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vector-pinnable-header-pinned" data-feature-name="appearance-pinned" data-pinnable-element-id="vector-appearance" data-pinned-container-id="vector-appearance-pinned-container" data-unpinned-container-id="vector-appearance-unpinned-container" > <div class="vector-pinnable-header-label">外观</div> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-pin-button" data-event-name="pinnable-header.vector-appearance.pin">移至侧栏</button> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-unpin-button" data-event-name="pinnable-header.vector-appearance.unpin">隐藏</button> </div> </div> </div> </nav> </div> </div> <div id="bodyContent" class="vector-body" aria-labelledby="firstHeading" data-mw-ve-target-container> <div class="vector-body-before-content"> <div class="mw-indicators"> </div> <div id="siteSub" class="noprint">维基百科，自由的百科全书</div> </div> <div id="contentSub"><div id="mw-content-subtitle"></div></div> <div id="mw-content-text" class="mw-body-content"><div class="mw-content-ltr mw-parser-output" lang="zh" dir="ltr"><p><b>NdeI</b>是一種发现於<a href="/wiki/%E7%B4%B0%E8%8F%8C" class="mw-redirect" title="細菌">細菌</a>脱氮奈瑟菌（<i>Neisseria denitrificans</i>）的II型<a href="/wiki/%E9%99%90%E5%88%B6%E9%85%B6" title="限制酶">限制酶</a>，其<a href="/wiki/%E8%AF%86%E5%88%AB%E5%BA%8F%E5%88%97" title="识别序列">识别序列</a>为<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>： </p> <pre>5'CATATG 3'GTATAC </pre> <p>切割产生的<a href="/wiki/%E7%B2%98%E6%80%A7%E6%9C%AB%E7%AB%AF" class="mw-redirect" title="粘性末端">粘性末端</a>为 </p> <pre>5'---CA TATG---3' 3'---GTAT AC---5' </pre> <p><br /> </p> <div class="mw-heading mw-heading2"><h2 id="參考文獻"><span id=".E5.8F.83.E8.80.83.E6.96.87.E7.8D.BB"></span>參考文獻</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=NdeI&action=edit&section=1" title="编辑章节：參考文獻"><span>编辑</span></a><span class="mw-editsection-bracket">]</span></span></div> <div class="reflist columns references-column-count references-column-count-2" style="-moz-column-count: 2; -webkit-column-count: 2; column-count: 2; list-style-type: decimal;"> <ol class="references"> <li id="cite_note-1"><span class="mw-cite-backlink"><b><a href="#cite_ref-1">^</a></b></span> <span class="reference-text"><cite class="citation journal">Watson RJ, Schildraut I, Qiang BQ, Martin SM, Visentin LP. NdeI: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5'-CATATG-3' sequences. FEBS Lett. Dec 13, 1982, <b>150</b> (1): 114–6. <a rel="nofollow" class="external text" href="//www.ncbi.nlm.nih.gov/pubmed/6297965"><span title="公共医学识别码">PMID 6297965</span></a>. <a rel="nofollow" class="external text" href="https://api.semanticscholar.org/CorpusID:30803809"><span title="Semantic Scholar">S2CID 30803809</span></a>. <a rel="nofollow" class="external text" href="https://doi.org/10.1016%2F0014-5793%2882%2981315-x"><span title="數位物件識別號">doi:10.1016/0014-5793(82)81315-x</span></a>.</cite><span title="ctx_ver=Z39.88-2004&rfr_id=info%3Asid%2Fzh.wikipedia.org%3ANdeI&rft.atitle=NdeI%3A+a+restriction+endonuclease+from+Neisseria+denitrificans+which+cleaves+DNA+at+5%27-CATATG-3%27+sequences&rft.au=Martin%2C+SM&rft.au=Qiang%2C+BQ&rft.au=Schildraut%2C+I&rft.au=Visentin%2C+LP&rft.aufirst=RJ&rft.aulast=Watson&rft.date=1982-12-13&rft.genre=article&rft.issue=1&rft.jtitle=FEBS+Lett.