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item */ ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hs-form-booleancheckbox-display span::after { content: ""; border: 2px solid #d9d9d9; border-radius: 3px; height: 20px; left: 0px; position: absolute; top: 0px; width: 20px; background: #fff; z-index: 0; display: block; transition-property: background-color, border-color, -webkit-box-shadow; -o-transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow, -webkit-box-shadow; } /* Show when checked */ ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::after, .hs-form-booleancheckbox-display input:checked + span::after, ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::before, .hs-form-booleancheckbox-display input:checked + span::before{ opacity: 1; } .hs-form-booleancheckbox-display input:checked+label:after, .hs-form-booleancheckbox-display input:checked+label:after { background: #fff; border-color: #157479; } /* Style when focused */ ul.inputs-list.multi-container li.hs-form-checkbox label input:focus + span::after, .hs-form-booleancheckbox-display input:focus + span::after { outline: 3px solid transparent; /* For Windows high contrast mode. */ box-shadow: 0 0 .3125rem rgba(0, 0, 0, .3); border-color: #157479; } .hs_contact_country{ clear: both; } .hs-button { border-radius: 65px !important; font-size: 14px; line-height: 1.3; letter-spacing: 1.12px; font-family: "Roboto Slab", helvetica, arial, sans-serif; font-weight: 700 !important; padding: 19px 23px !important; border: 2px solid; display: inline-block; text-decoration: none; text-transform: uppercase; -webkit-appearance: none !important; -moz-appearance: none !important; appearance: none !important; } .hbspt-form textarea{max-width: 100% !important; height: 186px !important; resize: none;} .hbspt-form textarea:focus{border: 1px solid #74cbc8 !important;} .hs-fieldtype-textarea .invalid.error{border: 2px solid #E74949 !important;} .hbspt-form fieldset{max-width: 100% !important; margin-bottom: 60px;} .hbspt-form .field {margin-bottom: 27px} .hs_recaptcha{ margin-top: 23px !important; margin-bottom: 27px !important; } .hs-button, .hs-form-field input[type=text], .hs-form-field input[type=email], .hs-form-field input[type=phone], .hs-form-field input[type=number], .hs-form-field input[type=tel], .hs-form-field input[type=date], .hs-form-field textarea { appearance: none; -webkit-appearance: none; -moz-appearance: none; } .hs-default-font-element, .hs-main-font-element { font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hbspt-form .hs-button { white-space: pre-wrap } .hbspt-form .hs-richtext { word-break: break-word } .hbspt-form.submitted-message { overflow: auto } .hbspt-form fieldset.form-columns-1 .hs-input { width: 95% } .hbspt-form fieldset.form-columns-1 .input { margin-right: 0px !important } .hbspt-form fieldset.form-columns-1 input[type=checkbox], .hbspt-form fieldset.form-columns-1 input[type=radio] { width: auto } .hbspt-form fieldset.form-columns-2 .hs-form-field { width: 50%; float: left } .hbspt-form fieldset.form-columns-2 .input { margin-right: 0px !important; } .hbspt-form fieldset.form-columns-3 .hs-form-field { width: 32.7%; float: left } .hbspt-form fieldset.form-columns-3 .input { margin-right: 8px } .hbspt-form label.hs-hidden { visibility: hidden } .hbspt-form .hs-field-desc { width: 100% } .hs-custom-style .hs-input, .hs-custom-style fieldset { max-width: 100% } .hs-custom-style>div.form-columns-3 .hs-form-field, .hs-custom-style fieldset.form-columns-3 .hs-form-field { width: 33.3% } .hs-custom-style>div>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]), .hs-custom-style fieldset>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]) { width: 100%; max-width: 100% } .hs-custom-style>div input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]), .hs-custom-style fieldset input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]) { box-sizing: border-box; padding: 0 15px; min-height: 27px } .hs-custom-style>div textarea, .hs-custom-style fieldset textarea { padding: 10px 15px } .hs-custom-style .hs-dependent-field>div .hs-input:not([type=checkbox]):not([type=radio]) { width: 100% } .legal-consent-container .field.hs-form-field { margin-bottom: 8px } .legal-consent-container .hs-field-desc.checkbox-desc { margin: -12px 0 0 21px } .legal-consent-container .hs-form-booleancheckbox-display input { float: left } .legal-consent-container .hs-form-booleancheckbox-display>span { display: block !important; margin-left: 30px !important; } .legal-consent-container .hs-form-booleancheckbox-display p { margin: 0; display: inline } .legal-consent-container .hs-error-msgs label { color: #f2545b } .legal-consent-container~.hs_recaptcha { margin-top: 18px } .cookie-reset-container { font-size: 14px; margin-bottom: 10px; text-align: right } #hs-outer-captcha-target, #hs-outer-captcha-target * { display: none; height: 0; width: 0 } .hubspot-link__container { font-size: 14px; padding-bottom: 40px; position: relative; color: #9fa0a2; font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hubspot-link-text { color: #00a4bd; font-weight: 400 } .hubspot-link__container.sproket { color: #9fa0a2 } .hubspot-link { color: #9fa0a2 } .hubspot-link, .hubspot-link:hover { text-decoration: none } .hubspot-link:hover .hubspot-link-text { text-decoration: underline } .hubspot-link__icon { margin-bottom: -1px; margin-right: 5px } .hubspot-link__container.sproket .hubspot-link__icon { width: 30px; margin-right: 0; float: left; margin-top: -9px; margin-left: -5px } .hubspot_signup_form .hs_email{margin-bottom: 0px !important; width: 80% !important; float: left !important;} .hubspot_signup_form .hs_submit{width: 20% !important; float: left !important; } .hubspot_signup_form .hs-button{padding: 12.5px 15px !important;} .hubspot_signup_form .hs_email label{display: none !important;} .hubspot_signup_form ul.no-list{position: absolute !important; top: 48px !important;} .hubspot_signup_form {position: relative !important;} .hubspot_signup_form .submitted-message{color: #ab8940 !important; font-size: 22px !important; } .hubspot_white .hbspt-form label, .hubspot_white .hs-form-required { color: #fff; } .hubspot_white .hs-input, .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hubspot_white .hs-form-booleancheckbox-display span::before{ background-color: #fff !important; } .hubspot_white .hs_submit { text-align: center; } .hubspot_white .hs-form select.is-placeholder { opacity: 1; } .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hubspot_white .hs-form-booleancheckbox-display span::after { border-color: #3f4852; } input[type=submit].hs-button.primary.large{ color: #fff; background: #00181e; border-color: #00181e !important; } input[type=submit].hs-button.primary.large:hover { background: #74cbc8 !important; border-color: #74cbc8 !important; } /***/ .hbspt-form .hs-input[type=checkbox]{ cursor: pointer; } .hs-form-booleancheckbox-display input:checked + span::after{ background: #fff; border-color: #157479; } .hbspt-form .form-columns-2 { display: flex; flex-flow: row wrap; -webkit-box-direction: normal; } .hbspt-form .hs_firstname { flex: 1; -webkit-box-flex: 1; } .hbspt-form .hs_submit{ margin-top: 60px; } .newsletter-form .hs_submit{margin-top: 45px;} .newsletter-form form .gfield_consent_description { border: none !important; } /*New Landing page*/ .landing-middle-section{ padding-right: 0px !important; } .left-col{max-width:93%} .left-col-para{padding-right:30px;} .left-col video{border-radius:4px;} /* Kit asssesment */ .kits_form_col .hbspt-form .hs-input[type=checkbox] { height: 20px !important; width: 20px !important; } .kits_form_col {padding-bottom: 45px;} .hs-richtext h4 {margin-bottom: 15px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 15px;} .kits_form_col .hbspt-form li.hs-form-checkbox label span{ margin-left: 0px !important; padding-left: 31px; } .hbspt-form label.hs-form-radio-display { position: relative; padding: 4px 31px; } .hbspt-form .hs-form-radio-display span, .hbspt-form .hs-form-checkbox-display span { position: relative; cursor: pointer; font-size: 15px; font-weight: 300; } .hbspt-form .hs-input[type=radio] { position: absolute; left: 0; z-index: 2; width: 20px !important; height: 20px !important; outline: none; cursor: pointer; background-color: transparent !important; border-radius: 50%; } .hbspt-form .hs_protocol_synopsis_upload .input{position: relative;} .hbspt-form input[type="file"]::-webkit-file-upload-button { cursor: pointer; background-color: #157479; padding: 10px 20px !important; border: 1px solid #157479; border-top-left-radius: 0px; border-bottom-left-radius: 0px; border-top-right-radius: 6px; border-bottom-right-radius: 6px; color: #fff; position: absolute; top: 0; bottom: 0; right: 0; } .hbspt-form input[type="file"] { cursor: pointer; padding: 10px 20px !important; position: relative; top: 0; bottom: 0; right: 0; } /*Form Responsive CSS*/ @media only screen and (min-width: 1024px) and (max-width: 1366px){ .hs_email, .hs_contact_country { clear: both; } } @media only screen and (min-width: 992px) and (max-width: 1024px){ .hs_email, .hs_contact_country { clear: both; } } @media (min-width: 640px) and (max-width: 1120px) { .hbspt-form h1{font-size: 36px !important;} } @media screen and (min-width: 1120px) { .hbspt-form h1{font-size: 45px !important;margin-bottom: 17px !important;} .hbspt-form .gform_heading p{font-size: 17px !important;} } @media only screen and (min-width: 768px) and (max-width: 992px){ .hs_email, .hs_contact_country{ clear: both; } } @media (min-width: 767px) and (max-width: 1024px) { body .hubspot_signup_form .hs_email{width: 66% !important; float: left !important;} } @media screen and (max-width: 992px){ .assay-kit-hbspt-form .fl-col { width: 100%; } } @media screen and (max-width:812px){ .assay-kit-hbspt-form .fl-col { max-width: 100%; } .hbspt-form .hs-fieldtype-select .input:before{ right: 20px !important; } } @media screen and (max-width: 767px){ .hs_submit .actions{padding-top: 0px !important; margin-top: 0px !important;} body .hbspt-form