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Search results for: Harunori Kawabe
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text-center" style="font-size:1.6rem;">Search results for: Harunori Kawabe</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> A Nucleic Acid Extraction Method for High-Viscosity Floricultural Samples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Harunori%20Kawabe">Harunori Kawabe</a>, <a href="https://publications.waset.org/abstracts/search?q=Hideyuki%20Aoshima"> Hideyuki Aoshima</a>, <a href="https://publications.waset.org/abstracts/search?q=Koji%20Murakami"> Koji Murakami</a>, <a href="https://publications.waset.org/abstracts/search?q=Minoru%20Kawakami"> Minoru Kawakami</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuka%20Nakano"> Yuka Nakano</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20D.%20Ordinario"> David D. Ordinario</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20W.%20Crawford"> C. W. Crawford</a>, <a href="https://publications.waset.org/abstracts/search?q=Iri%20Sato-Baran"> Iri Sato-Baran</a> </p> <p class="card-text"><strong>Abstract:</strong></p> With the recent advances in gene editing technologies allowing the rewriting of genetic sequences, additional market growth in the global floriculture market beyond previous trends is anticipated through increasingly sophisticated plant breeding techniques. As a prerequisite for gene editing, the gene sequence of the target plant must first be identified. This necessitates the genetic analysis of plants with unknown gene sequences, the extraction of RNA, and comprehensive expression analysis. Consequently, a technology capable of consistently and effectively extracting high-purity DNA and RNA from plants is of paramount importance. Although model plants, such as Arabidopsis and tobacco, have established methods for DNA and RNA extraction, floricultural species such as roses present unique challenges. Different techniques to extract DNA and RNA from various floricultural species were investigated. Upon sampling and grinding the petals of several floricultural species, it was observed that nucleic acid extraction from the ground petal solutions of low viscosity was straightforward; solutions of high viscosity presented a significant challenge. It is postulated that the presence of substantial quantities of polysaccharides and polyphenols in the plant tissue was responsible for the inhibition of nucleic acid extraction. Consequently, attempts were made to extract high-purity DNA and RNA by improving the CTAB method and combining it with commercially available nucleic acid extraction kits. The quality of the total extracted DNA and RNA was evaluated using standard methods. Finally, the effectiveness of the extraction method was assessed by determining whether it was possible to create a library that could be applied as a suitable template for a next-generation sequencer. In conclusion, a method was developed for consistent and accurate nucleic acid extraction from high-viscosity floricultural samples. These results demonstrate improved techniques for DNA and RNA extraction from flowers, help facilitate gene editing of floricultural species and expand the boundaries of research and commercial opportunities. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=floriculture" title="floriculture">floriculture</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20editing" title=" gene editing"> gene editing</a>, <a href="https://publications.waset.org/abstracts/search?q=next-generation%20sequencing" title=" next-generation sequencing"> next-generation sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=nucleic%20acid%20extraction" title=" nucleic acid extraction"> nucleic acid extraction</a> </p> <a href="https://publications.waset.org/abstracts/191049/a-nucleic-acid-extraction-method-for-high-viscosity-floricultural-samples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/191049.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">29</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> MP-SMC-I Method for Slip Suppression of Electric Vehicles under Braking</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tohru%20Kawabe">Tohru Kawabe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, a new SMC (Sliding Mode Control) method with MP (Model Predictive Control) integral action for the slip suppression of EV (Electric Vehicle) under braking is proposed. The proposed method introduce the integral term with standard SMC gain , where the integral gain is optimized for each control period by the MPC algorithms. The aim of this method is to improve the safety and the stability of EVs under braking by controlling the wheel slip ratio. There also include numerical simulation results to demonstrate the effectiveness of the method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sliding%20mode%20control" title="sliding mode control">sliding mode control</a>, <a href="https://publications.waset.org/abstracts/search?q=model%20predictive%20control" title=" model predictive control"> model predictive control</a>, <a href="https://publications.waset.org/abstracts/search?q=integral%20action" title=" integral action"> integral action</a>, <a href="https://publications.waset.org/abstracts/search?q=electric%20vehicle" title=" electric vehicle"> electric vehicle</a>, <a href="https://publications.waset.org/abstracts/search?q=slip%20suppression" title=" slip suppression"> slip suppression</a> </p> <a href="https://publications.waset.org/abstracts/28617/mp-smc-i-method-for-slip-suppression-of-electric-vehicles-under-braking" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28617.