&rft.pages=114-6&rft.volume=150&rft_id=https%3A%2F%2Fapi.semanticscholar.org%2FCorpusID%3A30803809&rft_id=info%3Adoi%2F10.1016%2F0014-5793%2882%2981315-x&rft_id=info%3Apmid%2F6297965&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal" class="Z3988"><span style="display:none;"> </span></span></span> </li> </ol></div> <div class="navbox-styles"><style data-mw-deduplicate="TemplateStyles:r84265675">.mw-parser-output .hlist dl,.mw-parser-output .hlist ol,.mw-parser-output .hlist ul{margin:0;padding:0}.mw-parser-output .hlist dd,.mw-parser-output .hlist dt,.mw-parser-output .hlist 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data-mw-deduplicate="TemplateStyles:r84261037">.mw-parser-output .navbox{box-sizing:border-box;border:1px solid #a2a9b1;width:100%;clear:both;font-size:88%;text-align:center;padding:1px;margin:1em auto 0}.mw-parser-output .navbox .navbox{margin-top:0}.mw-parser-output .navbox+.navbox,.mw-parser-output .navbox+.navbox-styles+.navbox{margin-top:-1px}.mw-parser-output .navbox-inner,.mw-parser-output .navbox-subgroup{width:100%}.mw-parser-output .navbox-group,.mw-parser-output .navbox-title,.mw-parser-output .navbox-abovebelow{text-align:center;padding-left:1em;padding-right:1em}.mw-parser-output .navbox-group{white-space:nowrap;text-align:right}.mw-parser-output .navbox,.mw-parser-output .navbox-subgroup{background-color:#fdfdfd}.mw-parser-output .navbox-list{border-color:#fdfdfd}.mw-parser-output .navbox-list-with-group{text-align:left;border-left-width:2px;border-left-style:solid}.mw-parser-output tr+tr>.navbox-abovebelow,.mw-parser-output tr+tr>.navbox-group,.mw-parser-output tr+tr>.navbox-image,.mw-parser-output tr+tr>.navbox-list{border-top:2px solid #fdfdfd}.mw-parser-output .navbox-title{background-color:#ccf;position:relative}.mw-parser-output .navbox-abovebelow,.mw-parser-output .navbox-group,.mw-parser-output .navbox-subgroup .navbox-title{background-color:#ddf}.mw-parser-output .navbox-subgroup .navbox-group,.mw-parser-output .navbox-subgroup .navbox-abovebelow{background-color:#e6e6ff}.mw-parser-output .navbox-even{background-color:#f7f7f7}.mw-parser-output .navbox-odd{background-color:transparent}.mw-parser-output .navbox .hlist td dl,.mw-parser-output .navbox .hlist td ol,.mw-parser-output .navbox .hlist td ul,.mw-parser-output .navbox td.hlist dl,.mw-parser-output .navbox td.hlist ol,.mw-parser-output .navbox td.hlist ul{padding:0.125em 0}.mw-parser-output .navbox .navbar{display:block;font-size:100%}.mw-parser-output .navbox-title .navbar{float:left;text-align:left;margin-right:0.5em;width:auto;padding-left:0.2em;position:absolute;left:1em}.mw-parser-output .navbox .mw-collapsible-toggle{margin-left:0.5em;position:absolute;right:1em}body.skin--responsive .mw-parser-output .navbox-image img{max-width:none!important}@media print{body.ns-0 .mw-parser-output .navbox{display:none!important}}</style></div><div role="navigation" class="navbox" aria-labelledby="限制修飾系統：限制酶" style="padding:3px"><table class="nowraplinks mw-collapsible autocollapse navbox-inner" style="border-spacing:0;background:transparent;color:inherit"><tbody><tr><th scope="col" class="collapsible-title navbox-title" colspan="2"><link rel="mw-deduplicated-inline-style" href="mw-data:TemplateStyles:r84265675"><style data-mw-deduplicate="TemplateStyles:r84244141">.mw-parser-output .navbar{display:inline;font-weight:normal}.mw-parser-output .navbar-collapse{float:left;text-align:left}.mw-parser-output .navbar-boxtext{word-spacing:0}.mw-parser-output .navbar ul{display:inline-block;white-space:nowrap;line-height:inherit}.mw-parser-output .navbar-brackets::before{margin-right:-0.125em;content:"[ "}.mw-parser-output .navbar-brackets::after{margin-left:-0.125em;content:" ]"}.mw-parser-output .navbar li{word-spacing:-0.125em}.mw-parser-output .navbar a>span,.mw-parser-output .navbar a>abbr{text-decoration:inherit}.mw-parser-output .navbar-mini abbr{font-variant:small-caps;border-bottom:none;text-decoration:none;cursor:inherit}.mw-parser-output .navbar-ct-full{font-size:110%;margin:0 