fieldset.form-columns-2 .input {margin-right: 0px !important;} body fieldset.form-columns-3 .input{margin-right: 0px !important;} .hs-form-field .hs-input { width: 100% !important; } .hs-fieldtype-textarea .input{margin-right: 0px !important;} .hs_firstname, .hs_phone, .hs_development_area{ width: 100% !important; } } @media screen and (min-width: 640px){ .hbspt-form .hs_firstname, .kits_form_col .hs_email{ 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href="https://www.bioagilytix.com/speak-to-scientist/">Speak to a Scientist</a></div></div></div><div class="breadcrumbs"><span><span><a href="https://www.bioagilytix.com/"><i class="home-icon"></i><span class="screen-reader-text">Home</span></a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/">Techniques & Platforms</a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/instrumentation/">Instrumentation</a></span> <span class="breadcrumb-separator"></span> <span class="breadcrumb_last" aria-current="page">Luminex</span></span></div></div></section><div class="wp-block-group alignwide is-layout-constrained wp-container-core-group-is-layout-1 wp-block-group-is-layout-constrained"><p class="has-text-align-center has-large-font-size">BioAgilytix uses Luminex’s innovative bead-based technology to perform many immunoassays under nonregulated and GxP conditions. We operate Luminex FLEXMAP 3D<sup>®</sup> systems at multiple locations globally with xPonent software that is 21 CFR Part 11 compliant for our regulated assay support. We maintain several data analysis software options as well to provide versatility during the drug development process.  </p></div><div id="block_2e5b4a0f381e895b3406bc76713abc4b" class="wp-block em-media-content em-mt--none em-mb--none alignfull"><div class="em-media-content__inner em-media-content__inner--media-right has-gray-xxl-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--right"> <img fetchpriority="high" decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/flexmap-3d-1380x776.jpg" alt="flexmap-3d" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/flexmap-3d-1380x776.jpg 1380w, https://www.bioagilytix.com/wp-content/uploads/2023/08/flexmap-3d-1120x630.jpg 1120w, https://www.bioagilytix.com/wp-content/uploads/2023/08/flexmap-3d-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/flexmap-3d-640x360.jpg 640w" width="1380" height="776"></div><div class="em-media-content__content em-media-content__content--left em-media-content__content--add-padding"><h2 class="h2"><strong>Accuracy and reproducibility for multiplexing needs </strong></h2><p class="">BioAgilytix employs Luminex FLEXMAP 3D systems to run bead-based immunoassays. This platform give us the ability to multiplex many different assays simultaneously, providing greater information from a lower sample volume and in a shorter timeframe than required for traditional immunoassay methods. </p></div></div></div><div id="block_1542bb4c414e2b5e1d791a5cce520699" class="wp-block em-media-content em-mt--lg em-mb--lg alignwide"><div class="em-media-content__inner em-media-content__inner--media-left has-none-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--left"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083.jpg" alt="Researcher writing notes" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083-300x200.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083-768x512.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083-100x67.jpg 100w" width="1000" height="667"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%201000%20667%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083.jpg" alt="Researcher writing notes" class="lazyload object-fit--cover" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083-300x200.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083-768x512.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_2138321083-100x67.jpg 100w" width="1000" height="667"></div><div class="em-media-content__content em-media-content__content--right em-media-content__content--add-padding"><h6 class="has-primary-l-color has-text-color h6"><strong><strong>Ideal Luminex Applications: </strong></strong></h6><h2 class="h2"><strong><strong>For simultaneously high plex and throughput </strong></strong></h2><p class="">Luminex is ideal for bioanalytical applications that require both high plex and high throughput at the same time. It can yield significantly more information from limited-volume samples. While traditional ELISA and other technologies can also excel at high throughput applications, they are often limited in the total number of tests that can be plexed together into a single assay. </p><p class="">As a flow-based system, our Luminex platforms read and quantitate bead events individually, and can evaluate many markers on each sample. For example, a 30-plex kit can obtain up to 3,000 data points a day for 100 samples. BioAgilytix most frequently uses Luminex in our work with biomarkers. Our team has optimized and validated several cytokine, chemokine, growth factor, and biomarker