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">561</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Improvement of Ride Comfort of Turning Electric Vehicle Using Optimal Speed Control </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yingyi%20Zhou">Yingyi Zhou</a>, <a href="https://publications.waset.org/abstracts/search?q=Tohru%20Kawabe"> Tohru Kawabe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> With the spread of EVs (electric Vehicles), the ride comfort has been gaining a lot of attention. The influence of the lateral acceleration is important for the improvement of ride comfort of EVs as well as the longitudinal acceleration, especially upon turning of the vehicle. Therefore, this paper proposes a practical optimal speed control method to greatly improve the ride comfort in the vehicle turning situation. For consturcting this method, effective criteria that can appropriately evaluate deterioration of ride comfort is derived. The method can reduce the influence of both the longitudinal and the lateral speed changes for providing a confortable ride. From several simulation results, we can see the fact that the method can prevent aggravation of the ride comfort by suppressing the influence of longitudinal speed change in the turning situation. Hence, the effectiveness of the method is recognized. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electric%20vehicle" title="electric vehicle">electric vehicle</a>, <a href="https://publications.waset.org/abstracts/search?q=speed%20control" title=" speed control"> speed control</a>, <a href="https://publications.waset.org/abstracts/search?q=ride%20comfort" title=" ride comfort"> ride comfort</a>, <a href="https://publications.waset.org/abstracts/search?q=optimal%20control%20theory" title=" optimal control theory"> optimal control theory</a>, <a href="https://publications.waset.org/abstracts/search?q=driving%20support%20system" title=" driving support system"> driving support system</a> </p> <a href="https://publications.waset.org/abstracts/97046/improvement-of-ride-comfort-of-turning-electric-vehicle-using-optimal-speed-control" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/97046.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">215</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Slip Suppression Sliding Mode Control with Various Chattering Functions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shun%20Horikoshi">Shun Horikoshi</a>, <a href="https://publications.waset.org/abstracts/search?q=Tohru%20Kawabe"> Tohru Kawabe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study presents performance analysis results of SMC (Sliding mode control) with changing the chattering functions applied to slip suppression problem of electric vehicles (EVs). In SMC, chattering phenomenon always occurs through high frequency switching of the control inputs. It is undesirable phenomenon and degrade the control performance, since it causes the oscillations of the control inputs. Several studies have been conducted on this problem by introducing some general saturation function. However, study about whether saturation function was really best and the performance analysis when using the other functions, weren鈥檛 being done so much. Therefore, in this paper, several candidate functions for SMC are selected and control performance of candidate functions is analyzed. In the analysis, evaluation function based on the trade-off between slip suppression performance and chattering reduction performance is proposed. The analyses are conducted in several numerical simulations of slip suppression problem of EVs. Then, we can see that there is no difference of employed candidate functions in chattering reduction performance. On the other hand, in slip suppression performance, the saturation function is excellent overall. So, we conclude the saturation function is most suitable for slip suppression sliding mode control. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sliding%20mode%20control" title="sliding mode control">sliding mode control</a>, <a href="https://publications.waset.org/abstracts/search?q=chattering%20function" title=" chattering function"> chattering function</a>, <a href="https://publications.waset.org/abstracts/search?q=electric%20vehicle" title=" electric vehicle"> electric vehicle</a>, <a href="https://publications.waset.org/abstracts/search?q=slip%20suppression" title=" slip suppression"> slip suppression</a>, <a href="https://publications.waset.org/abstracts/search?q=performance%20analysis" title=" performance analysis"> performance analysis</a> </p> <a href="https://publications.waset.org/abstracts/75656/slip-suppression-sliding-mode-control-with-various-chattering-functions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75656.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">326</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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