8em}.mw-parser-output .navbar-ct-mini{font-size:110%;margin:0 5em}html.skin-theme-clientpref-night .mw-parser-output .navbar li a abbr{color:var(--color-base)!important}@media(prefers-color-scheme:dark){html.skin-theme-clientpref-os .mw-parser-output .navbar li a abbr{color:var(--color-base)!important}}@media print{.mw-parser-output .navbar{display:none!important}}</style><div class="navbar plainlinks hlist navbar-mini"><ul><li class="nv-view"><a href="/wiki/Template:%E9%99%90%E5%88%B6%E9%85%B6" title="Template:限制酶"><abbr title="查看该模板">查</abbr></a></li><li class="nv-talk"><a href="/w/index.php?title=Template_talk:%E9%99%90%E5%88%B6%E9%85%B6&action=edit&redlink=1" class="new" title="Template talk:限制酶（页面不存在）"><abbr title="讨论该模板">论</abbr></a></li><li class="nv-edit"><a href="/wiki/Special:%E7%BC%96%E8%BE%91%E9%A1%B5%E9%9D%A2/Template:%E9%99%90%E5%88%B6%E9%85%B6" title="Special:编辑页面/Template:限制酶"><abbr title="编辑该模板">编</abbr></a></li></ul></div><div id="限制修飾系統：限制酶" style="font-size:110%;margin:0 5em"><a href="/wiki/%E9%99%90%E5%88%B6%E4%BF%AE%E9%A3%BE%E7%B3%BB%E7%B5%B1" title="限制修飾系統">限制修飾系統</a>：<a href="/wiki/%E9%99%90%E5%88%B6%E9%85%B6" title="限制酶">限制酶</a></div></th></tr><tr><th scope="row" class="navbox-group" style="width:1%">基本概念</th><td class="navbox-list-with-group navbox-list navbox-odd hlist" style="width:100%;padding:0px"><div style="padding:0em 0.25em"> <ul><li><a href="/wiki/%E5%90%8C%E8%A3%82%E9%85%B6" title="同裂酶">同裂酶</a></li> <li><a href="/wiki/%E5%90%8C%E5%B0%BE%E9%85%B6" title="同尾酶">同尾酶</a></li> <li><a href="/wiki/%E5%BC%82%E8%A3%82%E9%85%B6" title="异裂酶">异裂酶</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/%E8%AF%86%E5%88%AB%E5%BA%8F%E5%88%97" title="识别序列">识别序列</a>为4bp</th><td class="navbox-list-with-group navbox-list navbox-even hlist" style="width:100%;padding:0px"><div style="padding:0em 0.25em"> <ul><li><a href="/wiki/TaqI" title="TaqI"><i>Taq</i>I</a></li> <li><a href="/w/index.php?title=Sau3A&action=edit&redlink=1" class="new" title="Sau3A（页面不存在）"><i>Sau</i>3A</a></li> <li><a href="/w/index.php?title=AluI&action=edit&redlink=1" class="new" title="AluI（页面不存在）"><i>Alu</i>I*</a></li> <li><a href="/w/index.php?title=HaeIII&action=edit&redlink=1" class="new" title="HaeIII（页面不存在）"><i>Hae</i>III*</a></li> <li><a href="/wiki/DpnII" title="DpnII"><i>Dpn</i>II</a></li> <li><a href="/wiki/NlaIII" title="NlaIII"><i>Nla</i>III</a></li> <li><a href="/wiki/HpaII" title="HpaII"><i>Hpa</i>II</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/%E8%AF%86%E5%88%AB%E5%BA%8F%E5%88%97" title="识别序列">识别序列</a>为5bp</th><td class="navbox-list-with-group navbox-list navbox-odd hlist" style="width:100%;padding:0px"><div style="padding:0em 0.25em"> <ul><li><a href="/w/index.php?title=EcoRII&action=edit&redlink=1" class="new" title="EcoRII（页面不存在）"><i>Eco</i>RII</a></li> <li><a href="/wiki/FokI" title="FokI"><i>Fok</i>I</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/%E8%AF%86%E5%88%AB%E5%BA%8F%E5%88%97" title="识别序列">识别序列</a>为6bp</th><td class="navbox-list-with-group navbox-list navbox-even hlist" style="width:100%;padding:0px"><div style="padding:0em 0.25em"> <ul><li><a href="/wiki/AaaI" title="AaaI"><i>Aaa</i>I</a></li> <li><a href="/wiki/AvaI" title="AvaI"><i>Ava</i>I</a></li> <li><a href="/w/index.php?title=BglII&action=edit&redlink=1" class="new" title="BglII（页面不存在）"><i>Bgl</i>II</a></li> <li><a href="/w/index.php?title=PovII&action=edit&redlink=1" class="new" title="PovII（页面不存在）"><i>Pov</i>II*</a></li> <li><a href="/wiki/EcoRV" title="EcoRV"><i>Eco</i>RV*</a></li> <li><a href="/wiki/EcoRI" title="EcoRI"><i>Eco</i>RI</a></li> <li><a href="/wiki/BamHI" title="BamHI"><i>Bam</i>HI</a></li> <li><a