Pathological and Molecular Diagnosis of Caseous Lymphadenitis in Chinkara Deer (Gazella Bennettii), in Pakistan

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Diagnosis of Caseous Lymphadenitis in Chinkara Deer (Gazella Bennettii), in Pakistan</title> <meta name="description" content="Pathological and Molecular Diagnosis of Caseous Lymphadenitis in Chinkara Deer (Gazella Bennettii), in Pakistan"> <meta name="keywords" content="Chinkara deer, Corynebacterium pseudotuberculosis, Caseous lymphadenitis, PCR"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" 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</div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Pathological and Molecular Diagnosis of Caseous Lymphadenitis in Chinkara Deer (Gazella Bennettii), in Pakistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mudassar%20Iqbal">Mudassar Iqbal</a>, <a href="https://publications.waset.org/abstracts/search?q=Riaz%20Hussain"> Riaz Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Khalid%20Mehmood"> Khalid Mehmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Farah%20Ali"> Farah Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Fazal%20Mahmood"> Fazal Mahmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdul%20Ghaffar"> Abdul Ghaffar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Corynebacterium pseudotuberculosis is an important cause of caseous lymphadenitis (CL), a complex, chronic devastating and destructive disease of small ruminants. In present study, postmortem examination of Chinkara deer (n=25) was conducted in year 2014. Pus samples suggestive of CL were collected from the superficial lymph nodes, liver, spleen and lungs during necropsy and subjected to standard microbiological procedures for isolation and molecular analysis of bacterial pathogens. Pus samples collected from carcasses (25) presenting clinical lesions of C. pseudotuberculosis infection was identified in 19 (76%) carcasses on the basis of culture characteristics. The frequency of C. pseudotuberculosis bacterium was higher in older animals as compared to young animals. Grossly, multiple tubercles of variable size having caseous material were observed in liver, lungs, spleen and lymph nodes. Histopathologically, tissue sections from all the visceral organs were extensively plugged with abscess. In present study specific prolineiminopeptidase (PIP) gene of the C. pseudotuberculosis was amplified by the Polymerase chain reaction technique (PCR) in 17(25) cases. The efficient and reliable molecular analysis along with necropsy findings in present study can be used as valuable approach for diagnosis of caseous lymphadenitis in small ruminants. <iframe src="https://publications.waset.org/abstracts/24616.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chinkara%20deer" title="Chinkara deer">Chinkara deer</a>, <a href="https://publications.waset.org/abstracts/search?q=Corynebacterium%20pseudotuberculosis" title=" Corynebacterium pseudotuberculosis"> Corynebacterium pseudotuberculosis</a>, <a href="https://publications.waset.org/abstracts/search?q=Caseous%20lymphadenitis" title=" Caseous lymphadenitis"> Caseous lymphadenitis</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a> </p> <a href="https://publications.waset.org/abstracts/24616/pathological-and-molecular-diagnosis-of-caseous-lymphadenitis-in-chinkara-deer-gazella-bennettii-in-pakistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24616.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">482</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational 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Pathological and Molecular Diagnosis of Caseous Lymphadenitis in Chinkara Deer (Gazella Bennettii), in Pakistan