assays ranging from known vendor protein panels (i.e., pro- or anti-inflammatory panels) to completely customized panels. We can also perform nonregulated analysis of antibodies to specific HLA haplotypes expressed by allogeneic cell therapy using Luminex. </p></div></div></div><div class="wp-block-emagine-background alignfull has-quaternary-background-color has-background-dim em-pt--xl em-pb--xl em-mt--lg em-mb--md"><div class="wp-block-emagine-background__content"><div class="wp-block-emagine-background__content-inner"><div class="wp-block-emagine-grid alignwide wp-block-emagine-grid--left-heavy"><div class="wp-block-emagine-grid__inner em-grid-equalHeight-left-alternateRows"><div class="wp-block-emagine-column em-col-8_sm-12"><h6 class="has-primary-l-color has-text-color h6"><strong><strong><strong>Key Luminex Benefits: </strong></strong></strong></h6><h2 class="has-em-white-color has-text-color h2"><strong><strong><strong>Multiplexing and high speed and throughput </strong></strong></strong></h2><p class="">The multiplexing functionality Luminex enables reduces time, labor, and costs over traditional methods such as ELISA or Western blot. It is particularly advantageous for small sampling volumes. The dual laser-based reader delivers robust data from limited events. Throughput is also maximized, with multiple simultaneous analytes being detected in a single sample. </p><p class="">Luminex also offers strong versatility in that the single xMAP technology-based system performs a multitude of assay types, from nucleic acids and antigen-antibody binding, to enzyme, receptor-ligand, and other protein interactions.  </p></div><div class="wp-block-emagine-column em-col-4_sm-12"><p class=""><strong>Top benefits of Luminex Bio-Plex:</strong> </p><ul class="wp-block-list"><li class="">Multiplexing </li><li class="">High speed and throughput </li><li class="">Small sample volumes </li><li class="">Versatility and flexibility </li><li class="">High accuracy </li><li class="">Reproducibility </li></ul></div></div></div></div></div></div><div id="block_a0825059da42489b36c23b2d0a3b708f" class="wp-block em-media-content em-mt--lg em-mb--sm alignwide"><div class="em-media-content__inner em-media-content__inner--media-left has-none-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--left"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1.jpg" alt="Technician in the lab" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1-300x200.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1-768x512.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1-100x67.jpg 100w" width="1000" height="667"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%201000%20667%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1.jpg" alt="Technician in the lab" class="lazyload object-fit--cover" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1-300x200.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1-768x512.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1274923345-1-100x67.jpg 100w" width="1000" height="667"></div><div class="em-media-content__content em-media-content__content--right em-media-content__content--add-padding"><h3 class="h3"><strong><strong><strong><strong>How the Luminex platforms work </strong></strong></strong></strong></h3><p class="">Based on the principles of flow cytometry, Luminex FLEXMAP 3D analyzer combines advanced fluidics, optics, and digital signal processing with Luminex’s proprietary technology to perform discrete assays on the surface of color-coded beads known as ‘microspheres.’ </p><p class="">Luminex platforms are built around three core technologies: the fluorescently dyed microspheres, or beads, provided from Luminex; a flow cytometer with two lasers and associated optics; and a high-speed digital processor able to efficiently manage digital output. The analyzer employs fluorescent methods for detection, using the bead sets to identify the analytes, and detection antibodies to measure the quantity of analyte. The use of different colored beads allows for simultaneous detection of many analytes in the same sample, and laser excitation is employed to determine analyte concentration. </p><p class="">The main advantages of the Luminex FLEXMAP 3D system are the ability to multiplex up to 500 analytes with minimal sample volume and automation to support high-throughput bioassay applications. </p></div></div></div></article></main><footer class="site-footer site-footer--has-logo site-footer--align-brand-left"><div class="deep-footer clearfix"><div class="alignwide"><div class="em-grid"><div class="em-col-6_md-12"><div class="deep-footer__brand"><div class="deep-footer__logo" style="max-width: 300px"> <a href="https://www.bioagilytix.com"><div class="em-image"><div class="em-image__ratio" style="padding-top:21.420572708367%;"> <svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 187.53 40.17" width="187.53px" height="40.17px" class=""><g fill="none"><g fill="#fff"><path d="M12.04 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