href="/wiki/HindIII" title="HindIII"><i>Hind</i>III</a></li> <li><a href="/wiki/XbaI" title="XbaI"><i>Xba</i>I</a></li> <li><a href="/wiki/XhoI" title="XhoI"><i>Xho</i>I</a></li> <li><a href="/w/index.php?title=SacI&action=edit&redlink=1" class="new" title="SacI（页面不存在）"><i>Sac</i>I</a></li> <li><a href="/wiki/PstI" title="PstI"><i>Pst</i>I</a></li> <li><a class="mw-selflink selflink"><i>Nde</i>I</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/%E8%AF%86%E5%88%AB%E5%BA%8F%E5%88%97" title="识别序列">识别序列</a>为8bp</th><td class="navbox-list-with-group navbox-list navbox-odd hlist" style="width:100%;padding:0px"><div style="padding:0em 0.25em"> <ul><li><a href="/w/index.php?title=NotI&action=edit&redlink=1" class="new" title="NotI（页面不存在）"><i>Not</i>I</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites（页面不存在）">列表</a></th><td class="navbox-list-with-group navbox-list navbox-even hlist" style="width:100%;padding:0px"><div style="padding:0em 0.25em"> <ul><li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_A&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: A（页面不存在）">A</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_Ba%E2%80%93Bc&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: Ba–Bc（页面不存在）">Ba–Bc</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_Bd%E2%80%93Bp&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: Bd–Bp（页面不存在）">Bd–Bp</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_Bsa%E2%80%93Bso&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: Bsa–Bso（页面不存在）">Bsa–Bso</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_Bsp%E2%80%93Bss&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: Bsp–Bss（页面不存在）">Bsp–Bss</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_Bst%E2%80%93Bv&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: Bst–Bv（页面不存在）">Bst–Bv</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_C-D&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: C-D（页面不存在）">C-D</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_E-F&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: E-F（页面不存在）">E-F</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_G-K&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: G-K（页面不存在）">G-K</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_L-N&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: L-N（页面不存在）">L-N</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_O-R&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: O-R（页面不存在）">O-R</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_S&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: S（页面不存在）">S</a></li> <li><a href="/w/index.php?title=List_of_restriction_enzyme_cutting_sites:_T-Z&action=edit&redlink=1" class="new" title="List of restriction enzyme cutting sites: T-Z（页面不存在）">T-Z</a></li></ul> </div></td></tr><tr><td class="navbox-abovebelow" colspan="2" style="padding:0;"><div>*表示切割产物为<a href="/wiki/%E5%B9%B3%E6%BB%91%E6%9C%AB%E7%AB%AF" class="mw-redirect" title="平滑末端">平末端</a></div></td></tr></tbody></table></div>    </div><noscript><img src="https://login.wikimedia.org/wiki/Special:CentralAutoLogin/start?type=1x1" alt="" width="1" height="1" style="border: none; position: absolute;"></noscript> <div class="printfooter" data-nosnippet="">检索自“<a dir="ltr" href="https://zh.wikipedia.org/w/index.php?title=NdeI&oldid=68022906">https://zh.wikipedia.org/w/index.php?title=NdeI&oldid=68022906</a>”</div></div> <div id="catlinks" class="catlinks" data-mw="interface"><div id="mw-normal-catlinks" class="mw-normal-catlinks"><a href="/wiki/Special:%E9%A1%B5%E9%9D%A2%E5%88%86%E7%B1%BB" 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