</a>, <a href="https://publications.waset.org/abstracts/search?q=cadastral%20area" title=" cadastral area"> cadastral area</a>, <a href="https://publications.waset.org/abstracts/search?q=property%20certificate" title=" property certificate"> property certificate</a>, <a href="https://publications.waset.org/abstracts/search?q=legal%20reform" title=" legal reform"> legal reform</a> </p> <a href="https://publications.waset.org/abstracts/23609/legal-warranty-in-real-estate-registry-in-albania" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23609.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">491</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul 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